AT1G75380

Bifunctional nuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0003171
Physical Location & Seq
Reverse (-)
28281195 .. 28284168
2974 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G75380.2

Sequence Viewer

Length: 978 bp
ATGAGATCGGTTCAAGCACCAGTTGTTTGTCCTGCAATTCGACCAAGACAAGTAGGTGCATGTGCTTCACTAGTCAACTACACTGGTCTGAAACCTAGAAGCCAATTTTGGGGTAATCGGACAAAAGGTGTCAAGTCTCAGGGCACTACCACAACAATCACTCTTAGATTGTGCAACAAGAGTATCAAGTGTGTTTTCAGCTCTCACTCTGATGGTAATGGAAGCACTGCTGAAAATTTCAATGAAAATGATGAGGAATATGTTAACTCTAGTGTAGTGGAAGCTGTTGAAGTAAAAAGTGGAGCAGATGGTTTTATGGTGAAGATGAGGGATGGTAGGCAACTACGATGTGTCCATAACAATCCTCAAGGAGGGCATTTACCAGATTATGCTCCACACCCTGCTATTGTCTTGAAAATGGAAGATGGAACTGGTCTTCTCCTTCCGATTATCGTCTTGGAGATGCCTAGTGTGTTACTTATGGCAGCAATGACCAATGTCCAAATTGCAAGACCAACCATGTATCAAGTGGTGAAGGAAATGGTGGACAAGATGGGTTACGAAGTTAGACTTGTTAGAGTCACCAAAAGAGTTCACGAGGCATATTTTGCCCAGTTATTCCTTTCAAAGGTGGGTAATGCCTCGGAGTGTGTCAGCTTTGATCTTAGGCCTTCAGATGCAATCAATATAGCTGTTAGATGCAAGATACCTATCCAAGTGAACAAGTACCTTGCATATAGTGATGGAATGAGAGTCATTGAGTCAGGGAAGATATCAACACCGGCACCAGCTTCAGATGGTTTATTATTTACTGAACAAGACCGACCAAATGGTCAGGCTTGTCTTGATACGAAAGAGTTCAATATTTTGAGCAAAATGATGCAAGCCGTTGATGAAGAGCGATATGATGAAGCCGCTGAGTGGAGAGACAAGCTTGGTCAGTTTCGGGCCAAACGTAACTTGAGGAAATACACATGA

Protein Analysis

325

Amino Acids

36.17

Weight (kDa)

8.93

Isoelectric Point (pI)

40.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BFN_dom PF02577 136 - 248 6.7e-21 Bifunctional nuclease domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 831
AccB1I GGYRCC 1 cut(s) 784
AciI CCGC 1 cut(s) 915
AcsI RAATTY 1 cut(s) 235
AcuI CTGAAG 2 cut(s) 657, 777
AfaI GTAC 1 cut(s) 728
AfiI CCNNNNNNNGG 4 cut(s) 109, 371, 628, 921
AgsI TTSAA 6 cut(s) 14, 241, 290, 415, 627, 862
AhlI ACTAGT 1 cut(s) 70
AjuI GAANNNNNNNTTGG 2 cut(s) 91, 123
AluBI AGCT 6 cut(s) 201, 284, 657, 692, 791, 934
AluI AGCT 6 cut(s) 201, 284, 657, 692, 791, 934
Alw26I GTCTC 2 cut(s) 141, 921
AoxI GGCC 2 cut(s) 668, 948
ApeKI GCWGC 1 cut(s) 485
ApoI RAATTY 1 cut(s) 235
Asp700I GAANNNNTTC 1 cut(s) 857
AspS9I GGNCC 1 cut(s) 948
AsuHPI GGTGA 3 cut(s) 331, 544, 574
BaeGI GKGCMC 1 cut(s) 146
BaeI ACNNNNGTAYC 2 cut(s) 166, 199
BanI GGYRCC 1 cut(s) 784
BauI CACGAG 1 cut(s) 596
BbsI GAAGAC 1 cut(s) 428
BbvI GCAGC 1 cut(s) 497
BccI CCATC 7 cut(s) 206, 302, 326, 419, 547, 737, 791
BceAI ACGGC 1 cut(s) 872
BcoDI GTCTC 2 cut(s) 141, 921
BcuI ACTAGT 1 cut(s) 70
BfaI CTAG 4 cut(s) 71, 96, 270, 468
BisI GCNGC 2 cut(s) 486, 915
BlsI GCNGC 2 cut(s) 487, 916
BmgT120I GGNCC 1 cut(s) 948
BmiI GGNNCC 1 cut(s) 786
BmrI ACTGGG 1 cut(s) 607
BmsI GCATC 4 cut(s) 453, 667, 689, 870
BmuI ACTGGG 1 cut(s) 607
BoxI GACNNNNGTC 1 cut(s) 497
BpiI GAAGAC 1 cut(s) 428
BpuEI CTTGAG 1 cut(s) 351
BsaBI GATNNNNATC 1 cut(s) 710
BsaJI CCNNGG 1 cut(s) 642
Bsc4I CCNNNNNNNGG 4 cut(s) 109, 371, 628, 921
Bse118I RCCGGY 1 cut(s) 781
Bse1I ACTGG 4 cut(s) 20, 88, 436, 613
Bse3DI GCAATG 1 cut(s) 495
Bse8I GATNNNNATC 1 cut(s) 710
BseDI CCNNGG 1 cut(s) 642
BseGI GGATG 1 cut(s) 337
BseJI GATNNNNATC 1 cut(s) 710
BseLI CCNNNNNNNGG 4 cut(s) 109, 371, 628, 921
BseMI GCAATG 1 cut(s) 495
BseMII CTCAG 2 cut(s) 152, 909
BseNI ACTGG 4 cut(s) 20, 88, 436, 613
BseSI GKGCMC 1 cut(s) 146
BseXI GCAGC 1 cut(s) 497
BshFI GGCC 2 cut(s) 670, 950
BshNI GGYRCC 1 cut(s) 784
BsiSI CCGG 1 cut(s) 782
BslI CCNNNNNNNGG 4 cut(s) 109, 371, 628, 921
BsmAI GTCTC 2 cut(s) 141, 921
BsnI GGCC 2 cut(s) 670, 950
Bsp1286I GDGCHC 1 cut(s) 146
Bsp143I GATC 2 cut(s) 5, 661
BspACI CCGC 1 cut(s) 915
BspANI GGCC 2 cut(s) 670, 950
BspCNI CTCAG 2 cut(s) 151, 910
BspLI GGNNCC 1 cut(s) 786
BspQI GCTCTTC 1 cut(s) 891
BspT107I GGYRCC 1 cut(s) 784
BsrDI GCAATG 1 cut(s) 495
BsrFI RCCGGY 1 cut(s) 781
BsrI ACTGG 4 cut(s) 20, 88, 436, 613
BssAI RCCGGY 1 cut(s) 781
BssECI CCNNGG 1 cut(s) 642
BssMI GATC 2 cut(s) 5, 661
BssSI CACGAG 1 cut(s) 596
Bst2BI CACGAG 1 cut(s) 596
Bst6I CTCTTC 1 cut(s) 891
BstAPI GCANNNNNTGC 1 cut(s) 608
BstC8I GCNNGC 1 cut(s) 885
BstDEI CTNAG 4 cut(s) 138, 164, 665, 918
BstENI CCTNNNNNAGG 2 cut(s) 369, 626
BstF5I GGATG 1 cut(s) 337
BstKTI GATC 2 cut(s) 8, 664
BstMAI GTCTC 2 cut(s) 141, 921
BstMBI GATC 2 cut(s) 5, 661
BstMWI GCNNNNNNNGC 1 cut(s) 608
BstNSI RCATGY 1 cut(s) 63
BstPAI GACNNNNGTC 1 cut(s) 497
BstSLI GKGCMC 1 cut(s) 146
BstV1I GCAGC 1 cut(s) 497
BstV2I GAAGAC 1 cut(s) 428
BsuRI GGCC 2 cut(s) 670, 950
BtsCI GGATG 1 cut(s) 337
BtsI GCAGTG 1 cut(s) 225
BtsIMutI CAGTG 2 cut(s) 81, 225
Cac8I GCNNGC 1 cut(s) 885
Cfr10I RCCGGY 1 cut(s) 781
Cfr13I GGNCC 1 cut(s) 948
Csp6I GTAC 1 cut(s) 727
CviAII CATG 3 cut(s) 60, 520, 975
CviQI GTAC 1 cut(s) 727
DdeI CTNAG 4 cut(s) 138, 164, 665, 918
DpnI GATC 2 cut(s) 7, 663
DpnII GATC 2 cut(s) 5, 661
DrdI GACNNNNNNGTC 1 cut(s) 831
DseDI GACNNNNNNGTC 1 cut(s) 831
Eam1104I CTCTTC 1 cut(s) 891
EarI CTCTTC 1 cut(s) 891
Eco147I AGGCCT 1 cut(s) 670
Eco32I GATATC 1 cut(s) 774
Eco57I CTGAAG 2 cut(s) 657, 777
EcoNI CCTNNNNNAGG 2 cut(s) 369, 626
EcoRV GATATC 1 cut(s) 774
FaeI CATG 3 cut(s) 63, 523, 978
FalI AAGNNNNNCTT 2 cut(s) 555, 587
FatI CATG 3 cut(s) 59, 519, 974
Fnu4HI GCNGC 2 cut(s) 486, 915
FokI GGATG 1 cut(s) 344
Fsp4HI GCNGC 2 cut(s) 486, 915
FspBI CTAG 4 cut(s) 71, 96, 270, 468
GluI GCNGC 2 cut(s) 486, 915
HaeIII GGCC 2 cut(s) 670, 950
HapII CCGG 1 cut(s) 782
Hin1II CATG 3 cut(s) 63, 523, 978
HincII GTYRAC 2 cut(s) 76, 265
HindII GTYRAC 2 cut(s) 76, 265
HindIII AAGCTT 1 cut(s) 932
HinfI GANTC 3 cut(s) 579, 753, 761
HpaI GTTAAC 1 cut(s) 265
HpaII CCGG 1 cut(s) 782
HphI GGTGA 3 cut(s) 331, 544, 574
Hpy166II GTNNAC 5 cut(s) 76, 265, 547, 595, 721
Hpy188I TCNGA 7 cut(s) 90, 120, 211, 447, 646, 676, 796
Hpy188III TCNNGA 3 cut(s) 412, 596, 845
Hpy8I GTNNAC 5 cut(s) 76, 265, 547, 595, 721
HpyAV CCTTC 3 cut(s) 452, 529, 681
HpyCH4IV ACGT 1 cut(s) 955
HpyCH4V TGCA 8 cut(s) 35, 59, 174, 509, 680, 702, 734, 883
HpyF10VI GCNNNNNNNGC 1 cut(s) 608
HpyF3I CTNAG 4 cut(s) 138, 164, 665, 918
HpySE526I ACGT 1 cut(s) 955
Hsp92II CATG 3 cut(s) 63, 523, 978
KspAI GTTAAC 1 cut(s) 265
Kzo9I GATC 2 cut(s) 5, 661
LguI GCTCTTC 1 cut(s) 891
LmnI GCTCC 2 cut(s) 302, 397
Lsp1109I GCAGC 1 cut(s) 497
LweI GCATC 4 cut(s) 453, 667, 689, 870
MaeI CTAG 4 cut(s) 71, 96, 270, 468
MaeII ACGT 1 cut(s) 955
MaeIII GTNAC 4 cut(s) 474, 557, 580, 956
MalI GATC 2 cut(s) 7, 663
MboI GATC 2 cut(s) 5, 661
MboII GAAGA 5 cut(s) 334, 428, 434, 781, 908
MhlI GDGCHC 1 cut(s) 146
MluCI AATT 4 cut(s) 36, 104, 235, 504
MlyI GAGTC 3 cut(s) 588, 762, 770
MnlI CCTC 7 cut(s) 247, 321, 365, 375, 592, 652, 957
MroXI GAANNNNTTC 1 cut(s) 857
MseI TTAA 1 cut(s) 264
MslI CAYNNNNRTG 1 cut(s) 210
MspA1I CMGCKG 1 cut(s) 917
MspI CCGG 1 cut(s) 782
MwoI GCNNNNNNNGC 1 cut(s) 608
NdeII GATC 2 cut(s) 5, 661
NlaIII CATG 3 cut(s) 63, 523, 978
NlaIV GGNNCC 1 cut(s) 786
NmuCI GTSAC 1 cut(s) 580
NspI RCATGY 1 cut(s) 63
PceI AGGCCT 1 cut(s) 670
PciSI GCTCTTC 1 cut(s) 891
PcsI WCGNNNNNNNCGW 1 cut(s) 952
PdmI GAANNNNTTC 1 cut(s) 857
PkrI GCNGC 2 cut(s) 487, 916
PleI GAGTC 3 cut(s) 587, 761, 769
PpsI GAGTC 3 cut(s) 587, 761, 769
PshAI GACNNNNGTC 1 cut(s) 497
PspN4I GGNNCC 1 cut(s) 786
PspPI GGNCC 1 cut(s) 948
RsaI GTAC 1 cut(s) 728
RsaNI GTAC 1 cut(s) 727
RseI CAYNNNNRTG 1 cut(s) 210
SapI GCTCTTC 1 cut(s) 891
SaqAI TTAA 1 cut(s) 264
SatI GCNGC 2 cut(s) 486, 915
Sau3AI GATC 2 cut(s) 5, 661
Sau96I GGNCC 1 cut(s) 948
SchI GAGTC 3 cut(s) 588, 762, 770
SduI GDGCHC 1 cut(s) 146
SfaNI GCATC 4 cut(s) 453, 667, 689, 870
SmiMI CAYNNNNRTG 1 cut(s) 210
SmlI CTYRAG 2 cut(s) 366, 961
SmoI CTYRAG 2 cut(s) 366, 961
SpeI ACTAGT 1 cut(s) 70
Sse9I AATT 4 cut(s) 36, 104, 235, 504
SseBI AGGCCT 1 cut(s) 670
SsiI CCGC 1 cut(s) 915
SspI AATATT 1 cut(s) 865
SspMI CTAG 4 cut(s) 71, 96, 270, 468
StuI AGGCCT 1 cut(s) 670
TaiI ACGT 1 cut(s) 958
TaqI TCGA 1 cut(s) 40
TaqII GACCGA 1 cut(s) 837
TasI AATT 4 cut(s) 36, 104, 235, 504
TauI GCSGC 1 cut(s) 917
Tru1I TTAA 1 cut(s) 264
Tru9I TTAA 1 cut(s) 264
TscAI CASTG 2 cut(s) 88, 232
TseFI GTSAC 1 cut(s) 580
TseI GCWGC 1 cut(s) 485
Tsp45I GTSAC 1 cut(s) 580
TspDTI ATGAA 3 cut(s) 258, 909, 924
TspRI CASTG 2 cut(s) 88, 232
XagI CCTNNNNNAGG 2 cut(s) 369, 626
XapI RAATTY 1 cut(s) 235
XceI RCATGY 1 cut(s) 63
XcmI CCANNNNNNNNNTGG 1 cut(s) 526
XmnI GAANNNNTTC 1 cut(s) 857
XspI CTAG 4 cut(s) 71, 96, 270, 468
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.