MD09G1116500.v1.1

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0008949
Physical Location & Seq
Reverse (-)
8956001 .. 8958890
2890 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1116500.v1.1.491

Sequence Viewer

Length: 1050 bp
ATGGCATACATGCACATTTCATGGCTTCTTCTAATTCAATGCCTACTTCTGGTCACCAGAATTGGTGCCAAAGTTCCAGCAATCATTGTGTTCGGAGACTCATCGGTCGATGCTGGGAACAACAACCAGATTCCCACAATTGCCAGGAGCAACTTTCCGCCTTATGGCCGTGACTTTGCCGGTGGCAAGCCTACTGGGAGATTCTCTAATGGCAGAGTTCCCACGGACTTCATCTCCGAAGCTTTTGGACTCAAACCATTCGTACCTGCCTACTTGGATCCTACTTATAACATATCAGATTTTGCAACTGGAGTCACCTTTGCTTCTGCTGGCACTGGCTACGACACAGCAACTTCAGATGTGCTATCTGTGATACCACTATGGAAGCAAGTGCAGTACTACAAGGAATACCAGAGCAAACTGAGACTTCATCTAGGAGGAAACAAAGCAAAGCAAACAATCCATGAAGCTTTGCACATGACAAGCTTAGGAACCAATGATTTCCTTGAAAACTACTACTCATACCCCGGCCGGTCGAATCAATACTCTATCGAACTATACCAAGATTTTCTCATTGGAATTGCAGGAAATTTCATCAAACAACTCTACGGTCTCGGAGCTCGGAAAATATCCTTAGGAGGCCTACCTCCGATGGGGTGCTTGCCATTGGAGAGAACCACCAATATGATGGGTGGAAATGACTGCATTTCAAGTTACAACAATGTGGCCTTGGAGTTCAATGGCAAGTTGAAGACCTTGACTACAAGCCTCAATAAAGAGCTTACTGGGATCAAATTGGTTTTTTCAAACCCATACGATATTTTCATGCAGATTATAAGAAGGCCTTCTTCGTATGGATTCGAGGTGGCTGCGGTGGCTTGCTGTGCTACTGGGATGTTTGAAATGGGCTACGCATGCAATCGAAACAACCCGTTCACCTGTACGGATGCAAGCAAATACATATTCTGGGATTCCTTTCATCCAACTGAGAAAGCAAACCATATAATCTCTGATTATGTGGTGAAAAATGTATTAGCCCAGTTTCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

350

Amino Acids

38.65

Weight (kDa)

8.34

Isoelectric Point (pI)

35.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 28 - 339 1.1e-27 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 288, 836
AasI GACNNNNNNGTC 1 cut(s) 104
Acc36I ACCTGC 1 cut(s) 274
AccB1I GGYRCC 1 cut(s) 65
AciI CCGC 2 cut(s) 158, 872
AclWI GGATC 3 cut(s) 272, 285, 797
AcoI YGGCCR 2 cut(s) 166, 529
AcsI RAATTY 1 cut(s) 589
AcuI CTGAAG 1 cut(s) 339
AfaI GTAC 3 cut(s) 264, 398, 943
AfiI CCNNNNNNNGG 3 cut(s) 49, 164, 653
AgsI TTSAA 7 cut(s) 38, 509, 711, 739, 751, 807, 902
AjnI CCWGG 1 cut(s) 143
AluBI AGCT 5 cut(s) 242, 470, 486, 620, 781
AluI AGCT 5 cut(s) 242, 470, 486, 620, 781
Alw21I GWGCWC 1 cut(s) 622
Alw26I GTCTC 3 cut(s) 90, 418, 617
AlwI GGATC 3 cut(s) 272, 285, 797
AoxI GGCC 5 cut(s) 166, 529, 640, 726, 842
ApeKI GCWGC 1 cut(s) 869
ApoI RAATTY 1 cut(s) 589
Asp700I GAANNNNTTC 1 cut(s) 844
AsuC2I CCSGG 1 cut(s) 528
AsuHPI GGTGA 4 cut(s) 46, 307, 928, 1033
AxyI CCTNAGG 1 cut(s) 634
BamHI GGATCC 1 cut(s) 277
BanI GGYRCC 1 cut(s) 65
BanII GRGCYC 1 cut(s) 622
BbsI GAAGAC 1 cut(s) 758
Bbv12I GWGCWC 1 cut(s) 622
BbvI GCAGC 1 cut(s) 856
BccI CCATC 2 cut(s) 646, 682
BceAI ACGGC 1 cut(s) 153
BcgI CGANNNNNNTGC 2 cut(s) 851, 885
BciT130I CCWGG 1 cut(s) 145
BcnI CCSGG 1 cut(s) 528
BcoDI GTCTC 3 cut(s) 90, 418, 617
BfaI CTAG 1 cut(s) 434
BfuAI ACCTGC 1 cut(s) 274
BisI GCNGC 1 cut(s) 870
BlsI GCNGC 1 cut(s) 871
BmcAI AGTACT 1 cut(s) 398
Bme1390I CCNGG 2 cut(s) 145, 528
BmiI GGNNCC 3 cut(s) 67, 279, 493
BmrFI CCNGG 2 cut(s) 145, 528
BmrI ACTGGG 4 cut(s) 204, 795, 900, 1033
BmsI GCATC 2 cut(s) 100, 937
BmuI ACTGGG 4 cut(s) 204, 795, 900, 1033
BpiI GAAGAC 1 cut(s) 758
BpmI CTGGAG 1 cut(s) 330
Bpu10I CCTNAGC 1 cut(s) 487
BpuMI CCSGG 1 cut(s) 528
BsaI GGTCTC 1 cut(s) 617
BsaJI CCNNGG 3 cut(s) 222, 526, 729
BsaXI ACNNNNNCTCC 2 cut(s) 218, 248
Bsc4I CCNNNNNNNGG 3 cut(s) 49, 164, 653
Bse118I RCCGGY 2 cut(s) 179, 531
Bse1I ACTGG 6 cut(s) 199, 313, 340, 790, 895, 1039
Bse21I CCTNAGG 1 cut(s) 634
BseBI CCWGG 1 cut(s) 145
BseDI CCNNGG 3 cut(s) 222, 526, 729
BseGI GGATG 3 cut(s) 900, 952, 979
BseLI CCNNNNNNNGG 3 cut(s) 49, 164, 653
BseMII CTCAG 2 cut(s) 413, 978
BseNI ACTGG 6 cut(s) 199, 313, 340, 790, 895, 1039
BseX3I CGGCCG 1 cut(s) 529
BseXI GCAGC 1 cut(s) 856
BseYI CCCAGC 1 cut(s) 113
BsgI GTGCAG 1 cut(s) 413
Bsh1285I CGRYCG 3 cut(s) 108, 532, 536
BshFI GGCC 5 cut(s) 168, 531, 642, 728, 844
BshNI GGYRCC 1 cut(s) 65
BsiEI CGRYCG 3 cut(s) 108, 532, 536
BsiHKAI GWGCWC 1 cut(s) 622
BsiSI CCGG 3 cut(s) 180, 528, 532
BslI CCNNNNNNNGG 3 cut(s) 49, 164, 653
BsmAI GTCTC 3 cut(s) 90, 418, 617
BsnI GGCC 5 cut(s) 168, 531, 642, 728, 844
Bso31I GGTCTC 1 cut(s) 617
Bsp1286I GDGCHC 1 cut(s) 622
Bsp143I GATC 2 cut(s) 277, 789
BspACI CCGC 2 cut(s) 158, 872
BspANI GGCC 5 cut(s) 168, 531, 642, 728, 844
BspCNI CTCAG 2 cut(s) 414, 979
BspLI GGNNCC 3 cut(s) 67, 279, 493
BspMI ACCTGC 1 cut(s) 274
BspPI GGATC 3 cut(s) 272, 285, 797
BspT107I GGYRCC 1 cut(s) 65
BspTNI GGTCTC 1 cut(s) 617
BsrFI RCCGGY 2 cut(s) 179, 531
BsrI ACTGG 6 cut(s) 199, 313, 340, 790, 895, 1039
BssAI RCCGGY 2 cut(s) 179, 531
BssECI CCNNGG 3 cut(s) 222, 526, 729
BssMI GATC 2 cut(s) 277, 789
BssT1I CCWWGG 1 cut(s) 729
Bst2UI CCWGG 1 cut(s) 145
Bst4CI ACNGT 1 cut(s) 611
BstC8I GCNNGC 6 cut(s) 188, 331, 662, 880, 916, 952
BstDEI CTNAG 4 cut(s) 422, 487, 634, 987
BstDSI CCRYGG 1 cut(s) 222
BstEII GGTNACC 1 cut(s) 52
BstF5I GGATG 3 cut(s) 900, 952, 979
BstKTI GATC 2 cut(s) 280, 792
BstMAI GTCTC 3 cut(s) 90, 418, 617
BstMBI GATC 2 cut(s) 277, 789
BstMCI CGRYCG 3 cut(s) 108, 532, 536
BstMWI GCNNNNNNNGC 3 cut(s) 875, 884, 915
BstNI CCWGG 1 cut(s) 145
BstNSI RCATGY 2 cut(s) 13, 918
BstPI GGTNACC 1 cut(s) 52
BstSCI CCNGG 2 cut(s) 143, 526
BstV1I GCAGC 1 cut(s) 856
BstV2I GAAGAC 1 cut(s) 758
BstX2I RGATCY 1 cut(s) 277
BstXI CCANNNNNNTGG 1 cut(s) 688
BstYI RGATCY 1 cut(s) 277
BstZI CGGCCG 1 cut(s) 529
Bsu36I CCTNAGG 1 cut(s) 634
BsuRI GGCC 5 cut(s) 168, 531, 642, 728, 844
BtgI CCRYGG 1 cut(s) 222
BtsCI GGATG 3 cut(s) 900, 952, 979
BtsIMutI CAGTG 1 cut(s) 333
BveI ACCTGC 1 cut(s) 274
Cac8I GCNNGC 6 cut(s) 188, 331, 662, 880, 916, 952
Cfr10I RCCGGY 2 cut(s) 179, 531
Csp6I GTAC 3 cut(s) 263, 397, 942
CviAII CATG 6 cut(s) 10, 21, 464, 478, 826, 915
CviQI GTAC 3 cut(s) 263, 397, 942
DdeI CTNAG 4 cut(s) 422, 487, 634, 987
DpnI GATC 2 cut(s) 279, 791
DpnII GATC 2 cut(s) 277, 789
DrdI GACNNNNNNGTC 1 cut(s) 104
DseDI GACNNNNNNGTC 1 cut(s) 104
EaeI YGGCCR 2 cut(s) 166, 529
EagI CGGCCG 1 cut(s) 529
EciI GGCGGA 1 cut(s) 147
Ecl136II GAGCTC 1 cut(s) 620
EclXI CGGCCG 1 cut(s) 529
Eco130I CCWWGG 1 cut(s) 729
Eco147I AGGCCT 2 cut(s) 642, 844
Eco24I GRGCYC 1 cut(s) 622
Eco31I GGTCTC 1 cut(s) 617
Eco52I CGGCCG 1 cut(s) 529
Eco53kI GAGCTC 1 cut(s) 620
Eco57I CTGAAG 1 cut(s) 339
Eco81I CCTNAGG 1 cut(s) 634
Eco91I GGTNACC 1 cut(s) 52
EcoICRI GAGCTC 1 cut(s) 620
EcoO65I GGTNACC 1 cut(s) 52
EcoRII CCWGG 1 cut(s) 143
EcoT14I CCWWGG 1 cut(s) 729
EcoT38I GRGCYC 1 cut(s) 622
ErhI CCWWGG 1 cut(s) 729
FaeI CATG 6 cut(s) 13, 24, 467, 481, 829, 918
FalI AAGNNNNNCTT 4 cut(s) 829, 861, 832, 864
FatI CATG 6 cut(s) 9, 20, 463, 477, 825, 914
Fnu4HI GCNGC 1 cut(s) 870
FokI GGATG 3 cut(s) 907, 959, 966
FriOI GRGCYC 1 cut(s) 622
Fsp4HI GCNGC 1 cut(s) 870
FspBI CTAG 1 cut(s) 434
GluI GCNGC 1 cut(s) 870
GsaI CCCAGC 1 cut(s) 117
GsuI CTGGAG 1 cut(s) 330
HaeIII GGCC 5 cut(s) 168, 531, 642, 728, 844
HapII CCGG 3 cut(s) 180, 528, 532
Hin1II CATG 6 cut(s) 13, 24, 467, 481, 829, 918
HindIII AAGCTT 3 cut(s) 240, 468, 484
HinfI GANTC 8 cut(s) 98, 130, 201, 249, 312, 538, 858, 971
HpaII CCGG 3 cut(s) 180, 528, 532
HphI GGTGA 4 cut(s) 46, 307, 928, 1033
Hpy166II GTNNAC 1 cut(s) 936
Hpy188I TCNGA 8 cut(s) 95, 238, 298, 358, 617, 624, 651, 1012
Hpy8I GTNNAC 1 cut(s) 936
HpyAV CCTTC 2 cut(s) 834, 855
HpyCH4III ACNGT 1 cut(s) 611
HpyCH4V TGCA 9 cut(s) 13, 305, 394, 475, 584, 705, 829, 918, 950
HpyF10VI GCNNNNNNNGC 3 cut(s) 875, 884, 915
HpyF3I CTNAG 4 cut(s) 422, 487, 634, 987
Hsp92II CATG 6 cut(s) 13, 24, 467, 481, 829, 918
Kzo9I GATC 2 cut(s) 277, 789
LmnI GCTCC 2 cut(s) 147, 617
Lsp1109I GCAGC 1 cut(s) 856
LweI GCATC 2 cut(s) 100, 937
MaeI CTAG 1 cut(s) 434
MaeIII GTNAC 4 cut(s) 52, 170, 313, 713
MalI GATC 2 cut(s) 279, 791
MboI GATC 2 cut(s) 277, 789
MboII GAAGA 3 cut(s) 20, 763, 840
MfeI CAATTG 1 cut(s) 138
MflI RGATCY 1 cut(s) 277
MhlI GDGCHC 1 cut(s) 622
MluCI AATT 6 cut(s) 33, 60, 138, 579, 589, 794
MlyI GAGTC 3 cut(s) 92, 243, 321
MmeI TCCRAC 1 cut(s) 1007
MnlI CCTC 5 cut(s) 431, 632, 657, 779, 856
MroXI GAANNNNTTC 1 cut(s) 844
MseI TTAA 1 cut(s) 1048
MslI CAYNNNNRTG 1 cut(s) 683
MspI CCGG 3 cut(s) 180, 528, 532
MspR9I CCNGG 2 cut(s) 145, 528
MunI CAATTG 1 cut(s) 138
MvaI CCWGG 1 cut(s) 145
MwoI GCNNNNNNNGC 3 cut(s) 875, 884, 915
NciI CCSGG 1 cut(s) 528
NdeII GATC 2 cut(s) 277, 789
NlaIII CATG 6 cut(s) 13, 24, 467, 481, 829, 918
NlaIV GGNNCC 3 cut(s) 67, 279, 493
NmuCI GTSAC 3 cut(s) 52, 170, 313
NspI RCATGY 2 cut(s) 13, 918
PaeI GCATGC 1 cut(s) 918
PceI AGGCCT 2 cut(s) 642, 844
PdmI GAANNNNTTC 1 cut(s) 844
PfeI GAWTC 5 cut(s) 130, 201, 538, 858, 971
PkrI GCNGC 1 cut(s) 871
PleI GAGTC 3 cut(s) 92, 243, 320
PpsI GAGTC 3 cut(s) 92, 243, 320
PsiI TTATAA 2 cut(s) 288, 836
Psp124BI GAGCTC 1 cut(s) 622
Psp6I CCWGG 1 cut(s) 143
PspEI GGTNACC 1 cut(s) 52
PspFI CCCAGC 1 cut(s) 113
PspGI CCWGG 1 cut(s) 143
PspN4I GGNNCC 3 cut(s) 67, 279, 493
PsuI RGATCY 1 cut(s) 277
RsaI GTAC 3 cut(s) 264, 398, 943
RsaNI GTAC 3 cut(s) 263, 397, 942
RseI CAYNNNNRTG 1 cut(s) 683
SacI GAGCTC 1 cut(s) 622
SaqAI TTAA 1 cut(s) 1048
SatI GCNGC 1 cut(s) 870
Sau3AI GATC 2 cut(s) 277, 789
ScaI AGTACT 1 cut(s) 398
SchI GAGTC 3 cut(s) 92, 243, 321
ScrFI CCNGG 2 cut(s) 145, 528
SduI GDGCHC 1 cut(s) 622
SfaNI GCATC 2 cut(s) 100, 937
SmiMI CAYNNNNRTG 1 cut(s) 683
SphI GCATGC 1 cut(s) 918
Sse9I AATT 6 cut(s) 33, 60, 138, 579, 589, 794
SseBI AGGCCT 2 cut(s) 642, 844
SsiI CCGC 2 cut(s) 158, 872
SspMI CTAG 1 cut(s) 434
SstI GAGCTC 1 cut(s) 622
StuI AGGCCT 2 cut(s) 642, 844
StyD4I CCNGG 2 cut(s) 143, 526
StyI CCWWGG 1 cut(s) 729
TaaI ACNGT 1 cut(s) 611
TaqI TCGA 5 cut(s) 108, 536, 552, 861, 922
TaqII GACCGA 1 cut(s) 94
TasI AATT 6 cut(s) 33, 60, 138, 579, 589, 794
TatI WGTACW 1 cut(s) 396
TfiI GAWTC 5 cut(s) 130, 201, 538, 858, 971
Tru1I TTAA 1 cut(s) 1048
Tru9I TTAA 1 cut(s) 1048
TscAI CASTG 1 cut(s) 340
TseFI GTSAC 3 cut(s) 52, 170, 313
TseI GCWGC 1 cut(s) 869
Tsp45I GTSAC 3 cut(s) 52, 170, 313
TspDTI ATGAA 7 cut(s) 9, 220, 419, 480, 583, 814, 968
TspGWI ACGGA 2 cut(s) 239, 959
TspRI CASTG 1 cut(s) 340
XapI RAATTY 1 cut(s) 589
XceI RCATGY 2 cut(s) 13, 918
XcmI CCANNNNNNNNNTGG 1 cut(s) 685
XmnI GAANNNNTTC 1 cut(s) 844
XspI CTAG 1 cut(s) 434
ZrmI AGTACT 1 cut(s) 398
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.