Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0005762

Physical Location & Seq

Genomic Coordinates Reverse (-)

27922725 .. 27927962

Gene Structure 5238 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT1G74260.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4224 bp

ATGAATACCTCCCAAGCAACTCGTGCGGCTCTGTTTCTAAACGGTTCCAATAGACAAGCGATGCTTTTGCAGCGGAGCTCAATGAGTCAATTGTGGGGTTCTGTTAGAATGAGAACTTCACGGCTGTCTCTGAATCGAACAAAAGCTGTTAGCTTGAGATGTTCTGCTCAACCTAATAAACCTAAAGCAGCTGTTTCTACTGGTTCTTTTGTTACTGCTGATGAGCTACCAAGCTTGGTTGAGAAACCTGCTGCTGAGGTTATCCATTTTTATCGCGTGCCTTTGATTCAAGAAAGCGCGAACGCGGAACTCCTCAAGGCTGTTCAAACCAAAATCAGCAACCAGATTGTCAGTTTGACTACAGAACAGTCTTTCAATATTGGGCTTGAATCCAAGTTAAAAGATGAAAAACTTTCTGTTCTAAAGTGGATTCTTCAAGAAACATATGAGCCAGAGAATTTAGGTACGGATAGTTTTCTTGAGAGGAAGAAGCAAGAAGGACTCCATGCTGTTATTGTTGAAGTTGGCCCCAGATTGTCTTTCACAACAGCATGGTCCACCAATGCGGTTTCGATATGTAGAGCATGTGGGTTAGACGAGGTGACTCGCTTGGAAAGGTCAAGGAGGTACCTTTTGTTCAGCAAGGAGCCACTTTTGGAAAATCAGATAAAAGAATTTGCTGCAATGGTTCATGATAGAATGACCGAGTGCGTGTACACTCAAAAGCTGGTTTCATTCGAGACGAATGTGGTTCCAGAAGAAGTGAAGTATGTGCCTGTGATGGAGAAGGGTCGGAAGGCGCTGGAAGAAATCAACCAGGAGATGGGTTTAGCCTTTGATGAGCAAGATTTGCAGTATTATACTAGACTTTTCAGAGAGGACATTAAGCGTGATCCTACCAATGTGGAGCTATTTGATATCGCTCAATCCAACAGCGAGCACAGTCGACACTGGTTCTTTGCTGGGAACATGGTTATTGATGGAAAGCCAATGGATAAGTCTCTTATGCAGATTGTAAAGAGCACTTGGGAGGCAAATCGAAATAATTCTGTCATTGGGTTTAAGGATAACTCTAGTGCTATTAGAGGCTTTCTGGTGAACCAGCTACGGCCTCTACTTCCTGGTTCTGTGTGCTTACTTGATGTCAGTGCACGTGATCTCGACATATTGTTCACTGCTGAAACCCATAATTTCCCTTGTGCTGTGGCTCCTTATCCTGGTGCTGAGACAGGTGCTGGAGGTCGAATCAGAGATACACATGCAACAGGAAGAGGTTCTTTTGTGGTTGCATCCACTTCTGGTTACTGTGTTGGTAACCTTAACATGGAGGGATCTTATGCTCCATGGGAAGACTCGTCTTTCCAATACCCATCGAACCTAGCATCACCTCTACAGATACTCATCGACGCTAGTAATGGTGCATCTGACTATGGTAACAAATTTGGAGAGCCCATGATTCAGGGATATACTAGGACTTTTGGAATGAGACTGCCAAGTGGGGATAGACGAGAATGGTTGAAGCCGATTATGTTCAGTGCAGGGATTGGACAGATTGATCATACTCATATAACTAAAGGGGAGCCAGAGGTTGGGATGCTTGTTGTTAAGATTGGTGGACCTGCTTACCGCATTGGCATGGGAGGAGGAGCAGCTTCGAGTATGGTTAGTGGCCAGAATGATGCAGAGCTTGATTTCAATGCTGTGCAACGTGGAGATGCTGAAATGTCTCAGAAGTTGTACCGTGTTGTTCGTGCTTGTATTGAGATGGGTGAGAAGAATCCTATTATCAGCATTCATGATCAAGGTGCTGGTGGAAACTGCAATGTTGTCAAGGAAATTATTTATCCACAGGGTGCAGAGATTGACATAAGAGCGGTTGTTGTGGGTGATCATACTATGTCGGTGTTGGAGATATGGGGAGCAGAATATCAAGAGCAAGATGCGATTTTGGTGAAAGCTGAGAGCCGGGAGATTTTGCAATCAATCTGTAAGAGGGAAAGGCTTTCGATGGCTGTGATTGGAACAATTAATGGGGGTGGTCGCTGTACTTTAATTGACAGCACAGCTGCAGCGAAGTGCAGTAAAGAAGGGCTACCTCCACCTCCACCTGCTGTGGATCTTGAACTCGAGAAGGTTCTTGGTGATATGCCTAAGAAGACGTTTAAGTTCAACCGCATTGCTTATGCACGGGAGCCACTTGATATTGCTCCTGGAATTACATTGATGGATGCTTTGAAAAGAGTTCTCCGATTACCATCAGTTTCTTCAAAGCGGTTCTTGACAACCAAAGTGGATAGATGTGTGACAGGTCTTGTTGCTCAGCAACAAACAGTTGGGCCATTGCAGATCACGCTTGCTGATGTTGCAGTTATTGCACAGACATTCACAGATCTAACAGGTGGTGCATGTGCCATTGGTGAGCAACCGATCAAAGGCTTGCTTGATCCAAAAGCCATGGCTAGGCTAGCTGTTGGAGAGGCTTTGACAAATCTGGTTTGGGCAAAGGTCACTGCACTTTCTGATGTTAAAGCTAGTGGTAACTGGATGTATGCTGCCAAGCTTGAAGGAGAAGGATCAGCAATGTATGATGCTGCGATTGCTCTATCTGAAGCGATGATTGAACTTGGCATTGCAATTGATGGTGGAAAAGACAGTCTTTCAATGGCAGCTCATGCGGACGGTGAGGTTGTTAAAGCTCCAGGAAATCTTGTGATTAGTGCCTATGTTACCTGTCCAGACATAACAAAGACAGTGACTCCGGATCTAAAGCTCGGAGGTGATGATGGTATTCTCTTGCATGTTGATTTGGCAAAGGGAAAGAGGAGATTGGGTGGATCTGCACTGGCTCAGGTTTTTGGTCAGATAGGAAATGACTGTCCTGATCTTGATGATGTTCCATATCTGAAAAACGTTTTCGATGGCGTTCAAGCTCTCATTGCAGAAAACTTGGTATCTGCTGGACACGACATCAGTGATGGTGGACTGGTAGTAACAGCTTTGGAAATGGCTTTTGCTGGAAACAAAGGTATAAATCTCGACTTGGCTTCAAATGGAATTAGCCTTTTTGAGACTTTGTTTTCTGAAGAACTTGGTCTCGTGCTTGAGATTAGTAAGACAAACCTGGACGCTGTGATGGAAAAACTCCGTGCTTTCGATGTTACTGCTGAGATCATTGGAAATGTCACTGATTCGCCTCTGATAGAGGTAAAAGTGGATGGGATTACTCATTTGAGTGAGAAAACTTCATTCCTCAGAGACATGTGGGAAGACACCAGTTTCCAGTTGGAAAAGCTGCAACGATTGGCATCTTGTGTAGAGATGGAGAAAGAAGGTTTGAAGTTTAGGCATGAGCCTAATTGGAAACTCTCATTTATTCCATCCTCGACCAACAATAATTATATGTCTCAGGATGTTAAGCCAAAAGTAGCAGTGATCCGAGAAGAAGGCAGCAATGGAGACAGAGAAATGTCAGCTGCATTTTACGCTGCCGGTTTTGAACCTTGGGACGTGACAGTGTCTGATCTTCTAGCTGGAGACATCACCCTTGATCAGTTCCGCGGTATTGTGTTTGTGGGAGGGTTCAGTTATGCGGATGTTCTCGACTCAGCCAAAGGATGGGCTGCTTCAATAAGGTTCAACGAGCCTGTGTTGAGTCAATTTCAGGAGTTCTACAAAAGACCAGACACGTTCAGTCTTGGAATCTGCAATGGATGTCAGTTAATGGCTCTGTTAGGATGGGTTCCAGGTCCTCAAGTTGGCGGGTCACTTGACACCTCGCAGCCGAGGTTTGTTCACAACGAATCAGGAAGGTTTGAGTGCAGGTTCACAAGCGTGACCATAAAGGACTCGCCATCGATAATGCTGAAAGGAATGGAGGGAAGTACTTTAGGGGTTTGGGCGGCGCACGGAGAAGGACGGGCTTATTTCCCGGACGAAGGAGTCTTGGACCATATGCTTCACTCAGATTTGGCACCATTGAGATACTGTGATGATGATGGGAACGTGACTGAAGCGTACCCTTTTAACCTCAATGGTTCACCGTTGGGAATAGCGGCTATATGTTCACCTGATGGGAGACATTTGGCGATGATGCCTCATCCTGAACGTTGTTTCTTGATGTGGCAGTTTCCATGGTACCCAACAAGCTGGGACGTTGAGAAAGCTGGCCCGAGCCCGTGGTTGAAGATGTTCCAGAATGCAAGGGACTGGTTAGAGTCGTGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0003674 GO:0003824 GO:0004642 GO:0005488 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0007275 GO:0008144 GO:0008150 GO:0009507 GO:0009532 GO:0009536 GO:0009555 GO:0009570 GO:0016874 GO:0016879 GO:0016884 GO:0017076 GO:0030554 GO:0032501 GO:0032502 GO:0032553 GO:0032555 GO:0032559 GO:0035639 GO:0036094 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0048229 GO:0048856 GO:0055046 GO:0097159 GO:0097367 GO:1901265 GO:1901363

KEGG Pathways

Metabolic & Signaling

ko00230 ko01100 ko01110 ko01130 map00230 map01100 map01110 map01130

Pfam Domains

Protein Families

AIRS_C GATase_5

Protein Analysis

1407

Amino Acids

153.95

Weight (kDa)

5.21

Isoelectric Point (pI)

35.98

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FGAR-AT_N	PF18076	126 - 241	2e-20	Formylglycinamide ribonucleotide amidotransferase N-terminal
FGAR-AT_linker	PF18072	268 - 317	8.9e-11	Formylglycinamide ribonucleotide amidotransferase linker domain
AIRS_C	PF02769	529 - 685	5.2e-29	AIR synthase related protein, C-terminal domain
FGAR-AT_PurM_N-like	PF22689	756 - 909	9e-53	FGAR-AT PurM_N-like domain
AIRS_C	PF02769	941 - 1071	3.4e-22	AIR synthase related protein, C-terminal domain
GATase_5	PF13507	1139 - 1404	4.6e-108	CobB/CobQ-like glutamine amidotransferase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0005762)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G74260
fragaria_vesca	FvH4_5g19530 FvH4_5g19530 FvH4_5g19530
malus_domestica	MD03G1203100.v1.1 MD04G1022300.v1.1
prunus_persica	Prupe.1G542100_v2.0.a1 Prupe.1G542100_v2.0.a1 Prupe.1G542100_v2.0.a1 Prupe.1G542100_v2.0.a1 Prupe.1G542100_v2.0.a1 Prupe.8G024000_v2.0.a1 Prupe.8G024000_v2.0.a1 Prupe.8G024000_v2.0.a1
pyrus_communis	pycom04g01890 pycom04g01930
rosa_chinensis	RchiOBHm_Chr7g0204141
rosa_laevigata	RLG00000003485
rosa_multiflora	Rmu_sc0001311.1_g000031
rosa_roxburghii	Rroxscaffold_3G00253310
rosa_rugosa	Rorug07G0081100 Rorug07G0081200 Rorug07G0081200
rosa_samantha	Rh7AG211000 Rh7BG209600 Rh7CG223500 Rh7DG217800
rosa_wichuraiana	Rw7G018360

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	2116
AasI	GACNNNNNNGTC	1 cut(s)	3938
Acc36I	ACCTGC	4 cut(s)	256, 1627, 2116, 3810
Acc65I	GGTACC	2 cut(s)	627, 4134
AccB1I	GGYRCC	3 cut(s)	627, 3970, 4134
AccB7I	CCANNNNNTGG	3 cut(s)	823, 1589, 3615
AccBSI	CCGCTC	1 cut(s)	1874
AccI	GTMKAC	1 cut(s)	946
AccII	CGCG	4 cut(s)	276, 299, 305, 3558
AccIII	TCCGGA	1 cut(s)	2758
AclI	AACGTT	2 cut(s)	2910, 4105
AclWI	GGATC	8 cut(s)	887, 1339, 2124, 2438, 2581, 2769, 2842, 3427
AcoI	YGGCCR	1 cut(s)	1669
AcsI	RAATTY	3 cut(s)	457, 674, 1439
AcuI	CTGAAG	3 cut(s)	2628, 3102, 4029
AcvI	CACGTG	1 cut(s)	1154
AdeI	CACNNNGTG	1 cut(s)	1853
AfaI	GTAC	8 cut(s)	466, 629, 716, 1739, 2047, 3883, 4016, 4136
AfiI	CCNNNNNNNGG	9 cut(s)	823, 1217, 1324, 1589, 2432, 2460, 3615, 3755, 3935
AflIII	ACRYGT	2 cut(s)	3258, 3684
AjiI	CACGTC	1 cut(s)	3508
AjnI	CCWGG	7 cut(s)	816, 1120, 1216, 2209, 2698, 3120, 3742
AleI	CACNNNNGTG	1 cut(s)	3830
Alw21I	GWGCWC	4 cut(s)	80, 942, 1025, 1153
Alw44I	GTGCAC	1 cut(s)	1149
AlwI	GGATC	8 cut(s)	887, 1339, 2124, 2438, 2581, 2769, 2842, 3427
AlwNI	CAGNNNCTG	2 cut(s)	1235, 3518
Ama87I	CYCGRG	2 cut(s)	2126, 4168
Aor13HI	TCCGGA	1 cut(s)	2758
AoxI	GGCC	5 cut(s)	526, 1109, 1669, 2336, 4165
ApaLI	GTGCAC	1 cut(s)	1149
ApoI	RAATTY	3 cut(s)	457, 674, 1439
AseI	ATTAAT	1 cut(s)	2028
Asp700I	GAANNNNTTC	3 cut(s)	1045, 1273, 4187
Asp718I	GGTACC	2 cut(s)	627, 4134
AspLEI	GCGC	3 cut(s)	299, 802, 3904
AspS9I	GGNCC	7 cut(s)	527, 555, 1616, 2336, 3746, 3946, 4166
AsuC2I	CCSGG	2 cut(s)	1967, 3929
AsuNHI	GCTAGC	1 cut(s)	2464
AvaI	CYCGRG	2 cut(s)	2126, 4168
AvaII	GGWCC	4 cut(s)	555, 1616, 3746, 3946
BaeGI	GKGCMC	1 cut(s)	1153
BalI	TGGCCA	1 cut(s)	1671
BanI	GGYRCC	3 cut(s)	627, 3970, 4134
BanII	GRGCYC	3 cut(s)	80, 1452, 4175
BauI	CACGAG	2 cut(s)	21, 3095
BbrPI	CACGTG	1 cut(s)	1154
BbsI	GAAGAC	3 cut(s)	1356, 2162, 3273
Bbv12I	GWGCWC	4 cut(s)	80, 942, 1025, 1153
BbvCI	CCTCAGC	1 cut(s)	255
BceAI	ACGGC	2 cut(s)	137, 1124
BcgI	CGANNNNNNTGC	4 cut(s)	49, 83, 3143, 3177
BciT130I	CCWGG	7 cut(s)	818, 1122, 1218, 2211, 2700, 3122, 3744
BclI	TGATCA	4 cut(s)	1555, 1798, 1888, 3547
BcnI	CCSGG	2 cut(s)	1967, 3929
BfaI	CTAG	9 cut(s)	864, 1074, 1379, 1410, 1470, 2460, 2465, 2532, 3527
BfmI	CTRYAG	3 cut(s)	360, 1391, 2067
BfoI	RGCGCY	1 cut(s)	803
BfuAI	ACCTGC	4 cut(s)	256, 1627, 2116, 3810
BglII	AGATCT	1 cut(s)	2389
BlpI	GCTNAGC	1 cut(s)	2319
BmcAI	AGTACT	1 cut(s)	3883
Bme1390I	CCNGG	9 cut(s)	818, 1122, 1218, 1967, 2211, 2700, 3122, 3744, 3929
Bme18I	GGWCC	4 cut(s)	555, 1616, 3746, 3946
BmeT110I	CYCGRG	2 cut(s)	2126, 4168
BmgBI	CACGTC	1 cut(s)	3508
BmgT120I	GGNCC	7 cut(s)	527, 555, 1616, 2336, 3746, 3946, 4166
BmrFI	CCNGG	9 cut(s)	818, 1122, 1218, 1967, 2211, 2700, 3122, 3744, 3929
BmtI	GCTAGC	1 cut(s)	2468
BpiI	GAAGAC	3 cut(s)	1356, 2162, 3273
BpmI	CTGGAG	3 cut(s)	1257, 2682, 3552
Bpu10I	CCTNAGC	2 cut(s)	255, 2847
Bpu1102I	GCTNAGC	1 cut(s)	2319
BpuEI	CTTGAG	5 cut(s)	175, 299, 500, 3122, 3735
BpuMI	CCSGG	2 cut(s)	1967, 3929
Bsa29I	ATCGAT	1 cut(s)	3854
BsaAI	YACGTR	1 cut(s)	1154
BsaBI	GATNNNNATC	3 cut(s)	1400, 2254, 3304
BsaI	GGTCTC	1 cut(s)	3098
BsaJI	CCNNGG	7 cut(s)	1343, 2454, 3500, 3556, 3782, 4130, 4175
BsaWI	WCCGGW	1 cut(s)	2758
BsaXI	ACNNNNNCTCC	4 cut(s)	2740, 2770, 2815, 2845
Bsc4I	CCNNNNNNNGG	9 cut(s)	823, 1217, 1324, 1589, 2432, 2460, 3615, 3755, 3935
Bse118I	RCCGGY	1 cut(s)	3488
Bse1I	ACTGG	8 cut(s)	205, 956, 2546, 2847, 2988, 3273, 3280, 4211
Bse3DI	GCAATG	9 cut(s)	690, 1828, 2175, 2339, 2586, 2628, 2934, 3457, 3712
Bse8I	GATNNNNATC	3 cut(s)	1400, 2254, 3304
BseAI	TCCGGA	1 cut(s)	2758
BseBI	CCWGG	7 cut(s)	818, 1122, 1218, 2211, 2700, 3122, 3744
BseCI	ATCGAT	1 cut(s)	3854
BseDI	CCNNGG	7 cut(s)	1343, 2454, 3500, 3556, 3782, 4130, 4175
BseJI	GATNNNNATC	3 cut(s)	1400, 2254, 3304
BseLI	CCNNNNNNNGG	9 cut(s)	823, 1217, 1324, 1589, 2432, 2460, 3615, 3755, 3935
BseMI	GCAATG	9 cut(s)	690, 1828, 2175, 2339, 2586, 2628, 2934, 3457, 3712
BseNI	ACTGG	8 cut(s)	205, 956, 2546, 2847, 2988, 3273, 3280, 4211
BseRI	GAGGAG	4 cut(s)	302, 1656, 1659, 2836
BseSI	GKGCMC	1 cut(s)	1153
BseYI	CCCAGC	2 cut(s)	962, 4146
BsgI	GTGCAG	6 cut(s)	1557, 1875, 2098, 2496, 2823, 3838
Bsh1236I	CGCG	4 cut(s)	276, 299, 305, 3558
BshFI	GGCC	5 cut(s)	528, 1111, 1671, 2338, 4167
BshNI	GGYRCC	3 cut(s)	627, 3970, 4134
BshVI	ATCGAT	1 cut(s)	3854
BsiHKAI	GWGCWC	4 cut(s)	80, 942, 1025, 1153
BsiHKCI	CYCGRG	2 cut(s)	2126, 4168
BsiSI	CCGG	4 cut(s)	1966, 2759, 3489, 3929
BslFI	GGGAC	3 cut(s)	3518, 4163, 4217
BslI	CCNNNNNNNGG	9 cut(s)	823, 1217, 1324, 1589, 2432, 2460, 3615, 3755, 3935
BsmBI	CGTCTC	1 cut(s)	734
BsmFI	GGGAC	3 cut(s)	3518, 4163, 4217
BsmI	GAATGC	2 cut(s)	1791, 4201
BsnI	GGCC	5 cut(s)	528, 1111, 1671, 2338, 4167
Bso31I	GGTCTC	1 cut(s)	3098
BsoBI	CYCGRG	2 cut(s)	2126, 4168
Bsp1286I	GDGCHC	6 cut(s)	80, 942, 1025, 1153, 1452, 4175
Bsp13I	TCCGGA	1 cut(s)	2758
Bsp1407I	TGTACA	1 cut(s)	714
Bsp1720I	GCTNAGC	1 cut(s)	2319
Bsp19I	CCATGG	3 cut(s)	1343, 2454, 4130
BspANI	GGCC	5 cut(s)	528, 1111, 1671, 2338, 4167
BspDI	ATCGAT	1 cut(s)	3854
BspEI	TCCGGA	1 cut(s)	2758
BspFNI	CGCG	4 cut(s)	276, 299, 305, 3558
BspHI	TCATGA	2 cut(s)	691, 1795
BspMAI	CTGCAG	1 cut(s)	2071
BspMI	ACCTGC	4 cut(s)	256, 1627, 2116, 3810
BspOI	GCTAGC	1 cut(s)	2468
BspPI	GGATC	8 cut(s)	887, 1339, 2124, 2438, 2581, 2769, 2842, 3427
BspT107I	GGYRCC	3 cut(s)	627, 3970, 4134
BspTNI	GGTCTC	1 cut(s)	3098
BsrBI	CCGCTC	1 cut(s)	1874
BsrDI	GCAATG	9 cut(s)	690, 1828, 2175, 2339, 2586, 2628, 2934, 3457, 3712
BsrFI	RCCGGY	1 cut(s)	3488
BsrGI	TGTACA	1 cut(s)	714
BsrI	ACTGG	8 cut(s)	205, 956, 2546, 2847, 2988, 3273, 3280, 4211
BssAI	RCCGGY	1 cut(s)	3488
BssECI	CCNNGG	7 cut(s)	1343, 2454, 3500, 3556, 3782, 4130, 4175
BssSI	CACGAG	2 cut(s)	21, 3095
BssT1I	CCWWGG	4 cut(s)	1343, 2454, 3500, 4130
Bst2BI	CACGAG	2 cut(s)	21, 3095
Bst2UI	CCWGG	7 cut(s)	818, 1122, 1218, 2211, 2700, 3122, 3744
Bst6I	CTCTTC	1 cut(s)	1264
BstAPI	GCANNNNNTGC	4 cut(s)	23, 850, 2372, 2672
BstAUI	TGTACA	1 cut(s)	714
BstBAI	YACGTR	1 cut(s)	1154
BstC8I	GCNNGC	6 cut(s)	278, 938, 2355, 2438, 2466, 4165
BstDSI	CCRYGG	5 cut(s)	1343, 2454, 3556, 4130, 4175
BstEII	GGTNACC	1 cut(s)	1313
BstFNI	CGCG	4 cut(s)	276, 299, 305, 3558
BstH2I	RGCGCY	1 cut(s)	803
BstHHI	GCGC	3 cut(s)	299, 802, 3904
BstNI	CCWGG	7 cut(s)	818, 1122, 1218, 2211, 2700, 3122, 3744
BstNSI	RCATGY	5 cut(s)	588, 1262, 2409, 2801, 3262
BstPI	GGTNACC	1 cut(s)	1313
BstSCI	CCNGG	9 cut(s)	816, 1120, 1216, 1965, 2209, 2698, 3120, 3742, 3927
BstSFI	CTRYAG	3 cut(s)	360, 1391, 2067
BstSLI	GKGCMC	1 cut(s)	1153
BstUI	CGCG	4 cut(s)	276, 299, 305, 3558
BstV2I	GAAGAC	3 cut(s)	1356, 2162, 3273
BstX2I	RGATCY	5 cut(s)	1331, 2116, 2389, 2761, 2834
BstXI	CCANNNNNNTGG	1 cut(s)	4146
BstYI	RGATCY	5 cut(s)	1331, 2116, 2389, 2761, 2834
Bsu15I	ATCGAT	1 cut(s)	3854
BsuRI	GGCC	5 cut(s)	528, 1111, 1671, 2338, 4167
BsuTUI	ATCGAT	1 cut(s)	3854
BtgI	CCRYGG	5 cut(s)	1343, 2454, 3556, 4130, 4175
BtgZI	GCGATG	3 cut(s)	74, 2627, 4100
BtrI	CACGTC	1 cut(s)	3508
BtsI	GCAGTG	3 cut(s)	1173, 2508, 3435
BveI	ACCTGC	4 cut(s)	256, 1627, 2116, 3810
Cac8I	GCNNGC	6 cut(s)	278, 938, 2355, 2438, 2466, 4165
CaiI	CAGNNNCTG	2 cut(s)	1235, 3518
CciI	TCATGA	2 cut(s)	691, 1795
CfoI	GCGC	3 cut(s)	299, 802, 3904
Cfr10I	RCCGGY	1 cut(s)	3488
Cfr13I	GGNCC	7 cut(s)	527, 555, 1616, 2336, 3746, 3946, 4166
Cfr42I	CCGCGG	1 cut(s)	3559
ClaI	ATCGAT	1 cut(s)	3854
CseI	GACGC	2 cut(s)	1415, 3134
Csp6I	GTAC	8 cut(s)	465, 628, 715, 1738, 2046, 3882, 4015, 4135
CspCI	CAANNNNNGTGG	2 cut(s)	2271, 2306
CviQI	GTAC	8 cut(s)	465, 628, 715, 1738, 2046, 3882, 4015, 4135
DraIII	CACNNNGTG	1 cut(s)	1853
DrdI	GACNNNNNNGTC	1 cut(s)	3938
DseDI	GACNNNNNNGTC	1 cut(s)	3938
EaeI	YGGCCR	1 cut(s)	1669
Eam1104I	CTCTTC	1 cut(s)	1264
EarI	CTCTTC	1 cut(s)	1264
Ecl136II	GAGCTC	1 cut(s)	78
Eco130I	CCWWGG	4 cut(s)	1343, 2454, 3500, 4130
Eco24I	GRGCYC	3 cut(s)	80, 1452, 4175
Eco31I	GGTCTC	1 cut(s)	3098
Eco32I	GATATC	1 cut(s)	919
Eco47I	GGWCC	4 cut(s)	555, 1616, 3746, 3946
Eco53kI	GAGCTC	1 cut(s)	78
Eco57I	CTGAAG	3 cut(s)	2628, 3102, 4029
Eco72I	CACGTG	1 cut(s)	1154
Eco88I	CYCGRG	2 cut(s)	2126, 4168
Eco91I	GGTNACC	1 cut(s)	1313
EcoICRI	GAGCTC	1 cut(s)	78
EcoO109I	RGGNCCY	1 cut(s)	3746
EcoO65I	GGTNACC	1 cut(s)	1313
EcoRII	CCWGG	7 cut(s)	816, 1120, 1216, 2209, 2698, 3120, 3742
EcoRV	GATATC	1 cut(s)	919
EcoT14I	CCWWGG	4 cut(s)	1343, 2454, 3500, 4130
EcoT38I	GRGCYC	3 cut(s)	80, 1452, 4175
ErhI	CCWWGG	4 cut(s)	1343, 2454, 3500, 4130
Esp3I	CGTCTC	1 cut(s)	734
FaqI	GGGAC	3 cut(s)	3518, 4163, 4217
FauI	CCCGC	1 cut(s)	3752
FauNDI	CATATG	2 cut(s)	445, 3951
FbaI	TGATCA	4 cut(s)	1555, 1798, 1888, 3547
FblI	GTMKAC	1 cut(s)	946
FriOI	GRGCYC	3 cut(s)	80, 1452, 4175
FspBI	CTAG	9 cut(s)	864, 1074, 1379, 1410, 1470, 2460, 2465, 2532, 3527
GlaI	GCGC	3 cut(s)	298, 801, 3903
GsaI	CCCAGC	2 cut(s)	966, 4150
GsuI	CTGGAG	3 cut(s)	1257, 2682, 3552
HaeII	RGCGCY	1 cut(s)	803
HaeIII	GGCC	5 cut(s)	528, 1111, 1671, 2338, 4167
HapII	CCGG	4 cut(s)	1966, 2759, 3489, 3929
HgaI	GACGC	2 cut(s)	1415, 3134
HhaI	GCGC	3 cut(s)	299, 802, 3904
Hin6I	GCGC	3 cut(s)	297, 800, 3902
HinP1I	GCGC	3 cut(s)	297, 800, 3902
HincII	GTYRAC	1 cut(s)	947
HindII	GTYRAC	1 cut(s)	947
HindIII	AAGCTT	2 cut(s)	232, 2558
HpaII	CCGG	4 cut(s)	1966, 2759, 3489, 3929
Hpy99I	CGWCG	1 cut(s)	1409
HpyCH4IV	ACGT	9 cut(s)	1153, 1708, 2159, 2910, 3507, 3686, 4002, 4105, 4152
HpySE526I	ACGT	9 cut(s)	1153, 1708, 2159, 2910, 3507, 3686, 4002, 4105, 4152
HspAI	GCGC	3 cut(s)	297, 800, 3902
Kpn2I	TCCGGA	1 cut(s)	2758
KpnI	GGTACC	2 cut(s)	631, 4138
Ksp22I	TGATCA	4 cut(s)	1555, 1798, 1888, 3547
KspI	CCGCGG	1 cut(s)	3559
MaeI	CTAG	9 cut(s)	864, 1074, 1379, 1410, 1470, 2460, 2465, 2532, 3527
MaeII	ACGT	9 cut(s)	1153, 1708, 2159, 2910, 3507, 3686, 4002, 4105, 4152
MbiI	CCGCTC	1 cut(s)	1874
MfeI	CAATTG	2 cut(s)	89, 2634
MflI	RGATCY	5 cut(s)	1331, 2116, 2389, 2761, 2834
MhlI	GDGCHC	6 cut(s)	80, 942, 1025, 1153, 1452, 4175
MlsI	TGGCCA	1 cut(s)	1671
MluNI	TGGCCA	1 cut(s)	1671
MmeI	TCCRAC	5 cut(s)	773, 954, 1887, 2452, 3264
Mox20I	TGGCCA	1 cut(s)	1671
MroI	TCCGGA	1 cut(s)	2758
MroXI	GAANNNNTTC	3 cut(s)	1045, 1273, 4187
MscI	TGGCCA	1 cut(s)	1671
MslI	CAYNNNNRTG	3 cut(s)	1634, 3231, 3830
Msp20I	TGGCCA	1 cut(s)	1671
MspA1I	CMGCKG	5 cut(s)	73, 191, 2066, 3473, 3558
MspI	CCGG	4 cut(s)	1966, 2759, 3489, 3929
MspR9I	CCNGG	9 cut(s)	818, 1122, 1218, 1967, 2211, 2700, 3122, 3744, 3929
MunI	CAATTG	2 cut(s)	89, 2634
Mva1269I	GAATGC	2 cut(s)	1791, 4201
MvaI	CCWGG	7 cut(s)	818, 1122, 1218, 2211, 2700, 3122, 3744
MvnI	CGCG	4 cut(s)	276, 299, 305, 3558
NciI	CCSGG	2 cut(s)	1967, 3929
NcoI	CCATGG	3 cut(s)	1343, 2454, 4130
NdeI	CATATG	2 cut(s)	445, 3951
NheI	GCTAGC	1 cut(s)	2464
NmeAIII	GCCGAG	1 cut(s)	3807
NmuCI	GTSAC	9 cut(s)	601, 2302, 2506, 2752, 3181, 3508, 3762, 3832, 4003
NspI	RCATGY	5 cut(s)	588, 1262, 2409, 2801, 3262
OliI	CACNNNNGTG	1 cut(s)	3830
PaeR7I	CTCGAG	1 cut(s)	2126
PagI	TCATGA	2 cut(s)	691, 1795
PaqCI	CACCTGC	1 cut(s)	2116
PciI	ACATGT	1 cut(s)	3258
PctI	GAATGC	2 cut(s)	1791, 4201
PdmI	GAANNNNTTC	3 cut(s)	1045, 1273, 4187
PflFI	GACNNNGTC	1 cut(s)	3514
PflMI	CCANNNNNTGG	3 cut(s)	823, 1589, 3615
PfoI	TCCNGGA	3 cut(s)	2209, 2698, 3927
PmaCI	CACGTG	1 cut(s)	1154
PmlI	CACGTG	1 cut(s)	1154
Ppu21I	YACGTR	1 cut(s)	1154
PpuMI	RGGWCCY	1 cut(s)	3746
PscI	ACATGT	1 cut(s)	3258
PshBI	ATTAAT	1 cut(s)	2028
Psp124BI	GAGCTC	1 cut(s)	80
Psp1406I	AACGTT	2 cut(s)	2910, 4105
Psp5II	RGGWCCY	1 cut(s)	3746
Psp6I	CCWGG	7 cut(s)	816, 1120, 1216, 2209, 2698, 3120, 3742
PspCI	CACGTG	1 cut(s)	1154
PspEI	GGTNACC	1 cut(s)	1313
PspFI	CCCAGC	2 cut(s)	962, 4146
PspGI	CCWGG	7 cut(s)	816, 1120, 1216, 2209, 2698, 3120, 3742
PspPI	GGNCC	7 cut(s)	527, 555, 1616, 2336, 3746, 3946, 4166
PspPPI	RGGWCCY	1 cut(s)	3746
PsrI	GAACNNNNNNTAC	2 cut(s)	620, 652
PstI	CTGCAG	1 cut(s)	2071
PstNI	CAGNNNCTG	2 cut(s)	1235, 3518
PsuI	RGATCY	5 cut(s)	1331, 2116, 2389, 2761, 2834
PsyI	GACNNNGTC	1 cut(s)	3514
PvuII	CAGCTG	3 cut(s)	191, 2066, 3473
RsaI	GTAC	8 cut(s)	466, 629, 716, 1739, 2047, 3883, 4016, 4136
RsaNI	GTAC	8 cut(s)	465, 628, 715, 1738, 2046, 3882, 4015, 4135
RseI	CAYNNNNRTG	3 cut(s)	1634, 3231, 3830
SacI	GAGCTC	1 cut(s)	80
SacII	CCGCGG	1 cut(s)	3559
SalI	GTCGAC	1 cut(s)	945
Sau96I	GGNCC	7 cut(s)	527, 555, 1616, 2336, 3746, 3946, 4166
ScaI	AGTACT	1 cut(s)	3883
ScrFI	CCNGG	9 cut(s)	818, 1122, 1218, 1967, 2211, 2700, 3122, 3744, 3929
SduI	GDGCHC	6 cut(s)	80, 942, 1025, 1153, 1452, 4175
SfcI	CTRYAG	3 cut(s)	360, 1391, 2067
Sfr274I	CTCGAG	1 cut(s)	2126
Sfr303I	CCGCGG	1 cut(s)	3559
SgrBI	CCGCGG	1 cut(s)	3559
SinI	GGWCC	4 cut(s)	555, 1616, 3746, 3946
SlaI	CTCGAG	1 cut(s)	2126
SmiMI	CAYNNNNRTG	3 cut(s)	1634, 3231, 3830
SmlI	CTYRAG	6 cut(s)	154, 314, 479, 2126, 3101, 3750
SmoI	CTYRAG	6 cut(s)	154, 314, 479, 2126, 3101, 3750
SspI	AATATT	1 cut(s)	379
SspMI	CTAG	9 cut(s)	864, 1074, 1379, 1410, 1470, 2460, 2465, 2532, 3527
SstI	GAGCTC	1 cut(s)	80
StyD4I	CCNGG	9 cut(s)	816, 1120, 1216, 1965, 2209, 2698, 3120, 3742, 3927
StyI	CCWWGG	4 cut(s)	1343, 2454, 3500, 4130
TaiI	ACGT	9 cut(s)	1156, 1711, 2162, 2913, 3510, 3689, 4005, 4108, 4155
TaqII	GACCGA	1 cut(s)	719
TatI	WGTACW	3 cut(s)	714, 2045, 3881
TauI	GCSGC	3 cut(s)	29, 3902, 4055
TseFI	GTSAC	9 cut(s)	601, 2302, 2506, 2752, 3181, 3508, 3762, 3832, 4003
Tsp45I	GTSAC	9 cut(s)	601, 2302, 2506, 2752, 3181, 3508, 3762, 3832, 4003
TspDTI	ATGAA	6 cut(s)	17, 420, 680, 723, 1784, 3234
TspGWI	ACGGA	3 cut(s)	482, 3134, 3921
Tth111I	GACNNNGTC	1 cut(s)	3514
Van91I	CCANNNNNTGG	3 cut(s)	823, 1589, 3615
VneI	GTGCAC	1 cut(s)	1149
VpaK11BI	GGWCC	4 cut(s)	555, 1616, 3746, 3946
VspI	ATTAAT	1 cut(s)	2028
XapI	RAATTY	3 cut(s)	457, 674, 1439
XceI	RCATGY	5 cut(s)	588, 1262, 2409, 2801, 3262
XcmI	CCANNNNNNNNNTGG	1 cut(s)	3280
XhoI	CTCGAG	1 cut(s)	2126
XmiI	GTMKAC	1 cut(s)	946
XmnI	GAANNNNTTC	3 cut(s)	1045, 1273, 4187
XspI	CTAG	9 cut(s)	864, 1074, 1379, 1410, 1470, 2460, 2465, 2532, 3527
ZrmI	AGTACT	1 cut(s)	3883

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.