Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr00

Orthogroup ID

OG0001425

Physical Location & Seq

Genomic Coordinates Forward (+)

16188231 .. 16191412

Gene Structure 3182 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD00G1081000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2508 bp

ATGAAGTTCGGGAAGGAATTTTCTGCACAAATGGTGCCGGAATGGAAAGACGCATACATGGATTACGACTATCTAAAATCCCTCTTAAAAGAAATTCAAGTCTGCAAGCAAAGAAACAACCCCGACCCACAGGCCGCCGCCTCGCCCCGCAGCTACGGCCTAAAGCGCAGGTTCACGTTGTACAGATCCTTCAGCGGTCTGACAAAAAGTAGGCACACCCAGCAGCCCGCCAGCCCGTCTTCCACCTCCGACATTGAGAGCCAGGCCATTCTCGTCAACTCCGTCAACGAAAACGGCTCTGAGAACTACCAGACGATGTTTCTGATGGCGGCGGAGGAAGGCGGAGTTCAGGAGCTGGAGTACTTCAAGAGGCTTGACGACGAGTTCAATAAAGTGGACAAGTTTTATAGGTCGAAGGTTGACGAGGTGATGAAGGAAGCTGCGGTTCTTAACAAGCAAATGGACGCTTTGATTGCGTTTCGGATCAAAGTGGAGAACCCTCAGAGAACGTTTGATTGGTCAGGGGGGATGACTCGCCTTGCTTCCGATGTTGCCACGTCTACAGCTGCATTGGCTGCAGCCACCCCATGCAGTGCCAGAGCAAGCAGGCGGGTACTGGCTATGGATGCGATTGAAGAGAGCGGATTAAACAGTTCGGGTGACGAAGAAAAAGAAGACAAAAGGAGTGGAGACGTTGAGATGAAGGTGAAGAAGCCGGAGAGCTTGAGAGGCACTAGGCCTACGCCACTGGATATACTGGACCAGGTGACAATGAATCACATGGCTGAGACGCCGCGTTCCACCATCAAAGGCTTCCTCAACGTGGCTCCACATACGGAGCTCAAGTTCAGCAGGGCTAATCTCAGCAAAGTTGAACAACAGCTCAGGCGCGCTTTCATTGAATTTTACCAGAAACTTCGGCTTCTGAAGAGTTACGGATTTTTGAATACGTTGGCTTTCTCAAAGATCATGAAGAAATACGATAAGGTGGCTTCAAGAGATGCATCAAAATCTTACATGAACATGGTGGATAACTCGTACCTTGGCAGCTCCGACGAGATTACCAAGCTTATGGAAAGGGTTGAAACTACGTTCATCAAGCATTTCTCGAACTCCAATCGCCGAAAAGGAATGGCGGTCTTGAGGCCAAAACCGAGGGTAGAAAGACATCGGACAACATTCGCTATGGGTAAGGTCTCTAGCTTAATAAGTATGGTAAAATTTCTTTCACTGAACAACGACAATTTCGGCATCAATTTCTTTCGTCTGAATCTGTTTACTCAAAAACCTTCAGGTTGCTTTGCTGGCTGCACAGCTGCCCTCATATTAGCCCTTGTATTGATCATTCGTGCTCGAAATATTATGGATAATCCGGAGAGAAGTAAATACATGGAAAACATGTTTCCTCTATACAGCTTGTTCGGATTCATAGTTCTCCACATGCTTATGTATGCCGGAAATATATACTTTTGGCGGCGGTTCAGAGTCAATTACTCTTTCATATTCGGTTTCAAGCAAGGAACCGAGTTGGGATACAGAGAGATCCTCCTTCTGAGTTCTGGTCTGGCAATGTTAGCACTAGCCTCTGTGCTCGCAAATCTTGACATGGAGATGGACCCGAAAACCAATGATTACAAAGCGCTAACCGAGCTTCTCCCACTCTTCTTTGCTCCTGGTAAGATCATATTTTCACTTGTGATTGTGATATTATTATGCCCCTTCAACATCATATATCGCTCGAGTCGCTACTTCTTCCTGGTTTGTGTGTTCCACTGCATCTGTGCTCCTCTCTATAAGGTCACACTCCCGGATTTCTTCTTGGCAGATCAGTTAACTAGCCAGGTGCAAGCCTTTAGAAGTCTGCCATTCTACATTTGCTATTATGGCAGGGGAGACTTCAAAGTCAGAGAAACAACTTGCACATCAGATGACGTCTTCAAAATTTTTACTTTCATCGTGGCGTGCGTTCCATATTGGTCCCGCCTCCTTCAGTGCATCCGCCGTTTGGTTGAAGAGAAAGATCCAATGCAAGGTTATAATGGACTGAAATATTTCTTCACCATCGTAGCTGTCAGCATGAGAATAGCCTACACTCATAACAAAGACGTGAACTGGAAAATTCTTGCTGGGATTTTCTCAATTGTTGCAGCCATCTATGGAACATACTGGGATCTTGTCGTGGACTGGGGACTTCTGCAACGCCGCTCCAAGAATCGATGGTTGAGAGACAAACTCTTGGTCCCTTACAAAAGCGTATACTTCATAGCCATGGTGTTGAATGTGCTGCTGAGATTTGCCTGGCTGCAGACAGTTTTGAATTTCAGTGTCTCCTTCATGCATACACAGACAATGATCTCGGTTGTGGCGAGCTTGGAGATAATTCGTCGAGGGATATGGAGTTTTTTCCGGTTGGAGAACGAGCATCTCAACAATGTCGGAAAGTACAGAGCATTCAAGTCAGTGCCACTGCCCTTCAACTATGATGAAGACGAATGCAAAGATGAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0005802 GO:0005886 GO:0008150 GO:0012505 GO:0016020 GO:0031984 GO:0042592 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0048878 GO:0050801 GO:0055062 GO:0055065 GO:0055069 GO:0055076 GO:0055080 GO:0055081 GO:0055083 GO:0065007 GO:0065008 GO:0071944 GO:0072505 GO:0072506 GO:0072507 GO:0098771 GO:0098791

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

EXS SPX

Protein Analysis

836

Amino Acids

96.25

Weight (kDa)

9.36

Isoelectric Point (pI)

42.8

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SPX	PF03105	1 - 343	3.1e-86	SPX domain
EXS	PF03124	467 - 809	2.3e-99	EXS family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001425)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G14040 AT1G26730 AT1G26730 AT1G35350 AT1G35350 AT2G03240 AT2G03260 AT2G03260
fragaria_vesca	FvH4_4g21230 FvH4_4g30900 FvH4_4g30930 FvH4_4g30930 FvH4_4g30930 FvH4_4g30930 FvH4_4g30930 FvH4_4g30930 FvH4_4g30930 FvH4_4g30930 FvH4_4g30930 FvH4_4g30930 FvH4_4g30930 FvH4_4g30930 FvH4_4g30930 FvH4_4g30930
malus_domestica	MD00G1081000.v1.1 MD00G1081300.v1.1 MD00G1200800.v1.1 MD16G1068000.v1.1
prunus_persica	Prupe.1G278700_v2.0.a1 Prupe.1G278700_v2.0.a1 Prupe.1G278700_v2.0.a1 Prupe.1G278800_v2.0.a1
pyrus_communis	pycom13g06110 pycom13g06120 pycom16g05960
rosa_chinensis	RchiOBHm_Chr4g0439231
rosa_laevigata	RLG00000006286
rosa_multiflora	Rmu_sc0006598.1_g000012 Rmu_sc0007941.1_g000004 Rmu_ssc0000171.1_g000025
rosa_roxburghii	Rroxscaffold_2G00115520 Rroxscaffold_2G00115990 Rroxscaffold_5G00380220
rosa_rugosa	Rorug04G0317100 Rorug04G0317200
rosa_samantha	Rh4AG367700 Rh4BG379600 Rh4CG393900 Rh4DG373900
rosa_wichuraiana	Rw0G005620 Rw0G011630 Rw5G028550

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2037
AasI	GACNNNNNNGTC	1 cut(s)	1901
AatII	GACGTC	1 cut(s)	1935
Acc36I	ACCTGC	1 cut(s)	159
AccB1I	GGYRCC	1 cut(s)	34
AccBSI	CCGCTC	2 cut(s)	642, 2205
AccI	GTMKAC	2 cut(s)	560, 2256
AccII	CGCG	2 cut(s)	796, 891
AccIII	TCCGGA	1 cut(s)	1372
AclI	AACGTT	1 cut(s)	509
AclWI	GGATC	5 cut(s)	180, 491, 1537, 2015, 2178
AcsI	RAATTY	7 cut(s)	17, 93, 902, 1220, 1941, 2118, 2317
AcuI	CTGAAG	4 cut(s)	175, 947, 1275, 1973
AcyI	GRCGYC	2 cut(s)	791, 1932
AfaI	GTAC	5 cut(s)	182, 362, 615, 1040, 2444
AfeI	AGCGCT	1 cut(s)	1641
AfiI	CCNNNNNNNGG	3 cut(s)	823, 2005, 2030
AflIII	ACRYGT	1 cut(s)	1398
AjiI	CACGTC	2 cut(s)	558, 2107
AjnI	CCWGG	6 cut(s)	261, 762, 1672, 1755, 1839, 2297
AjuI	GAANNNNNNNTTGG	2 cut(s)	499, 531
AloI	GAACNNNNNNTCC	4 cut(s)	830, 862, 1416, 1448
Alw21I	GWGCWC	4 cut(s)	843, 1354, 1593, 1786
Alw26I	GTCTC	6 cut(s)	684, 782, 1201, 1887, 2220, 2332
AlwI	GGATC	5 cut(s)	180, 491, 1537, 2015, 2178
AlwNI	CAGNNNCTG	1 cut(s)	355
Ama87I	CYCGRG	1 cut(s)	1738
Aor13HI	TCCGGA	1 cut(s)	1372
Aor51HI	AGCGCT	1 cut(s)	1641
AoxI	GGCC	5 cut(s)	132, 157, 264, 737, 1145
ApoI	RAATTY	7 cut(s)	17, 93, 902, 1220, 1941, 2118, 2317
ArsI	GACNNNNNNTTYG	2 cut(s)	2223, 2255
Asp700I	GAANNNNTTC	1 cut(s)	2051
AspLEI	GCGC	4 cut(s)	168, 891, 893, 1642
AspS9I	GGNCC	4 cut(s)	760, 1615, 1977, 2239
AsuC2I	CCSGG	1 cut(s)	1808
AsuHPI	GGTGA	5 cut(s)	439, 671, 718, 778, 2050
AvaI	CYCGRG	1 cut(s)	1738
AvaII	GGWCC	4 cut(s)	760, 1615, 1977, 2239
BanI	GGYRCC	1 cut(s)	34
BanII	GRGCYC	1 cut(s)	843
BarI	GAAGNNNNNNTAC	2 cut(s)	173, 205
BbsI	GAAGAC	4 cut(s)	231, 681, 1927, 2493
Bbv12I	GWGCWC	4 cut(s)	843, 1354, 1593, 1786
BccI	CCATC	6 cut(s)	319, 812, 1606, 2069, 2159, 2211
BceAI	ACGGC	3 cut(s)	172, 310, 1986
BciT130I	CCWGG	6 cut(s)	263, 764, 1674, 1757, 1841, 2299
BciVI	GTATCC	1 cut(s)	1526
BclI	TGATCA	1 cut(s)	1341
BcnI	CCSGG	1 cut(s)	1808
BcoDI	GTCTC	6 cut(s)	684, 782, 1201, 1887, 2220, 2332
BfaI	CTAG	4 cut(s)	735, 1200, 1580, 1836
BfmI	CTRYAG	3 cut(s)	561, 576, 2303
BfoI	RGCGCY	1 cut(s)	1643
BfuAI	ACCTGC	1 cut(s)	159
BfuI	GTATCC	1 cut(s)	1526
BmcAI	AGTACT	1 cut(s)	362
Bme1390I	CCNGG	7 cut(s)	263, 764, 1674, 1757, 1808, 1841, 2299
Bme18I	GGWCC	4 cut(s)	760, 1615, 1977, 2239
BmeT110I	CYCGRG	1 cut(s)	1738
BmgBI	CACGTC	2 cut(s)	558, 2107
BmgT120I	GGNCC	4 cut(s)	760, 1615, 1977, 2239
BmiI	GGNNCC	6 cut(s)	36, 828, 1522, 1617, 1979, 2241
BmrFI	CCNGG	7 cut(s)	263, 764, 1674, 1757, 1808, 1841, 2299
BmrI	ACTGGG	2 cut(s)	2176, 2194
BmsI	GCATC	7 cut(s)	616, 991, 1013, 1260, 1785, 2004, 2431
BmuI	ACTGGG	2 cut(s)	2176, 2194
BpiI	GAAGAC	4 cut(s)	231, 681, 1927, 2493
BplI	GAGNNNNNCTC	4 cut(s)	1530, 1562, 2217, 2249
BpmI	CTGGAG	1 cut(s)	377
Bpu10I	CCTNAGC	1 cut(s)	884
BpuEI	CTTGAG	3 cut(s)	745, 827, 1162
BpuMI	CCSGG	1 cut(s)	1808
Bsa29I	ATCGAT	1 cut(s)	2215
BsaHI	GRCGYC	2 cut(s)	791, 1932
BsaI	GGTCTC	1 cut(s)	1201
BsaJI	CCNNGG	3 cut(s)	1042, 1154, 2268
BsaWI	WCCGGW	2 cut(s)	1372, 2406
BsaXI	ACNNNNNCTCC	4 cut(s)	344, 374, 830, 860
Bsc4I	CCNNNNNNNGG	3 cut(s)	823, 2005, 2030
Bse1I	ACTGG	6 cut(s)	621, 753, 762, 2117, 2171, 2189
Bse3DI	GCAATG	1 cut(s)	1575
BseAI	TCCGGA	1 cut(s)	1372
BseBI	CCWGG	6 cut(s)	263, 764, 1674, 1757, 1841, 2299
BseCI	ATCGAT	1 cut(s)	2215
BseDI	CCNNGG	3 cut(s)	1042, 1154, 2268
BseGI	GGATG	3 cut(s)	534, 631, 1995
BseLI	CCNNNNNNNGG	3 cut(s)	823, 2005, 2030
BseMI	GCAATG	1 cut(s)	1575
BseMII	CTCAG	7 cut(s)	291, 515, 777, 877, 898, 1544, 2279
BseNI	ACTGG	6 cut(s)	621, 753, 762, 2117, 2171, 2189
BsePI	GCGCGC	1 cut(s)	889
BseRI	GAGGAG	1 cut(s)	1776
BseYI	CCCAGC	2 cut(s)	219, 2126
BsgI	GTGCAG	2 cut(s)	9, 1294
Bsh1236I	CGCG	2 cut(s)	796, 891
BshFI	GGCC	5 cut(s)	134, 159, 266, 739, 1147
BshNI	GGYRCC	1 cut(s)	34
BshVI	ATCGAT	1 cut(s)	2215
BsiHKAI	GWGCWC	4 cut(s)	843, 1354, 1593, 1786
BsiHKCI	CYCGRG	1 cut(s)	1738
BsiSI	CCGG	6 cut(s)	38, 716, 1373, 1455, 1808, 2407
BslFI	GGGAC	3 cut(s)	1963, 2202, 2225
BslI	CCNNNNNNNGG	3 cut(s)	823, 2005, 2030
BsmAI	GTCTC	6 cut(s)	684, 782, 1201, 1887, 2220, 2332
BsmBI	CGTCTC	2 cut(s)	684, 782
BsmFI	GGGAC	3 cut(s)	1963, 2202, 2225
BsmI	GAATGC	2 cut(s)	2450, 2498
BsnI	GGCC	5 cut(s)	134, 159, 266, 739, 1147
Bso31I	GGTCTC	1 cut(s)	1201
BsoBI	CYCGRG	1 cut(s)	1738
Bsp1286I	GDGCHC	4 cut(s)	843, 1354, 1593, 1786
Bsp13I	TCCGGA	1 cut(s)	1372
Bsp1407I	TGTACA	1 cut(s)	180
Bsp19I	CCATGG	1 cut(s)	2268
BspANI	GGCC	5 cut(s)	134, 159, 266, 739, 1147
BspCNI	CTCAG	7 cut(s)	292, 514, 778, 876, 897, 1545, 2280
BspDI	ATCGAT	1 cut(s)	2215
BspEI	TCCGGA	1 cut(s)	1372
BspFNI	CGCG	2 cut(s)	796, 891
BspHI	TCATGA	1 cut(s)	969
BspLI	GGNNCC	6 cut(s)	36, 828, 1522, 1617, 1979, 2241
BspMAI	CTGCAG	2 cut(s)	580, 2307
BspMI	ACCTGC	1 cut(s)	159
BspPI	GGATC	5 cut(s)	180, 491, 1537, 2015, 2178
BspT107I	GGYRCC	1 cut(s)	34
BspTNI	GGTCTC	1 cut(s)	1201
BsrBI	CCGCTC	2 cut(s)	642, 2205
BsrDI	GCAATG	1 cut(s)	1575
BsrGI	TGTACA	1 cut(s)	180
BsrI	ACTGG	6 cut(s)	621, 753, 762, 2117, 2171, 2189
BssECI	CCNNGG	3 cut(s)	1042, 1154, 2268
BssHII	GCGCGC	1 cut(s)	889
BssNAI	GTATAC	1 cut(s)	2257
BssNI	GRCGYC	2 cut(s)	791, 1932
BssT1I	CCWWGG	2 cut(s)	1042, 2268
Bst1107I	GTATAC	1 cut(s)	2257
Bst2UI	CCWGG	6 cut(s)	263, 764, 1674, 1757, 1841, 2299
Bst4CI	ACNGT	2 cut(s)	653, 2311
Bst6I	CTCTTC	4 cut(s)	630, 923, 1667, 2007
BstACI	GRCGYC	2 cut(s)	791, 1932
BstAPI	GCANNNNNTGC	1 cut(s)	575
BstAUI	TGTACA	1 cut(s)	180
BstDEI	CTNAG	7 cut(s)	300, 501, 786, 863, 884, 1553, 2288
BstDSI	CCRYGG	1 cut(s)	2268
BstF5I	GGATG	3 cut(s)	534, 631, 1995
BstFNI	CGCG	2 cut(s)	796, 891
BstH2I	RGCGCY	1 cut(s)	1643
BstHHI	GCGC	4 cut(s)	168, 891, 893, 1642
BstMAI	GTCTC	6 cut(s)	684, 782, 1201, 1887, 2220, 2332
BstNI	CCWGG	6 cut(s)	263, 764, 1674, 1757, 1841, 2299
BstNSI	RCATGY	2 cut(s)	1402, 1444
BstSCI	CCNGG	7 cut(s)	261, 762, 1672, 1755, 1806, 1839, 2297
BstSFI	CTRYAG	3 cut(s)	561, 576, 2303
BstUI	CGCG	2 cut(s)	796, 891
BstV2I	GAAGAC	4 cut(s)	231, 681, 1927, 2493
BstX2I	RGATCY	4 cut(s)	185, 1542, 2020, 2170
BstXI	CCANNNNNNTGG	1 cut(s)	1072
BstYI	RGATCY	4 cut(s)	185, 1542, 2020, 2170
BstZ17I	GTATAC	1 cut(s)	2257
Bsu15I	ATCGAT	1 cut(s)	2215
BsuI	GTATCC	1 cut(s)	1526
BsuRI	GGCC	5 cut(s)	134, 159, 266, 739, 1147
BsuTUI	ATCGAT	1 cut(s)	2215
BtgI	CCRYGG	1 cut(s)	2268
BtrI	CACGTC	2 cut(s)	558, 2107
BtsCI	GGATG	3 cut(s)	534, 631, 1995
BtsI	GCAGTG	3 cut(s)	598, 1771, 2465
BtsIMutI	CAGTG	8 cut(s)	598, 746, 1229, 1771, 1997, 2329, 2465, 2466
BveI	ACCTGC	1 cut(s)	159
CaiI	CAGNNNCTG	1 cut(s)	355
CciI	TCATGA	1 cut(s)	969
CfoI	GCGC	4 cut(s)	168, 891, 893, 1642
Cfr13I	GGNCC	4 cut(s)	760, 1615, 1977, 2239
ClaI	ATCGAT	1 cut(s)	2215
CseI	GACGC	3 cut(s)	59, 473, 799
CsiI	ACCWGGT	1 cut(s)	762
Csp6I	GTAC	5 cut(s)	181, 361, 614, 1039, 2443
CspCI	CAANNNNNGTGG	2 cut(s)	667, 702
CviQI	GTAC	5 cut(s)	181, 361, 614, 1039, 2443
DdeI	CTNAG	7 cut(s)	300, 501, 786, 863, 884, 1553, 2288
DrdI	GACNNNNNNGTC	1 cut(s)	1901
DseDI	GACNNNNNNGTC	1 cut(s)	1901
Eam1104I	CTCTTC	4 cut(s)	630, 923, 1667, 2007
EarI	CTCTTC	4 cut(s)	630, 923, 1667, 2007
EciI	GGCGGA	3 cut(s)	347, 357, 1988
Ecl136II	GAGCTC	1 cut(s)	841
Eco130I	CCWWGG	2 cut(s)	1042, 2268
Eco147I	AGGCCT	1 cut(s)	739
Eco24I	GRGCYC	1 cut(s)	843
Eco31I	GGTCTC	1 cut(s)	1201
Eco47I	GGWCC	4 cut(s)	760, 1615, 1977, 2239
Eco47III	AGCGCT	1 cut(s)	1641
Eco53kI	GAGCTC	1 cut(s)	841
Eco57I	CTGAAG	4 cut(s)	175, 947, 1275, 1973
Eco88I	CYCGRG	1 cut(s)	1738
EcoICRI	GAGCTC	1 cut(s)	841
EcoRII	CCWGG	6 cut(s)	261, 762, 1672, 1755, 1839, 2297
EcoT14I	CCWWGG	2 cut(s)	1042, 2268
EcoT22I	ATGCAT	2 cut(s)	1006, 2340
EcoT38I	GRGCYC	1 cut(s)	843
ErhI	CCWWGG	2 cut(s)	1042, 2268
Esp3I	CGTCTC	2 cut(s)	684, 782
FaqI	GGGAC	3 cut(s)	1963, 2202, 2225
FauI	CCCGC	4 cut(s)	155, 235, 603, 1988
FbaI	TGATCA	1 cut(s)	1341
FblI	GTMKAC	2 cut(s)	560, 2256
FokI	GGATG	3 cut(s)	541, 638, 1982
FriOI	GRGCYC	1 cut(s)	843
FspBI	CTAG	4 cut(s)	735, 1200, 1580, 1836
GlaI	GCGC	4 cut(s)	167, 890, 892, 1641
GsaI	CCCAGC	2 cut(s)	223, 2130
GsuI	CTGGAG	1 cut(s)	377
HaeII	RGCGCY	1 cut(s)	1643
HaeIII	GGCC	5 cut(s)	134, 159, 266, 739, 1147
HapII	CCGG	6 cut(s)	38, 716, 1373, 1455, 1808, 2407
HgaI	GACGC	3 cut(s)	59, 473, 799
HhaI	GCGC	4 cut(s)	168, 891, 893, 1642
Hin1I	GRCGYC	2 cut(s)	791, 1932
Hin6I	GCGC	4 cut(s)	166, 889, 891, 1640
HinP1I	GCGC	4 cut(s)	166, 889, 891, 1640
HincII	GTYRAC	4 cut(s)	277, 286, 421, 1833
HindII	GTYRAC	4 cut(s)	277, 286, 421, 1833
HindIII	AAGCTT	1 cut(s)	1067
HinfI	GANTC	7 cut(s)	532, 775, 1270, 1425, 1485, 1741, 2212
HpaI	GTTAAC	1 cut(s)	1833
HpaII	CCGG	6 cut(s)	38, 716, 1373, 1455, 1808, 2407
HphI	GGTGA	5 cut(s)	439, 671, 718, 778, 2050
Hpy188III	TCNNGA	9 cut(s)	10, 350, 367, 970, 996, 1108, 1141, 1373, 1601
Hpy99I	CGWCG	3 cut(s)	383, 1058, 2388
HpyCH4III	ACNGT	2 cut(s)	653, 2311
HpyCH4IV	ACGT	9 cut(s)	176, 509, 557, 693, 822, 950, 1091, 1932, 2106
HpyF3I	CTNAG	7 cut(s)	300, 501, 786, 863, 884, 1553, 2288
HpySE526I	ACGT	9 cut(s)	176, 509, 557, 693, 822, 950, 1091, 1932, 2106
Hsp92I	GRCGYC	2 cut(s)	791, 1932
HspAI	GCGC	4 cut(s)	166, 889, 891, 1640
Kpn2I	TCCGGA	1 cut(s)	1372
Ksp22I	TGATCA	1 cut(s)	1341
KspAI	GTTAAC	1 cut(s)	1833
LmnI	GCTCC	7 cut(s)	352, 832, 838, 1055, 1675, 1789, 2210
LweI	GCATC	7 cut(s)	616, 991, 1013, 1260, 1785, 2004, 2431
MabI	ACCWGGT	1 cut(s)	762
MaeI	CTAG	4 cut(s)	735, 1200, 1580, 1836
MaeII	ACGT	9 cut(s)	176, 509, 557, 693, 822, 950, 1091, 1932, 2106
MaeIII	GTNAC	4 cut(s)	659, 766, 932, 1798
MbiI	CCGCTC	2 cut(s)	642, 2205
MfeI	CAATTG	1 cut(s)	2139
MflI	RGATCY	4 cut(s)	185, 1542, 2020, 2170
MhlI	GDGCHC	4 cut(s)	843, 1354, 1593, 1786
MlyI	GAGTC	3 cut(s)	526, 1494, 1750
MmeI	TCCRAC	4 cut(s)	273, 1077, 2391, 2416
Mph1103I	ATGCAT	2 cut(s)	1006, 2340
MroI	TCCGGA	1 cut(s)	1372
MroXI	GAANNNNTTC	1 cut(s)	2051
MseI	TTAA	5 cut(s)	86, 450, 647, 1205, 1832
MslI	CAYNNNNRTG	4 cut(s)	1022, 1445, 1610, 2267
MspA1I	CMGCKG	3 cut(s)	195, 566, 1316
MspI	CCGG	6 cut(s)	38, 716, 1373, 1455, 1808, 2407
MspR9I	CCNGG	7 cut(s)	263, 764, 1674, 1757, 1808, 1841, 2299
MunI	CAATTG	1 cut(s)	2139
Mva1269I	GAATGC	2 cut(s)	2450, 2498
MvaI	CCWGG	6 cut(s)	263, 764, 1674, 1757, 1841, 2299
MvnI	CGCG	2 cut(s)	796, 891
NciI	CCSGG	1 cut(s)	1808
NcoI	CCATGG	1 cut(s)	2268
NlaIV	GGNNCC	6 cut(s)	36, 828, 1522, 1617, 1979, 2241
NmuCI	GTSAC	3 cut(s)	659, 766, 1798
NsiI	ATGCAT	2 cut(s)	1006, 2340
NspI	RCATGY	2 cut(s)	1402, 1444
PaeR7I	CTCGAG	1 cut(s)	1738
PagI	TCATGA	1 cut(s)	969
PauI	GCGCGC	1 cut(s)	889
PceI	AGGCCT	1 cut(s)	739
PciI	ACATGT	1 cut(s)	1398
PcsI	WCGNNNNNNNCGW	3 cut(s)	279, 1962, 2363
PctI	GAATGC	2 cut(s)	2450, 2498
PdmI	GAANNNNTTC	1 cut(s)	2051
PfeI	GAWTC	4 cut(s)	775, 1270, 1425, 2212
PfoI	TCCNGGA	1 cut(s)	1806
PleI	GAGTC	3 cut(s)	526, 1493, 1749
PpsI	GAGTC	3 cut(s)	526, 1493, 1749
PscI	ACATGT	1 cut(s)	1398
PsiI	TTATAA	1 cut(s)	2037
Psp124BI	GAGCTC	1 cut(s)	843
Psp1406I	AACGTT	1 cut(s)	509
Psp6I	CCWGG	6 cut(s)	261, 762, 1672, 1755, 1839, 2297
PspFI	CCCAGC	2 cut(s)	219, 2126
PspGI	CCWGG	6 cut(s)	261, 762, 1672, 1755, 1839, 2297
PspN4I	GGNNCC	6 cut(s)	36, 828, 1522, 1617, 1979, 2241
PspPI	GGNCC	4 cut(s)	760, 1615, 1977, 2239
PspXI	VCTCGAGB	1 cut(s)	1738
PstI	CTGCAG	2 cut(s)	580, 2307
PstNI	CAGNNNCTG	1 cut(s)	355
PsuI	RGATCY	4 cut(s)	185, 1542, 2020, 2170
PteI	GCGCGC	1 cut(s)	889
PvuII	CAGCTG	2 cut(s)	566, 1316
RsaI	GTAC	5 cut(s)	182, 362, 615, 1040, 2444
RsaNI	GTAC	5 cut(s)	181, 361, 614, 1039, 2443
RseI	CAYNNNNRTG	4 cut(s)	1022, 1445, 1610, 2267
SacI	GAGCTC	1 cut(s)	843
SaqAI	TTAA	5 cut(s)	86, 450, 647, 1205, 1832
Sau96I	GGNCC	4 cut(s)	760, 1615, 1977, 2239
ScaI	AGTACT	1 cut(s)	362
SchI	GAGTC	3 cut(s)	526, 1494, 1750
ScrFI	CCNGG	7 cut(s)	263, 764, 1674, 1757, 1808, 1841, 2299
SduI	GDGCHC	4 cut(s)	843, 1354, 1593, 1786
SexAI	ACCWGGT	1 cut(s)	762
SfaNI	GCATC	7 cut(s)	616, 991, 1013, 1260, 1785, 2004, 2431
SfcI	CTRYAG	3 cut(s)	561, 576, 2303
Sfr274I	CTCGAG	1 cut(s)	1738
SinI	GGWCC	4 cut(s)	760, 1615, 1977, 2239
SlaI	CTCGAG	1 cut(s)	1738
SmiMI	CAYNNNNRTG	4 cut(s)	1022, 1445, 1610, 2267
SmlI	CTYRAG	4 cut(s)	724, 842, 1141, 1738
SmoI	CTYRAG	4 cut(s)	724, 842, 1141, 1738
SseBI	AGGCCT	1 cut(s)	739
SspI	AATATT	2 cut(s)	1360, 2051
SspMI	CTAG	4 cut(s)	735, 1200, 1580, 1836
SstI	GAGCTC	1 cut(s)	843
StuI	AGGCCT	1 cut(s)	739
StyD4I	CCNGG	7 cut(s)	261, 762, 1672, 1755, 1806, 1839, 2297
StyI	CCWWGG	2 cut(s)	1042, 2268
TaaI	ACNGT	2 cut(s)	653, 2311
TaiI	ACGT	9 cut(s)	179, 512, 560, 696, 825, 953, 1094, 1935, 2109
TaqI	TCGA	6 cut(s)	413, 1109, 1354, 1739, 2215, 2386
TatI	WGTACW	3 cut(s)	180, 360, 2442
TauI	GCSGC	6 cut(s)	137, 140, 332, 796, 1477, 2205
TfiI	GAWTC	4 cut(s)	775, 1270, 1425, 2212
Tru1I	TTAA	5 cut(s)	86, 450, 647, 1205, 1832
Tru9I	TTAA	5 cut(s)	86, 450, 647, 1205, 1832
TscAI	CASTG	8 cut(s)	598, 753, 1236, 1778, 1997, 2329, 2466, 2472
TseFI	GTSAC	3 cut(s)	659, 766, 1798
Tsp45I	GTSAC	3 cut(s)	659, 766, 1798
TspGWI	ACGGA	3 cut(s)	271, 851, 951
TspRI	CASTG	8 cut(s)	598, 753, 1236, 1778, 1997, 2329, 2466, 2472
VpaK11BI	GGWCC	4 cut(s)	760, 1615, 1977, 2239
XapI	RAATTY	7 cut(s)	17, 93, 902, 1220, 1941, 2118, 2317
XceI	RCATGY	2 cut(s)	1402, 1444
XhoI	CTCGAG	1 cut(s)	1738
XmiI	GTMKAC	2 cut(s)	560, 2256
XmnI	GAANNNNTTC	1 cut(s)	2051
XspI	CTAG	4 cut(s)	735, 1200, 1580, 1836
ZraI	GACGTC	1 cut(s)	1933
ZrmI	AGTACT	1 cut(s)	362
Zsp2I	ATGCAT	2 cut(s)	1006, 2340

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.