Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr00

Orthogroup ID

OG0001425

Physical Location & Seq

Genomic Coordinates Reverse (-)

47531487 .. 47534671

Gene Structure 3185 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD00G1200800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2103 bp

ATGAAGTTCGGGAAGGAATTTTCTGCACAAATGGTGCCGGAATGGAAAGACGCATACATGGATTACGACTATCTAAAATCCCTCTTAAAAGAAATTCAAGTCTGCAAGCAAAGAAACAACCCCGACCCACAGGCCGCCGCCTCGCCCCGCAGCTACGGCCTAAAGCGCAGGTTCACGTTGTACAGATCCTTCAGCGGTCTGACAAAAAGTAGGCACACCCAGCAGCCCGCCAGCCCGTCTTCCACCTCCGACATTGAGAGCCAGGCCATTCTCGTCAACTCCGTCAACGAAAACGGCTCTGAGAACTACCAGACGATGTTTCTGATGGCGGCGGAGGAAGGCGGAGTTCAGGAGCTGGAGTACTTCAAGAGGCTTGACGACGAGTTCAATAAAGTGGACAAGTTTTATAGGTCGAAGGTTGACGAGGTGATGAAGGAAGCTGCGGTTCTTAACAAGCAAATGGACGCTTTGATTGCGTTTCGGATCAAAGTGGAGAACCCTCAGAGAACGTTTGATTGGTCAGGGGGGATGACTCGCCTTGCTTCCGATGTTGCCACGTCTACAGCTGCATTGGCTGCAGCCACCCCATGCAGTGCCAGAGCAAGCAGGCGGGTACTGGCTATGGATGCGATTGAAGAGAGCGGATTAAACAGTTCGGGTGACGAAGAAAAAGAAGACAAAAGGAGTGGAGACGTTGAGATGAAGGTGAAGAAGCCGGAGAGCTTGAGAGGCACTAGGCCTACGCCACTGGATATACTGGACCAGGTGACAATGAATCACATGGCTGAGACGCCGCGTTCCACCATCAAAGGCTTCCTCAACGTGGCTCCACATACGGAGCTCAAGTTCAGCAGGGCTAATCTCAGCAAAGTTGAACAACAGCTCAGGCGCGCTTTCATTGAATTTTACCAGAAACTTCGGCTTCTGAAGAATTTTTTGAATACGTTGGCTTTCTCAAAGATCATGAAGAAATACGATAAGGTGGCTTCAAGAGATGCATCAAAATCTTACATGAACATGGTGGATAACTCGTACCTTGGCAGCTCCGACGAGATTACCAAGCTTATGGAAAGGGTTGAAACTACGTTCATCAAGCATTTCTCGAACTCCAATCGCCGAAAAGGAATGGCGGTCTTGAGGCCAAAACCGAGGGTAGAAAAGACATCGGACAACATTCGCTATGGCTTGTTCGGATTCATAGTTCTCCACATGCTTATGTATGCCGGAAATATATACTTTTGGCGGCGGTTCAGAGTCAATTACTCTTTCATATTCGGTTTCAAGCAAGGAACCGAGTTGGGATACAGAGAGATCCTCCTTCTGAGTTCTGGTCTGGCAATGTTAGCACTAGCCTCTGTGCTCGCAAATCTTGACATGGAGATGGACCCGAAAACCAATGATTACAAAGCGCTAACCGAGCTTCTCCCACTCTTCTTGCTCCTGGTCACACTCCCGGATTTCTTCTTGGCAGATCAGTTAACTAGCCAGACTTCAAAGTCAGAGAAACAACTTGCACATCAGATGACGTCTTCAAAAATTTTTACTTTCATCGTGGCGTGCGTTCCATATTGGTCCCGCCTCCTTCAGTGCATCCGCCGTTTGGTTGAAGAGAAAGATCCAATGCAAGGTTATAATGGACTGAAATATTTCTTCACCATCGTAGCTGTCAGCATGAGAATAGCCTACACTCATAACAAAGACGTGAACTGGAAAATTCTTGCTGGGATTTTCTCAATTGTTGCAGCCATCTATGGAACATACTGGGATCTTGTCGTGGACTGGGGACTTCTGCAACGCCGCTCCAAGAATCGATGGTTGAGAGACAAACTCTTGGTCCCTTACAAAAGCGTATACTTCATAGCCATGGTGTTGAATGTGCTGCTGAGATTTGCCTGGCTGCAGACAGTTTTGAATTTCAGTGTCTCCTTCATGCATACACAGACAATGATCTCGGTTGTGGCGAGCTTGGAGATAATTCGTCGAGGGATATGGAGTTTTTTCCGGTTGGAGAACGAGCATCTCAACAATGTCGGAAAGTACAGAGCATTCAAGTCAGTGCCACTGCCCTTCAACTATGATGAAGACGAATGCAAAGATGAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0005802 GO:0005886 GO:0008150 GO:0012505 GO:0016020 GO:0031984 GO:0042592 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0048878 GO:0050801 GO:0055062 GO:0055065 GO:0055069 GO:0055076 GO:0055080 GO:0055081 GO:0055083 GO:0065007 GO:0065008 GO:0071944 GO:0072505 GO:0072506 GO:0072507 GO:0098771 GO:0098791

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

EXS SPX

Protein Analysis

701

Amino Acids

80.65

Weight (kDa)

9.35

Isoelectric Point (pI)

43.4

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SPX	PF03105	1 - 341	3.4e-82	SPX domain
EXS	PF03124	394 - 483	4.8e-13	EXS family
EXS	PF03124	482 - 674	1.9e-56	EXS family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001425)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G14040 AT1G26730 AT1G26730 AT1G35350 AT1G35350 AT2G03240 AT2G03260 AT2G03260
fragaria_vesca	FvH4_4g21230 FvH4_4g30900 FvH4_4g30930 FvH4_4g30930 FvH4_4g30930 FvH4_4g30930 FvH4_4g30930 FvH4_4g30930 FvH4_4g30930 FvH4_4g30930 FvH4_4g30930 FvH4_4g30930 FvH4_4g30930 FvH4_4g30930 FvH4_4g30930 FvH4_4g30930
malus_domestica	MD00G1081000.v1.1 MD00G1081300.v1.1 MD00G1200800.v1.1 MD16G1068000.v1.1
prunus_persica	Prupe.1G278700_v2.0.a1 Prupe.1G278700_v2.0.a1 Prupe.1G278700_v2.0.a1 Prupe.1G278800_v2.0.a1
pyrus_communis	pycom13g06110 pycom13g06120 pycom16g05960
rosa_chinensis	RchiOBHm_Chr4g0439231
rosa_laevigata	RLG00000006286
rosa_multiflora	Rmu_sc0006598.1_g000012 Rmu_sc0007941.1_g000004 Rmu_ssc0000171.1_g000025
rosa_roxburghii	Rroxscaffold_2G00115520 Rroxscaffold_2G00115990 Rroxscaffold_5G00380220
rosa_rugosa	Rorug04G0317100 Rorug04G0317200
rosa_samantha	Rh4AG367700 Rh4BG379600 Rh4CG393900 Rh4DG373900
rosa_wichuraiana	Rw0G005620 Rw0G011630 Rw5G028550

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1632
AasI	GACNNNNNNGTC	1 cut(s)	1495
AatII	GACGTC	1 cut(s)	1529
Acc36I	ACCTGC	1 cut(s)	159
AccB1I	GGYRCC	1 cut(s)	34
AccBSI	CCGCTC	2 cut(s)	642, 1800
AccI	GTMKAC	2 cut(s)	560, 1851
AccII	CGCG	2 cut(s)	796, 891
AclI	AACGTT	1 cut(s)	509
AclWI	GGATC	5 cut(s)	180, 491, 1306, 1610, 1773
AcsI	RAATTY	7 cut(s)	17, 93, 902, 931, 1536, 1713, 1912
AcuI	CTGAAG	3 cut(s)	175, 947, 1568
AcyI	GRCGYC	2 cut(s)	791, 1526
AfaI	GTAC	5 cut(s)	182, 362, 615, 1034, 2039
AfeI	AGCGCT	1 cut(s)	1410
AfiI	CCNNNNNNNGG	3 cut(s)	823, 1600, 1625
AjiI	CACGTC	2 cut(s)	558, 1702
AjnI	CCWGG	4 cut(s)	261, 762, 1440, 1892
AjuI	GAANNNNNNNTTGG	2 cut(s)	499, 531
AloI	GAACNNNNNNTCC	4 cut(s)	830, 862, 1185, 1217
Alw21I	GWGCWC	2 cut(s)	843, 1362
Alw26I	GTCTC	4 cut(s)	684, 782, 1815, 1927
AlwI	GGATC	5 cut(s)	180, 491, 1306, 1610, 1773
AlwNI	CAGNNNCTG	1 cut(s)	355
Aor51HI	AGCGCT	1 cut(s)	1410
AoxI	GGCC	5 cut(s)	132, 157, 264, 737, 1139
ApoI	RAATTY	7 cut(s)	17, 93, 902, 931, 1536, 1713, 1912
ArsI	GACNNNNNNTTYG	2 cut(s)	1818, 1850
Asp700I	GAANNNNTTC	1 cut(s)	1646
AspLEI	GCGC	4 cut(s)	168, 891, 893, 1411
AspS9I	GGNCC	4 cut(s)	760, 1384, 1572, 1834
AsuC2I	CCSGG	1 cut(s)	1454
AsuHPI	GGTGA	5 cut(s)	439, 671, 718, 778, 1645
AvaII	GGWCC	4 cut(s)	760, 1384, 1572, 1834
BanI	GGYRCC	1 cut(s)	34
BanII	GRGCYC	1 cut(s)	843
BarI	GAAGNNNNNNTAC	2 cut(s)	173, 205
BbsI	GAAGAC	4 cut(s)	231, 681, 1521, 2088
Bbv12I	GWGCWC	2 cut(s)	843, 1362
BccI	CCATC	6 cut(s)	319, 812, 1375, 1664, 1754, 1806
BceAI	ACGGC	3 cut(s)	172, 310, 1581
BciT130I	CCWGG	4 cut(s)	263, 764, 1442, 1894
BciVI	GTATCC	1 cut(s)	1295
BcnI	CCSGG	1 cut(s)	1454
BcoDI	GTCTC	4 cut(s)	684, 782, 1815, 1927
BfaI	CTAG	3 cut(s)	735, 1349, 1482
BfmI	CTRYAG	3 cut(s)	561, 576, 1898
BfoI	RGCGCY	1 cut(s)	1412
BfuAI	ACCTGC	1 cut(s)	159
BfuI	GTATCC	1 cut(s)	1295
BmcAI	AGTACT	1 cut(s)	362
Bme1390I	CCNGG	5 cut(s)	263, 764, 1442, 1454, 1894
Bme18I	GGWCC	4 cut(s)	760, 1384, 1572, 1834
BmgBI	CACGTC	2 cut(s)	558, 1702
BmgT120I	GGNCC	4 cut(s)	760, 1384, 1572, 1834
BmiI	GGNNCC	6 cut(s)	36, 828, 1291, 1386, 1574, 1836
BmrFI	CCNGG	5 cut(s)	263, 764, 1442, 1454, 1894
BmrI	ACTGGG	2 cut(s)	1771, 1789
BmsI	GCATC	5 cut(s)	616, 985, 1007, 1599, 2026
BmuI	ACTGGG	2 cut(s)	1771, 1789
BpiI	GAAGAC	4 cut(s)	231, 681, 1521, 2088
BplI	GAGNNNNNCTC	4 cut(s)	1299, 1331, 1812, 1844
BpmI	CTGGAG	1 cut(s)	377
Bpu10I	CCTNAGC	1 cut(s)	884
BpuEI	CTTGAG	3 cut(s)	745, 827, 1156
BpuMI	CCSGG	1 cut(s)	1454
Bsa29I	ATCGAT	1 cut(s)	1810
BsaHI	GRCGYC	2 cut(s)	791, 1526
BsaJI	CCNNGG	3 cut(s)	1036, 1148, 1863
BsaWI	WCCGGW	1 cut(s)	2001
BsaXI	ACNNNNNCTCC	4 cut(s)	344, 374, 830, 860
Bsc4I	CCNNNNNNNGG	3 cut(s)	823, 1600, 1625
Bse1I	ACTGG	6 cut(s)	621, 753, 762, 1712, 1766, 1784
Bse3DI	GCAATG	1 cut(s)	1344
BseBI	CCWGG	4 cut(s)	263, 764, 1442, 1894
BseCI	ATCGAT	1 cut(s)	1810
BseDI	CCNNGG	3 cut(s)	1036, 1148, 1863
BseGI	GGATG	3 cut(s)	534, 631, 1590
BseLI	CCNNNNNNNGG	3 cut(s)	823, 1600, 1625
BseMI	GCAATG	1 cut(s)	1344
BseMII	CTCAG	7 cut(s)	291, 515, 777, 877, 898, 1313, 1874
BseNI	ACTGG	6 cut(s)	621, 753, 762, 1712, 1766, 1784
BsePI	GCGCGC	1 cut(s)	889
BseYI	CCCAGC	2 cut(s)	219, 1721
BsgI	GTGCAG	1 cut(s)	9
Bsh1236I	CGCG	2 cut(s)	796, 891
BshFI	GGCC	5 cut(s)	134, 159, 266, 739, 1141
BshNI	GGYRCC	1 cut(s)	34
BshVI	ATCGAT	1 cut(s)	1810
BsiHKAI	GWGCWC	2 cut(s)	843, 1362
BsiSI	CCGG	5 cut(s)	38, 716, 1224, 1454, 2002
BslFI	GGGAC	3 cut(s)	1558, 1797, 1820
BslI	CCNNNNNNNGG	3 cut(s)	823, 1600, 1625
BsmAI	GTCTC	4 cut(s)	684, 782, 1815, 1927
BsmBI	CGTCTC	2 cut(s)	684, 782
BsmFI	GGGAC	3 cut(s)	1558, 1797, 1820
BsmI	GAATGC	2 cut(s)	2045, 2093
BsnI	GGCC	5 cut(s)	134, 159, 266, 739, 1141
Bsp1286I	GDGCHC	2 cut(s)	843, 1362
Bsp1407I	TGTACA	1 cut(s)	180
Bsp143I	GATC	8 cut(s)	185, 483, 960, 1311, 1471, 1615, 1765, 1947
Bsp19I	CCATGG	1 cut(s)	1863
BspANI	GGCC	5 cut(s)	134, 159, 266, 739, 1141
BspCNI	CTCAG	7 cut(s)	292, 514, 778, 876, 897, 1314, 1875
BspDI	ATCGAT	1 cut(s)	1810
BspFNI	CGCG	2 cut(s)	796, 891
BspHI	TCATGA	1 cut(s)	963
BspLI	GGNNCC	6 cut(s)	36, 828, 1291, 1386, 1574, 1836
BspMAI	CTGCAG	2 cut(s)	580, 1902
BspMI	ACCTGC	1 cut(s)	159
BspPI	GGATC	5 cut(s)	180, 491, 1306, 1610, 1773
BspT107I	GGYRCC	1 cut(s)	34
BsrBI	CCGCTC	2 cut(s)	642, 1800
BsrDI	GCAATG	1 cut(s)	1344
BsrGI	TGTACA	1 cut(s)	180
BsrI	ACTGG	6 cut(s)	621, 753, 762, 1712, 1766, 1784
BssECI	CCNNGG	3 cut(s)	1036, 1148, 1863
BssHII	GCGCGC	1 cut(s)	889
BssMI	GATC	8 cut(s)	185, 483, 960, 1311, 1471, 1615, 1765, 1947
BssNAI	GTATAC	1 cut(s)	1852
BssNI	GRCGYC	2 cut(s)	791, 1526
BssT1I	CCWWGG	2 cut(s)	1036, 1863
Bst1107I	GTATAC	1 cut(s)	1852
Bst2UI	CCWGG	4 cut(s)	263, 764, 1442, 1894
Bst4CI	ACNGT	2 cut(s)	653, 1906
Bst6I	CTCTTC	3 cut(s)	630, 1436, 1602
BstACI	GRCGYC	2 cut(s)	791, 1526
BstAPI	GCANNNNNTGC	1 cut(s)	575
BstAUI	TGTACA	1 cut(s)	180
BstC8I	GCNNGC	9 cut(s)	107, 228, 232, 604, 608, 891, 1362, 1558, 1963
BstDEI	CTNAG	7 cut(s)	300, 501, 786, 863, 884, 1322, 1883
BstDSI	CCRYGG	1 cut(s)	1863
BstF5I	GGATG	3 cut(s)	534, 631, 1590
BstFNI	CGCG	2 cut(s)	796, 891
BstH2I	RGCGCY	1 cut(s)	1412
BstHHI	GCGC	4 cut(s)	168, 891, 893, 1411
BstKTI	GATC	8 cut(s)	188, 486, 963, 1314, 1474, 1618, 1768, 1950
BstMAI	GTCTC	4 cut(s)	684, 782, 1815, 1927
BstMBI	GATC	8 cut(s)	185, 483, 960, 1311, 1471, 1615, 1765, 1947
BstNI	CCWGG	4 cut(s)	263, 764, 1442, 1894
BstNSI	RCATGY	1 cut(s)	1213
BstSCI	CCNGG	5 cut(s)	261, 762, 1440, 1452, 1892
BstSFI	CTRYAG	3 cut(s)	561, 576, 1898
BstUI	CGCG	2 cut(s)	796, 891
BstV2I	GAAGAC	4 cut(s)	231, 681, 1521, 2088
BstX2I	RGATCY	4 cut(s)	185, 1311, 1615, 1765
BstXI	CCANNNNNNTGG	1 cut(s)	1066
BstYI	RGATCY	4 cut(s)	185, 1311, 1615, 1765
BstZ17I	GTATAC	1 cut(s)	1852
Bsu15I	ATCGAT	1 cut(s)	1810
BsuI	GTATCC	1 cut(s)	1295
BsuRI	GGCC	5 cut(s)	134, 159, 266, 739, 1141
BsuTUI	ATCGAT	1 cut(s)	1810
BtgI	CCRYGG	1 cut(s)	1863
BtrI	CACGTC	2 cut(s)	558, 1702
BtsCI	GGATG	3 cut(s)	534, 631, 1590
BtsI	GCAGTG	2 cut(s)	598, 2060
BtsIMutI	CAGTG	6 cut(s)	598, 746, 1592, 1924, 2060, 2061
BveI	ACCTGC	1 cut(s)	159
Cac8I	GCNNGC	9 cut(s)	107, 228, 232, 604, 608, 891, 1362, 1558, 1963
CaiI	CAGNNNCTG	1 cut(s)	355
CciI	TCATGA	1 cut(s)	963
CfoI	GCGC	4 cut(s)	168, 891, 893, 1411
Cfr13I	GGNCC	4 cut(s)	760, 1384, 1572, 1834
ClaI	ATCGAT	1 cut(s)	1810
CseI	GACGC	3 cut(s)	59, 473, 799
CsiI	ACCWGGT	1 cut(s)	762
Csp6I	GTAC	5 cut(s)	181, 361, 614, 1033, 2038
CspCI	CAANNNNNGTGG	4 cut(s)	667, 702, 1416, 1451
CviQI	GTAC	5 cut(s)	181, 361, 614, 1033, 2038
DdeI	CTNAG	7 cut(s)	300, 501, 786, 863, 884, 1322, 1883
DpnI	GATC	8 cut(s)	187, 485, 962, 1313, 1473, 1617, 1767, 1949
DpnII	GATC	8 cut(s)	185, 483, 960, 1311, 1471, 1615, 1765, 1947
DrdI	GACNNNNNNGTC	1 cut(s)	1495
DseDI	GACNNNNNNGTC	1 cut(s)	1495
Eam1104I	CTCTTC	3 cut(s)	630, 1436, 1602
EarI	CTCTTC	3 cut(s)	630, 1436, 1602
EciI	GGCGGA	3 cut(s)	347, 357, 1583
Ecl136II	GAGCTC	1 cut(s)	841
Eco130I	CCWWGG	2 cut(s)	1036, 1863
Eco147I	AGGCCT	1 cut(s)	739
Eco24I	GRGCYC	1 cut(s)	843
Eco47I	GGWCC	4 cut(s)	760, 1384, 1572, 1834
Eco47III	AGCGCT	1 cut(s)	1410
Eco53kI	GAGCTC	1 cut(s)	841
Eco57I	CTGAAG	3 cut(s)	175, 947, 1568
EcoICRI	GAGCTC	1 cut(s)	841
EcoRII	CCWGG	4 cut(s)	261, 762, 1440, 1892
EcoT14I	CCWWGG	2 cut(s)	1036, 1863
EcoT22I	ATGCAT	2 cut(s)	1000, 1935
EcoT38I	GRGCYC	1 cut(s)	843
ErhI	CCWWGG	2 cut(s)	1036, 1863
Esp3I	CGTCTC	2 cut(s)	684, 782
FaqI	GGGAC	3 cut(s)	1558, 1797, 1820
FauI	CCCGC	4 cut(s)	155, 235, 603, 1583
FblI	GTMKAC	2 cut(s)	560, 1851
FokI	GGATG	3 cut(s)	541, 638, 1577
FriOI	GRGCYC	1 cut(s)	843
FspBI	CTAG	3 cut(s)	735, 1349, 1482
GlaI	GCGC	4 cut(s)	167, 890, 892, 1410
GsaI	CCCAGC	2 cut(s)	223, 1725
GsuI	CTGGAG	1 cut(s)	377
HaeII	RGCGCY	1 cut(s)	1412
HaeIII	GGCC	5 cut(s)	134, 159, 266, 739, 1141
HapII	CCGG	5 cut(s)	38, 716, 1224, 1454, 2002
HgaI	GACGC	3 cut(s)	59, 473, 799
HhaI	GCGC	4 cut(s)	168, 891, 893, 1411
Hin1I	GRCGYC	2 cut(s)	791, 1526
Hin6I	GCGC	4 cut(s)	166, 889, 891, 1409
HinP1I	GCGC	4 cut(s)	166, 889, 891, 1409
HincII	GTYRAC	4 cut(s)	277, 286, 421, 1479
HindII	GTYRAC	4 cut(s)	277, 286, 421, 1479
HindIII	AAGCTT	1 cut(s)	1061
HinfI	GANTC	5 cut(s)	532, 775, 1194, 1254, 1807
HpaI	GTTAAC	1 cut(s)	1479
HpaII	CCGG	5 cut(s)	38, 716, 1224, 1454, 2002
HphI	GGTGA	5 cut(s)	439, 671, 718, 778, 1645
Hpy188III	TCNNGA	8 cut(s)	10, 350, 367, 964, 990, 1102, 1135, 1370
Hpy99I	CGWCG	3 cut(s)	383, 1052, 1983
HpyCH4III	ACNGT	2 cut(s)	653, 1906
HpyCH4IV	ACGT	9 cut(s)	176, 509, 557, 693, 822, 944, 1085, 1526, 1701
HpyF3I	CTNAG	7 cut(s)	300, 501, 786, 863, 884, 1322, 1883
HpySE526I	ACGT	9 cut(s)	176, 509, 557, 693, 822, 944, 1085, 1526, 1701
Hsp92I	GRCGYC	2 cut(s)	791, 1526
HspAI	GCGC	4 cut(s)	166, 889, 891, 1409
KspAI	GTTAAC	1 cut(s)	1479
Kzo9I	GATC	8 cut(s)	185, 483, 960, 1311, 1471, 1615, 1765, 1947
LmnI	GCTCC	6 cut(s)	352, 832, 838, 1049, 1443, 1805
LweI	GCATC	5 cut(s)	616, 985, 1007, 1599, 2026
MabI	ACCWGGT	1 cut(s)	762
MaeI	CTAG	3 cut(s)	735, 1349, 1482
MaeII	ACGT	9 cut(s)	176, 509, 557, 693, 822, 944, 1085, 1526, 1701
MaeIII	GTNAC	3 cut(s)	659, 766, 1444
MalI	GATC	8 cut(s)	187, 485, 962, 1313, 1473, 1617, 1767, 1949
MbiI	CCGCTC	2 cut(s)	642, 1800
MboI	GATC	8 cut(s)	185, 483, 960, 1311, 1471, 1615, 1765, 1947
MfeI	CAATTG	1 cut(s)	1734
MflI	RGATCY	4 cut(s)	185, 1311, 1615, 1765
MhlI	GDGCHC	2 cut(s)	843, 1362
MlyI	GAGTC	2 cut(s)	526, 1263
MmeI	TCCRAC	4 cut(s)	273, 1071, 1986, 2011
Mph1103I	ATGCAT	2 cut(s)	1000, 1935
MroXI	GAANNNNTTC	1 cut(s)	1646
MseI	TTAA	4 cut(s)	86, 450, 647, 1478
MslI	CAYNNNNRTG	4 cut(s)	1016, 1214, 1379, 1862
MspA1I	CMGCKG	2 cut(s)	195, 566
MspI	CCGG	5 cut(s)	38, 716, 1224, 1454, 2002
MspR9I	CCNGG	5 cut(s)	263, 764, 1442, 1454, 1894
MunI	CAATTG	1 cut(s)	1734
Mva1269I	GAATGC	2 cut(s)	2045, 2093
MvaI	CCWGG	4 cut(s)	263, 764, 1442, 1894
MvnI	CGCG	2 cut(s)	796, 891
NciI	CCSGG	1 cut(s)	1454
NcoI	CCATGG	1 cut(s)	1863
NdeII	GATC	8 cut(s)	185, 483, 960, 1311, 1471, 1615, 1765, 1947
NlaIV	GGNNCC	6 cut(s)	36, 828, 1291, 1386, 1574, 1836
NmuCI	GTSAC	3 cut(s)	659, 766, 1444
NsiI	ATGCAT	2 cut(s)	1000, 1935
NspI	RCATGY	1 cut(s)	1213
PagI	TCATGA	1 cut(s)	963
PauI	GCGCGC	1 cut(s)	889
PceI	AGGCCT	1 cut(s)	739
PcsI	WCGNNNNNNNCGW	3 cut(s)	279, 1557, 1958
PctI	GAATGC	2 cut(s)	2045, 2093
PdmI	GAANNNNTTC	1 cut(s)	1646
PfeI	GAWTC	3 cut(s)	775, 1194, 1807
PfoI	TCCNGGA	1 cut(s)	1452
PleI	GAGTC	2 cut(s)	526, 1262
PpsI	GAGTC	2 cut(s)	526, 1262
PsiI	TTATAA	1 cut(s)	1632
Psp124BI	GAGCTC	1 cut(s)	843
Psp1406I	AACGTT	1 cut(s)	509
Psp6I	CCWGG	4 cut(s)	261, 762, 1440, 1892
PspFI	CCCAGC	2 cut(s)	219, 1721
PspGI	CCWGG	4 cut(s)	261, 762, 1440, 1892
PspN4I	GGNNCC	6 cut(s)	36, 828, 1291, 1386, 1574, 1836
PspPI	GGNCC	4 cut(s)	760, 1384, 1572, 1834
PstI	CTGCAG	2 cut(s)	580, 1902
PstNI	CAGNNNCTG	1 cut(s)	355
PsuI	RGATCY	4 cut(s)	185, 1311, 1615, 1765
PteI	GCGCGC	1 cut(s)	889
PvuII	CAGCTG	1 cut(s)	566
RsaI	GTAC	5 cut(s)	182, 362, 615, 1034, 2039
RsaNI	GTAC	5 cut(s)	181, 361, 614, 1033, 2038
RseI	CAYNNNNRTG	4 cut(s)	1016, 1214, 1379, 1862
SacI	GAGCTC	1 cut(s)	843
SaqAI	TTAA	4 cut(s)	86, 450, 647, 1478
Sau3AI	GATC	8 cut(s)	185, 483, 960, 1311, 1471, 1615, 1765, 1947
Sau96I	GGNCC	4 cut(s)	760, 1384, 1572, 1834
ScaI	AGTACT	1 cut(s)	362
SchI	GAGTC	2 cut(s)	526, 1263
ScrFI	CCNGG	5 cut(s)	263, 764, 1442, 1454, 1894
SduI	GDGCHC	2 cut(s)	843, 1362
SexAI	ACCWGGT	1 cut(s)	762
SfaNI	GCATC	5 cut(s)	616, 985, 1007, 1599, 2026
SfcI	CTRYAG	3 cut(s)	561, 576, 1898
SinI	GGWCC	4 cut(s)	760, 1384, 1572, 1834
SmiMI	CAYNNNNRTG	4 cut(s)	1016, 1214, 1379, 1862
SmlI	CTYRAG	3 cut(s)	724, 842, 1135
SmoI	CTYRAG	3 cut(s)	724, 842, 1135
SseBI	AGGCCT	1 cut(s)	739
SspI	AATATT	1 cut(s)	1646
SspMI	CTAG	3 cut(s)	735, 1349, 1482
SstI	GAGCTC	1 cut(s)	843
StuI	AGGCCT	1 cut(s)	739
StyD4I	CCNGG	5 cut(s)	261, 762, 1440, 1452, 1892
StyI	CCWWGG	2 cut(s)	1036, 1863
TaaI	ACNGT	2 cut(s)	653, 1906
TaiI	ACGT	9 cut(s)	179, 512, 560, 696, 825, 947, 1088, 1529, 1704
TaqI	TCGA	4 cut(s)	413, 1103, 1810, 1981
TatI	WGTACW	3 cut(s)	180, 360, 2037
TauI	GCSGC	6 cut(s)	137, 140, 332, 796, 1246, 1800
TfiI	GAWTC	3 cut(s)	775, 1194, 1807
Tru1I	TTAA	4 cut(s)	86, 450, 647, 1478
Tru9I	TTAA	4 cut(s)	86, 450, 647, 1478
TscAI	CASTG	6 cut(s)	598, 753, 1592, 1924, 2061, 2067
TseFI	GTSAC	3 cut(s)	659, 766, 1444
Tsp45I	GTSAC	3 cut(s)	659, 766, 1444
TspGWI	ACGGA	2 cut(s)	271, 851
TspRI	CASTG	6 cut(s)	598, 753, 1592, 1924, 2061, 2067
VpaK11BI	GGWCC	4 cut(s)	760, 1384, 1572, 1834
XapI	RAATTY	7 cut(s)	17, 93, 902, 931, 1536, 1713, 1912
XceI	RCATGY	1 cut(s)	1213
XmiI	GTMKAC	2 cut(s)	560, 1851
XmnI	GAANNNNTTC	1 cut(s)	1646
XspI	CTAG	3 cut(s)	735, 1349, 1482
ZraI	GACGTC	1 cut(s)	1527
ZrmI	AGTACT	1 cut(s)	362
Zsp2I	ATGCAT	2 cut(s)	1000, 1935

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.