AT2G02450
NAC Family

NAC domain containing protein 35

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0010263
Physical Location & Seq
Forward (+)
647538 .. 650820
3283 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G02450.2

Sequence Viewer

Length: 1140 bp
ATGGCAATTGTATCCTCCACAACAAGCATCATTCCCATGAGTAACCAAGTCAACAATAACGAAAAAGGTATAGAAGACAATGATCATAGAGGCGGCCAAGAGAGTCATGTCCAAAATGAAGATGAAGCTGATGATCATGATCATGACATGGTCATGCCCGGATTTAGATTCCATCCTACCGAAGAAGAACTCATAGAGTTTTACCTTCGCCGAAAAGTTGAAGGCAAACGCTTTAATGTAGAACTCATCACTTTCCTCGATCTTTATCGCTATGATCCTTGGGAACTTCCTGCTATGGCGGCGATAGGAGAGAAAGAGTGGTACTTCTATGTGCCAAGAGATCGGAAATATAGAAATGGAGATAGACCGAACCGAGTAACGACTTCAGGATATTGGAAAGCCACCGGAGCTGATAGGATGATCAGATCGGAGACTTCTCGGCCTATCGGATTAAAGAAAACCCTAGTTTTCTACTCTGGTAAAGCCCCTAAAGGCACTCGTACTAGTTGGATCATGAACGAGTATCGTCTTCCGCACCATGAAACCGAGAAGTACCAAAAGGCTGAAATATCATTGTGCCGAGTGTACAAAAGGCCAGGAGTAGAAGATCATCCATCGGTACCACGTTCTCTCTCCACAAGACATCATAACCATAACTCATCGACATCATCCCGTTTAGCCTTAAGACAACAACAACACCATTCATCCTCCTCTAATCATTCCGACAACAACCTTAACAACAACAACAACATCAACAATCTCGAGAAGCTCTCCACCGAATATTCCGGCGACGGCAGCACAACAACAACGACCACAAACAGTAACTCTGACGTTACCATTGCTCTAGCCAATCAAAACATATATCGTCCAATGCCTTACGACACAAGCAACAACACATTGATAGTCTCTACGAGAAATCATCAAGACGATGATGAAACTGCCATTGTTGACGATCTTCAAAGACTAGTTAACTACCAAATATCAGATGGAGCGACAACGCTAATGCCTCAAACTCAAGCGGCGTTAGCTATGAACATGATTCCTGCAGGAACGATTCCAAACAATGCTTTGTGGGATATGTGGAATCCAATAGTACCAGATGGAAACAGAGATCACTATACTAATATTCCTTTTAAGTAA

Protein Analysis

379

Amino Acids

43.43

Weight (kDa)

6.06

Isoelectric Point (pI)

43.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM PF02365 53 - 177 5.8e-46 No apical meristem (NAM) protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 619
AccB1I GGYRCC 1 cut(s) 619
AciI CCGC 4 cut(s) 93, 299, 533, 1019
AclWI GGATC 2 cut(s) 269, 518
AcoI YGGCCR 1 cut(s) 94
AcuI CTGAAG 1 cut(s) 369
AfaI GTAC 6 cut(s) 323, 502, 554, 587, 621, 1095
AflII CTTAAG 1 cut(s) 682
AgsI TTSAA 2 cut(s) 221, 959
AhlI ACTAGT 2 cut(s) 503, 964
AjnI CCWGG 1 cut(s) 595
AluBI AGCT 4 cut(s) 128, 410, 769, 1028
AluI AGCT 4 cut(s) 128, 410, 769, 1028
Alw26I GTCTC 2 cut(s) 425, 910
AlwI GGATC 2 cut(s) 269, 518
Ama87I CYCGRG 1 cut(s) 761
AoxI GGCC 3 cut(s) 94, 440, 593
ApeKI GCWGC 1 cut(s) 795
Asp718I GGTACC 1 cut(s) 619
AsuC2I CCSGG 1 cut(s) 159
AvaI CYCGRG 1 cut(s) 761
BanI GGYRCC 1 cut(s) 619
BbsI GAAGAC 2 cut(s) 81, 521
BbvI GCAGC 1 cut(s) 807
BccI CCATC 4 cut(s) 180, 622, 980, 1094
BceAI ACGGC 1 cut(s) 808
BciT130I CCWGG 1 cut(s) 597
BciVI GTATCC 1 cut(s) 22
BclI TGATCA 4 cut(s) 82, 133, 139, 420
BcnI CCSGG 1 cut(s) 159
BcoDI GTCTC 2 cut(s) 425, 910
BcuI ACTAGT 2 cut(s) 503, 964
BfaI CTAG 4 cut(s) 464, 504, 845, 965
BfmI CTRYAG 1 cut(s) 1044
BfrI CTTAAG 1 cut(s) 682
BfuI GTATCC 1 cut(s) 22
BisI GCNGC 4 cut(s) 94, 300, 796, 1020
BlsI GCNGC 4 cut(s) 95, 301, 797, 1021
Bme1390I CCNGG 2 cut(s) 159, 597
BmeT110I CYCGRG 1 cut(s) 761
BmiI GGNNCC 1 cut(s) 621
BmrFI CCNGG 2 cut(s) 159, 597
BmsI GCATC 1 cut(s) 36
BpiI GAAGAC 2 cut(s) 81, 521
BplI GAGNNNNNCTC 2 cut(s) 755, 787
BpuEI CTTGAG 1 cut(s) 999
BpuMI CCSGG 1 cut(s) 159
BsaBI GATNNNNATC 2 cut(s) 138, 264
BsaJI CCNNGG 1 cut(s) 278
BsaWI WCCGGW 1 cut(s) 404
Bse3DI GCAATG 1 cut(s) 837
Bse8I GATNNNNATC 2 cut(s) 138, 264
BseBI CCWGG 1 cut(s) 597
BseDI CCNNGG 1 cut(s) 278
BseGI GGATG 5 cut(s) 172, 423, 610, 668, 704
BseJI GATNNNNATC 2 cut(s) 138, 264
BseMI GCAATG 1 cut(s) 837
BseRI GAGGAG 1 cut(s) 700
BseXI GCAGC 1 cut(s) 807
BshFI GGCC 3 cut(s) 96, 442, 595
BshNI GGYRCC 1 cut(s) 619
BsiHKCI CYCGRG 1 cut(s) 761
BsiSI CCGG 3 cut(s) 159, 405, 786
BsmAI GTCTC 2 cut(s) 425, 910
BsnI GGCC 3 cut(s) 96, 442, 595
BsoBI CYCGRG 1 cut(s) 761
Bsp1407I TGTACA 1 cut(s) 585
BspACI CCGC 4 cut(s) 93, 299, 533, 1019
BspANI GGCC 3 cut(s) 96, 442, 595
BspHI TCATGA 3 cut(s) 136, 142, 513
BspLI GGNNCC 1 cut(s) 621
BspMAI CTGCAG 1 cut(s) 1048
BspPI GGATC 2 cut(s) 269, 518
BspT107I GGYRCC 1 cut(s) 619
BspTI CTTAAG 1 cut(s) 682
BsrDI GCAATG 1 cut(s) 837
BsrGI TGTACA 1 cut(s) 585
BssECI CCNNGG 1 cut(s) 278
BssT1I CCWWGG 1 cut(s) 278
Bst2UI CCWGG 1 cut(s) 597
Bst4CI ACNGT 1 cut(s) 821
BstAFI CTTAAG 1 cut(s) 682
BstAUI TGTACA 1 cut(s) 585
BstF5I GGATG 5 cut(s) 172, 423, 610, 668, 704
BstMAI GTCTC 2 cut(s) 425, 910
BstMWI GCNNNNNNNGC 4 cut(s) 299, 407, 795, 1025
BstNI CCWGG 1 cut(s) 597
BstSCI CCNGG 2 cut(s) 157, 595
BstSFI CTRYAG 1 cut(s) 1044
BstV1I GCAGC 1 cut(s) 807
BstV2I GAAGAC 2 cut(s) 81, 521
BsuI GTATCC 1 cut(s) 22
BsuRI GGCC 3 cut(s) 96, 442, 595
BtsCI GGATG 5 cut(s) 172, 423, 610, 668, 704
CciI TCATGA 3 cut(s) 136, 142, 513
Csp6I GTAC 6 cut(s) 322, 501, 553, 586, 620, 1094
CviAII CATG 9 cut(s) 37, 107, 137, 143, 148, 154, 514, 539, 1036
CviQI GTAC 6 cut(s) 322, 501, 553, 586, 620, 1094
EaeI YGGCCR 1 cut(s) 94
Eco130I CCWWGG 1 cut(s) 278
Eco57I CTGAAG 1 cut(s) 369
Eco88I CYCGRG 1 cut(s) 761
EcoRII CCWGG 1 cut(s) 595
EcoT14I CCWWGG 1 cut(s) 278
ErhI CCWWGG 1 cut(s) 278
FaeI CATG 9 cut(s) 40, 110, 140, 146, 151, 157, 517, 542, 1039
FatI CATG 9 cut(s) 36, 106, 136, 142, 147, 153, 513, 538, 1035
FbaI TGATCA 4 cut(s) 82, 133, 139, 420
Fnu4HI GCNGC 4 cut(s) 94, 300, 796, 1020
FokI GGATG 5 cut(s) 159, 430, 597, 655, 691
Fsp4HI GCNGC 4 cut(s) 94, 300, 796, 1020
FspBI CTAG 4 cut(s) 464, 504, 845, 965
GluI GCNGC 4 cut(s) 94, 300, 796, 1020
HaeIII GGCC 3 cut(s) 96, 442, 595
HapII CCGG 3 cut(s) 159, 405, 786
Hin1II CATG 9 cut(s) 40, 110, 140, 146, 151, 157, 517, 542, 1039
HincII GTYRAC 3 cut(s) 52, 949, 970
HindII GTYRAC 3 cut(s) 52, 949, 970
HinfI GANTC 5 cut(s) 103, 168, 1039, 1054, 1084
HpaI GTTAAC 1 cut(s) 970
HpaII CCGG 3 cut(s) 159, 405, 786
Hpy166II GTNNAC 4 cut(s) 52, 586, 949, 970
Hpy188I TCNGA 7 cut(s) 345, 425, 430, 449, 724, 829, 985
Hpy188III TCNNGA 7 cut(s) 137, 143, 387, 514, 761, 763, 923
Hpy8I GTNNAC 4 cut(s) 52, 586, 949, 970
Hpy99I CGWCG 1 cut(s) 794
HpyAV CCTTC 2 cut(s) 215, 215
HpyCH4III ACNGT 1 cut(s) 821
HpyCH4IV ACGT 2 cut(s) 625, 831
HpyCH4V TGCA 1 cut(s) 1046
HpyF10VI GCNNNNNNNGC 4 cut(s) 299, 407, 795, 1025
HpySE526I ACGT 2 cut(s) 625, 831
Hsp92II CATG 9 cut(s) 40, 110, 140, 146, 151, 157, 517, 542, 1039
KpnI GGTACC 1 cut(s) 623
Ksp22I TGATCA 4 cut(s) 82, 133, 139, 420
KspAI GTTAAC 1 cut(s) 970
LmnI GCTCC 2 cut(s) 407, 989
Lsp1109I GCAGC 1 cut(s) 807
LweI GCATC 1 cut(s) 36
MaeI CTAG 4 cut(s) 464, 504, 845, 965
MaeII ACGT 2 cut(s) 625, 831
MaeIII GTNAC 4 cut(s) 41, 376, 821, 832
MboII GAAGA 7 cut(s) 86, 131, 194, 197, 521, 617, 947
MfeI CAATTG 1 cut(s) 6
MluCI AATT 1 cut(s) 6
MlyI GAGTC 1 cut(s) 112
MmeI TCCRAC 2 cut(s) 488, 747
MnlI CCTC 6 cut(s) 25, 83, 266, 718, 721, 1017
MseI TTAA 6 cut(s) 234, 452, 683, 735, 969, 1134
MslI CAYNNNNRTG 3 cut(s) 35, 141, 152
MspCI CTTAAG 1 cut(s) 682
MspI CCGG 3 cut(s) 159, 405, 786
MspR9I CCNGG 2 cut(s) 159, 597
MunI CAATTG 1 cut(s) 6
MvaI CCWGG 1 cut(s) 597
MwoI GCNNNNNNNGC 4 cut(s) 299, 407, 795, 1025
NciI CCSGG 1 cut(s) 159
NlaIII CATG 9 cut(s) 40, 110, 140, 146, 151, 157, 517, 542, 1039
NlaIV GGNNCC 1 cut(s) 621
NmeAIII GCCGAG 2 cut(s) 418, 605
PaeR7I CTCGAG 1 cut(s) 761
PagI TCATGA 3 cut(s) 136, 142, 513
PfeI GAWTC 4 cut(s) 168, 1039, 1054, 1084
PflFI GACNNNGTC 1 cut(s) 149
PkrI GCNGC 4 cut(s) 95, 301, 797, 1021
PleI GAGTC 1 cut(s) 111
PpsI GAGTC 1 cut(s) 111
Psp6I CCWGG 1 cut(s) 595
PspGI CCWGG 1 cut(s) 595
PspN4I GGNNCC 1 cut(s) 621
PstI CTGCAG 1 cut(s) 1048
PsyI GACNNNGTC 1 cut(s) 149
RsaI GTAC 6 cut(s) 323, 502, 554, 587, 621, 1095
RsaNI GTAC 6 cut(s) 322, 501, 553, 586, 620, 1094
RseI CAYNNNNRTG 3 cut(s) 35, 141, 152
SaqAI TTAA 6 cut(s) 234, 452, 683, 735, 969, 1134
SatI GCNGC 4 cut(s) 94, 300, 796, 1020
SbfI CCTGCAGG 1 cut(s) 1048
SchI GAGTC 1 cut(s) 112
ScrFI CCNGG 2 cut(s) 159, 597
SdaI CCTGCAGG 1 cut(s) 1048
SetI ASST 9 cut(s) 70, 130, 207, 412, 628, 735, 771, 834, 1030
SfaNI GCATC 1 cut(s) 36
SfcI CTRYAG 1 cut(s) 1044
Sfr274I CTCGAG 1 cut(s) 761
SlaI CTCGAG 1 cut(s) 761
SmiMI CAYNNNNRTG 3 cut(s) 35, 141, 152
SmlI CTYRAG 3 cut(s) 682, 761, 1014
SmoI CTYRAG 3 cut(s) 682, 761, 1014
SpeI ACTAGT 2 cut(s) 503, 964
Sse8387I CCTGCAGG 1 cut(s) 1048
Sse9I AATT 1 cut(s) 6
SsiI CCGC 4 cut(s) 93, 299, 533, 1019
SspI AATATT 2 cut(s) 782, 1126
SspMI CTAG 4 cut(s) 464, 504, 845, 965
StyD4I CCNGG 2 cut(s) 157, 595
StyI CCWWGG 1 cut(s) 278
TaaI ACNGT 1 cut(s) 821
TaiI ACGT 2 cut(s) 628, 834
TaqI TCGA 3 cut(s) 258, 662, 762
TaqII GACCGA 1 cut(s) 382
TasI AATT 1 cut(s) 6
TatI WGTACW 1 cut(s) 585
TauI GCSGC 3 cut(s) 96, 302, 1022
TfiI GAWTC 4 cut(s) 168, 1039, 1054, 1084
Tru1I TTAA 6 cut(s) 234, 452, 683, 735, 969, 1134
Tru9I TTAA 6 cut(s) 234, 452, 683, 735, 969, 1134
TseI GCWGC 1 cut(s) 795
TspDTI ATGAA 7 cut(s) 132, 138, 530, 555, 693, 948, 1046
Tth111I GACNNNGTC 1 cut(s) 149
Vha464I CTTAAG 1 cut(s) 682
XcmI CCANNNNNNNNNTGG 1 cut(s) 983
XhoI CTCGAG 1 cut(s) 761
XspI CTAG 4 cut(s) 464, 504, 845, 965
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.