Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0005883

Physical Location & Seq

Genomic Coordinates Reverse (-)

24575205 .. 24582544

Gene Structure 7340 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1123200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3444 bp

ATGGGAACCAAGGAGCATGACACTGTTTTTAAAGATGACGAGCCTACTGCTATTGACCACGATAATATTTCGTTGAGCCGGGTTGATTCTTCAACAAGCACAGTTCTTTGCGATTCAAATGTAAATACTGAAAGTAATGAAGTTAAAAAGTTAAGTGAGACTGTCAGTAACATTAACAATACCCCATATATTTATAGACAAGATGTCGTCAGGAGCAAGAGTGGTATGACTGGGATTGTGACTGAGGTAGCTGGTGATTCAGATTCTGATAGTAGCATCACTGATGACGAGGATGACGATGAAGATGATGACTACGACGATGATGAGAATGAGGAAGAAGGTGGTAATAACAATAATACCACTCATGGGAATGGTGACAAGAACAAAAATGGTAGTAATGATAAGACTGGTCCCCTTCCCGCTGACCAGGTTCGAGTACTTTGGACGGATGAGACTGAGTCAACCCAAAACATTAATGATTTAACAGTTGTTGATCGAGGGTTCTTACATGGGGATTTTGTTGCTGCTGCTTCTGACCCAACCGGGCAAGTTGGTGTTGTGGTGGATGTCAATATCTCTGTTGATCTATTAGCTCCTGACGGGTCAGTCATAAAAGACCTTTCATCCAACGAATTAAAACGTGTGAGGGAGTTCACCGTGGGTGATTATGTGGTCCTTGGTCCCTGGCTAGGTAGGATTGATGATGTTTATGATAATGTGACAGTTTTGTTTGATGATGGTTCTGTGTGTAAAATCATGAAAGCAGAGCCAATGGATCTGAAACCTGTTGCCAAGAATATCCTTGAGGATGTGCATTTCCCTTACTACCCAGGGCAGCGTGTGAAGGCAAGATCGTCAGTTTTCAAGAATGCTAGGTGGCTATCTGGCTTGTGGAAACCTAATCGTGTGGAAGGTACTGTTACTAAGGTTACAGTTGCTTCAGTATTAATTTATTGGATAGCTTCTGCTGGCTGTGGGCCAGATTCTTCTACTGCACCTGCTAAAGAGCAGATTCCCAAGAACTTGAAATTATTGTCTTGTTTTACACATGCAACTTGGCAATTGGGTGATTGGTGTCTTCTTCCCTCATCAGTGTCATCATCTTCCATTCCGTTAGACAAGGGATCATCAAATTTAGAACTTCATGATTCTGTAAGCAGTGACTTAGAACCTACTCAAATGGGTAGTAAGTGTGATTCAGAAGAGAGTGCGCTAGATGAACCAAATGGCAATCACGAATCCATGGATATCGATCCAGTATCTGTATTAGATGGAAACAATGGAAATACTGGAAGGAATACATCTGTTGAATCTAGTTTGTGTAATAGTTTATTGTCTGTTTCAAAGGAGCCTGTTCATGAAACCTGGCCTCTTCATCGCAAAAAGATCCGCAAAGTTGTGGTTAGGAGAGACAAGAAGGCACGGAAGGAAGAAAATTTTGAAAGATCCTTTTTGATTGTCAATACCAAGACGACAGTTGATGTGGCATGGCAGGATGGTACAACAGAACTGAAACTGGACTCAAAAGATTTAATTCCGCTTGATAGTCCGGGTGATCACGAATTTGTTGCTGAACAGTATGTGGTGGAGAAGGCGTCTGATGATGGTGATGATGCTTATGAAGCTAGGCGTGTTGGGCTTGTGAAAAGTGTCAATGCCAAGGAGAGAACAGCTTGTGTGAGGTGGTTAAAGCCAGTTTCCAGACCGGAAGATCCTAGAGAGTTTGAGAAGGAGGAAGTTGTGAGTGTCTATGAGCTTGAGGGGCATCCGGATTATGATTACTGTTATGGGGATGTTGTTGTCAGATTGTTGCCAGTTTTTGTCTCTGCACAAACTGCTTCAGGGACAGACTTAGATGAGGAACCAAAGCAACAAAACGGACCAAGTGATCTTAGTTCCACTTCTACAAAAAAGGAAGATCTATCCAGTGATGAAGCTTGTTTGGACTTCTCTGATCTTTCTTGGGTTGGAAATATAACTGGTCTTAAGAATGGGGATATTGAAGTTACATGGGCTGACGGGATGGTTTCTACGGTTGGACCTCAAGCAATTTATGTTGTTGGCCGAGCTGATGATGATGACTCCATCGGTGCTGGAAGTGATGTTAGTGATGCTGCTAGTTGGGAAACGGTTAATGATGATGAGATGCCTGCACTATTTGCCCCTGCGAGTACAGAAGAGGAAGTTCAGCTACAAAATGCCATTGGGATGAATTTTGAAGCTGAAGAGAGTGGAGAGAATAATTCTGGGAGGAACCCGGCATTGGCTGTTCCCTTGGCTGCACTTAAGTTCGTTACCAGACTGGCACATGGTATATTCTCAAGAGGACAAAGGAATTTGGATTCAAATTCTTTGGATACTGAAGGTGAGGGCGAAGGTGAAGTTGAGCCTCGGGAAGTGGAAATTGCTCAGGGAAGAGATCATGGTGAAGATTCCAGCTCTCAGAAATCTAATGTGGTGGATCCTTATGGTCTAGAAATAAATAAGGAAGAAGAGAAACATGTTACCCCACAGGCTACAGAAATGTTAGATGCAGCAGAAATTCTATGCAACTTAAAGACTGAAGAATCAGATTCTATAGAATGCAGTAAGGATGACGCTTGCAGTTTCAAACGCTTTGATATTGCCAAAGATCCTTTGGACCATCATTTCCTTTGTGCAGCCGGGCAGAATACCAGTGGAAGAAAGTGGTTTAAGAAGGTTCAGCAAGATTGGAACATCCTTCAAAATAACCTTCCTGATGGGATTTGTGTACGAGTTTATGAAGATCGAATGGATCTCTTGAGGGCAGTTATAGTTGGGGCTTATGGGACACCTTACCAAGATGGCCTCTTCTTTTTTGATTTTCACCTCCCTCCAGAGTACCCTGATGTTCCTCTGACAGCATACTATCATTCTGGTGGCTGGCGGATAAATCCAAATCTATATGAAGAAGGCAAAGTGTGTCTTAGTCTTTTGAATACTTGGACTGGCAGAGGGAATGAAGTATGGGATCCAAAGTCTTCTAGCATCCTTCAAGTTCTTGTTTCGCTCCAAGGGTTGGTGCTAAATTCTAAGCCATATTTTAATGAAGCGGGCTACGATAAGCAGGTTGGGACGGCTGAAGGAGAGAAAAATTCATTGTCCTACAATGAGAATACTTTCTTACTGAACTGCAAGACAATGATGTATCTTATTCGGAGGCCACCAAAGGACTTTGAAGAGCTCGTCAATGACCATTTCCGGAGGCGTGGTTTTTATATCTTGAAGGCCTGCGATGCATACATGAAAGGCTATTCGATTGGTTCTCTCACTAAAGATGCCTCTCTTAGTGATAAGAGTGATGTAAATTCAACTTCGGTTGGTTTCAAGTTGATGTTAGCTAAGATTGTGCCAAAGCTTTTCTCGGCCCTGAGTGAGGTTGGAGCTAATTGTCAAGAATTTAAGCATCTGCAACAATCGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006464 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019899 GO:0031625 GO:0032446 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044389 GO:0044424 GO:0044444 GO:0044464 GO:0061630 GO:0061659 GO:0070647 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04120 map04120

Pfam Domains

Protein Families

UQ_con

Protein Analysis

1148

Amino Acids

126.59

Weight (kDa)

4.54

Isoelectric Point (pI)

38.32

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SH3-A_UBE2O	PF23048	64 - 164	1.3e-11	UBE2O, N-terminal SH3-A domain
tSH3-B_UBE2O	PF23046	220 - 350	5e-46	UBE2O, tandem tSH3-B domain
SH3-B_UBE2O	PF23043	519 - 591	2.4e-20	UBE2O, SH3-B domain
SH3-C_UBE2O	PF23044	653 - 693	2.3e-06	UBE2O, ubiquitin binding SH3-C domain
UQ_con	PF00179	901 - 1031	4e-18	Ubiquitin-conjugating enzyme

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0005883)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G16920 AT2G16920
fragaria_vesca	FvH4_2g15490 FvH4_2g15490 FvH4_2g15490 FvH4_2g15490 FvH4_2g15490 FvH4_2g15490 FvH4_2g15490
malus_domestica	MD05G1123200.v1.1 MD10G1126200.v1.1
prunus_persica	Prupe.8G167100_v2.0.a1 Prupe.8G167100_v2.0.a1 Prupe.8G167100_v2.0.a1
pyrus_communis	pycom05g11760 pycom10g10840
rosa_chinensis	RchiOBHm_Chr6g0278491
rosa_laevigata	RLG00000013224
rosa_multiflora	Rmu_sc0000258.1_g000006
rosa_roxburghii	Rroxscaffold_7G00189850 Rroxscaffold_7G00190340
rosa_rugosa	Rorug06G0117200 Rorug06G0117200
rosa_samantha	Rh6AG226500 Rh6BG230700 Rh6CG233800 Rh6DG225100
rosa_wichuraiana	Rw6G019770

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1006
AasI	GACNNNNNNGTC	1 cut(s)	605
Acc36I	ACCTGC	2 cut(s)	1006, 3079
AccB7I	CCANNNNNTGG	1 cut(s)	3040
AccIII	TCCGGA	2 cut(s)	1768, 3222
AciI	CCGC	5 cut(s)	420, 1390, 1538, 2908, 3074
AcoI	YGGCCR	1 cut(s)	2062
AcuI	CTGAAG	6 cut(s)	924, 1824, 2244, 2382, 2583, 3123
AcyI	GRCGYC	1 cut(s)	1595
AfaI	GTAC	6 cut(s)	438, 916, 1501, 2173, 2754, 2864
AfiI	CCNNNNNNNGG	5 cut(s)	366, 689, 2263, 3040, 3397
AflII	CTTAAG	2 cut(s)	1985, 2285
AflIII	ACRYGT	2 cut(s)	640, 2500
AhdI	GACNNNNNGTC	1 cut(s)	203
AjnI	CCWGG	4 cut(s)	426, 683, 829, 1364
AjuI	GAANNNNNNNTTGG	2 cut(s)	2246, 2278
Alw21I	GWGCWC	1 cut(s)	3207
Alw26I	GTCTC	4 cut(s)	152, 446, 1404, 1828
AlwNI	CAGNNNCTG	2 cut(s)	266, 1262
Ama87I	CYCGRG	1 cut(s)	2391
Aor13HI	TCCGGA	2 cut(s)	1768, 3222
AoxI	GGCC	7 cut(s)	977, 1367, 2062, 2827, 3182, 3249, 3387
ApeKI	GCWGC	7 cut(s)	524, 527, 835, 2114, 2279, 2534, 2660
AseI	ATTAAT	2 cut(s)	474, 947
Asp700I	GAANNNNTTC	1 cut(s)	3140
AspLEI	GCGC	1 cut(s)	1213
AspS9I	GGNCC	8 cut(s)	410, 673, 680, 977, 1880, 2039, 2641, 3388
AsuC2I	CCSGG	5 cut(s)	80, 544, 1551, 2258, 2665
AvaI	CYCGRG	1 cut(s)	2391
AvaII	GGWCC	6 cut(s)	410, 673, 680, 1880, 2039, 2641
BamHI	GGATCC	2 cut(s)	2461, 2992
BanII	GRGCYC	1 cut(s)	3207
BarI	GAAGNNNNNNTAC	2 cut(s)	2175, 2207
BbsI	GAAGAC	2 cut(s)	1070, 2994
Bbv12I	GWGCWC	1 cut(s)	3207
BbvI	GCAGC	7 cut(s)	511, 514, 847, 2101, 2266, 2546, 2672
BccI	CCATC	9 cut(s)	731, 1265, 1490, 1598, 2017, 2093, 2652, 2735, 2819
BceAI	ACGGC	1 cut(s)	3114
BcgI	CGANNNNNNTGC	4 cut(s)	29, 63, 1550, 1584
BciT130I	CCWGG	4 cut(s)	428, 685, 831, 1366
BciVI	GTATCC	1 cut(s)	2350
BclI	TGATCA	1 cut(s)	1555
BcnI	CCSGG	5 cut(s)	80, 544, 1551, 2258, 2665
BcoDI	GTCTC	4 cut(s)	152, 446, 1404, 1828
BfaI	CTAG	9 cut(s)	689, 873, 1214, 1314, 1626, 1716, 2118, 2474, 3006
BfmI	CTRYAG	2 cut(s)	2517, 2577
BfrI	CTTAAG	2 cut(s)	1985, 2285
BfuAI	ACCTGC	2 cut(s)	1006, 3079
BfuI	GTATCC	1 cut(s)	2350
BglII	AGATCT	1 cut(s)	1918
BisI	GCNGC	7 cut(s)	525, 528, 836, 2115, 2280, 2535, 2661
BlsI	GCNGC	7 cut(s)	526, 529, 837, 2116, 2281, 2536, 2662
BmcAI	AGTACT	1 cut(s)	438
Bme1390I	CCNGG	9 cut(s)	80, 428, 544, 685, 831, 1366, 1551, 2258, 2665
Bme18I	GGWCC	6 cut(s)	410, 673, 680, 1880, 2039, 2641
BmeRI	GACNNNNNGTC	1 cut(s)	203
BmeT110I	CYCGRG	1 cut(s)	2391
BmgT120I	GGNCC	8 cut(s)	410, 673, 680, 977, 1880, 2039, 2641, 3388
BmiI	GGNNCC	8 cut(s)	7, 412, 682, 1350, 1863, 2255, 2463, 2994
BmrFI	CCNGG	9 cut(s)	80, 428, 544, 685, 831, 1366, 1551, 2258, 2665
BmrI	ACTGGG	1 cut(s)	240
BmuI	ACTGGG	1 cut(s)	240
BpiI	GAAGAC	2 cut(s)	1070, 2994
BpmI	CTGGAG	1 cut(s)	2841
Bpu10I	CCTNAGC	1 cut(s)	2409
BpuEI	CTTGAG	5 cut(s)	824, 1778, 2028, 2305, 2803
BpuMI	CCSGG	5 cut(s)	80, 544, 1551, 2258, 2665
Bsa29I	ATCGAT	1 cut(s)	1251
BsaBI	GATNNNNATC	1 cut(s)	1251
BsaHI	GRCGYC	1 cut(s)	1595
BsaWI	WCCGGW	3 cut(s)	1705, 1768, 3222
BsaXI	ACNNNNNCTCC	6 cut(s)	205, 235, 1580, 1610, 3217, 3247
Bsc4I	CCNNNNNNNGG	5 cut(s)	366, 689, 2263, 3040, 3397
Bse8I	GATNNNNATC	1 cut(s)	1251
BseAI	TCCGGA	2 cut(s)	1768, 3222
BseBI	CCWGG	4 cut(s)	428, 685, 831, 1366
BseCI	ATCGAT	1 cut(s)	1251
BseJI	GATNNNNATC	1 cut(s)	1251
BseLI	CCNNNNNNNGG	5 cut(s)	366, 689, 2263, 3040, 3397
BseMII	CTCAG	5 cut(s)	234, 447, 2423, 2456, 3383
BseXI	GCAGC	7 cut(s)	511, 514, 847, 2101, 2266, 2546, 2672
BsgI	GTGCAG	5 cut(s)	978, 1812, 2136, 2265, 2679
BshFI	GGCC	7 cut(s)	979, 1369, 2064, 2829, 3184, 3251, 3389
BshVI	ATCGAT	1 cut(s)	1251
BsiHKAI	GWGCWC	1 cut(s)	3207
BsiHKCI	CYCGRG	1 cut(s)	2391
BsiSI	CCGG	8 cut(s)	79, 543, 1550, 1706, 1769, 2258, 2664, 3223
BslFI	GGGAC	5 cut(s)	396, 666, 1858, 2824, 3109
BslI	CCNNNNNNNGG	5 cut(s)	366, 689, 2263, 3040, 3397
BsmAI	GTCTC	4 cut(s)	152, 446, 1404, 1828
BsmFI	GGGAC	5 cut(s)	396, 666, 1858, 2824, 3109
BsmI	GAATGC	2 cut(s)	874, 2588
BsnI	GGCC	7 cut(s)	979, 1369, 2064, 2829, 3184, 3251, 3389
BsoBI	CYCGRG	1 cut(s)	2391
Bsp1286I	GDGCHC	1 cut(s)	3207
Bsp13I	TCCGGA	2 cut(s)	1768, 3222
Bsp19I	CCATGG	1 cut(s)	1242
BspACI	CCGC	5 cut(s)	420, 1390, 1538, 2908, 3074
BspANI	GGCC	7 cut(s)	979, 1369, 2064, 2829, 3184, 3251, 3389
BspCNI	CTCAG	5 cut(s)	235, 448, 2422, 2455, 3384
BspDI	ATCGAT	1 cut(s)	1251
BspEI	TCCGGA	2 cut(s)	1768, 3222
BspHI	TCATGA	3 cut(s)	756, 1144, 1357
BspLI	GGNNCC	8 cut(s)	7, 412, 682, 1350, 1863, 2255, 2463, 2994
BspMI	ACCTGC	2 cut(s)	1006, 3079
BspQI	GCTCTTC	1 cut(s)	3195
BspTI	CTTAAG	2 cut(s)	1985, 2285
BssNI	GRCGYC	1 cut(s)	1595
BssT1I	CCWWGG	6 cut(s)	9, 676, 1242, 1659, 2274, 3034
Bst2UI	CCWGG	4 cut(s)	428, 685, 831, 1366
Bst6I	CTCTTC	8 cut(s)	1197, 1377, 2172, 2220, 2410, 2487, 2837, 3195
BstACI	GRCGYC	1 cut(s)	1595
BstAFI	CTTAAG	2 cut(s)	1985, 2285
BstAPI	GCANNNNNTGC	2 cut(s)	1835, 2159
BstC8I	GCNNGC	6 cut(s)	970, 2151, 2602, 2906, 3076, 3253
BstDSI	CCRYGG	2 cut(s)	657, 1242
BstENI	CCTNNNNNAGG	1 cut(s)	3395
BstHHI	GCGC	1 cut(s)	1213
BstMAI	GTCTC	4 cut(s)	152, 446, 1404, 1828
BstMWI	GCNNNNNNNGC	6 cut(s)	1622, 1636, 1762, 1835, 2159, 3257
BstNI	CCWGG	4 cut(s)	428, 685, 831, 1366
BstNSI	RCATGY	2 cut(s)	1052, 2504
BstSCI	CCNGG	9 cut(s)	78, 426, 542, 683, 829, 1364, 1549, 2256, 2663
BstSFI	CTRYAG	2 cut(s)	2517, 2577
BstV1I	GCAGC	7 cut(s)	511, 514, 847, 2101, 2266, 2546, 2672
BstV2I	GAAGAC	2 cut(s)	1070, 2994
BstX2I	RGATCY	9 cut(s)	775, 1386, 1445, 1711, 1918, 2461, 2632, 2776, 2992
BstYI	RGATCY	9 cut(s)	775, 1386, 1445, 1711, 1918, 2461, 2632, 2776, 2992
Bsu15I	ATCGAT	1 cut(s)	1251
BsuI	GTATCC	1 cut(s)	2350
BsuRI	GGCC	7 cut(s)	979, 1369, 2064, 2829, 3184, 3251, 3389
BsuTUI	ATCGAT	1 cut(s)	1251
BtgI	CCRYGG	2 cut(s)	657, 1242
BtgZI	GCGATG	2 cut(s)	1361, 3270
BtsI	GCAGTG	1 cut(s)	1165
BtsIMutI	CAGTG	6 cut(s)	21, 279, 1098, 1165, 1933, 2683
BveI	ACCTGC	2 cut(s)	1006, 3079
Cac8I	GCNNGC	6 cut(s)	970, 2151, 2602, 2906, 3076, 3253
CaiI	CAGNNNCTG	2 cut(s)	266, 1262
CciI	TCATGA	3 cut(s)	756, 1144, 1357
CfoI	GCGC	1 cut(s)	1213
Cfr13I	GGNCC	8 cut(s)	410, 673, 680, 977, 1880, 2039, 2641, 3388
ClaI	ATCGAT	1 cut(s)	1251
CseI	GACGC	2 cut(s)	1584, 2606
CsiI	ACCWGGT	1 cut(s)	426
Csp6I	GTAC	6 cut(s)	437, 915, 1500, 2172, 2753, 2863
CviQI	GTAC	6 cut(s)	437, 915, 1500, 2172, 2753, 2863
DraI	TTTAAA	1 cut(s)	31
DrdI	GACNNNNNNGTC	1 cut(s)	605
DriI	GACNNNNNGTC	1 cut(s)	203
DseDI	GACNNNNNNGTC	1 cut(s)	605
EaeI	YGGCCR	1 cut(s)	2062
Eam1104I	CTCTTC	8 cut(s)	1197, 1377, 2172, 2220, 2410, 2487, 2837, 3195
Eam1105I	GACNNNNNGTC	1 cut(s)	203
EarI	CTCTTC	8 cut(s)	1197, 1377, 2172, 2220, 2410, 2487, 2837, 3195
EciI	GGCGGA	1 cut(s)	2923
Ecl136II	GAGCTC	1 cut(s)	3205
Eco130I	CCWWGG	6 cut(s)	9, 676, 1242, 1659, 2274, 3034
Eco147I	AGGCCT	1 cut(s)	3251
Eco24I	GRGCYC	1 cut(s)	3207
Eco32I	GATATC	1 cut(s)	1249
Eco47I	GGWCC	6 cut(s)	410, 673, 680, 1880, 2039, 2641
Eco53kI	GAGCTC	1 cut(s)	3205
Eco57I	CTGAAG	6 cut(s)	924, 1824, 2244, 2382, 2583, 3123
Eco88I	CYCGRG	1 cut(s)	2391
EcoICRI	GAGCTC	1 cut(s)	3205
EcoNI	CCTNNNNNAGG	1 cut(s)	3395
EcoRII	CCWGG	4 cut(s)	426, 683, 829, 1364
EcoRV	GATATC	1 cut(s)	1249
EcoT14I	CCWWGG	6 cut(s)	9, 676, 1242, 1659, 2274, 3034
EcoT22I	ATGCAT	1 cut(s)	3262
EcoT38I	GRGCYC	1 cut(s)	3207
ErhI	CCWWGG	6 cut(s)	9, 676, 1242, 1659, 2274, 3034
FalI	AAGNNNNNCTT	2 cut(s)	2931, 2963
FaqI	GGGAC	5 cut(s)	396, 666, 1858, 2824, 3109
FauI	CCCGC	2 cut(s)	427, 3067
FbaI	TGATCA	1 cut(s)	1555
Fnu4HI	GCNGC	7 cut(s)	525, 528, 836, 2115, 2280, 2535, 2661
FriOI	GRGCYC	1 cut(s)	3207
Fsp4HI	GCNGC	7 cut(s)	525, 528, 836, 2115, 2280, 2535, 2661
FspBI	CTAG	9 cut(s)	689, 873, 1214, 1314, 1626, 1716, 2118, 2474, 3006
GlaI	GCGC	1 cut(s)	1212
GluI	GCNGC	7 cut(s)	525, 528, 836, 2115, 2280, 2535, 2661
GsuI	CTGGAG	1 cut(s)	2841
HaeIII	GGCC	7 cut(s)	979, 1369, 2064, 2829, 3184, 3251, 3389
HapII	CCGG	8 cut(s)	79, 543, 1550, 1706, 1769, 2258, 2664, 3223
HgaI	GACGC	2 cut(s)	1584, 2606
HhaI	GCGC	1 cut(s)	1213
Hin1I	GRCGYC	1 cut(s)	1595
Hin6I	GCGC	1 cut(s)	1211
HinP1I	GCGC	1 cut(s)	1211
HincII	GTYRAC	1 cut(s)	462
HindII	GTYRAC	1 cut(s)	462
HindIII	AAGCTT	2 cut(s)	1935, 3377
HpaII	CCGG	8 cut(s)	79, 543, 1550, 1706, 1769, 2258, 2664, 3223
Hpy166II	GTNNAC	3 cut(s)	462, 654, 2753
Hpy8I	GTNNAC	3 cut(s)	462, 654, 2753
Hpy99I	CGWCG	1 cut(s)	320
HpyCH4IV	ACGT	1 cut(s)	640
HpyF10VI	GCNNNNNNNGC	6 cut(s)	1622, 1636, 1762, 1835, 2159, 3257
HpySE526I	ACGT	1 cut(s)	640
Hsp92I	GRCGYC	1 cut(s)	1595
HspAI	GCGC	1 cut(s)	1211
Kpn2I	TCCGGA	2 cut(s)	1768, 3222
Ksp22I	TGATCA	1 cut(s)	1555
LguI	GCTCTTC	1 cut(s)	3195
LmnI	GCTCC	6 cut(s)	13, 213, 598, 1348, 3036, 3404
Lsp1109I	GCAGC	7 cut(s)	511, 514, 847, 2101, 2266, 2546, 2672
MabI	ACCWGGT	1 cut(s)	426
MaeI	CTAG	9 cut(s)	689, 873, 1214, 1314, 1626, 1716, 2118, 2474, 3006
MaeII	ACGT	1 cut(s)	640
MfeI	CAATTG	1 cut(s)	1061
MflI	RGATCY	9 cut(s)	775, 1386, 1445, 1711, 1918, 2461, 2632, 2776, 2992
MhlI	GDGCHC	1 cut(s)	3207
MlyI	GAGTC	3 cut(s)	467, 1514, 2075
MmeI	TCCRAC	4 cut(s)	651, 1948, 2017, 3382
Mph1103I	ATGCAT	1 cut(s)	3262
MroI	TCCGGA	2 cut(s)	1768, 3222
MroXI	GAANNNNTTC	1 cut(s)	3140
MslI	CAYNNNNRTG	3 cut(s)	369, 2207, 2898
MspA1I	CMGCKG	1 cut(s)	422
MspCI	CTTAAG	2 cut(s)	1985, 2285
MspI	CCGG	8 cut(s)	79, 543, 1550, 1706, 1769, 2258, 2664, 3223
MspR9I	CCNGG	9 cut(s)	80, 428, 544, 685, 831, 1366, 1551, 2258, 2665
MunI	CAATTG	1 cut(s)	1061
Mva1269I	GAATGC	2 cut(s)	874, 2588
MvaI	CCWGG	4 cut(s)	428, 685, 831, 1366
MwoI	GCNNNNNNNGC	6 cut(s)	1622, 1636, 1762, 1835, 2159, 3257
NciI	CCSGG	5 cut(s)	80, 544, 1551, 2258, 2665
NcoI	CCATGG	1 cut(s)	1242
NlaIV	GGNNCC	8 cut(s)	7, 412, 682, 1350, 1863, 2255, 2463, 2994
NmeAIII	GCCGAG	2 cut(s)	2090, 3365
NmuCI	GTSAC	4 cut(s)	238, 374, 718, 1160
NsiI	ATGCAT	1 cut(s)	3262
NspI	RCATGY	2 cut(s)	1052, 2504
PagI	TCATGA	3 cut(s)	756, 1144, 1357
PaqCI	CACCTGC	1 cut(s)	1006
PasI	CCCWGGG	1 cut(s)	830
PceI	AGGCCT	1 cut(s)	3251
PciI	ACATGT	1 cut(s)	2500
PciSI	GCTCTTC	1 cut(s)	3195
PcsI	WCGNNNNNNNCGW	1 cut(s)	294
PctI	GAATGC	2 cut(s)	874, 2588
PdmI	GAANNNNTTC	1 cut(s)	3140
PflFI	GACNNNGTC	1 cut(s)	457
PflMI	CCANNNNNTGG	1 cut(s)	3040
PkrI	GCNGC	7 cut(s)	526, 529, 837, 2116, 2281, 2536, 2662
PleI	GAGTC	3 cut(s)	466, 1514, 2075
PpsI	GAGTC	3 cut(s)	466, 1514, 2075
PscI	ACATGT	1 cut(s)	2500
PshBI	ATTAAT	2 cut(s)	474, 947
Psp124BI	GAGCTC	1 cut(s)	3207
Psp6I	CCWGG	4 cut(s)	426, 683, 829, 1364
PspGI	CCWGG	4 cut(s)	426, 683, 829, 1364
PspN4I	GGNNCC	8 cut(s)	7, 412, 682, 1350, 1863, 2255, 2463, 2994
PspPI	GGNCC	8 cut(s)	410, 673, 680, 977, 1880, 2039, 2641, 3388
PstNI	CAGNNNCTG	2 cut(s)	266, 1262
PsuI	RGATCY	9 cut(s)	775, 1386, 1445, 1711, 1918, 2461, 2632, 2776, 2992
PsyI	GACNNNGTC	1 cut(s)	457
RsaI	GTAC	6 cut(s)	438, 916, 1501, 2173, 2754, 2864
RsaNI	GTAC	6 cut(s)	437, 915, 1500, 2172, 2753, 2863
RseI	CAYNNNNRTG	3 cut(s)	369, 2207, 2898
SacI	GAGCTC	1 cut(s)	3207
SapI	GCTCTTC	1 cut(s)	3195
SatI	GCNGC	7 cut(s)	525, 528, 836, 2115, 2280, 2535, 2661
Sau96I	GGNCC	8 cut(s)	410, 673, 680, 977, 1880, 2039, 2641, 3388
ScaI	AGTACT	1 cut(s)	438
SchI	GAGTC	3 cut(s)	467, 1514, 2075
ScrFI	CCNGG	9 cut(s)	80, 428, 544, 685, 831, 1366, 1551, 2258, 2665
SduI	GDGCHC	1 cut(s)	3207
SexAI	ACCWGGT	1 cut(s)	426
SfcI	CTRYAG	2 cut(s)	2517, 2577
SinI	GGWCC	6 cut(s)	410, 673, 680, 1880, 2039, 2641
SmiMI	CAYNNNNRTG	3 cut(s)	369, 2207, 2898
SmlI	CTYRAG	7 cut(s)	803, 1757, 1985, 2043, 2285, 2320, 2782
SmoI	CTYRAG	7 cut(s)	803, 1757, 1985, 2043, 2285, 2320, 2782
SseBI	AGGCCT	1 cut(s)	3251
SsiI	CCGC	5 cut(s)	420, 1390, 1538, 2908, 3074
SspI	AATATT	1 cut(s)	67
SspMI	CTAG	9 cut(s)	689, 873, 1214, 1314, 1626, 1716, 2118, 2474, 3006
SstI	GAGCTC	1 cut(s)	3207
StuI	AGGCCT	1 cut(s)	3251
StyD4I	CCNGG	9 cut(s)	78, 426, 542, 683, 829, 1364, 1549, 2256, 2663
StyI	CCWWGG	6 cut(s)	9, 676, 1242, 1659, 2274, 3034
TaiI	ACGT	1 cut(s)	643
TaqI	TCGA	5 cut(s)	433, 496, 1251, 2770, 3278
TatI	WGTACW	2 cut(s)	436, 2171
TscAI	CASTG	6 cut(s)	28, 286, 1098, 1165, 1933, 2683
TseFI	GTSAC	4 cut(s)	238, 374, 718, 1160
TseI	GCWGC	7 cut(s)	524, 527, 835, 2114, 2279, 2534, 2660
Tsp45I	GTSAC	4 cut(s)	238, 374, 718, 1160
TspGWI	ACGGA	4 cut(s)	461, 1101, 1438, 1893
TspRI	CASTG	6 cut(s)	28, 286, 1098, 1165, 1933, 2683
Tth111I	GACNNNGTC	1 cut(s)	457
Van91I	CCANNNNNTGG	1 cut(s)	3040
Vha464I	CTTAAG	2 cut(s)	1985, 2285
VpaK11BI	GGWCC	6 cut(s)	410, 673, 680, 1880, 2039, 2641
VspI	ATTAAT	2 cut(s)	474, 947
XagI	CCTNNNNNAGG	1 cut(s)	3395
XbaI	TCTAGA	1 cut(s)	2473
XceI	RCATGY	2 cut(s)	1052, 2504
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2635
XmnI	GAANNNNTTC	1 cut(s)	3140
XspI	CTAG	9 cut(s)	689, 873, 1214, 1314, 1626, 1716, 2118, 2474, 3006
ZrmI	AGTACT	1 cut(s)	438
Zsp2I	ATGCAT	1 cut(s)	3262

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.