MD09G1116400.v1.1

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0008949
Physical Location & Seq
Reverse (-)
8939579 .. 8942902
3324 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1116400.v1.1.491

Sequence Viewer

Length: 1053 bp
ATGGCATACCTGCACATTTCATGGCTTCTTCTAGCTCAATGCCTCCTACTACTAGTCACAAGAATTGTTGCCAAAGTTCCGGCAGTCGTGGTGTTCGGGGACTCGTCGGTGGATGCTGGGAACAACAACCAGATTCAAACAGTTTTGAAGAGCAATTTCCAGCCCTATGGTCGTGACTTTGAAGGTGGCAAGCCAACTGGGAGGTTCTCCAATGGCAGAGTGCCTACGGACTTCATCTCCAACGCTTTTGGACTTAGATCATCCGTACCTGCCTACTTGGATCCTGCTTATAACATATCAGATTTTGCAGTCGGAGTTACTTTTGCTTCTGCTGGCACTGGCTATGACACTGCAACTTCAGATGTGCTATCAGTGATACCACTATGGAAGCAGTTGCAGTACTACAAAGAATTTCAGAACAAGTTGAGACTTTATATGGGAGGAAACAAAGCAGACCAAACCATCCATGAAGCTTTGCACATGATGAGCTTAGGAACCAATGACTTCCTTGAAAACTACTACACATACCCCGGCCGGTCGAATCAATACTCTATCGAACAATACCAAGATTTTCTCATTGGAATTGCAGGAAATTTCATCAAACAACTCTACGATCTCGGAGCTCGGAAAATATCCTTAGGAGGCCTACCTCCAATGGGGTGCTTGCCACTGGAGAGAACCACAAATTTCATGGGTGGAAATGACTGCATTTCAGATTACAACAATGTGGCTTTAGAGTTCAATGGCAAGCTGAAGACCTTGACTACAAATCTGAATAAACAGCTTCCTGGGATCAAATTGGTTTTTTCAAACCCATATTACATTTTCCTGCATATGATAAGAAGGCCTTCTTTTTATGGATTCGAGGTGTCATCGGCGGCGTGTTGTGCCAGTGGAATGTTTGAAATGGGCTACGCATGCAATCGAAACAACTTGCTCACTTGCACAGACGCAAGCAAATACATTTTCTGGGATGCCTTTCATCCTACTGAAAAAGCAAACCGTATAATCTCTGATTATGTGGTGAAAAAAGTACTTGCCCAGTTCCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

351

Amino Acids

39.09

Weight (kDa)

8.34

Isoelectric Point (pI)

34.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 29 - 340 4.2e-29 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 291
Acc36I ACCTGC 2 cut(s) 18, 277
AciI CCGC 1 cut(s) 878
AclWI GGATC 3 cut(s) 275, 288, 800
AcoI YGGCCR 1 cut(s) 532
AcsI RAATTY 3 cut(s) 410, 592, 685
AcuI CTGAAG 2 cut(s) 342, 773
AfaI GTAC 3 cut(s) 267, 401, 1035
AfiI CCNNNNNNNGG 1 cut(s) 656
AgsI TTSAA 7 cut(s) 137, 148, 182, 512, 742, 810, 905
AhlI ACTAGT 1 cut(s) 52
AjnI CCWGG 1 cut(s) 787
AluBI AGCT 6 cut(s) 35, 473, 489, 623, 751, 784
AluI AGCT 6 cut(s) 35, 473, 489, 623, 751, 784
Alw21I GWGCWC 1 cut(s) 625
Alw26I GTCTC 1 cut(s) 421
AlwI GGATC 3 cut(s) 275, 288, 800
AoxI GGCC 3 cut(s) 532, 643, 845
ApoI RAATTY 3 cut(s) 410, 592, 685
Asp700I GAANNNNTTC 1 cut(s) 847
AsuC2I CCSGG 1 cut(s) 531
AsuHPI GGTGA 1 cut(s) 1036
AxyI CCTNAGG 1 cut(s) 637
BamHI GGATCC 1 cut(s) 280
BanII GRGCYC 1 cut(s) 625
BbsI GAAGAC 1 cut(s) 761
Bbv12I GWGCWC 1 cut(s) 625
BccI CCATC 1 cut(s) 470
BciT130I CCWGG 1 cut(s) 789
BcnI CCSGG 1 cut(s) 531
BcoDI GTCTC 1 cut(s) 421
BcuI ACTAGT 1 cut(s) 52
BfaI CTAG 2 cut(s) 32, 53
BfuAI ACCTGC 2 cut(s) 18, 277
BisI GCNGC 1 cut(s) 879
BlsI GCNGC 1 cut(s) 880
BmcAI AGTACT 2 cut(s) 401, 1035
Bme1390I CCNGG 2 cut(s) 531, 789
BmiI GGNNCC 2 cut(s) 282, 496
BmrFI CCNGG 2 cut(s) 531, 789
BmrI ACTGGG 2 cut(s) 207, 1036
BmsI GCATC 2 cut(s) 103, 964
BmuI ACTGGG 2 cut(s) 207, 1036
BpiI GAAGAC 1 cut(s) 761
BpmI CTGGAG 1 cut(s) 692
Bpu10I CCTNAGC 1 cut(s) 490
BpuMI CCSGG 1 cut(s) 531
BsaJI CCNNGG 2 cut(s) 529, 788
BsaXI ACNNNNNCTCC 2 cut(s) 221, 251
Bsc4I CCNNNNNNNGG 1 cut(s) 656
Bse118I RCCGGY 1 cut(s) 534
Bse1I ACTGG 5 cut(s) 202, 343, 675, 891, 1042
Bse21I CCTNAGG 1 cut(s) 637
BseBI CCWGG 1 cut(s) 789
BseDI CCNNGG 2 cut(s) 529, 788
BseGI GGATG 5 cut(s) 118, 260, 462, 979, 982
BseLI CCNNNNNNNGG 1 cut(s) 656
BseNI ACTGG 5 cut(s) 202, 343, 675, 891, 1042
BseX3I CGGCCG 1 cut(s) 532
BseYI CCCAGC 1 cut(s) 116
Bsh1285I CGRYCG 2 cut(s) 535, 539
BshFI GGCC 3 cut(s) 534, 645, 847
BsiEI CGRYCG 2 cut(s) 535, 539
BsiHKAI GWGCWC 1 cut(s) 625
BsiSI CCGG 3 cut(s) 80, 531, 535
BslFI GGGAC 1 cut(s) 113
BslI CCNNNNNNNGG 1 cut(s) 656
BsmAI GTCTC 1 cut(s) 421
BsmFI GGGAC 1 cut(s) 113
BsnI GGCC 3 cut(s) 534, 645, 847
Bsp1286I GDGCHC 1 cut(s) 625
Bsp143I GATC 4 cut(s) 257, 280, 613, 792
BspACI CCGC 1 cut(s) 878
BspANI GGCC 3 cut(s) 534, 645, 847
BspLI GGNNCC 2 cut(s) 282, 496
BspMI ACCTGC 2 cut(s) 18, 277
BspPI GGATC 3 cut(s) 275, 288, 800
BspQI GCTCTTC 1 cut(s) 143
BsrFI RCCGGY 1 cut(s) 534
BsrI ACTGG 5 cut(s) 202, 343, 675, 891, 1042
BssAI RCCGGY 1 cut(s) 534
BssECI CCNNGG 2 cut(s) 529, 788
BssMI GATC 4 cut(s) 257, 280, 613, 792
Bst2UI CCWGG 1 cut(s) 789
Bst4CI ACNGT 2 cut(s) 142, 1004
Bst6I CTCTTC 1 cut(s) 143
BstC8I GCNNGC 6 cut(s) 191, 334, 665, 749, 919, 955
BstDEI CTNAG 3 cut(s) 254, 490, 637
BstF5I GGATG 5 cut(s) 118, 260, 462, 979, 982
BstKTI GATC 4 cut(s) 260, 283, 616, 795
BstMAI GTCTC 1 cut(s) 421
BstMBI GATC 4 cut(s) 257, 280, 613, 792
BstMCI CGRYCG 2 cut(s) 535, 539
BstMWI GCNNNNNNNGC 2 cut(s) 887, 918
BstNI CCWGG 1 cut(s) 789
BstNSI RCATGY 1 cut(s) 921
BstSCI CCNGG 2 cut(s) 529, 787
BstV2I GAAGAC 1 cut(s) 761
BstX2I RGATCY 1 cut(s) 280
BstXI CCANNNNNNTGG 1 cut(s) 167
BstYI RGATCY 1 cut(s) 280
BstZI CGGCCG 1 cut(s) 532
Bsu36I CCTNAGG 1 cut(s) 637
BsuRI GGCC 3 cut(s) 534, 645, 847
BtsCI GGATG 5 cut(s) 118, 260, 462, 979, 982
BtsI GCAGTG 1 cut(s) 348
BtsIMutI CAGTG 5 cut(s) 336, 348, 378, 668, 898
BveI ACCTGC 2 cut(s) 18, 277
Cac8I GCNNGC 6 cut(s) 191, 334, 665, 749, 919, 955
Cfr10I RCCGGY 1 cut(s) 534
CseI GACGC 1 cut(s) 959
Csp6I GTAC 3 cut(s) 266, 400, 1034
CviAII CATG 5 cut(s) 21, 467, 481, 691, 918
CviQI GTAC 3 cut(s) 266, 400, 1034
DdeI CTNAG 3 cut(s) 254, 490, 637
DpnI GATC 4 cut(s) 259, 282, 615, 794
DpnII GATC 4 cut(s) 257, 280, 613, 792
EaeI YGGCCR 1 cut(s) 532
EagI CGGCCG 1 cut(s) 532
Eam1104I CTCTTC 1 cut(s) 143
EarI CTCTTC 1 cut(s) 143
Ecl136II GAGCTC 1 cut(s) 623
EclXI CGGCCG 1 cut(s) 532
Eco147I AGGCCT 2 cut(s) 645, 847
Eco24I GRGCYC 1 cut(s) 625
Eco52I CGGCCG 1 cut(s) 532
Eco53kI GAGCTC 1 cut(s) 623
Eco57I CTGAAG 2 cut(s) 342, 773
Eco81I CCTNAGG 1 cut(s) 637
EcoICRI GAGCTC 1 cut(s) 623
EcoRII CCWGG 1 cut(s) 787
EcoT38I GRGCYC 1 cut(s) 625
FaeI CATG 5 cut(s) 24, 470, 484, 694, 921
FalI AAGNNNNNCTT 4 cut(s) 832, 864, 835, 867
FaqI GGGAC 1 cut(s) 113
FatI CATG 5 cut(s) 20, 466, 480, 690, 917
FauNDI CATATG 1 cut(s) 834
Fnu4HI GCNGC 1 cut(s) 879
FokI GGATG 5 cut(s) 125, 247, 449, 969, 986
FriOI GRGCYC 1 cut(s) 625
Fsp4HI GCNGC 1 cut(s) 879
FspBI CTAG 2 cut(s) 32, 53
GluI GCNGC 1 cut(s) 879
GsaI CCCAGC 1 cut(s) 120
GsuI CTGGAG 1 cut(s) 692
HaeIII GGCC 3 cut(s) 534, 645, 847
HapII CCGG 3 cut(s) 80, 531, 535
HgaI GACGC 1 cut(s) 959
Hin1II CATG 5 cut(s) 24, 470, 484, 694, 921
HindIII AAGCTT 1 cut(s) 471
HinfI GANTC 4 cut(s) 101, 133, 541, 861
HpaII CCGG 3 cut(s) 80, 531, 535
HphI GGTGA 1 cut(s) 1036
Hpy188I TCNGA 9 cut(s) 301, 314, 361, 417, 620, 627, 715, 774, 1015
Hpy188III TCNNGA 1 cut(s) 173
Hpy99I CGWCG 1 cut(s) 109
HpyAV CCTTC 3 cut(s) 176, 837, 858
HpyCH4III ACNGT 2 cut(s) 142, 1004
HpyF10VI GCNNNNNNNGC 2 cut(s) 887, 918
HpyF3I CTNAG 3 cut(s) 254, 490, 637
Hsp92II CATG 5 cut(s) 24, 470, 484, 694, 921
Kzo9I GATC 4 cut(s) 257, 280, 613, 792
LguI GCTCTTC 1 cut(s) 143
LmnI GCTCC 1 cut(s) 620
LweI GCATC 2 cut(s) 103, 964
MaeI CTAG 2 cut(s) 32, 53
MaeIII GTNAC 3 cut(s) 55, 173, 316
MalI GATC 4 cut(s) 259, 282, 615, 794
MboI GATC 4 cut(s) 257, 280, 613, 792
MboII GAAGA 3 cut(s) 20, 160, 766
MflI RGATCY 1 cut(s) 280
MhlI GDGCHC 1 cut(s) 625
MluCI AATT 7 cut(s) 63, 154, 410, 582, 592, 685, 797
MlyI GAGTC 1 cut(s) 95
MmeI TCCRAC 2 cut(s) 264, 292
MnlI CCTC 6 cut(s) 53, 195, 434, 635, 660, 859
MroXI GAANNNNTTC 1 cut(s) 847
MspI CCGG 3 cut(s) 80, 531, 535
MspR9I CCNGG 2 cut(s) 531, 789
MvaI CCWGG 1 cut(s) 789
MwoI GCNNNNNNNGC 2 cut(s) 887, 918
NciI CCSGG 1 cut(s) 531
NdeI CATATG 1 cut(s) 834
NdeII GATC 4 cut(s) 257, 280, 613, 792
NlaIII CATG 5 cut(s) 24, 470, 484, 694, 921
NlaIV GGNNCC 2 cut(s) 282, 496
NmuCI GTSAC 2 cut(s) 55, 173
NspI RCATGY 1 cut(s) 921
PaeI GCATGC 1 cut(s) 921
PceI AGGCCT 2 cut(s) 645, 847
PciSI GCTCTTC 1 cut(s) 143
PdmI GAANNNNTTC 1 cut(s) 847
PfeI GAWTC 3 cut(s) 133, 541, 861
PkrI GCNGC 1 cut(s) 880
PleI GAGTC 1 cut(s) 95
PpsI GAGTC 1 cut(s) 95
PsiI TTATAA 1 cut(s) 291
Psp124BI GAGCTC 1 cut(s) 625
Psp6I CCWGG 1 cut(s) 787
PspFI CCCAGC 1 cut(s) 116
PspGI CCWGG 1 cut(s) 787
PspN4I GGNNCC 2 cut(s) 282, 496
PsuI RGATCY 1 cut(s) 280
RsaI GTAC 3 cut(s) 267, 401, 1035
RsaNI GTAC 3 cut(s) 266, 400, 1034
SacI GAGCTC 1 cut(s) 625
SapI GCTCTTC 1 cut(s) 143
SatI GCNGC 1 cut(s) 879
Sau3AI GATC 4 cut(s) 257, 280, 613, 792
ScaI AGTACT 2 cut(s) 401, 1035
SchI GAGTC 1 cut(s) 95
ScrFI CCNGG 2 cut(s) 531, 789
SduI GDGCHC 1 cut(s) 625
SfaNI GCATC 2 cut(s) 103, 964
SpeI ACTAGT 1 cut(s) 52
SphI GCATGC 1 cut(s) 921
Sse9I AATT 7 cut(s) 63, 154, 410, 582, 592, 685, 797
SseBI AGGCCT 2 cut(s) 645, 847
SsiI CCGC 1 cut(s) 878
SspMI CTAG 2 cut(s) 32, 53
SstI GAGCTC 1 cut(s) 625
StuI AGGCCT 2 cut(s) 645, 847
StyD4I CCNGG 2 cut(s) 529, 787
TaaI ACNGT 2 cut(s) 142, 1004
TaqI TCGA 4 cut(s) 539, 555, 864, 925
TasI AATT 7 cut(s) 63, 154, 410, 582, 592, 685, 797
TatI WGTACW 2 cut(s) 399, 1033
TauI GCSGC 1 cut(s) 881
TfiI GAWTC 3 cut(s) 133, 541, 861
TscAI CASTG 5 cut(s) 343, 355, 378, 675, 898
TseFI GTSAC 2 cut(s) 55, 173
Tsp45I GTSAC 2 cut(s) 55, 173
TspDTI ATGAA 6 cut(s) 9, 223, 483, 586, 679, 971
TspGWI ACGGA 2 cut(s) 242, 253
TspRI CASTG 5 cut(s) 343, 355, 378, 675, 898
XapI RAATTY 3 cut(s) 410, 592, 685
XceI RCATGY 1 cut(s) 921
XcmI CCANNNNNNNNNTGG 1 cut(s) 688
XmnI GAANNNNTTC 1 cut(s) 847
XspI CTAG 2 cut(s) 32, 53
ZrmI AGTACT 2 cut(s) 401, 1035
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.