Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0004500

Physical Location & Seq

Genomic Coordinates Reverse (-)

27998621 .. 28014019

Gene Structure 15399 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1217300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1185 bp

ATGGGATCGTCGAGATTGAGACTGTTCGCGAAGGCCATTTGTGATTCCGCTCGTCATCGTCATCGTCATCCTCATCCTCATCGCCATCACAGGATTCTACATCCAACTCACGCTCACACCCTCAGCGAGATGCATAGCTTGTCGAAAATGGAAGAAGAAAATGGCGAGGTGAAGAACAAGGAGAAAATTACCCGTCTTCTTTTCTGCGGATCACATTTTCCTGCTGCTGAAACTTACACAAGGGAATATTTGAAAGACTACCCCTTTATCCAGGTAGATGATGTTCCCCATAAAGATGTGCCTGGTGTTATCCAGAATTACAGTATGTGTATCGTGAAGATGATGAAGTTAGATTCAGATATTCTCTCCCGTGCAGAAAAGATGAAGCTTGTACTGCAGTATGGTGTCGGCCTGGAAGGCGTTGACATTGATTCTGCAACTAAGTCTGGAATCAAAGTTGCTAGGATTCCGAGCCATGTAACTGGAAATGCAGCCTCATGTGCCGAAATGGCCATTTATCTGATGTTGGGCCTCCTACGAAAGCAAAATGAGATGCAAATTTCTATTAAGCTGGGGAAGGTTGGGGATCCAGTTGGTGAAACACTACTTGGAAAAACAGTTTTCATTTTAGGATATGGGAACATTGGAATTGAATTGGCAAAGCGCTTGAGACCGTTTGGTGTGAAAATTATTGCCTCCAAACGGAGTTGGGCCACACAGTCACAAGATTCTTGCCAATCAAATGTTCAAAATTGTTCCACTGAGAATTTGGTTGATGAGAAGTGTGTCCATGAAGATATCCACAAGTTTGCAAGTATTGCAGACATCGTTGTTTGCTGCGTGCATTTAAACAGTGAAACAGTTGGCATTGTAAACAAGTCGTTCATATCTTCGATGAGAAAGGGTGCCCTTCTGGTAAATATTGCCCGAGGGGGTCTCCTGGACTATGAAGCTGTTTCGTATAGCCTAGAGTCTGGCCACTTAGGTGGTTTAGGGATTGATGTTGCTTGGACGGAGCCATTTGATCCTGACGATCCAATTTTGAAGTTCAACAATGTTATCATCACACCTCATGTGGCAGGAGTTACAGAGTATTCTTACAGATCCATGGCCAAGGTAGTTGGCGATGTCGCCATTCAACTTCATGAAGGGAAGCCTTTGACAGGAATTGAGTTTGTCAACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0003008 GO:0003674 GO:0003824 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006081 GO:0006082 GO:0007588 GO:0008150 GO:0008152 GO:0008465 GO:0009987 GO:0016043 GO:0016491 GO:0016614 GO:0016616 GO:0016618 GO:0019752 GO:0022607 GO:0030267 GO:0031406 GO:0032501 GO:0032787 GO:0036094 GO:0042802 GO:0042803 GO:0043167 GO:0043168 GO:0043177 GO:0043436 GO:0043648 GO:0043933 GO:0044085 GO:0044237 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046487 GO:0046983 GO:0048037 GO:0050661 GO:0050662 GO:0051259 GO:0051287 GO:0055114 GO:0065003 GO:0070402 GO:0071704 GO:0071840 GO:0097159 GO:1901265 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

2-Hacid_dh 2-Hacid_dh_C

Protein Analysis

395

Amino Acids

43.6

Weight (kDa)

7.68

Isoelectric Point (pI)

37.79

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
2-Hacid_dh	PF00389	77 - 387	1.8e-13	D-isomer specific 2-hydroxyacid dehydrogenase, catalytic domain
2-Hacid_dh_C	PF02826	173 - 360	4.3e-40	D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004500)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G72190
fragaria_vesca	FvH4_5g31330 FvH4_5g31330 FvH4_5g31330 FvH4_5g31330 FvH4_5g31330 FvH4_5g31330 FvH4_5g31330
malus_domestica	MD08G1217300.v1.1
prunus_persica	Prupe.1G556800_v2.0.a1 Prupe.1G556800_v2.0.a1 Prupe.1G556800_v2.0.a1 Prupe.1G556800_v2.0.a1 Prupe.1G556800_v2.0.a1 Prupe.1G556800_v2.0.a1
pyrus_communis	pycom08g18800
rosa_chinensis	RchiOBHm_Chr7g0226521 RchiOBHm_Chr7g0226551
rosa_laevigata	RLG00000001716 RLG00000001719
rosa_multiflora	Rmu_sc0001423.1_g000008
rosa_roxburghii	Rroxscaffold_3G00232710
rosa_rugosa	Rorug07G0237900 Rorug07G0237900 Rorug07G0238000 Rorug07G0238200
rosa_samantha	Rh7BG372200 Rh7BG372500 Rh7DG384300 Rh7DG384500
rosa_wichuraiana	Rw7G032370 Rw7G032390

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	905
AccBSI	CCGCTC	1 cut(s)	50
AccII	CGCG	1 cut(s)	29
AciI	CCGC	2 cut(s)	48, 207
AclWI	GGATC	7 cut(s)	13, 217, 581, 594, 1019, 1028, 1098
AcoI	YGGCCR	3 cut(s)	510, 976, 1110
AcsI	RAATTY	2 cut(s)	558, 766
AfaI	GTAC	1 cut(s)	393
AfeI	AGCGCT	1 cut(s)	665
AfiI	CCNNNNNNNGG	2 cut(s)	702, 1163
AgsI	TTSAA	6 cut(s)	253, 653, 749, 1045, 1051, 1139
AjnI	CCWGG	4 cut(s)	270, 301, 411, 939
AjuI	GAANNNNNNNTTGG	4 cut(s)	591, 623, 729, 761
AleI	CACNNNNGTG	1 cut(s)	984
AluBI	AGCT	4 cut(s)	138, 388, 571, 953
AluI	AGCT	4 cut(s)	138, 388, 571, 953
Alw26I	GTCTC	3 cut(s)	13, 664, 941
AlwI	GGATC	7 cut(s)	13, 217, 581, 594, 1019, 1028, 1098
Ama87I	CYCGRG	1 cut(s)	927
Aor51HI	AGCGCT	1 cut(s)	665
AoxI	GGCC	7 cut(s)	33, 409, 510, 529, 711, 976, 1110
ApeKI	GCWGC	3 cut(s)	224, 491, 837
ApoI	RAATTY	2 cut(s)	558, 766
AspLEI	GCGC	1 cut(s)	666
AspS9I	GGNCC	2 cut(s)	529, 711
AsuHPI	GGTGA	2 cut(s)	181, 608
AvaI	CYCGRG	1 cut(s)	927
BaeGI	GKGCMC	1 cut(s)	910
BalI	TGGCCA	3 cut(s)	512, 978, 1112
BamHI	GGATCC	1 cut(s)	586
BanI	GGYRCC	1 cut(s)	905
BbsI	GAAGAC	1 cut(s)	188
BbvCI	CCTCAGC	1 cut(s)	122
BbvI	GCAGC	3 cut(s)	211, 503, 824
BccI	CCATC	1 cut(s)	93
BciT130I	CCWGG	4 cut(s)	272, 303, 413, 941
BcoDI	GTCTC	3 cut(s)	13, 664, 941
BfaI	CTAG	2 cut(s)	462, 968
BfmI	CTRYAG	1 cut(s)	395
BfoI	RGCGCY	1 cut(s)	667
BglI	GCCNNNNNGGC	2 cut(s)	417, 509
BisI	GCNGC	3 cut(s)	225, 492, 838
BlsI	GCNGC	3 cut(s)	226, 493, 839
Bme1390I	CCNGG	4 cut(s)	272, 303, 413, 941
BmeT110I	CYCGRG	1 cut(s)	927
BmgT120I	GGNCC	2 cut(s)	529, 711
BmiI	GGNNCC	3 cut(s)	588, 907, 1017
BmrFI	CCNGG	4 cut(s)	272, 303, 413, 941
BmsI	GCATC	2 cut(s)	120, 543
BpiI	GAAGAC	1 cut(s)	188
BplI	GAGNNNNNCTC	2 cut(s)	921, 953
Bpu10I	CCTNAGC	1 cut(s)	122
BpuEI	CTTGAG	1 cut(s)	688
BsaI	GGTCTC	2 cut(s)	664, 941
BsaJI	CCNNGG	3 cut(s)	928, 1107, 1113
Bsc4I	CCNNNNNNNGG	2 cut(s)	702, 1163
Bse1I	ACTGG	2 cut(s)	487, 590
BseBI	CCWGG	4 cut(s)	272, 303, 413, 941
BseDI	CCNNGG	3 cut(s)	928, 1107, 1113
BseGI	GGATG	3 cut(s)	67, 73, 100
BseLI	CCNNNNNNNGG	2 cut(s)	702, 1163
BseMII	CTCAG	2 cut(s)	136, 753
BseNI	ACTGG	2 cut(s)	487, 590
BseSI	GKGCMC	1 cut(s)	910
BseXI	GCAGC	3 cut(s)	211, 503, 824
BseYI	CCCAGC	1 cut(s)	571
BsgI	GTGCAG	1 cut(s)	393
Bsh1236I	CGCG	1 cut(s)	29
BshFI	GGCC	7 cut(s)	35, 411, 512, 531, 713, 978, 1112
BshNI	GGYRCC	1 cut(s)	905
BsiHKCI	CYCGRG	1 cut(s)	927
BslI	CCNNNNNNNGG	2 cut(s)	702, 1163
BsmAI	GTCTC	3 cut(s)	13, 664, 941
BsnI	GGCC	7 cut(s)	35, 411, 512, 531, 713, 978, 1112
Bso31I	GGTCTC	2 cut(s)	664, 941
BsoBI	CYCGRG	1 cut(s)	927
Bsp1286I	GDGCHC	1 cut(s)	910
Bsp143I	GATC	6 cut(s)	5, 209, 586, 1024, 1033, 1103
Bsp19I	CCATGG	1 cut(s)	1107
Bsp68I	TCGCGA	1 cut(s)	29
BspACI	CCGC	2 cut(s)	48, 207
BspANI	GGCC	7 cut(s)	35, 411, 512, 531, 713, 978, 1112
BspCNI	CTCAG	2 cut(s)	135, 754
BspFNI	CGCG	1 cut(s)	29
BspHI	TCATGA	1 cut(s)	1144
BspLI	GGNNCC	3 cut(s)	588, 907, 1017
BspMAI	CTGCAG	1 cut(s)	399
BspPI	GGATC	7 cut(s)	13, 217, 581, 594, 1019, 1028, 1098
BspT107I	GGYRCC	1 cut(s)	905
BspTNI	GGTCTC	2 cut(s)	664, 941
BsrBI	CCGCTC	1 cut(s)	50
BsrI	ACTGG	2 cut(s)	487, 590
BssECI	CCNNGG	3 cut(s)	928, 1107, 1113
BssMI	GATC	6 cut(s)	5, 209, 586, 1024, 1033, 1103
BssT1I	CCWWGG	2 cut(s)	1107, 1113
Bst2UI	CCWGG	4 cut(s)	272, 303, 413, 941
Bst4CI	ACNGT	7 cut(s)	24, 323, 619, 675, 720, 854, 862
BstAPI	GCANNNNNTGC	1 cut(s)	818
BstC8I	GCNNGC	1 cut(s)	842
BstDEI	CTNAG	4 cut(s)	122, 441, 762, 982
BstDSI	CCRYGG	1 cut(s)	1107
BstENI	CCTNNNNNAGG	1 cut(s)	1161
BstF5I	GGATG	3 cut(s)	67, 73, 100
BstFNI	CGCG	1 cut(s)	29
BstH2I	RGCGCY	1 cut(s)	667
BstHHI	GCGC	1 cut(s)	666
BstKTI	GATC	6 cut(s)	8, 212, 589, 1027, 1036, 1106
BstMAI	GTCTC	3 cut(s)	13, 664, 941
BstMBI	GATC	6 cut(s)	5, 209, 586, 1024, 1033, 1103
BstMWI	GCNNNNNNNGC	5 cut(s)	394, 417, 500, 509, 818
BstNI	CCWGG	4 cut(s)	272, 303, 413, 941
BstSCI	CCNGG	4 cut(s)	270, 301, 411, 939
BstSFI	CTRYAG	1 cut(s)	395
BstSLI	GKGCMC	1 cut(s)	910
BstUI	CGCG	1 cut(s)	29
BstV1I	GCAGC	3 cut(s)	211, 503, 824
BstV2I	GAAGAC	1 cut(s)	188
BstX2I	RGATCY	2 cut(s)	586, 1103
BstXI	CCANNNNNNTGG	2 cut(s)	482, 986
BstYI	RGATCY	2 cut(s)	586, 1103
BsuRI	GGCC	7 cut(s)	35, 411, 512, 531, 713, 978, 1112
BtgI	CCRYGG	1 cut(s)	1107
BtgZI	GCGATG	2 cut(s)	65, 1140
BtsCI	GGATG	3 cut(s)	67, 73, 100
BtsIMutI	CAGTG	2 cut(s)	759, 859
BtuMI	TCGCGA	1 cut(s)	29
Cac8I	GCNNGC	1 cut(s)	842
CciI	TCATGA	1 cut(s)	1144
CfoI	GCGC	1 cut(s)	666
Cfr13I	GGNCC	2 cut(s)	529, 711
Csp6I	GTAC	1 cut(s)	392
CviAII	CATG	6 cut(s)	476, 498, 791, 1073, 1108, 1145
CviQI	GTAC	1 cut(s)	392
DdeI	CTNAG	4 cut(s)	122, 441, 762, 982
DpnI	GATC	6 cut(s)	7, 211, 588, 1026, 1035, 1105
DpnII	GATC	6 cut(s)	5, 209, 586, 1024, 1033, 1103
DraI	TTTAAA	1 cut(s)	849
EaeI	YGGCCR	3 cut(s)	510, 976, 1110
Eco130I	CCWWGG	2 cut(s)	1107, 1113
Eco31I	GGTCTC	2 cut(s)	664, 941
Eco32I	GATATC	1 cut(s)	799
Eco47III	AGCGCT	1 cut(s)	665
Eco88I	CYCGRG	1 cut(s)	927
EcoNI	CCTNNNNNAGG	1 cut(s)	1161
EcoRII	CCWGG	4 cut(s)	270, 301, 411, 939
EcoRV	GATATC	1 cut(s)	799
EcoT14I	CCWWGG	2 cut(s)	1107, 1113
EcoT22I	ATGCAT	1 cut(s)	135
ErhI	CCWWGG	2 cut(s)	1107, 1113
FaeI	CATG	6 cut(s)	479, 501, 794, 1076, 1111, 1148
FatI	CATG	6 cut(s)	475, 497, 790, 1072, 1107, 1144
Fnu4HI	GCNGC	3 cut(s)	225, 492, 838
FokI	GGATG	3 cut(s)	54, 60, 87
Fsp4HI	GCNGC	3 cut(s)	225, 492, 838
FspBI	CTAG	2 cut(s)	462, 968
GlaI	GCGC	1 cut(s)	665
GluI	GCNGC	3 cut(s)	225, 492, 838
GsaI	CCCAGC	1 cut(s)	575
HaeII	RGCGCY	1 cut(s)	667
HaeIII	GGCC	7 cut(s)	35, 411, 512, 531, 713, 978, 1112
HhaI	GCGC	1 cut(s)	666
Hin1II	CATG	6 cut(s)	479, 501, 794, 1076, 1111, 1148
Hin6I	GCGC	1 cut(s)	664
HinP1I	GCGC	1 cut(s)	664
HincII	GTYRAC	2 cut(s)	424, 1180
HindII	GTYRAC	2 cut(s)	424, 1180
HindIII	AAGCTT	1 cut(s)	386
HinfI	GANTC	8 cut(s)	44, 94, 353, 431, 450, 466, 728, 971
HphI	GGTGA	2 cut(s)	181, 608
Hpy166II	GTNNAC	3 cut(s)	424, 874, 1180
Hpy188I	TCNGA	3 cut(s)	358, 471, 522
Hpy188III	TCNNGA	7 cut(s)	12, 28, 313, 334, 447, 1028, 1145
Hpy8I	GTNNAC	3 cut(s)	424, 874, 1180
Hpy99I	CGWCG	1 cut(s)	13
HpyAV	CCTTC	5 cut(s)	25, 410, 571, 920, 1142
HpyCH4III	ACNGT	7 cut(s)	24, 323, 619, 675, 720, 854, 862
HpyCH4V	TGCA	9 cut(s)	133, 374, 397, 437, 491, 556, 812, 821, 844
HpyF10VI	GCNNNNNNNGC	5 cut(s)	394, 417, 500, 509, 818
HpyF3I	CTNAG	4 cut(s)	122, 441, 762, 982
Hsp92II	CATG	6 cut(s)	479, 501, 794, 1076, 1111, 1148
HspAI	GCGC	1 cut(s)	664
Kzo9I	GATC	6 cut(s)	5, 209, 586, 1024, 1033, 1103
LmnI	GCTCC	1 cut(s)	1015
Lsp1109I	GCAGC	3 cut(s)	211, 503, 824
LweI	GCATC	2 cut(s)	120, 543
MaeI	CTAG	2 cut(s)	462, 968
MaeIII	GTNAC	3 cut(s)	478, 720, 1084
MalI	GATC	6 cut(s)	7, 211, 588, 1026, 1035, 1105
MbiI	CCGCTC	1 cut(s)	50
MboI	GATC	6 cut(s)	5, 209, 586, 1024, 1033, 1103
MboII	GAAGA	7 cut(s)	164, 167, 184, 188, 349, 806, 882
MflI	RGATCY	2 cut(s)	586, 1103
MhlI	GDGCHC	1 cut(s)	910
MlsI	TGGCCA	3 cut(s)	512, 978, 1112
MluNI	TGGCCA	3 cut(s)	512, 978, 1112
MlyI	GAGTC	1 cut(s)	980
MmeI	TCCRAC	1 cut(s)	128
MnlI	CCTC	9 cut(s)	81, 87, 131, 160, 505, 542, 706, 923, 1080
Mox20I	TGGCCA	3 cut(s)	512, 978, 1112
Mph1103I	ATGCAT	1 cut(s)	135
MscI	TGGCCA	3 cut(s)	512, 978, 1112
MseI	TTAA	2 cut(s)	567, 848
MslI	CAYNNNNRTG	2 cut(s)	294, 984
Msp20I	TGGCCA	3 cut(s)	512, 978, 1112
MspR9I	CCNGG	4 cut(s)	272, 303, 413, 941
MvaI	CCWGG	4 cut(s)	272, 303, 413, 941
MvnI	CGCG	1 cut(s)	29
MwoI	GCNNNNNNNGC	5 cut(s)	394, 417, 500, 509, 818
NcoI	CCATGG	1 cut(s)	1107
NdeII	GATC	6 cut(s)	5, 209, 586, 1024, 1033, 1103
NlaIII	CATG	6 cut(s)	479, 501, 794, 1076, 1111, 1148
NlaIV	GGNNCC	3 cut(s)	588, 907, 1017
NmuCI	GTSAC	1 cut(s)	720
NruI	TCGCGA	1 cut(s)	29
NsiI	ATGCAT	1 cut(s)	135
OliI	CACNNNNGTG	1 cut(s)	984
PagI	TCATGA	1 cut(s)	1144
PfeI	GAWTC	7 cut(s)	44, 94, 353, 431, 450, 466, 728
PfoI	TCCNGGA	1 cut(s)	939
PkrI	GCNGC	3 cut(s)	226, 493, 839
PleI	GAGTC	1 cut(s)	979
PpsI	GAGTC	1 cut(s)	979
Psp6I	CCWGG	4 cut(s)	270, 301, 411, 939
PspFI	CCCAGC	1 cut(s)	571
PspGI	CCWGG	4 cut(s)	270, 301, 411, 939
PspN4I	GGNNCC	3 cut(s)	588, 907, 1017
PspPI	GGNCC	2 cut(s)	529, 711
PsrI	GAACNNNNNNTAC	2 cut(s)	267, 299
PstI	CTGCAG	1 cut(s)	399
PsuI	RGATCY	2 cut(s)	586, 1103
RruI	TCGCGA	1 cut(s)	29
RsaI	GTAC	1 cut(s)	393
RsaNI	GTAC	1 cut(s)	392
RseI	CAYNNNNRTG	2 cut(s)	294, 984
SaqAI	TTAA	2 cut(s)	567, 848
SatI	GCNGC	3 cut(s)	225, 492, 838
Sau3AI	GATC	6 cut(s)	5, 209, 586, 1024, 1033, 1103
Sau96I	GGNCC	2 cut(s)	529, 711
SchI	GAGTC	1 cut(s)	980
ScrFI	CCNGG	4 cut(s)	272, 303, 413, 941
SduI	GDGCHC	1 cut(s)	910
SfaNI	GCATC	2 cut(s)	120, 543
SfcI	CTRYAG	1 cut(s)	395
SmiMI	CAYNNNNRTG	2 cut(s)	294, 984
SmlI	CTYRAG	1 cut(s)	667
SmoI	CTYRAG	1 cut(s)	667
SsiI	CCGC	2 cut(s)	48, 207
SspI	AATATT	2 cut(s)	248, 922
SspMI	CTAG	2 cut(s)	462, 968
StyD4I	CCNGG	4 cut(s)	270, 301, 411, 939
StyI	CCWWGG	2 cut(s)	1107, 1113
TaaI	ACNGT	7 cut(s)	24, 323, 619, 675, 720, 854, 862
TaqI	TCGA	3 cut(s)	11, 143, 893
TatI	WGTACW	1 cut(s)	391
TfiI	GAWTC	7 cut(s)	44, 94, 353, 431, 450, 466, 728
Tru1I	TTAA	2 cut(s)	567, 848
Tru9I	TTAA	2 cut(s)	567, 848
TscAI	CASTG	2 cut(s)	766, 859
TseFI	GTSAC	1 cut(s)	720
TseI	GCWGC	3 cut(s)	224, 491, 837
Tsp45I	GTSAC	1 cut(s)	720
TspDTI	ATGAA	8 cut(s)	359, 398, 613, 807, 874, 963, 1133, 1161
TspGWI	ACGGA	2 cut(s)	718, 1028
TspRI	CASTG	2 cut(s)	766, 859
XagI	CCTNNNNNAGG	1 cut(s)	1161
XapI	RAATTY	2 cut(s)	558, 766
XcmI	CCANNNNNNNNNTGG	1 cut(s)	766
XspI	CTAG	2 cut(s)	462, 968
Zsp2I	ATGCAT	1 cut(s)	135

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.