Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr1

Orthogroup ID

OG0005762

Physical Location & Seq

Genomic Coordinates Reverse (-)

49074843 .. 49079890

Gene Structure 5048 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000003485

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4383 bp

ATGTCCAGGAAGGACTATAATATCCTTGTCTTTTTGGGAAAATTACATGGGGATATATTTGTGGTTTTTGTCATAGTCTTCTTCTGTACTGAGACGCCACTCTCTTTTCTTGACCTCTCACGGCCACAGTGCAAGGTTGAGGAGATGGCTGGTGTTCGGGAGATCACTGCTGGTGAATTCATAAAAGGTGGCCATAAACAGGGCCTGTTTTCTTACAGTGGTGCTTTGAAAGGAAGAAGCCCTATGCTTTGGGGCACGCTGCACAGTTCAGTGCGGGGTTCTGCCAATAGGAGAAATGCTTCATTGAGATGTCGTGCTCAAGAGAAGCCCACAGCTGTTGTTTCGGCTGTCAGCAGTTCGGTGGAGGCGCTACCGACTGTGGTTGAGAAGCCTTCTTCCTCAGTTATCCATTTTTATCGTGTCCCTCTCATTCAGGAGAGTGCAACGTCTGAGCTTCTCAAAACCGTGCAGTCAAAGATTTCGGACCAGATAGTTGGTTTGAAAACTGAGCAGTGTTTCAACATTGGGCTCATTTCGCAGCTTTGTAGTGACAAGCTTTCGGTGCTTCAGTGGCTTCTTCAGGAGACTTATGAGCCGGAGAATTTGGGGGTCGACAGCTTTCTTGAAAAGAAAAGGCAGGAAGGACTGAACACAGTTATTGTTGAGGTTGGTCCTCGGTTGTCGTTTACAACAGCGTGGTCTTCCAATGCTGTCTCGATATGTAGAGCTTGTGGCTTGACAGAGGTGACTCGTTTGGAACAGTCAAGGAGATACTTGTTGTTTAGTAAGGGGAAGTTGCAGGATCAACAGATCAATGAGTTTGCTGCAATGGTTCATGATCGGATGACGGAGTGTGTTTACACCCAGCAGCTCACTTCTTTTGAAACAAGTGTGGTTCCTGACAAAGTACGCCATATTCCTGTCATGGAGAGGGGTCGGAAGGCGTTAGAGGAGATTAATCAGGAAATGGGTTTGGCATTTGATGAGCAAGATCTCCAATACTACACCAGGCTATTCAAGGAAGAGATCAAGCGCAATCCAACAACAGTTGAACTCTTCGATATTGCACAGTCCAACAGTGAGCATAGCAGGCACTGGTTTTTTACTGGTAAAATTTTTATAGATGGACAGCCCATGGACAAAACTCTAATGCAAATTGTGAAGAGCACTTTGCAAGCAAACCCAAACAATTCTGTCATCGGGTTTAAGGACAACTCGAGTGCAATCAGAGGATTTCTTGTGAAGCAATTGCGACCAGTTCAGCCAGGTTCAACATCTCCATTGCACATGTCTGCACGTGACCTTGATATATTATTTACTGCAGAGACCCATAATTTTCCATGTGCTGTAGCACCTTACCCTGGTGCAGAGACAGGTGCAGGAGGTAGAATCAGAGACACCCATGCAACTGGAAGGGGCTCTTTTGTGGTAGCATCTACTGCTGGTTATTGTGTCGGGAATCTCAATATGGAAGGATCTTATGCTCCTTGGGAAGATCCGTCTTTCTTGTATCCATCAAATTTGGCCTCACCTTTGCAGATCCTCATAGATGCTAGTAATGGTGCATCAGACTACGGCAACAAATTTGGTGAGCCCTTGATTCAGGGGTACACTAGAACCTTTGGAATGAGACTGCCAAGTGGTGAGAGGCGGGAATGGTTGAAGCCAATCATGTTCAGTGCAGGAATTGGACAGATTGATCACACCCATATAACAAAGGGAGAGCCTGATATTGGCATGCTAGTTGTAAAGATTGGAGGCCCTGCTTATCGTATTGGTATGGGTGGTGGGGCTGCATCGAGCATGGTTAGTGGCCAAAATGATGCGGAGCTTGATTTCAATGCTGTACAGCGTGGGGATGCTGAGATGGCACAAAAATTGTATCGTGTTGTTCGTGCCTGCATTGAGATGGGGGAGAGTAACCCAATCATTAGCATTCATGACCAAGGAGCTGGTGGAAACTGCAACGTGGTTAAGGAAATTATTTATCCGAAGGGTGGTGAGATTGATATCCGGGCAATTGTTGTTGGTGATTACACAATGTCTGTCTTGGAGATATGGGGCGCAGAATATCAGGAACAAGATGCCATCTTGGTTAAACCTGAAAGTCGCGAACTTTTGCAATCAATATGTGAAAGGGAAAGGTGTTCTATGGCTGTTATTGGAACAATAAATGGTGAGGGACGTATTGTTTTAATTGATAGTTTAGCTATTGAAAAATCTAAATCAAGTGGCCTCCCTCCACCTGATCCTGCTGTCGATTTGGAGCTTGAGAAAGTACTTGGTGATATGCCACAAAAAAGTTTTAAATTCGAGCGGATGGCAGATGCACGAGAGCCACTTGATATTGCCCCTGGAATCACAGTAATGGATGCTCTTAAGAGAGTTTTAAGACTTCCATCCATCTGTTCAAAGCGCTTCTTGACCTCAAAAGTAGACAGGTGTGTTACAGGTCTGGTAGCTCAGCAGCAAACTGTCGGGCCCCTGCAAATTCCTCTCTCTGATGTTGGAGTAATTGCTCAGACTTTTACTGGCTTGACTGGAGGTGCATGTGCCATTGGGGAGCAGCCAATCAAAGGTTTGTTGGATCCAAAAGCAATGGCTAGATTGGCAGTTGGAGAAGCACTCACAAATCTTGTTTGGGCAAAGGTCACTTCTCTATCTGATGTTAAAGCAAGTGGGAATTGGATGTATGCAGCCAAGCTTGATGGTGAAGGAGCAGCCATGTATGATGCTGCTAATGCTCTTTCAGAGGCGATGATCAAACTTGGTATTGCTATTGATGGGGGAAAGGACAGTCTTTCCATGGCAGCCCATGCTGCAGGAGAGGTTGTTAAGGCTCCTGGAAATCTTGTAATCAGTGTTTATTGTACTTGTCCTGACATAACAAAAACAGTAACCCCAGATTTGAAGCTTGGAGATGATGGTGTTCTGCTTCACATTGATTTGGCCGCAGGAAAGCGGCGATTAGGTGGCTCTGCTCTTGCTCAGGTTTTTGACCAGATTGGGAACGATTGTCCTGACCTTGAGGATGTTCCGTATCTGAAGCGAGTGTTTGAGGGAGTTCAAGGCCTTCTTGATGATGAACTGATCTCTGCTGGCCATGATATCAGTGATGGTGGGCTTCTGGTCTGTGCCCTTGAGATGGCATTTGCTGGCAATTGTGGCATTAATTTGGACTTGACTTCACACCGGAAAAGCCTCTTCCAAACAATTTTTTCTGAAGAGCTTGGTCTGATTATTGAGGTCAGCAAGAAAAACTTAGATATGGTAATAGGAAAGCTAAGCAGTGGAGGTATTTCTGCAGAGATTATTGGACAGGTAACTGCCACACCTTCAATAGTGTTAAAGGTGGATGGGGTAACTCATTTGAACGAAAGTACTTCTTTTGTAAGGGACATGTGGGAGGATACTAGTTTTCAGCTGGAAAATTTTCAGAGATTGGCTTCTTGTGTTGATCTAGAGAAAGAAGGGTTGAAAGATAGGCATGAGCCTTCATGGCAGTTGTCTTTTACTCCATCCTTCACAGATGAAAAGTATATGACCACGACCTCCAAACCGAAGGTGGCTGTTATTCGAGAGGAAGGAAGCAATGGGGATAGAGAAATGGCTGCGGCATTTTATGCTGCTGGCTTTGAGCCATGGGATATTGCAATGTCAGACCTTCTTAATGGAGCTGTATCACTGCAAGAGTTCCGTGGAATTGTGTTTGTTGGAGGTTTTAGCTATGCTGATGTCCTTGATTCTGCAAAAGGGTGGTCTGCTTCAATTAGATTTAATCAGCCCCTTCTAAATCAATTTCAGGAGTTTTACAAGAGGCACGACACTTTCAGTCTTGGCGTTTGCAATGGGTGTCAGCTTATGGCTCTTTTGGGCTGGGTCCCAGGTCCTCAAGTTGGGGGTGTGCATGGTGGTGGTGGGGATCCATCACAGCCGAGGTTCATTCACAATGAGTCGGGCCGGTTTGAGTGTCGCTTTACTAGTGTAAAAATTAAGGACTCACCTTCTATAATGTTAAAAGGAATGGAAGGCAGTACTTTGGGCGTGTGGGCTGCCCATGGTGAGGGAAGAGCCTATTTTCCTGATGATGGTGTTTTTGATCGTGTGCTCCACTCCAAGTTGGCTCCAGTAAGATATTGTGATGATGATGGGATTGAAACGGAACTTTATCCCTTCAACCTGAATGGCTCTCCCTTGGGAGTTGCGGCAATTTGTTCACCAGATGGGAGGCATCTTGCCATGATGCCTCATCCGGAGCGCTGCTTCTTAATGTGGCAGTACCCATGGTATCCCAAGCAGTGGGATGTTGAAAAGAAAGGCCCTAGTCCATGGCTACGGATGTTCCAGAATGCCAGAGAGTGGTGCTCCAGAAATGATGCCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0003674 GO:0003824 GO:0004642 GO:0005488 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0007275 GO:0008144 GO:0008150 GO:0009507 GO:0009532 GO:0009536 GO:0009555 GO:0009570 GO:0016874 GO:0016879 GO:0016884 GO:0017076 GO:0030554 GO:0032501 GO:0032502 GO:0032553 GO:0032555 GO:0032559 GO:0035639 GO:0036094 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0048229 GO:0048856 GO:0055046 GO:0097159 GO:0097367 GO:1901265 GO:1901363

KEGG Pathways

Metabolic & Signaling

ko00230 ko01100 ko01110 ko01130 map00230 map01100 map01110 map01130

Pfam Domains

Protein Families

AIRS_C GATase_5

Protein Analysis

1461

Amino Acids

159.79

Weight (kDa)

5.46

Isoelectric Point (pI)

38.09

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FGAR-AT_N	PF18076	173 - 290	3.5e-20	Formylglycinamide ribonucleotide amidotransferase N-terminal
FGAR-AT_linker	PF18072	316 - 365	5e-11	Formylglycinamide ribonucleotide amidotransferase linker domain
AIRS_C	PF02769	579 - 733	2e-27	AIR synthase related protein, C-terminal domain
FGAR-AT_PurM_N-like	PF22689	804 - 957	2.2e-52	FGAR-AT PurM_N-like domain
AIRS_C	PF02769	988 - 1118	7.4e-19	AIR synthase related protein, C-terminal domain
GATase_5	PF13507	1186 - 1455	6.5e-105	CobB/CobQ-like glutamine amidotransferase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0005762)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G74260
fragaria_vesca	FvH4_5g19530 FvH4_5g19530 FvH4_5g19530
malus_domestica	MD03G1203100.v1.1 MD04G1022300.v1.1
prunus_persica	Prupe.1G542100_v2.0.a1 Prupe.1G542100_v2.0.a1 Prupe.1G542100_v2.0.a1 Prupe.1G542100_v2.0.a1 Prupe.1G542100_v2.0.a1 Prupe.8G024000_v2.0.a1 Prupe.8G024000_v2.0.a1 Prupe.8G024000_v2.0.a1
pyrus_communis	pycom04g01890 pycom04g01930
rosa_chinensis	RchiOBHm_Chr7g0204141
rosa_laevigata	RLG00000003485
rosa_multiflora	Rmu_sc0001311.1_g000031
rosa_roxburghii	Rroxscaffold_3G00253310
rosa_rugosa	Rorug07G0081100 Rorug07G0081200 Rorug07G0081200
rosa_samantha	Rh7AG211000 Rh7BG209600 Rh7CG223500 Rh7DG217800
rosa_wichuraiana	Rw7G018360

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	2 cut(s)	4299, 4359
AccBSI	CCGCTC	1 cut(s)	2317
AccI	GTMKAC	2 cut(s)	612, 2436
AccII	CGCG	1 cut(s)	2112
AccIII	TCCGGA	1 cut(s)	4252
AciI	CCGC	8 cut(s)	274, 1651, 1826, 2317, 2952, 2962, 3614, 4205
AclWI	GGATC	9 cut(s)	810, 1483, 1490, 1534, 2243, 2582, 2595, 3917, 3930
AcoI	YGGCCR	5 cut(s)	122, 190, 1813, 2949, 3100
AcsI	RAATTY	8 cut(s)	176, 601, 1113, 1519, 1583, 2309, 2490, 3430
AcuI	CTGAAG	4 cut(s)	551, 563, 3065, 3243
AcvI	CACGTG	1 cut(s)	1298
AcyI	GRCGYC	1 cut(s)	95
AfaI	GTAC	9 cut(s)	88, 909, 1610, 1848, 2280, 2872, 3382, 4036, 4280
AfeI	AGCGCT	2 cut(s)	2417, 4259
AflII	CTTAAG	1 cut(s)	2378
AflIII	ACRYGT	2 cut(s)	1287, 3399
AhlI	ACTAGT	2 cut(s)	3413, 3980
AjnI	CCWGG	7 cut(s)	5, 1007, 1264, 1360, 2353, 2842, 3883
Alw21I	GWGCWC	4 cut(s)	319, 1169, 4110, 4367
Alw26I	GTCTC	7 cut(s)	86, 578, 718, 1319, 1364, 1389, 1624
AlwI	GGATC	9 cut(s)	810, 1483, 1490, 1534, 2243, 2582, 2595, 3917, 3930
AlwNI	CAGNNNCTG	2 cut(s)	205, 1095
Ama87I	CYCGRG	1 cut(s)	1216
Aor13HI	TCCGGA	1 cut(s)	4252
Aor51HI	AGCGCT	2 cut(s)	2417, 4259
ApaI	GGGCCC	1 cut(s)	2484
ApoI	RAATTY	8 cut(s)	176, 601, 1113, 1519, 1583, 2309, 2490, 3430
ArsI	GACNNNNNNTTYG	2 cut(s)	3743, 3775
AseI	ATTAAT	2 cut(s)	957, 3171
Asp700I	GAANNNNTTC	2 cut(s)	298, 3432
AspLEI	GCGC	5 cut(s)	370, 1035, 2066, 2418, 4260
AsuC2I	CCSGG	1 cut(s)	2015
AvaI	CYCGRG	1 cut(s)	1216
AvaII	GGWCC	4 cut(s)	484, 671, 3880, 3887
BaeGI	GKGCMC	3 cut(s)	257, 2484, 3139
BalI	TGGCCA	3 cut(s)	192, 1815, 3102
BamHI	GGATCC	2 cut(s)	2587, 3922
BanII	GRGCYC	4 cut(s)	531, 1421, 1596, 2484
BauI	CACGAG	1 cut(s)	2331
BbrPI	CACGTG	1 cut(s)	1298
BbsI	GAAGAC	2 cut(s)	70, 693
Bbv12I	GWGCWC	4 cut(s)	319, 1169, 4110, 4367
BceAI	ACGGC	2 cut(s)	137, 1591
BciT130I	CCWGG	7 cut(s)	7, 1009, 1266, 1362, 2355, 2844, 3885
BciVI	GTATCC	3 cut(s)	1521, 3403, 4299
BclI	TGATCA	2 cut(s)	1699, 2760
BcnI	CCSGG	1 cut(s)	2015
BcoDI	GTCTC	7 cut(s)	86, 578, 718, 1319, 1364, 1389, 1624
BcuI	ACTAGT	2 cut(s)	3413, 3980
BfaI	CTAG	8 cut(s)	1554, 1614, 1742, 2604, 3414, 3461, 3981, 4323
BfmI	CTRYAG	4 cut(s)	1320, 1347, 2820, 3303
BfoI	RGCGCY	3 cut(s)	371, 2419, 4261
BfrI	CTTAAG	1 cut(s)	2378
BfuI	GTATCC	3 cut(s)	1521, 3403, 4299
BglI	GCCNNNNNGGC	1 cut(s)	3499
BglII	AGATCT	1 cut(s)	991
BlpI	GCTNAGC	2 cut(s)	2463, 3284
BmcAI	AGTACT	3 cut(s)	2280, 3382, 4036
Bme1390I	CCNGG	8 cut(s)	7, 1009, 1266, 1362, 2015, 2355, 2844, 3885
Bme18I	GGWCC	4 cut(s)	484, 671, 3880, 3887
BmeT110I	CYCGRG	1 cut(s)	1216
BmiI	GGNNCC	9 cut(s)	897, 2482, 2483, 2589, 2841, 3881, 3882, 3924, 4125
BmrFI	CCNGG	8 cut(s)	7, 1009, 1266, 1362, 2015, 2355, 2844, 3885
BpiI	GAAGAC	2 cut(s)	70, 693
BplI	GAGNNNNNCTC	4 cut(s)	2610, 2642, 4349, 4381
BpmI	CTGGAG	3 cut(s)	2562, 4110, 4351
Bpu10I	CCTNAGC	1 cut(s)	2988
Bpu1102I	GCTNAGC	2 cut(s)	2463, 3284
BpuEI	CTTGAG	5 cut(s)	303, 2291, 3047, 3161, 3876
BpuMI	CCSGG	1 cut(s)	2015
BsaAI	YACGTR	1 cut(s)	1298
BsaBI	GATNNNNATC	1 cut(s)	2009
BsaHI	GRCGYC	1 cut(s)	95
BsaI	GGTCTC	1 cut(s)	1319
BsaWI	WCCGGW	2 cut(s)	3192, 4252
BsaXI	ACNNNNNCTCC	2 cut(s)	2817, 2847
Bse118I	RCCGGY	1 cut(s)	3960
Bse1I	ACTGG	7 cut(s)	1100, 1111, 1256, 1414, 2536, 2545, 4127
Bse3DI	GCAATG	6 cut(s)	834, 1280, 2604, 3598, 3660, 3853
Bse8I	GATNNNNATC	1 cut(s)	2009
BseAI	TCCGGA	1 cut(s)	4252
BseBI	CCWGG	7 cut(s)	7, 1009, 1266, 1362, 2355, 2844, 3885
BseJI	GATNNNNATC	1 cut(s)	2009
BseMI	GCAATG	6 cut(s)	834, 1280, 2604, 3598, 3660, 3853
BseMII	CTCAG	8 cut(s)	81, 414, 441, 498, 1854, 2477, 2534, 3002
BseNI	ACTGG	7 cut(s)	1100, 1111, 1256, 1414, 2536, 2545, 4127
BseRI	GAGGAG	2 cut(s)	155, 965
BseSI	GKGCMC	3 cut(s)	257, 2484, 3139
BseYI	CCCAGC	2 cut(s)	864, 3876
BsgI	GTGCAG	6 cut(s)	245, 488, 1278, 1386, 1398, 1701
Bsh1236I	CGCG	1 cut(s)	2112
BsiHKAI	GWGCWC	4 cut(s)	319, 1169, 4110, 4367
BsiHKCI	CYCGRG	1 cut(s)	1216
BsiSI	CCGG	5 cut(s)	596, 2014, 3193, 3961, 4253
BslFI	GGGAC	4 cut(s)	407, 2196, 3410, 3866
BsmAI	GTCTC	7 cut(s)	86, 578, 718, 1319, 1364, 1389, 1624
BsmBI	CGTCTC	1 cut(s)	86
BsmFI	GGGAC	4 cut(s)	407, 2196, 3410, 3866
BsmI	GAATGC	2 cut(s)	1935, 4354
Bso31I	GGTCTC	1 cut(s)	1319
BsoBI	CYCGRG	1 cut(s)	1216
Bsp120I	GGGCCC	1 cut(s)	2480
Bsp13I	TCCGGA	1 cut(s)	4252
Bsp1407I	TGTACA	1 cut(s)	1846
Bsp1720I	GCTNAGC	2 cut(s)	2463, 3284
Bsp19I	CCATGG	6 cut(s)	1134, 2805, 3641, 4057, 4283, 4328
Bsp68I	TCGCGA	1 cut(s)	2112
BspACI	CCGC	8 cut(s)	274, 1651, 1826, 2317, 2952, 2962, 3614, 4205
BspCNI	CTCAG	8 cut(s)	82, 413, 442, 499, 1855, 2476, 2533, 3001
BspEI	TCCGGA	1 cut(s)	4252
BspFNI	CGCG	1 cut(s)	2112
BspHI	TCATGA	2 cut(s)	835, 1939
BspLI	GGNNCC	9 cut(s)	897, 2482, 2483, 2589, 2841, 3881, 3882, 3924, 4125
BspMAI	CTGCAG	3 cut(s)	1324, 2824, 3307
BspPI	GGATC	9 cut(s)	810, 1483, 1490, 1534, 2243, 2582, 2595, 3917, 3930
BspQI	GCTCTTC	3 cut(s)	1157, 3219, 4063
BspTI	CTTAAG	1 cut(s)	2378
BspTNI	GGTCTC	1 cut(s)	1319
BsrBI	CCGCTC	1 cut(s)	2317
BsrDI	GCAATG	6 cut(s)	834, 1280, 2604, 3598, 3660, 3853
BsrFI	RCCGGY	1 cut(s)	3960
BsrGI	TGTACA	1 cut(s)	1846
BsrI	ACTGG	7 cut(s)	1100, 1111, 1256, 1414, 2536, 2545, 4127
BssAI	RCCGGY	1 cut(s)	3960
BssNI	GRCGYC	1 cut(s)	95
BssSI	CACGAG	1 cut(s)	2331
BssT1I	CCWWGG	9 cut(s)	1134, 1487, 1945, 2805, 3641, 4057, 4194, 4283, 4328
Bst2BI	CACGAG	1 cut(s)	2331
Bst2UI	CCWGG	7 cut(s)	7, 1009, 1266, 1362, 2355, 2844, 3885
Bst6I	CTCTTC	6 cut(s)	1017, 1061, 1157, 3209, 3219, 4063
BstACI	GRCGYC	1 cut(s)	95
BstAFI	CTTAAG	1 cut(s)	2378
BstAPI	GCANNNNNTGC	3 cut(s)	1439, 2816, 3623
BstAUI	TGTACA	1 cut(s)	1846
BstBAI	YACGTR	1 cut(s)	1298
BstC8I	GCNNGC	8 cut(s)	257, 1091, 1176, 1739, 1900, 3100, 3157, 3631
BstDSI	CCRYGG	7 cut(s)	1134, 2805, 3641, 3697, 4057, 4283, 4328
BstFNI	CGCG	1 cut(s)	2112
BstH2I	RGCGCY	3 cut(s)	371, 2419, 4261
BstHHI	GCGC	5 cut(s)	370, 1035, 2066, 2418, 4260
BstMAI	GTCTC	7 cut(s)	86, 578, 718, 1319, 1364, 1389, 1624
BstNI	CCWGG	7 cut(s)	7, 1009, 1266, 1362, 2355, 2844, 3885
BstNSI	RCATGY	4 cut(s)	1291, 1741, 2553, 3403
BstSCI	CCNGG	8 cut(s)	5, 1007, 1264, 1360, 2013, 2353, 2842, 3883
BstSFI	CTRYAG	4 cut(s)	1320, 1347, 2820, 3303
BstSLI	GKGCMC	3 cut(s)	257, 2484, 3139
BstUI	CGCG	1 cut(s)	2112
BstV2I	GAAGAC	2 cut(s)	70, 693
BstX2I	RGATCY	6 cut(s)	991, 1475, 1495, 1539, 2587, 3922
BstXI	CCANNNNNNTGG	3 cut(s)	494, 1409, 1952
BstYI	RGATCY	6 cut(s)	991, 1475, 1495, 1539, 2587, 3922
BsuI	GTATCC	3 cut(s)	1521, 3403, 4299
BtgI	CCRYGG	7 cut(s)	1134, 2805, 3641, 3697, 4057, 4283, 4328
BtgZI	GCGATG	1 cut(s)	2771
BtsI	GCAGTG	5 cut(s)	165, 518, 3295, 3683, 4304
BtuMI	TCGCGA	1 cut(s)	2112
Cac8I	GCNNGC	8 cut(s)	257, 1091, 1176, 1739, 1900, 3100, 3157, 3631
CaiI	CAGNNNCTG	2 cut(s)	205, 1095
CciI	TCATGA	2 cut(s)	835, 1939
CfoI	GCGC	5 cut(s)	370, 1035, 2066, 2418, 4260
Cfr10I	RCCGGY	1 cut(s)	3960
CseI	GACGC	1 cut(s)	103
Csp6I	GTAC	9 cut(s)	87, 908, 1609, 1847, 2279, 2871, 3381, 4035, 4279
CspCI	CAANNNNNGTGG	2 cut(s)	319, 354
CviQI	GTAC	9 cut(s)	87, 908, 1609, 1847, 2279, 2871, 3381, 4035, 4279
DraI	TTTAAA	1 cut(s)	2308
EaeI	YGGCCR	5 cut(s)	122, 190, 1813, 2949, 3100
Eam1104I	CTCTTC	6 cut(s)	1017, 1061, 1157, 3209, 3219, 4063
EarI	CTCTTC	6 cut(s)	1017, 1061, 1157, 3209, 3219, 4063
Eco130I	CCWWGG	9 cut(s)	1134, 1487, 1945, 2805, 3641, 4057, 4194, 4283, 4328
Eco147I	AGGCCT	1 cut(s)	3072
Eco24I	GRGCYC	4 cut(s)	531, 1421, 1596, 2484
Eco31I	GGTCTC	1 cut(s)	1319
Eco32I	GATATC	2 cut(s)	2011, 3109
Eco47I	GGWCC	4 cut(s)	484, 671, 3880, 3887
Eco47III	AGCGCT	2 cut(s)	2417, 4259
Eco57I	CTGAAG	4 cut(s)	551, 563, 3065, 3243
Eco72I	CACGTG	1 cut(s)	1298
Eco88I	CYCGRG	1 cut(s)	1216
EcoO109I	RGGNCCY	6 cut(s)	202, 1760, 2481, 3880, 3887, 4319
EcoRI	GAATTC	1 cut(s)	176
EcoRII	CCWGG	7 cut(s)	5, 1007, 1264, 1360, 2353, 2842, 3883
EcoRV	GATATC	2 cut(s)	2011, 3109
EcoT14I	CCWWGG	9 cut(s)	1134, 1487, 1945, 2805, 3641, 4057, 4194, 4283, 4328
EcoT38I	GRGCYC	4 cut(s)	531, 1421, 1596, 2484
ErhI	CCWWGG	9 cut(s)	1134, 1487, 1945, 2805, 3641, 4057, 4194, 4283, 4328
Esp3I	CGTCTC	1 cut(s)	86
FaqI	GGGAC	4 cut(s)	407, 2196, 3410, 3866
FauI	CCCGC	2 cut(s)	267, 1644
FbaI	TGATCA	2 cut(s)	1699, 2760
FblI	GTMKAC	2 cut(s)	612, 2436
FriOI	GRGCYC	4 cut(s)	531, 1421, 1596, 2484
FspBI	CTAG	8 cut(s)	1554, 1614, 1742, 2604, 3414, 3461, 3981, 4323
GlaI	GCGC	5 cut(s)	369, 1034, 2065, 2417, 4259
GsaI	CCCAGC	2 cut(s)	868, 3880
GsuI	CTGGAG	3 cut(s)	2562, 4110, 4351
HaeII	RGCGCY	3 cut(s)	371, 2419, 4261
HapII	CCGG	5 cut(s)	596, 2014, 3193, 3961, 4253
HgaI	GACGC	1 cut(s)	103
HhaI	GCGC	5 cut(s)	370, 1035, 2066, 2418, 4260
Hin1I	GRCGYC	1 cut(s)	95
Hin6I	GCGC	5 cut(s)	368, 1033, 2064, 2416, 4258
HinP1I	GCGC	5 cut(s)	368, 1033, 2064, 2416, 4258
HincII	GTYRAC	1 cut(s)	613
HindII	GTYRAC	1 cut(s)	613
HindIII	AAGCTT	3 cut(s)	554, 2702, 2912
HinfI	GANTC	8 cut(s)	748, 1389, 1459, 1600, 2358, 3743, 3953, 3998
HpaII	CCGG	5 cut(s)	596, 2014, 3193, 3961, 4253
Hpy166II	GTNNAC	6 cut(s)	613, 687, 859, 1611, 2437, 4217
Hpy8I	GTNNAC	6 cut(s)	613, 687, 859, 1611, 2437, 4217
HpyCH4IV	ACGT	4 cut(s)	446, 1297, 1968, 2185
HpySE526I	ACGT	4 cut(s)	446, 1297, 1968, 2185
Hsp92I	GRCGYC	1 cut(s)	95
HspAI	GCGC	5 cut(s)	368, 1033, 2064, 2416, 4258
KflI	GGGWCCC	1 cut(s)	3880
Kpn2I	TCCGGA	1 cut(s)	4252
Ksp22I	TGATCA	2 cut(s)	1699, 2760
LguI	GCTCTTC	3 cut(s)	1157, 3219, 4063
MaeI	CTAG	8 cut(s)	1554, 1614, 1742, 2604, 3414, 3461, 3981, 4323
MaeII	ACGT	4 cut(s)	446, 1297, 1968, 2185
MaeIII	GTNAC	9 cut(s)	548, 745, 1298, 1919, 2446, 2650, 2896, 3322, 3361
MbiI	CCGCTC	1 cut(s)	2317
MfeI	CAATTG	3 cut(s)	1247, 2019, 3160
MflI	RGATCY	6 cut(s)	991, 1475, 1495, 1539, 2587, 3922
MlsI	TGGCCA	3 cut(s)	192, 1815, 3102
MluNI	TGGCCA	3 cut(s)	192, 1815, 3102
MlyI	GAGTC	3 cut(s)	742, 3962, 3992
MmeI	TCCRAC	7 cut(s)	917, 1064, 1098, 2488, 2565, 2596, 3694
Mox20I	TGGCCA	3 cut(s)	192, 1815, 3102
MroI	TCCGGA	1 cut(s)	4252
MroXI	GAANNNNTTC	2 cut(s)	298, 3432
MscI	TGGCCA	3 cut(s)	192, 1815, 3102
MslI	CAYNNNNRTG	4 cut(s)	307, 1907, 2366, 3912
Msp20I	TGGCCA	3 cut(s)	192, 1815, 3102
MspA1I	CMGCKG	2 cut(s)	335, 3424
MspCI	CTTAAG	1 cut(s)	2378
MspI	CCGG	5 cut(s)	596, 2014, 3193, 3961, 4253
MspR9I	CCNGG	8 cut(s)	7, 1009, 1266, 1362, 2015, 2355, 2844, 3885
MunI	CAATTG	3 cut(s)	1247, 2019, 3160
Mva1269I	GAATGC	2 cut(s)	1935, 4354
MvaI	CCWGG	7 cut(s)	7, 1009, 1266, 1362, 2355, 2844, 3885
MvnI	CGCG	1 cut(s)	2112
NciI	CCSGG	1 cut(s)	2015
NcoI	CCATGG	6 cut(s)	1134, 2805, 3641, 4057, 4283, 4328
NlaIV	GGNNCC	9 cut(s)	897, 2482, 2483, 2589, 2841, 3881, 3882, 3924, 4125
NmeAIII	GCCGAG	1 cut(s)	3960
NmuCI	GTSAC	4 cut(s)	548, 745, 1298, 2650
NruI	TCGCGA	1 cut(s)	2112
NspI	RCATGY	4 cut(s)	1291, 1741, 2553, 3403
PaeI	GCATGC	1 cut(s)	1741
PaeR7I	CTCGAG	1 cut(s)	1216
PagI	TCATGA	2 cut(s)	835, 1939
PceI	AGGCCT	1 cut(s)	3072
PciI	ACATGT	2 cut(s)	1287, 3399
PciSI	GCTCTTC	3 cut(s)	1157, 3219, 4063
PcsI	WCGNNNNNNNCGW	1 cut(s)	1891
PctI	GAATGC	2 cut(s)	1935, 4354
PdmI	GAANNNNTTC	2 cut(s)	298, 3432
PfeI	GAWTC	5 cut(s)	1389, 1459, 1600, 2358, 3743
PflMI	CCANNNNNTGG	2 cut(s)	4299, 4359
PfoI	TCCNGGA	2 cut(s)	5, 2842
PleI	GAGTC	3 cut(s)	742, 3961, 3992
PmaCI	CACGTG	1 cut(s)	1298
PmlI	CACGTG	1 cut(s)	1298
PpsI	GAGTC	3 cut(s)	742, 3961, 3992
Ppu21I	YACGTR	1 cut(s)	1298
PpuMI	RGGWCCY	2 cut(s)	3880, 3887
PscI	ACATGT	2 cut(s)	1287, 3399
PshBI	ATTAAT	2 cut(s)	957, 3171
Psp5II	RGGWCCY	2 cut(s)	3880, 3887
Psp6I	CCWGG	7 cut(s)	5, 1007, 1264, 1360, 2353, 2842, 3883
PspCI	CACGTG	1 cut(s)	1298
PspFI	CCCAGC	2 cut(s)	864, 3876
PspGI	CCWGG	7 cut(s)	5, 1007, 1264, 1360, 2353, 2842, 3883
PspN4I	GGNNCC	9 cut(s)	897, 2482, 2483, 2589, 2841, 3881, 3882, 3924, 4125
PspOMI	GGGCCC	1 cut(s)	2480
PspPPI	RGGWCCY	2 cut(s)	3880, 3887
PspXI	VCTCGAGB	1 cut(s)	1216
PstI	CTGCAG	3 cut(s)	1324, 2824, 3307
PstNI	CAGNNNCTG	2 cut(s)	205, 1095
PsuI	RGATCY	6 cut(s)	991, 1475, 1495, 1539, 2587, 3922
PvuII	CAGCTG	2 cut(s)	335, 3424
RruI	TCGCGA	1 cut(s)	2112
RsaI	GTAC	9 cut(s)	88, 909, 1610, 1848, 2280, 2872, 3382, 4036, 4280
RsaNI	GTAC	9 cut(s)	87, 908, 1609, 1847, 2279, 2871, 3381, 4035, 4279
RseI	CAYNNNNRTG	4 cut(s)	307, 1907, 2366, 3912
SalI	GTCGAC	1 cut(s)	611
SapI	GCTCTTC	3 cut(s)	1157, 3219, 4063
ScaI	AGTACT	3 cut(s)	2280, 3382, 4036
SchI	GAGTC	3 cut(s)	742, 3962, 3992
ScrFI	CCNGG	8 cut(s)	7, 1009, 1266, 1362, 2015, 2355, 2844, 3885
SfcI	CTRYAG	4 cut(s)	1320, 1347, 2820, 3303
Sfr274I	CTCGAG	1 cut(s)	1216
SinI	GGWCC	4 cut(s)	484, 671, 3880, 3887
SlaI	CTCGAG	1 cut(s)	1216
SmiMI	CAYNNNNRTG	4 cut(s)	307, 1907, 2366, 3912
SmlI	CTYRAG	7 cut(s)	318, 1216, 2270, 2378, 3026, 3140, 3891
SmoI	CTYRAG	7 cut(s)	318, 1216, 2270, 2378, 3026, 3140, 3891
SpeI	ACTAGT	2 cut(s)	3413, 3980
SphI	GCATGC	1 cut(s)	1741
SseBI	AGGCCT	1 cut(s)	3072
SsiI	CCGC	8 cut(s)	274, 1651, 1826, 2317, 2952, 2962, 3614, 4205
SspMI	CTAG	8 cut(s)	1554, 1614, 1742, 2604, 3414, 3461, 3981, 4323
StuI	AGGCCT	1 cut(s)	3072
StyD4I	CCNGG	8 cut(s)	5, 1007, 1264, 1360, 2013, 2353, 2842, 3883
StyI	CCWWGG	9 cut(s)	1134, 1487, 1945, 2805, 3641, 4057, 4194, 4283, 4328
TaiI	ACGT	4 cut(s)	449, 1300, 1971, 2188
TaqI	TCGA	8 cut(s)	612, 716, 1059, 1217, 1799, 2259, 2313, 3577
TatI	WGTACW	6 cut(s)	86, 1846, 2278, 2870, 3380, 4034
TauI	GCSGC	4 cut(s)	2954, 2965, 3617, 4208
TfiI	GAWTC	5 cut(s)	1389, 1459, 1600, 2358, 3743
TseFI	GTSAC	4 cut(s)	548, 745, 1298, 2650
Tsp45I	GTSAC	4 cut(s)	548, 745, 1298, 2650
TspDTI	ATGAA	8 cut(s)	169, 291, 824, 1928, 3099, 3486, 3546, 3931
TspGWI	ACGGA	6 cut(s)	863, 1488, 3027, 3686, 4175, 4351
Van91I	CCANNNNNTGG	2 cut(s)	4299, 4359
Vha464I	CTTAAG	1 cut(s)	2378
VpaK11BI	GGWCC	4 cut(s)	484, 671, 3880, 3887
VspI	ATTAAT	2 cut(s)	957, 3171
XapI	RAATTY	8 cut(s)	176, 601, 1113, 1519, 1583, 2309, 2490, 3430
XbaI	TCTAGA	1 cut(s)	3460
XceI	RCATGY	4 cut(s)	1291, 1741, 2553, 3403
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1952, 3562
XhoI	CTCGAG	1 cut(s)	1216
XmiI	GTMKAC	2 cut(s)	612, 2436
XmnI	GAANNNNTTC	2 cut(s)	298, 3432
XspI	CTAG	8 cut(s)	1554, 1614, 1742, 2604, 3414, 3461, 3981, 4323
ZrmI	AGTACT	3 cut(s)	2280, 3382, 4036

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.