RLG00000021016

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0008949
Physical Location & Seq
Forward (+)
71102945 .. 71105388
2444 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000021016

Sequence Viewer

Length: 1065 bp
ATGGCACCCTGGCACATTTCATTTCTGCTCCCAACTCTCCTCCTACTTCTACTACTAGTCACCAAAGTTGCAGCAAAAGTTCCGGCAATCATTGTGTTCGGAGACTCCTCCGTCGATGCAGGGAACAACAACCAGATTCCCACCGTTGCTAGGAGCAATTTCGAGCCTTATGGCCGTGATTTCACTGGTGGCAAGCCAACCGGGAGGTTCTCCAACGGCCGAATAGCCACGGACTTCATCTCGAAAGCTTTTGGACTCAAACTGACCGTTCCGGCCTACTTGGATCCTTCCTACAGTATATCAGATTTTGCCACTGGTGTCACATTTGCTTCTGCTGGCACCGGCTATGATACTGCAACTTCAGATGTGCTGTCTGTGATACCGCTATGGAAGCAATTGGACTACTACAAGGAATACCAAGCTAAACTGAGAGCTTACCAGGGAGGAAATGAAGCAAAAGAAACAATCAACGAAGCTTTACACGTGATAAGCTTAGGAACTAATGACTTCCTGGAGAACTACTACTCCACATTTCCACCATCAGGCCGATCATCCCAATACACTATCTCCCAATACCAAGACTTTCTCATTGGAATTGCAGCGAATTTCGTGAAGGAACTCTACAAGCTCGGAGCTCGGAAAATTTCCGTGGGAGGACTGCCTCCAATGGGGTGCTTGCCATTAGAGAGAACCAGTAATGTCATGGATGGAAATGACTGCATTTCGAATTACAACGATGTGGCTTTGGAGTTCAATAACAAGCTAAATATCTTGACCGTAAGCCTTAATAAAGAGCTTCCTGGAAGCAAATTGGTGTTCTCAAATCCTTATTTTGTTTTCCTGCATATGATAAGAAGGCCTTCTTTTTATGGTTTTGATGTGACATCAGTGGCTTGCTGTGCCACAGGAATGTTTGAGATGGGCTATGCATGCAACCGAAACAACATGCTCACATGCACTGATGCAAGCAAATACATCTTCTGGGACTCCTTCCATCCCACAGAGAAAGCAAACCAGATCATCTCTGATTACTTGGTCAAACGTGCCCTGACCCAGTTTCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

355

Amino Acids

39.19

Weight (kDa)

6.89

Isoelectric Point (pI)

31.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 30 - 344 1.9e-30 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 110
AccB1I GGYRCC 2 cut(s) 4, 338
AciI CCGC 1 cut(s) 383
AclWI GGATC 2 cut(s) 278, 291
AcoI YGGCCR 2 cut(s) 172, 217
AcsI RAATTY 2 cut(s) 604, 642
AcuI CTGAAG 1 cut(s) 345
AcvI CACGTG 1 cut(s) 484
AfiI CCNNNNNNNGG 3 cut(s) 150, 542, 668
AflIII ACRYGT 1 cut(s) 481
AgsI TTSAA 1 cut(s) 754
AhlI ACTAGT 1 cut(s) 55
AjnI CCWGG 4 cut(s) 8, 438, 510, 799
AloI GAACNNNNNNTCC 2 cut(s) 509, 541
AluBI AGCT 9 cut(s) 248, 422, 434, 476, 492, 628, 635, 763, 796
AluI AGCT 9 cut(s) 248, 422, 434, 476, 492, 628, 635, 763, 796
Alw21I GWGCWC 1 cut(s) 637
Alw26I GTCTC 1 cut(s) 96
AlwI GGATC 2 cut(s) 278, 291
AoxI GGCC 5 cut(s) 172, 217, 273, 544, 857
ApeKI GCWGC 2 cut(s) 71, 599
ApoI RAATTY 2 cut(s) 604, 642
Asp700I GAANNNNTTC 1 cut(s) 859
AsuC2I CCSGG 1 cut(s) 202
AsuHPI GGTGA 1 cut(s) 52
AsuII TTCGAA 1 cut(s) 725
BaeGI GKGCMC 1 cut(s) 1048
BamHI GGATCC 1 cut(s) 283
BanI GGYRCC 2 cut(s) 4, 338
BanII GRGCYC 1 cut(s) 637
BarI GAAGNNNNNNTAC 2 cut(s) 605, 637
BbrPI CACGTG 1 cut(s) 484
Bbv12I GWGCWC 1 cut(s) 637
BbvI GCAGC 2 cut(s) 83, 611
BccI CCATC 4 cut(s) 547, 701, 913, 1002
BceAI ACGGC 2 cut(s) 159, 232
BciT130I CCWGG 4 cut(s) 10, 440, 512, 801
BcnI CCSGG 1 cut(s) 202
BcoDI GTCTC 1 cut(s) 96
BcuI ACTAGT 1 cut(s) 55
BfaI CTAG 2 cut(s) 56, 150
BfmI CTRYAG 1 cut(s) 292
BisI GCNGC 2 cut(s) 72, 600
BlsI GCNGC 2 cut(s) 73, 601
Bme1390I CCNGG 5 cut(s) 10, 202, 440, 512, 801
BmiI GGNNCC 3 cut(s) 6, 285, 340
BmrFI CCNGG 5 cut(s) 10, 202, 440, 512, 801
BmrI ACTGGG 1 cut(s) 1048
BmsI GCATC 2 cut(s) 106, 952
BmuI ACTGGG 1 cut(s) 1048
BpmI CTGGAG 1 cut(s) 533
Bpu10I CCTNAGC 1 cut(s) 493
Bpu14I TTCGAA 1 cut(s) 725
BpuMI CCSGG 1 cut(s) 202
BsaAI YACGTR 1 cut(s) 484
BsaJI CCNNGG 4 cut(s) 8, 228, 439, 648
BsaXI ACNNNNNCTCC 4 cut(s) 509, 539, 551, 581
Bsc4I CCNNNNNNNGG 3 cut(s) 150, 542, 668
Bse118I RCCGGY 1 cut(s) 341
Bse1I ACTGG 4 cut(s) 190, 319, 693, 1054
BseBI CCWGG 4 cut(s) 10, 440, 512, 801
BseDI CCNNGG 4 cut(s) 8, 228, 439, 648
BseGI GGATG 3 cut(s) 551, 712, 994
BseLI CCNNNNNNNGG 3 cut(s) 150, 542, 668
BseMII CTCAG 1 cut(s) 419
BseNI ACTGG 4 cut(s) 190, 319, 693, 1054
BseRI GAGGAG 2 cut(s) 29, 97
BseSI GKGCMC 1 cut(s) 1048
BseX3I CGGCCG 1 cut(s) 217
BseXI GCAGC 2 cut(s) 83, 611
Bsh1285I CGRYCG 1 cut(s) 220
BshFI GGCC 5 cut(s) 174, 219, 275, 546, 859
BshNI GGYRCC 2 cut(s) 4, 338
BsiEI CGRYCG 1 cut(s) 220
BsiHKAI GWGCWC 1 cut(s) 637
BsiSI CCGG 4 cut(s) 83, 201, 272, 342
BslFI GGGAC 1 cut(s) 998
BslI CCNNNNNNNGG 3 cut(s) 150, 542, 668
BsmAI GTCTC 1 cut(s) 96
BsmFI GGGAC 1 cut(s) 998
BsnI GGCC 5 cut(s) 174, 219, 275, 546, 859
Bsp119I TTCGAA 1 cut(s) 725
Bsp1286I GDGCHC 2 cut(s) 637, 1048
Bsp143I GATC 3 cut(s) 283, 548, 1017
BspACI CCGC 1 cut(s) 383
BspANI GGCC 5 cut(s) 174, 219, 275, 546, 859
BspCNI CTCAG 1 cut(s) 420
BspLI GGNNCC 3 cut(s) 6, 285, 340
BspPI GGATC 2 cut(s) 278, 291
BspT104I TTCGAA 1 cut(s) 725
BspT107I GGYRCC 2 cut(s) 4, 338
BsrFI RCCGGY 1 cut(s) 341
BsrI ACTGG 4 cut(s) 190, 319, 693, 1054
BssAI RCCGGY 1 cut(s) 341
BssECI CCNNGG 4 cut(s) 8, 228, 439, 648
BssMI GATC 3 cut(s) 283, 548, 1017
Bst2UI CCWGG 4 cut(s) 10, 440, 512, 801
Bst4CI ACNGT 4 cut(s) 145, 268, 296, 778
BstBAI YACGTR 1 cut(s) 484
BstBI TTCGAA 1 cut(s) 725
BstC8I GCNNGC 6 cut(s) 194, 337, 677, 895, 931, 967
BstDEI CTNAG 2 cut(s) 428, 493
BstDSI CCRYGG 2 cut(s) 228, 648
BstF5I GGATG 3 cut(s) 551, 712, 994
BstKTI GATC 3 cut(s) 286, 551, 1020
BstMAI GTCTC 1 cut(s) 96
BstMBI GATC 3 cut(s) 283, 548, 1017
BstMCI CGRYCG 1 cut(s) 220
BstMWI GCNNNNNNNGC 3 cut(s) 391, 899, 930
BstNI CCWGG 4 cut(s) 10, 440, 512, 801
BstNSI RCATGY 3 cut(s) 933, 949, 957
BstSCI CCNGG 5 cut(s) 8, 200, 438, 510, 799
BstSFI CTRYAG 1 cut(s) 292
BstSLI GKGCMC 1 cut(s) 1048
BstV1I GCAGC 2 cut(s) 83, 611
BstX2I RGATCY 1 cut(s) 283
BstYI RGATCY 1 cut(s) 283
BstZI CGGCCG 1 cut(s) 217
BsuRI GGCC 5 cut(s) 174, 219, 275, 546, 859
BtgI CCRYGG 2 cut(s) 228, 648
BtsCI GGATG 3 cut(s) 551, 712, 994
BtsIMutI CAGTG 4 cut(s) 183, 312, 894, 957
Cac8I GCNNGC 6 cut(s) 194, 337, 677, 895, 931, 967
Cfr10I RCCGGY 1 cut(s) 341
CviAII CATG 4 cut(s) 703, 930, 946, 954
DdeI CTNAG 2 cut(s) 428, 493
DpnI GATC 3 cut(s) 285, 550, 1019
DpnII GATC 3 cut(s) 283, 548, 1017
DrdI GACNNNNNNGTC 1 cut(s) 110
DseDI GACNNNNNNGTC 1 cut(s) 110
EaeI YGGCCR 2 cut(s) 172, 217
EagI CGGCCG 1 cut(s) 217
Ecl136II GAGCTC 1 cut(s) 635
EclXI CGGCCG 1 cut(s) 217
Eco147I AGGCCT 1 cut(s) 859
Eco24I GRGCYC 1 cut(s) 637
Eco52I CGGCCG 1 cut(s) 217
Eco53kI GAGCTC 1 cut(s) 635
Eco57I CTGAAG 1 cut(s) 345
Eco72I CACGTG 1 cut(s) 484
EcoICRI GAGCTC 1 cut(s) 635
EcoRII CCWGG 4 cut(s) 8, 438, 510, 799
EcoT22I ATGCAT 1 cut(s) 931
EcoT38I GRGCYC 1 cut(s) 637
FaeI CATG 4 cut(s) 706, 933, 949, 957
FalI AAGNNNNNCTT 4 cut(s) 844, 876, 847, 879
FaqI GGGAC 1 cut(s) 998
FatI CATG 4 cut(s) 702, 929, 945, 953
FauNDI CATATG 1 cut(s) 846
Fnu4HI GCNGC 2 cut(s) 72, 600
FokI GGATG 3 cut(s) 538, 719, 981
FriOI GRGCYC 1 cut(s) 637
Fsp4HI GCNGC 2 cut(s) 72, 600
FspBI CTAG 2 cut(s) 56, 150
GluI GCNGC 2 cut(s) 72, 600
GsuI CTGGAG 1 cut(s) 533
HaeIII GGCC 5 cut(s) 174, 219, 275, 546, 859
HapII CCGG 4 cut(s) 83, 201, 272, 342
Hin1II CATG 4 cut(s) 706, 933, 949, 957
HindIII AAGCTT 3 cut(s) 246, 474, 490
HinfI GANTC 4 cut(s) 104, 136, 255, 986
HpaII CCGG 4 cut(s) 83, 201, 272, 342
HphI GGTGA 1 cut(s) 52
Hpy188I TCNGA 6 cut(s) 101, 304, 364, 632, 639, 1027
Hpy188III TCNNGA 3 cut(s) 241, 610, 772
Hpy99I CGWCG 1 cut(s) 116
HpyAV CCTTC 5 cut(s) 297, 607, 849, 870, 1000
HpyCH4III ACNGT 4 cut(s) 145, 268, 296, 778
HpyCH4IV ACGT 2 cut(s) 483, 1042
HpyF10VI GCNNNNNNNGC 3 cut(s) 391, 899, 930
HpyF3I CTNAG 2 cut(s) 428, 493
HpySE526I ACGT 2 cut(s) 483, 1042
Hsp92II CATG 4 cut(s) 706, 933, 949, 957
Kzo9I GATC 3 cut(s) 283, 548, 1017
LmnI GCTCC 3 cut(s) 33, 153, 632
Lsp1109I GCAGC 2 cut(s) 83, 611
LweI GCATC 2 cut(s) 106, 952
MaeI CTAG 2 cut(s) 56, 150
MaeII ACGT 2 cut(s) 483, 1042
MaeIII GTNAC 3 cut(s) 58, 319, 880
MalI GATC 3 cut(s) 285, 550, 1019
MboI GATC 3 cut(s) 283, 548, 1017
MboII GAAGA 1 cut(s) 970
MfeI CAATTG 1 cut(s) 395
MflI RGATCY 1 cut(s) 283
MhlI GDGCHC 2 cut(s) 637, 1048
MluCI AATT 7 cut(s) 157, 395, 594, 604, 642, 727, 809
MlyI GAGTC 3 cut(s) 98, 249, 980
MmeI TCCRAC 1 cut(s) 237
MnlI CCTC 6 cut(s) 50, 118, 198, 437, 647, 672
Mph1103I ATGCAT 1 cut(s) 931
MroXI GAANNNNTTC 1 cut(s) 859
MseI TTAA 2 cut(s) 786, 1063
MslI CAYNNNNRTG 1 cut(s) 908
MspI CCGG 4 cut(s) 83, 201, 272, 342
MspR9I CCNGG 5 cut(s) 10, 202, 440, 512, 801
MunI CAATTG 1 cut(s) 395
MvaI CCWGG 4 cut(s) 10, 440, 512, 801
MwoI GCNNNNNNNGC 3 cut(s) 391, 899, 930
NciI CCSGG 1 cut(s) 202
NdeI CATATG 1 cut(s) 846
NdeII GATC 3 cut(s) 283, 548, 1017
NlaIII CATG 4 cut(s) 706, 933, 949, 957
NlaIV GGNNCC 3 cut(s) 6, 285, 340
NmuCI GTSAC 3 cut(s) 58, 319, 880
NsiI ATGCAT 1 cut(s) 931
NspI RCATGY 3 cut(s) 933, 949, 957
NspV TTCGAA 1 cut(s) 725
PaeI GCATGC 1 cut(s) 933
PceI AGGCCT 1 cut(s) 859
PdmI GAANNNNTTC 1 cut(s) 859
PfeI GAWTC 1 cut(s) 136
PfoI TCCNGGA 2 cut(s) 510, 799
PkrI GCNGC 2 cut(s) 73, 601
PleI GAGTC 3 cut(s) 98, 249, 980
PmaCI CACGTG 1 cut(s) 484
PmlI CACGTG 1 cut(s) 484
PpsI GAGTC 3 cut(s) 98, 249, 980
Ppu21I YACGTR 1 cut(s) 484
Psp124BI GAGCTC 1 cut(s) 637
Psp6I CCWGG 4 cut(s) 8, 438, 510, 799
PspCI CACGTG 1 cut(s) 484
PspGI CCWGG 4 cut(s) 8, 438, 510, 799
PspN4I GGNNCC 3 cut(s) 6, 285, 340
PsuI RGATCY 1 cut(s) 283
RseI CAYNNNNRTG 1 cut(s) 908
SacI GAGCTC 1 cut(s) 637
SaqAI TTAA 2 cut(s) 786, 1063
SatI GCNGC 2 cut(s) 72, 600
Sau3AI GATC 3 cut(s) 283, 548, 1017
SchI GAGTC 3 cut(s) 98, 249, 980
ScrFI CCNGG 5 cut(s) 10, 202, 440, 512, 801
SduI GDGCHC 2 cut(s) 637, 1048
SfaNI GCATC 2 cut(s) 106, 952
SfcI CTRYAG 1 cut(s) 292
SfuI TTCGAA 1 cut(s) 725
SmiMI CAYNNNNRTG 1 cut(s) 908
SpeI ACTAGT 1 cut(s) 55
SphI GCATGC 1 cut(s) 933
Sse9I AATT 7 cut(s) 157, 395, 594, 604, 642, 727, 809
SseBI AGGCCT 1 cut(s) 859
SsiI CCGC 1 cut(s) 383
SspMI CTAG 2 cut(s) 56, 150
SstI GAGCTC 1 cut(s) 637
StuI AGGCCT 1 cut(s) 859
StyD4I CCNGG 5 cut(s) 8, 200, 438, 510, 799
TaaI ACNGT 4 cut(s) 145, 268, 296, 778
TaiI ACGT 2 cut(s) 486, 1045
TaqI TCGA 4 cut(s) 114, 162, 242, 725
TasI AATT 7 cut(s) 157, 395, 594, 604, 642, 727, 809
TfiI GAWTC 1 cut(s) 136
Tru1I TTAA 2 cut(s) 786, 1063
Tru9I TTAA 2 cut(s) 786, 1063
TscAI CASTG 4 cut(s) 190, 319, 894, 964
TseFI GTSAC 3 cut(s) 58, 319, 880
TseI GCWGC 2 cut(s) 71, 599
Tsp45I GTSAC 3 cut(s) 58, 319, 880
TspDTI ATGAA 3 cut(s) 9, 226, 465
TspGWI ACGGA 3 cut(s) 100, 245, 637
TspRI CASTG 4 cut(s) 190, 319, 894, 964
XapI RAATTY 2 cut(s) 604, 642
XceI RCATGY 3 cut(s) 933, 949, 957
XcmI CCANNNNNNNNNTGG 1 cut(s) 700
XmnI GAANNNNTTC 1 cut(s) 859
XspI CTAG 2 cut(s) 56, 150
Zsp2I ATGCAT 1 cut(s) 931
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.