Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr00

Orthogroup ID

OG0001425

Physical Location & Seq

Genomic Coordinates Forward (+)

16225026 .. 16228305

Gene Structure 3280 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD00G1081300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2490 bp

ATGAAGTTCGGGAAGGAATTTTCTGCACAAATGGTGCCGGAATGGAAAGACGCATACATGGATTACGACTATCTAAAATCCCTCTTAAAAGAAATTCAAGTCTGCAAGCAAAGAAACAACCCCGACCCACAGGCCGCCGCCTCGCCCCGCAGCTACGGCCTAAAGCGCAGGCTCACGTTGTACAGAGCCTTCAGCGGTCTGACCAAAAGTAGGCACACCCAGCAGCCCGCCAGCCCTTCTTCCACCTCCGACATTGAGAGCCAGGCCATTCTTGTCAATTCCGTCAACGAAAACGGCTCTAAGAGCTACCAGACGATGTTTCTGATGGCGGCGGAGGAAGGCGGAGTTCAGGAGCTGGAGTACTTCAAGAGGCTTGACGACGAGTTCAATAAAGTGGACAAGTTTTATAGGTCGAAGGTTGACGAGGTGATGAAGGAAGCTGCGGTTCTTAACAAGCAAATGGACGCTTTGATTGCGTTTCGGATCAAAGTGGAGAACCCTCAGAGAACGTTTGATTGGTCAGGGGAGATGACTCGCCTTGCTTCCGATGTTGCCACGTCTACAGCTGCATTGGCTGCAGCCACCCCACGCAGTGCCAGAGCAAGCAGGCGGGTACTGGCTATGGATGCGATTGAAGAGAGCGGATTAAACAGTTCGGGTGACGAAGAAAAAGAAGACAAAAGGAGTGGAGACGTTGAGATGAAGGTGAAGAAGCCGGAGAGCTTGAGAGGCACTAGGCCTACGCCACTGGATATACTGGACCAGGTGACAATGAATCACATGGCTGAGACGCCGCGTTCCACCATCAAAGGCTTCCTCAACGTGGCTCCACATACGGAGCTCAAGTTCAGCAGGGCTAATCTCAGCAAAGTTGAACAACAGCTCAGGCGCGCTTTCATTGAATTTTACCAGAAACTTCGGCTTCTGAAGAGCTACGGATTTTTGAATACGTTGGCTTTCTCAAAGATCATGAAGAAATACGATAAGGTGGCTTCAAGAGATGCATCAAAATCTTACATGAACATGGTGGATAACTCGTACCTTGGCAGCTCCGACGAGATTACCAAGCTTATGGAAAGGGTTGAAACTACGTTCATCAAGCATTTCTCGAACTCCAATCGCCGAAAAGGAATGGCGGTCTTGAGACCAAAACCGAGGGTAGAAAGACATCGGACAACATTCGCTCTGGGTAAGGTCTCTAGCTTAATAAGTATGGTAAAATTTCTTTCACTGAACAACGACAATTTCGGCATCAATTTCTTTCGTCTGAATCTGTTTACTCAAAAACCTTCAGGTTGCTTTGCTGGCTGCACAGCTGCCCTCATATTAGCCCTTGTATTGATCATTCGTGCTCGAAATATTATGGATAATCCGGAGAGAAGTAAATACATGGAAAACATGTTTCCTCTATACAGCTTGTTCGGATTCATAGTTCTCCACATGCTTATGTATGCCGGAAATATATACTTTTGGCGGCGGTTCAGAGTCAATTACTCTTTCATATTCGGTTTCAAGCAAGGAACCGAGTTGGGATACAGAGAGGTCCTCCTTCTGAGTTCTGGTCTGGCAATGTTAGCACTAGCCTCTGTGCTCGCAAATCTTGACATGGAGATGGACCCGAAAACCAATGATTACAAAGCGCTAACCGAGCTTCTCCCACTCTTCTTGCTCCTGCTTGTGATTGTGATATTATTATGCCCCTTCAACATCATATATCGCTCGAGTCGCTACTTCTTCCTGGTTTGTGTGTTCCACTGCATCTGTGCTCCTCTCTATAAGGTCACACTCCCGGATTTCTTCTTGGCAGATCAGTTAACTAGCCAGGTGCAAGCCTTTAGAAGTCTGCCATTCTACATTTGCTATTATGGCGGGGGAGACTACAAAGTCAGAAAAACAACTTGCAGATCAGATGACGTCTTCAAAATTTTTACTTTCATCGTGGCGTGCGTTCCATATTGGTCCCGCCTCCTTCAGTGCATCCGCCGTTTGGTTGAAGAGAAAGATCCAATGCAAGGTTACAATGGACTGAAATATTTCTTCACCATCGTAGCTGTCAGCATGAGAATAGCCTACACTCATAACAAAGACGTGAACTGGAAAATTCTTGCTGGGATTTTCTCAATTGTTGCAGCTATCTATGGAACATACTGGGATCTTGTCGTGGACTGGGGACTTCTGCAACGCCGCTCCAAGAATCGATGGTTGAGAGACAAACTCTTGGTCCCTTACAAAAGCGTATACTTCATAGCCATGGTGTTGAATGTGCTGCTGAGATTTGCCTGGCTGCAGACAGTTTTGAATTTCAGTGTCTCCTTCATGCATACACAGACAATGATCTCGGTTGTGGCGAGCTTGGAGATAATTCGTCGAGGGATATGGAGTTTTTTCCGGTTGGAGAACGAGCATCTCAACAATGTCGGAAAGTACAGAGCATTCAAGTCAGTGCCACTGCCCTTCAACTATGATGAAGACGAATGCAAAGATGAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0005802 GO:0005886 GO:0008150 GO:0012505 GO:0016020 GO:0031984 GO:0042592 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0048878 GO:0050801 GO:0055062 GO:0055065 GO:0055069 GO:0055076 GO:0055080 GO:0055081 GO:0055083 GO:0065007 GO:0065008 GO:0071944 GO:0072505 GO:0072506 GO:0072507 GO:0098771 GO:0098791

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

EXS SPX

Protein Analysis

830

Amino Acids

95.62

Weight (kDa)

9.44

Isoelectric Point (pI)

42.88

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SPX	PF03105	1 - 343	5.1e-86	SPX domain
EXS	PF03124	467 - 803	2.2e-102	EXS family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001425)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G14040 AT1G26730 AT1G26730 AT1G35350 AT1G35350 AT2G03240 AT2G03260 AT2G03260
fragaria_vesca	FvH4_4g21230 FvH4_4g30900 FvH4_4g30930 FvH4_4g30930 FvH4_4g30930 FvH4_4g30930 FvH4_4g30930 FvH4_4g30930 FvH4_4g30930 FvH4_4g30930 FvH4_4g30930 FvH4_4g30930 FvH4_4g30930 FvH4_4g30930 FvH4_4g30930 FvH4_4g30930
malus_domestica	MD00G1081000.v1.1 MD00G1081300.v1.1 MD00G1200800.v1.1 MD16G1068000.v1.1
prunus_persica	Prupe.1G278700_v2.0.a1 Prupe.1G278700_v2.0.a1 Prupe.1G278700_v2.0.a1 Prupe.1G278800_v2.0.a1
pyrus_communis	pycom13g06110 pycom13g06120 pycom16g05960
rosa_chinensis	RchiOBHm_Chr4g0439231
rosa_laevigata	RLG00000006286
rosa_multiflora	Rmu_sc0006598.1_g000012 Rmu_sc0007941.1_g000004 Rmu_ssc0000171.1_g000025
rosa_roxburghii	Rroxscaffold_2G00115520 Rroxscaffold_2G00115990 Rroxscaffold_5G00380220
rosa_rugosa	Rorug04G0317100 Rorug04G0317200
rosa_samantha	Rh4AG367700 Rh4BG379600 Rh4CG393900 Rh4DG373900
rosa_wichuraiana	Rw0G005620 Rw0G011630 Rw5G028550

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1883
AatII	GACGTC	1 cut(s)	1917
AccB1I	GGYRCC	1 cut(s)	34
AccBSI	CCGCTC	2 cut(s)	642, 2187
AccI	GTMKAC	2 cut(s)	560, 2238
AccII	CGCG	2 cut(s)	796, 891
AccIII	TCCGGA	1 cut(s)	1372
AclI	AACGTT	1 cut(s)	509
AclWI	GGATC	3 cut(s)	491, 1997, 2160
AcsI	RAATTY	7 cut(s)	17, 93, 902, 1220, 1923, 2100, 2299
AcuI	CTGAAG	4 cut(s)	175, 947, 1275, 1955
AcyI	GRCGYC	2 cut(s)	791, 1914
AdeI	CACNNNGTG	1 cut(s)	593
AfaI	GTAC	5 cut(s)	182, 362, 615, 1040, 2426
AfeI	AGCGCT	1 cut(s)	1641
AfiI	CCNNNNNNNGG	4 cut(s)	210, 823, 1987, 2012
AflIII	ACRYGT	1 cut(s)	1398
AjiI	CACGTC	2 cut(s)	558, 2089
AjnI	CCWGG	5 cut(s)	261, 762, 1737, 1821, 2279
AjuI	GAANNNNNNNTTGG	2 cut(s)	499, 531
AloI	GAACNNNNNNTCC	4 cut(s)	830, 862, 1416, 1448
Alw21I	GWGCWC	4 cut(s)	843, 1354, 1593, 1768
Alw26I	GTCTC	7 cut(s)	684, 782, 1138, 1201, 1869, 2202, 2314
AlwI	GGATC	3 cut(s)	491, 1997, 2160
AlwNI	CAGNNNCTG	1 cut(s)	355
Ama87I	CYCGRG	1 cut(s)	1720
Aor13HI	TCCGGA	1 cut(s)	1372
Aor51HI	AGCGCT	1 cut(s)	1641
AoxI	GGCC	4 cut(s)	132, 157, 264, 737
ApoI	RAATTY	7 cut(s)	17, 93, 902, 1220, 1923, 2100, 2299
ArsI	GACNNNNNNTTYG	2 cut(s)	2205, 2237
Asp700I	GAANNNNTTC	1 cut(s)	2033
AspLEI	GCGC	4 cut(s)	168, 891, 893, 1642
AspS9I	GGNCC	5 cut(s)	760, 1543, 1615, 1959, 2221
AsuC2I	CCSGG	1 cut(s)	1790
AsuHPI	GGTGA	5 cut(s)	439, 671, 718, 778, 2032
AvaI	CYCGRG	1 cut(s)	1720
AvaII	GGWCC	5 cut(s)	760, 1543, 1615, 1959, 2221
BanI	GGYRCC	1 cut(s)	34
BanII	GRGCYC	1 cut(s)	843
BarI	GAAGNNNNNNTAC	2 cut(s)	173, 205
BbsI	GAAGAC	3 cut(s)	681, 1909, 2475
Bbv12I	GWGCWC	4 cut(s)	843, 1354, 1593, 1768
BccI	CCATC	5 cut(s)	319, 812, 1606, 2051, 2193
BceAI	ACGGC	3 cut(s)	172, 310, 1968
BciT130I	CCWGG	5 cut(s)	263, 764, 1739, 1823, 2281
BciVI	GTATCC	1 cut(s)	1526
BclI	TGATCA	1 cut(s)	1341
BcnI	CCSGG	1 cut(s)	1790
BcoDI	GTCTC	7 cut(s)	684, 782, 1138, 1201, 1869, 2202, 2314
BfaI	CTAG	4 cut(s)	735, 1200, 1580, 1818
BfmI	CTRYAG	3 cut(s)	561, 576, 2285
BfoI	RGCGCY	1 cut(s)	1643
BfuI	GTATCC	1 cut(s)	1526
BmcAI	AGTACT	1 cut(s)	362
Bme1390I	CCNGG	6 cut(s)	263, 764, 1739, 1790, 1823, 2281
Bme18I	GGWCC	5 cut(s)	760, 1543, 1615, 1959, 2221
BmeT110I	CYCGRG	1 cut(s)	1720
BmgBI	CACGTC	2 cut(s)	558, 2089
BmgT120I	GGNCC	5 cut(s)	760, 1543, 1615, 1959, 2221
BmiI	GGNNCC	6 cut(s)	36, 828, 1522, 1617, 1961, 2223
BmrFI	CCNGG	6 cut(s)	263, 764, 1739, 1790, 1823, 2281
BmrI	ACTGGG	2 cut(s)	2158, 2176
BmsI	GCATC	7 cut(s)	616, 991, 1013, 1260, 1767, 1986, 2413
BmuI	ACTGGG	2 cut(s)	2158, 2176
BpiI	GAAGAC	3 cut(s)	681, 1909, 2475
BplI	GAGNNNNNCTC	4 cut(s)	1530, 1562, 2199, 2231
BpmI	CTGGAG	1 cut(s)	377
Bpu10I	CCTNAGC	1 cut(s)	884
BpuEI	CTTGAG	3 cut(s)	745, 827, 1162
BpuMI	CCSGG	1 cut(s)	1790
Bsa29I	ATCGAT	1 cut(s)	2197
BsaHI	GRCGYC	2 cut(s)	791, 1914
BsaI	GGTCTC	2 cut(s)	1138, 1201
BsaJI	CCNNGG	3 cut(s)	1042, 1154, 2250
BsaWI	WCCGGW	2 cut(s)	1372, 2388
BsaXI	ACNNNNNCTCC	4 cut(s)	344, 374, 830, 860
Bsc4I	CCNNNNNNNGG	4 cut(s)	210, 823, 1987, 2012
Bse1I	ACTGG	6 cut(s)	621, 753, 762, 2099, 2153, 2171
Bse3DI	GCAATG	1 cut(s)	1575
BseAI	TCCGGA	1 cut(s)	1372
BseBI	CCWGG	5 cut(s)	263, 764, 1739, 1823, 2281
BseCI	ATCGAT	1 cut(s)	2197
BseDI	CCNNGG	3 cut(s)	1042, 1154, 2250
BseGI	GGATG	2 cut(s)	631, 1977
BseLI	CCNNNNNNNGG	4 cut(s)	210, 823, 1987, 2012
BseMI	GCAATG	1 cut(s)	1575
BseMII	CTCAG	6 cut(s)	515, 777, 877, 898, 1544, 2261
BseNI	ACTGG	6 cut(s)	621, 753, 762, 2099, 2153, 2171
BsePI	GCGCGC	1 cut(s)	889
BseRI	GAGGAG	1 cut(s)	1758
BseYI	CCCAGC	2 cut(s)	219, 2108
BsgI	GTGCAG	2 cut(s)	9, 1294
Bsh1236I	CGCG	2 cut(s)	796, 891
BshFI	GGCC	4 cut(s)	134, 159, 266, 739
BshNI	GGYRCC	1 cut(s)	34
BshVI	ATCGAT	1 cut(s)	2197
BsiHKAI	GWGCWC	4 cut(s)	843, 1354, 1593, 1768
BsiHKCI	CYCGRG	1 cut(s)	1720
BsiSI	CCGG	6 cut(s)	38, 716, 1373, 1455, 1790, 2389
BslFI	GGGAC	3 cut(s)	1945, 2184, 2207
BslI	CCNNNNNNNGG	4 cut(s)	210, 823, 1987, 2012
BsmAI	GTCTC	7 cut(s)	684, 782, 1138, 1201, 1869, 2202, 2314
BsmBI	CGTCTC	2 cut(s)	684, 782
BsmFI	GGGAC	3 cut(s)	1945, 2184, 2207
BsmI	GAATGC	2 cut(s)	2432, 2480
BsnI	GGCC	4 cut(s)	134, 159, 266, 739
Bso31I	GGTCTC	2 cut(s)	1138, 1201
BsoBI	CYCGRG	1 cut(s)	1720
Bsp1286I	GDGCHC	4 cut(s)	843, 1354, 1593, 1768
Bsp13I	TCCGGA	1 cut(s)	1372
Bsp1407I	TGTACA	1 cut(s)	180
Bsp143I	GATC	8 cut(s)	483, 966, 1341, 1807, 1904, 2002, 2152, 2334
Bsp19I	CCATGG	1 cut(s)	2250
BspANI	GGCC	4 cut(s)	134, 159, 266, 739
BspCNI	CTCAG	6 cut(s)	514, 778, 876, 897, 1545, 2262
BspDI	ATCGAT	1 cut(s)	2197
BspEI	TCCGGA	1 cut(s)	1372
BspFNI	CGCG	2 cut(s)	796, 891
BspHI	TCATGA	1 cut(s)	969
BspLI	GGNNCC	6 cut(s)	36, 828, 1522, 1617, 1961, 2223
BspMAI	CTGCAG	2 cut(s)	580, 2289
BspPI	GGATC	3 cut(s)	491, 1997, 2160
BspQI	GCTCTTC	1 cut(s)	923
BspT107I	GGYRCC	1 cut(s)	34
BspTNI	GGTCTC	2 cut(s)	1138, 1201
BsrBI	CCGCTC	2 cut(s)	642, 2187
BsrDI	GCAATG	1 cut(s)	1575
BsrGI	TGTACA	1 cut(s)	180
BsrI	ACTGG	6 cut(s)	621, 753, 762, 2099, 2153, 2171
BssECI	CCNNGG	3 cut(s)	1042, 1154, 2250
BssHII	GCGCGC	1 cut(s)	889
BssMI	GATC	8 cut(s)	483, 966, 1341, 1807, 1904, 2002, 2152, 2334
BssNAI	GTATAC	1 cut(s)	2239
BssNI	GRCGYC	2 cut(s)	791, 1914
BssT1I	CCWWGG	2 cut(s)	1042, 2250
Bst1107I	GTATAC	1 cut(s)	2239
Bst2UI	CCWGG	5 cut(s)	263, 764, 1739, 1823, 2281
Bst4CI	ACNGT	2 cut(s)	653, 2293
Bst6I	CTCTTC	4 cut(s)	630, 923, 1667, 1989
BstACI	GRCGYC	2 cut(s)	791, 1914
BstAPI	GCANNNNNTGC	1 cut(s)	575
BstAUI	TGTACA	1 cut(s)	180
BstDEI	CTNAG	7 cut(s)	300, 501, 786, 863, 884, 1553, 2270
BstDSI	CCRYGG	1 cut(s)	2250
BstF5I	GGATG	2 cut(s)	631, 1977
BstFNI	CGCG	2 cut(s)	796, 891
BstH2I	RGCGCY	1 cut(s)	1643
BstHHI	GCGC	4 cut(s)	168, 891, 893, 1642
BstKTI	GATC	8 cut(s)	486, 969, 1344, 1810, 1907, 2005, 2155, 2337
BstMAI	GTCTC	7 cut(s)	684, 782, 1138, 1201, 1869, 2202, 2314
BstMBI	GATC	8 cut(s)	483, 966, 1341, 1807, 1904, 2002, 2152, 2334
BstNI	CCWGG	5 cut(s)	263, 764, 1739, 1823, 2281
BstNSI	RCATGY	2 cut(s)	1402, 1444
BstSCI	CCNGG	6 cut(s)	261, 762, 1737, 1788, 1821, 2279
BstSFI	CTRYAG	3 cut(s)	561, 576, 2285
BstUI	CGCG	2 cut(s)	796, 891
BstV2I	GAAGAC	3 cut(s)	681, 1909, 2475
BstX2I	RGATCY	2 cut(s)	2002, 2152
BstXI	CCANNNNNNTGG	1 cut(s)	1072
BstYI	RGATCY	2 cut(s)	2002, 2152
BstZ17I	GTATAC	1 cut(s)	2239
Bsu15I	ATCGAT	1 cut(s)	2197
BsuI	GTATCC	1 cut(s)	1526
BsuRI	GGCC	4 cut(s)	134, 159, 266, 739
BsuTUI	ATCGAT	1 cut(s)	2197
BtgI	CCRYGG	1 cut(s)	2250
BtrI	CACGTC	2 cut(s)	558, 2089
BtsCI	GGATG	2 cut(s)	631, 1977
BtsI	GCAGTG	3 cut(s)	598, 1753, 2447
BtsIMutI	CAGTG	8 cut(s)	598, 746, 1229, 1753, 1979, 2311, 2447, 2448
CaiI	CAGNNNCTG	1 cut(s)	355
CciI	TCATGA	1 cut(s)	969
CfoI	GCGC	4 cut(s)	168, 891, 893, 1642
Cfr13I	GGNCC	5 cut(s)	760, 1543, 1615, 1959, 2221
ClaI	ATCGAT	1 cut(s)	2197
CseI	GACGC	3 cut(s)	59, 473, 799
CsiI	ACCWGGT	1 cut(s)	762
Csp6I	GTAC	5 cut(s)	181, 361, 614, 1039, 2425
CspCI	CAANNNNNGTGG	4 cut(s)	667, 702, 1647, 1682
CviQI	GTAC	5 cut(s)	181, 361, 614, 1039, 2425
DdeI	CTNAG	7 cut(s)	300, 501, 786, 863, 884, 1553, 2270
DpnI	GATC	8 cut(s)	485, 968, 1343, 1809, 1906, 2004, 2154, 2336
DpnII	GATC	8 cut(s)	483, 966, 1341, 1807, 1904, 2002, 2152, 2334
DraIII	CACNNNGTG	1 cut(s)	593
DrdI	GACNNNNNNGTC	1 cut(s)	1883
DseDI	GACNNNNNNGTC	1 cut(s)	1883
Eam1104I	CTCTTC	4 cut(s)	630, 923, 1667, 1989
EarI	CTCTTC	4 cut(s)	630, 923, 1667, 1989
EciI	GGCGGA	3 cut(s)	347, 357, 1970
Ecl136II	GAGCTC	1 cut(s)	841
Eco130I	CCWWGG	2 cut(s)	1042, 2250
Eco147I	AGGCCT	1 cut(s)	739
Eco24I	GRGCYC	1 cut(s)	843
Eco31I	GGTCTC	2 cut(s)	1138, 1201
Eco47I	GGWCC	5 cut(s)	760, 1543, 1615, 1959, 2221
Eco47III	AGCGCT	1 cut(s)	1641
Eco53kI	GAGCTC	1 cut(s)	841
Eco57I	CTGAAG	4 cut(s)	175, 947, 1275, 1955
Eco88I	CYCGRG	1 cut(s)	1720
EcoICRI	GAGCTC	1 cut(s)	841
EcoO109I	RGGNCCY	1 cut(s)	1543
EcoRII	CCWGG	5 cut(s)	261, 762, 1737, 1821, 2279
EcoT14I	CCWWGG	2 cut(s)	1042, 2250
EcoT22I	ATGCAT	2 cut(s)	1006, 2322
EcoT38I	GRGCYC	1 cut(s)	843
ErhI	CCWWGG	2 cut(s)	1042, 2250
Esp3I	CGTCTC	2 cut(s)	684, 782
FaqI	GGGAC	3 cut(s)	1945, 2184, 2207
FauI	CCCGC	5 cut(s)	155, 235, 603, 1862, 1970
FbaI	TGATCA	1 cut(s)	1341
FblI	GTMKAC	2 cut(s)	560, 2238
FokI	GGATG	2 cut(s)	638, 1964
FriOI	GRGCYC	1 cut(s)	843
FspBI	CTAG	4 cut(s)	735, 1200, 1580, 1818
GlaI	GCGC	4 cut(s)	167, 890, 892, 1641
GsaI	CCCAGC	2 cut(s)	223, 2112
GsuI	CTGGAG	1 cut(s)	377
HaeII	RGCGCY	1 cut(s)	1643
HaeIII	GGCC	4 cut(s)	134, 159, 266, 739
HapII	CCGG	6 cut(s)	38, 716, 1373, 1455, 1790, 2389
HgaI	GACGC	3 cut(s)	59, 473, 799
HhaI	GCGC	4 cut(s)	168, 891, 893, 1642
Hin1I	GRCGYC	2 cut(s)	791, 1914
Hin6I	GCGC	4 cut(s)	166, 889, 891, 1640
HinP1I	GCGC	4 cut(s)	166, 889, 891, 1640
HincII	GTYRAC	3 cut(s)	286, 421, 1815
HindII	GTYRAC	3 cut(s)	286, 421, 1815
HindIII	AAGCTT	1 cut(s)	1067
HinfI	GANTC	7 cut(s)	532, 775, 1270, 1425, 1485, 1723, 2194
HpaI	GTTAAC	1 cut(s)	1815
HpaII	CCGG	6 cut(s)	38, 716, 1373, 1455, 1790, 2389
HphI	GGTGA	5 cut(s)	439, 671, 718, 778, 2032
Hpy166II	GTNNAC	9 cut(s)	286, 397, 421, 561, 1278, 1815, 2092, 2164, 2239
Hpy188III	TCNNGA	9 cut(s)	10, 350, 367, 970, 996, 1108, 1141, 1373, 1601
Hpy8I	GTNNAC	9 cut(s)	286, 397, 421, 561, 1278, 1815, 2092, 2164, 2239
Hpy99I	CGWCG	3 cut(s)	383, 1058, 2370
HpyCH4III	ACNGT	2 cut(s)	653, 2293
HpyCH4IV	ACGT	9 cut(s)	176, 509, 557, 693, 822, 950, 1091, 1914, 2088
HpyF3I	CTNAG	7 cut(s)	300, 501, 786, 863, 884, 1553, 2270
HpySE526I	ACGT	9 cut(s)	176, 509, 557, 693, 822, 950, 1091, 1914, 2088
Hsp92I	GRCGYC	2 cut(s)	791, 1914
HspAI	GCGC	4 cut(s)	166, 889, 891, 1640
Kpn2I	TCCGGA	1 cut(s)	1372
Ksp22I	TGATCA	1 cut(s)	1341
KspAI	GTTAAC	1 cut(s)	1815
Kzo9I	GATC	8 cut(s)	483, 966, 1341, 1807, 1904, 2002, 2152, 2334
LguI	GCTCTTC	1 cut(s)	923
LmnI	GCTCC	7 cut(s)	352, 832, 838, 1055, 1674, 1771, 2192
LweI	GCATC	7 cut(s)	616, 991, 1013, 1260, 1767, 1986, 2413
MabI	ACCWGGT	1 cut(s)	762
MaeI	CTAG	4 cut(s)	735, 1200, 1580, 1818
MaeII	ACGT	9 cut(s)	176, 509, 557, 693, 822, 950, 1091, 1914, 2088
MaeIII	GTNAC	4 cut(s)	659, 766, 1780, 2015
MalI	GATC	8 cut(s)	485, 968, 1343, 1809, 1906, 2004, 2154, 2336
MbiI	CCGCTC	2 cut(s)	642, 2187
MboI	GATC	8 cut(s)	483, 966, 1341, 1807, 1904, 2002, 2152, 2334
MfeI	CAATTG	1 cut(s)	2121
MflI	RGATCY	2 cut(s)	2002, 2152
MhlI	GDGCHC	4 cut(s)	843, 1354, 1593, 1768
MlyI	GAGTC	3 cut(s)	526, 1494, 1732
MmeI	TCCRAC	4 cut(s)	273, 1077, 2373, 2398
Mph1103I	ATGCAT	2 cut(s)	1006, 2322
MroI	TCCGGA	1 cut(s)	1372
MroXI	GAANNNNTTC	1 cut(s)	2033
MseI	TTAA	5 cut(s)	86, 450, 647, 1205, 1814
MslI	CAYNNNNRTG	4 cut(s)	1022, 1445, 1610, 2249
MspA1I	CMGCKG	3 cut(s)	195, 566, 1316
MspI	CCGG	6 cut(s)	38, 716, 1373, 1455, 1790, 2389
MspR9I	CCNGG	6 cut(s)	263, 764, 1739, 1790, 1823, 2281
MunI	CAATTG	1 cut(s)	2121
Mva1269I	GAATGC	2 cut(s)	2432, 2480
MvaI	CCWGG	5 cut(s)	263, 764, 1739, 1823, 2281
MvnI	CGCG	2 cut(s)	796, 891
NciI	CCSGG	1 cut(s)	1790
NcoI	CCATGG	1 cut(s)	2250
NdeII	GATC	8 cut(s)	483, 966, 1341, 1807, 1904, 2002, 2152, 2334
NlaIV	GGNNCC	6 cut(s)	36, 828, 1522, 1617, 1961, 2223
NmuCI	GTSAC	3 cut(s)	659, 766, 1780
NsiI	ATGCAT	2 cut(s)	1006, 2322
NspI	RCATGY	2 cut(s)	1402, 1444
PaeR7I	CTCGAG	1 cut(s)	1720
PagI	TCATGA	1 cut(s)	969
PauI	GCGCGC	1 cut(s)	889
PceI	AGGCCT	1 cut(s)	739
PciI	ACATGT	1 cut(s)	1398
PciSI	GCTCTTC	1 cut(s)	923
PcsI	WCGNNNNNNNCGW	2 cut(s)	1944, 2345
PctI	GAATGC	2 cut(s)	2432, 2480
PdmI	GAANNNNTTC	1 cut(s)	2033
PfeI	GAWTC	4 cut(s)	775, 1270, 1425, 2194
PfoI	TCCNGGA	1 cut(s)	1788
PleI	GAGTC	3 cut(s)	526, 1493, 1731
PpsI	GAGTC	3 cut(s)	526, 1493, 1731
PpuMI	RGGWCCY	1 cut(s)	1543
PscI	ACATGT	1 cut(s)	1398
Psp124BI	GAGCTC	1 cut(s)	843
Psp1406I	AACGTT	1 cut(s)	509
Psp5II	RGGWCCY	1 cut(s)	1543
Psp6I	CCWGG	5 cut(s)	261, 762, 1737, 1821, 2279
PspFI	CCCAGC	2 cut(s)	219, 2108
PspGI	CCWGG	5 cut(s)	261, 762, 1737, 1821, 2279
PspN4I	GGNNCC	6 cut(s)	36, 828, 1522, 1617, 1961, 2223
PspPI	GGNCC	5 cut(s)	760, 1543, 1615, 1959, 2221
PspPPI	RGGWCCY	1 cut(s)	1543
PspXI	VCTCGAGB	1 cut(s)	1720
PstI	CTGCAG	2 cut(s)	580, 2289
PstNI	CAGNNNCTG	1 cut(s)	355
PsuI	RGATCY	2 cut(s)	2002, 2152
PteI	GCGCGC	1 cut(s)	889
PvuII	CAGCTG	2 cut(s)	566, 1316
RsaI	GTAC	5 cut(s)	182, 362, 615, 1040, 2426
RsaNI	GTAC	5 cut(s)	181, 361, 614, 1039, 2425
RseI	CAYNNNNRTG	4 cut(s)	1022, 1445, 1610, 2249
SacI	GAGCTC	1 cut(s)	843
SapI	GCTCTTC	1 cut(s)	923
SaqAI	TTAA	5 cut(s)	86, 450, 647, 1205, 1814
Sau3AI	GATC	8 cut(s)	483, 966, 1341, 1807, 1904, 2002, 2152, 2334
Sau96I	GGNCC	5 cut(s)	760, 1543, 1615, 1959, 2221
ScaI	AGTACT	1 cut(s)	362
SchI	GAGTC	3 cut(s)	526, 1494, 1732
ScrFI	CCNGG	6 cut(s)	263, 764, 1739, 1790, 1823, 2281
SduI	GDGCHC	4 cut(s)	843, 1354, 1593, 1768
SexAI	ACCWGGT	1 cut(s)	762
SfaNI	GCATC	7 cut(s)	616, 991, 1013, 1260, 1767, 1986, 2413
SfcI	CTRYAG	3 cut(s)	561, 576, 2285
Sfr274I	CTCGAG	1 cut(s)	1720
SinI	GGWCC	5 cut(s)	760, 1543, 1615, 1959, 2221
SlaI	CTCGAG	1 cut(s)	1720
SmiMI	CAYNNNNRTG	4 cut(s)	1022, 1445, 1610, 2249
SmlI	CTYRAG	4 cut(s)	724, 842, 1141, 1720
SmoI	CTYRAG	4 cut(s)	724, 842, 1141, 1720
SseBI	AGGCCT	1 cut(s)	739
SspI	AATATT	2 cut(s)	1360, 2033
SspMI	CTAG	4 cut(s)	735, 1200, 1580, 1818
SstI	GAGCTC	1 cut(s)	843
StuI	AGGCCT	1 cut(s)	739
StyD4I	CCNGG	6 cut(s)	261, 762, 1737, 1788, 1821, 2279
StyI	CCWWGG	2 cut(s)	1042, 2250
TaaI	ACNGT	2 cut(s)	653, 2293
TaiI	ACGT	9 cut(s)	179, 512, 560, 696, 825, 953, 1094, 1917, 2091
TaqI	TCGA	6 cut(s)	413, 1109, 1354, 1721, 2197, 2368
TatI	WGTACW	3 cut(s)	180, 360, 2424
TauI	GCSGC	6 cut(s)	137, 140, 332, 796, 1477, 2187
TfiI	GAWTC	4 cut(s)	775, 1270, 1425, 2194
Tru1I	TTAA	5 cut(s)	86, 450, 647, 1205, 1814
Tru9I	TTAA	5 cut(s)	86, 450, 647, 1205, 1814
TscAI	CASTG	8 cut(s)	598, 753, 1236, 1760, 1979, 2311, 2448, 2454
TseFI	GTSAC	3 cut(s)	659, 766, 1780
Tsp45I	GTSAC	3 cut(s)	659, 766, 1780
TspGWI	ACGGA	3 cut(s)	271, 851, 951
TspRI	CASTG	8 cut(s)	598, 753, 1236, 1760, 1979, 2311, 2448, 2454
VpaK11BI	GGWCC	5 cut(s)	760, 1543, 1615, 1959, 2221
XapI	RAATTY	7 cut(s)	17, 93, 902, 1220, 1923, 2100, 2299
XceI	RCATGY	2 cut(s)	1402, 1444
XhoI	CTCGAG	1 cut(s)	1720
XmiI	GTMKAC	2 cut(s)	560, 2238
XmnI	GAANNNNTTC	1 cut(s)	2033
XspI	CTAG	4 cut(s)	735, 1200, 1580, 1818
ZraI	GACGTC	1 cut(s)	1915
ZrmI	AGTACT	1 cut(s)	362
Zsp2I	ATGCAT	2 cut(s)	1006, 2322

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.