CONTAINS InterPro DOMAIN s SPX, N-terminal (InterPro IPR004331), EXS, C-terminal (InterPro IPR004342)
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 453 |
| AccB1I | GGYRCC | 1 cut(s) | 34 |
| AccI | GTMKAC | 2 cut(s) | 2055, 2220 |
| AccII | CGCG | 3 cut(s) | 972, 2231, 2261 |
| AciI | CCGC | 9 cut(s) | 189, 237, 240, 614, 617, 639, 1453, 2207, 2347 |
| AclWI | GGATC | 4 cut(s) | 709, 849, 873, 2102 |
| AcoI | YGGCCR | 1 cut(s) | 618 |
| AcsI | RAATTY | 4 cut(s) | 101, 910, 926, 2362 |
| AcuI | CTGAAG | 2 cut(s) | 1931, 2353 |
| AcyI | GRCGYC | 1 cut(s) | 374 |
| AfaI | GTAC | 6 cut(s) | 395, 571, 1364, 1498, 2009, 2125 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 865, 1988 |
| AgeI | ACCGGT | 1 cut(s) | 1692 |
| AjiI | CACGTC | 1 cut(s) | 796 |
| AjnI | CCWGG | 1 cut(s) | 900 |
| AleI | CACNNNNGTG | 1 cut(s) | 249 |
| Alw21I | GWGCWC | 1 cut(s) | 670 |
| Alw26I | GTCTC | 7 cut(s) | 350, 392, 811, 1219, 1240, 1939, 1946 |
| AlwI | GGATC | 4 cut(s) | 709, 849, 873, 2102 |
| AoxI | GGCC | 5 cut(s) | 554, 618, 903, 1351, 1999 |
| ApeKI | GCWGC | 4 cut(s) | 626, 1739, 2111, 2266 |
| ApoI | RAATTY | 4 cut(s) | 101, 910, 926, 2362 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 349, 381 |
| AsiGI | ACCGGT | 1 cut(s) | 1692 |
| Asp700I | GAANNNNTTC | 3 cut(s) | 17, 1629, 2366 |
| AspLEI | GCGC | 2 cut(s) | 322, 974 |
| AspS9I | GGNCC | 1 cut(s) | 128 |
| AsuHPI | GGTGA | 3 cut(s) | 167, 198, 803 |
| AsuII | TTCGAA | 2 cut(s) | 1043, 1813 |
| AvaII | GGWCC | 1 cut(s) | 128 |
| BanI | GGYRCC | 1 cut(s) | 34 |
| BanII | GRGCYC | 1 cut(s) | 670 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 2204, 2236 |
| BbsI | GAAGAC | 1 cut(s) | 2147 |
| Bbv12I | GWGCWC | 1 cut(s) | 670 |
| BbvI | GCAGC | 4 cut(s) | 613, 1726, 2098, 2253 |
| BccI | CCATC | 3 cut(s) | 364, 542, 2308 |
| BceAI | ACGGC | 3 cut(s) | 250, 605, 2334 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 1793, 1827 |
| BciT130I | CCWGG | 1 cut(s) | 902 |
| BciVI | GTATCC | 2 cut(s) | 1829, 1985 |
| BcoDI | GTCTC | 7 cut(s) | 350, 392, 811, 1219, 1240, 1939, 1946 |
| BfaI | CTAG | 7 cut(s) | 419, 648, 1146, 1298, 1667, 2336, 2470 |
| BfmI | CTRYAG | 3 cut(s) | 1015, 2019, 2267 |
| BfuI | GTATCC | 2 cut(s) | 1829, 1985 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 43 |
| BglII | AGATCT | 1 cut(s) | 650 |
| BisI | GCNGC | 7 cut(s) | 238, 615, 618, 627, 1740, 2112, 2267 |
| BlsI | GCNGC | 7 cut(s) | 239, 616, 619, 628, 1741, 2113, 2268 |
| BmcAI | AGTACT | 1 cut(s) | 2009 |
| Bme1390I | CCNGG | 1 cut(s) | 902 |
| Bme18I | GGWCC | 1 cut(s) | 128 |
| BmgBI | CACGTC | 1 cut(s) | 796 |
| BmgT120I | GGNCC | 1 cut(s) | 128 |
| BmiI | GGNNCC | 3 cut(s) | 36, 2186, 2427 |
| BmrFI | CCNGG | 1 cut(s) | 902 |
| BmrI | ACTGGG | 2 cut(s) | 2140, 2158 |
| BmsI | GCATC | 4 cut(s) | 480, 499, 736, 1879 |
| BmuI | ACTGGG | 2 cut(s) | 2140, 2158 |
| BoxI | GACNNNNGTC | 1 cut(s) | 2151 |
| BpiI | GAAGAC | 1 cut(s) | 2147 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 808, 840 |
| BpmI | CTGGAG | 2 cut(s) | 884, 1468 |
| Bpu10I | CCTNAGC | 1 cut(s) | 2252 |
| Bpu14I | TTCGAA | 2 cut(s) | 1043, 1813 |
| BpuEI | CTTGAG | 6 cut(s) | 515, 654, 1243, 1975, 1988, 2056 |
| BsaHI | GRCGYC | 1 cut(s) | 374 |
| BsaI | GGTCTC | 2 cut(s) | 811, 1219 |
| BsaJI | CCNNGG | 5 cut(s) | 226, 557, 1123, 2180, 2350 |
| BsaWI | WCCGGW | 2 cut(s) | 788, 1692 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 865, 1988 |
| Bse118I | RCCGGY | 2 cut(s) | 641, 1692 |
| Bse1I | ACTGG | 6 cut(s) | 1451, 1654, 1822, 2135, 2153, 2331 |
| BseBI | CCWGG | 1 cut(s) | 902 |
| BseDI | CCNNGG | 5 cut(s) | 226, 557, 1123, 2180, 2350 |
| BseGI | GGATG | 5 cut(s) | 514, 541, 1289, 1923, 2458 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 865, 1988 |
| BseMII | CTCAG | 5 cut(s) | 288, 935, 979, 2115, 2266 |
| BseNI | ACTGG | 6 cut(s) | 1451, 1654, 1822, 2135, 2153, 2331 |
| BseRI | GAGGAG | 3 cut(s) | 311, 402, 1734 |
| BseX3I | CGGCCG | 1 cut(s) | 618 |
| BseXI | GCAGC | 4 cut(s) | 613, 1726, 2098, 2253 |
| BseYI | CCCAGC | 2 cut(s) | 812, 2090 |
| BsgI | GTGCAG | 1 cut(s) | 1397 |
| Bsh1236I | CGCG | 3 cut(s) | 972, 2231, 2261 |
| Bsh1285I | CGRYCG | 2 cut(s) | 621, 1697 |
| BshFI | GGCC | 5 cut(s) | 556, 620, 905, 1353, 2001 |
| BshNI | GGYRCC | 1 cut(s) | 34 |
| BshTI | ACCGGT | 1 cut(s) | 1692 |
| BsiEI | CGRYCG | 2 cut(s) | 621, 1697 |
| BsiHKAI | GWGCWC | 1 cut(s) | 670 |
| BsiSI | CCGG | 6 cut(s) | 38, 412, 642, 777, 789, 1693 |
| BsiWI | CGTACG | 1 cut(s) | 569 |
| BslFI | GGGAC | 2 cut(s) | 2147, 2189 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 865, 1988 |
| BsmAI | GTCTC | 7 cut(s) | 350, 392, 811, 1219, 1240, 1939, 1946 |
| BsmBI | CGTCTC | 4 cut(s) | 350, 392, 1939, 1946 |
| BsmFI | GGGAC | 2 cut(s) | 2147, 2189 |
| BsmI | GAATGC | 1 cut(s) | 182 |
| BsnI | GGCC | 5 cut(s) | 556, 620, 905, 1353, 2001 |
| Bso31I | GGTCTC | 2 cut(s) | 811, 1219 |
| Bsp119I | TTCGAA | 2 cut(s) | 1043, 1813 |
| Bsp1286I | GDGCHC | 1 cut(s) | 670 |
| Bsp143I | GATC | 9 cut(s) | 261, 650, 701, 841, 878, 1202, 1783, 2094, 2235 |
| Bsp68I | TCGCGA | 1 cut(s) | 2231 |
| BspACI | CCGC | 9 cut(s) | 189, 237, 240, 614, 617, 639, 1453, 2207, 2347 |
| BspANI | GGCC | 5 cut(s) | 556, 620, 905, 1353, 2001 |
| BspCNI | CTCAG | 5 cut(s) | 287, 936, 978, 2114, 2265 |
| BspFNI | CGCG | 3 cut(s) | 972, 2231, 2261 |
| BspLI | GGNNCC | 3 cut(s) | 36, 2186, 2427 |
| BspMAI | CTGCAG | 1 cut(s) | 2271 |
| BspPI | GGATC | 4 cut(s) | 709, 849, 873, 2102 |
| BspQI | GCTCTTC | 1 cut(s) | 1815 |
| BspT104I | TTCGAA | 2 cut(s) | 1043, 1813 |
| BspT107I | GGYRCC | 1 cut(s) | 34 |
| BspTNI | GGTCTC | 2 cut(s) | 811, 1219 |
| BsrFI | RCCGGY | 2 cut(s) | 641, 1692 |
| BsrI | ACTGG | 6 cut(s) | 1451, 1654, 1822, 2135, 2153, 2331 |
| BssAI | RCCGGY | 2 cut(s) | 641, 1692 |
| BssECI | CCNNGG | 5 cut(s) | 226, 557, 1123, 2180, 2350 |
| BssMI | GATC | 9 cut(s) | 261, 650, 701, 841, 878, 1202, 1783, 2094, 2235 |
| BssNAI | GTATAC | 1 cut(s) | 2221 |
| BssNI | GRCGYC | 1 cut(s) | 374 |
| BssT1I | CCWWGG | 3 cut(s) | 557, 1123, 2180 |
| Bst1107I | GTATAC | 1 cut(s) | 2221 |
| Bst2UI | CCWGG | 1 cut(s) | 902 |
| Bst6I | CTCTTC | 3 cut(s) | 177, 1815, 1965 |
| BstACI | GRCGYC | 1 cut(s) | 374 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 34, 2275 |
| BstBI | TTCGAA | 2 cut(s) | 1043, 1813 |
| BstC8I | GCNNGC | 2 cut(s) | 1083, 1806 |
| BstDEI | CTNAG | 7 cut(s) | 274, 944, 965, 2101, 2137, 2172, 2252 |
| BstDSI | CCRYGG | 2 cut(s) | 226, 2350 |
| BstF5I | GGATG | 5 cut(s) | 514, 541, 1289, 1923, 2458 |
| BstFNI | CGCG | 3 cut(s) | 972, 2231, 2261 |
| BstHHI | GCGC | 2 cut(s) | 322, 974 |
| BstKTI | GATC | 9 cut(s) | 264, 653, 704, 844, 881, 1205, 1786, 2097, 2238 |
| BstMAI | GTCTC | 7 cut(s) | 350, 392, 811, 1219, 1240, 1939, 1946 |
| BstMBI | GATC | 9 cut(s) | 261, 650, 701, 841, 878, 1202, 1783, 2094, 2235 |
| BstMCI | CGRYCG | 2 cut(s) | 621, 1697 |
| BstNI | CCWGG | 1 cut(s) | 902 |
| BstNSI | RCATGY | 2 cut(s) | 314, 1085 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 2151 |
| BstSCI | CCNGG | 1 cut(s) | 900 |
| BstSFI | CTRYAG | 3 cut(s) | 1015, 2019, 2267 |
| BstUI | CGCG | 3 cut(s) | 972, 2231, 2261 |
| BstV1I | GCAGC | 4 cut(s) | 613, 1726, 2098, 2253 |
| BstV2I | GAAGAC | 1 cut(s) | 2147 |
| BstX2I | RGATCY | 2 cut(s) | 650, 2094 |
| BstYI | RGATCY | 2 cut(s) | 650, 2094 |
| BstZ17I | GTATAC | 1 cut(s) | 2221 |
| BstZI | CGGCCG | 1 cut(s) | 618 |
| BsuI | GTATCC | 2 cut(s) | 1829, 1985 |
| BsuRI | GGCC | 5 cut(s) | 556, 620, 905, 1353, 2001 |
| BtgI | CCRYGG | 2 cut(s) | 226, 2350 |
| BtgZI | GCGATG | 2 cut(s) | 2212, 2246 |
| BtrI | CACGTC | 1 cut(s) | 796 |
| BtsCI | GGATG | 5 cut(s) | 514, 541, 1289, 1923, 2458 |
| BtsI | GCAGTG | 2 cut(s) | 315, 1729 |
| BtsIMutI | CAGTG | 5 cut(s) | 315, 1647, 1729, 1955, 2056 |
| BtuMI | TCGCGA | 1 cut(s) | 2231 |
| Cac8I | GCNNGC | 2 cut(s) | 1083, 1806 |
| CfoI | GCGC | 2 cut(s) | 322, 974 |
| Cfr10I | RCCGGY | 2 cut(s) | 641, 1692 |
| Cfr13I | GGNCC | 1 cut(s) | 128 |
| CseI | GACGC | 2 cut(s) | 363, 978 |
| Csp6I | GTAC | 6 cut(s) | 394, 570, 1363, 1497, 2008, 2124 |
| CspAI | ACCGGT | 1 cut(s) | 1692 |
| CviQI | GTAC | 6 cut(s) | 394, 570, 1363, 1497, 2008, 2124 |
| DdeI | CTNAG | 7 cut(s) | 274, 944, 965, 2101, 2137, 2172, 2252 |
| DpnI | GATC | 9 cut(s) | 263, 652, 703, 843, 880, 1204, 1785, 2096, 2237 |
| DpnII | GATC | 9 cut(s) | 261, 650, 701, 841, 878, 1202, 1783, 2094, 2235 |
| EaeI | YGGCCR | 1 cut(s) | 618 |
| EagI | CGGCCG | 1 cut(s) | 618 |
| Eam1104I | CTCTTC | 3 cut(s) | 177, 1815, 1965 |
| EarI | CTCTTC | 3 cut(s) | 177, 1815, 1965 |
| EciI | GGCGGA | 2 cut(s) | 255, 2336 |
| Ecl136II | GAGCTC | 1 cut(s) | 668 |
| EclXI | CGGCCG | 1 cut(s) | 618 |
| Eco130I | CCWWGG | 3 cut(s) | 557, 1123, 2180 |
| Eco147I | AGGCCT | 1 cut(s) | 905 |
| Eco24I | GRGCYC | 1 cut(s) | 670 |
| Eco31I | GGTCTC | 2 cut(s) | 811, 1219 |
| Eco47I | GGWCC | 1 cut(s) | 128 |
| Eco52I | CGGCCG | 1 cut(s) | 618 |
| Eco53kI | GAGCTC | 1 cut(s) | 668 |
| Eco57I | CTGAAG | 2 cut(s) | 1931, 2353 |
| EcoICRI | GAGCTC | 1 cut(s) | 668 |
| EcoRII | CCWGG | 1 cut(s) | 900 |
| EcoT14I | CCWWGG | 3 cut(s) | 557, 1123, 2180 |
| EcoT38I | GRGCYC | 1 cut(s) | 670 |
| ErhI | CCWWGG | 3 cut(s) | 557, 1123, 2180 |
| Esp3I | CGTCTC | 4 cut(s) | 350, 392, 1939, 1946 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 1069, 1101, 1997, 2029 |
| FaqI | GGGAC | 2 cut(s) | 2147, 2189 |
| FauI | CCCGC | 2 cut(s) | 646, 2214 |
| FblI | GTMKAC | 2 cut(s) | 2055, 2220 |
| Fnu4HI | GCNGC | 7 cut(s) | 238, 615, 618, 627, 1740, 2112, 2267 |
| FokI | GGATG | 5 cut(s) | 521, 528, 1296, 1910, 2465 |
| FriOI | GRGCYC | 1 cut(s) | 670 |
| Fsp4HI | GCNGC | 7 cut(s) | 238, 615, 618, 627, 1740, 2112, 2267 |
| FspBI | CTAG | 7 cut(s) | 419, 648, 1146, 1298, 1667, 2336, 2470 |
| GlaI | GCGC | 2 cut(s) | 321, 973 |
| GluI | GCNGC | 7 cut(s) | 238, 615, 618, 627, 1740, 2112, 2267 |
| GsaI | CCCAGC | 2 cut(s) | 816, 2094 |
| GsuI | CTGGAG | 2 cut(s) | 884, 1468 |
| HaeIII | GGCC | 5 cut(s) | 556, 620, 905, 1353, 2001 |
| HapII | CCGG | 6 cut(s) | 38, 412, 642, 777, 789, 1693 |
| HgaI | GACGC | 2 cut(s) | 363, 978 |
| HhaI | GCGC | 2 cut(s) | 322, 974 |
| Hin1I | GRCGYC | 1 cut(s) | 374 |
| Hin6I | GCGC | 2 cut(s) | 320, 972 |
| HinP1I | GCGC | 2 cut(s) | 320, 972 |
| HincII | GTYRAC | 3 cut(s) | 1591, 1791, 2056 |
| HindII | GTYRAC | 3 cut(s) | 1591, 1791, 2056 |
| HindIII | AAGCTT | 2 cut(s) | 51, 995 |
| HpaI | GTTAAC | 1 cut(s) | 1791 |
| HpaII | CCGG | 6 cut(s) | 38, 412, 642, 777, 789, 1693 |
| HphI | GGTGA | 3 cut(s) | 167, 198, 803 |
| Hpy166II | GTNNAC | 4 cut(s) | 1591, 1791, 2056, 2221 |
| Hpy188III | TCNNGA | 8 cut(s) | 10, 545, 1222, 1577, 2014, 2230, 2239, 2336 |
| Hpy8I | GTNNAC | 4 cut(s) | 1591, 1791, 2056, 2221 |
| Hpy99I | CGWCG | 7 cut(s) | 28, 364, 379, 626, 1409, 2060, 2450 |
| HpyAV | CCTTC | 7 cut(s) | 377, 689, 859, 985, 1343, 1955, 2305 |
| HpyCH4IV | ACGT | 5 cut(s) | 362, 568, 795, 1172, 1932 |
| HpyCH4V | TGCA | 8 cut(s) | 749, 1342, 1414, 1553, 1804, 1878, 2269, 2302 |
| HpyF3I | CTNAG | 7 cut(s) | 274, 944, 965, 2101, 2137, 2172, 2252 |
| HpySE526I | ACGT | 5 cut(s) | 362, 568, 795, 1172, 1932 |
| Hsp92I | GRCGYC | 1 cut(s) | 374 |
| HspAI | GCGC | 2 cut(s) | 320, 972 |
| KspAI | GTTAAC | 1 cut(s) | 1791 |
| Kzo9I | GATC | 9 cut(s) | 261, 650, 701, 841, 878, 1202, 1783, 2094, 2235 |
| LguI | GCTCTTC | 1 cut(s) | 1815 |
| LmnI | GCTCC | 4 cut(s) | 665, 919, 1747, 2190 |
| Lsp1109I | GCAGC | 4 cut(s) | 613, 1726, 2098, 2253 |
| LweI | GCATC | 4 cut(s) | 480, 499, 736, 1879 |
| MaeI | CTAG | 7 cut(s) | 419, 648, 1146, 1298, 1667, 2336, 2470 |
| MaeII | ACGT | 5 cut(s) | 362, 568, 795, 1172, 1932 |
| MaeIII | GTNAC | 7 cut(s) | 313, 350, 791, 1002, 1141, 1433, 1756 |
| MalI | GATC | 9 cut(s) | 263, 652, 703, 843, 880, 1204, 1785, 2096, 2237 |
| MboI | GATC | 9 cut(s) | 261, 650, 701, 841, 878, 1202, 1783, 2094, 2235 |
| MflI | RGATCY | 2 cut(s) | 650, 2094 |
| MhlI | GDGCHC | 1 cut(s) | 670 |
| MlyI | GAGTC | 7 cut(s) | 163, 352, 577, 1143, 1554, 1708, 1892 |
| MmeI | TCCRAC | 2 cut(s) | 357, 643 |
| MroXI | GAANNNNTTC | 3 cut(s) | 17, 1629, 2366 |
| MslI | CAYNNNNRTG | 6 cut(s) | 249, 546, 1103, 1421, 1652, 2231 |
| MspA1I | CMGCKG | 1 cut(s) | 189 |
| MspI | CCGG | 6 cut(s) | 38, 412, 642, 777, 789, 1693 |
| MspR9I | CCNGG | 1 cut(s) | 902 |
| Mva1269I | GAATGC | 1 cut(s) | 182 |
| MvaI | CCWGG | 1 cut(s) | 902 |
| MvnI | CGCG | 3 cut(s) | 972, 2231, 2261 |
| NdeII | GATC | 9 cut(s) | 261, 650, 701, 841, 878, 1202, 1783, 2094, 2235 |
| NlaIV | GGNNCC | 3 cut(s) | 36, 2186, 2427 |
| NmeAIII | GCCGAG | 1 cut(s) | 317 |
| NmuCI | GTSAC | 2 cut(s) | 313, 791 |
| NruI | TCGCGA | 1 cut(s) | 2231 |
| NspI | RCATGY | 2 cut(s) | 314, 1085 |
| NspV | TTCGAA | 2 cut(s) | 1043, 1813 |
| OliI | CACNNNNGTG | 1 cut(s) | 249 |
| PaeI | GCATGC | 1 cut(s) | 1085 |
| PceI | AGGCCT | 1 cut(s) | 905 |
| PciSI | GCTCTTC | 1 cut(s) | 1815 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 1404, 2454 |
| PctI | GAATGC | 1 cut(s) | 182 |
| PdmI | GAANNNNTTC | 3 cut(s) | 17, 1629, 2366 |
| PfeI | GAWTC | 3 cut(s) | 278, 325, 940 |
| Pfl23II | CGTACG | 1 cut(s) | 569 |
| PflFI | GACNNNGTC | 1 cut(s) | 2138 |
| PinAI | ACCGGT | 1 cut(s) | 1692 |
| PkrI | GCNGC | 7 cut(s) | 239, 616, 619, 628, 1741, 2113, 2268 |
| PleI | GAGTC | 7 cut(s) | 163, 351, 577, 1143, 1553, 1707, 1891 |
| PpsI | GAGTC | 7 cut(s) | 163, 351, 577, 1143, 1553, 1707, 1891 |
| PshAI | GACNNNNGTC | 1 cut(s) | 2151 |
| PsiI | TTATAA | 1 cut(s) | 453 |
| Psp124BI | GAGCTC | 1 cut(s) | 670 |
| Psp6I | CCWGG | 1 cut(s) | 900 |
| PspFI | CCCAGC | 2 cut(s) | 812, 2090 |
| PspGI | CCWGG | 1 cut(s) | 900 |
| PspLI | CGTACG | 1 cut(s) | 569 |
| PspN4I | GGNNCC | 3 cut(s) | 36, 2186, 2427 |
| PspPI | GGNCC | 1 cut(s) | 128 |
| PstI | CTGCAG | 1 cut(s) | 2271 |
| PsuI | RGATCY | 2 cut(s) | 650, 2094 |
| PsyI | GACNNNGTC | 1 cut(s) | 2138 |
| RruI | TCGCGA | 1 cut(s) | 2231 |
| RsaI | GTAC | 6 cut(s) | 395, 571, 1364, 1498, 2009, 2125 |
| RsaNI | GTAC | 6 cut(s) | 394, 570, 1363, 1497, 2008, 2124 |
| RseI | CAYNNNNRTG | 6 cut(s) | 249, 546, 1103, 1421, 1652, 2231 |
| SacI | GAGCTC | 1 cut(s) | 670 |
| SalI | GTCGAC | 1 cut(s) | 2054 |
| SapI | GCTCTTC | 1 cut(s) | 1815 |
| SatI | GCNGC | 7 cut(s) | 238, 615, 618, 627, 1740, 2112, 2267 |
| Sau3AI | GATC | 9 cut(s) | 261, 650, 701, 841, 878, 1202, 1783, 2094, 2235 |
| Sau96I | GGNCC | 1 cut(s) | 128 |
| ScaI | AGTACT | 1 cut(s) | 2009 |
| SchI | GAGTC | 7 cut(s) | 163, 352, 577, 1143, 1554, 1708, 1892 |
| ScrFI | CCNGG | 1 cut(s) | 902 |
| SduI | GDGCHC | 1 cut(s) | 670 |
| SfaNI | GCATC | 4 cut(s) | 480, 499, 736, 1879 |
| SfcI | CTRYAG | 3 cut(s) | 1015, 2019, 2267 |
| SfuI | TTCGAA | 2 cut(s) | 1043, 1813 |
| SgrAI | CRCCGGYG | 1 cut(s) | 641 |
| SinI | GGWCC | 1 cut(s) | 128 |
| SmiMI | CAYNNNNRTG | 6 cut(s) | 249, 546, 1103, 1421, 1652, 2231 |
| SmlI | CTYRAG | 6 cut(s) | 494, 669, 1222, 1954, 2003, 2035 |
| SmoI | CTYRAG | 6 cut(s) | 494, 669, 1222, 1954, 2003, 2035 |
| SphI | GCATGC | 1 cut(s) | 1085 |
| SseBI | AGGCCT | 1 cut(s) | 905 |
| SsiI | CCGC | 9 cut(s) | 189, 237, 240, 614, 617, 639, 1453, 2207, 2347 |
| SspI | AATATT | 1 cut(s) | 2085 |
| SspMI | CTAG | 7 cut(s) | 419, 648, 1146, 1298, 1667, 2336, 2470 |
| SstI | GAGCTC | 1 cut(s) | 670 |
| StuI | AGGCCT | 1 cut(s) | 905 |
| StyD4I | CCNGG | 1 cut(s) | 900 |
| StyI | CCWWGG | 3 cut(s) | 557, 1123, 2180 |
| TaiI | ACGT | 5 cut(s) | 365, 571, 798, 1175, 1935 |
| TatI | WGTACW | 3 cut(s) | 1362, 2007, 2123 |
| TauI | GCSGC | 3 cut(s) | 240, 617, 620 |
| TfiI | GAWTC | 3 cut(s) | 278, 325, 940 |
| TscAI | CASTG | 5 cut(s) | 322, 1654, 1736, 1955, 2056 |
| TseFI | GTSAC | 2 cut(s) | 313, 791 |
| TseI | GCWGC | 4 cut(s) | 626, 1739, 2111, 2266 |
| Tsp45I | GTSAC | 2 cut(s) | 313, 791 |
| TspGWI | ACGGA | 3 cut(s) | 243, 932, 2177 |
| TspRI | CASTG | 5 cut(s) | 322, 1654, 1736, 1955, 2056 |
| Tth111I | GACNNNGTC | 1 cut(s) | 2138 |
| VpaK11BI | GGWCC | 1 cut(s) | 128 |
| XapI | RAATTY | 4 cut(s) | 101, 910, 926, 2362 |
| XbaI | TCTAGA | 1 cut(s) | 2335 |
| XceI | RCATGY | 2 cut(s) | 314, 1085 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 1273, 1579 |
| XmiI | GTMKAC | 2 cut(s) | 2055, 2220 |
| XmnI | GAANNNNTTC | 3 cut(s) | 17, 1629, 2366 |
| XspI | CTAG | 7 cut(s) | 419, 648, 1146, 1298, 1667, 2336, 2470 |
| ZrmI | AGTACT | 1 cut(s) | 2009 |