Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0005762

Physical Location & Seq

Genomic Coordinates Forward (+)

27713963 .. 27719642

Gene Structure 5680 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1203100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4239 bp

ATGGCCGGTGTTCGGGAAACAGCCGCCGCCGAGTTCTTACAAGGCACGAATAGGCAGAGCCTTTTTTTGCAGAGGAAGTCGGTTAAAGGAAGAAGCCAGGTGCTTTGGGGCTCGCTTCACGGGCGAAGTTCAGCTCCCAGTTTTGGTAATAGGAGAGGTGTTTCTTTGAGGTGTCGTGCTCAAGAAAAGCCCAGAGCTGTGGTTTCAGGTGGTGTGAGCAGCTTGGTAGATGATGAGCAGTCAAGCTTGGTTGAGAAGCCTGCAGCTGAAGTTACTCATTTTTTTCGCATCCCGCTCATTCAGGAGAGTGCAACAGCTGAGCTTCTCAAGACGGTTCAGACAAAGATTACGAATCAGACTGTTGGTTTGAAAACTGAGCAGTGCTTCAACATTGGCCTTGACTCGGCGCTTTCTAGCGACAAGGTTTTGGTTTTGAAGTGGCTTCTTCAGGAGACTTATGAGCCCGAGAATTTGGGGACTGAGAGCTTTCTTGAAAAGAAAAAGCAGGAAGGGCTTAGTACAGTTATTATTGAGGTTGGTCCTAGGTTGTCATTTACAACAGCCTGGTCTTCTAATGCTGTATCGATTTGTCGAGCATGTGGGTTGACAGAGGTGACTCGTTTGGAACGGTCAAGGAGGTACTTGCTGTTTAGTAAGGGATCACTTCATGATCATCAGATTAATGAGTTTGCTGCACTGGTTCATGATCGAATGACTGAGTGTGTGTATGCCCAGAAGCTAGTTTCTTTTGAAACGAGTGTAGTTCCGGATGAAGTTCGTCATGTACATGTCATGGAGAGGGGTCGGAAGGCATTGGAGGAGATAAATCAAGAAATGGGTTTGGCATTTGATGAGCAGGATCTCCAGTACTACACTAGGCTATTCAAGGACGAAATCCAAAGGAATCCAACAACAGTTGAGCTGTTTGATATTGCACAATCCAACAGTGAGCATAGTAGGCACTGGTTTTTTACTGGCAAAATGATTATAGATGGACAGCCTATGGATAGAACTCTGATGCAGATTGTGAAGAGCACGTTGCGGGCAAACCCAAACAATTCTGTAATTGGCTTCAAGGATAACTCAAGTGCAATCAAGGGGTTTCTTGTGAAGCAAATGCGACCGGTTCAGCCAGGTTCAACATGTCCATTGAGCATAGCTACTCGGGACCTTGATATTTTATTTACTGCTGAGACCCATAATTTCCCATGTGCTGTAGCGCCTTACCCTGGTGCAGAGACAGGCGCCGGAGGTCGAATCAGGGATACCCATGCGACTGGAAGAGGGTCTTTTGTTGTAGCATCTACAGCTGGTTATTGTGTTGGGAATCTCAATATGGAGGGCTCTTATGCACCTTGGGAAGATCCGTCTTTTGCTTATCCATCAAATTTGGCTCCACCTCTGCAGATCCTTATAGATGCTAGCAATGGTGCATCAGACTATGGGAACAAATTTGGTGAGCCCTTGATTCAGGGCTACACTAGAACATTTGGAATGAGACTTCCAAGTGGAGACAGGCGAGAATGGTTGAAGCCAATCATGTTTAGTGGAGGCATTGGGCAGATTGATCACACCCATATAACAAAAGGTGAGCCTGACATTGGTATGCTGGTTGTTAAAGTTGGAGGCCCTGCTTATCGTATTGGAATGGGGGGTGGGGCAGCATCGAGCATGGTTAGTGGCCAGAATGATGCGGAGCTTGATTTTAATGCTGTACAGCGTGGAGATGCAGAGATGGCACAGAAACTTTATCGTGTGGTCCGTGCCTGTATTGAGATGGGGGAGAATAACCCTATCATTAGCATTCATGATCAGGGGGCAGGTGGAAACTGCAATGTTGTTAAGGAAATTATATACCCCAAGGGCGGCCAGATTGATATCCGGGCAATTGTAGTTGGTGATCACACAATGTCTGTCTTGGAGATATGGGGTGCAGAATACCAGGAGCAAGATGCAATCTTGGTGAAGCCTGAAAGCCGCCACCTCTTGCAATCAATATGTGAAAGAGAAAGGGTTTCTATGGCTGTTATTGGAACAATAAATGGTGAGGGACGTGCTGTTTTAATTGATAGCTTAGCTATTAAAAAATGTGAATCTAGTGGAATCCCTCCCCCTCCTCCTGCTGTCGATTTAGAGCTTGAAAAAGTACTTGGTGATATGCCACAGAAAAGTTTTGAATTCCACAGGACGACTGATGCACGAGAACCACTTGATATTGCTCCTGGAATAACAGTGATGGATTCACTTAAGAGGGTGTTGAGACTTCCATCTGTTTGTTCAAAGCGCTTCTTGACATCCAAAGTAGACAGGTGTGTTACAGGTCTTGTAGCACAGCAGCAAACTGTTGGGCCCTTGCAAATTCCTCTATCTGATGTTGCAGTAATAGCGCAGACTTTTACTGACCTGACTGGAGGTGCATGTGCCATTGGGGAACAGCCAATCAAAGGTTTGTTGGATCCAAAAGCAATGGCGAGATTGGCTGTTGGAGAAGCACTCACAAATCTTGTTTGGGCAAAGGTCACTTCTCTATCTGATGTTAAAGCGAGCGGGAATTGGATGTATGCAGCCAAGCTTAATGGGGAAGGAGCAGCTATGTATGATGCTGCTACAGCTCTTTCAGAGGCAATGATCGAACTTGGTATTGCAATTGATGGTGGGAAGGACAGTCTTTCCATGGCAGCCCATGTTGCAGGCGAGGTTGTTAAGGCACCTGGCAATCTTGTAATGAGTGTTTATTGCACTTGTCCTGACATAACAAAAACAGTAACCCCAGATTTAAAGCTCAAGGATGATGGCGTGCTGCTTCACATTGATTTGGCAAAGGGAAAGCGGAGGTTAGGTGGGTCTGCTCTTGCTCAGGTTTTTGACCAAATTGGCAATGATTGCCCTGACATTGAGGACGTTCCTTATCTAAAACGAGTTTTTGAGGGAGTTCAGGACCTTCTTTCTGACGAATTGATCTCTGCTGGTCATGATATTAGTGATGGTGGGCTACTGGTCTGTGCCCTTGAAATGGCATTTTCTGGGAATTGTGGCATTACTTTGGACTTGACTTCACATGGGAAGAGCCTATTCCAAATTCTTTTTGCTGAAGAACTTGGTCTGGTTATTGAGGTAAGCCGGAATAACTTAGATTTGGTGTTGGAAAAACTGAGCAGTAACAGTATTTTGGCTGAGATTATTGGTCAAGTTAGTGCCACACCCTCAGTAGAGTTAAAGGTTGATGGGGTTACACATTTGAATGAGAGTACTTCTTTTCTAAGGGACTTGTGGGAGGACACTAGTTTTCAGCTGGAAAGACTACAAAGGTTGGCTTCTTGTGTAGATCTAGAAAAAGAAGGGTTGAAAGATAGGCATGAGCCTTCATGGGATTTGTCCTTCACTCCGTCTTTCACAGATGAAAAGTATATGACCGTCGCTTGCAAACCAAAAGTGGCTATAATTCGAGAAGAAGGCAGCAATGGGGACAGAGAAATGTCGGCAGCATTTTATGCTTCTGGATTTGAACCTTGGGATGTTACAATGTCAGACCTTCTTAATGGAACCATTTCATTGCAAGAATTCCGTGGAATTGCGTTTGTTGGAGGTTTTAGCTACGCTGATGTGCTTGATTCTGCAAAAGGTTGGTCTGCATCAATTCGATTTAATCAGCCCCTTCTAAATCAATTTCAAGAGTTTTACAAGCGGCCAGACACTTTTAGTCTTGGAGTTTGCAACGGGTGTCAGCTTATGGCCCTTCTGGGGTGGGTCCCGGGTCCTCAAGTTGGTGGTGTGCTAGGTGGTGGTGGGGACCCTTCGCAGCCCAGGTTCATTCACAACGAGTCTGGACGGTTTGAATGCCGATTCACCAGTGTGGCAATAAAGGACTCGCCAGCTATAATGTTTAAAGGAATGGAGGGCAGTACATTGGGTGTGTGGGCTGCACATGGTGAGGGAAGAGCATATTTCCCCGACGACGGTGTTCTTGATCGTTTGCTTCACTCAAAGTTGGCACCAGTAAGGTATTGCGATGATGATGGTAATGAGACAGAAGTTTACCCTTTCAACGTCAATGGCTCTCCCTTGGGGGTTGCAGCAATTTGTTCTCCAGATGGGAGGCATCTAGCCATGATGCCTCATCCAGAGCGTTGCTTCTTGATGTGGCAATTCCCCTGGTATCCCAAGCAATGGGATGTGGAAAAGAAAGGCCCTAGTCCCTGGTTGCGAATGTTCCAGAATGCTAGAGAGTGGTGTTGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0003674 GO:0003824 GO:0004642 GO:0005488 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0007275 GO:0008144 GO:0008150 GO:0009507 GO:0009532 GO:0009536 GO:0009555 GO:0009570 GO:0016874 GO:0016879 GO:0016884 GO:0017076 GO:0030554 GO:0032501 GO:0032502 GO:0032553 GO:0032555 GO:0032559 GO:0035639 GO:0036094 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0048229 GO:0048856 GO:0055046 GO:0097159 GO:0097367 GO:1901265 GO:1901363

KEGG Pathways

Metabolic & Signaling

ko00230 ko01100 ko01110 ko01130 map00230 map01100 map01110 map01130

Pfam Domains

Protein Families

AIRS_C GATase_5

Protein Analysis

1413

Amino Acids

154.39

Weight (kDa)

5.4

Isoelectric Point (pI)

37.93

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FGAR-AT_N	PF18076	128 - 245	8.1e-21	Formylglycinamide ribonucleotide amidotransferase N-terminal
FGAR-AT_linker	PF18072	272 - 321	9e-11	Formylglycinamide ribonucleotide amidotransferase linker domain
AIRS_C	PF02769	535 - 689	3.3e-26	AIR synthase related protein, C-terminal domain
FGAR-AT_PurM_N-like	PF22689	760 - 913	1.8e-52	FGAR-AT PurM_N-like domain
AIRS_C	PF02769	944 - 1074	2.1e-18	AIR synthase related protein, C-terminal domain
GATase_5	PF13507	1142 - 1411	9.3e-106	CobB/CobQ-like glutamine amidotransferase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0005762)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G74260
fragaria_vesca	FvH4_5g19530 FvH4_5g19530 FvH4_5g19530
malus_domestica	MD03G1203100.v1.1 MD04G1022300.v1.1
prunus_persica	Prupe.1G542100_v2.0.a1 Prupe.1G542100_v2.0.a1 Prupe.1G542100_v2.0.a1 Prupe.1G542100_v2.0.a1 Prupe.1G542100_v2.0.a1 Prupe.8G024000_v2.0.a1 Prupe.8G024000_v2.0.a1 Prupe.8G024000_v2.0.a1
pyrus_communis	pycom04g01890 pycom04g01930
rosa_chinensis	RchiOBHm_Chr7g0204141
rosa_laevigata	RLG00000003485
rosa_multiflora	Rmu_sc0001311.1_g000031
rosa_roxburghii	Rroxscaffold_3G00253310
rosa_rugosa	Rorug07G0081100 Rorug07G0081200 Rorug07G0081200
rosa_samantha	Rh7AG211000 Rh7BG209600 Rh7CG223500 Rh7DG217800
rosa_wichuraiana	Rw7G018360

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1811
Acc36I	ACCTGC	1 cut(s)	1811
AccB1I	GGYRCC	3 cut(s)	1244, 2707, 3991
AccB7I	CCANNNNNTGG	1 cut(s)	4167
AccBSI	CCGCTC	2 cut(s)	295, 2547
AccI	GTMKAC	1 cut(s)	2304
AccIII	TCCGGA	1 cut(s)	766
AclWI	GGATC	6 cut(s)	667, 867, 1358, 1402, 2450, 2463
AcoI	YGGCCR	4 cut(s)	3, 1681, 1867, 3686
AcsI	RAATTY	7 cut(s)	469, 1387, 1451, 2177, 2358, 3078, 3560
AcuI	CTGAAG	3 cut(s)	288, 431, 3111
AcyI	GRCGYC	1 cut(s)	1245
AdeI	CACNNNGTG	1 cut(s)	3929
AfaI	GTAC	8 cut(s)	520, 641, 786, 869, 1716, 2148, 3250, 3904
AfeI	AGCGCT	1 cut(s)	2285
AflII	CTTAAG	1 cut(s)	2246
AflIII	ACRYGT	2 cut(s)	787, 1142
AgeI	ACCGGT	1 cut(s)	1123
AhlI	ACTAGT	1 cut(s)	3281
AjiI	CACGTC	1 cut(s)	2054
AjuI	GAANNNNNNNTTGG	2 cut(s)	2133, 2165
AleI	CACNNNNGTG	1 cut(s)	3851
Alw21I	GWGCWC	2 cut(s)	181, 1037
Alw26I	GTCTC	7 cut(s)	446, 1187, 1232, 1492, 1506, 2254, 4019
AlwI	GGATC	6 cut(s)	667, 867, 1358, 1402, 2450, 2463
Ama87I	CYCGRG	3 cut(s)	464, 1164, 3751
Aor13HI	TCCGGA	1 cut(s)	766
Aor51HI	AGCGCT	1 cut(s)	2285
AoxI	GGCC	9 cut(s)	3, 394, 1627, 1681, 1867, 2348, 3686, 3732, 4186
ApaI	GGGCCC	1 cut(s)	2352
ApoI	RAATTY	7 cut(s)	469, 1387, 1451, 2177, 2358, 3078, 3560
ArsI	GACNNNNNNTTYG	4 cut(s)	349, 381, 3611, 3643
AseI	ATTAAT	1 cut(s)	681
AsiGI	ACCGGT	1 cut(s)	1123
Asp700I	GAANNNNTTC	1 cut(s)	3547
AspA2I	CCTAGG	1 cut(s)	542
AspLEI	GCGC	5 cut(s)	409, 1222, 1247, 2286, 2389
AsuC2I	CCSGG	3 cut(s)	1883, 3752, 3753
AsuHPI	GGTGA	9 cut(s)	625, 1469, 1601, 1910, 1975, 2057, 2165, 3838, 3941
AsuNHI	GCTAGC	1 cut(s)	1421
AvaI	CYCGRG	3 cut(s)	464, 1164, 3751
AvaII	GGWCC	7 cut(s)	539, 1168, 1759, 2938, 3748, 3755, 3790
AvrII	CCTAGG	1 cut(s)	542
BaeGI	GKGCMC	2 cut(s)	2352, 3007
BalI	TGGCCA	1 cut(s)	1683
BamHI	GGATCC	1 cut(s)	2455
BanI	GGYRCC	3 cut(s)	1244, 2707, 3991
BanII	GRGCYC	5 cut(s)	113, 465, 1346, 1464, 2352
BauI	CACGAG	1 cut(s)	2199
BbsI	GAAGAC	1 cut(s)	561
Bbv12I	GWGCWC	2 cut(s)	181, 1037
BciVI	GTATCC	2 cut(s)	1258, 4167
BclI	TGATCA	4 cut(s)	670, 1567, 1810, 1900
BcnI	CCSGG	3 cut(s)	1883, 3752, 3753
BcoDI	GTCTC	7 cut(s)	446, 1187, 1232, 1492, 1506, 2254, 4019
BcuI	ACTAGT	1 cut(s)	3281
BfmI	CTRYAG	5 cut(s)	261, 1215, 1305, 1403, 2607
BfoI	RGCGCY	4 cut(s)	410, 1223, 1248, 2287
BfrI	CTTAAG	1 cut(s)	2246
BfuAI	ACCTGC	1 cut(s)	1811
BfuI	GTATCC	2 cut(s)	1258, 4167
BglII	AGATCT	1 cut(s)	3325
BlnI	CCTAGG	1 cut(s)	542
BlpI	GCTNAGC	2 cut(s)	318, 2074
BmcAI	AGTACT	3 cut(s)	869, 2148, 3250
Bme18I	GGWCC	7 cut(s)	539, 1168, 1759, 2938, 3748, 3755, 3790
BmeT110I	CYCGRG	3 cut(s)	464, 1164, 3751
BmgBI	CACGTC	1 cut(s)	2054
BmrI	ACTGGG	1 cut(s)	132
BmtI	GCTAGC	1 cut(s)	1425
BmuI	ACTGGG	1 cut(s)	132
BpiI	GAAGAC	1 cut(s)	561
BplI	GAGNNNNNCTC	2 cut(s)	2478, 2510
BpmI	CTGGAG	3 cut(s)	848, 2430, 4071
Bpu10I	CCTNAGC	1 cut(s)	2856
Bpu1102I	GCTNAGC	2 cut(s)	318, 2074
BpuEI	CTTGAG	5 cut(s)	165, 311, 1069, 2768, 3744
BpuMI	CCSGG	3 cut(s)	1883, 3752, 3753
Bsa29I	ATCGAT	1 cut(s)	584
BsaBI	GATNNNNATC	1 cut(s)	1877
BsaHI	GRCGYC	1 cut(s)	1245
BsaI	GGTCTC	1 cut(s)	1187
BsaWI	WCCGGW	2 cut(s)	766, 1123
BsaXI	ACNNNNNCTCC	2 cut(s)	2824, 2854
Bse118I	RCCGGY	2 cut(s)	5, 1123
Bse3DI	GCAATG	8 cut(s)	1432, 1840, 2472, 2631, 2884, 3466, 3551, 4172
Bse8I	GATNNNNATC	1 cut(s)	1877
BseAI	TCCGGA	1 cut(s)	766
BseCI	ATCGAT	1 cut(s)	584
BseGI	GGATG	8 cut(s)	288, 775, 2294, 2562, 2794, 3520, 4117, 4177
BseJI	GATNNNNATC	1 cut(s)	1877
BseMI	GCAATG	8 cut(s)	1432, 1840, 2472, 2631, 2884, 3466, 3551, 4172
BseMII	CTCAG	9 cut(s)	309, 366, 471, 708, 1182, 2870, 3143, 3165, 3219
BseRI	GAGGAG	2 cut(s)	833, 2106
BseSI	GKGCMC	2 cut(s)	2352, 3007
BsgI	GTGCAG	4 cut(s)	678, 1254, 1953, 3906
Bsh1285I	CGRYCG	1 cut(s)	1124
BshFI	GGCC	9 cut(s)	5, 396, 1629, 1683, 1869, 2350, 3688, 3734, 4188
BshNI	GGYRCC	3 cut(s)	1244, 2707, 3991
BshTI	ACCGGT	1 cut(s)	1123
BshVI	ATCGAT	1 cut(s)	584
BsiEI	CGRYCG	1 cut(s)	1124
BsiHKAI	GWGCWC	2 cut(s)	181, 1037
BsiHKCI	CYCGRG	3 cut(s)	464, 1164, 3751
BsiSI	CCGG	7 cut(s)	6, 767, 1124, 1248, 1882, 3121, 3752
BslFI	GGGAC	8 cut(s)	490, 1181, 2064, 3278, 3479, 3734, 3803, 4179
BsmAI	GTCTC	7 cut(s)	446, 1187, 1232, 1492, 1506, 2254, 4019
BsmFI	GGGAC	8 cut(s)	490, 1181, 2064, 3278, 3479, 3734, 3803, 4179
BsmI	GAATGC	3 cut(s)	1803, 3842, 4222
BsnI	GGCC	9 cut(s)	5, 396, 1629, 1683, 1869, 2350, 3688, 3734, 4188
Bso31I	GGTCTC	1 cut(s)	1187
BsoBI	CYCGRG	3 cut(s)	464, 1164, 3751
Bsp120I	GGGCCC	1 cut(s)	2348
Bsp1286I	GDGCHC	8 cut(s)	113, 181, 465, 1037, 1346, 1464, 2352, 3007
Bsp13I	TCCGGA	1 cut(s)	766
Bsp1407I	TGTACA	2 cut(s)	784, 1714
Bsp1720I	GCTNAGC	2 cut(s)	318, 2074
Bsp19I	CCATGG	1 cut(s)	2673
BspANI	GGCC	9 cut(s)	5, 396, 1629, 1683, 1869, 2350, 3688, 3734, 4188
BspCNI	CTCAG	9 cut(s)	310, 367, 472, 709, 1183, 2869, 3144, 3166, 3218
BspDI	ATCGAT	1 cut(s)	584
BspEI	TCCGGA	1 cut(s)	766
BspHI	TCATGA	4 cut(s)	667, 703, 1807, 2971
BspMAI	CTGCAG	2 cut(s)	265, 1407
BspMI	ACCTGC	1 cut(s)	1811
BspOI	GCTAGC	1 cut(s)	1425
BspPI	GGATC	6 cut(s)	667, 867, 1358, 1402, 2450, 2463
BspQI	GCTCTTC	3 cut(s)	1025, 3059, 3931
BspT107I	GGYRCC	3 cut(s)	1244, 2707, 3991
BspTI	CTTAAG	1 cut(s)	2246
BspTNI	GGTCTC	1 cut(s)	1187
BsrBI	CCGCTC	2 cut(s)	295, 2547
BsrDI	GCAATG	8 cut(s)	1432, 1840, 2472, 2631, 2884, 3466, 3551, 4172
BsrFI	RCCGGY	2 cut(s)	5, 1123
BsrGI	TGTACA	2 cut(s)	784, 1714
BssAI	RCCGGY	2 cut(s)	5, 1123
BssNI	GRCGYC	1 cut(s)	1245
BssSI	CACGAG	1 cut(s)	2199
BssT1I	CCWWGG	6 cut(s)	542, 1355, 1860, 2673, 3509, 4062
Bst2BI	CACGAG	1 cut(s)	2199
Bst6I	CTCTTC	4 cut(s)	1025, 1276, 3059, 3931
BstACI	GRCGYC	1 cut(s)	1245
BstAFI	CTTAAG	1 cut(s)	2246
BstAPI	GCANNNNNTGC	2 cut(s)	2883, 3491
BstAUI	TGTACA	2 cut(s)	784, 1714
BstC8I	GCNNGC	9 cut(s)	113, 261, 1044, 1423, 2545, 2692, 2798, 3421, 3873
BstDSI	CCRYGG	2 cut(s)	2673, 3565
BstF5I	GGATG	8 cut(s)	288, 775, 2294, 2562, 2794, 3520, 4117, 4177
BstH2I	RGCGCY	4 cut(s)	410, 1223, 1248, 2287
BstHHI	GCGC	5 cut(s)	409, 1222, 1247, 2286, 2389
BstMAI	GTCTC	7 cut(s)	446, 1187, 1232, 1492, 1506, 2254, 4019
BstMCI	CGRYCG	1 cut(s)	1124
BstNSI	RCATGY	4 cut(s)	600, 791, 1146, 2421
BstSFI	CTRYAG	5 cut(s)	261, 1215, 1305, 1403, 2607
BstSLI	GKGCMC	2 cut(s)	2352, 3007
BstV2I	GAAGAC	1 cut(s)	561
BstX2I	RGATCY	5 cut(s)	859, 1363, 1407, 2455, 3325
BstXI	CCANNNNNNTGG	2 cut(s)	199, 1277
BstYI	RGATCY	5 cut(s)	859, 1363, 1407, 2455, 3325
Bsu15I	ATCGAT	1 cut(s)	584
BsuI	GTATCC	2 cut(s)	1258, 4167
BsuRI	GGCC	9 cut(s)	5, 396, 1629, 1683, 1869, 2350, 3688, 3734, 4188
BsuTUI	ATCGAT	1 cut(s)	584
BtgI	CCRYGG	2 cut(s)	2673, 3565
BtgZI	GCGATG	1 cut(s)	4023
BtrI	CACGTC	1 cut(s)	2054
BtsCI	GGATG	8 cut(s)	288, 775, 2294, 2562, 2794, 3520, 4117, 4177
BtsI	GCAGTG	1 cut(s)	386
BtsIMutI	CAGTG	6 cut(s)	386, 695, 952, 961, 2238, 3856
BveI	ACCTGC	1 cut(s)	1811
Cac8I	GCNNGC	9 cut(s)	113, 261, 1044, 1423, 2545, 2692, 2798, 3421, 3873
CciI	TCATGA	4 cut(s)	667, 703, 1807, 2971
CfoI	GCGC	5 cut(s)	409, 1222, 1247, 2286, 2389
Cfr10I	RCCGGY	2 cut(s)	5, 1123
Cfr9I	CCCGGG	1 cut(s)	3751
ClaI	ATCGAT	1 cut(s)	584
Csp6I	GTAC	8 cut(s)	519, 640, 785, 868, 1715, 2147, 3249, 3903
CspAI	ACCGGT	1 cut(s)	1123
CviQI	GTAC	8 cut(s)	519, 640, 785, 868, 1715, 2147, 3249, 3903
DinI	GGCGCC	1 cut(s)	1246
DraI	TTTAAA	2 cut(s)	2778, 3886
DraIII	CACNNNGTG	1 cut(s)	3929
EaeI	YGGCCR	4 cut(s)	3, 1681, 1867, 3686
Eam1104I	CTCTTC	4 cut(s)	1025, 1276, 3059, 3931
EarI	CTCTTC	4 cut(s)	1025, 1276, 3059, 3931
Eco130I	CCWWGG	6 cut(s)	542, 1355, 1860, 2673, 3509, 4062
Eco24I	GRGCYC	5 cut(s)	113, 465, 1346, 1464, 2352
Eco31I	GGTCTC	1 cut(s)	1187
Eco32I	GATATC	1 cut(s)	1879
Eco47I	GGWCC	7 cut(s)	539, 1168, 1759, 2938, 3748, 3755, 3790
Eco47III	AGCGCT	1 cut(s)	2285
Eco57I	CTGAAG	3 cut(s)	288, 431, 3111
Eco88I	CYCGRG	3 cut(s)	464, 1164, 3751
EcoO109I	RGGNCCY	8 cut(s)	1168, 1628, 2349, 2938, 3748, 3755, 3790, 4187
EcoRI	GAATTC	2 cut(s)	2177, 3560
EcoRV	GATATC	1 cut(s)	1879
EcoT14I	CCWWGG	6 cut(s)	542, 1355, 1860, 2673, 3509, 4062
EcoT38I	GRGCYC	5 cut(s)	113, 465, 1346, 1464, 2352
EgeI	GGCGCC	1 cut(s)	1246
EheI	GGCGCC	1 cut(s)	1246
ErhI	CCWWGG	6 cut(s)	542, 1355, 1860, 2673, 3509, 4062
FalI	AAGNNNNNCTT	6 cut(s)	1273, 1305, 2273, 2305, 3298, 3330
FaqI	GGGAC	8 cut(s)	490, 1181, 2064, 3278, 3479, 3734, 3803, 4179
FauI	CCCGC	3 cut(s)	300, 1035, 2540
FbaI	TGATCA	4 cut(s)	670, 1567, 1810, 1900
FblI	GTMKAC	1 cut(s)	2304
FokI	GGATG	8 cut(s)	275, 782, 2281, 2569, 2801, 3527, 4104, 4184
FriOI	GRGCYC	5 cut(s)	113, 465, 1346, 1464, 2352
GlaI	GCGC	5 cut(s)	408, 1221, 1246, 2285, 2388
GsuI	CTGGAG	3 cut(s)	848, 2430, 4071
HaeII	RGCGCY	4 cut(s)	410, 1223, 1248, 2287
HaeIII	GGCC	9 cut(s)	5, 396, 1629, 1683, 1869, 2350, 3688, 3734, 4188
HapII	CCGG	7 cut(s)	6, 767, 1124, 1248, 1882, 3121, 3752
HhaI	GCGC	5 cut(s)	409, 1222, 1247, 2286, 2389
Hin1I	GRCGYC	1 cut(s)	1245
Hin6I	GCGC	5 cut(s)	407, 1220, 1245, 2284, 2387
HinP1I	GCGC	5 cut(s)	407, 1220, 1245, 2284, 2387
HincII	GTYRAC	1 cut(s)	606
HindII	GTYRAC	1 cut(s)	606
HindIII	AAGCTT	2 cut(s)	244, 2570
HpaII	CCGG	7 cut(s)	6, 767, 1124, 1248, 1882, 3121, 3752
HphI	GGTGA	9 cut(s)	625, 1469, 1601, 1910, 1975, 2057, 2165, 3838, 3941
Hpy166II	GTNNAC	3 cut(s)	606, 2305, 4036
Hpy8I	GTNNAC	3 cut(s)	606, 2305, 4036
Hpy99I	CGWCG	3 cut(s)	3419, 3956, 3959
HpyCH4IV	ACGT	4 cut(s)	1037, 2053, 2901, 4047
HpySE526I	ACGT	4 cut(s)	1037, 2053, 2901, 4047
Hsp92I	GRCGYC	1 cut(s)	1245
HspAI	GCGC	5 cut(s)	407, 1220, 1245, 2284, 2387
KasI	GGCGCC	1 cut(s)	1244
KflI	GGGWCCC	2 cut(s)	3748, 3790
Kpn2I	TCCGGA	1 cut(s)	766
Ksp22I	TGATCA	4 cut(s)	670, 1567, 1810, 1900
LguI	GCTCTTC	3 cut(s)	1025, 3059, 3931
LmnI	GCTCC	6 cut(s)	139, 1399, 1696, 1945, 2224, 2585
MaeII	ACGT	4 cut(s)	1037, 2053, 2901, 4047
MaeIII	GTNAC	8 cut(s)	271, 613, 2314, 2518, 2764, 3158, 3229, 3517
MbiI	CCGCTC	2 cut(s)	295, 2547
MfeI	CAATTG	2 cut(s)	1887, 2646
MflI	RGATCY	5 cut(s)	859, 1363, 1407, 2455, 3325
MhlI	GDGCHC	8 cut(s)	113, 181, 465, 1037, 1346, 1464, 2352, 3007
MlsI	TGGCCA	1 cut(s)	1683
MluNI	TGGCCA	1 cut(s)	1683
Mly113I	GGCGCC	1 cut(s)	1245
MlyI	GAGTC	4 cut(s)	395, 610, 3830, 3860
MmeI	TCCRAC	8 cut(s)	785, 932, 966, 1603, 2433, 2464, 3123, 3562
Mox20I	TGGCCA	1 cut(s)	1683
MroI	TCCGGA	1 cut(s)	766
MroXI	GAANNNNTTC	1 cut(s)	3547
MscI	TGGCCA	1 cut(s)	1683
MslI	CAYNNNNRTG	4 cut(s)	786, 1604, 3240, 3851
Msp20I	TGGCCA	1 cut(s)	1683
MspA1I	CMGCKG	4 cut(s)	266, 317, 1310, 3292
MspCI	CTTAAG	1 cut(s)	2246
MspI	CCGG	7 cut(s)	6, 767, 1124, 1248, 1882, 3121, 3752
MunI	CAATTG	2 cut(s)	1887, 2646
Mva1269I	GAATGC	3 cut(s)	1803, 3842, 4222
NarI	GGCGCC	1 cut(s)	1245
NciI	CCSGG	3 cut(s)	1883, 3752, 3753
NcoI	CCATGG	1 cut(s)	2673
NheI	GCTAGC	1 cut(s)	1421
NmeAIII	GCCGAG	2 cut(s)	55, 383
NmuCI	GTSAC	2 cut(s)	613, 2518
NspI	RCATGY	4 cut(s)	600, 791, 1146, 2421
OliI	CACNNNNGTG	1 cut(s)	3851
PagI	TCATGA	4 cut(s)	667, 703, 1807, 2971
PaqCI	CACCTGC	1 cut(s)	1811
PciI	ACATGT	2 cut(s)	787, 1142
PciSI	GCTCTTC	3 cut(s)	1025, 3059, 3931
PcsI	WCGNNNNNNNCGW	1 cut(s)	1759
PctI	GAATGC	3 cut(s)	1803, 3842, 4222
PdmI	GAANNNNTTC	1 cut(s)	3547
PflMI	CCANNNNNTGG	1 cut(s)	4167
PfoI	TCCNGGA	1 cut(s)	2221
PinAI	ACCGGT	1 cut(s)	1123
PleI	GAGTC	4 cut(s)	395, 610, 3829, 3860
PluTI	GGCGCC	1 cut(s)	1248
PpsI	GAGTC	4 cut(s)	395, 610, 3829, 3860
PpuMI	RGGWCCY	5 cut(s)	1168, 2938, 3748, 3755, 3790
PscI	ACATGT	2 cut(s)	787, 1142
PshBI	ATTAAT	1 cut(s)	681
Psp5II	RGGWCCY	5 cut(s)	1168, 2938, 3748, 3755, 3790
PspOMI	GGGCCC	1 cut(s)	2348
PspPPI	RGGWCCY	5 cut(s)	1168, 2938, 3748, 3755, 3790
PstI	CTGCAG	2 cut(s)	265, 1407
PsuI	RGATCY	5 cut(s)	859, 1363, 1407, 2455, 3325
PvuII	CAGCTG	4 cut(s)	266, 317, 1310, 3292
RsaI	GTAC	8 cut(s)	520, 641, 786, 869, 1716, 2148, 3250, 3904
RsaNI	GTAC	8 cut(s)	519, 640, 785, 868, 1715, 2147, 3249, 3903
RseI	CAYNNNNRTG	4 cut(s)	786, 1604, 3240, 3851
SapI	GCTCTTC	3 cut(s)	1025, 3059, 3931
ScaI	AGTACT	3 cut(s)	869, 2148, 3250
SchI	GAGTC	4 cut(s)	395, 610, 3830, 3860
SduI	GDGCHC	8 cut(s)	113, 181, 465, 1037, 1346, 1464, 2352, 3007
SfcI	CTRYAG	5 cut(s)	261, 1215, 1305, 1403, 2607
SfoI	GGCGCC	1 cut(s)	1246
SinI	GGWCC	7 cut(s)	539, 1168, 1759, 2938, 3748, 3755, 3790
SmaI	CCCGGG	1 cut(s)	3753
SmiMI	CAYNNNNRTG	4 cut(s)	786, 1604, 3240, 3851
SmlI	CTYRAG	6 cut(s)	180, 326, 1084, 2246, 2783, 3759
SmoI	CTYRAG	6 cut(s)	180, 326, 1084, 2246, 2783, 3759
SpeI	ACTAGT	1 cut(s)	3281
SspDI	GGCGCC	1 cut(s)	1244
StyI	CCWWGG	6 cut(s)	542, 1355, 1860, 2673, 3509, 4062
TaiI	ACGT	4 cut(s)	1040, 2056, 2904, 4050
TaqI	TCGA	9 cut(s)	584, 592, 709, 1255, 1667, 2127, 2631, 3445, 3640
TatI	WGTACW	7 cut(s)	518, 784, 867, 1714, 2146, 3248, 3902
TauI	GCSGC	5 cut(s)	26, 29, 1869, 1980, 3688
TscAI	CASTG	6 cut(s)	386, 702, 952, 968, 2238, 3856
TseFI	GTSAC	2 cut(s)	613, 2518
Tsp45I	GTSAC	2 cut(s)	613, 2518
TspDTI	ATGAA	8 cut(s)	656, 692, 786, 1796, 3354, 3414, 3540, 3799
TspGWI	ACGGA	4 cut(s)	1356, 1751, 3375, 3554
TspMI	CCCGGG	1 cut(s)	3751
TspRI	CASTG	6 cut(s)	386, 702, 952, 968, 2238, 3856
Van91I	CCANNNNNTGG	1 cut(s)	4167
Vha464I	CTTAAG	1 cut(s)	2246
VpaK11BI	GGWCC	7 cut(s)	539, 1168, 1759, 2938, 3748, 3755, 3790
VspI	ATTAAT	1 cut(s)	681
XapI	RAATTY	7 cut(s)	469, 1387, 1451, 2177, 2358, 3078, 3560
XbaI	TCTAGA	1 cut(s)	3328
XceI	RCATGY	4 cut(s)	600, 791, 1146, 2421
XmaI	CCCGGG	1 cut(s)	3751
XmaJI	CCTAGG	1 cut(s)	542
XmiI	GTMKAC	1 cut(s)	2304
XmnI	GAANNNNTTC	1 cut(s)	3547
ZrmI	AGTACT	3 cut(s)	869, 2148, 3250

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.