Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr4

Orthogroup ID

OG0005762

Physical Location & Seq

Genomic Coordinates Reverse (-)

1714086 .. 1717613

Gene Structure 3528 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom04g01930.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3528 bp

ATGACTGAGTGTGTGTATGCCCAGAAGCTAGTTTCATTTGAAACGAGTGTAGTTCCGGATGAAGTTCGTCATGTACATGTCATGGAGAGGGGTCGGAAGGCATTGGAGGAGATAAATCAGGAAATGGGTTTGGCATTTGATGAGCAGGATCTCCAGTACTACACTAGGCTATTCAAGGATGAAATCCAGAGGAATCCAACAACAGTTGAGCTGTTTGATATTGCACAATCCAACAGTGAGCATAGTAGGCACTGGTTTTTTACTGGCAAAATTATTATAGATGGACAGCCTATGGATAGAACTCTGATGCAGATTGTGAAGAGCACGTTGCAGGCAAACCCAAACAATTCTGTAATTGGCTTCAAGGATAACTCAAGTGCAATCAAGGGGTTTCTTGTAAAGCAAATGCGACCGGTTGAGCCAGGTTCAACATGTCCATTGAGCATAGCTACTCGGTACCTTGATATTTTATTTACTGCTGAGACCCATAATTTCCCATGTGCTGTAGCGCCTTACCCTGGTGCGGAGACAGGCGCAGGAGGTCGAATCAGGGATACCCATGCGACTGGAAGAGGGTCTTTTGTTGTAGCATCTACAGCTGGTTATTGTGTTGGGAATCTCAATATGGAGGGCTCTTATGCACCTTGGGAAGATCCGTCTTTTGCTTATCCGTCAAATTTGGCTCCACCTCTGCAGATCCTTATAGATGCTAGCAATGGTGCATCAGACTACGGGAACAAATTTGGTGAGCCCTTGATTCAGGGCTACACTAGAACATTTGGAATGAGACTTCCAAGTGGAGACAGGAGAGAATGGTTGAAGCCAATCATGTTTAGTGGAGGCATTGGGCAGATTGATCACACCCATATAACAAAAGGTGAGCCTGACATTGGTATGCTGGTTGTTAAAATTGGAGGCCCTGCTTATCGTATTGGAATGGGGGGTGGGGCAGCATCGAGCATGGTTAGCGGCCAGAATGATGCGGAGCTTGATTTTAATGCTGTACAGCGTGGAGATGCAGAGATGGCACAGAAACTTTATCGTGTGGTCCGTGCCTGTATTGAGATGGGGGATAATAACCCTATCATTAGCATTCATGATCAGGGGGCAGGTGGAAACTGCAATGTTGTTAAGGAAATTATATACCCCAAGGGCGGCCAGATTGATATCCGGGCAATTGTAGTTGGTGATCACACAATGTCTGTCTTGGAGATATGGGGTGCAGAATACCAGGAGCAAGATGCAATCTTGGTGAAGCCTGAAAGCCGCCACCTCTTGCAATCAATATGTGAAAGAGAAAGGGTTTCTATGGCTGTTATTGGAACAATAAATGGTGAGGGACGTGCTGTTTTAATTGATAGCTTAGCTATTAAAAAATGTGAATCTAGTGGACTCCCTCCCCCTCCTCCTGCAGTCGATTTGGAGCTTGAGAAAGTACTTGGTGATATGCCACAGAAAAGTTTTGAATTCCACAGGACGATTGATGCACGAGAACCACTTGATATTGCTCCTGGAATAACAGTGATGGATTCACTTAAGAGGGTGTTGAGACTTCCATCTGTTTGTTCAAAGCGCTTCTTGACATCCAAAGTAGACAGGTGTGTTACAGGTCTCGTAGCACAGCAGCAAACTGTTGGGCCCTTGCAAATTCCTCTCTCTGATGTTGCAGTAATAGCGCAGACTTTTACTGATGTGACTGGAGGTGCATGTGCCATTGGGGAGCAGCCAATCAAAGGTTTGCTGGATCCAAAAGCAATGGCGAGATTGGCTGTGGGAGAAGCACTCACAAATCTTGTTTGGGCAAAGGTCACTTCTCTATCTGATGTTAAAGCCAGCGGGAATTGGATGTATGCAGCCAAGCTTGATGGGGAAGGAGCAGCTATGTATGATGCTGCTACAGCTCTTTCAGAGGCAATGATCGAACTTGGTATTGCAATTGATGGCGGGAAGGACAGTCTTTCCATGGCAGCCCATGTTGCAGGCGAGGTTGTTAAGGCACCTGGCAATCTTGTAATGAGTGTTTATTGCACTTGTCCTGACATAACAAAAACAGTAACCCCAGATTTAAAGCTCAAGGATGATGGCGTGCTGCTTCACATTGATTTGGCAAAGGGAAAGCGGAGGTTAGGTGGGTCTGCTCTTGCTCAGGTTTTTGACCAAGTTGGCAATGACTGCCCTGACATTGAGGATGTTCCTTATCTAAAACGAGTTTTTGAGGGAGTTCAGGACCTTCTTTCTGACGAATTGATCTCTGCTGGTCATGATATTAGTGATGGTGGGCTACTGGTCTGTGCCCTTGAAATGGCATTTTCTGGGAATTGTGGCATAACTTTGGACTTGACTTCACATGGGAAGGGCCTATTCCAAACGCTTTTTGCTGAAGAACTTGGTCTGGTTATTGAGGTAAGCCGGAATGACTTAGATTTGGTGTTGGAAAAACTGAGCAGTTACAATATTTTGGCTGAGAATATTGGTCAAGTAAGTGCCACACCCTCAGTAGAGTTAAAGGTTGATGGGGTTACACATTTGAATGAGAGTACTTCTTTTCTAAGGGACTTGTGGGAGGACACTAGTTTTCAGCTGGAAAGACTTCAAAGGTTGGCTTCTTGTGTGGATCTAGAAAAAGAAGGGTTGAAAGATAGGCATGAGCCCTCATGGGATTTGTCTTTCACTCCTTCTTTCACAGATGAAAAGTATATGACCATCGCTTGCAAACCAAAAGTGGCTATAATCCGAGAAGAAGGCAGCAATGGGGACAGAGAAATGTCGGCAGCATTTTATGCTTCTGGTTTTGAACCATGGGATGTTACAATGTCAGACCTTCTTAATGGAACCATTTCATTGCATGAATTCCGTGGAATTGCGTTTGTTGGAGGTTTTAGCTACGCCGATGTGCTTGATTCTGCAAAAGGTTGGTCTGCATCGATTCGATTTAATCAGCCCCTTCTAAATCAATTTCAAGAGTTTTACAAGCGGCCAGACACTTTTAGTCTTGGAGTTTGCAACGGGTGTCAGCTTATGGCCCTTTTGGGGTGGGTCCCGGGTCCTCAAGTTGGTGGTGTGCTAGGTGGTGGTGGGGACCCTTCGCAGCCCAGGTTCATTCACAACGAGTCTGGACGGTTTGAATGCCGATTCACCAGTGTGGCAATAAAGGACTCGCCAGCTATAATGTTTAAAGGAATGGAGGGCAGTACATTGGGTGTGTGGGCTGCACATGGTGAAGGAAGAGCATATTTCCCCGATGATGGTGTTCTTGATCGTTTGCTTCACTCAAAGTTGGCACCAGTAAGGTATTGCGATGATGATGGTAATGAGACAGAAGTTTACCCTTTCAACGTCAATGGCTCTCCCTTGGGGGTTGCAGCAATTTGTTCTCCAGATGGGAGGCATCTAGCCATGATGCCTCATCCAGAGCGTTGCTTCTTAATGTGGCAATTCCCCTGGTATCCCAAGCAATGGGATGTGGAAAAGAAAGGCCCTAGTCCCTGGTTGCGAATGTTCCAGAATGCTAGAGAGTGGTGTTCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0003674 GO:0003824 GO:0004642 GO:0005488 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0007275 GO:0008144 GO:0008150 GO:0009507 GO:0009532 GO:0009536 GO:0009555 GO:0009570 GO:0016874 GO:0016879 GO:0016884 GO:0017076 GO:0030554 GO:0032501 GO:0032502 GO:0032553 GO:0032555 GO:0032559 GO:0035639 GO:0036094 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0048229 GO:0048856 GO:0055046 GO:0097159 GO:0097367 GO:1901265 GO:1901363

KEGG Pathways

Metabolic & Signaling

ko00230 ko01100 ko01110 ko01130 map00230 map01100 map01110 map01130

Pfam Domains

Protein Families

AIRS_C GATase_5

Protein Analysis

1176

Amino Acids

128.2

Weight (kDa)

5.03

Isoelectric Point (pI)

36.22

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FGAR-AT_linker	PF18072	35 - 84	7.3e-11	Formylglycinamide ribonucleotide amidotransferase linker domain
AIRS_C	PF02769	298 - 452	3.1e-26	AIR synthase related protein, C-terminal domain
FGAR-AT_PurM_N-like	PF22689	523 - 676	1.3e-52	FGAR-AT PurM_N-like domain
AIRS_C	PF02769	707 - 837	1e-17	AIR synthase related protein, C-terminal domain
GATase_5	PF13507	905 - 1174	1.1e-105	CobB/CobQ-like glutamine amidotransferase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0005762)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G74260
fragaria_vesca	FvH4_5g19530 FvH4_5g19530 FvH4_5g19530
malus_domestica	MD03G1203100.v1.1 MD04G1022300.v1.1
prunus_persica	Prupe.1G542100_v2.0.a1 Prupe.1G542100_v2.0.a1 Prupe.1G542100_v2.0.a1 Prupe.1G542100_v2.0.a1 Prupe.1G542100_v2.0.a1 Prupe.8G024000_v2.0.a1 Prupe.8G024000_v2.0.a1 Prupe.8G024000_v2.0.a1
pyrus_communis	pycom04g01890 pycom04g01930
rosa_chinensis	RchiOBHm_Chr7g0204141
rosa_laevigata	RLG00000003485
rosa_multiflora	Rmu_sc0001311.1_g000031
rosa_roxburghii	Rroxscaffold_3G00253310
rosa_rugosa	Rorug07G0081100 Rorug07G0081200 Rorug07G0081200
rosa_samantha	Rh7AG211000 Rh7BG209600 Rh7CG223500 Rh7DG217800
rosa_wichuraiana	Rw7G018360

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1100
Acc36I	ACCTGC	1 cut(s)	1100
Acc65I	GGTACC	1 cut(s)	456
AccB1I	GGYRCC	3 cut(s)	456, 1996, 3280
AccB7I	CCANNNNNTGG	1 cut(s)	3456
AccI	GTMKAC	1 cut(s)	1593
AccIII	TCCGGA	1 cut(s)	55
AciI	CCGC	9 cut(s)	524, 969, 983, 1155, 1267, 1836, 1944, 2119, 2974
AclWI	GGATC	6 cut(s)	156, 647, 691, 1739, 1752, 2622
AcoI	YGGCCR	3 cut(s)	970, 1156, 2975
AcsI	RAATTY	5 cut(s)	676, 740, 1466, 1647, 2849
AcuI	CTGAAG	1 cut(s)	2400
AdeI	CACNNNGTG	1 cut(s)	3218
AfaI	GTAC	7 cut(s)	75, 158, 458, 1005, 1437, 2539, 3193
AfeI	AGCGCT	1 cut(s)	1574
AflII	CTTAAG	1 cut(s)	1535
AflIII	ACRYGT	2 cut(s)	76, 431
AgeI	ACCGGT	1 cut(s)	412
AhlI	ACTAGT	1 cut(s)	2570
AjiI	CACGTC	1 cut(s)	1343
AjnI	CCWGG	8 cut(s)	421, 517, 1230, 1510, 1999, 3092, 3440, 3485
AleI	CACNNNNGTG	1 cut(s)	3140
Alw21I	GWGCWC	1 cut(s)	326
Alw26I	GTCTC	7 cut(s)	476, 521, 781, 795, 1543, 1616, 3308
AlwI	GGATC	6 cut(s)	156, 647, 691, 1739, 1752, 2622
Ama87I	CYCGRG	1 cut(s)	3040
Aor13HI	TCCGGA	1 cut(s)	55
Aor51HI	AGCGCT	1 cut(s)	1574
AoxI	GGCC	8 cut(s)	916, 970, 1156, 1637, 2356, 2975, 3021, 3475
ApaI	GGGCCC	1 cut(s)	1641
ApoI	RAATTY	5 cut(s)	676, 740, 1466, 1647, 2849
ArsI	GACNNNNNNTTYG	2 cut(s)	2900, 2932
AsiGI	ACCGGT	1 cut(s)	412
Asp700I	GAANNNNTTC	2 cut(s)	2589, 2836
Asp718I	GGTACC	1 cut(s)	456
AspLEI	GCGC	4 cut(s)	511, 536, 1575, 1678
AsuC2I	CCSGG	3 cut(s)	1172, 3041, 3042
AsuHPI	GGTGA	8 cut(s)	758, 890, 1199, 1264, 1346, 1454, 3127, 3230
AsuNHI	GCTAGC	1 cut(s)	710
AvaI	CYCGRG	1 cut(s)	3040
AvaII	GGWCC	5 cut(s)	1048, 2227, 3037, 3044, 3079
BaeGI	GKGCMC	2 cut(s)	1641, 2296
BamHI	GGATCC	1 cut(s)	1744
BanI	GGYRCC	3 cut(s)	456, 1996, 3280
BanII	GRGCYC	4 cut(s)	635, 753, 1641, 2652
BauI	CACGAG	1 cut(s)	1488
Bbv12I	GWGCWC	1 cut(s)	326
BciT130I	CCWGG	8 cut(s)	423, 519, 1232, 1512, 2001, 3094, 3442, 3487
BciVI	GTATCC	2 cut(s)	547, 3456
BclI	TGATCA	3 cut(s)	856, 1099, 1189
BcnI	CCSGG	3 cut(s)	1172, 3041, 3042
BcoDI	GTCTC	7 cut(s)	476, 521, 781, 795, 1543, 1616, 3308
BcuI	ACTAGT	1 cut(s)	2570
BfmI	CTRYAG	5 cut(s)	504, 594, 692, 1410, 1896
BfoI	RGCGCY	2 cut(s)	512, 1576
BfrI	CTTAAG	1 cut(s)	1535
BfuAI	ACCTGC	1 cut(s)	1100
BfuI	GTATCC	2 cut(s)	547, 3456
BlpI	GCTNAGC	1 cut(s)	1363
BmcAI	AGTACT	3 cut(s)	158, 1437, 2539
Bme18I	GGWCC	5 cut(s)	1048, 2227, 3037, 3044, 3079
BmeT110I	CYCGRG	1 cut(s)	3040
BmgBI	CACGTC	1 cut(s)	1343
BmtI	GCTAGC	1 cut(s)	714
BplI	GAGNNNNNCTC	2 cut(s)	1767, 1799
BpmI	CTGGAG	3 cut(s)	137, 1719, 3360
Bpu10I	CCTNAGC	1 cut(s)	2145
Bpu1102I	GCTNAGC	1 cut(s)	1363
BpuEI	CTTGAG	4 cut(s)	358, 1448, 2057, 3033
BpuMI	CCSGG	3 cut(s)	1172, 3041, 3042
Bsa29I	ATCGAT	1 cut(s)	2924
BsaBI	GATNNNNATC	1 cut(s)	1166
BsaI	GGTCTC	2 cut(s)	476, 1616
BsaWI	WCCGGW	2 cut(s)	55, 412
BsaXI	ACNNNNNCTCC	2 cut(s)	2113, 2143
Bse118I	RCCGGY	1 cut(s)	412
Bse1I	ACTGG	8 cut(s)	154, 257, 268, 571, 1702, 2289, 3138, 3284
Bse3DI	GCAATG	8 cut(s)	721, 1129, 1761, 1920, 2173, 2755, 2840, 3461
Bse8I	GATNNNNATC	1 cut(s)	1166
BseAI	TCCGGA	1 cut(s)	55
BseBI	CCWGG	8 cut(s)	423, 519, 1232, 1512, 2001, 3094, 3442, 3487
BseCI	ATCGAT	1 cut(s)	2924
BseGI	GGATG	9 cut(s)	64, 184, 1583, 1851, 2083, 2194, 2809, 3406, 3466
BseJI	GATNNNNATC	1 cut(s)	1166
BseMI	GCAATG	8 cut(s)	721, 1129, 1761, 1920, 2173, 2755, 2840, 3461
BseMII	CTCAG	5 cut(s)	471, 2159, 2432, 2454, 2508
BseNI	ACTGG	8 cut(s)	154, 257, 268, 571, 1702, 2289, 3138, 3284
BseRI	GAGGAG	2 cut(s)	122, 1395
BseSI	GKGCMC	2 cut(s)	1641, 2296
BsgI	GTGCAG	2 cut(s)	1242, 3195
Bsh1285I	CGRYCG	1 cut(s)	413
BshFI	GGCC	8 cut(s)	918, 972, 1158, 1639, 2358, 2977, 3023, 3477
BshNI	GGYRCC	3 cut(s)	456, 1996, 3280
BshTI	ACCGGT	1 cut(s)	412
BshVI	ATCGAT	1 cut(s)	2924
BsiEI	CGRYCG	1 cut(s)	413
BsiHKAI	GWGCWC	1 cut(s)	326
BsiHKCI	CYCGRG	1 cut(s)	3040
BsiSI	CCGG	5 cut(s)	56, 413, 1171, 2410, 3041
BslFI	GGGAC	6 cut(s)	1353, 2567, 2768, 3023, 3092, 3468
BsmAI	GTCTC	7 cut(s)	476, 521, 781, 795, 1543, 1616, 3308
BsmFI	GGGAC	6 cut(s)	1353, 2567, 2768, 3023, 3092, 3468
BsmI	GAATGC	3 cut(s)	1092, 3131, 3511
BsnI	GGCC	8 cut(s)	918, 972, 1158, 1639, 2358, 2977, 3023, 3477
Bso31I	GGTCTC	2 cut(s)	476, 1616
BsoBI	CYCGRG	1 cut(s)	3040
Bsp120I	GGGCCC	1 cut(s)	1637
Bsp1286I	GDGCHC	6 cut(s)	326, 635, 753, 1641, 2296, 2652
Bsp13I	TCCGGA	1 cut(s)	55
Bsp1407I	TGTACA	2 cut(s)	73, 1003
Bsp1720I	GCTNAGC	1 cut(s)	1363
Bsp19I	CCATGG	2 cut(s)	1962, 2798
BspACI	CCGC	9 cut(s)	524, 969, 983, 1155, 1267, 1836, 1944, 2119, 2974
BspANI	GGCC	8 cut(s)	918, 972, 1158, 1639, 2358, 2977, 3023, 3477
BspCNI	CTCAG	5 cut(s)	472, 2158, 2433, 2455, 2507
BspDI	ATCGAT	1 cut(s)	2924
BspEI	TCCGGA	1 cut(s)	55
BspHI	TCATGA	2 cut(s)	1096, 2260
BspMAI	CTGCAG	2 cut(s)	696, 1414
BspMI	ACCTGC	1 cut(s)	1100
BspOI	GCTAGC	1 cut(s)	714
BspPI	GGATC	6 cut(s)	156, 647, 691, 1739, 1752, 2622
BspQI	GCTCTTC	2 cut(s)	314, 3220
BspT107I	GGYRCC	3 cut(s)	456, 1996, 3280
BspTI	CTTAAG	1 cut(s)	1535
BspTNI	GGTCTC	2 cut(s)	476, 1616
BsrDI	GCAATG	8 cut(s)	721, 1129, 1761, 1920, 2173, 2755, 2840, 3461
BsrFI	RCCGGY	1 cut(s)	412
BsrGI	TGTACA	2 cut(s)	73, 1003
BsrI	ACTGG	8 cut(s)	154, 257, 268, 571, 1702, 2289, 3138, 3284
BssAI	RCCGGY	1 cut(s)	412
BssSI	CACGAG	1 cut(s)	1488
BssT1I	CCWWGG	5 cut(s)	644, 1149, 1962, 2798, 3351
Bst2BI	CACGAG	1 cut(s)	1488
Bst2UI	CCWGG	8 cut(s)	423, 519, 1232, 1512, 2001, 3094, 3442, 3487
Bst4CI	ACNGT	7 cut(s)	205, 236, 1522, 1633, 1955, 2053, 3120
Bst6I	CTCTTC	3 cut(s)	314, 565, 3220
BstAFI	CTTAAG	1 cut(s)	1535
BstAPI	GCANNNNNTGC	2 cut(s)	2172, 2780
BstAUI	TGTACA	2 cut(s)	73, 1003
BstC8I	GCNNGC	7 cut(s)	333, 712, 1834, 1981, 2087, 2710, 3162
BstDEI	CTNAG	9 cut(s)	6, 480, 1363, 2145, 2419, 2441, 2463, 2494, 2549
BstDSI	CCRYGG	3 cut(s)	1962, 2798, 2854
BstF5I	GGATG	9 cut(s)	64, 184, 1583, 1851, 2083, 2194, 2809, 3406, 3466
BstH2I	RGCGCY	2 cut(s)	512, 1576
BstHHI	GCGC	4 cut(s)	511, 536, 1575, 1678
BstMAI	GTCTC	7 cut(s)	476, 521, 781, 795, 1543, 1616, 3308
BstMCI	CGRYCG	1 cut(s)	413
BstNI	CCWGG	8 cut(s)	423, 519, 1232, 1512, 2001, 3094, 3442, 3487
BstNSI	RCATGY	3 cut(s)	80, 435, 1710
BstSFI	CTRYAG	5 cut(s)	504, 594, 692, 1410, 1896
BstSLI	GKGCMC	2 cut(s)	1641, 2296
BstX2I	RGATCY	5 cut(s)	148, 652, 696, 1744, 2614
BstXI	CCANNNNNNTGG	1 cut(s)	566
BstYI	RGATCY	5 cut(s)	148, 652, 696, 1744, 2614
Bsu15I	ATCGAT	1 cut(s)	2924
BsuI	GTATCC	2 cut(s)	547, 3456
BsuRI	GGCC	8 cut(s)	918, 972, 1158, 1639, 2358, 2977, 3023, 3477
BsuTUI	ATCGAT	1 cut(s)	2924
BtgI	CCRYGG	3 cut(s)	1962, 2798, 2854
BtgZI	GCGATG	2 cut(s)	2689, 3312
BtrI	CACGTC	1 cut(s)	1343
BtsCI	GGATG	9 cut(s)	64, 184, 1583, 1851, 2083, 2194, 2809, 3406, 3466
BtsIMutI	CAGTG	4 cut(s)	241, 250, 1527, 3145
BveI	ACCTGC	1 cut(s)	1100
Cac8I	GCNNGC	7 cut(s)	333, 712, 1834, 1981, 2087, 2710, 3162
CciI	TCATGA	2 cut(s)	1096, 2260
CfoI	GCGC	4 cut(s)	511, 536, 1575, 1678
Cfr10I	RCCGGY	1 cut(s)	412
Cfr9I	CCCGGG	1 cut(s)	3040
ClaI	ATCGAT	1 cut(s)	2924
Csp6I	GTAC	7 cut(s)	74, 157, 457, 1004, 1436, 2538, 3192
CspAI	ACCGGT	1 cut(s)	412
CviQI	GTAC	7 cut(s)	74, 157, 457, 1004, 1436, 2538, 3192
DdeI	CTNAG	9 cut(s)	6, 480, 1363, 2145, 2419, 2441, 2463, 2494, 2549
DraI	TTTAAA	2 cut(s)	2067, 3175
DraIII	CACNNNGTG	1 cut(s)	3218
EaeI	YGGCCR	3 cut(s)	970, 1156, 2975
Eam1104I	CTCTTC	3 cut(s)	314, 565, 3220
EarI	CTCTTC	3 cut(s)	314, 565, 3220
Eco130I	CCWWGG	5 cut(s)	644, 1149, 1962, 2798, 3351
Eco24I	GRGCYC	4 cut(s)	635, 753, 1641, 2652
Eco31I	GGTCTC	2 cut(s)	476, 1616
Eco32I	GATATC	1 cut(s)	1168
Eco47I	GGWCC	5 cut(s)	1048, 2227, 3037, 3044, 3079
Eco47III	AGCGCT	1 cut(s)	1574
Eco57I	CTGAAG	1 cut(s)	2400
Eco88I	CYCGRG	1 cut(s)	3040
EcoO109I	RGGNCCY	8 cut(s)	917, 1638, 2227, 2356, 3037, 3044, 3079, 3476
EcoRI	GAATTC	2 cut(s)	1466, 2849
EcoRII	CCWGG	8 cut(s)	421, 517, 1230, 1510, 1999, 3092, 3440, 3485
EcoRV	GATATC	1 cut(s)	1168
EcoT14I	CCWWGG	5 cut(s)	644, 1149, 1962, 2798, 3351
EcoT38I	GRGCYC	4 cut(s)	635, 753, 1641, 2652
ErhI	CCWWGG	5 cut(s)	644, 1149, 1962, 2798, 3351
FalI	AAGNNNNNCTT	6 cut(s)	562, 594, 1562, 1594, 2587, 2619
FaqI	GGGAC	6 cut(s)	1353, 2567, 2768, 3023, 3092, 3468
FauI	CCCGC	2 cut(s)	1829, 1937
FbaI	TGATCA	3 cut(s)	856, 1099, 1189
FblI	GTMKAC	1 cut(s)	1593
FokI	GGATG	9 cut(s)	71, 191, 1570, 1858, 2090, 2201, 2816, 3393, 3473
FriOI	GRGCYC	4 cut(s)	635, 753, 1641, 2652
GlaI	GCGC	4 cut(s)	510, 535, 1574, 1677
GsuI	CTGGAG	3 cut(s)	137, 1719, 3360
HaeII	RGCGCY	2 cut(s)	512, 1576
HaeIII	GGCC	8 cut(s)	918, 972, 1158, 1639, 2358, 2977, 3023, 3477
HapII	CCGG	5 cut(s)	56, 413, 1171, 2410, 3041
HhaI	GCGC	4 cut(s)	511, 536, 1575, 1678
Hin6I	GCGC	4 cut(s)	509, 534, 1573, 1676
HinP1I	GCGC	4 cut(s)	509, 534, 1573, 1676
HindIII	AAGCTT	1 cut(s)	1859
HpaII	CCGG	5 cut(s)	56, 413, 1171, 2410, 3041
HphI	GGTGA	8 cut(s)	758, 890, 1199, 1264, 1346, 1454, 3127, 3230
Hpy166II	GTNNAC	3 cut(s)	1391, 1594, 3325
Hpy188I	TCNGA	9 cut(s)	96, 306, 727, 1660, 1822, 1909, 2239, 2735, 2818
Hpy8I	GTNNAC	3 cut(s)	1391, 1594, 3325
HpyCH4III	ACNGT	7 cut(s)	205, 236, 1522, 1633, 1955, 2053, 3120
HpyCH4IV	ACGT	3 cut(s)	326, 1342, 3336
HpyF3I	CTNAG	9 cut(s)	6, 480, 1363, 2145, 2419, 2441, 2463, 2494, 2549
HpySE526I	ACGT	3 cut(s)	326, 1342, 3336
HspAI	GCGC	4 cut(s)	509, 534, 1573, 1676
KflI	GGGWCCC	2 cut(s)	3037, 3079
Kpn2I	TCCGGA	1 cut(s)	55
KpnI	GGTACC	1 cut(s)	460
Ksp22I	TGATCA	3 cut(s)	856, 1099, 1189
LguI	GCTCTTC	2 cut(s)	314, 3220
LmnI	GCTCC	7 cut(s)	688, 985, 1234, 1423, 1513, 1720, 1874
MaeII	ACGT	3 cut(s)	326, 1342, 3336
MaeIII	GTNAC	7 cut(s)	1603, 1693, 1807, 2053, 2447, 2518, 2806
MboII	GAAGA	6 cut(s)	331, 582, 662, 2393, 2750, 3237
MfeI	CAATTG	2 cut(s)	1176, 1935
MflI	RGATCY	5 cut(s)	148, 652, 696, 1744, 2614
MhlI	GDGCHC	6 cut(s)	326, 635, 753, 1641, 2296, 2652
MlyI	GAGTC	3 cut(s)	1386, 3119, 3149
MmeI	TCCRAC	5 cut(s)	74, 221, 255, 2412, 2851
MroI	TCCGGA	1 cut(s)	55
MroXI	GAANNNNTTC	2 cut(s)	2589, 2836
MslI	CAYNNNNRTG	4 cut(s)	75, 893, 2529, 3140
MspA1I	CMGCKG	3 cut(s)	599, 1836, 2581
MspCI	CTTAAG	1 cut(s)	1535
MspI	CCGG	5 cut(s)	56, 413, 1171, 2410, 3041
MunI	CAATTG	2 cut(s)	1176, 1935
Mva1269I	GAATGC	3 cut(s)	1092, 3131, 3511
MvaI	CCWGG	8 cut(s)	423, 519, 1232, 1512, 2001, 3094, 3442, 3487
NciI	CCSGG	3 cut(s)	1172, 3041, 3042
NcoI	CCATGG	2 cut(s)	1962, 2798
NheI	GCTAGC	1 cut(s)	710
NmuCI	GTSAC	2 cut(s)	1693, 1807
NspI	RCATGY	3 cut(s)	80, 435, 1710
OliI	CACNNNNGTG	1 cut(s)	3140
PagI	TCATGA	2 cut(s)	1096, 2260
PaqCI	CACCTGC	1 cut(s)	1100
PciI	ACATGT	2 cut(s)	76, 431
PciSI	GCTCTTC	2 cut(s)	314, 3220
PcsI	WCGNNNNNNNCGW	1 cut(s)	1048
PctI	GAATGC	3 cut(s)	1092, 3131, 3511
PdmI	GAANNNNTTC	2 cut(s)	2589, 2836
PfeI	GAWTC	9 cut(s)	193, 546, 616, 757, 1382, 1529, 2900, 2926, 3132
PflMI	CCANNNNNTGG	1 cut(s)	3456
PfoI	TCCNGGA	1 cut(s)	1510
PinAI	ACCGGT	1 cut(s)	412
PleI	GAGTC	3 cut(s)	1386, 3118, 3149
PpsI	GAGTC	3 cut(s)	1386, 3118, 3149
PpuMI	RGGWCCY	4 cut(s)	2227, 3037, 3044, 3079
PscI	ACATGT	2 cut(s)	76, 431
Psp5II	RGGWCCY	4 cut(s)	2227, 3037, 3044, 3079
Psp6I	CCWGG	8 cut(s)	421, 517, 1230, 1510, 1999, 3092, 3440, 3485
PspGI	CCWGG	8 cut(s)	421, 517, 1230, 1510, 1999, 3092, 3440, 3485
PspOMI	GGGCCC	1 cut(s)	1637
PspPPI	RGGWCCY	4 cut(s)	2227, 3037, 3044, 3079
PstI	CTGCAG	2 cut(s)	696, 1414
PsuI	RGATCY	5 cut(s)	148, 652, 696, 1744, 2614
PvuII	CAGCTG	2 cut(s)	599, 2581
RsaI	GTAC	7 cut(s)	75, 158, 458, 1005, 1437, 2539, 3193
RsaNI	GTAC	7 cut(s)	74, 157, 457, 1004, 1436, 2538, 3192
RseI	CAYNNNNRTG	4 cut(s)	75, 893, 2529, 3140
SapI	GCTCTTC	2 cut(s)	314, 3220
ScaI	AGTACT	3 cut(s)	158, 1437, 2539
SchI	GAGTC	3 cut(s)	1386, 3119, 3149
SduI	GDGCHC	6 cut(s)	326, 635, 753, 1641, 2296, 2652
SfcI	CTRYAG	5 cut(s)	504, 594, 692, 1410, 1896
SinI	GGWCC	5 cut(s)	1048, 2227, 3037, 3044, 3079
SmaI	CCCGGG	1 cut(s)	3042
SmiMI	CAYNNNNRTG	4 cut(s)	75, 893, 2529, 3140
SmlI	CTYRAG	5 cut(s)	373, 1427, 1535, 2072, 3048
SmoI	CTYRAG	5 cut(s)	373, 1427, 1535, 2072, 3048
SpeI	ACTAGT	1 cut(s)	2570
SsiI	CCGC	9 cut(s)	524, 969, 983, 1155, 1267, 1836, 1944, 2119, 2974
SspI	AATATT	2 cut(s)	2455, 2470
StyI	CCWWGG	5 cut(s)	644, 1149, 1962, 2798, 3351
TaaI	ACNGT	7 cut(s)	205, 236, 1522, 1633, 1955, 2053, 3120
TaiI	ACGT	3 cut(s)	329, 1345, 3339
TaqI	TCGA	6 cut(s)	544, 956, 1416, 1920, 2924, 2929
TatI	WGTACW	6 cut(s)	73, 156, 1003, 1435, 2537, 3191
TauI	GCSGC	4 cut(s)	972, 1158, 1269, 2977
TfiI	GAWTC	9 cut(s)	193, 546, 616, 757, 1382, 1529, 2900, 2926, 3132
TscAI	CASTG	4 cut(s)	241, 257, 1527, 3145
TseFI	GTSAC	2 cut(s)	1693, 1807
Tsp45I	GTSAC	2 cut(s)	1693, 1807
TspDTI	ATGAA	8 cut(s)	24, 75, 195, 1085, 2703, 2829, 2862, 3088
TspGWI	ACGGA	4 cut(s)	645, 660, 1040, 2843
TspMI	CCCGGG	1 cut(s)	3040
TspRI	CASTG	4 cut(s)	241, 257, 1527, 3145
Van91I	CCANNNNNTGG	1 cut(s)	3456
Vha464I	CTTAAG	1 cut(s)	1535
VpaK11BI	GGWCC	5 cut(s)	1048, 2227, 3037, 3044, 3079
XapI	RAATTY	5 cut(s)	676, 740, 1466, 1647, 2849
XbaI	TCTAGA	1 cut(s)	2617
XceI	RCATGY	3 cut(s)	80, 435, 1710
XmaI	CCCGGG	1 cut(s)	3040
XmiI	GTMKAC	1 cut(s)	1593
XmnI	GAANNNNTTC	2 cut(s)	2589, 2836
ZrmI	AGTACT	3 cut(s)	158, 1437, 2539

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.