Rh6AG226500

Belongs to the ubiquitin-conjugating enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0005883
Physical Location & Seq
Reverse (-)
40800767 .. 40816076
15310 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG226500.1

Sequence Viewer

Length: 3594 bp
ATGATCTTGACTTGGTCGGCGGAGGGGGATGTCAAGGGGCTCACACCAATAGACGGCGTTCTCGTTGCCGGCTCAGCCGTGGAAGACGGGGAGGTTCTCGAGGGCAATGAGCTTGTGGAGATGAGACTGAGGTCTGGAGCTCGGTTTGCTTGGTGGAGATGGAGAGTGTGGAGATTTGAAGCTCATGGATCTGAGATTCCAATTGCTCTATGGAGCTCTTCGATTTTGCTTCACCAGCGTGACGAATATACCCCCCTTGTAGCTCCTCCGCCCTCTCCTGCATTATATTACAAGACAAACCAAAACACCTCTGCGACTTCCCTCCCTCTCAAAGAACCAAGCTTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCCTTCACCTACCGCACCATCTCCATCTCCATCTCTTCCAACTCCTTCAGATTTTGGTATGGGAAGCAAGCAGCATGACAGTGTTTTTAAAGACGATGAACCTACTACTGTAGACCATGATGATAGTTCTTTGATCCAGGTTGAGTTTTCAACTGATTCAAATGTGAATAATGAATTTAAAAAGTTGAGTGAGGTTGTCGGTGGCATGAATAATGCTCCATACATATATAGACAAGATGTTGTGAGGAGCAAGAATGGTATGATTGGGGTTGTGACTGAAGTTGCTGGTGATTTGGATTCTGATAGTGGCATGTCTGATGATGAGGATGAGGATGAGGATGAAATAGATGACGACGATGACGACGACCACAACAGTGAGAATCATGAAAATGACAAAGATGGTGGTGATGACAGTAATACTAATGAAAATGGTGACAAGAACCAGAATGGTGGTAATGATAAGACTGGTCCTCTTCCTGCAGACCAGGTTCGAGTGCTTTGGATAGATGAAACTGAATCAACCCAGAATATCAATGATATAACAGTTGTTGATCGAGGATTCTTGCATGGAGATTTTGTTGCTGCTGCTTCTGAGCCAACCGGGCAAGTTGGTGTCGTGGTGGATGTCAATATCTCTGTTGATCTGAAAGCTCCTGACGGGTCCATCTTAAAAGATGTGTCAGCCAATGACTTAAAACGTGTGAGGGAGTTCACTGTTGGTGATTATGTGGTCCTTGGTACCTGGCTGGGTAGAATTGAGGATGTTTTTGATAATGTGACGGTGATGATTGATGATGGTTCCCTATGTAGAATTTTGAGAGCAGATCCGATGGATCTTAAACCACTTTCCAAAAATATGCTTGAAGATGTGCATTTCCCTTATTACCCAGGCCAGCGTGTGAAGGCAAGGTCATCATCGGTTTTTAAGAATTCTTTGTGGCTATCCGGCTCATGGAAGGCTAATCGTCTGGAAGGTACTGTTACTAAAGTTACAGTGGGATCAGTGTTTATTTATTGGATAGCTTCTGCTGGCTGTGGCCCAGATTCTTCAACTGCCCCTGCTAAAGAGCAGGTCCCCAGGAAGTTGAAACTATTGTCTTGTTTCACACATGCAAATTGGCAGTTGGGCGACTGGTGTCTCTTTCCATCATCAGGGTCATCATCTTCCACTGCCGTAGATAAGGGTTCAAAATTAGAACTTCATCAATTAGATTCTACTCAAATGGGTAATGTGTGTGATTCGGAAGAGAGTTCACTAGAAGAACCAAATGGAAATAGTGCATCCATGGATACTGACCCGGTGTCTGTATTAGATGGAAACAATGGAAATGCTGGAAGTAATACTTCTATTGAATCTAGTTCCAGCGGTAGTTCATTGTCAGTCACAAAGGTGCCTGTTTCTCTTCATCGCAAAAAGCTGCGTAAACCTGTGGTTAAAAGAGACAAGAAGGCACGAAAGGAGGAAAATTTTGAAAGATCTTTTTTGATTGCCAATACCAGGACAACAGTTGATGTGGCATGGCAGGATGGTTCAACAGAACGGAAGCTGGCTTCAACGAATTTAATTCCTCTTGATAGTCCTGGTGATCATGAATTTGTTGCTGAACAATATGTGGTGGAGAAGGCATCTGATGATGGTGACAATGCTTTTGAAGCTAGGCGTGTTGGACTCGTGAAAAGCGTCAATGCCAAGGAGCGAACAGCTTGTGTACAGTGGTTGAAGGCAGTTTCCAGAGCTGAAGATCCTAGAGAGTTTGACAAGGAGGAAGTTGTTAGTGTGTATGAGCTTGAGGGTCACCCAGATTATGATTACTGTTATGGGGATGTTGTTGTCAGATTGTTGCCAGTTTCTGTTTCTGCACAAACTGCTTCGGGATCGGACTTAATTGAGGAGCCAAAGCAGCAAAATCAACCAAGTGAGCTTACATCAGAAGTTGTTGATGAAGCTTCTTCTGTTGATAAATCTTGTATGGACTTCTCAGATCTTTCTTGGGTTGGGAATATAACTGGTCTTAAGAATGGTGATATTGAAGTTACATGGGCTGACGGGATGGTTTCTACGGTTGGACCTCAAGCAATTTATGTTGTTGGTCGAGCTGATGATGATGACTCAATTGCTGGGAGCGAGGTTAGTGATGCTGCTAGTTGGGAAACAGTTGATGATGAGGAGGTGCATGCCCTATTTCCCCTCGAGAGTATAACAGAGGAAGTGGAGATACAGAGTGCCTTTAACATGAATTCTGAAGCAGAGGTGGGTGGAGAAGATAATTCTGGAAGGAACCCTGCATTGTCTGTTCCCTTGGCTGCATTTCGCTTTGTTACCAGACTGGCAAGTGGAATATTTGCTCGGGGACAAAAGAACTTGGATCCAATTTTAGATACTAAAGGTGAAGGTGATTTTGAGCCTCGGGAAGTTGAAATCTTTCAGGGAGATCATTGTGAAGATTCCAGCTCTCAGAAATCTAAAGTTGTTGATATTAGTGTGGAGACCAATCATAAAAATGAAGAGCATGTTTCCTTGGAGACAACAAAAATATTGGATGCAGCAGAAATCTTGTGCAACTTAAGGACTGAAGGATTGGATGCTAAAGAATGTAGCAAGGGTGATGTTTGCAGTTTCAAACGCTTTGATATAGTCAAAGATCCTTTGGACCATCATTATCTTGGTGCAGCAGGGCAGGATAGCGGTGGTAAGAAGTGGTTCAAGAAGATTCAGCAAGATTGGGGTATCCTTCAAAATAACCTGCCCGATGGCATCTACGTACGCGTTTATGAAGATCGAATGGATCTCTTGAGGGCAGTTATAGTTGGGGCTTATGGGACACCTTACCAAGATGGCCTCTTCTTTTTCGATTTTCATCTCCCTCCGGAGTACCCTGATGTCCCTCCGTCAGCATATTATCATTCTGGTGGCTGGCGGATAAATCCAAATCTATACGAAGAAGGCAAAGTCTGTCTTAGTCTGTTAAATACTTGGACTGGTAGAGGAAATGAAGTTTGGGATCCAAAGTCTTCTAGCATCCTTCAAGTTCTAGTTTCACTCCAGGGATTGGTGCTAAATTCTAAGCCCTATTTCAACGAAGCTGGCTATGATAAGCAAATTGGAACAGCTGAAGGGGAGAAAAATTCGTTGTCCTACAATGAGAATACTTTCTTGCTGAATTGCAAGACGATAATGTCTCTTATGCGGAGGCCTCCCAAGGTGATGATCATGTTATAA

Protein Analysis

1197

Amino Acids

131.57

Weight (kDa)

4.53

Isoelectric Point (pI)

37.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SH3-A_UBE2O PF23048 200 - 308 6.8e-06 UBE2O, N-terminal SH3-A domain
tSH3-B_UBE2O PF23046 364 - 495 1.8e-42 UBE2O, tandem tSH3-B domain
SH3-B_UBE2O PF23043 654 - 726 3.8e-20 UBE2O, SH3-B domain
SH3-C_UBE2O PF23044 785 - 827 1.7e-06 UBE2O, ubiquitin binding SH3-C domain
UQ_con PF00179 1029 - 1161 9.1e-20 Ubiquitin-conjugating enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3592
AasI GACNNNNNNGTC 1 cut(s) 3550
Acc36I ACCTGC 2 cut(s) 1435, 3126
Acc65I GGTACC 1 cut(s) 1112
AccB1I GGYRCC 2 cut(s) 1112, 1765
AccB7I CCANNNNNTGG 1 cut(s) 3424
AccI GTMKAC 1 cut(s) 486
AccII CGCG 1 cut(s) 3141
AccIII TCCGGA 1 cut(s) 3241
AciI CCGC 7 cut(s) 20, 269, 387, 1740, 3060, 3292, 3562
AcsI RAATTY 9 cut(s) 548, 1185, 1303, 1840, 1933, 1967, 2611, 3433, 3499
AcuI CTGAAG 6 cut(s) 405, 672, 2133, 2637, 2967, 3507
AfaI GTAC 5 cut(s) 1114, 1351, 2085, 3138, 3248
AfiI CCNNNNNNNGG 5 cut(s) 53, 1326, 1526, 1872, 3424
AflII CTTAAG 2 cut(s) 2387, 2938
AflIII ACRYGT 2 cut(s) 1072, 3139
AhdI GACNNNNNGTC 1 cut(s) 1675
AjnI CCWGG 8 cut(s) 510, 858, 1115, 1261, 1451, 1871, 1954, 3417
AjuI GAANNNNNNNTTGG 2 cut(s) 3355, 3387
AleI CACNNNNGTG 3 cut(s) 237, 747, 1763
Alw21I GWGCWC 2 cut(s) 142, 218
Alw26I GTCTC 6 cut(s) 118, 1517, 1809, 2855, 2891, 3558
AlwNI CAGNNNCTG 1 cut(s) 2225
Ama87I CYCGRG 4 cut(s) 98, 2564, 2721, 2781
Aor13HI TCCGGA 1 cut(s) 3241
AoxI GGCC 4 cut(s) 1264, 1411, 3211, 3566
ApeKI GCWGC 9 cut(s) 445, 956, 959, 1792, 2275, 2513, 2678, 2918, 3044
ApoI RAATTY 9 cut(s) 548, 1185, 1303, 1840, 1933, 1967, 2611, 3433, 3499
ArsI GACNNNNNNTTYG 2 cut(s) 2006, 2038
Asp700I GAANNNNTTC 3 cut(s) 2796, 3074, 3524
Asp718I GGTACC 1 cut(s) 1112
AspS9I GGNCC 7 cut(s) 842, 1035, 1105, 1412, 1447, 2441, 3025
AsuC2I CCSGG 2 cut(s) 976, 1673
AvaI CYCGRG 4 cut(s) 98, 2564, 2721, 2781
AvaII GGWCC 6 cut(s) 842, 1035, 1105, 1447, 2441, 3025
BamHI GGATCC 2 cut(s) 2740, 3376
BanI GGYRCC 2 cut(s) 1112, 1765
BanII GRGCYC 3 cut(s) 42, 142, 218
BarI GAAGNNNNNNTAC 2 cut(s) 2574, 2606
BauI CACGAG 1 cut(s) 2045
BbsI GAAGAC 2 cut(s) 90, 3378
Bbv12I GWGCWC 2 cut(s) 142, 218
BbvI GCAGC 9 cut(s) 457, 943, 946, 1779, 2287, 2500, 2665, 2930, 3056
BceAI ACGGC 3 cut(s) 62, 70, 1532
BciT130I CCWGG 8 cut(s) 512, 860, 1117, 1263, 1453, 1873, 1956, 3419
BciVI GTATCC 2 cut(s) 1657, 3113
BclI TGATCA 2 cut(s) 1960, 3582
BcnI CCSGG 2 cut(s) 976, 1673
BcoDI GTCTC 6 cut(s) 118, 1517, 1809, 2855, 2891, 3558
BfaI CTAG 7 cut(s) 1631, 1731, 2031, 2121, 2517, 3390, 3407
BfmI CTRYAG 2 cut(s) 483, 852
BfrI CTTAAG 2 cut(s) 2387, 2938
BfuAI ACCTGC 2 cut(s) 1435, 3126
BfuI GTATCC 2 cut(s) 1657, 3113
BglI GCCNNNNNGGC 1 cut(s) 976
BglII AGATCT 2 cut(s) 1850, 2356
BisI GCNGC 9 cut(s) 446, 957, 960, 1793, 2276, 2514, 2679, 2919, 3045
BlpI GCTNAGC 1 cut(s) 73
BlsI GCNGC 9 cut(s) 447, 958, 961, 1794, 2277, 2515, 2680, 2920, 3046
Bme18I GGWCC 6 cut(s) 842, 1035, 1105, 1447, 2441, 3025
BmeRI GACNNNNNGTC 1 cut(s) 1675
BmeT110I CYCGRG 4 cut(s) 98, 2564, 2721, 2781
BmgT120I GGNCC 7 cut(s) 842, 1035, 1105, 1412, 1447, 2441, 3025
BmiI GGNNCC 9 cut(s) 1036, 1114, 1174, 1449, 1767, 2268, 2654, 2742, 3378
BmsI GCATC 7 cut(s) 1664, 2009, 2500, 2905, 2947, 3138, 3402
BoxI GACNNNNGTC 2 cut(s) 130, 1509
BpiI GAAGAC 2 cut(s) 90, 3378
BpmI CTGGAG 2 cut(s) 156, 3401
Bpu1102I GCTNAGC 1 cut(s) 73
BpuEI CTTGAG 3 cut(s) 2183, 2430, 3187
BpuMI CCSGG 2 cut(s) 976, 1673
BsaAI YACGTR 1 cut(s) 3136
BsaBI GATNNNNATC 1 cut(s) 3231
BsaI GGTCTC 1 cut(s) 2855
BsaWI WCCGGW 1 cut(s) 3241
BsaXI ACNNNNNCTCC 2 cut(s) 936, 966
Bsc4I CCNNNNNNNGG 5 cut(s) 53, 1326, 1526, 1872, 3424
Bse118I RCCGGY 1 cut(s) 68
Bse1I ACTGG 6 cut(s) 844, 1511, 2219, 2386, 2706, 3358
Bse3DI GCAATG 1 cut(s) 112
Bse8I GATNNNNATC 1 cut(s) 3231
BseAI TCCGGA 1 cut(s) 3241
BseBI CCWGG 8 cut(s) 512, 860, 1117, 1263, 1453, 1873, 1956, 3419
BseJI GATNNNNATC 1 cut(s) 3231
BseLI CCNNNNNNNGG 5 cut(s) 53, 1326, 1526, 1872, 3424
BseMI GCAATG 1 cut(s) 112
BseMII CTCAG 6 cut(s) 87, 119, 183, 957, 2367, 2843
BseNI ACTGG 6 cut(s) 844, 1511, 2219, 2386, 2706, 3358
BseRI GAGGAG 4 cut(s) 255, 634, 2279, 2555
BseXI GCAGC 9 cut(s) 457, 943, 946, 1779, 2287, 2500, 2665, 2930, 3056
BseYI CCCAGC 2 cut(s) 1120, 2492
BsgI GTGCAG 2 cut(s) 2217, 3063
Bsh1236I CGCG 1 cut(s) 3141
BshFI GGCC 4 cut(s) 1266, 1413, 3213, 3568
BshNI GGYRCC 2 cut(s) 1112, 1765
BsiHKAI GWGCWC 2 cut(s) 142, 218
BsiHKCI CYCGRG 4 cut(s) 98, 2564, 2721, 2781
BsiSI CCGG 5 cut(s) 69, 975, 1320, 1673, 3242
BsiWI CGTACG 1 cut(s) 3136
BslFI GGGAC 4 cut(s) 1433, 2739, 3208, 3242
BslI CCNNNNNNNGG 5 cut(s) 53, 1326, 1526, 1872, 3424
BsmAI GTCTC 6 cut(s) 118, 1517, 1809, 2855, 2891, 3558
BsmFI GGGAC 4 cut(s) 1433, 2739, 3208, 3242
BsnI GGCC 4 cut(s) 1266, 1413, 3213, 3568
Bso31I GGTCTC 1 cut(s) 2855
BsoBI CYCGRG 4 cut(s) 98, 2564, 2721, 2781
Bsp1286I GDGCHC 3 cut(s) 42, 142, 218
Bsp13I TCCGGA 1 cut(s) 3241
Bsp1407I TGTACA 1 cut(s) 2083
Bsp1720I GCTNAGC 1 cut(s) 73
Bsp19I CCATGG 1 cut(s) 1659
BspACI CCGC 7 cut(s) 20, 269, 387, 1740, 3060, 3292, 3562
BspANI GGCC 4 cut(s) 1266, 1413, 3213, 3568
BspCNI CTCAG 6 cut(s) 86, 120, 184, 958, 2366, 2842
BspEI TCCGGA 1 cut(s) 3241
BspFNI CGCG 1 cut(s) 3141
BspHI TCATGA 2 cut(s) 757, 1963
BspLI GGNNCC 9 cut(s) 1036, 1114, 1174, 1449, 1767, 2268, 2654, 2742, 3378
BspMAI CTGCAG 1 cut(s) 856
BspMI ACCTGC 2 cut(s) 1435, 3126
BspQI GCTCTTC 2 cut(s) 223, 2874
BspT107I GGYRCC 2 cut(s) 1112, 1765
BspTI CTTAAG 2 cut(s) 2387, 2938
BspTNI GGTCTC 1 cut(s) 2855
BsrDI GCAATG 1 cut(s) 112
BsrFI RCCGGY 1 cut(s) 68
BsrGI TGTACA 1 cut(s) 2083
BsrI ACTGG 6 cut(s) 844, 1511, 2219, 2386, 2706, 3358
BssAI RCCGGY 1 cut(s) 68
BssSI CACGAG 1 cut(s) 2045
BssT1I CCWWGG 6 cut(s) 1108, 1659, 2064, 2673, 2892, 3573
Bst2BI CACGAG 1 cut(s) 2045
Bst2UI CCWGG 8 cut(s) 512, 860, 1117, 1263, 1453, 1873, 1956, 3419
Bst6I CTCTTC 7 cut(s) 223, 414, 852, 1614, 1782, 2874, 3221
BstAFI CTTAAG 2 cut(s) 2387, 2938
BstAPI GCANNNNNTGC 1 cut(s) 2240
BstAUI TGTACA 1 cut(s) 2083
BstBAI YACGTR 1 cut(s) 3136
BstC8I GCNNGC 8 cut(s) 70, 443, 1268, 1405, 1923, 2550, 3290, 3460
BstDEI CTNAG 8 cut(s) 73, 128, 192, 966, 2353, 2829, 3332, 3438
BstDSI CCRYGG 2 cut(s) 78, 1659
BstEII GGTNACC 1 cut(s) 2168
BstFNI CGCG 1 cut(s) 3141
BstMAI GTCTC 6 cut(s) 118, 1517, 1809, 2855, 2891, 3558
BstMWI GCNNNNNNNGC 7 cut(s) 74, 146, 235, 976, 2027, 2240, 2275
BstNI CCWGG 8 cut(s) 512, 860, 1117, 1263, 1453, 1873, 1956, 3419
BstNSI RCATGY 4 cut(s) 688, 1487, 2552, 2888
BstPAI GACNNNNGTC 2 cut(s) 130, 1509
BstPI GGTNACC 1 cut(s) 2168
BstSFI CTRYAG 2 cut(s) 483, 852
BstSNI TACGTA 1 cut(s) 3136
BstUI CGCG 1 cut(s) 3141
BstV1I GCAGC 9 cut(s) 457, 943, 946, 1779, 2287, 2500, 2665, 2930, 3056
BstV2I GAAGAC 2 cut(s) 90, 3378
BstXI CCANNNNNNTGG 1 cut(s) 824
BsuI GTATCC 2 cut(s) 1657, 3113
BsuRI GGCC 4 cut(s) 1266, 1413, 3213, 3568
BtgI CCRYGG 2 cut(s) 78, 1659
BtgZI GCGATG 1 cut(s) 1766
BtsI GCAGTG 1 cut(s) 1542
BtsIMutI CAGTG 7 cut(s) 460, 754, 1086, 1374, 1383, 1542, 2093
BveI ACCTGC 2 cut(s) 1435, 3126
Cac8I GCNNGC 8 cut(s) 70, 443, 1268, 1405, 1923, 2550, 3290, 3460
CaiI CAGNNNCTG 1 cut(s) 2225
CciI TCATGA 2 cut(s) 757, 1963
Cfr10I RCCGGY 1 cut(s) 68
Cfr13I GGNCC 7 cut(s) 842, 1035, 1105, 1412, 1447, 2441, 3025
CseI GACGC 1 cut(s) 2044
CsiI ACCWGGT 1 cut(s) 858
Csp6I GTAC 5 cut(s) 1113, 1350, 2084, 3137, 3247
CspCI CAANNNNNGTGG 2 cut(s) 757, 792
CviQI GTAC 5 cut(s) 1113, 1350, 2084, 3137, 3247
DdeI CTNAG 8 cut(s) 73, 128, 192, 966, 2353, 2829, 3332, 3438
DraI TTTAAA 2 cut(s) 463, 553
DrdI GACNNNNNNGTC 1 cut(s) 3550
DriI GACNNNNNGTC 1 cut(s) 1675
DseDI GACNNNNNNGTC 1 cut(s) 3550
Eam1104I CTCTTC 7 cut(s) 223, 414, 852, 1614, 1782, 2874, 3221
Eam1105I GACNNNNNGTC 1 cut(s) 1675
EarI CTCTTC 7 cut(s) 223, 414, 852, 1614, 1782, 2874, 3221
EciI GGCGGA 3 cut(s) 35, 258, 3307
Ecl136II GAGCTC 2 cut(s) 140, 216
Eco105I TACGTA 1 cut(s) 3136
Eco130I CCWWGG 6 cut(s) 1108, 1659, 2064, 2673, 2892, 3573
Eco147I AGGCCT 1 cut(s) 3568
Eco24I GRGCYC 3 cut(s) 42, 142, 218
Eco31I GGTCTC 1 cut(s) 2855
Eco47I GGWCC 6 cut(s) 842, 1035, 1105, 1447, 2441, 3025
Eco53kI GAGCTC 2 cut(s) 140, 216
Eco57I CTGAAG 6 cut(s) 405, 672, 2133, 2637, 2967, 3507
Eco88I CYCGRG 4 cut(s) 98, 2564, 2721, 2781
Eco91I GGTNACC 1 cut(s) 2168
EcoICRI GAGCTC 2 cut(s) 140, 216
EcoO109I RGGNCCY 1 cut(s) 1447
EcoO65I GGTNACC 1 cut(s) 2168
EcoRI GAATTC 2 cut(s) 1303, 2611
EcoRII CCWGG 8 cut(s) 510, 858, 1115, 1261, 1451, 1871, 1954, 3417
EcoT14I CCWWGG 6 cut(s) 1108, 1659, 2064, 2673, 2892, 3573
EcoT38I GRGCYC 3 cut(s) 42, 142, 218
ErhI CCWWGG 6 cut(s) 1108, 1659, 2064, 2673, 2892, 3573
FalI AAGNNNNNCTT 4 cut(s) 1702, 1734, 3315, 3347
FaqI GGGAC 4 cut(s) 1433, 2739, 3208, 3242
FbaI TGATCA 2 cut(s) 1960, 3582
FblI GTMKAC 1 cut(s) 486
Fnu4HI GCNGC 9 cut(s) 446, 957, 960, 1793, 2276, 2514, 2679, 2919, 3045
FriOI GRGCYC 3 cut(s) 42, 142, 218
Fsp4HI GCNGC 9 cut(s) 446, 957, 960, 1793, 2276, 2514, 2679, 2919, 3045
FspBI CTAG 7 cut(s) 1631, 1731, 2031, 2121, 2517, 3390, 3407
GluI GCNGC 9 cut(s) 446, 957, 960, 1793, 2276, 2514, 2679, 2919, 3045
GsaI CCCAGC 2 cut(s) 1124, 2496
GsuI CTGGAG 2 cut(s) 156, 3401
HaeIII GGCC 4 cut(s) 1266, 1413, 3213, 3568
HapII CCGG 5 cut(s) 69, 975, 1320, 1673, 3242
HgaI GACGC 1 cut(s) 2044
HindIII AAGCTT 2 cut(s) 340, 2319
HpaII CCGG 5 cut(s) 69, 975, 1320, 1673, 3242
Hpy166II GTNNAC 5 cut(s) 487, 1086, 1628, 1799, 2084
Hpy8I GTNNAC 5 cut(s) 487, 1086, 1628, 1799, 2084
Hpy99I CGWCG 2 cut(s) 731, 740
HpyCH4IV ACGT 2 cut(s) 1072, 3135
HpyF10VI GCNNNNNNNGC 7 cut(s) 74, 146, 235, 976, 2027, 2240, 2275
HpyF3I CTNAG 8 cut(s) 73, 128, 192, 966, 2353, 2829, 3332, 3438
HpySE526I ACGT 2 cut(s) 1072, 3135
Kpn2I TCCGGA 1 cut(s) 3241
KpnI GGTACC 1 cut(s) 1116
KroI GCCGGC 1 cut(s) 68
KroNI GCCGGC 1 cut(s) 70
Ksp22I TGATCA 2 cut(s) 1960, 3582
LguI GCTCTTC 2 cut(s) 223, 2874
LmnI GCTCC 9 cut(s) 137, 213, 268, 595, 621, 1030, 2068, 2266, 2496
Lsp1109I GCAGC 9 cut(s) 457, 943, 946, 1779, 2287, 2500, 2665, 2930, 3056
LweI GCATC 7 cut(s) 1664, 2009, 2500, 2905, 2947, 3138, 3402
MabI ACCWGGT 1 cut(s) 858
MaeI CTAG 7 cut(s) 1631, 1731, 2031, 2121, 2517, 3390, 3407
MaeII ACGT 2 cut(s) 1072, 3135
MfeI CAATTG 2 cut(s) 201, 2487
MhlI GDGCHC 3 cut(s) 42, 142, 218
MluI ACGCGT 1 cut(s) 3139
MlyI GAGTC 2 cut(s) 2037, 2477
MmeI TCCRAC 3 cut(s) 437, 2020, 2419
MroI TCCGGA 1 cut(s) 3241
MroNI GCCGGC 1 cut(s) 68
MroXI GAANNNNTTC 3 cut(s) 2796, 3074, 3524
MslI CAYNNNNRTG 7 cut(s) 237, 453, 747, 762, 1763, 2874, 3282
MspA1I CMGCKG 2 cut(s) 1740, 3485
MspCI CTTAAG 2 cut(s) 2387, 2938
MspI CCGG 5 cut(s) 69, 975, 1320, 1673, 3242
MunI CAATTG 2 cut(s) 201, 2487
MvaI CCWGG 8 cut(s) 512, 860, 1117, 1263, 1453, 1873, 1956, 3419
MvnI CGCG 1 cut(s) 3141
MwoI GCNNNNNNNGC 7 cut(s) 74, 146, 235, 976, 2027, 2240, 2275
NaeI GCCGGC 1 cut(s) 70
NciI CCSGG 2 cut(s) 976, 1673
NcoI CCATGG 1 cut(s) 1659
NgoMIV GCCGGC 1 cut(s) 68
NlaIV GGNNCC 9 cut(s) 1036, 1114, 1174, 1449, 1767, 2268, 2654, 2742, 3378
NmuCI GTSAC 7 cut(s) 239, 646, 806, 1150, 1756, 2012, 2168
NspI RCATGY 4 cut(s) 688, 1487, 2552, 2888
OliI CACNNNNGTG 3 cut(s) 237, 747, 1763
PaeI GCATGC 1 cut(s) 2552
PaeR7I CTCGAG 2 cut(s) 98, 2564
PagI TCATGA 2 cut(s) 757, 1963
PceI AGGCCT 1 cut(s) 3568
PciSI GCTCTTC 2 cut(s) 223, 2874
PcsI WCGNNNNNNNCGW 4 cut(s) 60, 732, 735, 2052
PdiI GCCGGC 1 cut(s) 70
PdmI GAANNNNTTC 3 cut(s) 2796, 3074, 3524
Pfl23II CGTACG 1 cut(s) 3136
PflFI GACNNNGTC 1 cut(s) 13
PflMI CCANNNNNTGG 1 cut(s) 3424
PkrI GCNGC 9 cut(s) 447, 958, 961, 1794, 2277, 2515, 2680, 2920, 3046
PleI GAGTC 2 cut(s) 2037, 2477
PpsI GAGTC 2 cut(s) 2037, 2477
Ppu21I YACGTR 1 cut(s) 3136
PpuMI RGGWCCY 1 cut(s) 1447
PshAI GACNNNNGTC 2 cut(s) 130, 1509
PsiI TTATAA 1 cut(s) 3592
Psp124BI GAGCTC 2 cut(s) 142, 218
Psp5II RGGWCCY 1 cut(s) 1447
Psp6I CCWGG 8 cut(s) 510, 858, 1115, 1261, 1451, 1871, 1954, 3417
PspEI GGTNACC 1 cut(s) 2168
PspFI CCCAGC 2 cut(s) 1120, 2492
PspGI CCWGG 8 cut(s) 510, 858, 1115, 1261, 1451, 1871, 1954, 3417
PspLI CGTACG 1 cut(s) 3136
PspN4I GGNNCC 9 cut(s) 1036, 1114, 1174, 1449, 1767, 2268, 2654, 2742, 3378
PspPI GGNCC 7 cut(s) 842, 1035, 1105, 1412, 1447, 2441, 3025
PspPPI RGGWCCY 1 cut(s) 1447
PstI CTGCAG 1 cut(s) 856
PstNI CAGNNNCTG 1 cut(s) 2225
PsyI GACNNNGTC 1 cut(s) 13
PvuII CAGCTG 1 cut(s) 3485
RsaI GTAC 5 cut(s) 1114, 1351, 2085, 3138, 3248
RsaNI GTAC 5 cut(s) 1113, 1350, 2084, 3137, 3247
RseI CAYNNNNRTG 7 cut(s) 237, 453, 747, 762, 1763, 2874, 3282
SacI GAGCTC 2 cut(s) 142, 218
SapI GCTCTTC 2 cut(s) 223, 2874
SatI GCNGC 9 cut(s) 446, 957, 960, 1793, 2276, 2514, 2679, 2919, 3045
Sau96I GGNCC 7 cut(s) 842, 1035, 1105, 1412, 1447, 2441, 3025
SchI GAGTC 2 cut(s) 2037, 2477
SduI GDGCHC 3 cut(s) 42, 142, 218
SexAI ACCWGGT 1 cut(s) 858
SfaNI GCATC 7 cut(s) 1664, 2009, 2500, 2905, 2947, 3138, 3402
SfcI CTRYAG 2 cut(s) 483, 852
Sfr274I CTCGAG 2 cut(s) 98, 2564
SinI GGWCC 6 cut(s) 842, 1035, 1105, 1447, 2441, 3025
SlaI CTCGAG 2 cut(s) 98, 2564
SmiMI CAYNNNNRTG 7 cut(s) 237, 453, 747, 762, 1763, 2874, 3282
SmlI CTYRAG 7 cut(s) 98, 2162, 2387, 2445, 2564, 2938, 3166
SmoI CTYRAG 7 cut(s) 98, 2162, 2387, 2445, 2564, 2938, 3166
SnaBI TACGTA 1 cut(s) 3136
SphI GCATGC 1 cut(s) 2552
SseBI AGGCCT 1 cut(s) 3568
SsiI CCGC 7 cut(s) 20, 269, 387, 1740, 3060, 3292, 3562
SspI AATATT 2 cut(s) 2715, 2910
SspMI CTAG 7 cut(s) 1631, 1731, 2031, 2121, 2517, 3390, 3407
SstI GAGCTC 2 cut(s) 142, 218
StuI AGGCCT 1 cut(s) 3568
StyI CCWWGG 6 cut(s) 1108, 1659, 2064, 2673, 2892, 3573
TaiI ACGT 2 cut(s) 1075, 3138
TaqI TCGA 8 cut(s) 99, 221, 865, 928, 2467, 2565, 3154, 3225
TatI WGTACW 1 cut(s) 2083
TscAI CASTG 7 cut(s) 460, 754, 1093, 1374, 1383, 1549, 2093
TseFI GTSAC 7 cut(s) 239, 646, 806, 1150, 1756, 2012, 2168
TseI GCWGC 9 cut(s) 445, 956, 959, 1792, 2275, 2513, 2678, 2918, 3044
Tsp45I GTSAC 7 cut(s) 239, 646, 806, 1150, 1756, 2012, 2168
TspGWI ACGGA 2 cut(s) 1930, 3252
TspRI CASTG 7 cut(s) 460, 754, 1093, 1374, 1383, 1549, 2093
Tth111I GACNNNGTC 1 cut(s) 13
Van91I CCANNNNNTGG 1 cut(s) 3424
Vha464I CTTAAG 2 cut(s) 2387, 2938
VpaK11BI GGWCC 6 cut(s) 842, 1035, 1105, 1447, 2441, 3025
XapI RAATTY 9 cut(s) 548, 1185, 1303, 1840, 1933, 1967, 2611, 3433, 3499
XceI RCATGY 4 cut(s) 688, 1487, 2552, 2888
XcmI CCANNNNNNNNNTGG 2 cut(s) 207, 3035
XhoI CTCGAG 2 cut(s) 98, 2564
XmiI GTMKAC 1 cut(s) 486
XmnI GAANNNNTTC 3 cut(s) 2796, 3074, 3524
XspI CTAG 7 cut(s) 1631, 1731, 2031, 2121, 2517, 3390, 3407
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.