Rroxscaffold_7G00189850

Belongs to the ubiquitin-conjugating enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0005883
Physical Location & Seq
Forward (+)
29670924 .. 29678320
7397 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00189850.1

Sequence Viewer

Length: 3441 bp
ATGGGAAGCAAGCAGCATGACAGTGTTTTTAAAGACGATGAACCTACTACTGTAGACCATGATAATAGTTCTTTGATCCAGGTTGAGTATTCAACTGATTCAAATGTGAATAATGAATTTAAAAAGTTGAGTGAGGTTGTCGGTGGCATGAATAATACTCCATACATATATAGACAAGATGTTGTGAGGAGCAAGAATGGTATGATTGGGGTTGTGACTGAAGTTGCTGGTGATTCCGATTCTGATAGTGGCATGTCTGATGATGAGGATGAGGATGATGATGAAGAAGATGATGATGACCACGACAGTGAGAATCATGAAAATGACAAAGATAGTGGTGATGACAGTAATACTAATGAAAATGGTGACAAGAACCAGAACGGTGGTAATGATAAGACTGGTCCTCTTCCTGCTGACCAGGTTCGAGTGCTTTGGATAGATGAAACTGAGTCAACCCAGAATATCAATGATTTAACGGTTGTTGATCGAGGATTCTTGCATGGAGATTTTGTTGCTGCTGCTTCTGAGCCAACCGGGCAAGTTGGTGTCGTGGTGGATGTCAATATCTCTGTTGATTTGAAAGCTCCTGATGGGTCCATCATAAAAGATGTGTCATCCAATGACTTAAAACGTGTGAGGGAGTTCACTGTCGGTGATTACGTGGTCCTTGGTACCTGGCTGGGTAGAATTGAGGATGTTTTTGATAATGTGACAGTGATGATTGATGATGGTTCCCTATGTAGAATTTTGAGAGCAGACCCGATGGATCTTAAACCACTTTCCAAAAACATGCTTGAAGATGTGCATTTCCCTTATTACCCAGGCCAGCGTGTGAAGGCAAGGTCATCATCCGTTTTCAAGAATTCTTTGTGGTTATCTGGCTCATGGAAGGCTAATCGTCTGGAAGGTACTGTTACTAAAGTTACAGTGGGATCAGTGTTTATTTATTGGATAGCTTCTGCTGGCTGTGGCCCAGATTCTTCTACTGCCCCTGCTAAAGAGCAGGTCCCCAGGAAGTTGAAACTGTTGTCTTGTTTCACACATGCAAATTGGCAGTTGGGCGACTGGTGTCTTTTTCCATCATCAGGGTTGTCATCTTCCACTGCTGTAGATAAGGGTTCAAAATTAGAACTTCATCAATTAGATTCTACTCAAATGGGTAATGTGTGTGATTCGGAAGAGAGTTCACTAGAAGAATCAAACGGAAATAGTGCATCCATGGATACTGATCCAGTGTCTGTATTAGATGGAAACAATGGAAATGCTGGAAGTAATACTTCTATTGAATCTACTTCCAGCGGTAGTTCATTGTCAGTCACAAAGGTGCCTGTTTCCCTTCATCGCAAAAAGCTGCGTAAACCTGTGGTTAAAAGAGACAAGAAGGCACGAAAGGAGGAAAATTTTGAAAGATCTTTTTTGATTGCCAATACCAGGACAACAGTTGATGTGGCATGGCAGGATGGTTCAACAGAACGGAAGCTGGCTTCAACCAATTTAATTCCTCTTGATAGTCCTGGTGATCATGAATTTGTTGCTGAACAATATGTGGTGGAGAAGGCATCTGATGATGGTGATGGTGCTTTTGAAGATAGGCGTGTTGGGCTCGTGAAAAGCGTCAATGCCAAGGAGCGAACAGCTTGTGTACAGTGGTTGAAGGCAGTTTCCAGAGCTGAAGATCCTAGAGAGTTTGACAAGGAGGAAGTTGTTAGTGTATATGAGCTCGAGGGTCACCCAGATTATGATTACTGTTATGGGGATGTTGTTGTCAGATTGTTGCCAGTTTCTGTTTCTGCACAAACTGCTTCGGTATCGGACTTAATTGAGGAGCCAAAGCAGCAAAATCAATCAAGCGAGCTTACATCAGAAGTTGTTGATGAAGCTTCTTCTGTTGATAAATCTTGTATGGACTTCTCAGATCTTTCTTGGGTTGGGAATATAACTGGTCTGAAGAATGGTGATATTGAAGTTACATGGGCTGACGGGATGGTTTCTACGGTTGGACCTCAAGCAATTTATGTTGTTGGTCGAGCTGATGATGATGGCTCAATTGCTGGGAGCGAGGTTAGTGATGCTGCTAGTTGGGAAACAGTTGATGATGAGGAGGTGCATACCCTATTTCCCCTCGAGAGTATAACAGAGCAAGTGTTTGATGTTCAGGAAGTGGAGATACAGAGTGCCTTCAACATGAATTCTGAAGCAGAGGTGGGTGGAGAAGATAATTCTGGAAGGAACCCTGCATTGTCTGTTCCCTTGGCTGCACTTCGCTTTGTTACCAGACTGGCAAGTGGTATATTTGCTCGGGGACAAAAGAACTTGGATCCAATTTTAGATACTAAAGGTGAAGGTGATTTAGAGCCTCGGGAAGTTGAAATTTTTCAGGGAGATCATGGTGAAGATTCCAGCTCTCAGAAATCTAAAGTTGTTGATAGTAGTGTGGAGATCAATCATAAAAATGAAGAGCATGTTTCCTCGGAGACAACAAAAATATTGGATGCAGCAGAAATCTTGTGCAACTTAAGGACTGAAGGATCGGATGCTAAAGAAGTTAGCAAGGATGATGTTTGCAGTTTCAAACGCTTTGATATAGTCAAAGATCCTTTGGACCATCATTACCTTGGTGCAGCAGGGCAGGATGACTTCTCTAAATCCTACCTTGCATCATTACAGAATAGCAGTGGTAAAAAGTGGTTCAAGAAGATTCAGCAAGACTGGGGCATCCTTCAAAATAACCTGCCTGATGGCATCTACGTACGCGTTTATGAAGATCGAATGGATCTCTTGAGGGCAGTTATTGTTGGGGCTTATGGGACACCTTACCAAGATGGCCTCTTCTTTTTTGATTTTCACCTCCCTCCGGAGTACCCTGATGTCCCTCCGTCAGCATATTATCATTCTGGTGGCTGGCGGATAAATCCAAATCTATACGAAGAAGGCAAAGTCTGTCTTAGTCTGTTAAATACTTGGACTGGCAGAGGAAATGAAGTATGGGATCCAAAGTCTTCTAGCATCCTTCAGGTTCTAGTTTCACTCCAGGGATTGGTACTAAATTCTAAGCCCTATTTCAACGAAGCTGGCTATGATAAGCAAATTGGAACAGCTGAAGGGGAGAAAAATTCATTGTCCTACAATGAGAATACTTTCTTGCTGAATTGCAAGACGATAATGTCTCTTATGCGGAGGCCTCCCAAGGACTTTGAAGAGCTTGTTCAAGACCATTTCAGGAGGCGTGGTTATTATATTCTCAAGGCTTGCGATGCATATATGAAAGGCTACTTGATAGGTTCGCTCACTAAAGATGCCTCTGTTAGTGATAGCAGTGATGCAAATTCGACCTCTGTTGGTTTCAAATTGATGTTAGCTAAGATTGTGCCAAAGCTGTTCTTGGCTTTGAGTGAGGTTGGAGCTGATTGTCATGAATTTAAGCATCTGCAACAGTCATAG

Protein Analysis

1146

Amino Acids

126.19

Weight (kDa)

4.56

Isoelectric Point (pI)

36.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SH3-A_UBE2O PF23048 56 - 161 1.5e-06 UBE2O, N-terminal SH3-A domain
tSH3-B_UBE2O PF23046 217 - 348 1.7e-42 UBE2O, tandem tSH3-B domain
SH3-B_UBE2O PF23043 507 - 579 6.1e-20 UBE2O, SH3-B domain
SH3-C_UBE2O PF23044 638 - 678 3.3e-06 UBE2O, ubiquitin binding SH3-C domain
UQ_con PF00179 900 - 1032 8.6e-20 Ubiquitin-conjugating enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 3163
Acc36I ACCTGC 2 cut(s) 994, 2739
Acc65I GGTACC 1 cut(s) 671
AccB1I GGYRCC 2 cut(s) 671, 1324
AccB7I CCANNNNNTGG 1 cut(s) 3037
AccI GTMKAC 1 cut(s) 54
AccII CGCG 1 cut(s) 2754
AccIII TCCGGA 1 cut(s) 2854
AciI CCGC 3 cut(s) 1299, 2905, 3175
AcuI CTGAAG 7 cut(s) 240, 1692, 1967, 2214, 2544, 2996, 3120
AfaI GTAC 6 cut(s) 673, 910, 1644, 2751, 2861, 3042
AfiI CCNNNNNNNGG 4 cut(s) 1085, 1431, 2854, 3037
AflII CTTAAG 1 cut(s) 2515
AflIII ACRYGT 2 cut(s) 631, 2752
AjnI CCWGG 8 cut(s) 78, 417, 674, 820, 1010, 1430, 1513, 3030
AleI CACNNNNGTG 2 cut(s) 306, 1322
Alw21I GWGCWC 1 cut(s) 1722
Alw26I GTCTC 3 cut(s) 1368, 2468, 3171
AlwNI CAGNNNCTG 2 cut(s) 1238, 1784
Ama87I CYCGRG 4 cut(s) 1721, 2123, 2298, 2358
Aor13HI TCCGGA 1 cut(s) 2854
AoxI GGCC 4 cut(s) 823, 970, 2824, 3179
ApeKI GCWGC 9 cut(s) 13, 515, 518, 1351, 1834, 2072, 2255, 2495, 2621
Asp700I GAANNNNTTC 2 cut(s) 2373, 3137
Asp718I GGTACC 1 cut(s) 671
AspS9I GGNCC 7 cut(s) 401, 594, 664, 971, 1006, 2000, 2602
AsuC2I CCSGG 1 cut(s) 535
AvaI CYCGRG 4 cut(s) 1721, 2123, 2298, 2358
AvaII GGWCC 6 cut(s) 401, 594, 664, 1006, 2000, 2602
BamHI GGATCC 2 cut(s) 2317, 2989
BanI GGYRCC 2 cut(s) 671, 1324
BanII GRGCYC 2 cut(s) 1605, 1722
BarI GAAGNNNNNNTAC 2 cut(s) 2151, 2183
BauI CACGAG 1 cut(s) 1604
BbsI GAAGAC 1 cut(s) 2991
Bbv12I GWGCWC 1 cut(s) 1722
BbvI GCAGC 9 cut(s) 25, 502, 505, 1338, 1846, 2059, 2242, 2507, 2633
BciT130I CCWGG 8 cut(s) 80, 419, 676, 822, 1012, 1432, 1515, 3032
BciVI GTATCC 1 cut(s) 1216
BclI TGATCA 1 cut(s) 1519
BcnI CCSGG 1 cut(s) 535
BcoDI GTCTC 3 cut(s) 1368, 2468, 3171
BfaI CTAG 5 cut(s) 1190, 1680, 2076, 3003, 3020
BfmI CTRYAG 2 cut(s) 51, 1107
BfrI CTTAAG 1 cut(s) 2515
BfuAI ACCTGC 2 cut(s) 994, 2739
BfuI GTATCC 1 cut(s) 1216
BglI GCCNNNNNGGC 1 cut(s) 535
BglII AGATCT 2 cut(s) 1409, 1915
BisI GCNGC 9 cut(s) 14, 516, 519, 1352, 1835, 2073, 2256, 2496, 2622
BlsI GCNGC 9 cut(s) 15, 517, 520, 1353, 1836, 2074, 2257, 2497, 2623
Bme1390I CCNGG 9 cut(s) 80, 419, 535, 676, 822, 1012, 1432, 1515, 3032
Bme18I GGWCC 6 cut(s) 401, 594, 664, 1006, 2000, 2602
BmeT110I CYCGRG 4 cut(s) 1721, 2123, 2298, 2358
BmgT120I GGNCC 7 cut(s) 401, 594, 664, 971, 1006, 2000, 2602
BmiI GGNNCC 9 cut(s) 595, 673, 733, 1008, 1326, 1827, 2231, 2319, 2991
BmrFI CCNGG 9 cut(s) 80, 419, 535, 676, 822, 1012, 1432, 1515, 3032
BmrI ACTGGG 1 cut(s) 2719
BmuI ACTGGG 1 cut(s) 2719
BoxI GACNNNNGTC 1 cut(s) 1068
BpiI GAAGAC 1 cut(s) 2991
BpmI CTGGAG 1 cut(s) 3014
BpuEI CTTGAG 3 cut(s) 1989, 2800, 3227
BpuMI CCSGG 1 cut(s) 535
BsaAI YACGTR 2 cut(s) 661, 2749
BsaBI GATNNNNATC 1 cut(s) 1227
BsaWI WCCGGW 1 cut(s) 2854
BsaXI ACNNNNNCTCC 4 cut(s) 495, 525, 3214, 3244
Bsc4I CCNNNNNNNGG 4 cut(s) 1085, 1431, 2854, 3037
Bse1I ACTGG 8 cut(s) 403, 1070, 1232, 1778, 1945, 2283, 2714, 2971
Bse8I GATNNNNATC 1 cut(s) 1227
BseAI TCCGGA 1 cut(s) 2854
BseBI CCWGG 8 cut(s) 80, 419, 676, 822, 1012, 1432, 1515, 3032
BseJI GATNNNNATC 1 cut(s) 1227
BseLI CCNNNNNNNGG 4 cut(s) 1085, 1431, 2854, 3037
BseMII CTCAG 4 cut(s) 438, 516, 1926, 2420
BseNI ACTGG 8 cut(s) 403, 1070, 1232, 1778, 1945, 2283, 2714, 2971
BseRI GAGGAG 3 cut(s) 202, 1838, 2114
BseXI GCAGC 9 cut(s) 25, 502, 505, 1338, 1846, 2059, 2242, 2507, 2633
BseYI CCCAGC 2 cut(s) 679, 2051
BsgI GTGCAG 3 cut(s) 1776, 2241, 2640
Bsh1236I CGCG 1 cut(s) 2754
BshFI GGCC 4 cut(s) 825, 972, 2826, 3181
BshNI GGYRCC 2 cut(s) 671, 1324
BsiHKAI GWGCWC 1 cut(s) 1722
BsiHKCI CYCGRG 4 cut(s) 1721, 2123, 2298, 2358
BsiSI CCGG 2 cut(s) 534, 2855
BsiWI CGTACG 1 cut(s) 2749
BslFI GGGAC 4 cut(s) 992, 2316, 2821, 2855
BslI CCNNNNNNNGG 4 cut(s) 1085, 1431, 2854, 3037
BsmAI GTCTC 3 cut(s) 1368, 2468, 3171
BsmFI GGGAC 4 cut(s) 992, 2316, 2821, 2855
BsnI GGCC 4 cut(s) 825, 972, 2826, 3181
BsoBI CYCGRG 4 cut(s) 1721, 2123, 2298, 2358
Bsp1286I GDGCHC 2 cut(s) 1605, 1722
Bsp13I TCCGGA 1 cut(s) 2854
Bsp1407I TGTACA 1 cut(s) 1642
Bsp19I CCATGG 1 cut(s) 1218
BspACI CCGC 3 cut(s) 1299, 2905, 3175
BspANI GGCC 4 cut(s) 825, 972, 2826, 3181
BspCNI CTCAG 4 cut(s) 439, 517, 1925, 2419
BspEI TCCGGA 1 cut(s) 2854
BspFNI CGCG 1 cut(s) 2754
BspHI TCATGA 3 cut(s) 316, 1522, 3412
BspLI GGNNCC 9 cut(s) 595, 673, 733, 1008, 1326, 1827, 2231, 2319, 2991
BspMI ACCTGC 2 cut(s) 994, 2739
BspQI GCTCTTC 2 cut(s) 2451, 3192
BspT107I GGYRCC 2 cut(s) 671, 1324
BspTI CTTAAG 1 cut(s) 2515
BsrGI TGTACA 1 cut(s) 1642
BsrI ACTGG 8 cut(s) 403, 1070, 1232, 1778, 1945, 2283, 2714, 2971
BssSI CACGAG 1 cut(s) 1604
BssT1I CCWWGG 6 cut(s) 667, 1218, 1623, 2250, 2614, 3186
Bst2BI CACGAG 1 cut(s) 1604
Bst2UI CCWGG 8 cut(s) 80, 419, 676, 822, 1012, 1432, 1515, 3032
Bst6I CTCTTC 5 cut(s) 411, 1173, 2451, 2834, 3192
BstAFI CTTAAG 1 cut(s) 2515
BstAPI GCANNNNNTGC 1 cut(s) 1799
BstAUI TGTACA 1 cut(s) 1642
BstBAI YACGTR 2 cut(s) 661, 2749
BstC8I GCNNGC 8 cut(s) 11, 827, 964, 1482, 1853, 2903, 3073, 3250
BstDEI CTNAG 7 cut(s) 447, 525, 1912, 2406, 2945, 3051, 3360
BstDSI CCRYGG 1 cut(s) 1218
BstEII GGTNACC 1 cut(s) 1727
BstFNI CGCG 1 cut(s) 2754
BstMAI GTCTC 3 cut(s) 1368, 2468, 3171
BstMWI GCNNNNNNNGC 5 cut(s) 535, 1600, 1799, 1834, 3254
BstNI CCWGG 8 cut(s) 80, 419, 676, 822, 1012, 1432, 1515, 3032
BstNSI RCATGY 4 cut(s) 256, 793, 1046, 2465
BstPAI GACNNNNGTC 1 cut(s) 1068
BstPI GGTNACC 1 cut(s) 1727
BstSCI CCNGG 9 cut(s) 78, 417, 533, 674, 820, 1010, 1430, 1513, 3030
BstSFI CTRYAG 2 cut(s) 51, 1107
BstSNI TACGTA 1 cut(s) 2749
BstUI CGCG 1 cut(s) 2754
BstV1I GCAGC 9 cut(s) 25, 502, 505, 1338, 1846, 2059, 2242, 2507, 2633
BstV2I GAAGAC 1 cut(s) 2991
BstX2I RGATCY 8 cut(s) 766, 1409, 1675, 1915, 2317, 2593, 2773, 2989
BstXI CCANNNNNNTGG 1 cut(s) 383
BstYI RGATCY 8 cut(s) 766, 1409, 1675, 1915, 2317, 2593, 2773, 2989
BsuI GTATCC 1 cut(s) 1216
BsuRI GGCC 4 cut(s) 825, 972, 2826, 3181
BtgI CCRYGG 1 cut(s) 1218
BtgZI GCGATG 2 cut(s) 1325, 3267
BtsI GCAGTG 3 cut(s) 1101, 2680, 3322
BveI ACCTGC 2 cut(s) 994, 2739
Cac8I GCNNGC 8 cut(s) 11, 827, 964, 1482, 1853, 2903, 3073, 3250
CaiI CAGNNNCTG 2 cut(s) 1238, 1784
CciI TCATGA 3 cut(s) 316, 1522, 3412
Cfr13I GGNCC 7 cut(s) 401, 594, 664, 971, 1006, 2000, 2602
CseI GACGC 1 cut(s) 1603
CsiI ACCWGGT 1 cut(s) 417
Csp6I GTAC 6 cut(s) 672, 909, 1643, 2750, 2860, 3041
CspCI CAANNNNNGTGG 2 cut(s) 316, 351
CviQI GTAC 6 cut(s) 672, 909, 1643, 2750, 2860, 3041
DdeI CTNAG 7 cut(s) 447, 525, 1912, 2406, 2945, 3051, 3360
DraI TTTAAA 2 cut(s) 31, 121
DrdI GACNNNNNNGTC 1 cut(s) 3163
DseDI GACNNNNNNGTC 1 cut(s) 3163
Eam1104I CTCTTC 5 cut(s) 411, 1173, 2451, 2834, 3192
EarI CTCTTC 5 cut(s) 411, 1173, 2451, 2834, 3192
EciI GGCGGA 1 cut(s) 2920
Ecl136II GAGCTC 1 cut(s) 1720
Eco105I TACGTA 1 cut(s) 2749
Eco130I CCWWGG 6 cut(s) 667, 1218, 1623, 2250, 2614, 3186
Eco147I AGGCCT 1 cut(s) 3181
Eco24I GRGCYC 2 cut(s) 1605, 1722
Eco47I GGWCC 6 cut(s) 401, 594, 664, 1006, 2000, 2602
Eco53kI GAGCTC 1 cut(s) 1720
Eco57I CTGAAG 7 cut(s) 240, 1692, 1967, 2214, 2544, 2996, 3120
Eco88I CYCGRG 4 cut(s) 1721, 2123, 2298, 2358
Eco91I GGTNACC 1 cut(s) 1727
EcoICRI GAGCTC 1 cut(s) 1720
EcoO109I RGGNCCY 1 cut(s) 1006
EcoO65I GGTNACC 1 cut(s) 1727
EcoRI GAATTC 2 cut(s) 862, 2188
EcoRII CCWGG 8 cut(s) 78, 417, 674, 820, 1010, 1430, 1513, 3030
EcoT14I CCWWGG 6 cut(s) 667, 1218, 1623, 2250, 2614, 3186
EcoT22I ATGCAT 1 cut(s) 3259
EcoT38I GRGCYC 2 cut(s) 1605, 1722
ErhI CCWWGG 6 cut(s) 667, 1218, 1623, 2250, 2614, 3186
FalI AAGNNNNNCTT 6 cut(s) 1261, 1293, 2928, 2960, 3365, 3397
FaqI GGGAC 4 cut(s) 992, 2316, 2821, 2855
FbaI TGATCA 1 cut(s) 1519
FblI GTMKAC 1 cut(s) 54
Fnu4HI GCNGC 9 cut(s) 14, 516, 519, 1352, 1835, 2073, 2256, 2496, 2622
FriOI GRGCYC 2 cut(s) 1605, 1722
Fsp4HI GCNGC 9 cut(s) 14, 516, 519, 1352, 1835, 2073, 2256, 2496, 2622
FspBI CTAG 5 cut(s) 1190, 1680, 2076, 3003, 3020
GluI GCNGC 9 cut(s) 14, 516, 519, 1352, 1835, 2073, 2256, 2496, 2622
GsaI CCCAGC 2 cut(s) 683, 2055
GsuI CTGGAG 1 cut(s) 3014
HaeIII GGCC 4 cut(s) 825, 972, 2826, 3181
HapII CCGG 2 cut(s) 534, 2855
HgaI GACGC 1 cut(s) 1603
HincII GTYRAC 1 cut(s) 453
HindII GTYRAC 1 cut(s) 453
HindIII AAGCTT 1 cut(s) 1878
HpaII CCGG 2 cut(s) 534, 2855
Hpy166II GTNNAC 6 cut(s) 55, 453, 645, 1187, 1358, 1643
Hpy8I GTNNAC 6 cut(s) 55, 453, 645, 1187, 1358, 1643
HpyCH4IV ACGT 3 cut(s) 631, 660, 2748
HpyF10VI GCNNNNNNNGC 5 cut(s) 535, 1600, 1799, 1834, 3254
HpyF3I CTNAG 7 cut(s) 447, 525, 1912, 2406, 2945, 3051, 3360
HpySE526I ACGT 3 cut(s) 631, 660, 2748
Kpn2I TCCGGA 1 cut(s) 2854
KpnI GGTACC 1 cut(s) 675
Ksp22I TGATCA 1 cut(s) 1519
LguI GCTCTTC 2 cut(s) 2451, 3192
LmnI GCTCC 6 cut(s) 189, 589, 1627, 1825, 2055, 3401
Lsp1109I GCAGC 9 cut(s) 25, 502, 505, 1338, 1846, 2059, 2242, 2507, 2633
MabI ACCWGGT 1 cut(s) 417
MaeI CTAG 5 cut(s) 1190, 1680, 2076, 3003, 3020
MaeII ACGT 3 cut(s) 631, 660, 2748
MaeIII GTNAC 9 cut(s) 214, 365, 709, 913, 922, 1315, 1727, 1966, 2269
MfeI CAATTG 1 cut(s) 2046
MflI RGATCY 8 cut(s) 766, 1409, 1675, 1915, 2317, 2593, 2773, 2989
MhlI GDGCHC 2 cut(s) 1605, 1722
MluI ACGCGT 1 cut(s) 2752
MlyI GAGTC 1 cut(s) 458
MmeI TCCRAC 2 cut(s) 1978, 3379
Mph1103I ATGCAT 1 cut(s) 3259
MroI TCCGGA 1 cut(s) 2854
MroXI GAANNNNTTC 2 cut(s) 2373, 3137
MslI CAYNNNNRTG 6 cut(s) 21, 306, 321, 1322, 2451, 2895
MspA1I CMGCKG 2 cut(s) 1299, 3098
MspCI CTTAAG 1 cut(s) 2515
MspI CCGG 2 cut(s) 534, 2855
MspR9I CCNGG 9 cut(s) 80, 419, 535, 676, 822, 1012, 1432, 1515, 3032
MunI CAATTG 1 cut(s) 2046
MvaI CCWGG 8 cut(s) 80, 419, 676, 822, 1012, 1432, 1515, 3032
MvnI CGCG 1 cut(s) 2754
MwoI GCNNNNNNNGC 5 cut(s) 535, 1600, 1799, 1834, 3254
NciI CCSGG 1 cut(s) 535
NcoI CCATGG 1 cut(s) 1218
NlaIV GGNNCC 9 cut(s) 595, 673, 733, 1008, 1326, 1827, 2231, 2319, 2991
NmuCI GTSAC 5 cut(s) 214, 365, 709, 1315, 1727
NsiI ATGCAT 1 cut(s) 3259
NspI RCATGY 4 cut(s) 256, 793, 1046, 2465
OliI CACNNNNGTG 2 cut(s) 306, 1322
PaeR7I CTCGAG 2 cut(s) 1721, 2123
PagI TCATGA 3 cut(s) 316, 1522, 3412
PceI AGGCCT 1 cut(s) 3181
PciSI GCTCTTC 2 cut(s) 2451, 3192
PcsI WCGNNNNNNNCGW 2 cut(s) 657, 1611
PdmI GAANNNNTTC 2 cut(s) 2373, 3137
Pfl23II CGTACG 1 cut(s) 2749
PflMI CCANNNNNTGG 1 cut(s) 3037
PkrI GCNGC 9 cut(s) 15, 517, 520, 1353, 1836, 2074, 2257, 2497, 2623
PleI GAGTC 1 cut(s) 457
PpsI GAGTC 1 cut(s) 457
Ppu21I YACGTR 2 cut(s) 661, 2749
PpuMI RGGWCCY 1 cut(s) 1006
PshAI GACNNNNGTC 1 cut(s) 1068
Psp124BI GAGCTC 1 cut(s) 1722
Psp5II RGGWCCY 1 cut(s) 1006
Psp6I CCWGG 8 cut(s) 78, 417, 674, 820, 1010, 1430, 1513, 3030
PspEI GGTNACC 1 cut(s) 1727
PspFI CCCAGC 2 cut(s) 679, 2051
PspGI CCWGG 8 cut(s) 78, 417, 674, 820, 1010, 1430, 1513, 3030
PspLI CGTACG 1 cut(s) 2749
PspN4I GGNNCC 9 cut(s) 595, 673, 733, 1008, 1326, 1827, 2231, 2319, 2991
PspPI GGNCC 7 cut(s) 401, 594, 664, 971, 1006, 2000, 2602
PspPPI RGGWCCY 1 cut(s) 1006
PspXI VCTCGAGB 1 cut(s) 1721
PstNI CAGNNNCTG 2 cut(s) 1238, 1784
PsuI RGATCY 8 cut(s) 766, 1409, 1675, 1915, 2317, 2593, 2773, 2989
PvuII CAGCTG 1 cut(s) 3098
RsaI GTAC 6 cut(s) 673, 910, 1644, 2751, 2861, 3042
RsaNI GTAC 6 cut(s) 672, 909, 1643, 2750, 2860, 3041
RseI CAYNNNNRTG 6 cut(s) 21, 306, 321, 1322, 2451, 2895
SacI GAGCTC 1 cut(s) 1722
SapI GCTCTTC 2 cut(s) 2451, 3192
SatI GCNGC 9 cut(s) 14, 516, 519, 1352, 1835, 2073, 2256, 2496, 2622
Sau96I GGNCC 7 cut(s) 401, 594, 664, 971, 1006, 2000, 2602
SchI GAGTC 1 cut(s) 458
ScrFI CCNGG 9 cut(s) 80, 419, 535, 676, 822, 1012, 1432, 1515, 3032
SduI GDGCHC 2 cut(s) 1605, 1722
SexAI ACCWGGT 1 cut(s) 417
SfcI CTRYAG 2 cut(s) 51, 1107
Sfr274I CTCGAG 2 cut(s) 1721, 2123
SinI GGWCC 6 cut(s) 401, 594, 664, 1006, 2000, 2602
SlaI CTCGAG 2 cut(s) 1721, 2123
SmiMI CAYNNNNRTG 6 cut(s) 21, 306, 321, 1322, 2451, 2895
SmlI CTYRAG 6 cut(s) 1721, 2004, 2123, 2515, 2779, 3242
SmoI CTYRAG 6 cut(s) 1721, 2004, 2123, 2515, 2779, 3242
SnaBI TACGTA 1 cut(s) 2749
SseBI AGGCCT 1 cut(s) 3181
SsiI CCGC 3 cut(s) 1299, 2905, 3175
SspI AATATT 1 cut(s) 2487
SspMI CTAG 5 cut(s) 1190, 1680, 2076, 3003, 3020
SstI GAGCTC 1 cut(s) 1722
StuI AGGCCT 1 cut(s) 3181
StyD4I CCNGG 9 cut(s) 78, 417, 533, 674, 820, 1010, 1430, 1513, 3030
StyI CCWWGG 6 cut(s) 667, 1218, 1623, 2250, 2614, 3186
TaiI ACGT 3 cut(s) 634, 663, 2751
TaqI TCGA 7 cut(s) 424, 487, 1722, 2026, 2124, 2767, 3329
TatI WGTACW 1 cut(s) 1642
TseFI GTSAC 5 cut(s) 214, 365, 709, 1315, 1727
TseI GCWGC 9 cut(s) 13, 515, 518, 1351, 1834, 2072, 2255, 2495, 2621
Tsp45I GTSAC 5 cut(s) 214, 365, 709, 1315, 1727
TspGWI ACGGA 4 cut(s) 841, 1218, 1489, 2865
Van91I CCANNNNNTGG 1 cut(s) 3037
Vha464I CTTAAG 1 cut(s) 2515
VpaK11BI GGWCC 6 cut(s) 401, 594, 664, 1006, 2000, 2602
XceI RCATGY 4 cut(s) 256, 793, 1046, 2465
XcmI CCANNNNNNNNNTGG 1 cut(s) 2612
XhoI CTCGAG 2 cut(s) 1721, 2123
XmiI GTMKAC 1 cut(s) 54
XmnI GAANNNNTTC 2 cut(s) 2373, 3137
XspI CTAG 5 cut(s) 1190, 1680, 2076, 3003, 3020
Zsp2I ATGCAT 1 cut(s) 3259
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.