Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000007

Orthogroup ID

OG0005883

Physical Location & Seq

Genomic Coordinates Forward (+)

30431473 .. 30438677

Gene Structure 7205 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_7G00190340.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3135 bp

ATGTCTGATGATGAGGATGAGGATGATGATGAAGAAGATGATGATGACCACGACAGTGAGAATCATGAAAATGACAAAGATAGTGGTGATGACAGTAATACTAATGAAAATGGTGACAAGAACCAGAACGGTGGTAATGATAAGACTGGTCCTCTTCCTGCTGACCAGGTTCGAGTGCTTTGGATAGATGAAACTGAGTCAACCCAGAATATCAATGATTTAACGGTTGTTGATCGAGGATTCTTGCATGGAGATTTTGTTGCTGCTGCTTCTGAGCCAACCGGGCAAGTTGGTGTCGTGGTGGATGTCAATATCTCTGTTGATTTGAAAGCTCCTGATGGGTCCATCATAAAAGATGTGTCATCCAATGACTTAAAACGTGTGAGGGAGTTCACTGTCGGTGATTACGTGGTCCTTGGTACCTGGCTGGGTAGAATTGAGGATGTTTTTGATAATGTGACAGTGATGATTGATGATGGTTCCCTATGTAGAATTTTGAGAGCAGACCCGATGGATCTTAAACCACTTTCCAAAAACATGCTTGAAGATGTGCATTTCCCTTATTACCCAGGCCAGCGTGTGAAGGCAAGGTCATCATCCGTTTTCAAGAATTCTTTGTGGTTATCTGGCTCATGGAAGGCTAATCGTCTGGAAGGTACTGTTACTAAAGTTACAGTGGGATCAGTGTTTATTTATTGGATAGCTTCTGCTGGCTGTGGCCCAGATTCTTCTACTGCCCCTGCTAAAGAGCAGGTCCCCAGGAAGTTGAAACTGTTGTCTTGTTTCACACATGCAAATTGGCAGTTGGGCGACTGGTGTCTTTTTCCATCATCAGGGTTGTCATCTTCCACTGCTGTAGATAAGGGTTCAAAATTAGAACTTCATCAATTAGATTCTACTCAAATGGGTAATGTGTGTGATTCGGAAGAGAGTTCACTAGAAGAATCAAACGGAAATAGTGCATCCATGGATACTGATCCAGTGTCTGTATTAGATGGAAACAATGGAAATGCTGGAAGTAATACTTCTATTGAATCTACTTCCAGCGGTAGTTCATTGTCAGTCACAAAGGTGCCTGTTTCCCTTCATCGCAAAAAGCTGCGTAAACCTGTGGTTAAAAGAGACAAGAAGGCACGAAAGGAGGAAAATTTTGAAAGATCTTTTTTGATTGCCAATACCAGGACAACAGTTGATGTGGCATGGCAGGATGGTTCAACAGAACGGAAGCTGGCTTCAACCAATTTAATTCCTCTTGATAGTCCTGGTGATCATGAATTTGTTGCTGAACAATATGTGGTGGAGAAGGCATCTGATGATGGTGATGGTGCTTTTGAAGATAGGCGTGTTGGGCTCGTGAAAAGCGTCAATGCCAAGGAGCGAACAGCTTGTGTACAGTGGTTGAAGGCAGTTTCCAGAGCTGAAGATCCTAGAGAGTTTGACAAGGAGGAAGTTGTTAGTGTATATGAGCTCGAGGGTCACCCAGATTATGATTACTGTTATGGGGATGTTGTTGTCAGATTGTTGCCAGTTTCTGTTTCTGCACAAACTGCTTCGGTATCGGACTTAATTGAGGAGCCAAAGCAGCAAAATCAATCAAGCGAGCTTACATCAGAAGTTGTTGATGAAGCTTCTTCTGTTGATAAATCTTGTATGGACTTCTCAGATCTTTCTTGGGTTGGGAATATAACTGGTCTGAAGAATGGTGATATTGAAGTTACATGGGCTGACGGGATGGTTTCTACGGTTGGACCTCAAGCAATTTATGTTGTTGGTCGAGCTGATGATGATGGCTCAATTGCTGGGAGCGAGGTTAGTGATGCTGCTAGTTGGGAAACAGTTGATGATGAGGAGGTGCATACCCTATTTCCCCTCGAGAGTATAACAGAGGAAGTGGAGATACAGAGTGCCTTCAACATGAATTCTGAAGCAGAGGTGGGTGGAGAAGATAATTCTGGAAGGAACCCTGCATTGTCTGTTCCCTTGGCTGCACTTCGCTTTGTTACCAGACTGGCAAGTGGTATATTTGCTCGGGGACAAAAGAACTTGGATCCAATTTTAGATACTAAAGGTGAAGGTGATTTAGAGCCTCGGGAAGTTGAAATTTTTCAGGGAGATCATGGTGAAGATTCCAGCTCTCAGAAATCTAAAGTTGTTGATAGTAGTGTGGAGATCAATCATAAAAATGAAGAGCATGTTTCCTCGGAGACAACAAAAATATTGGATGCAGCAGAAATCTTGTGCAACTTAAGGACTGAAGGATCGGATGCTAAAGAAGTTAGCAAGGATGATGTTTGCAGTTTCAAACGCTTTGATATAGTCAAAGATCCTTTGGACCATCATTACCTTGGTGCAGCAGGGCAGAATAGCAGTGGTAAAAAGTGGTTCAAGAAGATTCAGCAAGACTGGGGCATCCTTCAAAATAACCTGCCTGATGGCATCTACGTACGCGTTTATGAAGATCGAATGGATCTCTTGAGGGCAGTTATTGTTGGGGCTTATGGGACACCTTACCAAGATGGCCTCTTCTTTTTTGATTTTCACCTCCCTCCGGAGTACCCTGATGTCCCTCCGTCAGCATATTATCATTCTGGTGGCTGGCGGATAAATCCAAATCTATACGAAGAAGGCAAAGTCTGTCTTAGTCTGTTAAATACTTGGACTGGCAGAGGAAATGAAGTATGGGATCCAAAGTCTTCTAGCATCCTTCAGGTTCTAGTTTCACTCCAGGGATTGGTACTAAATTCTAAGCCCTATTTCAACGAAGCTGGCTATGATAAGCAAATTGGAACAGCTGAAGGGGAGAAAAATTCATTGTCCTACAATGAGAATACTTTCTTGCTGAATTGCAAGACGATAATGTCTCTTATGCGGAGGCCTCCCAAGGACTTTGAAGAGCTTGTTCAAGACCATTTCAGGAGGCGTGGTTATCATATTCTCAAGGCTTGCGATGCATATATGAAAGGCTACTTGATAGGTTCGCTCACTAAAGATGCCTCTGTTAGTGATAGCAGTGATGCAAATTCGACCTCTGTTGGTTTCAAATTGATGTTAGCTAAGATTGTGCCAAAGCTGTTCTTGGCTTTGAGTGAGGTTGGAGCTGATTGTCATGAATTTAAGCATCTGCAACAGTCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006464 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019899 GO:0031625 GO:0032446 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044389 GO:0044424 GO:0044444 GO:0044464 GO:0061630 GO:0061659 GO:0070647 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04120 map04120

Pfam Domains

Protein Families

UQ_con

Protein Analysis

1044

Amino Acids

114.92

Weight (kDa)

4.61

Isoelectric Point (pI)

37.64

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SH3-A_UBE2O	PF23048	7 - 77	2.9e-06	UBE2O, N-terminal SH3-A domain
tSH3-B_UBE2O	PF23046	133 - 264	1.5e-42	UBE2O, tandem tSH3-B domain
SH3-B_UBE2O	PF23043	423 - 495	5.4e-20	UBE2O, SH3-B domain
SH3-C_UBE2O	PF23044	554 - 594	3e-06	UBE2O, ubiquitin binding SH3-C domain
UQ_con	PF00179	798 - 930	7.5e-20	Ubiquitin-conjugating enzyme

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0005883)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G16920 AT2G16920
fragaria_vesca	FvH4_2g15490 FvH4_2g15490 FvH4_2g15490 FvH4_2g15490 FvH4_2g15490 FvH4_2g15490 FvH4_2g15490
malus_domestica	MD05G1123200.v1.1 MD10G1126200.v1.1
prunus_persica	Prupe.8G167100_v2.0.a1 Prupe.8G167100_v2.0.a1 Prupe.8G167100_v2.0.a1
pyrus_communis	pycom05g11760 pycom10g10840
rosa_chinensis	RchiOBHm_Chr6g0278491
rosa_laevigata	RLG00000013224
rosa_multiflora	Rmu_sc0000258.1_g000006
rosa_roxburghii	Rroxscaffold_7G00189850 Rroxscaffold_7G00190340
rosa_rugosa	Rorug06G0117200 Rorug06G0117200
rosa_samantha	Rh6AG226500 Rh6BG230700 Rh6CG233800 Rh6DG225100
rosa_wichuraiana	Rw6G019770

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	2857
Acc36I	ACCTGC	2 cut(s)	742, 2433
Acc65I	GGTACC	1 cut(s)	419
AccB1I	GGYRCC	2 cut(s)	419, 1072
AccB7I	CCANNNNNTGG	1 cut(s)	2731
AccII	CGCG	1 cut(s)	2448
AccIII	TCCGGA	1 cut(s)	2548
AciI	CCGC	3 cut(s)	1047, 2599, 2869
AcuI	CTGAAG	6 cut(s)	1440, 1715, 1944, 2274, 2690, 2814
AfaI	GTAC	6 cut(s)	421, 658, 1392, 2445, 2555, 2736
AfiI	CCNNNNNNNGG	4 cut(s)	833, 1179, 2548, 2731
AflII	CTTAAG	1 cut(s)	2245
AflIII	ACRYGT	2 cut(s)	379, 2446
AjnI	CCWGG	7 cut(s)	165, 422, 568, 758, 1178, 1261, 2724
AleI	CACNNNNGTG	2 cut(s)	54, 1070
Alw21I	GWGCWC	1 cut(s)	1470
Alw26I	GTCTC	3 cut(s)	1116, 2198, 2865
AlwNI	CAGNNNCTG	2 cut(s)	986, 1532
Ama87I	CYCGRG	4 cut(s)	1469, 1871, 2028, 2088
Aor13HI	TCCGGA	1 cut(s)	2548
AoxI	GGCC	4 cut(s)	571, 718, 2518, 2873
ApeKI	GCWGC	8 cut(s)	263, 266, 1099, 1582, 1820, 1985, 2225, 2351
Asp700I	GAANNNNTTC	2 cut(s)	2103, 2831
Asp718I	GGTACC	1 cut(s)	419
AspS9I	GGNCC	7 cut(s)	149, 342, 412, 719, 754, 1748, 2332
AsuC2I	CCSGG	1 cut(s)	283
AvaI	CYCGRG	4 cut(s)	1469, 1871, 2028, 2088
AvaII	GGWCC	6 cut(s)	149, 342, 412, 754, 1748, 2332
BamHI	GGATCC	2 cut(s)	2047, 2683
BanI	GGYRCC	2 cut(s)	419, 1072
BanII	GRGCYC	2 cut(s)	1353, 1470
BarI	GAAGNNNNNNTAC	2 cut(s)	1881, 1913
BauI	CACGAG	1 cut(s)	1352
BbsI	GAAGAC	1 cut(s)	2685
Bbv12I	GWGCWC	1 cut(s)	1470
BbvI	GCAGC	8 cut(s)	250, 253, 1086, 1594, 1807, 1972, 2237, 2363
BciT130I	CCWGG	7 cut(s)	167, 424, 570, 760, 1180, 1263, 2726
BciVI	GTATCC	1 cut(s)	964
BclI	TGATCA	1 cut(s)	1267
BcnI	CCSGG	1 cut(s)	283
BcoDI	GTCTC	3 cut(s)	1116, 2198, 2865
BfaI	CTAG	5 cut(s)	938, 1428, 1824, 2697, 2714
BfmI	CTRYAG	1 cut(s)	855
BfrI	CTTAAG	1 cut(s)	2245
BfuAI	ACCTGC	2 cut(s)	742, 2433
BfuI	GTATCC	1 cut(s)	964
BglI	GCCNNNNNGGC	1 cut(s)	283
BglII	AGATCT	2 cut(s)	1157, 1663
BisI	GCNGC	8 cut(s)	264, 267, 1100, 1583, 1821, 1986, 2226, 2352
BlsI	GCNGC	8 cut(s)	265, 268, 1101, 1584, 1822, 1987, 2227, 2353
Bme1390I	CCNGG	8 cut(s)	167, 283, 424, 570, 760, 1180, 1263, 2726
Bme18I	GGWCC	6 cut(s)	149, 342, 412, 754, 1748, 2332
BmeT110I	CYCGRG	4 cut(s)	1469, 1871, 2028, 2088
BmgT120I	GGNCC	7 cut(s)	149, 342, 412, 719, 754, 1748, 2332
BmiI	GGNNCC	9 cut(s)	343, 421, 481, 756, 1074, 1575, 1961, 2049, 2685
BmrFI	CCNGG	8 cut(s)	167, 283, 424, 570, 760, 1180, 1263, 2726
BmrI	ACTGGG	1 cut(s)	2413
BmuI	ACTGGG	1 cut(s)	2413
BoxI	GACNNNNGTC	1 cut(s)	816
BpiI	GAAGAC	1 cut(s)	2685
BpmI	CTGGAG	1 cut(s)	2708
BpuEI	CTTGAG	3 cut(s)	1737, 2494, 2921
BpuMI	CCSGG	1 cut(s)	283
BsaAI	YACGTR	2 cut(s)	409, 2443
BsaBI	GATNNNNATC	1 cut(s)	975
BsaWI	WCCGGW	1 cut(s)	2548
BsaXI	ACNNNNNCTCC	4 cut(s)	243, 273, 2908, 2938
Bsc4I	CCNNNNNNNGG	4 cut(s)	833, 1179, 2548, 2731
Bse1I	ACTGG	8 cut(s)	151, 818, 980, 1526, 1693, 2013, 2408, 2665
Bse8I	GATNNNNATC	1 cut(s)	975
BseAI	TCCGGA	1 cut(s)	2548
BseBI	CCWGG	7 cut(s)	167, 424, 570, 760, 1180, 1263, 2726
BseJI	GATNNNNATC	1 cut(s)	975
BseLI	CCNNNNNNNGG	4 cut(s)	833, 1179, 2548, 2731
BseMII	CTCAG	4 cut(s)	186, 264, 1674, 2150
BseNI	ACTGG	8 cut(s)	151, 818, 980, 1526, 1693, 2013, 2408, 2665
BseRI	GAGGAG	2 cut(s)	1586, 1862
BseXI	GCAGC	8 cut(s)	250, 253, 1086, 1594, 1807, 1972, 2237, 2363
BseYI	CCCAGC	2 cut(s)	427, 1799
BsgI	GTGCAG	3 cut(s)	1524, 1971, 2370
Bsh1236I	CGCG	1 cut(s)	2448
BshFI	GGCC	4 cut(s)	573, 720, 2520, 2875
BshNI	GGYRCC	2 cut(s)	419, 1072
BsiHKAI	GWGCWC	1 cut(s)	1470
BsiHKCI	CYCGRG	4 cut(s)	1469, 1871, 2028, 2088
BsiSI	CCGG	2 cut(s)	282, 2549
BsiWI	CGTACG	1 cut(s)	2443
BslFI	GGGAC	4 cut(s)	740, 2046, 2515, 2549
BslI	CCNNNNNNNGG	4 cut(s)	833, 1179, 2548, 2731
BsmAI	GTCTC	3 cut(s)	1116, 2198, 2865
BsmFI	GGGAC	4 cut(s)	740, 2046, 2515, 2549
BsnI	GGCC	4 cut(s)	573, 720, 2520, 2875
BsoBI	CYCGRG	4 cut(s)	1469, 1871, 2028, 2088
Bsp1286I	GDGCHC	2 cut(s)	1353, 1470
Bsp13I	TCCGGA	1 cut(s)	2548
Bsp1407I	TGTACA	1 cut(s)	1390
Bsp19I	CCATGG	1 cut(s)	966
BspACI	CCGC	3 cut(s)	1047, 2599, 2869
BspANI	GGCC	4 cut(s)	573, 720, 2520, 2875
BspCNI	CTCAG	4 cut(s)	187, 265, 1673, 2149
BspEI	TCCGGA	1 cut(s)	2548
BspFNI	CGCG	1 cut(s)	2448
BspHI	TCATGA	3 cut(s)	64, 1270, 3106
BspLI	GGNNCC	9 cut(s)	343, 421, 481, 756, 1074, 1575, 1961, 2049, 2685
BspMI	ACCTGC	2 cut(s)	742, 2433
BspQI	GCTCTTC	2 cut(s)	2181, 2886
BspT107I	GGYRCC	2 cut(s)	419, 1072
BspTI	CTTAAG	1 cut(s)	2245
BsrGI	TGTACA	1 cut(s)	1390
BsrI	ACTGG	8 cut(s)	151, 818, 980, 1526, 1693, 2013, 2408, 2665
BssSI	CACGAG	1 cut(s)	1352
BssT1I	CCWWGG	6 cut(s)	415, 966, 1371, 1980, 2344, 2880
Bst2BI	CACGAG	1 cut(s)	1352
Bst2UI	CCWGG	7 cut(s)	167, 424, 570, 760, 1180, 1263, 2726
Bst6I	CTCTTC	5 cut(s)	159, 921, 2181, 2528, 2886
BstAFI	CTTAAG	1 cut(s)	2245
BstAPI	GCANNNNNTGC	1 cut(s)	1547
BstAUI	TGTACA	1 cut(s)	1390
BstBAI	YACGTR	2 cut(s)	409, 2443
BstC8I	GCNNGC	7 cut(s)	575, 712, 1230, 1601, 2597, 2767, 2944
BstDEI	CTNAG	7 cut(s)	195, 273, 1660, 2136, 2639, 2745, 3054
BstDSI	CCRYGG	1 cut(s)	966
BstEII	GGTNACC	1 cut(s)	1475
BstFNI	CGCG	1 cut(s)	2448
BstMAI	GTCTC	3 cut(s)	1116, 2198, 2865
BstMWI	GCNNNNNNNGC	5 cut(s)	283, 1348, 1547, 1582, 2948
BstNI	CCWGG	7 cut(s)	167, 424, 570, 760, 1180, 1263, 2726
BstNSI	RCATGY	3 cut(s)	541, 794, 2195
BstPAI	GACNNNNGTC	1 cut(s)	816
BstPI	GGTNACC	1 cut(s)	1475
BstSCI	CCNGG	8 cut(s)	165, 281, 422, 568, 758, 1178, 1261, 2724
BstSFI	CTRYAG	1 cut(s)	855
BstSNI	TACGTA	1 cut(s)	2443
BstUI	CGCG	1 cut(s)	2448
BstV1I	GCAGC	8 cut(s)	250, 253, 1086, 1594, 1807, 1972, 2237, 2363
BstV2I	GAAGAC	1 cut(s)	2685
BstX2I	RGATCY	8 cut(s)	514, 1157, 1423, 1663, 2047, 2323, 2467, 2683
BstXI	CCANNNNNNTGG	1 cut(s)	131
BstYI	RGATCY	8 cut(s)	514, 1157, 1423, 1663, 2047, 2323, 2467, 2683
BsuI	GTATCC	1 cut(s)	964
BsuRI	GGCC	4 cut(s)	573, 720, 2520, 2875
BtgI	CCRYGG	1 cut(s)	966
BtgZI	GCGATG	2 cut(s)	1073, 2961
BtsI	GCAGTG	3 cut(s)	849, 2374, 3016
BveI	ACCTGC	2 cut(s)	742, 2433
Cac8I	GCNNGC	7 cut(s)	575, 712, 1230, 1601, 2597, 2767, 2944
CaiI	CAGNNNCTG	2 cut(s)	986, 1532
CciI	TCATGA	3 cut(s)	64, 1270, 3106
Cfr13I	GGNCC	7 cut(s)	149, 342, 412, 719, 754, 1748, 2332
CseI	GACGC	1 cut(s)	1351
CsiI	ACCWGGT	1 cut(s)	165
Csp6I	GTAC	6 cut(s)	420, 657, 1391, 2444, 2554, 2735
CspCI	CAANNNNNGTGG	2 cut(s)	64, 99
CviQI	GTAC	6 cut(s)	420, 657, 1391, 2444, 2554, 2735
DdeI	CTNAG	7 cut(s)	195, 273, 1660, 2136, 2639, 2745, 3054
DrdI	GACNNNNNNGTC	1 cut(s)	2857
DseDI	GACNNNNNNGTC	1 cut(s)	2857
Eam1104I	CTCTTC	5 cut(s)	159, 921, 2181, 2528, 2886
EarI	CTCTTC	5 cut(s)	159, 921, 2181, 2528, 2886
EciI	GGCGGA	1 cut(s)	2614
Ecl136II	GAGCTC	1 cut(s)	1468
Eco105I	TACGTA	1 cut(s)	2443
Eco130I	CCWWGG	6 cut(s)	415, 966, 1371, 1980, 2344, 2880
Eco147I	AGGCCT	1 cut(s)	2875
Eco24I	GRGCYC	2 cut(s)	1353, 1470
Eco47I	GGWCC	6 cut(s)	149, 342, 412, 754, 1748, 2332
Eco53kI	GAGCTC	1 cut(s)	1468
Eco57I	CTGAAG	6 cut(s)	1440, 1715, 1944, 2274, 2690, 2814
Eco88I	CYCGRG	4 cut(s)	1469, 1871, 2028, 2088
Eco91I	GGTNACC	1 cut(s)	1475
EcoICRI	GAGCTC	1 cut(s)	1468
EcoO109I	RGGNCCY	1 cut(s)	754
EcoO65I	GGTNACC	1 cut(s)	1475
EcoRI	GAATTC	2 cut(s)	610, 1918
EcoRII	CCWGG	7 cut(s)	165, 422, 568, 758, 1178, 1261, 2724
EcoT14I	CCWWGG	6 cut(s)	415, 966, 1371, 1980, 2344, 2880
EcoT22I	ATGCAT	1 cut(s)	2953
EcoT38I	GRGCYC	2 cut(s)	1353, 1470
ErhI	CCWWGG	6 cut(s)	415, 966, 1371, 1980, 2344, 2880
FalI	AAGNNNNNCTT	6 cut(s)	1009, 1041, 2622, 2654, 3059, 3091
FaqI	GGGAC	4 cut(s)	740, 2046, 2515, 2549
FbaI	TGATCA	1 cut(s)	1267
Fnu4HI	GCNGC	8 cut(s)	264, 267, 1100, 1583, 1821, 1986, 2226, 2352
FriOI	GRGCYC	2 cut(s)	1353, 1470
Fsp4HI	GCNGC	8 cut(s)	264, 267, 1100, 1583, 1821, 1986, 2226, 2352
FspBI	CTAG	5 cut(s)	938, 1428, 1824, 2697, 2714
GluI	GCNGC	8 cut(s)	264, 267, 1100, 1583, 1821, 1986, 2226, 2352
GsaI	CCCAGC	2 cut(s)	431, 1803
GsuI	CTGGAG	1 cut(s)	2708
HaeIII	GGCC	4 cut(s)	573, 720, 2520, 2875
HapII	CCGG	2 cut(s)	282, 2549
HgaI	GACGC	1 cut(s)	1351
HincII	GTYRAC	1 cut(s)	201
HindII	GTYRAC	1 cut(s)	201
HindIII	AAGCTT	1 cut(s)	1626
HpaII	CCGG	2 cut(s)	282, 2549
Hpy166II	GTNNAC	5 cut(s)	201, 393, 935, 1106, 1391
Hpy8I	GTNNAC	5 cut(s)	201, 393, 935, 1106, 1391
HpyCH4IV	ACGT	3 cut(s)	379, 408, 2442
HpyF10VI	GCNNNNNNNGC	5 cut(s)	283, 1348, 1547, 1582, 2948
HpyF3I	CTNAG	7 cut(s)	195, 273, 1660, 2136, 2639, 2745, 3054
HpySE526I	ACGT	3 cut(s)	379, 408, 2442
Kpn2I	TCCGGA	1 cut(s)	2548
KpnI	GGTACC	1 cut(s)	423
Ksp22I	TGATCA	1 cut(s)	1267
LguI	GCTCTTC	2 cut(s)	2181, 2886
LmnI	GCTCC	5 cut(s)	337, 1375, 1573, 1803, 3095
Lsp1109I	GCAGC	8 cut(s)	250, 253, 1086, 1594, 1807, 1972, 2237, 2363
MabI	ACCWGGT	1 cut(s)	165
MaeI	CTAG	5 cut(s)	938, 1428, 1824, 2697, 2714
MaeII	ACGT	3 cut(s)	379, 408, 2442
MaeIII	GTNAC	8 cut(s)	113, 457, 661, 670, 1063, 1475, 1714, 1999
MfeI	CAATTG	1 cut(s)	1794
MflI	RGATCY	8 cut(s)	514, 1157, 1423, 1663, 2047, 2323, 2467, 2683
MhlI	GDGCHC	2 cut(s)	1353, 1470
MluI	ACGCGT	1 cut(s)	2446
MlyI	GAGTC	1 cut(s)	206
MmeI	TCCRAC	2 cut(s)	1726, 3073
Mph1103I	ATGCAT	1 cut(s)	2953
MroI	TCCGGA	1 cut(s)	2548
MroXI	GAANNNNTTC	2 cut(s)	2103, 2831
MseI	TTAA	9 cut(s)	221, 374, 519, 1116, 1244, 1565, 2246, 2648, 3114
MslI	CAYNNNNRTG	5 cut(s)	54, 69, 1070, 2181, 2589
MspA1I	CMGCKG	2 cut(s)	1047, 2792
MspCI	CTTAAG	1 cut(s)	2245
MspI	CCGG	2 cut(s)	282, 2549
MspR9I	CCNGG	8 cut(s)	167, 283, 424, 570, 760, 1180, 1263, 2726
MunI	CAATTG	1 cut(s)	1794
MvaI	CCWGG	7 cut(s)	167, 424, 570, 760, 1180, 1263, 2726
MvnI	CGCG	1 cut(s)	2448
MwoI	GCNNNNNNNGC	5 cut(s)	283, 1348, 1547, 1582, 2948
NciI	CCSGG	1 cut(s)	283
NcoI	CCATGG	1 cut(s)	966
NlaIV	GGNNCC	9 cut(s)	343, 421, 481, 756, 1074, 1575, 1961, 2049, 2685
NmuCI	GTSAC	4 cut(s)	113, 457, 1063, 1475
NsiI	ATGCAT	1 cut(s)	2953
NspI	RCATGY	3 cut(s)	541, 794, 2195
OliI	CACNNNNGTG	2 cut(s)	54, 1070
PaeR7I	CTCGAG	2 cut(s)	1469, 1871
PagI	TCATGA	3 cut(s)	64, 1270, 3106
PceI	AGGCCT	1 cut(s)	2875
PciSI	GCTCTTC	2 cut(s)	2181, 2886
PcsI	WCGNNNNNNNCGW	2 cut(s)	405, 1359
PdmI	GAANNNNTTC	2 cut(s)	2103, 2831
PfeI	GAWTC	9 cut(s)	61, 240, 725, 893, 920, 944, 1034, 2126, 2392
Pfl23II	CGTACG	1 cut(s)	2443
PflMI	CCANNNNNTGG	1 cut(s)	2731
PkrI	GCNGC	8 cut(s)	265, 268, 1101, 1584, 1822, 1987, 2227, 2353
PleI	GAGTC	1 cut(s)	205
PpsI	GAGTC	1 cut(s)	205
Ppu21I	YACGTR	2 cut(s)	409, 2443
PpuMI	RGGWCCY	1 cut(s)	754
PshAI	GACNNNNGTC	1 cut(s)	816
Psp124BI	GAGCTC	1 cut(s)	1470
Psp5II	RGGWCCY	1 cut(s)	754
Psp6I	CCWGG	7 cut(s)	165, 422, 568, 758, 1178, 1261, 2724
PspEI	GGTNACC	1 cut(s)	1475
PspFI	CCCAGC	2 cut(s)	427, 1799
PspGI	CCWGG	7 cut(s)	165, 422, 568, 758, 1178, 1261, 2724
PspLI	CGTACG	1 cut(s)	2443
PspN4I	GGNNCC	9 cut(s)	343, 421, 481, 756, 1074, 1575, 1961, 2049, 2685
PspPI	GGNCC	7 cut(s)	149, 342, 412, 719, 754, 1748, 2332
PspPPI	RGGWCCY	1 cut(s)	754
PspXI	VCTCGAGB	1 cut(s)	1469
PstNI	CAGNNNCTG	2 cut(s)	986, 1532
PsuI	RGATCY	8 cut(s)	514, 1157, 1423, 1663, 2047, 2323, 2467, 2683
PvuII	CAGCTG	1 cut(s)	2792
RsaI	GTAC	6 cut(s)	421, 658, 1392, 2445, 2555, 2736
RsaNI	GTAC	6 cut(s)	420, 657, 1391, 2444, 2554, 2735
RseI	CAYNNNNRTG	5 cut(s)	54, 69, 1070, 2181, 2589
SacI	GAGCTC	1 cut(s)	1470
SapI	GCTCTTC	2 cut(s)	2181, 2886
SaqAI	TTAA	9 cut(s)	221, 374, 519, 1116, 1244, 1565, 2246, 2648, 3114
SatI	GCNGC	8 cut(s)	264, 267, 1100, 1583, 1821, 1986, 2226, 2352
Sau96I	GGNCC	7 cut(s)	149, 342, 412, 719, 754, 1748, 2332
SchI	GAGTC	1 cut(s)	206
ScrFI	CCNGG	8 cut(s)	167, 283, 424, 570, 760, 1180, 1263, 2726
SduI	GDGCHC	2 cut(s)	1353, 1470
SexAI	ACCWGGT	1 cut(s)	165
SfcI	CTRYAG	1 cut(s)	855
Sfr274I	CTCGAG	2 cut(s)	1469, 1871
SinI	GGWCC	6 cut(s)	149, 342, 412, 754, 1748, 2332
SlaI	CTCGAG	2 cut(s)	1469, 1871
SmiMI	CAYNNNNRTG	5 cut(s)	54, 69, 1070, 2181, 2589
SmlI	CTYRAG	6 cut(s)	1469, 1752, 1871, 2245, 2473, 2936
SmoI	CTYRAG	6 cut(s)	1469, 1752, 1871, 2245, 2473, 2936
SnaBI	TACGTA	1 cut(s)	2443
SseBI	AGGCCT	1 cut(s)	2875
SsiI	CCGC	3 cut(s)	1047, 2599, 2869
SspI	AATATT	1 cut(s)	2217
SspMI	CTAG	5 cut(s)	938, 1428, 1824, 2697, 2714
SstI	GAGCTC	1 cut(s)	1470
StuI	AGGCCT	1 cut(s)	2875
StyD4I	CCNGG	8 cut(s)	165, 281, 422, 568, 758, 1178, 1261, 2724
StyI	CCWWGG	6 cut(s)	415, 966, 1371, 1980, 2344, 2880
TaiI	ACGT	3 cut(s)	382, 411, 2445
TaqI	TCGA	7 cut(s)	172, 235, 1470, 1774, 1872, 2461, 3023
TatI	WGTACW	1 cut(s)	1390
TfiI	GAWTC	9 cut(s)	61, 240, 725, 893, 920, 944, 1034, 2126, 2392
Tru1I	TTAA	9 cut(s)	221, 374, 519, 1116, 1244, 1565, 2246, 2648, 3114
Tru9I	TTAA	9 cut(s)	221, 374, 519, 1116, 1244, 1565, 2246, 2648, 3114
TseFI	GTSAC	4 cut(s)	113, 457, 1063, 1475
TseI	GCWGC	8 cut(s)	263, 266, 1099, 1582, 1820, 1985, 2225, 2351
Tsp45I	GTSAC	4 cut(s)	113, 457, 1063, 1475
TspGWI	ACGGA	4 cut(s)	589, 966, 1237, 2559
Van91I	CCANNNNNTGG	1 cut(s)	2731
Vha464I	CTTAAG	1 cut(s)	2245
VpaK11BI	GGWCC	6 cut(s)	149, 342, 412, 754, 1748, 2332
XceI	RCATGY	3 cut(s)	541, 794, 2195
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2342
XhoI	CTCGAG	2 cut(s)	1469, 1871
XmnI	GAANNNNTTC	2 cut(s)	2103, 2831
XspI	CTAG	5 cut(s)	938, 1428, 1824, 2697, 2714
Zsp2I	ATGCAT	1 cut(s)	2953

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.