MD15G1444700.v1.1
NAC Family

NAC domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0010263
Physical Location & Seq
Reverse (-)
54509755 .. 54511897
2143 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1444700.v1.1.491

Sequence Viewer

Length: 1446 bp
ATGGCAATTGCAGCAACAAGAACAACCACCTCATCATCATCAACAATGAGCCACGACGACTCCAACAAGGCCGATCATGATCATGATCATGAGCATGATGAGCATGTTGATGAGCATGAGCACGACATGGTGATGCCGGGTTTTCGCTTCCACCCTACTGAGGAGGAACTTGTAGAGTTCTACCTACGCCGTAAGGTTGAGGGCAAGCGCTTCAATGTCGAACTTATTACTTTTCTCGATCTTTATCGCTATGACCCTTGGGAACTTCCAGCTATGGCAGCAATCGGGGAGAAGGAATGGTTCTTTTATGTACCAAGAGACCGCAAGTACCGCAACGGCGATAGGCCAAACCGGGTAACTACTTCTGGGTATTGGAAGGCAACTGGAGCCGACCGTATGATCCGAAGCGAAAACTTCCGCTCAATCGGCCTCAAGAAAACCCTAGTCTTTTACTCTGGGAAAGCTCCCAAGGGTCTTCGAACTAGTTGGATCATGAACGAATATCGCTTGCCTCATCATGAAACTGAACGATATCAAAAGGGAGAAATATCACTATGCCGAGTATACAAGAGAGCAGGAGTTGAAGACCACCCATCACTCCCTCGTTGTCTCCCGGCATCCAGGGCATCCACATCTAGATCATCAGTACTAGTAGATCAATCGACGACCATGTCAGACAATAAAAAGCAGCACACCAATAATACCAACACAATTGATACTGTGATGGAAAAAATCCAAGCTTTCAATGAAGGCCAATCGCAGCAAATTCATCATGATCATGAACATCAGATGGATCAAAAGATTAGCATCGAAACTGAAGGTAGTAGCGGAAATTCTGATGTGACAACAGTTTTGGGACTTTCGAAGTATAAGGTGTACCATCACGGCCACCACCAGCCCCACAACATTGAGAGTAGTACTAGTACCGCTGCCGCACTTGGACAACTGGGTACTCCAACTCATCATGTGGATCAGGAAGAAGGGATGCCTACTTTCTTGCACCACCTAACAAACTCAAGGCAAATTGCTGCTAATTGCTATTCGGGGATCTCAAGTACTGGCAATTCATGTGCAGCGCTCTTCACCGGTGGTTCTTCTTCTGTTTCATCTTCCAATATGAATGCAATTGATGATCTTCATAGACTTTTGAATTACCAACCTGTACACGTACAGCAGCAGGCCACCTCCAATCATTACCAACAGCAACAATATGTACACCATCATCTAGTACAATACTATGATCATCACCATCATCATCCTCCCCAACCCAACAACTTTTTCTCCACAAATTTACCTGCAGCAGCGGCAGCACTATCAACTATACAGCCACAGCCGCAGCAGCAGCTCCCCCCCAACGCACTTCCAACTGCTTTCTCTGACCGCCTATGGGACTGGAATCCAATATCAGAGCCAAACCGGCCGGACTACAACAATCCGTTCAAGTAA

Protein Analysis

482

Amino Acids

54.89

Weight (kDa)

6.42

Isoelectric Point (pI)

47.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM PF02365 46 - 170 1.3e-44 No apical meristem (NAM) protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 670
Acc36I ACCTGC 1 cut(s) 1303
AccBSI CCGCTC 1 cut(s) 420
AccI GTMKAC 1 cut(s) 564
AciI CCGC 9 cut(s) 322, 331, 418, 828, 927, 933, 1304, 1334, 1381
AclWI GGATC 5 cut(s) 394, 497, 801, 978, 1055
AcoI YGGCCR 2 cut(s) 886, 1418
AcsI RAATTY 3 cut(s) 765, 832, 1288
AcuI CTGAAG 1 cut(s) 837
AfeI AGCGCT 2 cut(s) 209, 1077
AfiI CCNNNNNNNGG 2 cut(s) 160, 1387
AflIII ACRYGT 1 cut(s) 1165
AgeI ACCGGT 1 cut(s) 1085
AgsI TTSAA 5 cut(s) 214, 584, 745, 1150, 1441
AhlI ACTAGT 3 cut(s) 482, 649, 920
AjnI CCWGG 1 cut(s) 620
AjuI GAANNNNNNNTTGG 2 cut(s) 1262, 1294
AluBI AGCT 4 cut(s) 272, 464, 740, 1345
AluI AGCT 4 cut(s) 272, 464, 740, 1345
Alw21I GWGCWC 1 cut(s) 123
Alw26I GTCTC 2 cut(s) 312, 614
AlwI GGATC 5 cut(s) 394, 497, 801, 978, 1055
Aor51HI AGCGCT 2 cut(s) 209, 1077
AoxI GGCC 7 cut(s) 69, 344, 427, 751, 886, 1179, 1418
ApoI RAATTY 3 cut(s) 765, 832, 1288
ArsI GACNNNNNNTTYG 2 cut(s) 835, 867
AsiGI ACCGGT 1 cut(s) 1085
AspLEI GCGC 2 cut(s) 210, 1078
AsuC2I CCSGG 3 cut(s) 138, 353, 614
AsuHPI GGTGA 3 cut(s) 142, 1075, 1238
AsuII TTCGAA 2 cut(s) 478, 863
BbsI GAAGAC 2 cut(s) 467, 591
Bbv12I GWGCWC 1 cut(s) 123
BccI CCATC 6 cut(s) 601, 718, 784, 888, 1227, 1257
BceAI ACGGC 3 cut(s) 174, 352, 901
BciT130I CCWGG 1 cut(s) 622
BclI TGATCA 4 cut(s) 79, 85, 775, 1240
BcnI CCSGG 3 cut(s) 138, 353, 614
BcoDI GTCTC 2 cut(s) 312, 614
BcuI ACTAGT 3 cut(s) 482, 649, 920
BfaI CTAG 6 cut(s) 443, 483, 636, 650, 921, 1226
BfmI CTRYAG 1 cut(s) 1296
BfoI RGCGCY 2 cut(s) 211, 1079
BfuAI ACCTGC 1 cut(s) 1303
BglI GCCNNNNNGGC 1 cut(s) 1417
BmcAI AGTACT 3 cut(s) 648, 919, 1057
Bme1390I CCNGG 4 cut(s) 138, 353, 614, 622
BmiI GGNNCC 1 cut(s) 388
BmrFI CCNGG 4 cut(s) 138, 353, 614, 622
BmrI ACTGGG 1 cut(s) 956
BmsI GCATC 5 cut(s) 123, 626, 635, 816, 975
BmuI ACTGGG 1 cut(s) 956
BpiI GAAGAC 2 cut(s) 467, 591
BpmI CTGGAG 1 cut(s) 405
Bpu14I TTCGAA 2 cut(s) 478, 863
BpuEI CTTGAG 3 cut(s) 416, 1000, 1036
BpuMI CCSGG 3 cut(s) 138, 353, 614
BsaAI YACGTR 1 cut(s) 1168
BsaBI GATNNNNATC 4 cut(s) 78, 84, 243, 806
BsaI GGTCTC 1 cut(s) 312
BsaJI CCNNGG 3 cut(s) 257, 468, 621
BsaWI WCCGGW 1 cut(s) 1085
BsaXI ACNNNNNCTCC 4 cut(s) 44, 74, 1265, 1295
Bsc4I CCNNNNNNNGG 2 cut(s) 160, 1387
Bse118I RCCGGY 2 cut(s) 1085, 1416
Bse1I ACTGG 4 cut(s) 388, 951, 1063, 1397
Bse8I GATNNNNATC 4 cut(s) 78, 84, 243, 806
BseBI CCWGG 1 cut(s) 622
BseDI CCNNGG 3 cut(s) 257, 468, 621
BseGI GGATG 4 cut(s) 617, 626, 990, 1255
BseJI GATNNNNATC 4 cut(s) 78, 84, 243, 806
BseLI CCNNNNNNNGG 2 cut(s) 160, 1387
BseMII CTCAG 1 cut(s) 150
BseNI ACTGG 4 cut(s) 388, 951, 1063, 1397
BseRI GAGGAG 1 cut(s) 176
BseX3I CGGCCG 1 cut(s) 1418
BsgI GTGCAG 1 cut(s) 1092
Bsh1285I CGRYCG 2 cut(s) 394, 1421
BshFI GGCC 7 cut(s) 71, 346, 429, 753, 888, 1181, 1420
BshTI ACCGGT 1 cut(s) 1085
BsiEI CGRYCG 2 cut(s) 394, 1421
BsiHKAI GWGCWC 1 cut(s) 123
BsiSI CCGG 6 cut(s) 137, 352, 614, 1086, 1417, 1421
BslFI GGGAC 2 cut(s) 870, 1403
BslI CCNNNNNNNGG 2 cut(s) 160, 1387
BsmAI GTCTC 2 cut(s) 312, 614
BsmFI GGGAC 2 cut(s) 870, 1403
BsmI GAATGC 1 cut(s) 1126
BsnI GGCC 7 cut(s) 71, 346, 429, 753, 888, 1181, 1420
Bso31I GGTCTC 1 cut(s) 312
Bsp119I TTCGAA 2 cut(s) 478, 863
Bsp1286I GDGCHC 1 cut(s) 123
Bsp1407I TGTACA 2 cut(s) 1162, 1213
BspACI CCGC 9 cut(s) 322, 331, 418, 828, 927, 933, 1304, 1334, 1381
BspANI GGCC 7 cut(s) 71, 346, 429, 753, 888, 1181, 1420
BspCNI CTCAG 1 cut(s) 151
BspHI TCATGA 7 cut(s) 76, 82, 88, 492, 517, 772, 778
BspLI GGNNCC 1 cut(s) 388
BspMAI CTGCAG 1 cut(s) 1300
BspMI ACCTGC 1 cut(s) 1303
BspPI GGATC 5 cut(s) 394, 497, 801, 978, 1055
BspQI GCTCTTC 1 cut(s) 1085
BspT104I TTCGAA 2 cut(s) 478, 863
BspTNI GGTCTC 1 cut(s) 312
BsrBI CCGCTC 1 cut(s) 420
BsrFI RCCGGY 2 cut(s) 1085, 1416
BsrGI TGTACA 2 cut(s) 1162, 1213
BsrI ACTGG 4 cut(s) 388, 951, 1063, 1397
BssAI RCCGGY 2 cut(s) 1085, 1416
BssECI CCNNGG 3 cut(s) 257, 468, 621
BssNAI GTATAC 1 cut(s) 565
BssT1I CCWWGG 2 cut(s) 257, 468
Bst1107I GTATAC 1 cut(s) 565
Bst2UI CCWGG 1 cut(s) 622
Bst4CI ACNGT 3 cut(s) 395, 721, 850
Bst6I CTCTTC 1 cut(s) 1085
BstAUI TGTACA 2 cut(s) 1162, 1213
BstBAI YACGTR 1 cut(s) 1168
BstBI TTCGAA 2 cut(s) 478, 863
BstC8I GCNNGC 3 cut(s) 206, 509, 1179
BstDEI CTNAG 1 cut(s) 159
BstF5I GGATG 4 cut(s) 617, 626, 990, 1255
BstH2I RGCGCY 2 cut(s) 211, 1079
BstHHI GCGC 2 cut(s) 210, 1078
BstMAI GTCTC 2 cut(s) 312, 614
BstMCI CGRYCG 2 cut(s) 394, 1421
BstNI CCWGG 1 cut(s) 622
BstNSI RCATGY 1 cut(s) 107
BstSCI CCNGG 4 cut(s) 136, 351, 612, 620
BstSFI CTRYAG 1 cut(s) 1296
BstV2I GAAGAC 2 cut(s) 467, 591
BstX2I RGATCY 1 cut(s) 1047
BstYI RGATCY 1 cut(s) 1047
BstZ17I GTATAC 1 cut(s) 565
BstZI CGGCCG 1 cut(s) 1418
BsuRI GGCC 7 cut(s) 71, 346, 429, 753, 888, 1181, 1420
BtsCI GGATG 4 cut(s) 617, 626, 990, 1255
BveI ACCTGC 1 cut(s) 1303
Cac8I GCNNGC 3 cut(s) 206, 509, 1179
CciI TCATGA 7 cut(s) 76, 82, 88, 492, 517, 772, 778
CfoI GCGC 2 cut(s) 210, 1078
Cfr10I RCCGGY 2 cut(s) 1085, 1416
CspAI ACCGGT 1 cut(s) 1085
DdeI CTNAG 1 cut(s) 159
DrdI GACNNNNNNGTC 1 cut(s) 670
DseDI GACNNNNNNGTC 1 cut(s) 670
EaeI YGGCCR 2 cut(s) 886, 1418
EagI CGGCCG 1 cut(s) 1418
Eam1104I CTCTTC 1 cut(s) 1085
EarI CTCTTC 1 cut(s) 1085
EclXI CGGCCG 1 cut(s) 1418
Eco130I CCWWGG 2 cut(s) 257, 468
Eco31I GGTCTC 1 cut(s) 312
Eco32I GATATC 1 cut(s) 533
Eco47III AGCGCT 2 cut(s) 209, 1077
Eco52I CGGCCG 1 cut(s) 1418
Eco57I CTGAAG 1 cut(s) 837
EcoRII CCWGG 1 cut(s) 620
EcoRV GATATC 1 cut(s) 533
EcoT14I CCWWGG 2 cut(s) 257, 468
ErhI CCWWGG 2 cut(s) 257, 468
FaqI GGGAC 2 cut(s) 870, 1403
FbaI TGATCA 4 cut(s) 79, 85, 775, 1240
FblI GTMKAC 1 cut(s) 564
FokI GGATG 4 cut(s) 604, 613, 997, 1242
FspBI CTAG 6 cut(s) 443, 483, 636, 650, 921, 1226
GlaI GCGC 2 cut(s) 209, 1077
GsuI CTGGAG 1 cut(s) 405
HaeII RGCGCY 2 cut(s) 211, 1079
HaeIII GGCC 7 cut(s) 71, 346, 429, 753, 888, 1181, 1420
HapII CCGG 6 cut(s) 137, 352, 614, 1086, 1417, 1421
HhaI GCGC 2 cut(s) 210, 1078
Hin6I GCGC 2 cut(s) 208, 1076
HinP1I GCGC 2 cut(s) 208, 1076
HindIII AAGCTT 1 cut(s) 738
HinfI GANTC 2 cut(s) 59, 1396
HpaII CCGG 6 cut(s) 137, 352, 614, 1086, 1417, 1421
HphI GGTGA 3 cut(s) 142, 1075, 1238
Hpy166II GTNNAC 4 cut(s) 565, 877, 1165, 1216
Hpy188I TCNGA 6 cut(s) 404, 676, 789, 838, 1378, 1408
Hpy8I GTNNAC 4 cut(s) 565, 877, 1165, 1216
Hpy99I CGWCG 2 cut(s) 59, 667
HpyAV CCTTC 5 cut(s) 286, 370, 743, 812, 974
HpyCH4III ACNGT 3 cut(s) 395, 721, 850
HpyCH4IV ACGT 1 cut(s) 1167
HpyCH4V TGCA 5 cut(s) 11, 1000, 1073, 1124, 1298
HpyF3I CTNAG 1 cut(s) 159
HpySE526I ACGT 1 cut(s) 1167
HspAI GCGC 2 cut(s) 208, 1076
Ksp22I TGATCA 4 cut(s) 79, 85, 775, 1240
LguI GCTCTTC 1 cut(s) 1085
LmnI GCTCC 3 cut(s) 386, 469, 1350
LweI GCATC 5 cut(s) 123, 626, 635, 816, 975
MaeI CTAG 6 cut(s) 443, 483, 636, 650, 921, 1226
MaeII ACGT 1 cut(s) 1167
MaeIII GTNAC 2 cut(s) 355, 841
MbiI CCGCTC 1 cut(s) 420
MboII GAAGA 8 cut(s) 467, 596, 989, 1072, 1086, 1089, 1101, 1127
MfeI CAATTG 3 cut(s) 6, 711, 1125
MflI RGATCY 1 cut(s) 1047
MhlI GDGCHC 1 cut(s) 123
MlyI GAGTC 1 cut(s) 53
MmeI TCCRAC 4 cut(s) 87, 467, 980, 1388
MnlI CCTC 9 cut(s) 40, 154, 157, 193, 440, 522, 612, 1195, 1269
MslI CAYNNNNRTG 6 cut(s) 81, 87, 93, 108, 131, 777
MspA1I CMGCKG 2 cut(s) 929, 1304
MspI CCGG 6 cut(s) 137, 352, 614, 1086, 1417, 1421
MspR9I CCNGG 4 cut(s) 138, 353, 614, 622
MunI CAATTG 3 cut(s) 6, 711, 1125
Mva1269I GAATGC 1 cut(s) 1126
MvaI CCWGG 1 cut(s) 622
NciI CCSGG 3 cut(s) 138, 353, 614
NlaIV GGNNCC 1 cut(s) 388
NmeAIII GCCGAG 1 cut(s) 584
NmuCI GTSAC 1 cut(s) 841
NspI RCATGY 1 cut(s) 107
NspV TTCGAA 2 cut(s) 478, 863
PagI TCATGA 7 cut(s) 76, 82, 88, 492, 517, 772, 778
PciSI GCTCTTC 1 cut(s) 1085
PctI GAATGC 1 cut(s) 1126
PfeI GAWTC 1 cut(s) 1396
PflFI GACNNNGTC 1 cut(s) 670
PinAI ACCGGT 1 cut(s) 1085
PleI GAGTC 1 cut(s) 53
PpsI GAGTC 1 cut(s) 53
Ppu21I YACGTR 1 cut(s) 1168
Psp6I CCWGG 1 cut(s) 620
PspGI CCWGG 1 cut(s) 620
PspN4I GGNNCC 1 cut(s) 388
PstI CTGCAG 1 cut(s) 1300
PsuI RGATCY 1 cut(s) 1047
PsyI GACNNNGTC 1 cut(s) 670
RseI CAYNNNNRTG 6 cut(s) 81, 87, 93, 108, 131, 777
SapI GCTCTTC 1 cut(s) 1085
ScaI AGTACT 3 cut(s) 648, 919, 1057
SchI GAGTC 1 cut(s) 53
ScrFI CCNGG 4 cut(s) 138, 353, 614, 622
SduI GDGCHC 1 cut(s) 123
SfaNI GCATC 5 cut(s) 123, 626, 635, 816, 975
SfcI CTRYAG 1 cut(s) 1296
SfuI TTCGAA 2 cut(s) 478, 863
SgrAI CRCCGGYG 1 cut(s) 1085
SmiMI CAYNNNNRTG 6 cut(s) 81, 87, 93, 108, 131, 777
SmlI CTYRAG 3 cut(s) 431, 1015, 1051
SmoI CTYRAG 3 cut(s) 431, 1015, 1051
SpeI ACTAGT 3 cut(s) 482, 649, 920
SsiI CCGC 9 cut(s) 322, 331, 418, 828, 927, 933, 1304, 1334, 1381
SspMI CTAG 6 cut(s) 443, 483, 636, 650, 921, 1226
StyD4I CCNGG 4 cut(s) 136, 351, 612, 620
StyI CCWWGG 2 cut(s) 257, 468
TaaI ACNGT 3 cut(s) 395, 721, 850
TaiI ACGT 1 cut(s) 1170
TaqI TCGA 6 cut(s) 219, 237, 478, 662, 810, 863
TatI WGTACW 6 cut(s) 646, 917, 1055, 1162, 1213, 1228
TauI GCSGC 3 cut(s) 935, 1307, 1336
TfiI GAWTC 1 cut(s) 1396
TseFI GTSAC 1 cut(s) 841
Tsp45I GTSAC 1 cut(s) 841
TspDTI ATGAA 9 cut(s) 509, 534, 758, 762, 795, 1056, 1095, 1127, 1133
TspGWI ACGGA 1 cut(s) 1425
Tth111I GACNNNGTC 1 cut(s) 670
XapI RAATTY 3 cut(s) 765, 832, 1288
XbaI TCTAGA 1 cut(s) 635
XceI RCATGY 1 cut(s) 107
XmiI GTMKAC 1 cut(s) 564
XspI CTAG 6 cut(s) 443, 483, 636, 650, 921, 1226
ZrmI AGTACT 3 cut(s) 648, 919, 1057
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.