AT2G04570

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0008949
Physical Location & Seq
Forward (+)
1594588 .. 1596372
1785 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G04570.1

Sequence Viewer

Length: 1053 bp
ATGGGACACCTCAAAAGTCTCTTCACAATTCTTTTCCTAATAGCTATGTCTTCAACCGTAACATTTGCCGGTAAAATACCCGCGATCATCGTGTTTGGCGACTCTTCCGTTGATGCCGGGAACAACAACTATATCCCAACTGTTGCTAGAAGCAACTTTGAGCCATATGGACGGGACTTTGTTGGTGGAAAGCCGACCGGACGGTTTTGTAACGGAAAGATTGCGACGGATTTTATGTCCGAAGCCTTAGGGCTTAAACCAATCATTCCGGCGTACTTGGATCCTTCTTATAACATTTCGGATTTTGCAACAGGTGTTACCTTTGCTTCTGCTGCCACTGGCTATGACAACGCCACTTCTGATGTTCTGTCGGTACTACCTCTATGGAAACAACTCGAATACTACAAAGAATACCAAACAAAACTTAAAGCATACCAAGGAAAAGACAGAGGCACAGAGACTATAGAAAGCTCTCTCTACCTCATAAGCATAGGGACCAACGATTTCCTCGAGAATTACTTTGCCTTTCCGGGCCGTTCTTCGCAATATTCCGTCAGTCTTTACCAAGATTTTCTAGCCGGAATCGCGAAAGAATTTGTGAAGAAGTTGCATGGACTTGGTGCTAGAAAGATCTCACTAGGTGGGTTACCTCCAATGGGATGCATGCCTTTAGAGAGAGCCACCAACATTGGCACTGGAGGTGAGTGCGTAGGACGGTACAACGACATAGCCGTCCAGTTCAACAGCAAGCTTGATAAGATGGTTGAGAAGCTGAGCAAAGAGCTCCCTGGTTCCAACCTCGTTTTCTCAAATCCCTATGAGCCATTTATGCGGATCATCAAGAACCCTTCCTCCTTTGGGTTCGAGGTGGTGGGAGCGGCATGTTGCGCGACAGGGATGTTCGAGATGGGATATGGTTGTCAAAGGAATAACCCATTCACATGTACAAACGCAGACAAGTATGTGTTTTGGGACTCATTTCACCCAACACAGAAGACTAATCACATCATGGCCAATGCTCTCATGAACAGCACATTCCCTCACTTCCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

350

Amino Acids

38.48

Weight (kDa)

7.55

Isoelectric Point (pI)

28.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 29 - 341 1.4e-33 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 291
AasI GACNNNNNNGTC 1 cut(s) 731
AccBSI CCGCTC 1 cut(s) 878
AccII CGCG 3 cut(s) 83, 587, 890
AciI CCGC 3 cut(s) 81, 832, 878
AclWI GGATC 3 cut(s) 275, 288, 842
AcoI YGGCCR 1 cut(s) 1011
AcsI RAATTY 1 cut(s) 593
AdeI CACNNNGTG 1 cut(s) 641
AfaI GTAC 4 cut(s) 275, 375, 719, 946
AfiI CCNNNNNNNGG 2 cut(s) 656, 858
AflIII ACRYGT 1 cut(s) 941
AgsI TTSAA 2 cut(s) 54, 742
AjnI CCWGG 1 cut(s) 787
AjuI GAANNNNNNNTTGG 2 cut(s) 788, 820
AloI GAACNNNNNNTCC 2 cut(s) 836, 868
AluBI AGCT 5 cut(s) 44, 471, 751, 772, 784
AluI AGCT 5 cut(s) 44, 471, 751, 772, 784
Alw21I GWGCWC 1 cut(s) 786
Alw26I GTCTC 2 cut(s) 23, 452
AlwI GGATC 3 cut(s) 275, 288, 842
Ama87I CYCGRG 1 cut(s) 509
AoxI GGCC 2 cut(s) 532, 1011
ApeKI GCWGC 1 cut(s) 332
ApoI RAATTY 1 cut(s) 593
AspLEI GCGC 1 cut(s) 890
AspS9I GGNCC 2 cut(s) 495, 532
AsuC2I CCSGG 2 cut(s) 118, 531
AsuHPI GGTGA 2 cut(s) 713, 974
AvaI CYCGRG 1 cut(s) 509
AvaII GGWCC 1 cut(s) 495
AxyI CCTNAGG 1 cut(s) 247
BalI TGGCCA 1 cut(s) 1013
BamHI GGATCC 1 cut(s) 280
BanII GRGCYC 1 cut(s) 786
BbsI GAAGAC 2 cut(s) 42, 1001
Bbv12I GWGCWC 1 cut(s) 786
BbvI GCAGC 1 cut(s) 319
BccI CCATC 2 cut(s) 754, 901
BceAI ACGGC 2 cut(s) 519, 716
BciT130I CCWGG 1 cut(s) 789
BcnI CCSGG 2 cut(s) 118, 531
BcoDI GTCTC 2 cut(s) 23, 452
BfaI CTAG 4 cut(s) 147, 575, 624, 638
BfmI CTRYAG 1 cut(s) 462
BglII AGATCT 1 cut(s) 630
BisI GCNGC 2 cut(s) 333, 879
BlpI GCTNAGC 1 cut(s) 773
BlsI GCNGC 2 cut(s) 334, 880
Bme1390I CCNGG 3 cut(s) 118, 531, 789
Bme18I GGWCC 1 cut(s) 495
BmeT110I CYCGRG 1 cut(s) 509
BmgT120I GGNCC 2 cut(s) 495, 532
BmiI GGNNCC 3 cut(s) 282, 496, 793
BmrFI CCNGG 3 cut(s) 118, 531, 789
BmsI GCATC 2 cut(s) 103, 650
BpiI GAAGAC 2 cut(s) 42, 1001
BpmI CTGGAG 1 cut(s) 717
Bpu1102I GCTNAGC 1 cut(s) 773
BpuMI CCSGG 2 cut(s) 118, 531
BsaJI CCNNGG 2 cut(s) 436, 787
BsaWI WCCGGW 1 cut(s) 197
BsaXI ACNNNNNCTCC 2 cut(s) 836, 866
Bsc4I CCNNNNNNNGG 2 cut(s) 656, 858
Bse118I RCCGGY 1 cut(s) 68
Bse1I ACTGG 3 cut(s) 343, 700, 736
Bse21I CCTNAGG 1 cut(s) 247
BseBI CCWGG 1 cut(s) 789
BseDI CCNNGG 2 cut(s) 436, 787
BseGI GGATG 2 cut(s) 665, 903
BseLI CCNNNNNNNGG 2 cut(s) 656, 858
BseMII CTCAG 1 cut(s) 764
BseNI ACTGG 3 cut(s) 343, 700, 736
BseXI GCAGC 1 cut(s) 319
Bsh1236I CGCG 3 cut(s) 83, 587, 890
Bsh1285I CGRYCG 1 cut(s) 198
BshFI GGCC 2 cut(s) 534, 1013
BsiEI CGRYCG 1 cut(s) 198
BsiHKAI GWGCWC 1 cut(s) 786
BsiHKCI CYCGRG 1 cut(s) 509
BsiSI CCGG 6 cut(s) 69, 117, 198, 269, 530, 579
BslFI GGGAC 4 cut(s) 18, 188, 508, 986
BslI CCNNNNNNNGG 2 cut(s) 656, 858
BsmAI GTCTC 2 cut(s) 23, 452
BsmFI GGGAC 4 cut(s) 18, 188, 508, 986
BsnI GGCC 2 cut(s) 534, 1013
BsoBI CYCGRG 1 cut(s) 509
Bsp1286I GDGCHC 1 cut(s) 786
Bsp1407I TGTACA 1 cut(s) 944
Bsp143I GATC 4 cut(s) 84, 280, 630, 834
Bsp1720I GCTNAGC 1 cut(s) 773
Bsp68I TCGCGA 1 cut(s) 587
BspACI CCGC 3 cut(s) 81, 832, 878
BspANI GGCC 2 cut(s) 534, 1013
BspCNI CTCAG 1 cut(s) 765
BspFNI CGCG 3 cut(s) 83, 587, 890
BspHI TCATGA 1 cut(s) 1023
BspLI GGNNCC 3 cut(s) 282, 496, 793
BspPI GGATC 3 cut(s) 275, 288, 842
BsrBI CCGCTC 1 cut(s) 878
BsrFI RCCGGY 1 cut(s) 68
BsrGI TGTACA 1 cut(s) 944
BsrI ACTGG 3 cut(s) 343, 700, 736
BssAI RCCGGY 1 cut(s) 68
BssECI CCNNGG 2 cut(s) 436, 787
BssMI GATC 4 cut(s) 84, 280, 630, 834
BssT1I CCWWGG 1 cut(s) 436
Bst2UI CCWGG 1 cut(s) 789
Bst4CI ACNGT 4 cut(s) 58, 142, 204, 717
Bst6I CTCTTC 2 cut(s) 26, 109
BstAUI TGTACA 1 cut(s) 944
BstC8I GCNNGC 2 cut(s) 665, 749
BstDEI CTNAG 2 cut(s) 247, 773
BstEII GGTNACC 1 cut(s) 645
BstF5I GGATG 2 cut(s) 665, 903
BstFNI CGCG 3 cut(s) 83, 587, 890
BstHHI GCGC 1 cut(s) 890
BstKTI GATC 4 cut(s) 87, 283, 633, 837
BstMAI GTCTC 2 cut(s) 23, 452
BstMBI GATC 4 cut(s) 84, 280, 630, 834
BstMCI CGRYCG 1 cut(s) 198
BstMWI GCNNNNNNNGC 4 cut(s) 332, 584, 829, 887
BstNI CCWGG 1 cut(s) 789
BstNSI RCATGY 3 cut(s) 667, 885, 945
BstPI GGTNACC 1 cut(s) 645
BstSCI CCNGG 3 cut(s) 116, 529, 787
BstSFI CTRYAG 1 cut(s) 462
BstUI CGCG 3 cut(s) 83, 587, 890
BstV1I GCAGC 1 cut(s) 319
BstV2I GAAGAC 2 cut(s) 42, 1001
BstX2I RGATCY 2 cut(s) 280, 630
BstYI RGATCY 2 cut(s) 280, 630
Bsu36I CCTNAGG 1 cut(s) 247
BsuRI GGCC 2 cut(s) 534, 1013
BtsCI GGATG 2 cut(s) 665, 903
BtsIMutI CAGTG 2 cut(s) 336, 693
BtuMI TCGCGA 1 cut(s) 587
Cac8I GCNNGC 2 cut(s) 665, 749
CciI TCATGA 1 cut(s) 1023
CfoI GCGC 1 cut(s) 890
Cfr10I RCCGGY 1 cut(s) 68
Cfr13I GGNCC 2 cut(s) 495, 532
Csp6I GTAC 4 cut(s) 274, 374, 718, 945
CspCI CAANNNNNGTGG 2 cut(s) 670, 705
CviAII CATG 6 cut(s) 611, 664, 882, 942, 1009, 1024
CviQI GTAC 4 cut(s) 274, 374, 718, 945
DdeI CTNAG 2 cut(s) 247, 773
DpnI GATC 4 cut(s) 86, 282, 632, 836
DpnII GATC 4 cut(s) 84, 280, 630, 834
DraIII CACNNNGTG 1 cut(s) 641
DrdI GACNNNNNNGTC 1 cut(s) 731
DseDI GACNNNNNNGTC 1 cut(s) 731
EaeI YGGCCR 1 cut(s) 1011
Eam1104I CTCTTC 2 cut(s) 26, 109
EarI CTCTTC 2 cut(s) 26, 109
Ecl136II GAGCTC 1 cut(s) 784
Eco130I CCWWGG 1 cut(s) 436
Eco24I GRGCYC 1 cut(s) 786
Eco47I GGWCC 1 cut(s) 495
Eco53kI GAGCTC 1 cut(s) 784
Eco81I CCTNAGG 1 cut(s) 247
Eco88I CYCGRG 1 cut(s) 509
Eco91I GGTNACC 1 cut(s) 645
EcoICRI GAGCTC 1 cut(s) 784
EcoO65I GGTNACC 1 cut(s) 645
EcoRII CCWGG 1 cut(s) 787
EcoT14I CCWWGG 1 cut(s) 436
EcoT22I ATGCAT 1 cut(s) 665
EcoT38I GRGCYC 1 cut(s) 786
ErhI CCWWGG 1 cut(s) 436
FaeI CATG 6 cut(s) 614, 667, 885, 945, 1012, 1027
FaqI GGGAC 4 cut(s) 18, 188, 508, 986
FatI CATG 6 cut(s) 610, 663, 881, 941, 1008, 1023
FauI CCCGC 1 cut(s) 88
FauNDI CATATG 1 cut(s) 166
Fnu4HI GCNGC 2 cut(s) 333, 879
FokI GGATG 2 cut(s) 672, 910
FriOI GRGCYC 1 cut(s) 786
Fsp4HI GCNGC 2 cut(s) 333, 879
FspBI CTAG 4 cut(s) 147, 575, 624, 638
GlaI GCGC 1 cut(s) 889
GluI GCNGC 2 cut(s) 333, 879
GsuI CTGGAG 1 cut(s) 717
HaeIII GGCC 2 cut(s) 534, 1013
HapII CCGG 6 cut(s) 69, 117, 198, 269, 530, 579
HhaI GCGC 1 cut(s) 890
Hin1II CATG 6 cut(s) 614, 667, 885, 945, 1012, 1027
Hin6I GCGC 1 cut(s) 888
HinP1I GCGC 1 cut(s) 888
HindIII AAGCTT 1 cut(s) 749
HinfI GANTC 3 cut(s) 101, 582, 974
HpaII CCGG 6 cut(s) 69, 117, 198, 269, 530, 579
HphI GGTGA 2 cut(s) 713, 974
Hpy188I TCNGA 3 cut(s) 241, 301, 361
Hpy188III TCNNGA 5 cut(s) 511, 586, 841, 904, 1024
Hpy99I CGWCG 1 cut(s) 229
HpyAV CCTTC 2 cut(s) 294, 858
HpyCH4III ACNGT 4 cut(s) 58, 142, 204, 717
HpyCH4V TGCA 3 cut(s) 308, 610, 663
HpyF10VI GCNNNNNNNGC 4 cut(s) 332, 584, 829, 887
HpyF3I CTNAG 2 cut(s) 247, 773
Hsp92II CATG 6 cut(s) 614, 667, 885, 945, 1012, 1027
HspAI GCGC 1 cut(s) 888
Kzo9I GATC 4 cut(s) 84, 280, 630, 834
LmnI GCTCC 2 cut(s) 789, 875
Lsp1109I GCAGC 1 cut(s) 319
LweI GCATC 2 cut(s) 103, 650
MaeI CTAG 4 cut(s) 147, 575, 624, 638
MaeIII GTNAC 4 cut(s) 58, 209, 316, 645
MalI GATC 4 cut(s) 86, 282, 632, 836
MbiI CCGCTC 1 cut(s) 878
MboI GATC 4 cut(s) 84, 280, 630, 834
MboII GAAGA 6 cut(s) 13, 42, 96, 531, 613, 1006
MflI RGATCY 2 cut(s) 280, 630
MhlI GDGCHC 1 cut(s) 786
MlsI TGGCCA 1 cut(s) 1013
MluCI AATT 3 cut(s) 27, 514, 593
MluNI TGGCCA 1 cut(s) 1013
MlyI GAGTC 2 cut(s) 95, 968
MmeI TCCRAC 1 cut(s) 819
Mox20I TGGCCA 1 cut(s) 1013
Mph1103I ATGCAT 1 cut(s) 665
MscI TGGCCA 1 cut(s) 1013
MseI TTAA 2 cut(s) 255, 426
MslI CAYNNNNRTG 1 cut(s) 940
Msp20I TGGCCA 1 cut(s) 1013
MspI CCGG 6 cut(s) 69, 117, 198, 269, 530, 579
MspR9I CCNGG 3 cut(s) 118, 531, 789
MvaI CCWGG 1 cut(s) 789
MvnI CGCG 3 cut(s) 83, 587, 890
MwoI GCNNNNNNNGC 4 cut(s) 332, 584, 829, 887
NciI CCSGG 2 cut(s) 118, 531
NdeI CATATG 1 cut(s) 166
NdeII GATC 4 cut(s) 84, 280, 630, 834
NlaIII CATG 6 cut(s) 614, 667, 885, 945, 1012, 1027
NlaIV GGNNCC 3 cut(s) 282, 496, 793
NruI TCGCGA 1 cut(s) 587
NsiI ATGCAT 1 cut(s) 665
NspI RCATGY 3 cut(s) 667, 885, 945
PaeI GCATGC 1 cut(s) 667
PaeR7I CTCGAG 1 cut(s) 509
PagI TCATGA 1 cut(s) 1023
PciI ACATGT 1 cut(s) 941
PcsI WCGNNNNNNNCGW 3 cut(s) 96, 507, 729
PfeI GAWTC 1 cut(s) 582
PkrI GCNGC 2 cut(s) 334, 880
PleI GAGTC 2 cut(s) 95, 968
PpsI GAGTC 2 cut(s) 95, 968
PscI ACATGT 1 cut(s) 941
PsiI TTATAA 1 cut(s) 291
Psp124BI GAGCTC 1 cut(s) 786
Psp6I CCWGG 1 cut(s) 787
PspEI GGTNACC 1 cut(s) 645
PspGI CCWGG 1 cut(s) 787
PspN4I GGNNCC 3 cut(s) 282, 496, 793
PspPI GGNCC 2 cut(s) 495, 532
PsuI RGATCY 2 cut(s) 280, 630
RruI TCGCGA 1 cut(s) 587
RsaI GTAC 4 cut(s) 275, 375, 719, 946
RsaNI GTAC 4 cut(s) 274, 374, 718, 945
RseI CAYNNNNRTG 1 cut(s) 940
SacI GAGCTC 1 cut(s) 786
SaqAI TTAA 2 cut(s) 255, 426
SatI GCNGC 2 cut(s) 333, 879
Sau3AI GATC 4 cut(s) 84, 280, 630, 834
Sau96I GGNCC 2 cut(s) 495, 532
SchI GAGTC 2 cut(s) 95, 968
ScrFI CCNGG 3 cut(s) 118, 531, 789
SduI GDGCHC 1 cut(s) 786
SfaNI GCATC 2 cut(s) 103, 650
SfcI CTRYAG 1 cut(s) 462
Sfr274I CTCGAG 1 cut(s) 509
SinI GGWCC 1 cut(s) 495
SlaI CTCGAG 1 cut(s) 509
SmiMI CAYNNNNRTG 1 cut(s) 940
SmlI CTYRAG 1 cut(s) 509
SmoI CTYRAG 1 cut(s) 509
SphI GCATGC 1 cut(s) 667
Sse9I AATT 3 cut(s) 27, 514, 593
SsiI CCGC 3 cut(s) 81, 832, 878
SspI AATATT 1 cut(s) 548
SspMI CTAG 4 cut(s) 147, 575, 624, 638
SstI GAGCTC 1 cut(s) 786
StyD4I CCNGG 3 cut(s) 116, 529, 787
StyI CCWWGG 1 cut(s) 436
TaaI ACNGT 4 cut(s) 58, 142, 204, 717
TaqI TCGA 4 cut(s) 396, 510, 864, 903
TasI AATT 3 cut(s) 27, 514, 593
TatI WGTACW 1 cut(s) 944
TauI GCSGC 1 cut(s) 881
TfiI GAWTC 1 cut(s) 582
Tru1I TTAA 2 cut(s) 255, 426
Tru9I TTAA 2 cut(s) 255, 426
TscAI CASTG 2 cut(s) 343, 700
TseI GCWGC 1 cut(s) 332
TspDTI ATGAA 1 cut(s) 1040
TspGWI ACGGA 4 cut(s) 97, 228, 242, 541
TspRI CASTG 2 cut(s) 343, 700
VpaK11BI GGWCC 1 cut(s) 495
XapI RAATTY 1 cut(s) 593
XceI RCATGY 3 cut(s) 667, 885, 945
XhoI CTCGAG 1 cut(s) 509
XspI CTAG 4 cut(s) 147, 575, 624, 638
Zsp2I ATGCAT 1 cut(s) 665
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.