Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0005762

Physical Location & Seq

Genomic Coordinates Forward (+)

21711690 .. 21717251

Gene Structure 5562 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ18270

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4239 bp

ATGGCTGGTGTTCGGGAGATCACTGCTGGTGAATTCATAAAAGGTGGCCATAAACAGGGCCTGTTTTCTCACAGAGGTGCTTTGAAAGGAAGAAGCCCTATGCTTTGGGGCACGCTGCACAGTTCAGTGCGGGGTTCTGCCAATAGGAGAAATGCTTCATTGAGATGTCGTGCTCAAGAGAAGCCCACAGCTGTTGTTTCGGCTGTCAGCAGTTCGGTGGAGGCGCTACCGACTGTGGTTGAGAAGCCTTCTTCCTCAGTTATCCATTTTTATCGTGTCCCTCTCATTCAGGAGAGTGCAGCGTCTGAGCTTCTCAAAACAGTGCAGTCAAAGATTTCGAGCCAGATAGTTGGTTTGAAAACTGAGCAGTGTTTCAACATTGGGCTCATTTCACAGCTTTCTAGTGACAAGCTTTTAGTGCTTAACTGGCTTCTTCAGGAGACTTATGAGCCAGAGAATTTGGGGGTCGACAGCTTTCTTGAAAAGAAGAGGCAGGAAGGGCTGAACACAGTTATTGTTGAGGTTGGTCCTCGGTTGTCGTTTACAACAGCGTGGTCTTCCAATGCTGTCTCGATATGTAGAGCTTGTGGCTTGACAGAGGTGACTCGTTTGGAACGGTCGAGGAGGTACTTGTTGTTTAGTAAGGGAAACTTGCAGGATCAACAGATTAATGAGTTTGCTGCAATGGTTCATGATCGGATGACGGAGTGTGTTTACACCCAGCAGCTCACTTCTTTTGAAACAAGTTTGGTTCCCGACAAAGTACGCCATATTCCTGTCATGGAGAGAGGTCGGAAGGCGTTAGAGGAGATTAATCAGGAAATGGGTTTGGCATTTGATGAGCAAGATCTCCAATACTACACCAGGCTATTCAAGGAAGAGATCAAGCGGAATCCAACAACAGTTGAACTCTTTGATATTGCACAGTCCAACAGTGAGCATAGCAGGCACTGGTTTTTTACTGGTAAAATTTTTATAGATGGACAGCCCATGGACAAAACTCTAATGCAAATTGTGAAGAGCACTTTGCAAGCAAACCCAAACAATTCTGTCATTGGGTTTAAGGACAACTCGAGTGCAATCAGAGGATTTCTTGTGAAGCAATTGCGACCGGTTCAGCCAGGTTCAACATCTCCATTGCACATGTCTGCACGTGACCTTGATATACTATTTACTGCAGAGACCCATAATTTTCCATGTGCTGTAGCACCTTACCCTGGTGCAGAGACAGGTGCAGGAGGTAGAATCAGAGACACCCATGCAACTGGAAGGGGCTCTTTTGTGGTAGCATCTACTGCTGGTTATTGTGTCGGAAATCTCAATATGGAAGGATCTTATGCTCCTTGGGAAGATCCGTCTTTCCTGTATCCATCAAATTTGGCCTCACCTTTGCAGATCCTCATAGATGCTAGTAATGGTGCATCAGACTACGGCAACAAATTTGGTGAGCCCTTGATTCAGGGTTACACTAGAACCTTTGGAATGAGACTCCCAAGTGGTGAGAGGCGGGAATGGTTGAAGCCAATCATGTTCAGTGCAGGAATTGGACAGATTGATCACACCCATATAACAAAGGGAGAGCCTGATATTGGCATGCTAGTTGTAAAGATTGGAGGCCCTGCTTATCGTATTGGTATGGGTGGTGGGGCTGCATCGAGCATGGTTAGTGGCCAAAATGATGCGGAGCTTGATTTCAATGCTGTACAGCGTGGGGATGCAGAGATGGCACAAAAATTGTATCGTGTTGTTCGTGCCTGCATTGAGATGGGGGAGAGTAACCCAATCATTAGCATTCATGACCAAGGAGCTGGTGGAAACTGCAACGTGGTTAAGGAAATTATTTATCCGAAGGGTGGTGAGATTGATATCCGGGCAATTGTGGTTGGTGATTACACAATGTCTGTCTTGGAGATATGGGGCGCAGAATATCAGGAACAAGATGCCATCTTGGTTAAACCTGAAAGTCGCGAACTTTTGCAATCAATATGTGAAAGAGAAAGGTGTTCTATGGCTGTTATTGGAACAATAAATGGTGAGGGACGTATTGTTTTAATTGATAGCTTAGCTATTGAAAAATCTAAATCAAGTGGCCTCCCTCCACCTGATCCTGCTGTCGATTTGGAGCTTGAGAAAGTACTTGGTGATATGCCACAAAAAAGCTTTAAATTCGAGCGGATGGCAGATGCACGAGAGCCACTTGATATTGCCCCTGGAATCACAGTAATGGATGCTCTTAAGAGAGTTTTAAGACTTCCATCCATCTGTTCAAAGCGCTTCTTGACCTCGAAAGTAGACAGGTGTGTTACAGGTCTGGTAGCTCAGCAGCAAACTGTCGGGCCCCTGCAAATTCCTCTCTCTGATGTTGGAGTAATTGCTCAGACTTTTACCGGCTTGACTGGAGGTGCATGTGCCATTGGGGAGCAGCCAATCAAAGGTTTGTTGGATCCAAAAGCAATGGCTAGATTGGCAGTTGGAGAAGCACTCACAAATCTTGTTTGGGCTAAGGTCACTTCTCTATCTGATGTTAAAGCAAGTGGGAATTGGATGTATGCAGCCAAGCTTGATGGTGAAGGAGCAGCCATGTATGATGCTGCTAATGCTCTTTCAGAGGCGATGATCAAACTTGGTATTGCTATTGATGGGGGAAAGGACAGTCTTTCCATGGCAGCCCATGCTGCAGGAGAAGTTGTTAAGGCTCCTGGAAATCTTGTAATCAGTGTTTATTGTACTTGTCCTGACATAACAAAAACAGTAACCCCAGATTTGAAGCTTGGAGATGATGGTGTTCTGCTTCACATTGATTTAGCCGCAGGAAAGCGGCGATTAGGTGGCTCTGCTCTTGCTCAGGTTTTTGACCAGATTGGGAACGATTGTCCTGACCTTGAGGATGTTCCGTATCTGAAGCGAGTGTTTGAGGGAGTTCAAGGCCTTCTTGATGATGAACTGATCTCTGCTGGCCATGATATCAGTGATGGTGGGCTTCTGGTCTGTGCCCTTGAGATGGCATTTGCTGGCAATCGTGGCATTAATTTGGACTTGACTACGCACCGGAAAAGCCTCTTCCAAACAATTTTTTCTGAAGAGCTTGGTCTGGTTATTGAGGTCAGCAAGAAAAACTTAGATATGGTAATAGGAAAGCTAAGCAGTGGAGGTATTTCTGCAGAGATTATTGGACAGGTAACTGCCACACCTTCAATAGTGTTAAAGGTGGATGGGGTAACTCATTTGAACGAAAGTACTTCTTTTGTAAGGGACATGTGGGAGGATACTAGTTTTCAGCTGGAAAAATTTCAGAGATTGGCTTCTTGTGTTGATCTAGAGAAAGAAGGGTTGAAAGATAGGCATGAGCCTTCATGGCAGTTGTCTTTTACTCCATCCTTCACAGATGAAAAGTATATGACCACGACCTCCAAACCGAAGGTGGCTGTTATTCGAGAAGAAGGAAGCAATGGGGATAGAGAAATGGCTGCGGCATTTTATGCTGCTGGCTTTGAGCCATGGGATATTACAATGTCAGACCTTCTTAATGGAGCTGTATCACTGCAAGAGTTCCGTGGAATTGTGTTTGTTGGAGGTTTTAGCTATGCTGATGTCCTTGATTCTGCAAAAGGTTGGTCTGCTTCAATTAGATTTAATCAGCCCCTTCTAAATCAATTTCAGGAGTTTTACAAGAGGCACGACACTTTCAGTCTTGGCGTTTGCAATGGGTGTCAGCTTATGGCTCTTTTGGGCTGGGTCCCAGGTCCTCAAGTTGGGGGTGTGCATGGTGGTGGTGGGGATCCATCACAGCCGAGGTTCATTCACAATGAGTCAGGCCGGTTTGAGTGTCGCTTTACTAGTGTAAAAATTGAGGACTCACCTTCTATAATGTTAAAAGGAATGGAAGGCAGTACTTTGGGCGTGTGGGCTGCCCATGGTGAGGGAAGAGCCTATTTTCCTGATGATGGTGTTTTTGATCGTGTGCTCCACTCCAAGTTGGCTCCAGTAAGATATTGTGATGATGATGGGATTGGAACGGAACTTTATCCCTTCAACCTGAATGGCTCTCCCTTGGGAGTTGCGGCAATTTGTTCACCAGATGGGAGGCATCTTGCCATGATGCCTCATCCAGAGCGCTGCTTCTTAATGTGGCAGTACCCATGGTATCCCAAGCAGTGGGATGTTGAAAAGAAAGGCCCTAGTCCATGGCTACGGATGTTCCAGAATGCCAGAGAGTGGTGCTCCAGAAATGATGCCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0003674 GO:0003824 GO:0004642 GO:0005488 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0007275 GO:0008144 GO:0008150 GO:0009507 GO:0009532 GO:0009536 GO:0009555 GO:0009570 GO:0016874 GO:0016879 GO:0016884 GO:0017076 GO:0030554 GO:0032501 GO:0032502 GO:0032553 GO:0032555 GO:0032559 GO:0035639 GO:0036094 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0048229 GO:0048856 GO:0055046 GO:0097159 GO:0097367 GO:1901265 GO:1901363

KEGG Pathways

Metabolic & Signaling

ko00230 ko01100 ko01110 ko01130 map00230 map01100 map01110 map01130

Pfam Domains

Protein Families

AIRS_C GATase_5

Protein Analysis

1412

Amino Acids

154.24

Weight (kDa)

5.57

Isoelectric Point (pI)

37.68

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FGAR-AT_N	PF18076	125 - 242	9.4e-21	Formylglycinamide ribonucleotide amidotransferase N-terminal
FGAR-AT_linker	PF18072	268 - 317	4.8e-11	Formylglycinamide ribonucleotide amidotransferase linker domain
AIRS_C	PF02769	531 - 685	1.9e-27	AIR synthase related protein, C-terminal domain
FGAR-AT_PurM_N-like	PF22689	756 - 909	2.1e-52	FGAR-AT PurM_N-like domain
AIRS_C	PF02769	940 - 1070	7.4e-18	AIR synthase related protein, C-terminal domain
GATase_5	PF13507	1138 - 1407	3.8e-105	CobB/CobQ-like glutamine amidotransferase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0005762)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G74260
fragaria_vesca	FvH4_5g19530 FvH4_5g19530 FvH4_5g19530
malus_domestica	MD03G1203100.v1.1 MD04G1022300.v1.1
prunus_persica	Prupe.1G542100_v2.0.a1 Prupe.1G542100_v2.0.a1 Prupe.1G542100_v2.0.a1 Prupe.1G542100_v2.0.a1 Prupe.1G542100_v2.0.a1 Prupe.8G024000_v2.0.a1 Prupe.8G024000_v2.0.a1 Prupe.8G024000_v2.0.a1
pyrus_communis	pycom04g01890 pycom04g01930
rosa_chinensis	RchiOBHm_Chr7g0204141
rosa_laevigata	RLG00000003485
rosa_multiflora	Rmu_sc0001311.1_g000031
rosa_roxburghii	Rroxscaffold_3G00253310
rosa_rugosa	Rorug07G0081100 Rorug07G0081200 Rorug07G0081200
rosa_samantha	Rh7AG211000 Rh7BG209600 Rh7CG223500 Rh7DG217800
rosa_wichuraiana	Rw7G018360

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	2 cut(s)	4155, 4215
AccBSI	CCGCTC	1 cut(s)	2173
AccI	GTMKAC	2 cut(s)	468, 2292
AccII	CGCG	1 cut(s)	1968
AciI	CCGC	9 cut(s)	130, 889, 1507, 1682, 2173, 2808, 2818, 3470, 4061
AclWI	GGATC	9 cut(s)	666, 1339, 1346, 1390, 2099, 2438, 2451, 3773, 3786
AcoI	YGGCCR	3 cut(s)	46, 1669, 2956
AcsI	RAATTY	8 cut(s)	32, 457, 969, 1375, 1439, 2165, 2346, 3287
AcuI	CTGAAG	3 cut(s)	419, 2921, 3099
AcvI	CACGTG	1 cut(s)	1154
AfaI	GTAC	8 cut(s)	629, 765, 1704, 2136, 2728, 3238, 3892, 4136
AfeI	AGCGCT	2 cut(s)	2273, 4115
AflII	CTTAAG	1 cut(s)	2234
AflIII	ACRYGT	2 cut(s)	1143, 3255
AgeI	ACCGGT	1 cut(s)	1111
AhlI	ACTAGT	2 cut(s)	3269, 3836
AjnI	CCWGG	6 cut(s)	863, 1120, 1216, 2209, 2698, 3739
AleI	CACNNNNGTG	1 cut(s)	75
Alw21I	GWGCWC	4 cut(s)	175, 1025, 3966, 4223
Alw26I	GTCTC	6 cut(s)	434, 574, 1175, 1220, 1245, 1480
AlwI	GGATC	9 cut(s)	666, 1339, 1346, 1390, 2099, 2438, 2451, 3773, 3786
AlwNI	CAGNNNCTG	3 cut(s)	61, 305, 951
Ama87I	CYCGRG	1 cut(s)	1072
Aor51HI	AGCGCT	2 cut(s)	2273, 4115
ApaI	GGGCCC	1 cut(s)	2340
ApoI	RAATTY	8 cut(s)	32, 457, 969, 1375, 1439, 2165, 2346, 3287
ArsI	GACNNNNNNTTYG	2 cut(s)	3599, 3631
AseI	ATTAAT	3 cut(s)	669, 813, 3027
AsiGI	ACCGGT	1 cut(s)	1111
Asp700I	GAANNNNTTC	2 cut(s)	154, 3288
AspLEI	GCGC	4 cut(s)	226, 1922, 2274, 4116
AspS9I	GGNCC	8 cut(s)	58, 527, 1616, 2336, 2337, 3736, 3743, 4175
AsuC2I	CCSGG	1 cut(s)	1871
AvaI	CYCGRG	1 cut(s)	1072
AvaII	GGWCC	3 cut(s)	527, 3736, 3743
BaeGI	GKGCMC	3 cut(s)	113, 2340, 2995
BalI	TGGCCA	3 cut(s)	48, 1671, 2958
BamHI	GGATCC	2 cut(s)	2443, 3778
BanII	GRGCYC	4 cut(s)	387, 1277, 1452, 2340
BauI	CACGAG	1 cut(s)	2187
BbrPI	CACGTG	1 cut(s)	1154
BbsI	GAAGAC	1 cut(s)	549
Bbv12I	GWGCWC	4 cut(s)	175, 1025, 3966, 4223
BceAI	ACGGC	1 cut(s)	1447
BciT130I	CCWGG	6 cut(s)	865, 1122, 1218, 2211, 2700, 3741
BciVI	GTATCC	3 cut(s)	1377, 3259, 4155
BclI	TGATCA	2 cut(s)	1555, 2616
BcnI	CCSGG	1 cut(s)	1871
BcoDI	GTCTC	6 cut(s)	434, 574, 1175, 1220, 1245, 1480
BcuI	ACTAGT	2 cut(s)	3269, 3836
BfaI	CTAG	9 cut(s)	402, 1410, 1470, 1598, 2460, 3270, 3317, 3837, 4179
BfmI	CTRYAG	4 cut(s)	1176, 1203, 2676, 3159
BfoI	RGCGCY	3 cut(s)	227, 2275, 4117
BfrI	CTTAAG	1 cut(s)	2234
BfuI	GTATCC	3 cut(s)	1377, 3259, 4155
BglI	GCCNNNNNGGC	1 cut(s)	3355
BglII	AGATCT	1 cut(s)	847
BlpI	GCTNAGC	3 cut(s)	2062, 2319, 3140
BmcAI	AGTACT	3 cut(s)	2136, 3238, 3892
Bme1390I	CCNGG	7 cut(s)	865, 1122, 1218, 1871, 2211, 2700, 3741
Bme18I	GGWCC	3 cut(s)	527, 3736, 3743
BmeT110I	CYCGRG	1 cut(s)	1072
BmgT120I	GGNCC	8 cut(s)	58, 527, 1616, 2336, 2337, 3736, 3743, 4175
BmiI	GGNNCC	9 cut(s)	753, 2338, 2339, 2445, 2697, 3737, 3738, 3780, 3981
BmrFI	CCNGG	7 cut(s)	865, 1122, 1218, 1871, 2211, 2700, 3741
BpiI	GAAGAC	1 cut(s)	549
BplI	GAGNNNNNCTC	4 cut(s)	2466, 2498, 4205, 4237
BpmI	CTGGAG	3 cut(s)	2418, 3966, 4207
Bpu10I	CCTNAGC	2 cut(s)	2502, 2844
Bpu1102I	GCTNAGC	3 cut(s)	2062, 2319, 3140
BpuEI	CTTGAG	5 cut(s)	159, 2147, 2903, 3017, 3732
BpuMI	CCSGG	1 cut(s)	1871
BsaAI	YACGTR	1 cut(s)	1154
BsaBI	GATNNNNATC	1 cut(s)	1865
BsaI	GGTCTC	1 cut(s)	1175
BsaWI	WCCGGW	2 cut(s)	1111, 3048
BsaXI	ACNNNNNCTCC	2 cut(s)	2673, 2703
Bse118I	RCCGGY	3 cut(s)	1111, 2387, 3816
Bse1I	ACTGG	6 cut(s)	431, 956, 967, 1270, 2401, 3983
Bse3DI	GCAATG	5 cut(s)	690, 1136, 2460, 3454, 3709
Bse8I	GATNNNNATC	1 cut(s)	1865
BseBI	CCWGG	6 cut(s)	865, 1122, 1218, 2211, 2700, 3741
BseJI	GATNNNNATC	1 cut(s)	1865
BseMI	GCAATG	5 cut(s)	690, 1136, 2460, 3454, 3709
BseMII	CTCAG	6 cut(s)	270, 297, 354, 2333, 2390, 2858
BseNI	ACTGG	6 cut(s)	431, 956, 967, 1270, 2401, 3983
BseRI	GAGGAG	2 cut(s)	637, 821
BseSI	GKGCMC	3 cut(s)	113, 2340, 2995
BseYI	CCCAGC	2 cut(s)	720, 3732
BsgI	GTGCAG	7 cut(s)	101, 318, 344, 1134, 1242, 1254, 1557
Bsh1236I	CGCG	1 cut(s)	1968
Bsh1285I	CGRYCG	2 cut(s)	620, 1112
BshTI	ACCGGT	1 cut(s)	1111
BsiEI	CGRYCG	2 cut(s)	620, 1112
BsiHKAI	GWGCWC	4 cut(s)	175, 1025, 3966, 4223
BsiHKCI	CYCGRG	1 cut(s)	1072
BsiSI	CCGG	5 cut(s)	1112, 1870, 2388, 3049, 3817
BslFI	GGGAC	4 cut(s)	263, 2052, 3266, 3722
BsmAI	GTCTC	6 cut(s)	434, 574, 1175, 1220, 1245, 1480
BsmFI	GGGAC	4 cut(s)	263, 2052, 3266, 3722
BsmI	GAATGC	2 cut(s)	1791, 4210
Bso31I	GGTCTC	1 cut(s)	1175
BsoBI	CYCGRG	1 cut(s)	1072
Bsp120I	GGGCCC	1 cut(s)	2336
Bsp1407I	TGTACA	1 cut(s)	1702
Bsp1720I	GCTNAGC	3 cut(s)	2062, 2319, 3140
Bsp19I	CCATGG	6 cut(s)	990, 2661, 3497, 3913, 4139, 4184
Bsp68I	TCGCGA	1 cut(s)	1968
BspACI	CCGC	9 cut(s)	130, 889, 1507, 1682, 2173, 2808, 2818, 3470, 4061
BspCNI	CTCAG	6 cut(s)	269, 298, 355, 2332, 2389, 2857
BspFNI	CGCG	1 cut(s)	1968
BspHI	TCATGA	2 cut(s)	691, 1795
BspLI	GGNNCC	9 cut(s)	753, 2338, 2339, 2445, 2697, 3737, 3738, 3780, 3981
BspMAI	CTGCAG	3 cut(s)	1180, 2680, 3163
BspPI	GGATC	9 cut(s)	666, 1339, 1346, 1390, 2099, 2438, 2451, 3773, 3786
BspQI	GCTCTTC	3 cut(s)	1013, 3075, 3919
BspTI	CTTAAG	1 cut(s)	2234
BspTNI	GGTCTC	1 cut(s)	1175
BsrBI	CCGCTC	1 cut(s)	2173
BsrDI	GCAATG	5 cut(s)	690, 1136, 2460, 3454, 3709
BsrFI	RCCGGY	3 cut(s)	1111, 2387, 3816
BsrGI	TGTACA	1 cut(s)	1702
BsrI	ACTGG	6 cut(s)	431, 956, 967, 1270, 2401, 3983
BssAI	RCCGGY	3 cut(s)	1111, 2387, 3816
BssSI	CACGAG	1 cut(s)	2187
BssT1I	CCWWGG	9 cut(s)	990, 1343, 1801, 2661, 3497, 3913, 4050, 4139, 4184
Bst2BI	CACGAG	1 cut(s)	2187
Bst2UI	CCWGG	6 cut(s)	865, 1122, 1218, 2211, 2700, 3741
Bst6I	CTCTTC	6 cut(s)	482, 873, 1013, 3065, 3075, 3919
BstAFI	CTTAAG	1 cut(s)	2234
BstAPI	GCANNNNNTGC	3 cut(s)	1295, 2672, 3479
BstAUI	TGTACA	1 cut(s)	1702
BstBAI	YACGTR	1 cut(s)	1154
BstC8I	GCNNGC	8 cut(s)	113, 947, 1032, 1595, 1756, 2956, 3013, 3487
BstDSI	CCRYGG	7 cut(s)	990, 2661, 3497, 3553, 3913, 4139, 4184
BstFNI	CGCG	1 cut(s)	1968
BstH2I	RGCGCY	3 cut(s)	227, 2275, 4117
BstHHI	GCGC	4 cut(s)	226, 1922, 2274, 4116
BstMAI	GTCTC	6 cut(s)	434, 574, 1175, 1220, 1245, 1480
BstMCI	CGRYCG	2 cut(s)	620, 1112
BstNI	CCWGG	6 cut(s)	865, 1122, 1218, 2211, 2700, 3741
BstNSI	RCATGY	4 cut(s)	1147, 1597, 2409, 3259
BstSCI	CCNGG	7 cut(s)	863, 1120, 1216, 1869, 2209, 2698, 3739
BstSFI	CTRYAG	4 cut(s)	1176, 1203, 2676, 3159
BstSLI	GKGCMC	3 cut(s)	113, 2340, 2995
BstUI	CGCG	1 cut(s)	1968
BstV2I	GAAGAC	1 cut(s)	549
BstX2I	RGATCY	6 cut(s)	847, 1331, 1351, 1395, 2443, 3778
BstXI	CCANNNNNNTGG	3 cut(s)	350, 1265, 1808
BstYI	RGATCY	6 cut(s)	847, 1331, 1351, 1395, 2443, 3778
BsuI	GTATCC	3 cut(s)	1377, 3259, 4155
BtgI	CCRYGG	7 cut(s)	990, 2661, 3497, 3553, 3913, 4139, 4184
BtgZI	GCGATG	1 cut(s)	2627
BtsI	GCAGTG	5 cut(s)	21, 374, 3151, 3539, 4160
BtuMI	TCGCGA	1 cut(s)	1968
Cac8I	GCNNGC	8 cut(s)	113, 947, 1032, 1595, 1756, 2956, 3013, 3487
CaiI	CAGNNNCTG	3 cut(s)	61, 305, 951
CciI	TCATGA	2 cut(s)	691, 1795
CfoI	GCGC	4 cut(s)	226, 1922, 2274, 4116
Cfr10I	RCCGGY	3 cut(s)	1111, 2387, 3816
Cfr13I	GGNCC	8 cut(s)	58, 527, 1616, 2336, 2337, 3736, 3743, 4175
CseI	GACGC	1 cut(s)	291
Csp6I	GTAC	8 cut(s)	628, 764, 1703, 2135, 2727, 3237, 3891, 4135
CspAI	ACCGGT	1 cut(s)	1111
CspCI	CAANNNNNGTGG	2 cut(s)	175, 210
CviQI	GTAC	8 cut(s)	628, 764, 1703, 2135, 2727, 3237, 3891, 4135
DraI	TTTAAA	1 cut(s)	2164
EaeI	YGGCCR	3 cut(s)	46, 1669, 2956
Eam1104I	CTCTTC	6 cut(s)	482, 873, 1013, 3065, 3075, 3919
EarI	CTCTTC	6 cut(s)	482, 873, 1013, 3065, 3075, 3919
Eco130I	CCWWGG	9 cut(s)	990, 1343, 1801, 2661, 3497, 3913, 4050, 4139, 4184
Eco147I	AGGCCT	1 cut(s)	2928
Eco24I	GRGCYC	4 cut(s)	387, 1277, 1452, 2340
Eco31I	GGTCTC	1 cut(s)	1175
Eco32I	GATATC	2 cut(s)	1867, 2965
Eco47I	GGWCC	3 cut(s)	527, 3736, 3743
Eco47III	AGCGCT	2 cut(s)	2273, 4115
Eco57I	CTGAAG	3 cut(s)	419, 2921, 3099
Eco72I	CACGTG	1 cut(s)	1154
Eco88I	CYCGRG	1 cut(s)	1072
EcoO109I	RGGNCCY	6 cut(s)	58, 1616, 2337, 3736, 3743, 4175
EcoRI	GAATTC	1 cut(s)	32
EcoRII	CCWGG	6 cut(s)	863, 1120, 1216, 2209, 2698, 3739
EcoRV	GATATC	2 cut(s)	1867, 2965
EcoT14I	CCWWGG	9 cut(s)	990, 1343, 1801, 2661, 3497, 3913, 4050, 4139, 4184
EcoT38I	GRGCYC	4 cut(s)	387, 1277, 1452, 2340
ErhI	CCWWGG	9 cut(s)	990, 1343, 1801, 2661, 3497, 3913, 4050, 4139, 4184
FaqI	GGGAC	4 cut(s)	263, 2052, 3266, 3722
FauI	CCCGC	2 cut(s)	123, 1500
FbaI	TGATCA	2 cut(s)	1555, 2616
FblI	GTMKAC	2 cut(s)	468, 2292
FriOI	GRGCYC	4 cut(s)	387, 1277, 1452, 2340
FspBI	CTAG	9 cut(s)	402, 1410, 1470, 1598, 2460, 3270, 3317, 3837, 4179
GlaI	GCGC	4 cut(s)	225, 1921, 2273, 4115
GsaI	CCCAGC	2 cut(s)	724, 3736
GsuI	CTGGAG	3 cut(s)	2418, 3966, 4207
HaeII	RGCGCY	3 cut(s)	227, 2275, 4117
HapII	CCGG	5 cut(s)	1112, 1870, 2388, 3049, 3817
HgaI	GACGC	1 cut(s)	291
HhaI	GCGC	4 cut(s)	226, 1922, 2274, 4116
Hin6I	GCGC	4 cut(s)	224, 1920, 2272, 4114
HinP1I	GCGC	4 cut(s)	224, 1920, 2272, 4114
HincII	GTYRAC	1 cut(s)	469
HindII	GTYRAC	1 cut(s)	469
HindIII	AAGCTT	4 cut(s)	410, 2158, 2558, 2768
HinfI	GANTC	9 cut(s)	604, 892, 1245, 1456, 1488, 2214, 3599, 3809, 3854
HpaII	CCGG	5 cut(s)	1112, 1870, 2388, 3049, 3817
Hpy166II	GTNNAC	5 cut(s)	469, 543, 715, 2293, 4073
Hpy8I	GTNNAC	5 cut(s)	469, 543, 715, 2293, 4073
HpyCH4IV	ACGT	3 cut(s)	1153, 1824, 2041
HpySE526I	ACGT	3 cut(s)	1153, 1824, 2041
HspAI	GCGC	4 cut(s)	224, 1920, 2272, 4114
KflI	GGGWCCC	1 cut(s)	3736
Ksp22I	TGATCA	2 cut(s)	1555, 2616
LguI	GCTCTTC	3 cut(s)	1013, 3075, 3919
MaeI	CTAG	9 cut(s)	402, 1410, 1470, 1598, 2460, 3270, 3317, 3837, 4179
MaeII	ACGT	3 cut(s)	1153, 1824, 2041
MbiI	CCGCTC	1 cut(s)	2173
MfeI	CAATTG	2 cut(s)	1103, 1875
MflI	RGATCY	6 cut(s)	847, 1331, 1351, 1395, 2443, 3778
MlsI	TGGCCA	3 cut(s)	48, 1671, 2958
MluNI	TGGCCA	3 cut(s)	48, 1671, 2958
MlyI	GAGTC	4 cut(s)	598, 1482, 3818, 3848
MmeI	TCCRAC	8 cut(s)	773, 920, 954, 1291, 2344, 2421, 2452, 3550
Mox20I	TGGCCA	3 cut(s)	48, 1671, 2958
MroXI	GAANNNNTTC	2 cut(s)	154, 3288
MscI	TGGCCA	3 cut(s)	48, 1671, 2958
MslI	CAYNNNNRTG	5 cut(s)	75, 163, 1763, 2222, 3768
Msp20I	TGGCCA	3 cut(s)	48, 1671, 2958
MspA1I	CMGCKG	2 cut(s)	191, 3280
MspCI	CTTAAG	1 cut(s)	2234
MspI	CCGG	5 cut(s)	1112, 1870, 2388, 3049, 3817
MspR9I	CCNGG	7 cut(s)	865, 1122, 1218, 1871, 2211, 2700, 3741
MunI	CAATTG	2 cut(s)	1103, 1875
Mva1269I	GAATGC	2 cut(s)	1791, 4210
MvaI	CCWGG	6 cut(s)	865, 1122, 1218, 2211, 2700, 3741
MvnI	CGCG	1 cut(s)	1968
NciI	CCSGG	1 cut(s)	1871
NcoI	CCATGG	6 cut(s)	990, 2661, 3497, 3913, 4139, 4184
NlaIV	GGNNCC	9 cut(s)	753, 2338, 2339, 2445, 2697, 3737, 3738, 3780, 3981
NmeAIII	GCCGAG	1 cut(s)	3816
NmuCI	GTSAC	4 cut(s)	404, 601, 1154, 2506
NruI	TCGCGA	1 cut(s)	1968
NspI	RCATGY	4 cut(s)	1147, 1597, 2409, 3259
OliI	CACNNNNGTG	1 cut(s)	75
PaeI	GCATGC	1 cut(s)	1597
PaeR7I	CTCGAG	1 cut(s)	1072
PagI	TCATGA	2 cut(s)	691, 1795
PceI	AGGCCT	1 cut(s)	2928
PciI	ACATGT	2 cut(s)	1143, 3255
PciSI	GCTCTTC	3 cut(s)	1013, 3075, 3919
PcsI	WCGNNNNNNNCGW	1 cut(s)	1747
PctI	GAATGC	2 cut(s)	1791, 4210
PdmI	GAANNNNTTC	2 cut(s)	154, 3288
PfeI	GAWTC	5 cut(s)	892, 1245, 1456, 2214, 3599
PflMI	CCANNNNNTGG	2 cut(s)	4155, 4215
PfoI	TCCNGGA	1 cut(s)	2698
PinAI	ACCGGT	1 cut(s)	1111
PleI	GAGTC	4 cut(s)	598, 1482, 3817, 3848
PmaCI	CACGTG	1 cut(s)	1154
PmlI	CACGTG	1 cut(s)	1154
PpsI	GAGTC	4 cut(s)	598, 1482, 3817, 3848
Ppu21I	YACGTR	1 cut(s)	1154
PpuMI	RGGWCCY	2 cut(s)	3736, 3743
PscI	ACATGT	2 cut(s)	1143, 3255
PshBI	ATTAAT	3 cut(s)	669, 813, 3027
Psp5II	RGGWCCY	2 cut(s)	3736, 3743
Psp6I	CCWGG	6 cut(s)	863, 1120, 1216, 2209, 2698, 3739
PspCI	CACGTG	1 cut(s)	1154
PspFI	CCCAGC	2 cut(s)	720, 3732
PspGI	CCWGG	6 cut(s)	863, 1120, 1216, 2209, 2698, 3739
PspN4I	GGNNCC	9 cut(s)	753, 2338, 2339, 2445, 2697, 3737, 3738, 3780, 3981
PspOMI	GGGCCC	1 cut(s)	2336
PspPI	GGNCC	8 cut(s)	58, 527, 1616, 2336, 2337, 3736, 3743, 4175
PspPPI	RGGWCCY	2 cut(s)	3736, 3743
PspXI	VCTCGAGB	1 cut(s)	1072
PstI	CTGCAG	3 cut(s)	1180, 2680, 3163
PstNI	CAGNNNCTG	3 cut(s)	61, 305, 951
PsuI	RGATCY	6 cut(s)	847, 1331, 1351, 1395, 2443, 3778
PvuII	CAGCTG	2 cut(s)	191, 3280
RruI	TCGCGA	1 cut(s)	1968
RsaI	GTAC	8 cut(s)	629, 765, 1704, 2136, 2728, 3238, 3892, 4136
RsaNI	GTAC	8 cut(s)	628, 764, 1703, 2135, 2727, 3237, 3891, 4135
RseI	CAYNNNNRTG	5 cut(s)	75, 163, 1763, 2222, 3768
SalI	GTCGAC	1 cut(s)	467
SapI	GCTCTTC	3 cut(s)	1013, 3075, 3919
Sau96I	GGNCC	8 cut(s)	58, 527, 1616, 2336, 2337, 3736, 3743, 4175
ScaI	AGTACT	3 cut(s)	2136, 3238, 3892
SchI	GAGTC	4 cut(s)	598, 1482, 3818, 3848
ScrFI	CCNGG	7 cut(s)	865, 1122, 1218, 1871, 2211, 2700, 3741
SfcI	CTRYAG	4 cut(s)	1176, 1203, 2676, 3159
Sfr274I	CTCGAG	1 cut(s)	1072
SinI	GGWCC	3 cut(s)	527, 3736, 3743
SlaI	CTCGAG	1 cut(s)	1072
SmiMI	CAYNNNNRTG	5 cut(s)	75, 163, 1763, 2222, 3768
SmlI	CTYRAG	7 cut(s)	174, 1072, 2126, 2234, 2882, 2996, 3747
SmoI	CTYRAG	7 cut(s)	174, 1072, 2126, 2234, 2882, 2996, 3747
SpeI	ACTAGT	2 cut(s)	3269, 3836
SphI	GCATGC	1 cut(s)	1597
SseBI	AGGCCT	1 cut(s)	2928
SsiI	CCGC	9 cut(s)	130, 889, 1507, 1682, 2173, 2808, 2818, 3470, 4061
SspMI	CTAG	9 cut(s)	402, 1410, 1470, 1598, 2460, 3270, 3317, 3837, 4179
StuI	AGGCCT	1 cut(s)	2928
StyD4I	CCNGG	7 cut(s)	863, 1120, 1216, 1869, 2209, 2698, 3739
StyI	CCWWGG	9 cut(s)	990, 1343, 1801, 2661, 3497, 3913, 4050, 4139, 4184
TaiI	ACGT	3 cut(s)	1156, 1827, 2044
TatI	WGTACW	5 cut(s)	1702, 2134, 2726, 3236, 3890
TauI	GCSGC	4 cut(s)	2810, 2821, 3473, 4064
TfiI	GAWTC	5 cut(s)	892, 1245, 1456, 2214, 3599
TseFI	GTSAC	4 cut(s)	404, 601, 1154, 2506
Tsp45I	GTSAC	4 cut(s)	404, 601, 1154, 2506
TspDTI	ATGAA	8 cut(s)	25, 147, 680, 1784, 2955, 3342, 3402, 3787
TspGWI	ACGGA	6 cut(s)	719, 1344, 2883, 3542, 4031, 4207
Van91I	CCANNNNNTGG	2 cut(s)	4155, 4215
Vha464I	CTTAAG	1 cut(s)	2234
VpaK11BI	GGWCC	3 cut(s)	527, 3736, 3743
VspI	ATTAAT	3 cut(s)	669, 813, 3027
XapI	RAATTY	8 cut(s)	32, 457, 969, 1375, 1439, 2165, 2346, 3287
XbaI	TCTAGA	1 cut(s)	3316
XceI	RCATGY	4 cut(s)	1147, 1597, 2409, 3259
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1808, 3418
XhoI	CTCGAG	1 cut(s)	1072
XmiI	GTMKAC	2 cut(s)	468, 2292
XmnI	GAANNNNTTC	2 cut(s)	154, 3288
XspI	CTAG	9 cut(s)	402, 1410, 1470, 1598, 2460, 3270, 3317, 3837, 4179
ZrmI	AGTACT	3 cut(s)	2136, 3238, 3892

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.