Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0005883

Physical Location & Seq

Genomic Coordinates Reverse (-)

7334226 .. 7339350

Gene Structure 5125 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT2G16920.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3309 bp

ATGGAGCATGAGCAAGATGACCCTGGTACATCAACAAATGTAGGGGTTGACTCGTCAGTTGATGATTCAATGGCAAGTCTCTCTATATGTGATTCAGAGCATCCTAATATTTATCGACAAGATATTGTCAAAAACAAAAAGACCGGTAGTGTTGGTGTTGTCAGCGAAGTAGCTGGTGATTCTGACTCTGATAGTGATATTAGTGATGAGGAAGAAGATGATGATGATGACGAAGATAATGATGATGATGATGAGGATGTAGAGGAAGGGAAAAAGGCTAGTGAAGAAAATGTTGTTAATGGAGATGGCGAGAAAAAAGCTGATGGGAATTATAAATGTGGTGCACTTGAGGGAGATCAGATTCGTGTGCTTTGGATGGATAACACTGAGCCTGTTCAAGATATTAATGATGTAACTGTTATAGACCGTGGTTTCTTACATGGTGATTATGTTGCTTCTGCTTCTGAGCCTACTGGTCAGGTGGGAGTTGTTGTGGATGTTAACATATCAGTTGATTTGTTGGCTCCTGATGGTTCTATTCACAAGGACATCTCTACCAAGAATTTGAAACGCGTCAGGGATTTTGCTGTTGGTGATTATGTGGTTCATGGTCCATGGCTAGGTAGAATCGATGATGTGTTGGACAATGTGACTGTCTTATTTGATGATGGTTCCATGTGTAAAGTTCTACGTGTTGAACCTCTTCGACTTAAACCTATTCCGAAGAATAATCTTGAAGAAGATGCTAATTTTCCTTATTACCCAGGGCAGCGGGTTAAAGCAAGCTCCTCTTCGGTTTTTAAGAATTCGCGGTGGTTATCTGGACTGTGGAAGCCTAATCGTTTAGAAGGTACGGTGACCAAAGTTACTGCGGGATCAATTTTTGTCTACTGGATTGCCTCAGCTGGCTTTGGTCCAGATTCTTCTGTTTCCCCACCTGAGGAGCAGAATCCAAGTAATTTGACATTGTTGTCATGTTTCACTCATGCCAATTGGCAAGTGGGTGACTGGTGCCTTCTTCCATCTCTGAATCAATCTGCTACAATCCCCTTACACAAGCATGTATCTAAATTAAGACTTTATGATTCCCAAGCTGATCGGCAACAGAAAATTGGGCGTGATTTAGAAGATGTGCAAGATGAAGTAAGTGGAAAAGTTGAACCAGCTGGTATTACTGCTGAAGCTTTGCCAAAAGTAACTAGTGACGATCCTCCTCAAAGAAATCCTTCTGTTTCAAAGGAGCCTGTTCATGAGCCTTGGCCTCTCCATCGGAAGAAGATTCGCAAACTTGTTATCAGAAAGGACAAAAAGGTTAAGAAAAAAGAGGAAAGCTTTGAACAAGCTCTTTTGGTAGTTAATAGCAGAACACGCGTTGATGTATCCTGGCAGGATGGTACAATAGAATGTAGACGGGAAGCGATAACATTGATTCCAATTGAGACTCCTGGTGATCATGAATTTGTCTCTGAGCAGTATGTGGTAGAGAAGACCTCTGATGATGGTGATAACACAACTGAACCTAGACGTGCTGGCGTTGTAAAAAATGTCAATGCAAAAGATCGTACTGCTTCTGTGAGGTGGCTAAATCCACTTCGAAGGGCAGAAGAACCCCGTGAGTTTGAAAAGGAAGAAATTGTCAGTGTGTATGAGCTAGAGGGCCATCCAGATTATGACTACTGTTATGGGGATGTTGTTGTTCGCTTATCACCTATTGCCGTTGCGTTACCAGCATCTTCTCCCGGAAACTCTTTTGAGGAGGCAACACAGCAAGACAATGGATACCAAGATTCAGAATCCCATCAAGAAGCTAAAATCCTTGTGGATAAGGAAGAAAACGAGCCTAGTACGGACCTTTCAAAGCTCTCATGGGTTGGAAATATTACTGGCCTCAAGGATGGTGATATTGAAGTAACATGGGCCGATGGAACAATATCAACGGTTGGCCCTCATGCAGTTTATGTAGTTGGACGCGATGATGATGATGAATCAGTTGCTGGAGAAAGTGAAACAAGTGATGCTGCTAGTTGGGAAACTTTAAACGATGATGATAGGGGTGCTCCTGAGATTCCTGAAGAGGACCTTGGAAGGAGTAGTTCCATTGAGGGAAACTCTGATGCAGATATCTACGCTGAGAATGATTCTGGGAGGAACGGTGCCCTAGCTCTTCCTCTGGCTGCAATCGAATTTGTGACTCGACTGGCTAGTGGAATCTTTTCGCGTGCAAGGAAATCTGTAGATTCTTCCAGTTCTGATTACACGGTTGAAAATGTATATAAGCAAGCTGAATCGACCAACCCGTCCGATGAAACAGATTCCCTTGATGACCCTAGTCCTTCAAAAGTTAATGTGACTGATAATTGCGAGTCAAAGGGCACTCAAGCAAATGCCAAGAATATTTTGAGCGGGGAAACCTCTACATTTTTAGAAGATGAAGATAAGCCAGTACCTTCTGAAGGTGATAGTTGCAGTTTCAGACGCTTTGATATATCACAAGATCCTCTGGACCACCATTTCCTTGGCGTAGATGGGCAGAAAACCAAAGAAAGACAGTGGTTCAAGAAGGTTGATCAAGACTGGAAAATACTTCAGAACAATCTTCCAGATGGAATCTTTGTTCGAGCTTACGAAGATAGAATGGATCTCTTGAGGGCAGTAATAGTCGGGGCATTTGGAACACCATACCAAGATGGTCTCTTCTTTTTTGATTTTCACCTTCCATCTGACTACCCTAGTGTGCCACCGTCGGCATATTATCATTCTGGTGGATGGAGGTTAAACCCCAATCTGTACGAAGAAGGCAAAGTCTGTCTTAGCCTTCTCAATACCTGGACAGGCAGGGGAAATGAAGTCTGGGATCCCAAATCATCTAGCATCCTTCAAGTTCTTGTTTCACTCCAGGGATTGGTGTTGAATTCAAAGCCTTATTTCAATGAAGCCGGGTATGATAAGCAGGTCGGAACTGCGGAAGGAGAAAAAAACTCACTGGGATACAACGAGAATACTTTCTTGCTAAATTGTAAAACCATGATGTATCTTATGCGGAAACCACCAAAGGATTTTGAAGAACTCATCAAAGATCATTTCAGAAAACGCGGATATTACATTTTGAAGGCATGTGACGCCTACATGAAAGGATATCTCATAGGTTCCCTCACCAAAGATGCCTCGGTTATCGATGAACGCAGCAGCGCTAATTCAACTTCAGTTGGTTTTAAGCTTATGCTGGCTAAGATAGCACCAAAGCTTTTCTCAGCACTGAGCGAGGTAGGAGCTGACTGCAATGAATTCCAGCATCTGCAGCAGCAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006464 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019899 GO:0031625 GO:0032446 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044389 GO:0044424 GO:0044444 GO:0044464 GO:0061630 GO:0061659 GO:0070647 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04120 map04120

Pfam Domains

Protein Families

UQ_con

Protein Analysis

1102

Amino Acids

122.18

Weight (kDa)

4.58

Isoelectric Point (pI)

42.07

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SH3-A_UBE2O	PF23048	37 - 142	1.1e-18	UBE2O, N-terminal SH3-A domain
tSH3-B_UBE2O	PF23046	197 - 329	2.8e-44	UBE2O, tandem tSH3-B domain
SH3-B_UBE2O	PF23043	484 - 556	1.2e-19	UBE2O, SH3-B domain
SH3-C_UBE2O	PF23044	611 - 662	5.2e-08	UBE2O, ubiquitin binding SH3-C domain
UQ_con	PF00179	856 - 983	3.4e-19	Ubiquitin-conjugating enzyme

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0005883)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G16920 AT2G16920
fragaria_vesca	FvH4_2g15490 FvH4_2g15490 FvH4_2g15490 FvH4_2g15490 FvH4_2g15490 FvH4_2g15490 FvH4_2g15490
malus_domestica	MD05G1123200.v1.1 MD10G1126200.v1.1
prunus_persica	Prupe.8G167100_v2.0.a1 Prupe.8G167100_v2.0.a1 Prupe.8G167100_v2.0.a1
pyrus_communis	pycom05g11760 pycom10g10840
rosa_chinensis	RchiOBHm_Chr6g0278491
rosa_laevigata	RLG00000013224
rosa_multiflora	Rmu_sc0000258.1_g000006
rosa_roxburghii	Rroxscaffold_7G00189850 Rroxscaffold_7G00190340
rosa_rugosa	Rorug06G0117200 Rorug06G0117200
rosa_samantha	Rh6AG226500 Rh6BG230700 Rh6CG233800 Rh6DG225100
rosa_wichuraiana	Rw6G019770

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	333
AasI	GACNNNNNNGTC	2 cut(s)	970, 2296
Acc36I	ACCTGC	1 cut(s)	2941
AccB1I	GGYRCC	2 cut(s)	1011, 2153
AccB7I	CCANNNNNTGG	1 cut(s)	2902
AccBSI	CCGCTC	1 cut(s)	2403
AccI	GTMKAC	2 cut(s)	888, 1408
AccII	CGCG	6 cut(s)	573, 811, 1371, 1971, 2218, 3093
AciI	CCGC	7 cut(s)	772, 811, 872, 2403, 2963, 3040, 3093
AclWI	GGATC	6 cut(s)	883, 1202, 2489, 2646, 2849, 2862
AcsI	RAATTY	6 cut(s)	562, 805, 1457, 2183, 2911, 3284
AcuI	CTGAAG	5 cut(s)	1200, 2091, 2472, 2570, 3186
AcyI	GRCGYC	1 cut(s)	3120
AfaI	GTAC	7 cut(s)	28, 853, 1396, 1564, 1846, 2445, 2789
AfeI	AGCGCT	1 cut(s)	3190
AfiI	CCNNNNNNNGG	4 cut(s)	620, 940, 2453, 2902
AflIII	ACRYGT	3 cut(s)	571, 691, 1369
AgeI	ACCGGT	1 cut(s)	143
AhlI	ACTAGT	1 cut(s)	1199
AjiI	CACGTC	1 cut(s)	1526
AjnI	CCWGG	6 cut(s)	22, 763, 1382, 1444, 2825, 2895
AjuI	GAANNNNNNNTTGG	2 cut(s)	2064, 2096
AloI	GAACNNNNNNTCC	2 cut(s)	2598, 2630
Alw21I	GWGCWC	2 cut(s)	346, 2059
Alw26I	GTCTC	4 cut(s)	83, 1433, 1468, 2696
Alw44I	GTGCAC	1 cut(s)	342
AlwI	GGATC	6 cut(s)	883, 1202, 2489, 2646, 2849, 2862
AlwNI	CAGNNNCTG	2 cut(s)	1994, 3295
Aor51HI	AGCGCT	1 cut(s)	3190
AoxI	GGCC	5 cut(s)	1259, 1657, 1885, 1917, 1942
ApaLI	GTGCAC	1 cut(s)	342
ApeKI	GCWGC	7 cut(s)	769, 2018, 2174, 3183, 3186, 3298, 3301
ApoI	RAATTY	6 cut(s)	562, 805, 1457, 2183, 2911, 3284
AseI	ATTAAT	1 cut(s)	405
AsiGI	ACCGGT	1 cut(s)	143
Asp700I	GAANNNNTTC	4 cut(s)	702, 1225, 2212, 3002
AspLEI	GCGC	1 cut(s)	3191
AspS9I	GGNCC	8 cut(s)	611, 914, 1657, 1849, 1917, 1943, 2077, 2503
AsuC2I	CCSGG	2 cut(s)	1740, 2938
AsuII	TTCGAA	1 cut(s)	1594
AvaII	GGWCC	5 cut(s)	611, 914, 1849, 2077, 2503
AxyI	CCTNAGG	1 cut(s)	939
BaeGI	GKGCMC	3 cut(s)	346, 2158, 2375
BamHI	GGATCC	1 cut(s)	2854
BanI	GGYRCC	2 cut(s)	1011, 2153
BbsI	GAAGAC	1 cut(s)	1493
Bbv12I	GWGCWC	2 cut(s)	346, 2059
BbvCI	CCTCAGC	1 cut(s)	901
BbvI	GCAGC	5 cut(s)	781, 2005, 2161, 3195, 3198
BceAI	ACGGC	1 cut(s)	1700
BciT130I	CCWGG	6 cut(s)	24, 765, 1384, 1446, 2827, 2897
BciVI	GTATCC	3 cut(s)	1390, 1772, 2981
BclI	TGATCA	2 cut(s)	1450, 2566
BcnI	CCSGG	2 cut(s)	1740, 2938
BcoDI	GTCTC	4 cut(s)	83, 1433, 1468, 2696
BcuI	ACTAGT	1 cut(s)	1199
BfmI	CTRYAG	2 cut(s)	2232, 3296
BfoI	RGCGCY	1 cut(s)	3192
BfuAI	ACCTGC	1 cut(s)	2941
BfuI	GTATCC	3 cut(s)	1390, 1772, 2981
BisI	GCNGC	7 cut(s)	770, 2019, 2175, 3184, 3187, 3299, 3302
BlsI	GCNGC	7 cut(s)	771, 2020, 2176, 3185, 3188, 3300, 3303
Bme1390I	CCNGG	8 cut(s)	24, 765, 1384, 1446, 1740, 2827, 2897, 2938
Bme18I	GGWCC	5 cut(s)	611, 914, 1849, 2077, 2503
BmgBI	CACGTC	1 cut(s)	1526
BmgT120I	GGNCC	8 cut(s)	611, 914, 1657, 1849, 1917, 1943, 2077, 2503
BmiI	GGNNCC	7 cut(s)	525, 673, 1013, 1242, 2155, 2856, 3148
BmrFI	CCNGG	8 cut(s)	24, 765, 1384, 1446, 1740, 2827, 2897, 2938
BmrI	ACTGGG	1 cut(s)	2993
BmsI	GCATC	8 cut(s)	109, 733, 1739, 2005, 2104, 2880, 3151, 3301
BmuI	ACTGGG	1 cut(s)	2993
BoxI	GACNNNNGTC	1 cut(s)	2328
BpiI	GAAGAC	1 cut(s)	1493
BplI	GAGNNNNNCTC	4 cut(s)	1475, 1507, 2093, 2125
BpmI	CTGGAG	2 cut(s)	2016, 2879
Bpu10I	CCTNAGC	1 cut(s)	901
Bpu14I	TTCGAA	1 cut(s)	1594
BpuEI	CTTGAG	4 cut(s)	368, 1874, 2361, 2665
BpuMI	CCSGG	2 cut(s)	1740, 2938
Bsa29I	ATCGAT	2 cut(s)	630, 3174
BsaAI	YACGTR	1 cut(s)	692
BsaHI	GRCGYC	1 cut(s)	3120
BsaI	GGTCTC	1 cut(s)	2696
BsaWI	WCCGGW	1 cut(s)	143
BsaXI	ACNNNNNCTCC	4 cut(s)	477, 507, 2137, 2167
Bsc4I	CCNNNNNNNGG	4 cut(s)	620, 940, 2453, 2902
Bse118I	RCCGGY	1 cut(s)	143
Bse1I	ACTGG	9 cut(s)	478, 896, 1013, 1888, 2202, 2244, 2441, 2579, 2988
Bse21I	CCTNAGG	1 cut(s)	939
Bse3DI	GCAATG	1 cut(s)	3286
BseBI	CCWGG	6 cut(s)	24, 765, 1384, 1446, 2827, 2897
BseCI	ATCGAT	2 cut(s)	630, 3174
BseLI	CCNNNNNNNGG	4 cut(s)	620, 940, 2453, 2902
BseMI	GCAATG	1 cut(s)	3286
BseMII	CTCAG	9 cut(s)	378, 456, 915, 930, 1458, 2052, 2121, 3248, 3264
BseNI	ACTGG	9 cut(s)	478, 896, 1013, 1888, 2202, 2244, 2441, 2579, 2988
BseRI	GAGGAG	4 cut(s)	778, 956, 1203, 1769
BseSI	GKGCMC	3 cut(s)	346, 2158, 2375
BseXI	GCAGC	5 cut(s)	781, 2005, 2161, 3195, 3198
Bsh1236I	CGCG	6 cut(s)	573, 811, 1371, 1971, 2218, 3093
BshFI	GGCC	5 cut(s)	1261, 1659, 1887, 1919, 1944
BshNI	GGYRCC	2 cut(s)	1011, 2153
BshTI	ACCGGT	1 cut(s)	143
BshVI	ATCGAT	2 cut(s)	630, 3174
BsiHKAI	GWGCWC	2 cut(s)	346, 2059
BsiSI	CCGG	3 cut(s)	144, 1740, 2937
BslI	CCNNNNNNNGG	4 cut(s)	620, 940, 2453, 2902
BsmAI	GTCTC	4 cut(s)	83, 1433, 1468, 2696
BsnI	GGCC	5 cut(s)	1261, 1659, 1887, 1919, 1944
Bso31I	GGTCTC	1 cut(s)	2696
Bsp119I	TTCGAA	1 cut(s)	1594
Bsp1286I	GDGCHC	4 cut(s)	346, 2059, 2158, 2375
Bsp19I	CCATGG	1 cut(s)	614
BspACI	CCGC	7 cut(s)	772, 811, 872, 2403, 2963, 3040, 3093
BspANI	GGCC	5 cut(s)	1261, 1659, 1887, 1919, 1944
BspCNI	CTCAG	9 cut(s)	379, 457, 914, 931, 1459, 2053, 2122, 3249, 3263
BspDI	ATCGAT	2 cut(s)	630, 3174
BspFNI	CGCG	6 cut(s)	573, 811, 1371, 1971, 2218, 3093
BspHI	TCATGA	2 cut(s)	1249, 1453
BspLI	GGNNCC	7 cut(s)	525, 673, 1013, 1242, 2155, 2856, 3148
BspMAI	CTGCAG	1 cut(s)	3300
BspMI	ACCTGC	1 cut(s)	2941
BspPI	GGATC	6 cut(s)	883, 1202, 2489, 2646, 2849, 2862
BspQI	GCTCTTC	1 cut(s)	2169
BspT104I	TTCGAA	1 cut(s)	1594
BspT107I	GGYRCC	2 cut(s)	1011, 2153
BspTNI	GGTCTC	1 cut(s)	2696
BsrBI	CCGCTC	1 cut(s)	2403
BsrDI	GCAATG	1 cut(s)	3286
BsrFI	RCCGGY	1 cut(s)	143
BsrI	ACTGG	9 cut(s)	478, 896, 1013, 1888, 2202, 2244, 2441, 2579, 2988
BssAI	RCCGGY	1 cut(s)	143
BssNI	GRCGYC	1 cut(s)	3120
BssT1I	CCWWGG	4 cut(s)	614, 1256, 2080, 2515
Bst2UI	CCWGG	6 cut(s)	24, 765, 1384, 1446, 2827, 2897
Bst6I	CTCTTC	5 cut(s)	708, 796, 2067, 2169, 2699
BstACI	GRCGYC	1 cut(s)	3120
BstBAI	YACGTR	1 cut(s)	692
BstBI	TTCGAA	1 cut(s)	1594
BstC8I	GCNNGC	6 cut(s)	784, 907, 1531, 2220, 2280, 3225
BstDSI	CCRYGG	2 cut(s)	427, 614
BstEII	GGTNACC	1 cut(s)	856
BstENI	CCTNNNNNAGG	1 cut(s)	2451
BstFNI	CGCG	6 cut(s)	573, 811, 1371, 1971, 2218, 3093
BstH2I	RGCGCY	1 cut(s)	3192
BstHHI	GCGC	1 cut(s)	3191
BstMAI	GTCTC	4 cut(s)	83, 1433, 1468, 2696
BstMWI	GCNNNNNNNGC	5 cut(s)	1368, 1727, 3119, 3233, 3298
BstNI	CCWGG	6 cut(s)	24, 765, 1384, 1446, 2827, 2897
BstNSI	RCATGY	2 cut(s)	1064, 3117
BstPAI	GACNNNNGTC	1 cut(s)	2328
BstPI	GGTNACC	1 cut(s)	856
BstSCI	CCNGG	8 cut(s)	22, 763, 1382, 1444, 1738, 2825, 2895, 2936
BstSFI	CTRYAG	2 cut(s)	2232, 3296
BstSLI	GKGCMC	3 cut(s)	346, 2158, 2375
BstUI	CGCG	6 cut(s)	573, 811, 1371, 1971, 2218, 3093
BstV1I	GCAGC	5 cut(s)	781, 2005, 2161, 3195, 3198
BstV2I	GAAGAC	1 cut(s)	1493
BstX2I	RGATCY	3 cut(s)	2494, 2638, 2854
BstXI	CCANNNNNNTGG	1 cut(s)	2516
BstYI	RGATCY	3 cut(s)	2494, 2638, 2854
Bsu15I	ATCGAT	2 cut(s)	630, 3174
Bsu36I	CCTNAGG	1 cut(s)	939
BsuI	GTATCC	3 cut(s)	1390, 1772, 2981
BsuRI	GGCC	5 cut(s)	1261, 1659, 1887, 1919, 1944
BsuTUI	ATCGAT	2 cut(s)	630, 3174
BtgI	CCRYGG	2 cut(s)	427, 614
BtgZI	GCGATG	1 cut(s)	1986
BtrI	CACGTC	1 cut(s)	1526
BtsIMutI	CAGTG	5 cut(s)	384, 1645, 2555, 2981, 3254
BveI	ACCTGC	1 cut(s)	2941
Cac8I	GCNNGC	6 cut(s)	784, 907, 1531, 2220, 2280, 3225
CaiI	CAGNNNCTG	2 cut(s)	1994, 3295
CciI	TCATGA	2 cut(s)	1249, 1453
CfoI	GCGC	1 cut(s)	3191
Cfr10I	RCCGGY	1 cut(s)	143
Cfr13I	GGNCC	8 cut(s)	611, 914, 1657, 1849, 1917, 1943, 2077, 2503
ClaI	ATCGAT	2 cut(s)	630, 3174
CseI	GACGC	4 cut(s)	562, 1977, 2484, 3128
Csp6I	GTAC	7 cut(s)	27, 852, 1395, 1563, 1845, 2444, 2788
CspAI	ACCGGT	1 cut(s)	143
CviQI	GTAC	7 cut(s)	27, 852, 1395, 1563, 1845, 2444, 2788
DraI	TTTAAA	1 cut(s)	2037
DrdI	GACNNNNNNGTC	2 cut(s)	970, 2296
DseDI	GACNNNNNNGTC	2 cut(s)	970, 2296
Eam1104I	CTCTTC	5 cut(s)	708, 796, 2067, 2169, 2699
EarI	CTCTTC	5 cut(s)	708, 796, 2067, 2169, 2699
Eco130I	CCWWGG	4 cut(s)	614, 1256, 2080, 2515
Eco31I	GGTCTC	1 cut(s)	2696
Eco32I	GATATC	2 cut(s)	2122, 3137
Eco47I	GGWCC	5 cut(s)	611, 914, 1849, 2077, 2503
Eco47III	AGCGCT	1 cut(s)	3190
Eco57I	CTGAAG	5 cut(s)	1200, 2091, 2472, 2570, 3186
Eco81I	CCTNAGG	1 cut(s)	939
Eco91I	GGTNACC	1 cut(s)	856
EcoNI	CCTNNNNNAGG	1 cut(s)	2451
EcoO109I	RGGNCCY	1 cut(s)	2077
EcoO65I	GGTNACC	1 cut(s)	856
EcoRI	GAATTC	3 cut(s)	805, 2911, 3284
EcoRII	CCWGG	6 cut(s)	22, 763, 1382, 1444, 2825, 2895
EcoRV	GATATC	2 cut(s)	2122, 3137
EcoT14I	CCWWGG	4 cut(s)	614, 1256, 2080, 2515
ErhI	CCWWGG	4 cut(s)	614, 1256, 2080, 2515
FalI	AAGNNNNNCTT	8 cut(s)	775, 807, 1210, 1242, 2064, 2096, 2793, 2825
FauI	CCCGC	3 cut(s)	765, 865, 2396
FbaI	TGATCA	2 cut(s)	1450, 2566
FblI	GTMKAC	2 cut(s)	888, 1408
Fnu4HI	GCNGC	7 cut(s)	770, 2019, 2175, 3184, 3187, 3299, 3302
Fsp4HI	GCNGC	7 cut(s)	770, 2019, 2175, 3184, 3187, 3299, 3302
GlaI	GCGC	1 cut(s)	3190
GluI	GCNGC	7 cut(s)	770, 2019, 2175, 3184, 3187, 3299, 3302
GsuI	CTGGAG	2 cut(s)	2016, 2879
HaeII	RGCGCY	1 cut(s)	3192
HaeIII	GGCC	5 cut(s)	1261, 1659, 1887, 1919, 1944
HapII	CCGG	3 cut(s)	144, 1740, 2937
HgaI	GACGC	4 cut(s)	562, 1977, 2484, 3128
HhaI	GCGC	1 cut(s)	3191
Hin1I	GRCGYC	1 cut(s)	3120
Hin6I	GCGC	1 cut(s)	3189
HinP1I	GCGC	1 cut(s)	3189
HincII	GTYRAC	2 cut(s)	49, 502
HindII	GTYRAC	2 cut(s)	49, 502
HindIII	AAGCTT	4 cut(s)	1182, 1330, 3215, 3242
HpaI	GTTAAC	1 cut(s)	502
HpaII	CCGG	3 cut(s)	144, 1740, 2937
Hpy166II	GTNNAC	5 cut(s)	49, 344, 502, 889, 1409
Hpy8I	GTNNAC	5 cut(s)	49, 344, 502, 889, 1409
Hpy99I	CGWCG	1 cut(s)	2746
HpyCH4IV	ACGT	2 cut(s)	691, 1525
HpyF10VI	GCNNNNNNNGC	5 cut(s)	1368, 1727, 3119, 3233, 3298
HpySE526I	ACGT	2 cut(s)	691, 1525
Hsp92I	GRCGYC	1 cut(s)	3120
HspAI	GCGC	1 cut(s)	3189
Ksp22I	TGATCA	2 cut(s)	1450, 2566
KspAI	GTTAAC	1 cut(s)	502
LguI	GCTCTTC	1 cut(s)	2169
LmnI	GCTCC	7 cut(s)	4, 529, 791, 943, 1240, 2062, 3269
Lsp1109I	GCAGC	5 cut(s)	781, 2005, 2161, 3195, 3198
LweI	GCATC	8 cut(s)	109, 733, 1739, 2005, 2104, 2880, 3151, 3301
MaeII	ACGT	2 cut(s)	691, 1525
MbiI	CCGCTC	1 cut(s)	2403
MfeI	CAATTG	2 cut(s)	991, 1434
MflI	RGATCY	3 cut(s)	2494, 2638, 2854
MhlI	GDGCHC	4 cut(s)	346, 2059, 2158, 2375
MluI	ACGCGT	2 cut(s)	571, 1369
MlyI	GAGTC	5 cut(s)	44, 179, 1435, 2185, 2372
MmeI	TCCRAC	4 cut(s)	621, 1852, 1945, 2935
MroXI	GAANNNNTTC	4 cut(s)	702, 1225, 2212, 3002
MslI	CAYNNNNRTG	2 cut(s)	1059, 2760
MspA1I	CMGCKG	3 cut(s)	772, 905, 1166
MspI	CCGG	3 cut(s)	144, 1740, 2937
MspR9I	CCNGG	8 cut(s)	24, 765, 1384, 1446, 1740, 2827, 2897, 2938
MunI	CAATTG	2 cut(s)	991, 1434
MvaI	CCWGG	6 cut(s)	24, 765, 1384, 1446, 2827, 2897
MvnI	CGCG	6 cut(s)	573, 811, 1371, 1971, 2218, 3093
MwoI	GCNNNNNNNGC	5 cut(s)	1368, 1727, 3119, 3233, 3298
NciI	CCSGG	2 cut(s)	1740, 2938
NcoI	CCATGG	1 cut(s)	614
NlaIV	GGNNCC	7 cut(s)	525, 673, 1013, 1242, 2155, 2856, 3148
NmuCI	GTSAC	7 cut(s)	649, 856, 1004, 1202, 2188, 2347, 3116
NspI	RCATGY	2 cut(s)	1064, 3117
NspV	TTCGAA	1 cut(s)	1594
PagI	TCATGA	2 cut(s)	1249, 1453
PasI	CCCWGGG	1 cut(s)	764
PciSI	GCTCTTC	1 cut(s)	2169
PcsI	WCGNNNNNNNCGW	1 cut(s)	2294
PdmI	GAANNNNTTC	4 cut(s)	702, 1225, 2212, 3002
PflMI	CCANNNNNTGG	1 cut(s)	2902
PfoI	TCCNGGA	1 cut(s)	1738
PinAI	ACCGGT	1 cut(s)	143
PkrI	GCNGC	7 cut(s)	771, 2020, 2176, 3185, 3188, 3300, 3303
PleI	GAGTC	5 cut(s)	44, 179, 1435, 2185, 2371
PpsI	GAGTC	5 cut(s)	44, 179, 1435, 2185, 2371
Ppu21I	YACGTR	1 cut(s)	692
PpuMI	RGGWCCY	1 cut(s)	2077
PshAI	GACNNNNGTC	1 cut(s)	2328
PshBI	ATTAAT	1 cut(s)	405
PsiI	TTATAA	1 cut(s)	333
Psp5II	RGGWCCY	1 cut(s)	2077
Psp6I	CCWGG	6 cut(s)	22, 763, 1382, 1444, 2825, 2895
PspEI	GGTNACC	1 cut(s)	856
PspGI	CCWGG	6 cut(s)	22, 763, 1382, 1444, 2825, 2895
PspN4I	GGNNCC	7 cut(s)	525, 673, 1013, 1242, 2155, 2856, 3148
PspPI	GGNCC	8 cut(s)	611, 914, 1657, 1849, 1917, 1943, 2077, 2503
PspPPI	RGGWCCY	1 cut(s)	2077
PstI	CTGCAG	1 cut(s)	3300
PstNI	CAGNNNCTG	2 cut(s)	1994, 3295
PsuI	RGATCY	3 cut(s)	2494, 2638, 2854
PvuII	CAGCTG	2 cut(s)	905, 1166
RsaI	GTAC	7 cut(s)	28, 853, 1396, 1564, 1846, 2445, 2789
RsaNI	GTAC	7 cut(s)	27, 852, 1395, 1563, 1845, 2444, 2788
RseI	CAYNNNNRTG	2 cut(s)	1059, 2760
SapI	GCTCTTC	1 cut(s)	2169
SatI	GCNGC	7 cut(s)	770, 2019, 2175, 3184, 3187, 3299, 3302
Sau96I	GGNCC	8 cut(s)	611, 914, 1657, 1849, 1917, 1943, 2077, 2503
SchI	GAGTC	5 cut(s)	44, 179, 1435, 2185, 2372
ScrFI	CCNGG	8 cut(s)	24, 765, 1384, 1446, 1740, 2827, 2897, 2938
SduI	GDGCHC	4 cut(s)	346, 2059, 2158, 2375
SfaNI	GCATC	8 cut(s)	109, 733, 1739, 2005, 2104, 2880, 3151, 3301
SfcI	CTRYAG	2 cut(s)	2232, 3296
SfuI	TTCGAA	1 cut(s)	1594
SinI	GGWCC	5 cut(s)	611, 914, 1849, 2077, 2503
SmiMI	CAYNNNNRTG	2 cut(s)	1059, 2760
SmlI	CTYRAG	4 cut(s)	347, 1889, 2376, 2644
SmoI	CTYRAG	4 cut(s)	347, 1889, 2376, 2644
SpeI	ACTAGT	1 cut(s)	1199
SsiI	CCGC	7 cut(s)	772, 811, 872, 2403, 2963, 3040, 3093
SspI	AATATT	3 cut(s)	109, 1879, 2395
StyD4I	CCNGG	8 cut(s)	22, 763, 1382, 1444, 1738, 2825, 2895, 2936
StyI	CCWWGG	4 cut(s)	614, 1256, 2080, 2515
TaiI	ACGT	2 cut(s)	694, 1528
TaqI	TCGA	9 cut(s)	115, 630, 706, 1594, 2181, 2194, 2288, 2617, 3174
TscAI	CASTG	5 cut(s)	391, 1645, 2555, 2988, 3261
TseFI	GTSAC	7 cut(s)	649, 856, 1004, 1202, 2188, 2347, 3116
TseI	GCWGC	7 cut(s)	769, 2018, 2174, 3183, 3186, 3298, 3301
Tsp45I	GTSAC	7 cut(s)	649, 856, 1004, 1202, 2188, 2347, 3116
TspGWI	ACGGA	1 cut(s)	1862
TspRI	CASTG	5 cut(s)	391, 1645, 2555, 2988, 3261
Van91I	CCANNNNNTGG	1 cut(s)	2902
VneI	GTGCAC	1 cut(s)	342
VpaK11BI	GGWCC	5 cut(s)	611, 914, 1849, 2077, 2503
VspI	ATTAAT	1 cut(s)	405
XagI	CCTNNNNNAGG	1 cut(s)	2451
XapI	RAATTY	6 cut(s)	562, 805, 1457, 2183, 2911, 3284
XceI	RCATGY	2 cut(s)	1064, 3117
XcmI	CCANNNNNNNNNTGG	2 cut(s)	997, 2513
XmiI	GTMKAC	2 cut(s)	888, 1408
XmnI	GAANNNNTTC	4 cut(s)	702, 1225, 2212, 3002

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.