MD15G1023200.v1.1

Bifunctional nuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0003171
Physical Location & Seq
Forward (+)
1355594 .. 1359166
3573 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1023200.v1.1.491

Sequence Viewer

Length: 1110 bp
ATGATGGCGACTTTGCAAGGGCCGGTAATTTGCCCCACCGTTCGCGCAAAACAATCGGGGTTTGACACTCTGCCTGTGGTAGGCCCTTTGGTGAAGGCTAGGCTTGGATTCAGAGGGATCAGCGGCAGCATCACGAAGGCGGGTTTTTCTTCTCGCTCTCGAAATGCTAGAAAATGCTCAAGAGTGCATTGCATTTTCAGTTCGTCGTCGGATGGCAACGGAAGCATGGCGGAGAATTTCAATGAAAATGATGAAGATTATGTTAACTCTAGCGTACTCGAAGCCGTCGAGGTGAAGAGCGGAATTGATGGATTCATGATCAAAATGCGGGATGGTAGGCACATGAGGTGTGTCCATAACAATCCTCAGGGGGGCCATCTCCCCGATTATGCTCCGCATCCTGCAATTGTATTGAAGATGGAAGACGGGACCGGTCTTCTTCTCCCAATAATTGTTTTGGAGATGCCTAGTGTGTTGCTCATGGCAGCGGTCCGCAATGTTCCTATTGCTAGGCCAACTATGTATCAAGTGGTGAGGGAGATGATTGACAAGATGGGTTATGAGGTCCGACTTGTCAGAGTCACCAAGAGAGTGCATGAGGCGTATTTTGCTCAGTTATACCTCAGAAAGGTAGGTAGTGAGACAGAATGTGTCAGCTTCGACCTTCGGCCTTCAGATGCCATCAACATTGCTGTACGATGCAAGGTTCCCATACAGGTCAACAAGTACCTGGCATACAGTGATGGAATGAGAGTTATCGAATCTGGAAAGTTCTCAACTCAGGGCCCTGCTTCAGATGGATTATTATTTACTGAACTGGATCGGCCAAATGGTCAACCATGTGTTGAAACGAAGGAGTTTAATCTCGTGCGCAACATGCTTATTGCTGCAGTTGAGGAACGCTATAGAGATGCTGGTAAATATGTTTCGTCCCTCAGTGGAGGGACAAGCTCCTCCAACTTCGTGCTAGAAAGAACTTGGCATGACAGAATCGCCCGTAGGGATTTAAAATTGTACAGACAGCATTCTTGGAAGTTTGATAAGCTCATCGGCTGCACCCGCAATCCGCCATGCATCTCTGCTCTTCTTATATATATATATATGATGTAA

Protein Analysis

370

Amino Acids

41.22

Weight (kDa)

9.22

Isoelectric Point (pI)

42.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BFN_dom PF02577 136 - 247 1e-20 Bifunctional nuclease domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 872
AccBSI CCGCTC 1 cut(s) 300
AccII CGCG 1 cut(s) 45
AclWI GGATC 2 cut(s) 125, 828
AcoI YGGCCR 1 cut(s) 824
AcsI RAATTY 1 cut(s) 235
AcuI CTGAAG 2 cut(s) 657, 777
AfaI GTAC 4 cut(s) 276, 696, 728, 1016
AfiI CCNNNNNNNGG 3 cut(s) 80, 371, 628
AgeI ACCGGT 1 cut(s) 431
AgsI TTSAA 3 cut(s) 241, 415, 848
AjnI CCWGG 1 cut(s) 729
AluBI AGCT 3 cut(s) 657, 951, 1045
AluI AGCT 3 cut(s) 657, 951, 1045
Alw26I GTCTC 1 cut(s) 635
AlwI GGATC 2 cut(s) 125, 828
AoxI GGCC 7 cut(s) 20, 82, 373, 512, 668, 784, 824
ApaI GGGCCC 1 cut(s) 788
ApeKI GCWGC 4 cut(s) 126, 485, 887, 1053
ApoI RAATTY 1 cut(s) 235
AsiGI ACCGGT 1 cut(s) 431
AspLEI GCGC 2 cut(s) 47, 873
AspS9I GGNCC 8 cut(s) 20, 83, 373, 429, 490, 565, 784, 785
AsuHPI GGTGA 4 cut(s) 103, 304, 544, 574
AvaII GGWCC 3 cut(s) 429, 490, 565
AxyI CCTNAGG 1 cut(s) 366
BaeGI GKGCMC 1 cut(s) 788
BanII GRGCYC 1 cut(s) 788
BauI CACGAG 1 cut(s) 866
BbsI GAAGAC 2 cut(s) 428, 429
BbvI GCAGC 4 cut(s) 138, 497, 874, 1040
BccI CCATC 9 cut(s) 206, 302, 326, 384, 412, 547, 689, 737, 791
BceAI ACGGC 1 cut(s) 269
BcgI CGANNNNNNTGC 2 cut(s) 36, 70
BciT130I CCWGG 1 cut(s) 731
BclI TGATCA 1 cut(s) 318
BcoDI GTCTC 1 cut(s) 635
BfaI CTAG 6 cut(s) 99, 168, 270, 468, 510, 968
BfmI CTRYAG 2 cut(s) 888, 904
BisI GCNGC 5 cut(s) 124, 127, 486, 888, 1054
BlsI GCNGC 5 cut(s) 125, 128, 487, 889, 1055
Bme1390I CCNGG 1 cut(s) 731
Bme18I GGWCC 3 cut(s) 429, 490, 565
BmgT120I GGNCC 8 cut(s) 20, 83, 373, 429, 490, 565, 784, 785
BmiI GGNNCC 4 cut(s) 374, 430, 708, 786
BmrFI CCNGG 1 cut(s) 731
BmsI GCATC 7 cut(s) 138, 406, 453, 667, 689, 901, 1083
BpiI GAAGAC 2 cut(s) 428, 429
BpuEI CTTGAG 1 cut(s) 163
BsaWI WCCGGW 1 cut(s) 431
Bsc4I CCNNNNNNNGG 3 cut(s) 80, 371, 628
Bse118I RCCGGY 2 cut(s) 22, 431
Bse1I ACTGG 1 cut(s) 822
Bse21I CCTNAGG 1 cut(s) 366
Bse3DI GCAATG 3 cut(s) 187, 502, 687
BseBI CCWGG 1 cut(s) 731
BseGI GGATG 3 cut(s) 217, 337, 397
BseLI CCNNNNNNNGG 3 cut(s) 80, 371, 628
BseMI GCAATG 3 cut(s) 187, 502, 687
BseMII CTCAG 5 cut(s) 380, 626, 637, 794, 949
BseNI ACTGG 1 cut(s) 822
BseRI GAGGAG 1 cut(s) 943
BseSI GKGCMC 1 cut(s) 788
BseXI GCAGC 4 cut(s) 138, 497, 874, 1040
BsgI GTGCAG 1 cut(s) 1039
Bsh1236I CGCG 1 cut(s) 45
BshFI GGCC 7 cut(s) 22, 84, 375, 514, 670, 786, 826
BshTI ACCGGT 1 cut(s) 431
BsiSI CCGG 2 cut(s) 23, 432
BslFI GGGAC 3 cut(s) 442, 916, 958
BslI CCNNNNNNNGG 3 cut(s) 80, 371, 628
BsmAI GTCTC 1 cut(s) 635
BsmFI GGGAC 3 cut(s) 442, 916, 958
BsmI GAATGC 1 cut(s) 1024
BsnI GGCC 7 cut(s) 22, 84, 375, 514, 670, 786, 826
Bsp120I GGGCCC 1 cut(s) 784
Bsp1286I GDGCHC 1 cut(s) 788
Bsp1407I TGTACA 1 cut(s) 1014
Bsp143I GATC 3 cut(s) 117, 318, 820
BspANI GGCC 7 cut(s) 22, 84, 375, 514, 670, 786, 826
BspCNI CTCAG 5 cut(s) 379, 625, 636, 793, 948
BspFNI CGCG 1 cut(s) 45
BspHI TCATGA 1 cut(s) 315
BspLI GGNNCC 4 cut(s) 374, 430, 708, 786
BspMAI CTGCAG 1 cut(s) 892
BspPI GGATC 2 cut(s) 125, 828
BspQI GCTCTTC 2 cut(s) 290, 1089
BsrBI CCGCTC 1 cut(s) 300
BsrDI GCAATG 3 cut(s) 187, 502, 687
BsrFI RCCGGY 2 cut(s) 22, 431
BsrGI TGTACA 1 cut(s) 1014
BsrI ACTGG 1 cut(s) 822
BssAI RCCGGY 2 cut(s) 22, 431
BssMI GATC 3 cut(s) 117, 318, 820
BssSI CACGAG 1 cut(s) 866
Bst2BI CACGAG 1 cut(s) 866
Bst2UI CCWGG 1 cut(s) 731
Bst4CI ACNGT 2 cut(s) 40, 740
Bst6I CTCTTC 2 cut(s) 290, 1089
BstAUI TGTACA 1 cut(s) 1014
BstDEI CTNAG 5 cut(s) 366, 612, 623, 780, 935
BstENI CCTNNNNNAGG 2 cut(s) 78, 626
BstF5I GGATG 3 cut(s) 217, 337, 397
BstFNI CGCG 1 cut(s) 45
BstHHI GCGC 2 cut(s) 47, 873
BstKTI GATC 3 cut(s) 120, 321, 823
BstMAI GTCTC 1 cut(s) 635
BstMBI GATC 3 cut(s) 117, 318, 820
BstMWI GCNNNNNNNGC 4 cut(s) 222, 608, 877, 1059
BstNI CCWGG 1 cut(s) 731
BstNSI RCATGY 1 cut(s) 880
BstSCI CCNGG 1 cut(s) 729
BstSFI CTRYAG 2 cut(s) 888, 904
BstSLI GKGCMC 1 cut(s) 788
BstUI CGCG 1 cut(s) 45
BstV1I GCAGC 4 cut(s) 138, 497, 874, 1040
BstV2I GAAGAC 2 cut(s) 428, 429
Bsu36I CCTNAGG 1 cut(s) 366
BsuRI GGCC 7 cut(s) 22, 84, 375, 514, 670, 786, 826
BtsCI GGATG 3 cut(s) 217, 337, 397
BtsIMutI CAGTG 2 cut(s) 745, 943
CciI TCATGA 1 cut(s) 315
CfoI GCGC 2 cut(s) 47, 873
Cfr10I RCCGGY 2 cut(s) 22, 431
Cfr13I GGNCC 8 cut(s) 20, 83, 373, 429, 490, 565, 784, 785
CpoI CGGWCCG 1 cut(s) 490
Csp6I GTAC 4 cut(s) 275, 695, 727, 1015
CspAI ACCGGT 1 cut(s) 431
CspI CGGWCCG 1 cut(s) 490
CviAII CATG 9 cut(s) 226, 316, 343, 481, 596, 840, 877, 983, 1071
CviQI GTAC 4 cut(s) 275, 695, 727, 1015
DdeI CTNAG 5 cut(s) 366, 612, 623, 780, 935
DpnI GATC 3 cut(s) 119, 320, 822
DpnII GATC 3 cut(s) 117, 318, 820
DraI TTTAAA 1 cut(s) 1008
EaeI YGGCCR 1 cut(s) 824
Eam1104I CTCTTC 2 cut(s) 290, 1089
EarI CTCTTC 2 cut(s) 290, 1089
EciI GGCGGA 2 cut(s) 245, 1056
Eco24I GRGCYC 1 cut(s) 788
Eco47I GGWCC 3 cut(s) 429, 490, 565
Eco57I CTGAAG 2 cut(s) 657, 777
Eco81I CCTNAGG 1 cut(s) 366
EcoNI CCTNNNNNAGG 2 cut(s) 78, 626
EcoO109I RGGNCCY 3 cut(s) 83, 784, 785
EcoRII CCWGG 1 cut(s) 729
EcoT22I ATGCAT 1 cut(s) 1076
EcoT38I GRGCYC 1 cut(s) 788
FaeI CATG 9 cut(s) 229, 319, 346, 484, 599, 843, 880, 986, 1074
FaqI GGGAC 3 cut(s) 442, 916, 958
FatI CATG 9 cut(s) 225, 315, 342, 480, 595, 839, 876, 982, 1070
FauI CCCGC 3 cut(s) 133, 321, 1067
FbaI TGATCA 1 cut(s) 318
Fnu4HI GCNGC 5 cut(s) 124, 127, 486, 888, 1054
FokI GGATG 3 cut(s) 224, 344, 384
FriOI GRGCYC 1 cut(s) 788
Fsp4HI GCNGC 5 cut(s) 124, 127, 486, 888, 1054
FspBI CTAG 6 cut(s) 99, 168, 270, 468, 510, 968
FspI TGCGCA 1 cut(s) 872
GlaI GCGC 2 cut(s) 46, 872
GluI GCNGC 5 cut(s) 124, 127, 486, 888, 1054
HaeIII GGCC 7 cut(s) 22, 84, 375, 514, 670, 786, 826
HapII CCGG 2 cut(s) 23, 432
HhaI GCGC 2 cut(s) 47, 873
Hin1II CATG 9 cut(s) 229, 319, 346, 484, 599, 843, 880, 986, 1074
Hin6I GCGC 2 cut(s) 45, 871
HinP1I GCGC 2 cut(s) 45, 871
HincII GTYRAC 3 cut(s) 265, 721, 836
HindII GTYRAC 3 cut(s) 265, 721, 836
HinfI GANTC 5 cut(s) 108, 312, 579, 761, 990
HpaI GTTAAC 1 cut(s) 265
HpaII CCGG 2 cut(s) 23, 432
HphI GGTGA 4 cut(s) 103, 304, 544, 574
Hpy166II GTNNAC 3 cut(s) 265, 721, 836
Hpy188I TCNGA 7 cut(s) 113, 211, 569, 578, 626, 676, 796
Hpy188III TCNNGA 5 cut(s) 133, 159, 180, 316, 765
Hpy8I GTNNAC 3 cut(s) 265, 721, 836
Hpy99I CGWCG 3 cut(s) 208, 211, 290
HpyAV CCTTC 5 cut(s) 88, 130, 674, 681, 847
HpyCH4III ACNGT 2 cut(s) 40, 740
HpyCH4V TGCA 9 cut(s) 16, 187, 192, 404, 595, 702, 890, 1056, 1074
HpyF10VI GCNNNNNNNGC 4 cut(s) 222, 608, 877, 1059
HpyF3I CTNAG 5 cut(s) 366, 612, 623, 780, 935
Hsp92II CATG 9 cut(s) 229, 319, 346, 484, 599, 843, 880, 986, 1074
HspAI GCGC 2 cut(s) 45, 871
Ksp22I TGATCA 1 cut(s) 318
KspAI GTTAAC 1 cut(s) 265
Kzo9I GATC 3 cut(s) 117, 318, 820
LguI GCTCTTC 2 cut(s) 290, 1089
LmnI GCTCC 2 cut(s) 397, 956
Lsp1109I GCAGC 4 cut(s) 138, 497, 874, 1040
LweI GCATC 7 cut(s) 138, 406, 453, 667, 689, 901, 1083
MaeI CTAG 6 cut(s) 99, 168, 270, 468, 510, 968
MaeIII GTNAC 1 cut(s) 580
MalI GATC 3 cut(s) 119, 320, 822
MbiI CCGCTC 1 cut(s) 300
MboI GATC 3 cut(s) 117, 318, 820
MboII GAAGA 8 cut(s) 141, 266, 307, 427, 428, 431, 434, 1076
MfeI CAATTG 1 cut(s) 405
MhlI GDGCHC 1 cut(s) 788
MluCI AATT 6 cut(s) 27, 235, 303, 405, 450, 1010
MlyI GAGTC 1 cut(s) 588
MmeI TCCRAC 3 cut(s) 189, 592, 981
Mph1103I ATGCAT 1 cut(s) 1076
MseI TTAA 3 cut(s) 264, 861, 1007
MspA1I CMGCKG 2 cut(s) 123, 488
MspI CCGG 2 cut(s) 23, 432
MspR9I CCNGG 1 cut(s) 731
MunI CAATTG 1 cut(s) 405
Mva1269I GAATGC 1 cut(s) 1024
MvaI CCWGG 1 cut(s) 731
MvnI CGCG 1 cut(s) 45
MwoI GCNNNNNNNGC 4 cut(s) 222, 608, 877, 1059
NdeII GATC 3 cut(s) 117, 318, 820
NlaIII CATG 9 cut(s) 229, 319, 346, 484, 599, 843, 880, 986, 1074
NlaIV GGNNCC 4 cut(s) 374, 430, 708, 786
NmuCI GTSAC 1 cut(s) 580
NsbI TGCGCA 1 cut(s) 872
NsiI ATGCAT 1 cut(s) 1076
NspI RCATGY 1 cut(s) 880
PagI TCATGA 1 cut(s) 315
PciSI GCTCTTC 2 cut(s) 290, 1089
PcsI WCGNNNNNNNCGW 1 cut(s) 285
PctI GAATGC 1 cut(s) 1024
PfeI GAWTC 4 cut(s) 108, 312, 761, 990
PinAI ACCGGT 1 cut(s) 431
PkrI GCNGC 5 cut(s) 125, 128, 487, 889, 1055
PleI GAGTC 1 cut(s) 587
PpsI GAGTC 1 cut(s) 587
Psp6I CCWGG 1 cut(s) 729
PspGI CCWGG 1 cut(s) 729
PspN4I GGNNCC 4 cut(s) 374, 430, 708, 786
PspOMI GGGCCC 1 cut(s) 784
PspPI GGNCC 8 cut(s) 20, 83, 373, 429, 490, 565, 784, 785
PstI CTGCAG 1 cut(s) 892
RsaI GTAC 4 cut(s) 276, 696, 728, 1016
RsaNI GTAC 4 cut(s) 275, 695, 727, 1015
Rsr2I CGGWCCG 1 cut(s) 490
RsrII CGGWCCG 1 cut(s) 490
SapI GCTCTTC 2 cut(s) 290, 1089
SaqAI TTAA 3 cut(s) 264, 861, 1007
SatI GCNGC 5 cut(s) 124, 127, 486, 888, 1054
Sau3AI GATC 3 cut(s) 117, 318, 820
Sau96I GGNCC 8 cut(s) 20, 83, 373, 429, 490, 565, 784, 785
SchI GAGTC 1 cut(s) 588
ScrFI CCNGG 1 cut(s) 731
SduI GDGCHC 1 cut(s) 788
SfaNI GCATC 7 cut(s) 138, 406, 453, 667, 689, 901, 1083
SfcI CTRYAG 2 cut(s) 888, 904
SinI GGWCC 3 cut(s) 429, 490, 565
SmlI CTYRAG 1 cut(s) 178
SmoI CTYRAG 1 cut(s) 178
Sse9I AATT 6 cut(s) 27, 235, 303, 405, 450, 1010
SspMI CTAG 6 cut(s) 99, 168, 270, 468, 510, 968
StyD4I CCNGG 1 cut(s) 729
TaaI ACNGT 2 cut(s) 40, 740
TaqI TCGA 5 cut(s) 160, 279, 288, 660, 759
TasI AATT 6 cut(s) 27, 235, 303, 405, 450, 1010
TatI WGTACW 1 cut(s) 1014
TauI GCSGC 1 cut(s) 126
TfiI GAWTC 4 cut(s) 108, 312, 761, 990
Tru1I TTAA 3 cut(s) 264, 861, 1007
Tru9I TTAA 3 cut(s) 264, 861, 1007
TscAI CASTG 2 cut(s) 745, 943
TseFI GTSAC 1 cut(s) 580
TseI GCWGC 4 cut(s) 126, 485, 887, 1053
Tsp45I GTSAC 1 cut(s) 580
TspDTI ATGAA 3 cut(s) 258, 267, 304
TspGWI ACGGA 1 cut(s) 234
TspRI CASTG 2 cut(s) 745, 943
VpaK11BI GGWCC 3 cut(s) 429, 490, 565
XagI CCTNNNNNAGG 2 cut(s) 78, 626
XapI RAATTY 1 cut(s) 235
XceI RCATGY 1 cut(s) 880
XspI CTAG 6 cut(s) 99, 168, 270, 468, 510, 968
Zsp2I ATGCAT 1 cut(s) 1076
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.