Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr6

Orthogroup ID

OG0005883

Physical Location & Seq

Genomic Coordinates Reverse (-)

39462312 .. 39469425

Gene Structure 7114 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw6G019770.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3396 bp

ATGGGAAGCAAGCAGCATGACAGTGTTTTTAAAGACGATGAACCTACTACTGTGGACCATGATGATAGTTCTTTGATCCAGGTTGAGTTTTCAACTGATTCAAATGTGAATAATGAATTTAAAAAGTTGAGTGAGGTTGTCGGTGGCATGAATAATGCTCCATACATATATAGACAAGATGTTGTGAGGAGCAAGAATGGTATGATTGGGGTTGTGACTGAAGTTGCTGGTGATTTGGATTCTGATAGTGGCATGTCTGATGATGAGGATGAGGATGAGGATGAAATAGATGACGACGATGACGACGACCACAACAGTGAGAATCATGAAAATGACAAAGATGGTGGTGATGACAGTAATACTAATGAAAATGGTGACAAGAACCAGAATGGTGGTAATGATAAGACTGGTCCTCTTCCTGCAGACCAGGTTCGAGTGCTTTGGATAGATGAAACTGAGTCAACCCAGAATATCAATGATATAACAGTTGTTGATCGAGGATTCTTGCATGGAGATTTTGTTGCTGCTGCTTCTGAGCCAACCGGGCAAGTTGGTGTCGTGGTGGATGTCAATATCTCTGTTGATCTGAAAGCTCCTGACGGGTCCATCGTAAAAGATGTGTCAGCCAATGACTTAAAACGTGTGAGGGAGTTCACTGTTGGTGATTATGTGGTCCTTGGTACCTGGCTGGGTAGAATTGAGGATGTTTTTGATAATGTGACGGTGATGATTGATGATGGTTCCCTATGTAGAATTTTGAGAGCAGATCCAATGGATCTTAAACCACTTTCCAAAAATATGCTTGAAGATGTGCATTTCCCTTATTACCCAGGCCAGCGTGTGAAGGCAAGGTCATCATCGGTTTTTAAGAATTCTTTGTGGCTATCCGGCTCATGGAAGGCTAATCGTCTGGAAGGTACTGTTACTAAAGTTACAGTGGGATCAGTGTTTATTTATTGGATAGCTTCTGCTGGCTGTGGCCCAGATTCTTCAACTGCCCCTGCTAAAGAGCAGGTCCCCAGGAAGTTGAAACTATTGTCTTGTTTCACACATGCAAATTGGCAGTTGGGCGACTGGTGTCTCTTTCCATCATCAGGGTCATCATCTTCCACTGCCGTAGATGAGGGTTCAAAATTAGAACTTCATCAATTAGATTCTACTCAAATGGGTAATGTGTGTGATTCGGAAGAGAGTTCACTAGAAGAACCAAATGGAAATAGTGCATCCATGGATACTGACCCGGTGTCTGTATTAGATGGAAACAATGGAAATGCTGGAAGTAATACTTCTATTGAATCTAGTTCCAGCGGTAGTTCATTGTCAGTCACAAAGGTACCTGTTTCTCTTCATCGCAAAAAGCTGCGTAAACCTGTGGTTAAAAGAGACAAGAAGGCACGAAAGGAGGAAAATTTTGAAAGATCTTTTTTGATTGCCAATACCAGGACAACAGTTGATGTGGCATGGCAGGATGGTTCAACAGAACGGAAGCTGGCTTCAACGAATTTAATTCCTCTTGATAGTCCTGGTGATCATGAATTTGTTGCTGAACAATATGTGGTGGAGAAGGCATCTGATGATGGTGACAATGCTTTTGAAGCTAGGCGTGTTGGACTCGTGAAAAGCGTCAATGCCAAGGAGCGAACAGCTTGTGTACAGTGGTTGAAGGCAGTTTCCAGAGCTGAAGATCCTAGAGAGTTTGACAAGGAGGAAGTTGTTAGTGTGTATGAGCTTGAGGGTCACCCAGATTATGATTACTGTTATGGGGATGTTGTTGTCAGATTGTTGCCAGTTTCTGTTTCTGCACAAACTGCTTCGGTATCGGACTTAATTGAGGAGCCAAAGCAGCAAAATCAACCAAGTGAGCTTACATCAGAAGTTGTTGATGAAGCTTCTTCTGTTGATAAATCTTGTATGGACTTCTCAGATCTTTCTTGGGTTGGGAATATAACTGGTCTTAAGAATGGTGATATTGAAGTTACATGGGCTGACGGGATGGTTTCTACGGTTGGACCTCAAGCAATTTATGTTGTTGGTCGAGCTGATGATGATGACTCAATTGCTGGGAGCGAGGTTAGTGATGCTGCTAGTTGGGAAACAGTTGATGATGAGGAGGTGCATGCCCTATTTCCCCTCGAGAGTATAACAGAGGAAGTGGAGATACAGAGTGCCTTTAACATGAATTCTGAAGCAGAGGTGGGTGGAGAAGATAATTCTGGAAGGAACCCTGCATTGTCTGTTCCCTTGGCTGCATTTCGCTTTGTTACCAGACTGGCAAGTGGAATATTTGCTCGGGGACAAAAGAACTTGGATCCAATTTTAGATACTAAAGGTGAAGGTGATTTTGAGCCTCGGGAAGTTGAAATCTTTCAGGGAGATCATTGTGAAGATTCCAGCTCTCAGAAATCTAAAGTTGTTGATATTAGTGTGGAGACCAATCATAAAAATGAAGAGCATGTTTCCTCGGAGACAACAAAAATATTGGATGCAGCAGAAATCTTGTGCAACTTAAGGACTGAAGGATTGGATGCTAAAGAATGTAGCAAGGGTGATGTTTGCAGTTTCAAACGCTTTGATATAGTCAAAGATCCTTTGGACCATCATTATCTTGGTGCAGCAGGGCAGGATAGTGGTGGTAAGAAGTGGTTCAAGAAGATTCAGCAAGATTGGGGTATCCTTCAAAATAACCTGCCTGATGGCATCTACGTACGCGTTTATGAAGATCGAATGGATCTCTTGAGGGCAGTTATAGTTGGGGCTTATGGGACACCTTACCAAGATGGCCTCTTCTTTTTCGATTTTCATCTCCCTCCGGAGTACCCTGATGTCCCTCCGTCAGCATATTATCATTCTGGTGGCTGGCGGATAAATCCAAATCTATATGAAGAAGGCAAAGTCTGTCTTAGTCTGTTAAATACTTGGACTGGTAGAGGAAATGAAGTTTGGGATCCAAAGTCTTCTAGCATCCTTCAAGTTCTAGTTTCACTCCAGGGATTGGTGCTAAATTCTAAGCCCTATTTCAACGAAGCTGGCTATGATAAGCAAATTGGAACAGCTGAAGGGGAGAAAAATTCGTTGTCCTACAATGAGAATACTTTCTTGCTGAATTGCAAGACGATAATGTCTCTTATGCGGAGGCCTCCCAAGGACTTTGAAGAGCTTGTTCAAGACCATTTCAGGAGGCGTGGTTATTATATCCTCAAGGCTTGCGATGCATATATGAAAGGCTACTTGATAGGTTCGCTCACTAAAGATGCCTCTGTTAGTGATAGCAGTGATGCAAATTCAACCTCTGTTGGTTTCAAGTTGATGTTAGCTAAGATTGTGCCAAAGCTGTTCTTGGCTTTGAGTGAGGTTGGAGCCGATTGTCATGAATTTAAGCATCTGCAACAGTCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006464 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019899 GO:0031625 GO:0032446 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044389 GO:0044424 GO:0044444 GO:0044464 GO:0061630 GO:0061659 GO:0070647 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04120 map04120

Pfam Domains

Protein Families

UQ_con

Protein Analysis

1131

Amino Acids

124.47

Weight (kDa)

4.54

Isoelectric Point (pI)

37.09

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SH3-A_UBE2O	PF23048	56 - 164	7.2e-06	UBE2O, N-terminal SH3-A domain
tSH3-B_UBE2O	PF23046	220 - 351	1.7e-42	UBE2O, tandem tSH3-B domain
SH3-B_UBE2O	PF23043	510 - 582	3.6e-20	UBE2O, SH3-B domain
SH3-C_UBE2O	PF23044	641 - 683	1.6e-06	UBE2O, ubiquitin binding SH3-C domain
UQ_con	PF00179	885 - 1017	8.4e-20	Ubiquitin-conjugating enzyme

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0005883)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G16920 AT2G16920
fragaria_vesca	FvH4_2g15490 FvH4_2g15490 FvH4_2g15490 FvH4_2g15490 FvH4_2g15490 FvH4_2g15490 FvH4_2g15490
malus_domestica	MD05G1123200.v1.1 MD10G1126200.v1.1
prunus_persica	Prupe.8G167100_v2.0.a1 Prupe.8G167100_v2.0.a1 Prupe.8G167100_v2.0.a1
pyrus_communis	pycom05g11760 pycom10g10840
rosa_chinensis	RchiOBHm_Chr6g0278491
rosa_laevigata	RLG00000013224
rosa_multiflora	Rmu_sc0000258.1_g000006
rosa_roxburghii	Rroxscaffold_7G00189850 Rroxscaffold_7G00190340
rosa_rugosa	Rorug06G0117200 Rorug06G0117200
rosa_samantha	Rh6AG226500 Rh6BG230700 Rh6CG233800 Rh6DG225100
rosa_wichuraiana	Rw6G019770

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	3118
Acc36I	ACCTGC	2 cut(s)	1003, 2694
Acc65I	GGTACC	2 cut(s)	680, 1333
AccB1I	GGYRCC	2 cut(s)	680, 1333
AccB7I	CCANNNNNTGG	1 cut(s)	2992
AccII	CGCG	1 cut(s)	2709
AccIII	TCCGGA	1 cut(s)	2809
AciI	CCGC	3 cut(s)	1308, 2860, 3130
AcuI	CTGAAG	5 cut(s)	240, 1701, 2205, 2535, 3075
AfaI	GTAC	6 cut(s)	682, 919, 1335, 1653, 2706, 2816
AfiI	CCNNNNNNNGG	4 cut(s)	894, 1094, 1440, 2992
AflII	CTTAAG	2 cut(s)	1955, 2506
AflIII	ACRYGT	2 cut(s)	640, 2707
AhdI	GACNNNNNGTC	1 cut(s)	1243
AjnI	CCWGG	8 cut(s)	78, 426, 683, 829, 1019, 1439, 1522, 2985
AjuI	GAANNNNNNNTTGG	2 cut(s)	2923, 2955
AleI	CACNNNNGTG	1 cut(s)	315
Alw26I	GTCTC	5 cut(s)	1085, 1377, 2423, 2459, 3126
AlwNI	CAGNNNCTG	1 cut(s)	1793
Ama87I	CYCGRG	3 cut(s)	2132, 2289, 2349
Aor13HI	TCCGGA	1 cut(s)	2809
AoxI	GGCC	4 cut(s)	832, 979, 2779, 3134
ApeKI	GCWGC	9 cut(s)	13, 524, 527, 1360, 1843, 2081, 2246, 2486, 2612
ArsI	GACNNNNNNTTYG	2 cut(s)	1574, 1606
Asp700I	GAANNNNTTC	3 cut(s)	2364, 2642, 3092
Asp718I	GGTACC	2 cut(s)	680, 1333
AspS9I	GGNCC	8 cut(s)	55, 410, 603, 673, 980, 1015, 2009, 2593
AsuC2I	CCSGG	2 cut(s)	544, 1241
AvaI	CYCGRG	3 cut(s)	2132, 2289, 2349
AvaII	GGWCC	7 cut(s)	55, 410, 603, 673, 1015, 2009, 2593
BaeI	ACNNNNGTAYC	2 cut(s)	1317, 1350
BamHI	GGATCC	2 cut(s)	2308, 2944
BanI	GGYRCC	2 cut(s)	680, 1333
BarI	GAAGNNNNNNTAC	2 cut(s)	2142, 2174
BauI	CACGAG	1 cut(s)	1613
BbsI	GAAGAC	1 cut(s)	2946
BbvI	GCAGC	9 cut(s)	25, 511, 514, 1347, 1855, 2068, 2233, 2498, 2624
BceAI	ACGGC	1 cut(s)	1100
BciT130I	CCWGG	8 cut(s)	80, 428, 685, 831, 1021, 1441, 1524, 2987
BciVI	GTATCC	2 cut(s)	1225, 2681
BclI	TGATCA	1 cut(s)	1528
BcnI	CCSGG	2 cut(s)	544, 1241
BcoDI	GTCTC	5 cut(s)	1085, 1377, 2423, 2459, 3126
BfaI	CTAG	7 cut(s)	1199, 1299, 1599, 1689, 2085, 2958, 2975
BfmI	CTRYAG	1 cut(s)	420
BfrI	CTTAAG	2 cut(s)	1955, 2506
BfuAI	ACCTGC	2 cut(s)	1003, 2694
BfuI	GTATCC	2 cut(s)	1225, 2681
BglI	GCCNNNNNGGC	1 cut(s)	544
BglII	AGATCT	2 cut(s)	1418, 1924
BisI	GCNGC	9 cut(s)	14, 525, 528, 1361, 1844, 2082, 2247, 2487, 2613
BlsI	GCNGC	9 cut(s)	15, 526, 529, 1362, 1845, 2083, 2248, 2488, 2614
Bme18I	GGWCC	7 cut(s)	55, 410, 603, 673, 1015, 2009, 2593
BmeRI	GACNNNNNGTC	1 cut(s)	1243
BmeT110I	CYCGRG	3 cut(s)	2132, 2289, 2349
BmgT120I	GGNCC	8 cut(s)	55, 410, 603, 673, 980, 1015, 2009, 2593
BoxI	GACNNNNGTC	1 cut(s)	1077
BpiI	GAAGAC	1 cut(s)	2946
BpmI	CTGGAG	1 cut(s)	2969
BpuEI	CTTGAG	4 cut(s)	1751, 1998, 2755, 3182
BpuMI	CCSGG	2 cut(s)	544, 1241
BsaAI	YACGTR	1 cut(s)	2704
BsaBI	GATNNNNATC	1 cut(s)	2799
BsaI	GGTCTC	1 cut(s)	2423
BsaWI	WCCGGW	1 cut(s)	2809
BsaXI	ACNNNNNCTCC	4 cut(s)	504, 534, 3169, 3199
Bsc4I	CCNNNNNNNGG	4 cut(s)	894, 1094, 1440, 2992
Bse1I	ACTGG	6 cut(s)	412, 1079, 1787, 1954, 2274, 2926
Bse8I	GATNNNNATC	1 cut(s)	2799
BseAI	TCCGGA	1 cut(s)	2809
BseBI	CCWGG	8 cut(s)	80, 428, 685, 831, 1021, 1441, 1524, 2987
BseJI	GATNNNNATC	1 cut(s)	2799
BseLI	CCNNNNNNNGG	4 cut(s)	894, 1094, 1440, 2992
BseMII	CTCAG	4 cut(s)	447, 525, 1935, 2411
BseNI	ACTGG	6 cut(s)	412, 1079, 1787, 1954, 2274, 2926
BseRI	GAGGAG	3 cut(s)	202, 1847, 2123
BseXI	GCAGC	9 cut(s)	25, 511, 514, 1347, 1855, 2068, 2233, 2498, 2624
BseYI	CCCAGC	2 cut(s)	688, 2060
BsgI	GTGCAG	2 cut(s)	1785, 2631
Bsh1236I	CGCG	1 cut(s)	2709
BshFI	GGCC	4 cut(s)	834, 981, 2781, 3136
BshNI	GGYRCC	2 cut(s)	680, 1333
BsiHKCI	CYCGRG	3 cut(s)	2132, 2289, 2349
BsiSI	CCGG	4 cut(s)	543, 888, 1241, 2810
BsiWI	CGTACG	1 cut(s)	2704
BslFI	GGGAC	4 cut(s)	1001, 2307, 2776, 2810
BslI	CCNNNNNNNGG	4 cut(s)	894, 1094, 1440, 2992
BsmAI	GTCTC	5 cut(s)	1085, 1377, 2423, 2459, 3126
BsmFI	GGGAC	4 cut(s)	1001, 2307, 2776, 2810
BsnI	GGCC	4 cut(s)	834, 981, 2781, 3136
Bso31I	GGTCTC	1 cut(s)	2423
BsoBI	CYCGRG	3 cut(s)	2132, 2289, 2349
Bsp13I	TCCGGA	1 cut(s)	2809
Bsp1407I	TGTACA	1 cut(s)	1651
Bsp19I	CCATGG	1 cut(s)	1227
BspACI	CCGC	3 cut(s)	1308, 2860, 3130
BspANI	GGCC	4 cut(s)	834, 981, 2781, 3136
BspCNI	CTCAG	4 cut(s)	448, 526, 1934, 2410
BspEI	TCCGGA	1 cut(s)	2809
BspFNI	CGCG	1 cut(s)	2709
BspHI	TCATGA	3 cut(s)	325, 1531, 3367
BspMAI	CTGCAG	1 cut(s)	424
BspMI	ACCTGC	2 cut(s)	1003, 2694
BspQI	GCTCTTC	2 cut(s)	2442, 3147
BspT107I	GGYRCC	2 cut(s)	680, 1333
BspTI	CTTAAG	2 cut(s)	1955, 2506
BspTNI	GGTCTC	1 cut(s)	2423
BsrGI	TGTACA	1 cut(s)	1651
BsrI	ACTGG	6 cut(s)	412, 1079, 1787, 1954, 2274, 2926
BssSI	CACGAG	1 cut(s)	1613
BssT1I	CCWWGG	5 cut(s)	676, 1227, 1632, 2241, 3141
Bst2BI	CACGAG	1 cut(s)	1613
Bst2UI	CCWGG	8 cut(s)	80, 428, 685, 831, 1021, 1441, 1524, 2987
Bst6I	CTCTTC	6 cut(s)	420, 1182, 1350, 2442, 2789, 3147
BstAFI	CTTAAG	2 cut(s)	1955, 2506
BstAPI	GCANNNNNTGC	1 cut(s)	1808
BstAUI	TGTACA	1 cut(s)	1651
BstBAI	YACGTR	1 cut(s)	2704
BstC8I	GCNNGC	8 cut(s)	11, 836, 973, 1491, 2118, 2858, 3028, 3205
BstDEI	CTNAG	7 cut(s)	456, 534, 1921, 2397, 2900, 3006, 3315
BstDSI	CCRYGG	1 cut(s)	1227
BstEII	GGTNACC	1 cut(s)	1736
BstFNI	CGCG	1 cut(s)	2709
BstMAI	GTCTC	5 cut(s)	1085, 1377, 2423, 2459, 3126
BstMWI	GCNNNNNNNGC	5 cut(s)	544, 1595, 1808, 1843, 3209
BstNI	CCWGG	8 cut(s)	80, 428, 685, 831, 1021, 1441, 1524, 2987
BstNSI	RCATGY	4 cut(s)	256, 1055, 2120, 2456
BstPAI	GACNNNNGTC	1 cut(s)	1077
BstPI	GGTNACC	1 cut(s)	1736
BstSFI	CTRYAG	1 cut(s)	420
BstSNI	TACGTA	1 cut(s)	2704
BstUI	CGCG	1 cut(s)	2709
BstV1I	GCAGC	9 cut(s)	25, 511, 514, 1347, 1855, 2068, 2233, 2498, 2624
BstV2I	GAAGAC	1 cut(s)	2946
BstX2I	RGATCY	9 cut(s)	766, 775, 1418, 1684, 1924, 2308, 2584, 2728, 2944
BstXI	CCANNNNNNTGG	1 cut(s)	392
BstYI	RGATCY	9 cut(s)	766, 775, 1418, 1684, 1924, 2308, 2584, 2728, 2944
BsuI	GTATCC	2 cut(s)	1225, 2681
BsuRI	GGCC	4 cut(s)	834, 981, 2781, 3136
BtgI	CCRYGG	1 cut(s)	1227
BtgZI	GCGATG	2 cut(s)	1334, 3222
BtsI	GCAGTG	2 cut(s)	1110, 3277
BtsIMutI	CAGTG	8 cut(s)	28, 322, 654, 942, 951, 1110, 1661, 3277
BveI	ACCTGC	2 cut(s)	1003, 2694
Cac8I	GCNNGC	8 cut(s)	11, 836, 973, 1491, 2118, 2858, 3028, 3205
CaiI	CAGNNNCTG	1 cut(s)	1793
CciI	TCATGA	3 cut(s)	325, 1531, 3367
Cfr13I	GGNCC	8 cut(s)	55, 410, 603, 673, 980, 1015, 2009, 2593
CseI	GACGC	1 cut(s)	1612
CsiI	ACCWGGT	1 cut(s)	426
Csp6I	GTAC	6 cut(s)	681, 918, 1334, 1652, 2705, 2815
CspCI	CAANNNNNGTGG	2 cut(s)	325, 360
CviQI	GTAC	6 cut(s)	681, 918, 1334, 1652, 2705, 2815
DdeI	CTNAG	7 cut(s)	456, 534, 1921, 2397, 2900, 3006, 3315
DraI	TTTAAA	2 cut(s)	31, 121
DrdI	GACNNNNNNGTC	1 cut(s)	3118
DriI	GACNNNNNGTC	1 cut(s)	1243
DseDI	GACNNNNNNGTC	1 cut(s)	3118
Eam1104I	CTCTTC	6 cut(s)	420, 1182, 1350, 2442, 2789, 3147
Eam1105I	GACNNNNNGTC	1 cut(s)	1243
EarI	CTCTTC	6 cut(s)	420, 1182, 1350, 2442, 2789, 3147
EciI	GGCGGA	1 cut(s)	2875
Eco105I	TACGTA	1 cut(s)	2704
Eco130I	CCWWGG	5 cut(s)	676, 1227, 1632, 2241, 3141
Eco147I	AGGCCT	1 cut(s)	3136
Eco31I	GGTCTC	1 cut(s)	2423
Eco47I	GGWCC	7 cut(s)	55, 410, 603, 673, 1015, 2009, 2593
Eco57I	CTGAAG	5 cut(s)	240, 1701, 2205, 2535, 3075
Eco88I	CYCGRG	3 cut(s)	2132, 2289, 2349
Eco91I	GGTNACC	1 cut(s)	1736
EcoO109I	RGGNCCY	1 cut(s)	1015
EcoO65I	GGTNACC	1 cut(s)	1736
EcoRI	GAATTC	2 cut(s)	871, 2179
EcoRII	CCWGG	8 cut(s)	78, 426, 683, 829, 1019, 1439, 1522, 2985
EcoT14I	CCWWGG	5 cut(s)	676, 1227, 1632, 2241, 3141
EcoT22I	ATGCAT	1 cut(s)	3214
ErhI	CCWWGG	5 cut(s)	676, 1227, 1632, 2241, 3141
FalI	AAGNNNNNCTT	6 cut(s)	1270, 1302, 2883, 2915, 3320, 3352
FaqI	GGGAC	4 cut(s)	1001, 2307, 2776, 2810
FbaI	TGATCA	1 cut(s)	1528
Fnu4HI	GCNGC	9 cut(s)	14, 525, 528, 1361, 1844, 2082, 2247, 2487, 2613
Fsp4HI	GCNGC	9 cut(s)	14, 525, 528, 1361, 1844, 2082, 2247, 2487, 2613
FspBI	CTAG	7 cut(s)	1199, 1299, 1599, 1689, 2085, 2958, 2975
GluI	GCNGC	9 cut(s)	14, 525, 528, 1361, 1844, 2082, 2247, 2487, 2613
GsaI	CCCAGC	2 cut(s)	692, 2064
GsuI	CTGGAG	1 cut(s)	2969
HaeIII	GGCC	4 cut(s)	834, 981, 2781, 3136
HapII	CCGG	4 cut(s)	543, 888, 1241, 2810
HgaI	GACGC	1 cut(s)	1612
HincII	GTYRAC	1 cut(s)	462
HindII	GTYRAC	1 cut(s)	462
HindIII	AAGCTT	1 cut(s)	1887
HpaII	CCGG	4 cut(s)	543, 888, 1241, 2810
Hpy166II	GTNNAC	6 cut(s)	55, 462, 654, 1196, 1367, 1652
Hpy8I	GTNNAC	6 cut(s)	55, 462, 654, 1196, 1367, 1652
Hpy99I	CGWCG	2 cut(s)	299, 308
HpyCH4IV	ACGT	2 cut(s)	640, 2703
HpyF10VI	GCNNNNNNNGC	5 cut(s)	544, 1595, 1808, 1843, 3209
HpyF3I	CTNAG	7 cut(s)	456, 534, 1921, 2397, 2900, 3006, 3315
HpySE526I	ACGT	2 cut(s)	640, 2703
Kpn2I	TCCGGA	1 cut(s)	2809
KpnI	GGTACC	2 cut(s)	684, 1337
Ksp22I	TGATCA	1 cut(s)	1528
LguI	GCTCTTC	2 cut(s)	2442, 3147
LmnI	GCTCC	7 cut(s)	163, 189, 598, 1636, 1834, 2064, 3356
Lsp1109I	GCAGC	9 cut(s)	25, 511, 514, 1347, 1855, 2068, 2233, 2498, 2624
MabI	ACCWGGT	1 cut(s)	426
MaeI	CTAG	7 cut(s)	1199, 1299, 1599, 1689, 2085, 2958, 2975
MaeII	ACGT	2 cut(s)	640, 2703
MfeI	CAATTG	1 cut(s)	2055
MflI	RGATCY	9 cut(s)	766, 775, 1418, 1684, 1924, 2308, 2584, 2728, 2944
MluI	ACGCGT	1 cut(s)	2707
MlyI	GAGTC	3 cut(s)	467, 1605, 2045
MmeI	TCCRAC	3 cut(s)	1588, 1987, 3334
Mph1103I	ATGCAT	1 cut(s)	3214
MroI	TCCGGA	1 cut(s)	2809
MroXI	GAANNNNTTC	3 cut(s)	2364, 2642, 3092
MslI	CAYNNNNRTG	5 cut(s)	21, 315, 330, 2442, 2850
MspA1I	CMGCKG	2 cut(s)	1308, 3053
MspCI	CTTAAG	2 cut(s)	1955, 2506
MspI	CCGG	4 cut(s)	543, 888, 1241, 2810
MunI	CAATTG	1 cut(s)	2055
MvaI	CCWGG	8 cut(s)	80, 428, 685, 831, 1021, 1441, 1524, 2987
MvnI	CGCG	1 cut(s)	2709
MwoI	GCNNNNNNNGC	5 cut(s)	544, 1595, 1808, 1843, 3209
NciI	CCSGG	2 cut(s)	544, 1241
NcoI	CCATGG	1 cut(s)	1227
NmuCI	GTSAC	6 cut(s)	214, 374, 718, 1324, 1580, 1736
NsiI	ATGCAT	1 cut(s)	3214
NspI	RCATGY	4 cut(s)	256, 1055, 2120, 2456
OliI	CACNNNNGTG	1 cut(s)	315
PaeI	GCATGC	1 cut(s)	2120
PaeR7I	CTCGAG	1 cut(s)	2132
PagI	TCATGA	3 cut(s)	325, 1531, 3367
PceI	AGGCCT	1 cut(s)	3136
PciSI	GCTCTTC	2 cut(s)	2442, 3147
PcsI	WCGNNNNNNNCGW	4 cut(s)	300, 303, 606, 1620
PdmI	GAANNNNTTC	3 cut(s)	2364, 2642, 3092
Pfl23II	CGTACG	1 cut(s)	2704
PflMI	CCANNNNNTGG	1 cut(s)	2992
PkrI	GCNGC	9 cut(s)	15, 526, 529, 1362, 1845, 2083, 2248, 2488, 2614
PleI	GAGTC	3 cut(s)	466, 1605, 2045
PpsI	GAGTC	3 cut(s)	466, 1605, 2045
Ppu21I	YACGTR	1 cut(s)	2704
PpuMI	RGGWCCY	1 cut(s)	1015
PshAI	GACNNNNGTC	1 cut(s)	1077
Psp5II	RGGWCCY	1 cut(s)	1015
Psp6I	CCWGG	8 cut(s)	78, 426, 683, 829, 1019, 1439, 1522, 2985
PspEI	GGTNACC	1 cut(s)	1736
PspFI	CCCAGC	2 cut(s)	688, 2060
PspGI	CCWGG	8 cut(s)	78, 426, 683, 829, 1019, 1439, 1522, 2985
PspLI	CGTACG	1 cut(s)	2704
PspPI	GGNCC	8 cut(s)	55, 410, 603, 673, 980, 1015, 2009, 2593
PspPPI	RGGWCCY	1 cut(s)	1015
PstI	CTGCAG	1 cut(s)	424
PstNI	CAGNNNCTG	1 cut(s)	1793
PsuI	RGATCY	9 cut(s)	766, 775, 1418, 1684, 1924, 2308, 2584, 2728, 2944
PvuII	CAGCTG	1 cut(s)	3053
RsaI	GTAC	6 cut(s)	682, 919, 1335, 1653, 2706, 2816
RsaNI	GTAC	6 cut(s)	681, 918, 1334, 1652, 2705, 2815
RseI	CAYNNNNRTG	5 cut(s)	21, 315, 330, 2442, 2850
SapI	GCTCTTC	2 cut(s)	2442, 3147
SatI	GCNGC	9 cut(s)	14, 525, 528, 1361, 1844, 2082, 2247, 2487, 2613
Sau96I	GGNCC	8 cut(s)	55, 410, 603, 673, 980, 1015, 2009, 2593
SchI	GAGTC	3 cut(s)	467, 1605, 2045
SexAI	ACCWGGT	1 cut(s)	426
SfcI	CTRYAG	1 cut(s)	420
Sfr274I	CTCGAG	1 cut(s)	2132
SinI	GGWCC	7 cut(s)	55, 410, 603, 673, 1015, 2009, 2593
SlaI	CTCGAG	1 cut(s)	2132
SmiMI	CAYNNNNRTG	5 cut(s)	21, 315, 330, 2442, 2850
SmlI	CTYRAG	7 cut(s)	1730, 1955, 2013, 2132, 2506, 2734, 3197
SmoI	CTYRAG	7 cut(s)	1730, 1955, 2013, 2132, 2506, 2734, 3197
SnaBI	TACGTA	1 cut(s)	2704
SphI	GCATGC	1 cut(s)	2120
SseBI	AGGCCT	1 cut(s)	3136
SsiI	CCGC	3 cut(s)	1308, 2860, 3130
SspI	AATATT	2 cut(s)	2283, 2478
SspMI	CTAG	7 cut(s)	1199, 1299, 1599, 1689, 2085, 2958, 2975
StuI	AGGCCT	1 cut(s)	3136
StyI	CCWWGG	5 cut(s)	676, 1227, 1632, 2241, 3141
TaiI	ACGT	2 cut(s)	643, 2706
TaqI	TCGA	6 cut(s)	433, 496, 2035, 2133, 2722, 2793
TatI	WGTACW	1 cut(s)	1651
TscAI	CASTG	8 cut(s)	28, 322, 661, 942, 951, 1117, 1661, 3277
TseFI	GTSAC	6 cut(s)	214, 374, 718, 1324, 1580, 1736
TseI	GCWGC	9 cut(s)	13, 524, 527, 1360, 1843, 2081, 2246, 2486, 2612
Tsp45I	GTSAC	6 cut(s)	214, 374, 718, 1324, 1580, 1736
TspGWI	ACGGA	2 cut(s)	1498, 2820
TspRI	CASTG	8 cut(s)	28, 322, 661, 942, 951, 1117, 1661, 3277
Van91I	CCANNNNNTGG	1 cut(s)	2992
Vha464I	CTTAAG	2 cut(s)	1955, 2506
VpaK11BI	GGWCC	7 cut(s)	55, 410, 603, 673, 1015, 2009, 2593
XceI	RCATGY	4 cut(s)	256, 1055, 2120, 2456
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2603
XhoI	CTCGAG	1 cut(s)	2132
XmnI	GAANNNNTTC	3 cut(s)	2364, 2642, 3092
XspI	CTAG	7 cut(s)	1199, 1299, 1599, 1689, 2085, 2958, 2975
Zsp2I	ATGCAT	1 cut(s)	3214

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.