MD08G1250000.v1.1
NAC Family

NAC domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0010263
Physical Location & Seq
Reverse (-)
31430542 .. 31432937
2396 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1250000.v1.1.491

Sequence Viewer

Length: 1455 bp
ATGCCCAATCTAGTTGCAGAAACAACAACAACCTCATCATCATCAACAATGAGCCACGACGACTCCAACAAGGCCGACCATGATCATGAGCATGAGCATGATGAGTATGTTGATGAGCATGAGCACGACATGGTGATGCCGGGCTTTCGCTTCCACCCAACTGAGGAGGAACTTGTAGAGTTCTACCTTCGCCGTAAGGTTGAGGGCAAGCGCTTCAATGTTGAACTCATTACTTTTCTCGATCTTTATCGCTATGACCCTTGGGAACTTCCAGCTATGGCAGCAATTGGGGAGAAGGAATGGTTCTTTTATGTGCCAAGAGACCGCAAGTACCGCAACGGCGATAGGCCAAACCGGGTAACTACTTCTGGGTATTGGAAGGCAACTGGAGCCGACCGTATGATCCGAAGCGAAAACTTCCGCTCAATCGGCCTCAAGAAAACCCTAGTCTTTTACTCCGGGAAAGCTCCCAAAGGCATTCGAACTAGTTGGATCATGAACGAATATCGCTTGCCGCATCATGAAACTGAACGATATCAAAAGGGAGAAATATCACTATGCCGAGTATACAAGAGAGCCGGAGTTGAAGACCACCCATCACTCCCTCGTTGTCTCCCGGCATCCAGGGCATCCACATCTAGATCATCAGTACTAGTGGCTCAATCGACGACCAGGTCAGACAATAAAAAGCAGCATACCAATAATATCAACACAGTTGATAGTGTGATTGAAAAACTCCAAGCTTTTAATGAAGGCCAATCGCAGCAGATTCATCATGATCATGAACATCAGATGGATCAAAAGATGAACATCGAAACTGAAGGTAGCAGCAGAAATTCTGATGTGACAACAGTTTTGCGGCTTTCCAAGCATAAAGCGTACCCTCAGGGCCACCACCAGCACCACAACATTGAGAGTAGTACCGCTGCCGCACCTGGACAACCGGGTGCTCCAACTCATCCTGTGGATCAGGAAGAAGGGATGGCTGCTTTCTCGCACCCCTCAGCAAACTCAAAGCAATCTGGTGCTAATTGCTCTTCGGTGATCTCGAGTACTGGCAATTCATGTGCAGCACTCTTCACGAGTGGTTCTTCTTCTGTTTTGTCTTCCAATATGAATGCGATTGATGATCTTCATAGACTTTTGAATTACCAACCTGTACACGTACAGCAGCAGGCTGCTTCCAATCATCACCAACATCAACAATATGTACATCATCATCTAGTGCAATACTATGATCATCATCATCATCATCCCCAACCCAGCAACCTTTTCTCAACAAATTTCCCTTCAGCGGCGGCAGCACTATCAACTATACAGCCACAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCTCCCCTCTAACGCACTTCCGACTGCTTTCTCTGACCGCCTATGGGACTGGAATCCAATATCAGAGCCAACCCGGCCGGACTACAACAATCTGTTCAAGTAA

Protein Analysis

485

Amino Acids

55.19

Weight (kDa)

6.53

Isoelectric Point (pI)

56.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM PF02365 47 - 171 6.7e-45 No apical meristem (NAM) protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 673
AccBSI CCGCTC 1 cut(s) 423
AccI GTMKAC 1 cut(s) 567
AclWI GGATC 4 cut(s) 397, 500, 804, 975
AcoI YGGCCR 1 cut(s) 1427
AcsI RAATTY 2 cut(s) 835, 1282
AcuI CTGAAG 2 cut(s) 840, 1275
AfaI GTAC 8 cut(s) 332, 651, 881, 922, 1054, 1161, 1167, 1212
AfeI AGCGCT 1 cut(s) 212
AfiI CCNNNNNNNGG 3 cut(s) 163, 1294, 1396
AflIII ACRYGT 1 cut(s) 1162
AgsI TTSAA 6 cut(s) 217, 224, 587, 731, 1147, 1450
AhlI ACTAGT 2 cut(s) 485, 652
AjnI CCWGG 3 cut(s) 623, 671, 934
AluBI AGCT 4 cut(s) 275, 467, 743, 1354
AluI AGCT 4 cut(s) 275, 467, 743, 1354
Alw21I GWGCWC 2 cut(s) 126, 952
Alw26I GTCTC 2 cut(s) 315, 617
AlwI GGATC 4 cut(s) 397, 500, 804, 975
Ama87I CYCGRG 1 cut(s) 1048
Aor51HI AGCGCT 1 cut(s) 212
AoxI GGCC 6 cut(s) 72, 347, 430, 754, 889, 1427
ApoI RAATTY 2 cut(s) 835, 1282
ArsI GACNNNNNNTTYG 2 cut(s) 838, 870
AspLEI GCGC 1 cut(s) 213
AspS9I GGNCC 1 cut(s) 889
AsuC2I CCSGG 6 cut(s) 141, 356, 460, 617, 945, 1426
AsuHPI GGTGA 3 cut(s) 145, 1054, 1184
AsuII TTCGAA 1 cut(s) 481
AvaI CYCGRG 1 cut(s) 1048
AxyI CCTNAGG 1 cut(s) 885
BauI CACGAG 1 cut(s) 1081
BbsI GAAGAC 2 cut(s) 594, 1098
Bbv12I GWGCWC 2 cut(s) 126, 952
BbvCI CCTCAGC 1 cut(s) 1003
BccI CCATC 3 cut(s) 604, 787, 976
BceAI ACGGC 2 cut(s) 177, 355
BciT130I CCWGG 3 cut(s) 625, 673, 936
BclI TGATCA 3 cut(s) 82, 778, 1237
BcnI CCSGG 6 cut(s) 141, 356, 460, 617, 945, 1426
BcoDI GTCTC 2 cut(s) 315, 617
BcuI ACTAGT 2 cut(s) 485, 652
BfaI CTAG 6 cut(s) 11, 446, 486, 639, 653, 1223
BfoI RGCGCY 1 cut(s) 214
BglI GCCNNNNNGGC 1 cut(s) 1426
BmcAI AGTACT 2 cut(s) 651, 1054
Bme1390I CCNGG 9 cut(s) 141, 356, 460, 617, 625, 673, 936, 945, 1426
BmeT110I CYCGRG 1 cut(s) 1048
BmgT120I GGNCC 1 cut(s) 889
BmiI GGNNCC 1 cut(s) 391
BmrFI CCNGG 9 cut(s) 141, 356, 460, 617, 625, 673, 936, 945, 1426
BmsI GCATC 4 cut(s) 126, 526, 629, 638
BpiI GAAGAC 2 cut(s) 594, 1098
BpmI CTGGAG 1 cut(s) 408
Bpu10I CCTNAGC 1 cut(s) 1003
Bpu14I TTCGAA 1 cut(s) 481
BpuEI CTTGAG 1 cut(s) 419
BpuMI CCSGG 6 cut(s) 141, 356, 460, 617, 945, 1426
BsaAI YACGTR 1 cut(s) 1165
BsaBI GATNNNNATC 3 cut(s) 246, 809, 1242
BsaI GGTCTC 1 cut(s) 315
BsaJI CCNNGG 2 cut(s) 260, 624
BsaXI ACNNNNNCTCC 2 cut(s) 47, 77
Bsc4I CCNNNNNNNGG 3 cut(s) 163, 1294, 1396
Bse1I ACTGG 3 cut(s) 391, 1060, 1406
Bse21I CCTNAGG 1 cut(s) 885
Bse8I GATNNNNATC 3 cut(s) 246, 809, 1242
BseBI CCWGG 3 cut(s) 625, 673, 936
BseDI CCNNGG 2 cut(s) 260, 624
BseGI GGATG 5 cut(s) 620, 629, 958, 987, 1252
BseJI GATNNNNATC 3 cut(s) 246, 809, 1242
BseLI CCNNNNNNNGG 3 cut(s) 163, 1294, 1396
BseMII CTCAG 3 cut(s) 153, 899, 1017
BseNI ACTGG 3 cut(s) 391, 1060, 1406
BseRI GAGGAG 1 cut(s) 179
BseX3I CGGCCG 1 cut(s) 1427
BseYI CCCAGC 1 cut(s) 1262
BsgI GTGCAG 1 cut(s) 1089
Bsh1285I CGRYCG 2 cut(s) 397, 1430
BshFI GGCC 6 cut(s) 74, 349, 432, 756, 891, 1429
BsiEI CGRYCG 2 cut(s) 397, 1430
BsiHKAI GWGCWC 2 cut(s) 126, 952
BsiHKCI CYCGRG 1 cut(s) 1048
BsiSI CCGG 8 cut(s) 140, 355, 459, 579, 617, 944, 1426, 1430
BslFI GGGAC 1 cut(s) 1412
BslI CCNNNNNNNGG 3 cut(s) 163, 1294, 1396
BsmAI GTCTC 2 cut(s) 315, 617
BsmFI GGGAC 1 cut(s) 1412
BsmI GAATGC 2 cut(s) 477, 1123
BsnI GGCC 6 cut(s) 74, 349, 432, 756, 891, 1429
Bso31I GGTCTC 1 cut(s) 315
BsoBI CYCGRG 1 cut(s) 1048
Bsp119I TTCGAA 1 cut(s) 481
Bsp1286I GDGCHC 2 cut(s) 126, 952
Bsp1407I TGTACA 2 cut(s) 1159, 1210
BspANI GGCC 6 cut(s) 74, 349, 432, 756, 891, 1429
BspCNI CTCAG 3 cut(s) 154, 898, 1016
BspHI TCATGA 5 cut(s) 85, 495, 520, 775, 781
BspLI GGNNCC 1 cut(s) 391
BspPI GGATC 4 cut(s) 397, 500, 804, 975
BspQI GCTCTTC 1 cut(s) 1042
BspT104I TTCGAA 1 cut(s) 481
BspTNI GGTCTC 1 cut(s) 315
BsrBI CCGCTC 1 cut(s) 423
BsrGI TGTACA 2 cut(s) 1159, 1210
BsrI ACTGG 3 cut(s) 391, 1060, 1406
BssECI CCNNGG 2 cut(s) 260, 624
BssNAI GTATAC 1 cut(s) 568
BssSI CACGAG 1 cut(s) 1081
BssT1I CCWWGG 1 cut(s) 260
Bst1107I GTATAC 1 cut(s) 568
Bst2BI CACGAG 1 cut(s) 1081
Bst2UI CCWGG 3 cut(s) 625, 673, 936
Bst4CI ACNGT 3 cut(s) 398, 715, 853
Bst6I CTCTTC 2 cut(s) 1042, 1082
BstAUI TGTACA 2 cut(s) 1159, 1210
BstBAI YACGTR 1 cut(s) 1165
BstBI TTCGAA 1 cut(s) 481
BstC8I GCNNGC 3 cut(s) 209, 512, 1176
BstDEI CTNAG 3 cut(s) 162, 885, 1003
BstF5I GGATG 5 cut(s) 620, 629, 958, 987, 1252
BstH2I RGCGCY 1 cut(s) 214
BstHHI GCGC 1 cut(s) 213
BstMAI GTCTC 2 cut(s) 315, 617
BstMCI CGRYCG 2 cut(s) 397, 1430
BstNI CCWGG 3 cut(s) 625, 673, 936
BstSCI CCNGG 9 cut(s) 139, 354, 458, 615, 623, 671, 934, 943, 1424
BstV2I GAAGAC 2 cut(s) 594, 1098
BstZ17I GTATAC 1 cut(s) 568
BstZI CGGCCG 1 cut(s) 1427
Bsu36I CCTNAGG 1 cut(s) 885
BsuRI GGCC 6 cut(s) 74, 349, 432, 756, 891, 1429
BtsCI GGATG 5 cut(s) 620, 629, 958, 987, 1252
Cac8I GCNNGC 3 cut(s) 209, 512, 1176
CciI TCATGA 5 cut(s) 85, 495, 520, 775, 781
CfoI GCGC 1 cut(s) 213
Cfr13I GGNCC 1 cut(s) 889
CsiI ACCWGGT 1 cut(s) 671
Csp6I GTAC 8 cut(s) 331, 650, 880, 921, 1053, 1160, 1166, 1211
CviQI GTAC 8 cut(s) 331, 650, 880, 921, 1053, 1160, 1166, 1211
DdeI CTNAG 3 cut(s) 162, 885, 1003
DrdI GACNNNNNNGTC 1 cut(s) 673
DseDI GACNNNNNNGTC 1 cut(s) 673
EaeI YGGCCR 1 cut(s) 1427
EagI CGGCCG 1 cut(s) 1427
Eam1104I CTCTTC 2 cut(s) 1042, 1082
EarI CTCTTC 2 cut(s) 1042, 1082
EclXI CGGCCG 1 cut(s) 1427
Eco130I CCWWGG 1 cut(s) 260
Eco31I GGTCTC 1 cut(s) 315
Eco32I GATATC 1 cut(s) 536
Eco47III AGCGCT 1 cut(s) 212
Eco52I CGGCCG 1 cut(s) 1427
Eco57I CTGAAG 2 cut(s) 840, 1275
Eco81I CCTNAGG 1 cut(s) 885
Eco88I CYCGRG 1 cut(s) 1048
EcoRII CCWGG 3 cut(s) 623, 671, 934
EcoRV GATATC 1 cut(s) 536
EcoT14I CCWWGG 1 cut(s) 260
ErhI CCWWGG 1 cut(s) 260
FaqI GGGAC 1 cut(s) 1412
FbaI TGATCA 3 cut(s) 82, 778, 1237
FblI GTMKAC 1 cut(s) 567
FokI GGATG 5 cut(s) 607, 616, 945, 994, 1239
FspBI CTAG 6 cut(s) 11, 446, 486, 639, 653, 1223
GlaI GCGC 1 cut(s) 212
GsaI CCCAGC 1 cut(s) 1266
GsuI CTGGAG 1 cut(s) 408
HaeII RGCGCY 1 cut(s) 214
HaeIII GGCC 6 cut(s) 74, 349, 432, 756, 891, 1429
HapII CCGG 8 cut(s) 140, 355, 459, 579, 617, 944, 1426, 1430
HhaI GCGC 1 cut(s) 213
Hin6I GCGC 1 cut(s) 211
HinP1I GCGC 1 cut(s) 211
HindIII AAGCTT 1 cut(s) 741
HinfI GANTC 3 cut(s) 62, 769, 1405
HpaII CCGG 8 cut(s) 140, 355, 459, 579, 617, 944, 1426, 1430
HphI GGTGA 3 cut(s) 145, 1054, 1184
Hpy166II GTNNAC 2 cut(s) 568, 1162
Hpy188I TCNGA 7 cut(s) 407, 679, 792, 841, 1374, 1387, 1417
Hpy8I GTNNAC 2 cut(s) 568, 1162
Hpy99I CGWCG 2 cut(s) 62, 670
HpyAV CCTTC 7 cut(s) 197, 289, 373, 746, 815, 971, 1299
HpyCH4III ACNGT 3 cut(s) 398, 715, 853
HpyCH4IV ACGT 1 cut(s) 1164
HpyCH4V TGCA 3 cut(s) 17, 1070, 1228
HpyF3I CTNAG 3 cut(s) 162, 885, 1003
HpySE526I ACGT 1 cut(s) 1164
HspAI GCGC 1 cut(s) 211
Ksp22I TGATCA 3 cut(s) 82, 778, 1237
LguI GCTCTTC 1 cut(s) 1042
LmnI GCTCC 4 cut(s) 389, 472, 955, 1359
LweI GCATC 4 cut(s) 126, 526, 629, 638
MabI ACCWGGT 1 cut(s) 671
MaeI CTAG 6 cut(s) 11, 446, 486, 639, 653, 1223
MaeII ACGT 1 cut(s) 1164
MaeIII GTNAC 2 cut(s) 358, 844
MbiI CCGCTC 1 cut(s) 423
MboII GAAGA 8 cut(s) 599, 986, 1029, 1069, 1083, 1086, 1098, 1124
MfeI CAATTG 1 cut(s) 285
MhlI GDGCHC 2 cut(s) 126, 952
MluCI AATT 6 cut(s) 285, 835, 1030, 1060, 1147, 1282
MlyI GAGTC 1 cut(s) 56
MmeI TCCRAC 4 cut(s) 90, 470, 977, 1397
MnlI CCTC 9 cut(s) 43, 157, 160, 196, 443, 615, 894, 1012, 1369
MseI TTAA 1 cut(s) 747
MslI CAYNNNNRTG 5 cut(s) 84, 90, 96, 134, 780
MspA1I CMGCKG 2 cut(s) 926, 1295
MspI CCGG 8 cut(s) 140, 355, 459, 579, 617, 944, 1426, 1430
MspR9I CCNGG 9 cut(s) 141, 356, 460, 617, 625, 673, 936, 945, 1426
MunI CAATTG 1 cut(s) 285
Mva1269I GAATGC 2 cut(s) 477, 1123
MvaI CCWGG 3 cut(s) 625, 673, 936
NciI CCSGG 6 cut(s) 141, 356, 460, 617, 945, 1426
NlaIV GGNNCC 1 cut(s) 391
NmeAIII GCCGAG 1 cut(s) 587
NmuCI GTSAC 1 cut(s) 844
NspV TTCGAA 1 cut(s) 481
PaeR7I CTCGAG 1 cut(s) 1048
PagI TCATGA 5 cut(s) 85, 495, 520, 775, 781
PciSI GCTCTTC 1 cut(s) 1042
PcsI WCGNNNNNNNCGW 1 cut(s) 1046
PctI GAATGC 2 cut(s) 477, 1123
PfeI GAWTC 2 cut(s) 769, 1405
PflFI GACNNNGTC 1 cut(s) 673
PfoI TCCNGGA 1 cut(s) 458
PleI GAGTC 1 cut(s) 56
PpsI GAGTC 1 cut(s) 56
Ppu21I YACGTR 1 cut(s) 1165
Psp6I CCWGG 3 cut(s) 623, 671, 934
PspFI CCCAGC 1 cut(s) 1262
PspGI CCWGG 3 cut(s) 623, 671, 934
PspN4I GGNNCC 1 cut(s) 391
PspPI GGNCC 1 cut(s) 889
PsyI GACNNNGTC 1 cut(s) 673
RsaI GTAC 8 cut(s) 332, 651, 881, 922, 1054, 1161, 1167, 1212
RsaNI GTAC 8 cut(s) 331, 650, 880, 921, 1053, 1160, 1166, 1211
RseI CAYNNNNRTG 5 cut(s) 84, 90, 96, 134, 780
SapI GCTCTTC 1 cut(s) 1042
SaqAI TTAA 1 cut(s) 747
Sau96I GGNCC 1 cut(s) 889
ScaI AGTACT 2 cut(s) 651, 1054
SchI GAGTC 1 cut(s) 56
ScrFI CCNGG 9 cut(s) 141, 356, 460, 617, 625, 673, 936, 945, 1426
SduI GDGCHC 2 cut(s) 126, 952
SexAI ACCWGGT 1 cut(s) 671
SfaNI GCATC 4 cut(s) 126, 526, 629, 638
Sfr274I CTCGAG 1 cut(s) 1048
SfuI TTCGAA 1 cut(s) 481
SlaI CTCGAG 1 cut(s) 1048
SmiMI CAYNNNNRTG 5 cut(s) 84, 90, 96, 134, 780
SmlI CTYRAG 2 cut(s) 434, 1048
SmoI CTYRAG 2 cut(s) 434, 1048
SpeI ACTAGT 2 cut(s) 485, 652
Sse9I AATT 6 cut(s) 285, 835, 1030, 1060, 1147, 1282
SspMI CTAG 6 cut(s) 11, 446, 486, 639, 653, 1223
StyD4I CCNGG 9 cut(s) 139, 354, 458, 615, 623, 671, 934, 943, 1424
StyI CCWWGG 1 cut(s) 260
TaaI ACNGT 3 cut(s) 398, 715, 853
TaiI ACGT 1 cut(s) 1167
TaqI TCGA 5 cut(s) 240, 481, 665, 813, 1049
TasI AATT 6 cut(s) 285, 835, 1030, 1060, 1147, 1282
TatI WGTACW 4 cut(s) 649, 1052, 1159, 1210
TauI GCSGC 5 cut(s) 517, 862, 932, 1298, 1301
TfiI GAWTC 2 cut(s) 769, 1405
Tru1I TTAA 1 cut(s) 747
Tru9I TTAA 1 cut(s) 747
TseFI GTSAC 1 cut(s) 844
Tsp45I GTSAC 1 cut(s) 844
TspDTI ATGAA 9 cut(s) 512, 537, 761, 765, 798, 821, 1053, 1124, 1130
Tth111I GACNNNGTC 1 cut(s) 673
XapI RAATTY 2 cut(s) 835, 1282
XbaI TCTAGA 1 cut(s) 638
XhoI CTCGAG 1 cut(s) 1048
XmiI GTMKAC 1 cut(s) 567
XspI CTAG 6 cut(s) 11, 446, 486, 639, 653, 1223
ZrmI AGTACT 2 cut(s) 651, 1054
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.