MD08G1026400.v1.1

Bifunctional nuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0003171
Physical Location & Seq
Forward (+)
1931736 .. 1935248
3513 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1026400.v1.1.491

Sequence Viewer

Length: 744 bp
ATGGCGGAGAATTTCAATGAAAATGACGAAGATTATGTCAACTCTAGCGTACTTGAAGCCGTTGAGGTGAAGAGTGGAATTGATGGATTCATGATCAAGATGCGGGATGGTAGGCACATGAGGTGTGTCCATAACAACCCTCAAGGGGGGCATCTCCCCGATTATGCTCCGCATCCTGCAATTGTATTGAAGATGGAGGATGGGACCGGTCTTCTTCTCCCAATAATTGTTTTGGAGATGCCTAGTGTGTTGCTCATGGCAGCAGTCCGCAATGTTCCTATTGCTAGGCCAACTATGTATCAAGTGGTGAAGGAGATGATTGACAAGATGGGTTATGAGGTCCGACTTGTCAGAGTCACCAAGAGAGTGCATGAGGCGTATTTTGCTCAGCTATACCTCAGAAAGGCAGGTAGTGAGACAGAGTGTGTCAGCTTTGACCTTCGACCTTCAGACGCCATCAACATTGCTGTACGGTGCAAGGTTCCCATACAGGTCAACAAGTACCTGGCATACAGTGATGGAATGAAAGTTATCGAATCTGGAAAGTTTTCCACTCAAGGCCCTGCTTCAGATGGATTATTATTTACTCAACTGGATCGGCCAAATGGTCAACCATGTGTTGAAACGAAGGAGTTTAATCTTGTGCGCAACATGCTTATTGCTGCAGTTGAGGAACGCTATAGAGATGCTGCTCAATGGAGGGACAAGCTCCTCCAACTTCGTGCTAGAAAGAACTTGGCATGA

Protein Analysis

248

Amino Acids

27.91

Weight (kDa)

7.67

Isoelectric Point (pI)

38.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BFN_dom PF02577 61 - 174 1.1e-21 Bifunctional nuclease domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 647
Acc36I ACCTGC 1 cut(s) 398
AciI CCGC 4 cut(s) 5, 103, 170, 268
AclWI GGATC 1 cut(s) 603
AcoI YGGCCR 1 cut(s) 599
AcsI RAATTY 1 cut(s) 10
AcuI CTGAAG 2 cut(s) 432, 552
AcyI GRCGYC 1 cut(s) 453
AfaI GTAC 3 cut(s) 51, 471, 503
AfiI CCNNNNNNNGG 3 cut(s) 145, 146, 403
AgeI ACCGGT 1 cut(s) 206
AgsI TTSAA 4 cut(s) 16, 56, 190, 623
AjnI CCWGG 1 cut(s) 504
AluBI AGCT 3 cut(s) 391, 432, 709
AluI AGCT 3 cut(s) 391, 432, 709
Alw26I GTCTC 1 cut(s) 410
AlwI GGATC 1 cut(s) 603
AoxI GGCC 3 cut(s) 287, 559, 599
ApeKI GCWGC 3 cut(s) 260, 662, 689
ApoI RAATTY 1 cut(s) 10
ArsI GACNNNNNNTTYG 2 cut(s) 21, 53
AsiGI ACCGGT 1 cut(s) 206
Asp700I GAANNNNTTC 1 cut(s) 547
AspLEI GCGC 1 cut(s) 648
AspS9I GGNCC 3 cut(s) 204, 340, 560
AsuHPI GGTGA 3 cut(s) 79, 319, 349
AvaII GGWCC 2 cut(s) 204, 340
BbsI GAAGAC 1 cut(s) 203
BbvI GCAGC 3 cut(s) 272, 649, 676
BccI CCATC 8 cut(s) 77, 101, 187, 194, 322, 464, 512, 566
BceAI ACGGC 1 cut(s) 44
BciT130I CCWGG 1 cut(s) 506
BclI TGATCA 1 cut(s) 93
BcoDI GTCTC 1 cut(s) 410
BfaI CTAG 4 cut(s) 45, 243, 285, 726
BfmI CTRYAG 2 cut(s) 663, 679
BfuAI ACCTGC 1 cut(s) 398
BisI GCNGC 3 cut(s) 261, 663, 690
BlpI GCTNAGC 1 cut(s) 387
BlsI GCNGC 3 cut(s) 262, 664, 691
Bme1390I CCNGG 1 cut(s) 506
Bme18I GGWCC 2 cut(s) 204, 340
BmgT120I GGNCC 3 cut(s) 204, 340, 560
BmiI GGNNCC 2 cut(s) 205, 483
BmrFI CCNGG 1 cut(s) 506
BmsI GCATC 5 cut(s) 90, 160, 181, 228, 676
BpiI GAAGAC 1 cut(s) 203
Bpu1102I GCTNAGC 1 cut(s) 387
BpuEI CTTGAG 2 cut(s) 126, 540
BsaHI GRCGYC 1 cut(s) 453
BsaWI WCCGGW 1 cut(s) 206
Bsc4I CCNNNNNNNGG 3 cut(s) 145, 146, 403
Bse118I RCCGGY 1 cut(s) 206
Bse1I ACTGG 1 cut(s) 597
Bse3DI GCAATG 2 cut(s) 277, 462
BseBI CCWGG 1 cut(s) 506
BseGI GGATG 3 cut(s) 112, 172, 205
BseLI CCNNNNNNNGG 3 cut(s) 145, 146, 403
BseMI GCAATG 2 cut(s) 277, 462
BseMII CTCAG 2 cut(s) 401, 412
BseNI ACTGG 1 cut(s) 597
BseRI GAGGAG 1 cut(s) 701
BseXI GCAGC 3 cut(s) 272, 649, 676
BshFI GGCC 3 cut(s) 289, 561, 601
BshTI ACCGGT 1 cut(s) 206
BsiSI CCGG 1 cut(s) 207
BslFI GGGAC 2 cut(s) 217, 716
BslI CCNNNNNNNGG 3 cut(s) 145, 146, 403
BsmAI GTCTC 1 cut(s) 410
BsmFI GGGAC 2 cut(s) 217, 716
BsnI GGCC 3 cut(s) 289, 561, 601
Bsp143I GATC 2 cut(s) 93, 595
Bsp1720I GCTNAGC 1 cut(s) 387
BspACI CCGC 4 cut(s) 5, 103, 170, 268
BspANI GGCC 3 cut(s) 289, 561, 601
BspCNI CTCAG 2 cut(s) 400, 411
BspHI TCATGA 1 cut(s) 90
BspLI GGNNCC 2 cut(s) 205, 483
BspMAI CTGCAG 1 cut(s) 667
BspMI ACCTGC 1 cut(s) 398
BspPI GGATC 1 cut(s) 603
BsrDI GCAATG 2 cut(s) 277, 462
BsrFI RCCGGY 1 cut(s) 206
BsrI ACTGG 1 cut(s) 597
BssAI RCCGGY 1 cut(s) 206
BssMI GATC 2 cut(s) 93, 595
BssNI GRCGYC 1 cut(s) 453
Bst2UI CCWGG 1 cut(s) 506
Bst4CI ACNGT 2 cut(s) 474, 515
Bst6I CTCTTC 1 cut(s) 65
BstACI GRCGYC 1 cut(s) 453
BstDEI CTNAG 2 cut(s) 387, 398
BstENI CCTNNNNNAGG 1 cut(s) 401
BstF5I GGATG 3 cut(s) 112, 172, 205
BstHHI GCGC 1 cut(s) 648
BstKTI GATC 2 cut(s) 96, 598
BstMAI GTCTC 1 cut(s) 410
BstMBI GATC 2 cut(s) 93, 595
BstMWI GCNNNNNNNGC 2 cut(s) 383, 652
BstNI CCWGG 1 cut(s) 506
BstNSI RCATGY 1 cut(s) 655
BstSCI CCNGG 1 cut(s) 504
BstSFI CTRYAG 2 cut(s) 663, 679
BstV1I GCAGC 3 cut(s) 272, 649, 676
BstV2I GAAGAC 1 cut(s) 203
BsuRI GGCC 3 cut(s) 289, 561, 601
BtsCI GGATG 3 cut(s) 112, 172, 205
BtsIMutI CAGTG 1 cut(s) 520
BveI ACCTGC 1 cut(s) 398
CciI TCATGA 1 cut(s) 90
CfoI GCGC 1 cut(s) 648
Cfr10I RCCGGY 1 cut(s) 206
Cfr13I GGNCC 3 cut(s) 204, 340, 560
CseI GACGC 1 cut(s) 461
Csp6I GTAC 3 cut(s) 50, 470, 502
CspAI ACCGGT 1 cut(s) 206
CviAII CATG 7 cut(s) 91, 118, 256, 371, 615, 652, 741
CviJI RGCY 7 cut(s) 59, 289, 391, 432, 561, 601, 709
CviKI_1 RGCY 7 cut(s) 59, 289, 391, 432, 561, 601, 709
CviQI GTAC 3 cut(s) 50, 470, 502
DdeI CTNAG 2 cut(s) 387, 398
DpnI GATC 2 cut(s) 95, 597
DpnII GATC 2 cut(s) 93, 595
EaeI YGGCCR 1 cut(s) 599
Eam1104I CTCTTC 1 cut(s) 65
EarI CTCTTC 1 cut(s) 65
EciI GGCGGA 1 cut(s) 20
Eco47I GGWCC 2 cut(s) 204, 340
Eco57I CTGAAG 2 cut(s) 432, 552
EcoNI CCTNNNNNAGG 1 cut(s) 401
EcoO109I RGGNCCY 1 cut(s) 560
EcoRII CCWGG 1 cut(s) 504
FaeI CATG 7 cut(s) 94, 121, 259, 374, 618, 655, 744
FaqI GGGAC 2 cut(s) 217, 716
FatI CATG 7 cut(s) 90, 117, 255, 370, 614, 651, 740
FauI CCCGC 1 cut(s) 96
FbaI TGATCA 1 cut(s) 93
Fnu4HI GCNGC 3 cut(s) 261, 663, 690
FokI GGATG 3 cut(s) 119, 159, 212
Fsp4HI GCNGC 3 cut(s) 261, 663, 690
FspBI CTAG 4 cut(s) 45, 243, 285, 726
FspI TGCGCA 1 cut(s) 647
GlaI GCGC 1 cut(s) 647
GluI GCNGC 3 cut(s) 261, 663, 690
HaeIII GGCC 3 cut(s) 289, 561, 601
HapII CCGG 1 cut(s) 207
HgaI GACGC 1 cut(s) 461
HhaI GCGC 1 cut(s) 648
Hin1I GRCGYC 1 cut(s) 453
Hin1II CATG 7 cut(s) 94, 121, 259, 374, 618, 655, 744
Hin6I GCGC 1 cut(s) 646
HinP1I GCGC 1 cut(s) 646
HincII GTYRAC 3 cut(s) 40, 496, 611
HindII GTYRAC 3 cut(s) 40, 496, 611
HinfI GANTC 3 cut(s) 87, 354, 536
HpaII CCGG 1 cut(s) 207
HphI GGTGA 3 cut(s) 79, 319, 349
Hpy166II GTNNAC 3 cut(s) 40, 496, 611
Hpy188I TCNGA 5 cut(s) 344, 353, 401, 451, 571
Hpy188III TCNNGA 3 cut(s) 91, 97, 540
Hpy8I GTNNAC 3 cut(s) 40, 496, 611
HpyAV CCTTC 4 cut(s) 304, 449, 456, 622
HpyCH4III ACNGT 2 cut(s) 474, 515
HpyCH4V TGCA 4 cut(s) 179, 370, 477, 665
HpyF10VI GCNNNNNNNGC 2 cut(s) 383, 652
HpyF3I CTNAG 2 cut(s) 387, 398
Hsp92I GRCGYC 1 cut(s) 453
Hsp92II CATG 7 cut(s) 94, 121, 259, 374, 618, 655, 744
HspAI GCGC 1 cut(s) 646
Ksp22I TGATCA 1 cut(s) 93
Kzo9I GATC 2 cut(s) 93, 595
LmnI GCTCC 2 cut(s) 172, 714
LpnPI CCDG 9 cut(s) 189, 220, 393, 476, 491, 518, 525, 576, 578
Lsp1109I GCAGC 3 cut(s) 272, 649, 676
LweI GCATC 5 cut(s) 90, 160, 181, 228, 676
MaeI CTAG 4 cut(s) 45, 243, 285, 726
MaeIII GTNAC 1 cut(s) 355
MalI GATC 2 cut(s) 95, 597
MboI GATC 2 cut(s) 93, 595
MboII GAAGA 5 cut(s) 41, 82, 202, 203, 206
MfeI CAATTG 1 cut(s) 180
MluCI AATT 4 cut(s) 10, 78, 180, 225
MlyI GAGTC 1 cut(s) 363
MmeI TCCRAC 2 cut(s) 367, 739
MroXI GAANNNNTTC 1 cut(s) 547
MseI TTAA 1 cut(s) 636
MspI CCGG 1 cut(s) 207
MspR9I CCNGG 1 cut(s) 506
MunI CAATTG 1 cut(s) 180
MvaI CCWGG 1 cut(s) 506
MwoI GCNNNNNNNGC 2 cut(s) 383, 652
NdeII GATC 2 cut(s) 93, 595
NlaIII CATG 7 cut(s) 94, 121, 259, 374, 618, 655, 744
NlaIV GGNNCC 2 cut(s) 205, 483
NmuCI GTSAC 1 cut(s) 355
NsbI TGCGCA 1 cut(s) 647
NspI RCATGY 1 cut(s) 655
PagI TCATGA 1 cut(s) 90
PdmI GAANNNNTTC 1 cut(s) 547
PfeI GAWTC 2 cut(s) 87, 536
PinAI ACCGGT 1 cut(s) 206
PkrI GCNGC 3 cut(s) 262, 664, 691
PleI GAGTC 1 cut(s) 362
PpsI GAGTC 1 cut(s) 362
Psp6I CCWGG 1 cut(s) 504
PspGI CCWGG 1 cut(s) 504
PspN4I GGNNCC 2 cut(s) 205, 483
PspPI GGNCC 3 cut(s) 204, 340, 560
PstI CTGCAG 1 cut(s) 667
RsaI GTAC 3 cut(s) 51, 471, 503
RsaNI GTAC 3 cut(s) 50, 470, 502
SaqAI TTAA 1 cut(s) 636
SatI GCNGC 3 cut(s) 261, 663, 690
Sau3AI GATC 2 cut(s) 93, 595
Sau96I GGNCC 3 cut(s) 204, 340, 560
SchI GAGTC 1 cut(s) 363
ScrFI CCNGG 1 cut(s) 506
SfaNI GCATC 5 cut(s) 90, 160, 181, 228, 676
SfcI CTRYAG 2 cut(s) 663, 679
SinI GGWCC 2 cut(s) 204, 340
SmlI CTYRAG 2 cut(s) 141, 555
SmoI CTYRAG 2 cut(s) 141, 555
Sse9I AATT 4 cut(s) 10, 78, 180, 225
SsiI CCGC 4 cut(s) 5, 103, 170, 268
SspMI CTAG 4 cut(s) 45, 243, 285, 726
StyD4I CCNGG 1 cut(s) 504
TaaI ACNGT 2 cut(s) 474, 515
TaqI TCGA 2 cut(s) 442, 534
TasI AATT 4 cut(s) 10, 78, 180, 225
TfiI GAWTC 2 cut(s) 87, 536
Tru1I TTAA 1 cut(s) 636
Tru9I TTAA 1 cut(s) 636
TscAI CASTG 1 cut(s) 520
TseFI GTSAC 1 cut(s) 355
TseI GCWGC 3 cut(s) 260, 662, 689
Tsp45I GTSAC 1 cut(s) 355
TspDTI ATGAA 3 cut(s) 33, 79, 539
TspRI CASTG 1 cut(s) 520
VpaK11BI GGWCC 2 cut(s) 204, 340
XagI CCTNNNNNAGG 1 cut(s) 401
XapI RAATTY 1 cut(s) 10
XceI RCATGY 1 cut(s) 655
XmnI GAANNNNTTC 1 cut(s) 547
XspI CTAG 4 cut(s) 45, 243, 285, 726
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.