Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0005762

Physical Location & Seq

Genomic Coordinates Forward (+)

2616569 .. 2629760

Gene Structure 13192 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1022300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4221 bp

ATGTTGTTTGGTTTTATTTGGCAGGGCACGAATAGGCAGAGCCTTTTTTTGCAGAGGAACTCTGTTAAAGGAAGAAGCCAGGTGCTTTGGGGCTCGCTTCACGGGCGAAGTTCAGCTCCCAGTTTTGGTAATAGGAGAGGTGTTTCTTTGAGGTGTCGTGCTCAAGAAAAGCCCAGAGCTGTGGTTTCTGGTGGTGTGAGCAGCTTGGTAGATGATGAGCAGTCAAGCTTGGTTGAGAAGCCTGCAGCTGAAGTTATTCATTTTTTCCGCATCCCGCTCATTCAGGAGAGTGCAACAGCTGAGCTTCTCAAGACGGTTCAGACGAAGATTACGAATCAGATTGTTGGTTTGAAAACTGAGCAGTGCTTCAACATTGGCCTTGACTCGCCGCTTTCCAGCGACAAGGTTTTGGTTTTGAAGTGGCTTCTTCAGGAGACTTATGAGCCCGAGAATTTGGGGACTGAGAGCTTTCTTGAAAAGAAAAGGCAGGAAGGGCTTAGTACAGCTATTGTTGAGGTTGGTCCTAGGTTGTCATTTACAACAGCCTGGTCTTCTAATGCTGTATCGATTTGTCGAGCATGTGGGTTGACAGAGGTGACTCGTTTGGAACGGTCAAGGAGGTACTTGCTATTCAGTAAGGGATCACTTCAAGATCATCAGATTAATGAGTTTGCTGCACTGGTTCATGATCGAATGACTGAGTGTGTGTATGCCCATAAGCTAGTTTCATTTGAAACGAGTGTAGTTCCGGATGAAGTTCGTCATGTAAATGTCATGGAGAGGGGTCGGAAGGCATTGGAGGAGATAAATCAGGAAATGGGTTTGGCATTTGATGAGCAAGATCTCCAGTACTACACTAGGCTATTCAAGGACGAAATCCAGAGGAATCCAACAACAGTTGAGCTGTTTGATATTGCACAATCCAACAGTGAGCATAGTAGGCATTGGTTTTTTACTGGCAAAATTATTATAGATGGACAGCCTATGGATAGAACTCTGATGCAGATTGTGAAGCGCACGTTGCAGGCAAACCCAAACAATTCTGTAATTGGCTTCAAGGATAACTCAAGTGCAATCAAGGGGTTTCTTGTGAAGCAAATGCGACCGGTTCAGCCAGGTTCAACATGTCCATTGAGCATAGCTACTCGGGACCTTGATATTTTATTTACTGCTGAGACCCATAATTTCCCATGTGCTGTAGCGCCTTACCCTGGTGCAGAGACAGGCGCAGGAGGTCGAATCAGGGATACCCATGCGACTGGAAGAGGGTCTTTTGTTGTAGCATCTACAGCTGGTTATTGTGTTGGGAATCTCAATATGGAGGGCTCTTATGCACCTTGGGAAGATCCGTCTTTTGCTTATCCATCAAATTTGGCTCCACCTCTGCAGATCCTTATAGATGCTAGCAATGGTGCATCAGACTACGGGAACAAATTTGGTGAGCCCTTGATTCAGGGCTACACTAGAACATTTGGAATGAGACTTCCAAGTGGAGACAGGCGAGAATGGTTGAAGCCAATCATGTTTAGTGGAGGTATTGGGCAGATTGATCACACCCATATAACAAAAGGTGAGCCTGACATTGGTATGCTGGTTGTTAAAATTGGAGGCCCTGCTTATCGTATTGGAATGGGGGGTGGGGCAGCATCGAGCATGGTTAGTGGCCAGAATGATGCGGAGCTTGATTTTAATGCTGTACAGCGTGGAGATGCAGAGATGGCACAGAAACTTTATCGTGTGGTCCGTGCCTGTATTGAGATGGGGGAGAATAACCCTATCATTAGCATTCATGATCAGGGGGCAGGTGGAAACTGCAATGTTGTTAAGGAAATTATATACCCCAAGGGTGGCCAGATTGATATCCGGGCAATTGTAGTTGGTGATCACACAATGTCTGTCTTGGAGATATGGGGTGCAGAATACCAGGAGCAAGATGCAATCTTGGTGAAGCCTGAAAGCCGCCACCTCTTGCAATCAATATGTGAAAGAGAAAGGGTTTCTATGGCTGTTATTGGAACAATAAATGGTGAGGGACGTGCTGTTTTAATTGATAGCTTAGCTATTAAAAAATGTGAATCTAGTGGAATCCCTCCCCCTCCTCCTGCAGTCGATTTGGAGCTTGAGAAAGTACTTGGTGATATGCCACAGAAAAGTTTTGAATTCCACAGGACGACTGATGCACGTGAACCACTTGATATTGCTCCTGGAATAACAGTGATGGATTCACTTAAGAGGGTGTTGAGACTTCCATCTGTTTGTTCAAAGCGCTTCTTGACATCCAAAGTGGACAGGTGTGTTACAGGTCTTGTAGCACAGCAGCAGACTGTTGGGCCCTTGCAAATTCCTCTCTCTGATGTTGCAGTAATAGCACAGACTTTTACTGACATGACTGGAGGTGCATGTGCCATTGGGGAGCAGCCAATCAAAGGTTTGTTGGATCCAAAAGCAATGGCGAGATTGGCTGTTGGAGAAGCACTCACAAATCTTGTTTGGGCAAAGGTCACTTCTCTATCTGATGTTAAAGCCAGCGGGAATTGGATGTATGCAGCCAAGCTTGATGGGGAAGGAGCAGCTATGTATGATGCTGCTACAGCTCTTTCAGAGGCAATGATCGAACTTGGTATTGCAATTGATGGCGGGAAGGACAGTCTTTCCATGGCAGCCCATGTTGCAGGCGAGGTTGTTAAGGCACCTGGCAATCTTGTAATGAGTGTTTATTGCACTTGTCCTGACATAACAAAAACAGTAACCCCAGATTTAAAGCTTAAGGATGATGGCGTGCTGCTTCACATTGATTTGGCAAAGGGAAAGCGGAGGTTAGGTGGGTCTGCTCTTGCTCAGGTTTTTGACCAAATTGGTAATGATTGCCCTGACATTGAGGATGTTCCTTATCTAAAACGAGTTTTTGAGGGAGTTCAGGACCTTCTTTCTGACGAATTGATCTCTGCTGGTCATGATATTAGTGATGGTGGGTTACTGGTCTGTGCCCTTGAAATGGCATTTTCTGGGAATTGTGGCCTTACTTTGGACTTGACTTCACGTGGGAAGAGCCTATTCCAAACGCTTTTTGCTGAAGAACTTGGTCTGGTTATTGAGGTAAGCCGGAATAACTTGGATTTGGTGTTGGAAAAACTGAGCAGTAACAGCATTTTGGCTGAGATTATTGGTCAAGTTAGTGCCACACCCTCAGTAGGGTTAAAGGTTGATGGGGTTACACATTTGCATGAGAGTACTTCGTTTCTAAGGGACTTGTGGGAGGACACTAGTTTTCAGCTGGAAAAATTACAAAGGTTGGCTTCTTGTGTAGATCTAGAAAAAGAAGGGTTGAAAGATAGGCATGAGCCTTCATGGGATTTGTCTTTCACTCCTTCTTTCACAGATGAAAAGTATATGTCCGTCGCTTGCAAACCAAAAGTGGCTATAATTCGAGAAGAAGGCAGCAATGGGGACAGAGAAATGTCGGCAGCATTTTATGCTTCTGGTTTTGAACCATGGGATGTTACAATGTCAGACCTTCTTAATGGAACCATTTCGTTGCATGAATTCCGTGGAATTGCGTTTGTTGGAGGTTTTAGCTACGCTGATGTGCTTGATTCTGCAAAAGGTTGGTCTGCATCGATTCGATTTAATCAGCCCCTTCTGAATCAATTTCAAGAGTTTTACAAGCGGCCAGACACTTTTAGTCTTGGAGTTTGCAACGGGTGTCAGCTTATGGCCCTTTTGGGGTGGGTCCCGGGTCCTCAAGTTGGTGGTGTGCTAGGTGGTGGTGGGGACCCTTCGCAGCCCAGGTTCATTCACAACGAGTCTGGACGGTTTGAATGCCGATTCACCAGTGTGGCAATAAAGGACTCGCCAGCTATAATGTTTAAAGGAATGGAGGGCAGTACATTGGGTGTGTGGGCTGCACATGGTGAAGGAAGAGCATATTTCCCCGACGACGGTGTTCTTGATCGTTTGCTTCACTCAAAGTTGGCACCAGTAAGGTATTGCGATGATGATGGTAATGAGACAGAACTTTACCCTTTCAACGTCAATGGCTCTCCCTTGGGGGTTGCAGCAATTTGTTCTCCAGATGGGAGGCATCTAGCCATGATGCCTCATCCAGAGCGTTGCTTCTTAATGTGGCAGTTCCCTTGGTATCCCAAGCAATGGGATGTGGAAAAGAAAGGCCCTAGTCCCTGGTTGCGAATGTTCCAGAATGCTAGAGAGTGGTGTTCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0003674 GO:0003824 GO:0004642 GO:0005488 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0007275 GO:0008144 GO:0008150 GO:0009507 GO:0009532 GO:0009536 GO:0009555 GO:0009570 GO:0016874 GO:0016879 GO:0016884 GO:0017076 GO:0030554 GO:0032501 GO:0032502 GO:0032553 GO:0032555 GO:0032559 GO:0035639 GO:0036094 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0048229 GO:0048856 GO:0055046 GO:0097159 GO:0097367 GO:1901265 GO:1901363

KEGG Pathways

Metabolic & Signaling

ko00230 ko01100 ko01110 ko01130 map00230 map01100 map01110 map01130

Pfam Domains

Protein Families

AIRS_C GATase_5

Protein Analysis

1407

Amino Acids

153.91

Weight (kDa)

5.42

Isoelectric Point (pI)

38.67

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FGAR-AT_N	PF18076	122 - 239	6.6e-21	Formylglycinamide ribonucleotide amidotransferase N-terminal
FGAR-AT_linker	PF18072	266 - 315	9e-11	Formylglycinamide ribonucleotide amidotransferase linker domain
AIRS_C	PF02769	529 - 683	4.3e-26	AIR synthase related protein, C-terminal domain
FGAR-AT_PurM_N-like	PF22689	754 - 907	1.6e-52	FGAR-AT PurM_N-like domain
AIRS_C	PF02769	938 - 1068	1.3e-17	AIR synthase related protein, C-terminal domain
GATase_5	PF13507	1136 - 1405	2.1e-105	CobB/CobQ-like glutamine amidotransferase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0005762)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G74260
fragaria_vesca	FvH4_5g19530 FvH4_5g19530 FvH4_5g19530
malus_domestica	MD03G1203100.v1.1 MD04G1022300.v1.1
prunus_persica	Prupe.1G542100_v2.0.a1 Prupe.1G542100_v2.0.a1 Prupe.1G542100_v2.0.a1 Prupe.1G542100_v2.0.a1 Prupe.1G542100_v2.0.a1 Prupe.8G024000_v2.0.a1 Prupe.8G024000_v2.0.a1 Prupe.8G024000_v2.0.a1
pyrus_communis	pycom04g01890 pycom04g01930
rosa_chinensis	RchiOBHm_Chr7g0204141
rosa_laevigata	RLG00000003485
rosa_multiflora	Rmu_sc0001311.1_g000031
rosa_roxburghii	Rroxscaffold_3G00253310
rosa_rugosa	Rorug07G0081100 Rorug07G0081200 Rorug07G0081200
rosa_samantha	Rh7AG211000 Rh7BG209600 Rh7CG223500 Rh7DG217800
rosa_wichuraiana	Rw7G018360

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1793
Acc36I	ACCTGC	1 cut(s)	1793
AccB1I	GGYRCC	2 cut(s)	2689, 3973
AccB7I	CCANNNNNTGG	1 cut(s)	4149
AccBSI	CCGCTC	1 cut(s)	277
AccIII	TCCGGA	1 cut(s)	748
AciI	CCGC	9 cut(s)	268, 275, 389, 1676, 1960, 2529, 2637, 2812, 3667
AclWI	GGATC	5 cut(s)	649, 1340, 1384, 2432, 2445
AcoI	YGGCCR	3 cut(s)	1663, 1849, 3668
AcsI	RAATTY	6 cut(s)	451, 1369, 1433, 2159, 2340, 3542
AcuI	CTGAAG	3 cut(s)	270, 413, 3093
AcvI	CACGTG	2 cut(s)	2183, 3041
AdeI	CACNNNGTG	1 cut(s)	3911
AfaI	GTAC	7 cut(s)	502, 623, 851, 1698, 2130, 3232, 3886
AfeI	AGCGCT	1 cut(s)	2267
AflII	CTTAAG	2 cut(s)	2228, 2765
AflIII	ACRYGT	1 cut(s)	1124
AgeI	ACCGGT	1 cut(s)	1105
AhlI	ACTAGT	1 cut(s)	3263
AjiI	CACGTC	1 cut(s)	2036
AjnI	CCWGG	9 cut(s)	78, 545, 1114, 1210, 1923, 2203, 2692, 3785, 4178
AleI	CACNNNNGTG	1 cut(s)	3833
Alw21I	GWGCWC	1 cut(s)	163
Alw26I	GTCTC	7 cut(s)	428, 1169, 1214, 1474, 1488, 2236, 4001
AlwI	GGATC	5 cut(s)	649, 1340, 1384, 2432, 2445
Ama87I	CYCGRG	3 cut(s)	446, 1146, 3733
Aor13HI	TCCGGA	1 cut(s)	748
Aor51HI	AGCGCT	1 cut(s)	2267
AoxI	GGCC	9 cut(s)	376, 1609, 1663, 1849, 2330, 3016, 3668, 3714, 4168
ApaI	GGGCCC	1 cut(s)	2334
ApoI	RAATTY	6 cut(s)	451, 1369, 1433, 2159, 2340, 3542
ArsI	GACNNNNNNTTYG	2 cut(s)	3593, 3625
AseI	ATTAAT	1 cut(s)	663
AsiGI	ACCGGT	1 cut(s)	1105
Asp700I	GAANNNNTTC	2 cut(s)	255, 3529
AspA2I	CCTAGG	1 cut(s)	524
AspLEI	GCGC	4 cut(s)	1017, 1204, 1229, 2268
AsuC2I	CCSGG	3 cut(s)	1865, 3734, 3735
AsuHPI	GGTGA	9 cut(s)	607, 1451, 1583, 1892, 1957, 2039, 2147, 3820, 3923
AsuNHI	GCTAGC	1 cut(s)	1403
AvaI	CYCGRG	3 cut(s)	446, 1146, 3733
AvaII	GGWCC	7 cut(s)	521, 1150, 1741, 2920, 3730, 3737, 3772
AvrII	CCTAGG	1 cut(s)	524
BaeGI	GKGCMC	3 cut(s)	29, 2334, 2989
BalI	TGGCCA	2 cut(s)	1665, 1851
BamHI	GGATCC	1 cut(s)	2437
BanI	GGYRCC	2 cut(s)	2689, 3973
BanII	GRGCYC	5 cut(s)	95, 447, 1328, 1446, 2334
BbrPI	CACGTG	2 cut(s)	2183, 3041
BbsI	GAAGAC	1 cut(s)	543
Bbv12I	GWGCWC	1 cut(s)	163
BciT130I	CCWGG	9 cut(s)	80, 547, 1116, 1212, 1925, 2205, 2694, 3787, 4180
BciVI	GTATCC	2 cut(s)	1240, 4149
BclI	TGATCA	3 cut(s)	1549, 1792, 1882
BcnI	CCSGG	3 cut(s)	1865, 3734, 3735
BcoDI	GTCTC	7 cut(s)	428, 1169, 1214, 1474, 1488, 2236, 4001
BcuI	ACTAGT	1 cut(s)	3263
BfmI	CTRYAG	6 cut(s)	243, 1197, 1287, 1385, 2103, 2589
BfoI	RGCGCY	2 cut(s)	1205, 2269
BfrI	CTTAAG	2 cut(s)	2228, 2765
BfuAI	ACCTGC	1 cut(s)	1793
BfuI	GTATCC	2 cut(s)	1240, 4149
BglII	AGATCT	2 cut(s)	841, 3307
BlnI	CCTAGG	1 cut(s)	524
BlpI	GCTNAGC	2 cut(s)	300, 2056
BmcAI	AGTACT	3 cut(s)	851, 2130, 3232
Bme18I	GGWCC	7 cut(s)	521, 1150, 1741, 2920, 3730, 3737, 3772
BmeT110I	CYCGRG	3 cut(s)	446, 1146, 3733
BmgBI	CACGTC	1 cut(s)	2036
BmrI	ACTGGG	1 cut(s)	114
BmtI	GCTAGC	1 cut(s)	1407
BmuI	ACTGGG	1 cut(s)	114
BpiI	GAAGAC	1 cut(s)	543
BplI	GAGNNNNNCTC	2 cut(s)	2460, 2492
BpmI	CTGGAG	3 cut(s)	830, 2412, 4053
Bpu10I	CCTNAGC	1 cut(s)	2838
Bpu1102I	GCTNAGC	2 cut(s)	300, 2056
BpuEI	CTTGAG	5 cut(s)	147, 293, 1051, 2141, 3726
BpuMI	CCSGG	3 cut(s)	1865, 3734, 3735
Bsa29I	ATCGAT	2 cut(s)	566, 3617
BsaAI	YACGTR	2 cut(s)	2183, 3041
BsaBI	GATNNNNATC	1 cut(s)	1859
BsaI	GGTCTC	1 cut(s)	1169
BsaWI	WCCGGW	2 cut(s)	748, 1105
BsaXI	ACNNNNNCTCC	2 cut(s)	2806, 2836
Bse118I	RCCGGY	1 cut(s)	1105
Bse1I	ACTGG	9 cut(s)	120, 684, 847, 961, 1264, 2395, 2982, 3831, 3977
Bse3DI	GCAATG	6 cut(s)	1414, 1822, 2454, 2613, 3448, 4154
Bse8I	GATNNNNATC	1 cut(s)	1859
BseAI	TCCGGA	1 cut(s)	748
BseBI	CCWGG	9 cut(s)	80, 547, 1116, 1212, 1925, 2205, 2694, 3787, 4180
BseCI	ATCGAT	2 cut(s)	566, 3617
BseGI	GGATG	9 cut(s)	270, 757, 2276, 2544, 2776, 2887, 3502, 4099, 4159
BseJI	GATNNNNATC	1 cut(s)	1859
BseMI	GCAATG	6 cut(s)	1414, 1822, 2454, 2613, 3448, 4154
BseMII	CTCAG	9 cut(s)	291, 348, 453, 690, 1164, 2852, 3125, 3147, 3201
BseNI	ACTGG	9 cut(s)	120, 684, 847, 961, 1264, 2395, 2982, 3831, 3977
BseRI	GAGGAG	2 cut(s)	815, 2088
BseSI	GKGCMC	3 cut(s)	29, 2334, 2989
BsgI	GTGCAG	4 cut(s)	660, 1236, 1935, 3888
Bsh1285I	CGRYCG	1 cut(s)	1106
BshFI	GGCC	9 cut(s)	378, 1611, 1665, 1851, 2332, 3018, 3670, 3716, 4170
BshNI	GGYRCC	2 cut(s)	2689, 3973
BshTI	ACCGGT	1 cut(s)	1105
BshVI	ATCGAT	2 cut(s)	566, 3617
BsiEI	CGRYCG	1 cut(s)	1106
BsiHKAI	GWGCWC	1 cut(s)	163
BsiHKCI	CYCGRG	3 cut(s)	446, 1146, 3733
BsiSI	CCGG	5 cut(s)	749, 1106, 1864, 3103, 3734
BslFI	GGGAC	8 cut(s)	472, 1163, 2046, 3260, 3461, 3716, 3785, 4161
BsmAI	GTCTC	7 cut(s)	428, 1169, 1214, 1474, 1488, 2236, 4001
BsmFI	GGGAC	8 cut(s)	472, 1163, 2046, 3260, 3461, 3716, 3785, 4161
BsmI	GAATGC	3 cut(s)	1785, 3824, 4204
BsnI	GGCC	9 cut(s)	378, 1611, 1665, 1851, 2332, 3018, 3670, 3716, 4170
Bso31I	GGTCTC	1 cut(s)	1169
BsoBI	CYCGRG	3 cut(s)	446, 1146, 3733
Bsp120I	GGGCCC	1 cut(s)	2330
Bsp1286I	GDGCHC	8 cut(s)	29, 95, 163, 447, 1328, 1446, 2334, 2989
Bsp13I	TCCGGA	1 cut(s)	748
Bsp1407I	TGTACA	1 cut(s)	1696
Bsp1720I	GCTNAGC	2 cut(s)	300, 2056
Bsp19I	CCATGG	2 cut(s)	2655, 3491
BspACI	CCGC	9 cut(s)	268, 275, 389, 1676, 1960, 2529, 2637, 2812, 3667
BspANI	GGCC	9 cut(s)	378, 1611, 1665, 1851, 2332, 3018, 3670, 3716, 4170
BspCNI	CTCAG	9 cut(s)	292, 349, 454, 691, 1165, 2851, 3126, 3148, 3200
BspDI	ATCGAT	2 cut(s)	566, 3617
BspEI	TCCGGA	1 cut(s)	748
BspHI	TCATGA	3 cut(s)	685, 1789, 2953
BspMAI	CTGCAG	3 cut(s)	247, 1389, 2107
BspMI	ACCTGC	1 cut(s)	1793
BspOI	GCTAGC	1 cut(s)	1407
BspPI	GGATC	5 cut(s)	649, 1340, 1384, 2432, 2445
BspQI	GCTCTTC	2 cut(s)	3041, 3913
BspT107I	GGYRCC	2 cut(s)	2689, 3973
BspTI	CTTAAG	2 cut(s)	2228, 2765
BspTNI	GGTCTC	1 cut(s)	1169
BsrBI	CCGCTC	1 cut(s)	277
BsrDI	GCAATG	6 cut(s)	1414, 1822, 2454, 2613, 3448, 4154
BsrFI	RCCGGY	1 cut(s)	1105
BsrGI	TGTACA	1 cut(s)	1696
BsrI	ACTGG	9 cut(s)	120, 684, 847, 961, 1264, 2395, 2982, 3831, 3977
BssAI	RCCGGY	1 cut(s)	1105
BssT1I	CCWWGG	7 cut(s)	524, 1337, 1842, 2655, 3491, 4044, 4133
Bst2UI	CCWGG	9 cut(s)	80, 547, 1116, 1212, 1925, 2205, 2694, 3787, 4180
Bst6I	CTCTTC	3 cut(s)	1258, 3041, 3913
BstAFI	CTTAAG	2 cut(s)	2228, 2765
BstAPI	GCANNNNNTGC	1 cut(s)	3473
BstAUI	TGTACA	1 cut(s)	1696
BstBAI	YACGTR	2 cut(s)	2183, 3041
BstC8I	GCNNGC	9 cut(s)	95, 243, 1026, 1405, 2527, 2674, 2780, 3403, 3855
BstDSI	CCRYGG	3 cut(s)	2655, 3491, 3547
BstF5I	GGATG	9 cut(s)	270, 757, 2276, 2544, 2776, 2887, 3502, 4099, 4159
BstH2I	RGCGCY	2 cut(s)	1205, 2269
BstHHI	GCGC	4 cut(s)	1017, 1204, 1229, 2268
BstMAI	GTCTC	7 cut(s)	428, 1169, 1214, 1474, 1488, 2236, 4001
BstMCI	CGRYCG	1 cut(s)	1106
BstNI	CCWGG	9 cut(s)	80, 547, 1116, 1212, 1925, 2205, 2694, 3787, 4180
BstNSI	RCATGY	3 cut(s)	582, 1128, 2403
BstSFI	CTRYAG	6 cut(s)	243, 1197, 1287, 1385, 2103, 2589
BstSLI	GKGCMC	3 cut(s)	29, 2334, 2989
BstV2I	GAAGAC	1 cut(s)	543
BstX2I	RGATCY	5 cut(s)	841, 1345, 1389, 2437, 3307
BstXI	CCANNNNNNTGG	2 cut(s)	181, 1259
BstYI	RGATCY	5 cut(s)	841, 1345, 1389, 2437, 3307
Bsu15I	ATCGAT	2 cut(s)	566, 3617
BsuI	GTATCC	2 cut(s)	1240, 4149
BsuRI	GGCC	9 cut(s)	378, 1611, 1665, 1851, 2332, 3018, 3670, 3716, 4170
BsuTUI	ATCGAT	2 cut(s)	566, 3617
BtgI	CCRYGG	3 cut(s)	2655, 3491, 3547
BtgZI	GCGATG	1 cut(s)	4005
BtrI	CACGTC	1 cut(s)	2036
BtsCI	GGATG	9 cut(s)	270, 757, 2276, 2544, 2776, 2887, 3502, 4099, 4159
BtsI	GCAGTG	1 cut(s)	368
BtsIMutI	CAGTG	5 cut(s)	368, 677, 934, 2220, 3838
BveI	ACCTGC	1 cut(s)	1793
Cac8I	GCNNGC	9 cut(s)	95, 243, 1026, 1405, 2527, 2674, 2780, 3403, 3855
CciI	TCATGA	3 cut(s)	685, 1789, 2953
CfoI	GCGC	4 cut(s)	1017, 1204, 1229, 2268
Cfr10I	RCCGGY	1 cut(s)	1105
Cfr9I	CCCGGG	1 cut(s)	3733
ClaI	ATCGAT	2 cut(s)	566, 3617
Csp6I	GTAC	7 cut(s)	501, 622, 850, 1697, 2129, 3231, 3885
CspAI	ACCGGT	1 cut(s)	1105
CviQI	GTAC	7 cut(s)	501, 622, 850, 1697, 2129, 3231, 3885
DraI	TTTAAA	2 cut(s)	2760, 3868
DraIII	CACNNNGTG	1 cut(s)	3911
EaeI	YGGCCR	3 cut(s)	1663, 1849, 3668
Eam1104I	CTCTTC	3 cut(s)	1258, 3041, 3913
EarI	CTCTTC	3 cut(s)	1258, 3041, 3913
Eco130I	CCWWGG	7 cut(s)	524, 1337, 1842, 2655, 3491, 4044, 4133
Eco24I	GRGCYC	5 cut(s)	95, 447, 1328, 1446, 2334
Eco31I	GGTCTC	1 cut(s)	1169
Eco32I	GATATC	1 cut(s)	1861
Eco47I	GGWCC	7 cut(s)	521, 1150, 1741, 2920, 3730, 3737, 3772
Eco47III	AGCGCT	1 cut(s)	2267
Eco57I	CTGAAG	3 cut(s)	270, 413, 3093
Eco72I	CACGTG	2 cut(s)	2183, 3041
Eco88I	CYCGRG	3 cut(s)	446, 1146, 3733
EcoO109I	RGGNCCY	8 cut(s)	1150, 1610, 2331, 2920, 3730, 3737, 3772, 4169
EcoRI	GAATTC	2 cut(s)	2159, 3542
EcoRII	CCWGG	9 cut(s)	78, 545, 1114, 1210, 1923, 2203, 2692, 3785, 4178
EcoRV	GATATC	1 cut(s)	1861
EcoT14I	CCWWGG	7 cut(s)	524, 1337, 1842, 2655, 3491, 4044, 4133
EcoT38I	GRGCYC	5 cut(s)	95, 447, 1328, 1446, 2334
ErhI	CCWWGG	7 cut(s)	524, 1337, 1842, 2655, 3491, 4044, 4133
FalI	AAGNNNNNCTT	6 cut(s)	1255, 1287, 2255, 2287, 3280, 3312
FaqI	GGGAC	8 cut(s)	472, 1163, 2046, 3260, 3461, 3716, 3785, 4161
FauI	CCCGC	3 cut(s)	282, 2522, 2630
FbaI	TGATCA	3 cut(s)	1549, 1792, 1882
FokI	GGATG	9 cut(s)	257, 764, 2263, 2551, 2783, 2894, 3509, 4086, 4166
FriOI	GRGCYC	5 cut(s)	95, 447, 1328, 1446, 2334
GlaI	GCGC	4 cut(s)	1016, 1203, 1228, 2267
GsuI	CTGGAG	3 cut(s)	830, 2412, 4053
HaeII	RGCGCY	2 cut(s)	1205, 2269
HaeIII	GGCC	9 cut(s)	378, 1611, 1665, 1851, 2332, 3018, 3670, 3716, 4170
HapII	CCGG	5 cut(s)	749, 1106, 1864, 3103, 3734
HhaI	GCGC	4 cut(s)	1017, 1204, 1229, 2268
Hin6I	GCGC	4 cut(s)	1015, 1202, 1227, 2266
HinP1I	GCGC	4 cut(s)	1015, 1202, 1227, 2266
HincII	GTYRAC	1 cut(s)	588
HindII	GTYRAC	1 cut(s)	588
HindIII	AAGCTT	3 cut(s)	226, 2552, 2762
HpaII	CCGG	5 cut(s)	749, 1106, 1864, 3103, 3734
HphI	GGTGA	9 cut(s)	607, 1451, 1583, 1892, 1957, 2039, 2147, 3820, 3923
Hpy166II	GTNNAC	3 cut(s)	588, 2186, 2287
Hpy8I	GTNNAC	3 cut(s)	588, 2186, 2287
Hpy99I	CGWCG	3 cut(s)	3401, 3938, 3941
HpyCH4IV	ACGT	5 cut(s)	1019, 2035, 2182, 3040, 4029
HpySE526I	ACGT	5 cut(s)	1019, 2035, 2182, 3040, 4029
HspAI	GCGC	4 cut(s)	1015, 1202, 1227, 2266
KflI	GGGWCCC	2 cut(s)	3730, 3772
Kpn2I	TCCGGA	1 cut(s)	748
Ksp22I	TGATCA	3 cut(s)	1549, 1792, 1882
LguI	GCTCTTC	2 cut(s)	3041, 3913
LmnI	GCTCC	8 cut(s)	121, 1381, 1678, 1927, 2116, 2206, 2413, 2567
MaeII	ACGT	5 cut(s)	1019, 2035, 2182, 3040, 4029
MaeIII	GTNAC	8 cut(s)	595, 2296, 2500, 2746, 2973, 3140, 3211, 3499
MbiI	CCGCTC	1 cut(s)	277
MfeI	CAATTG	2 cut(s)	1869, 2628
MflI	RGATCY	5 cut(s)	841, 1345, 1389, 2437, 3307
MhlI	GDGCHC	8 cut(s)	29, 95, 163, 447, 1328, 1446, 2334, 2989
MlsI	TGGCCA	2 cut(s)	1665, 1851
MluNI	TGGCCA	2 cut(s)	1665, 1851
MlyI	GAGTC	4 cut(s)	377, 592, 3812, 3842
MmeI	TCCRAC	7 cut(s)	767, 914, 948, 2415, 2446, 3105, 3544
Mox20I	TGGCCA	2 cut(s)	1665, 1851
MroI	TCCGGA	1 cut(s)	748
MroXI	GAANNNNTTC	2 cut(s)	255, 3529
MscI	TGGCCA	2 cut(s)	1665, 1851
MslI	CAYNNNNRTG	4 cut(s)	768, 1586, 3222, 3833
Msp20I	TGGCCA	2 cut(s)	1665, 1851
MspA1I	CMGCKG	5 cut(s)	248, 299, 1292, 2529, 3274
MspCI	CTTAAG	2 cut(s)	2228, 2765
MspI	CCGG	5 cut(s)	749, 1106, 1864, 3103, 3734
MunI	CAATTG	2 cut(s)	1869, 2628
Mva1269I	GAATGC	3 cut(s)	1785, 3824, 4204
MvaI	CCWGG	9 cut(s)	80, 547, 1116, 1212, 1925, 2205, 2694, 3787, 4180
NciI	CCSGG	3 cut(s)	1865, 3734, 3735
NcoI	CCATGG	2 cut(s)	2655, 3491
NheI	GCTAGC	1 cut(s)	1403
NmuCI	GTSAC	2 cut(s)	595, 2500
NspI	RCATGY	3 cut(s)	582, 1128, 2403
OliI	CACNNNNGTG	1 cut(s)	3833
PagI	TCATGA	3 cut(s)	685, 1789, 2953
PaqCI	CACCTGC	1 cut(s)	1793
PciI	ACATGT	1 cut(s)	1124
PciSI	GCTCTTC	2 cut(s)	3041, 3913
PcsI	WCGNNNNNNNCGW	3 cut(s)	320, 329, 1741
PctI	GAATGC	3 cut(s)	1785, 3824, 4204
PdmI	GAANNNNTTC	2 cut(s)	255, 3529
PflMI	CCANNNNNTGG	1 cut(s)	4149
PfoI	TCCNGGA	1 cut(s)	2203
PinAI	ACCGGT	1 cut(s)	1105
PleI	GAGTC	4 cut(s)	377, 592, 3811, 3842
PmaCI	CACGTG	2 cut(s)	2183, 3041
PmlI	CACGTG	2 cut(s)	2183, 3041
PpsI	GAGTC	4 cut(s)	377, 592, 3811, 3842
Ppu21I	YACGTR	2 cut(s)	2183, 3041
PpuMI	RGGWCCY	5 cut(s)	1150, 2920, 3730, 3737, 3772
PscI	ACATGT	1 cut(s)	1124
PshBI	ATTAAT	1 cut(s)	663
Psp5II	RGGWCCY	5 cut(s)	1150, 2920, 3730, 3737, 3772
Psp6I	CCWGG	9 cut(s)	78, 545, 1114, 1210, 1923, 2203, 2692, 3785, 4178
PspCI	CACGTG	2 cut(s)	2183, 3041
PspGI	CCWGG	9 cut(s)	78, 545, 1114, 1210, 1923, 2203, 2692, 3785, 4178
PspOMI	GGGCCC	1 cut(s)	2330
PspPPI	RGGWCCY	5 cut(s)	1150, 2920, 3730, 3737, 3772
PstI	CTGCAG	3 cut(s)	247, 1389, 2107
PsuI	RGATCY	5 cut(s)	841, 1345, 1389, 2437, 3307
PvuII	CAGCTG	4 cut(s)	248, 299, 1292, 3274
RsaI	GTAC	7 cut(s)	502, 623, 851, 1698, 2130, 3232, 3886
RsaNI	GTAC	7 cut(s)	501, 622, 850, 1697, 2129, 3231, 3885
RseI	CAYNNNNRTG	4 cut(s)	768, 1586, 3222, 3833
SapI	GCTCTTC	2 cut(s)	3041, 3913
ScaI	AGTACT	3 cut(s)	851, 2130, 3232
SchI	GAGTC	4 cut(s)	377, 592, 3812, 3842
SduI	GDGCHC	8 cut(s)	29, 95, 163, 447, 1328, 1446, 2334, 2989
SfcI	CTRYAG	6 cut(s)	243, 1197, 1287, 1385, 2103, 2589
SinI	GGWCC	7 cut(s)	521, 1150, 1741, 2920, 3730, 3737, 3772
SmaI	CCCGGG	1 cut(s)	3735
SmiMI	CAYNNNNRTG	4 cut(s)	768, 1586, 3222, 3833
SmlI	CTYRAG	7 cut(s)	162, 308, 1066, 2120, 2228, 2765, 3741
SmoI	CTYRAG	7 cut(s)	162, 308, 1066, 2120, 2228, 2765, 3741
SpeI	ACTAGT	1 cut(s)	3263
SsiI	CCGC	9 cut(s)	268, 275, 389, 1676, 1960, 2529, 2637, 2812, 3667
StyI	CCWWGG	7 cut(s)	524, 1337, 1842, 2655, 3491, 4044, 4133
TaiI	ACGT	5 cut(s)	1022, 2038, 2185, 3043, 4032
TatI	WGTACW	6 cut(s)	500, 849, 1696, 2128, 3230, 3884
TauI	GCSGC	3 cut(s)	391, 1962, 3670
TscAI	CASTG	5 cut(s)	368, 684, 934, 2220, 3838
TseFI	GTSAC	2 cut(s)	595, 2500
Tsp45I	GTSAC	2 cut(s)	595, 2500
TspDTI	ATGAA	9 cut(s)	248, 674, 717, 768, 1778, 3336, 3396, 3555, 3781
TspGWI	ACGGA	4 cut(s)	1338, 1733, 3385, 3536
TspMI	CCCGGG	1 cut(s)	3733
TspRI	CASTG	5 cut(s)	368, 684, 934, 2220, 3838
Van91I	CCANNNNNTGG	1 cut(s)	4149
Vha464I	CTTAAG	2 cut(s)	2228, 2765
VpaK11BI	GGWCC	7 cut(s)	521, 1150, 1741, 2920, 3730, 3737, 3772
VspI	ATTAAT	1 cut(s)	663
XapI	RAATTY	6 cut(s)	451, 1369, 1433, 2159, 2340, 3542
XbaI	TCTAGA	1 cut(s)	3310
XceI	RCATGY	3 cut(s)	582, 1128, 2403
XmaI	CCCGGG	1 cut(s)	3733
XmaJI	CCTAGG	1 cut(s)	524
XmnI	GAANNNNTTC	2 cut(s)	255, 3529
ZrmI	AGTACT	3 cut(s)	851, 2130, 3232

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.