Rroxscaffold_2G00091980

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0008949
Physical Location & Seq
Reverse (-)
13538946 .. 13541984
3039 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00091980.1

Sequence Viewer

Length: 828 bp
ATGCTTCAGGCAAAAAACCAACACGCTTGCATTACAGCTATGGCACCCTGGCACATTTCATTTCTGCTCCCAACTCTCCTCCTAGTTCTACTACTAGTCACCAAAGTTGCAGCAAAAGTTCCGGCAGTCATTGTGTTTGGAGACTCCTCCGTCGATGCTGGGAACAACAACCAGATTCCCACAGTTGCCAGGAGCAATTTCGAGCCTTATGGCCGTGATTTCACTGGTGGCAAGCCAACCGGGAGGTTCTCCAACGGCCGAATACCCACGGACTTCATCTCCCAAGCTTTTGGACTCAAACCGACCGTTCCGGCCTACTTGGATCCTTCCTACAGTATATCAGATTTTGCCACTGGTGTCACATTTGCTTCTGCTGGCACCGGCTATGACACTGCAACTTCAGATGTGCTGTCTGTGATATCGCTATGGAAGCAATTGGACTACTACAAGGAATACCAAGCTAAACTGAGAGCTTTCCAGGGAGGAAATGAAGCAAAAGAAACGATCAACGAAGCTTTACACATAATGAGCTTCGGAACTAATGACTTCCTGGAGAACTACTACTCCACATTTCCACCATCAGGCCGATCATCCCAATACACTATCTCCCAATACCAAGACTTTCTCATCGGAATCGCAGCGAATTTCGTGAAGGAACTCTACAAGCTCGGAGCTCGAAAAATTTCCGTGGGAGGACTGCCTCCAATGGGGTGCTTGCCATTAGAGAGAACCAGTAATATCATGGATGGAAATGACTGCATTTCGAATTACAACGATGTGGCTTTGGAGTTCAATAACAAGCTGAATATCTTGACCGTTTTGAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

275

Amino Acids

30.11

Weight (kDa)

6.3

Isoelectric Point (pI)

34.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 43 - 269 1.9e-19 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 149
AccB1I GGYRCC 2 cut(s) 43, 377
AclWI GGATC 2 cut(s) 317, 330
AcoI YGGCCR 2 cut(s) 211, 256
AcsI RAATTY 2 cut(s) 643, 681
AcuI CTGAAG 1 cut(s) 384
AfiI CCNNNNNNNGG 3 cut(s) 581, 707, 822
AgsI TTSAA 1 cut(s) 793
AhlI ACTAGT 1 cut(s) 94
AjnI CCWGG 4 cut(s) 47, 188, 477, 549
AloI GAACNNNNNNTCC 2 cut(s) 548, 580
AluBI AGCT 9 cut(s) 38, 287, 461, 473, 515, 531, 667, 674, 802
AluI AGCT 9 cut(s) 38, 287, 461, 473, 515, 531, 667, 674, 802
Alw21I GWGCWC 1 cut(s) 676
Alw26I GTCTC 1 cut(s) 135
AlwI GGATC 2 cut(s) 317, 330
AoxI GGCC 4 cut(s) 211, 256, 312, 583
ApeKI GCWGC 2 cut(s) 110, 638
ApoI RAATTY 2 cut(s) 643, 681
Asp700I GAANNNNTTC 1 cut(s) 682
AsuC2I CCSGG 1 cut(s) 241
AsuHPI GGTGA 1 cut(s) 91
AsuII TTCGAA 1 cut(s) 764
BamHI GGATCC 1 cut(s) 322
BanI GGYRCC 2 cut(s) 43, 377
BanII GRGCYC 1 cut(s) 676
BarI GAAGNNNNNNTAC 2 cut(s) 644, 676
Bbv12I GWGCWC 1 cut(s) 676
BbvI GCAGC 2 cut(s) 122, 650
BccI CCATC 2 cut(s) 586, 740
BceAI ACGGC 2 cut(s) 198, 271
BciT130I CCWGG 4 cut(s) 49, 190, 479, 551
BcnI CCSGG 1 cut(s) 241
BcoDI GTCTC 1 cut(s) 135
BcuI ACTAGT 1 cut(s) 94
BfaI CTAG 2 cut(s) 83, 95
BfmI CTRYAG 1 cut(s) 331
BisI GCNGC 2 cut(s) 111, 639
BlsI GCNGC 2 cut(s) 112, 640
Bme1390I CCNGG 5 cut(s) 49, 190, 241, 479, 551
BmiI GGNNCC 3 cut(s) 45, 324, 379
BmrFI CCNGG 5 cut(s) 49, 190, 241, 479, 551
BmsI GCATC 1 cut(s) 145
BpmI CTGGAG 1 cut(s) 572
Bpu14I TTCGAA 1 cut(s) 764
BpuMI CCSGG 1 cut(s) 241
BsaJI CCNNGG 4 cut(s) 47, 267, 478, 687
BsaXI ACNNNNNCTCC 6 cut(s) 263, 293, 548, 578, 590, 620
Bsc4I CCNNNNNNNGG 3 cut(s) 581, 707, 822
Bse118I RCCGGY 1 cut(s) 380
Bse1I ACTGG 3 cut(s) 229, 358, 732
BseBI CCWGG 4 cut(s) 49, 190, 479, 551
BseDI CCNNGG 4 cut(s) 47, 267, 478, 687
BseGI GGATG 2 cut(s) 590, 751
BseLI CCNNNNNNNGG 3 cut(s) 581, 707, 822
BseMII CTCAG 1 cut(s) 458
BseNI ACTGG 3 cut(s) 229, 358, 732
BseRI GAGGAG 2 cut(s) 68, 136
BseX3I CGGCCG 1 cut(s) 256
BseXI GCAGC 2 cut(s) 122, 650
BseYI CCCAGC 1 cut(s) 158
Bsh1285I CGRYCG 2 cut(s) 259, 306
BshFI GGCC 4 cut(s) 213, 258, 314, 585
BshNI GGYRCC 2 cut(s) 43, 377
BsiEI CGRYCG 2 cut(s) 259, 306
BsiHKAI GWGCWC 1 cut(s) 676
BsiSI CCGG 4 cut(s) 122, 240, 311, 381
BslI CCNNNNNNNGG 3 cut(s) 581, 707, 822
BsmAI GTCTC 1 cut(s) 135
BsnI GGCC 4 cut(s) 213, 258, 314, 585
Bsp119I TTCGAA 1 cut(s) 764
Bsp1286I GDGCHC 1 cut(s) 676
Bsp143I GATC 3 cut(s) 322, 504, 587
BspANI GGCC 4 cut(s) 213, 258, 314, 585
BspCNI CTCAG 1 cut(s) 459
BspLI GGNNCC 3 cut(s) 45, 324, 379
BspPI GGATC 2 cut(s) 317, 330
BspT104I TTCGAA 1 cut(s) 764
BspT107I GGYRCC 2 cut(s) 43, 377
BsrFI RCCGGY 1 cut(s) 380
BsrI ACTGG 3 cut(s) 229, 358, 732
BssAI RCCGGY 1 cut(s) 380
BssECI CCNNGG 4 cut(s) 47, 267, 478, 687
BssMI GATC 3 cut(s) 322, 504, 587
Bst2UI CCWGG 4 cut(s) 49, 190, 479, 551
Bst4CI ACNGT 4 cut(s) 184, 307, 335, 817
BstBI TTCGAA 1 cut(s) 764
BstC8I GCNNGC 4 cut(s) 28, 233, 376, 716
BstDEI CTNAG 1 cut(s) 467
BstDSI CCRYGG 2 cut(s) 267, 687
BstF5I GGATG 2 cut(s) 590, 751
BstKTI GATC 3 cut(s) 325, 507, 590
BstMAI GTCTC 1 cut(s) 135
BstMBI GATC 3 cut(s) 322, 504, 587
BstMCI CGRYCG 2 cut(s) 259, 306
BstMWI GCNNNNNNNGC 1 cut(s) 430
BstNI CCWGG 4 cut(s) 49, 190, 479, 551
BstSCI CCNGG 5 cut(s) 47, 188, 239, 477, 549
BstSFI CTRYAG 1 cut(s) 331
BstV1I GCAGC 2 cut(s) 122, 650
BstX2I RGATCY 1 cut(s) 322
BstXI CCANNNNNNTGG 1 cut(s) 290
BstYI RGATCY 1 cut(s) 322
BstZI CGGCCG 1 cut(s) 256
BsuRI GGCC 4 cut(s) 213, 258, 314, 585
BtgI CCRYGG 2 cut(s) 267, 687
BtsCI GGATG 2 cut(s) 590, 751
BtsI GCAGTG 1 cut(s) 390
BtsIMutI CAGTG 3 cut(s) 222, 351, 390
Cac8I GCNNGC 4 cut(s) 28, 233, 376, 716
Cfr10I RCCGGY 1 cut(s) 380
CviAII CATG 1 cut(s) 742
DdeI CTNAG 1 cut(s) 467
DpnI GATC 3 cut(s) 324, 506, 589
DpnII GATC 3 cut(s) 322, 504, 587
DrdI GACNNNNNNGTC 1 cut(s) 149
DseDI GACNNNNNNGTC 1 cut(s) 149
EaeI YGGCCR 2 cut(s) 211, 256
EagI CGGCCG 1 cut(s) 256
Ecl136II GAGCTC 1 cut(s) 674
EclXI CGGCCG 1 cut(s) 256
Eco24I GRGCYC 1 cut(s) 676
Eco32I GATATC 1 cut(s) 420
Eco52I CGGCCG 1 cut(s) 256
Eco53kI GAGCTC 1 cut(s) 674
Eco57I CTGAAG 1 cut(s) 384
EcoICRI GAGCTC 1 cut(s) 674
EcoRII CCWGG 4 cut(s) 47, 188, 477, 549
EcoRV GATATC 1 cut(s) 420
EcoT38I GRGCYC 1 cut(s) 676
FaeI CATG 1 cut(s) 745
FaiI YATR 7 cut(s) 41, 210, 338, 387, 427, 524, 743
FatI CATG 1 cut(s) 741
Fnu4HI GCNGC 2 cut(s) 111, 639
FokI GGATG 2 cut(s) 577, 758
FriOI GRGCYC 1 cut(s) 676
Fsp4HI GCNGC 2 cut(s) 111, 639
FspBI CTAG 2 cut(s) 83, 95
GluI GCNGC 2 cut(s) 111, 639
GsaI CCCAGC 1 cut(s) 162
GsuI CTGGAG 1 cut(s) 572
HaeIII GGCC 4 cut(s) 213, 258, 314, 585
HapII CCGG 4 cut(s) 122, 240, 311, 381
Hin1II CATG 1 cut(s) 745
HindIII AAGCTT 2 cut(s) 285, 513
HinfI GANTC 4 cut(s) 143, 175, 294, 633
HpaII CCGG 4 cut(s) 122, 240, 311, 381
HphI GGTGA 1 cut(s) 91
Hpy188I TCNGA 5 cut(s) 343, 403, 536, 632, 671
Hpy188III TCNNGA 2 cut(s) 649, 811
Hpy99I CGWCG 1 cut(s) 155
HpyAV CCTTC 2 cut(s) 336, 646
HpyCH4III ACNGT 4 cut(s) 184, 307, 335, 817
HpyCH4V TGCA 4 cut(s) 30, 110, 395, 759
HpyF10VI GCNNNNNNNGC 1 cut(s) 430
HpyF3I CTNAG 1 cut(s) 467
Hsp92II CATG 1 cut(s) 745
Kzo9I GATC 3 cut(s) 322, 504, 587
LmnI GCTCC 3 cut(s) 72, 192, 671
Lsp1109I GCAGC 2 cut(s) 122, 650
LweI GCATC 1 cut(s) 145
MaeI CTAG 2 cut(s) 83, 95
MaeIII GTNAC 2 cut(s) 97, 358
MalI GATC 3 cut(s) 324, 506, 589
MboI GATC 3 cut(s) 322, 504, 587
MfeI CAATTG 1 cut(s) 434
MflI RGATCY 1 cut(s) 322
MhlI GDGCHC 1 cut(s) 676
MluCI AATT 5 cut(s) 196, 434, 643, 681, 766
MlyI GAGTC 2 cut(s) 137, 288
MmeI TCCRAC 1 cut(s) 276
MnlI CCTC 7 cut(s) 89, 157, 237, 476, 686, 711, 816
MroXI GAANNNNTTC 1 cut(s) 682
MspI CCGG 4 cut(s) 122, 240, 311, 381
MspR9I CCNGG 5 cut(s) 49, 190, 241, 479, 551
MunI CAATTG 1 cut(s) 434
MvaI CCWGG 4 cut(s) 49, 190, 479, 551
MwoI GCNNNNNNNGC 1 cut(s) 430
NciI CCSGG 1 cut(s) 241
NdeII GATC 3 cut(s) 322, 504, 587
NlaIII CATG 1 cut(s) 745
NlaIV GGNNCC 3 cut(s) 45, 324, 379
NmuCI GTSAC 2 cut(s) 97, 358
NspV TTCGAA 1 cut(s) 764
PdmI GAANNNNTTC 1 cut(s) 682
PfeI GAWTC 2 cut(s) 175, 633
PfoI TCCNGGA 1 cut(s) 549
PkrI GCNGC 2 cut(s) 112, 640
PleI GAGTC 2 cut(s) 137, 288
PpsI GAGTC 2 cut(s) 137, 288
Psp124BI GAGCTC 1 cut(s) 676
Psp6I CCWGG 4 cut(s) 47, 188, 477, 549
PspFI CCCAGC 1 cut(s) 158
PspGI CCWGG 4 cut(s) 47, 188, 477, 549
PspN4I GGNNCC 3 cut(s) 45, 324, 379
PsuI RGATCY 1 cut(s) 322
SacI GAGCTC 1 cut(s) 676
SatI GCNGC 2 cut(s) 111, 639
Sau3AI GATC 3 cut(s) 322, 504, 587
SchI GAGTC 2 cut(s) 137, 288
ScrFI CCNGG 5 cut(s) 49, 190, 241, 479, 551
SduI GDGCHC 1 cut(s) 676
SfaNI GCATC 1 cut(s) 145
SfcI CTRYAG 1 cut(s) 331
SfuI TTCGAA 1 cut(s) 764
SpeI ACTAGT 1 cut(s) 94
Sse9I AATT 5 cut(s) 196, 434, 643, 681, 766
SspMI CTAG 2 cut(s) 83, 95
SstI GAGCTC 1 cut(s) 676
StyD4I CCNGG 5 cut(s) 47, 188, 239, 477, 549
TaaI ACNGT 4 cut(s) 184, 307, 335, 817
TaqI TCGA 4 cut(s) 153, 201, 676, 764
TasI AATT 5 cut(s) 196, 434, 643, 681, 766
TfiI GAWTC 2 cut(s) 175, 633
TscAI CASTG 3 cut(s) 229, 358, 397
TseFI GTSAC 2 cut(s) 97, 358
TseI GCWGC 2 cut(s) 110, 638
Tsp45I GTSAC 2 cut(s) 97, 358
TspDTI ATGAA 3 cut(s) 48, 265, 504
TspGWI ACGGA 3 cut(s) 139, 284, 676
TspRI CASTG 3 cut(s) 229, 358, 397
XapI RAATTY 2 cut(s) 643, 681
XcmI CCANNNNNNNNNTGG 1 cut(s) 739
XmnI GAANNNNTTC 1 cut(s) 682
XspI CTAG 2 cut(s) 83, 95
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.