RLG00000013224

Belongs to the ubiquitin-conjugating enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0005883
Physical Location & Seq
Forward (+)
27994685 .. 28001839
7155 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000013224

Sequence Viewer

Length: 3399 bp
ATGGGAAGCAAGCAGCATGACAGTGTTTTTAAAGACGATGAACCTACTACTGTAGACCATGATAATAGTTCTTTGATCCAGGTTGAGTTTTCAACTGATTCGAATGTGAATAATGAATTTAAAAAGTTGAGTGAGGTTGTCGGTGGCATGAATAATACTCCATACATATATAGACAAGATGTTGTGCGGAGCAAGAATGGTATGATTGGGGTTGTGACTGAAGTTGCTGGTGATTTGGATTCTGATAGTGGCATGTCTGATGATGAGGATGAGGATGATGATGAAGAAGAAGATGACAACGATGACGACGACCACAACGGTGAGAATCATGAAAATGACAAAGATGGTGGTGATGACAGTAATACTAATGAAAATGGTGACAAGAACCAGAATGGTGGTAATGATAAGACTGGTCCTCTTCCTGCAGACCAGGTTCGAGTGCTTTGGATAGATGAAACTGAGTCAACCCAGAATATCAATGATATAACAGTTGTTGATCGAGGATTCTTGCATGGAGATTTTGTTGCTGCTGCTTCTGAGCCAACCGGGCAAGTTGGTGTCGTGGTGGATGTCAATATCTCTGTTGATCTGAAAGCTCCTGATGGGCCCATCATAAAAGATGTGTCATCCAATGACTTAAAACGTGTGAGGGAGTTCACTGTCGGTGATTATGTGGTCCTTGGTACCTGGCTGGGTAGAATTGAGGATGTTTTTGATAATGTGACAGTGATGATTGATGATGGTTCCCTATGTAGAATTTTGAGAGCAGATCCGATGGATCTTAAACCACTTTCCAAAAATATGCTTGAAGATGTGCATTTCCCTTATTACCCAGGCCAGCGTGTGAAGGCAAGGTCATCATCGGTTTTCAAGAATTCTTTGTGGCTATCTGGCTCATGGAAGGCTAATCGTCTGGAAGGTACTGTTACTAAAGTTACAGTGGGATCAGTGTTTATTTATTGGATAGCTTCTGCTGGCTGTGGCCCAGATTCTTCTACTGCCCCTGCTAAAGAGCAGGTCCCCAGGAAGTTGAAACTATTGTCTTGTTTCACACATGCAAATTGGCAGTTGGGCGACTGGTGTCTTTTTCCATCATCAGGTTCGTCATCTTCCACTGCCGTAGATAAGGGTTCAAAATTAGAACTTCATCAATTAGATTCTACTCAAATGGGTAATGTGTGTGATTCGGAAGAGAGTTCACTAGAAGAATCAAATGGAAATACTGCAGCCATGGATACTGATCCAGTGTCTGTATTAGATGGAAACAATGGAAATGCTGGAAGTAATACTTCTATTGAATCTAGTTCCAGCGGTAGTTCATTGTCAGTCACAAAGGTGCCTGTTTCTCTTCATCGCAAAAAGCTGCGTAAACCTGTGGTTAAAAGAGACAAGAAGGCACGAAAGGAGGAAAATTTTGAAAGATCTTTTTTGATTGCAAATACCAGGACAACAGTTGATGTGGCATGGCAGGATGGTTCAACAGAACGGAAGGTGGCTTCAACCAATTTAATTCCTCTTGATAGTCCTGGTGATCATGAATTTGTTGCTGAACAATATGTGGTGGAGAAGGCATCTGATGATGGTGATGGTGCTTTTGATGCTAGGCGTGTTGGGCTCGTGAAAAGCGTCAATGCCAAGGAGCGAACAGCTTGTGTACAGTGGTTGAAGGCAGTTTCCAGAGCTGAAGATCCTAGAGAGTTTGACAAGGAGGAAGTTGTTAGTGTATATGAGCTCGAGGGTCACCCAGATTATGATTACTGTTATGGGGATGTTGTTGTCAGATTGTTGCCAGTTTCTGTTTCTGCACAAACTGCTTCGGTATCGGATTTAATTGAGGAGCCAAAGCAGCAAAATCAATCAAGTGAGCTTACATCAGAAGTTGTTGATGAAGCTTCTTCTGTTGATAAATCTTGTATGGACTTCTCAGATCTTTCTTGGGTTGGGAATATAACTGGTCTTAAGAATGGTGATATTGAAGTTACATGGGCTGACGGGATGGTTTCTACGGTTGGACCTCAAGCAATTTATGTTGTTGGTCGAGCTGATGATGATGACTCAATTGCTGGGAGTGAGGTTAGTGATGCTGCTAGTTGGGAAACAGTTGATGATGAGGAGGTGCATGCCCTATTCCCCCTCGAGAGTATAACAGAGGAAGTGGAGATACAGAGTGCCTTCAACATGAATTCTGAAGCAGAGGTGGGTGAAGAAGATAATTCTGGAAGGAACCCTGCATTGTCTGTTCCCTTGGCTGCACTTCGCTTTGTTACCAGACTGGCAAGTGGTATATTTGCTCGAGGACAAAAGAACTTGGATCCAATTTTAGATACTAAAGGTGAAGGTGATTTCGAGCCTCGGGAAGTTGAAATTTTTCAGGGAGATCATGGTGAAGATTCCAGCTCTCCAAAATCTAAAGTTGTTGATAGTAGTGTGGAGATCAATCATATAAATGAAGAGCATGTTTCCTCGGAGACTACAAAAATATTGGATGCAGCAGAAATCTTGTGCAACTTAAGGGCTGAAGGATCGGATGCTAAAGAAGTTAGCAAGGATGATGTTTGCAGTTTCAAACGCTTTGATATAGTCAAAGATCCTTTGGACCATCATTATCTTGGTGCAGCAGGGCAGAATGGCAGTAGTAAAAAGTGGTTCAAGAAGATTCAGCAAGACTGGGGCATCCTCCAAAATAACCTGCCTGATGGCATCTATGTACGTGTTTATGAAGATCGAATGGATCTCTTGAGGGCAGTTATTGTTGGGGCTTATGGGACACCTTACCAAGATGGCCTCTTCTTTTTCGATTTTCACCTTCCTCCAGAGTACCCTGATGTCCCTCCGTCAGCATATTATCATTCTGGTGGCTGGCGGATAAATCCAAATCTATACGAAGAAGGCAAAGTCTGTCTTAGTCTGTTAAATACTTGGACTGGCAGAGGAAATGAAGTATGGGATCCAAAGTCTTCTAGCATCCTTCAAGTTCTAGTTTCACTCCAGGGATTGGTGCTAAATTCTAAGCCCTATTTCAACGAAGCTGGCTATGATAAGCAAATTGGAACAGCTGAAGGAGAGAAAAATTCGTTGTCCTACAATGAGAATACTTTCTTGCTGAATTGCAAGACGATAATGTCTCTTATGCGGAGGCCTCCCAAGGACTTTGAAGAGCTTGTTCAAGACCATTTCAGGAGGCGCGGTTATTATATTCTCAAGGCTTGCGATGCGTATATGAAAGGCTACTTGATAGGTTCGCTCACTAAAGATGCCTCTGTTAATGATAGCAGTGATGCAAATTCGACATCTGTTGGTTTCAAGTTGATGTTAGCTAAGATTGTGCCAAAGCTGTTCTTGGCTTTGAGTGAGGTTGGAGCTGATTGTCATGAATTTAAGCATCTGCAACAGTCATAG

Protein Analysis

1133

Amino Acids

124.52

Weight (kDa)

4.54

Isoelectric Point (pI)

37.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SH3-A_UBE2O PF23048 56 - 165 1e-06 UBE2O, N-terminal SH3-A domain
tSH3-B_UBE2O PF23046 221 - 352 1.7e-42 UBE2O, tandem tSH3-B domain
SH3-B_UBE2O PF23043 511 - 583 4.1e-20 UBE2O, SH3-B domain
SH3-C_UBE2O PF23044 642 - 684 1.5e-06 UBE2O, ubiquitin binding SH3-C domain
UQ_con PF00179 886 - 1018 8.4e-20 Ubiquitin-conjugating enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 3121
Acc36I ACCTGC 2 cut(s) 1006, 2697
Acc65I GGTACC 1 cut(s) 683
AccB1I GGYRCC 2 cut(s) 683, 1336
AccB7I CCANNNNNTGG 1 cut(s) 2995
AccI GTMKAC 1 cut(s) 54
AccII CGCG 1 cut(s) 3186
AciI CCGC 5 cut(s) 187, 1311, 2863, 3133, 3186
AcuI CTGAAG 5 cut(s) 240, 1704, 2208, 2538, 3078
AfaI GTAC 5 cut(s) 685, 922, 1656, 2709, 2819
AfiI CCNNNNNNNGG 2 cut(s) 1097, 2995
AflII CTTAAG 2 cut(s) 1958, 2509
AflIII ACRYGT 2 cut(s) 643, 2710
AjnI CCWGG 8 cut(s) 78, 429, 686, 832, 1022, 1442, 1525, 2988
AleI CACNNNNGTG 2 cut(s) 318, 1334
Alw21I GWGCWC 1 cut(s) 1734
Alw26I GTCTC 3 cut(s) 1380, 2462, 3129
AlwNI CAGNNNCTG 2 cut(s) 1250, 1796
Ama87I CYCGRG 4 cut(s) 1733, 2135, 2292, 2352
AoxI GGCC 5 cut(s) 605, 835, 982, 2782, 3137
ApaI GGGCCC 1 cut(s) 609
Asp700I GAANNNNTTC 2 cut(s) 2367, 3095
Asp718I GGTACC 1 cut(s) 683
AspLEI GCGC 1 cut(s) 3186
AspS9I GGNCC 8 cut(s) 413, 605, 606, 676, 983, 1018, 2012, 2596
AsuC2I CCSGG 1 cut(s) 547
AsuII TTCGAA 1 cut(s) 101
AvaI CYCGRG 4 cut(s) 1733, 2135, 2292, 2352
AvaII GGWCC 5 cut(s) 413, 676, 1018, 2012, 2596
BaeGI GKGCMC 1 cut(s) 609
BamHI GGATCC 2 cut(s) 2311, 2947
BanI GGYRCC 2 cut(s) 683, 1336
BanII GRGCYC 3 cut(s) 609, 1617, 1734
BarI GAAGNNNNNNTAC 2 cut(s) 2145, 2177
BauI CACGAG 1 cut(s) 1616
BbsI GAAGAC 1 cut(s) 2949
Bbv12I GWGCWC 1 cut(s) 1734
BceAI ACGGC 1 cut(s) 1103
BciT130I CCWGG 8 cut(s) 80, 431, 688, 834, 1024, 1444, 1527, 2990
BciVI GTATCC 1 cut(s) 1228
BclI TGATCA 1 cut(s) 1531
BcnI CCSGG 1 cut(s) 547
BcoDI GTCTC 3 cut(s) 1380, 2462, 3129
BfaI CTAG 7 cut(s) 1202, 1302, 1602, 1692, 2088, 2961, 2978
BfmI CTRYAG 3 cut(s) 51, 423, 1224
BfrI CTTAAG 2 cut(s) 1958, 2509
BfuAI ACCTGC 2 cut(s) 1006, 2697
BfuI GTATCC 1 cut(s) 1228
BglI GCCNNNNNGGC 1 cut(s) 547
BglII AGATCT 2 cut(s) 1421, 1927
Bme1390I CCNGG 9 cut(s) 80, 431, 547, 688, 834, 1024, 1444, 1527, 2990
Bme18I GGWCC 5 cut(s) 413, 676, 1018, 2012, 2596
BmeT110I CYCGRG 4 cut(s) 1733, 2135, 2292, 2352
BmgT120I GGNCC 8 cut(s) 413, 605, 606, 676, 983, 1018, 2012, 2596
BmiI GGNNCC 9 cut(s) 607, 685, 745, 1020, 1338, 1839, 2225, 2313, 2949
BmrFI CCNGG 9 cut(s) 80, 431, 547, 688, 834, 1024, 1444, 1527, 2990
BmrI ACTGGG 1 cut(s) 2677
BmuI ACTGGG 1 cut(s) 2677
BoxI GACNNNNGTC 1 cut(s) 1080
BpiI GAAGAC 1 cut(s) 2949
BpmI CTGGAG 2 cut(s) 2796, 2972
Bpu14I TTCGAA 1 cut(s) 101
BpuEI CTTGAG 3 cut(s) 2001, 2758, 3185
BpuMI CCSGG 1 cut(s) 547
BsaAI YACGTR 1 cut(s) 2711
BsaBI GATNNNNATC 1 cut(s) 1239
BsaXI ACNNNNNCTCC 4 cut(s) 507, 537, 3172, 3202
Bsc4I CCNNNNNNNGG 2 cut(s) 1097, 2995
Bse1I ACTGG 8 cut(s) 415, 1082, 1244, 1790, 1957, 2277, 2672, 2929
Bse8I GATNNNNATC 1 cut(s) 1239
BseBI CCWGG 8 cut(s) 80, 431, 688, 834, 1024, 1444, 1527, 2990
BseJI GATNNNNATC 1 cut(s) 1239
BseLI CCNNNNNNNGG 2 cut(s) 1097, 2995
BseMII CTCAG 3 cut(s) 450, 528, 1938
BseNI ACTGG 8 cut(s) 415, 1082, 1244, 1790, 1957, 2277, 2672, 2929
BseRI GAGGAG 2 cut(s) 1850, 2126
BseSI GKGCMC 1 cut(s) 609
BseYI CCCAGC 2 cut(s) 691, 2063
BsgI GTGCAG 3 cut(s) 1788, 2235, 2634
Bsh1236I CGCG 1 cut(s) 3186
BshFI GGCC 5 cut(s) 607, 837, 984, 2784, 3139
BshNI GGYRCC 2 cut(s) 683, 1336
BsiHKAI GWGCWC 1 cut(s) 1734
BsiHKCI CYCGRG 4 cut(s) 1733, 2135, 2292, 2352
BsiSI CCGG 1 cut(s) 546
BslFI GGGAC 3 cut(s) 1004, 2779, 2813
BslI CCNNNNNNNGG 2 cut(s) 1097, 2995
BsmAI GTCTC 3 cut(s) 1380, 2462, 3129
BsmFI GGGAC 3 cut(s) 1004, 2779, 2813
BsnI GGCC 5 cut(s) 607, 837, 984, 2784, 3139
BsoBI CYCGRG 4 cut(s) 1733, 2135, 2292, 2352
Bsp119I TTCGAA 1 cut(s) 101
Bsp120I GGGCCC 1 cut(s) 605
Bsp1286I GDGCHC 3 cut(s) 609, 1617, 1734
Bsp1407I TGTACA 1 cut(s) 1654
Bsp19I CCATGG 1 cut(s) 1230
BspACI CCGC 5 cut(s) 187, 1311, 2863, 3133, 3186
BspANI GGCC 5 cut(s) 607, 837, 984, 2784, 3139
BspCNI CTCAG 3 cut(s) 451, 529, 1937
BspFNI CGCG 1 cut(s) 3186
BspHI TCATGA 3 cut(s) 328, 1534, 3370
BspLI GGNNCC 9 cut(s) 607, 685, 745, 1020, 1338, 1839, 2225, 2313, 2949
BspMAI CTGCAG 2 cut(s) 427, 1228
BspMI ACCTGC 2 cut(s) 1006, 2697
BspQI GCTCTTC 2 cut(s) 2445, 3150
BspT104I TTCGAA 1 cut(s) 101
BspT107I GGYRCC 2 cut(s) 683, 1336
BspTI CTTAAG 2 cut(s) 1958, 2509
BsrGI TGTACA 1 cut(s) 1654
BsrI ACTGG 8 cut(s) 415, 1082, 1244, 1790, 1957, 2277, 2672, 2929
BssSI CACGAG 1 cut(s) 1616
BssT1I CCWWGG 5 cut(s) 679, 1230, 1635, 2244, 3144
Bst2BI CACGAG 1 cut(s) 1616
Bst2UI CCWGG 8 cut(s) 80, 431, 688, 834, 1024, 1444, 1527, 2990
Bst6I CTCTTC 6 cut(s) 423, 1185, 1353, 2445, 2792, 3150
BstAFI CTTAAG 2 cut(s) 1958, 2509
BstAPI GCANNNNNTGC 1 cut(s) 1811
BstAUI TGTACA 1 cut(s) 1654
BstBAI YACGTR 1 cut(s) 2711
BstBI TTCGAA 1 cut(s) 101
BstC8I GCNNGC 7 cut(s) 11, 839, 976, 2121, 2861, 3031, 3208
BstDEI CTNAG 6 cut(s) 459, 537, 1924, 2903, 3009, 3318
BstDSI CCRYGG 1 cut(s) 1230
BstEII GGTNACC 1 cut(s) 1739
BstFNI CGCG 1 cut(s) 3186
BstHHI GCGC 1 cut(s) 3186
BstMAI GTCTC 3 cut(s) 1380, 2462, 3129
BstMWI GCNNNNNNNGC 6 cut(s) 547, 1598, 1612, 1811, 1846, 3212
BstNI CCWGG 8 cut(s) 80, 431, 688, 834, 1024, 1444, 1527, 2990
BstNSI RCATGY 4 cut(s) 256, 1058, 2123, 2459
BstPAI GACNNNNGTC 1 cut(s) 1080
BstPI GGTNACC 1 cut(s) 1739
BstSCI CCNGG 9 cut(s) 78, 429, 545, 686, 832, 1022, 1442, 1525, 2988
BstSFI CTRYAG 3 cut(s) 51, 423, 1224
BstSLI GKGCMC 1 cut(s) 609
BstUI CGCG 1 cut(s) 3186
BstV2I GAAGAC 1 cut(s) 2949
BstX2I RGATCY 9 cut(s) 769, 778, 1421, 1687, 1927, 2311, 2587, 2731, 2947
BstXI CCANNNNNNTGG 1 cut(s) 395
BstYI RGATCY 9 cut(s) 769, 778, 1421, 1687, 1927, 2311, 2587, 2731, 2947
BsuI GTATCC 1 cut(s) 1228
BsuRI GGCC 5 cut(s) 607, 837, 984, 2784, 3139
BtgI CCRYGG 1 cut(s) 1230
BtgZI GCGATG 2 cut(s) 1337, 3225
BtsI GCAGTG 2 cut(s) 1113, 3280
BtsIMutI CAGTG 9 cut(s) 28, 657, 732, 945, 954, 1113, 1251, 1664, 3280
BveI ACCTGC 2 cut(s) 1006, 2697
Cac8I GCNNGC 7 cut(s) 11, 839, 976, 2121, 2861, 3031, 3208
CaiI CAGNNNCTG 2 cut(s) 1250, 1796
CciI TCATGA 3 cut(s) 328, 1534, 3370
CfoI GCGC 1 cut(s) 3186
Cfr13I GGNCC 8 cut(s) 413, 605, 606, 676, 983, 1018, 2012, 2596
CseI GACGC 1 cut(s) 1615
CsiI ACCWGGT 1 cut(s) 429
Csp6I GTAC 5 cut(s) 684, 921, 1655, 2708, 2818
CspCI CAANNNNNGTGG 2 cut(s) 328, 363
CviQI GTAC 5 cut(s) 684, 921, 1655, 2708, 2818
DdeI CTNAG 6 cut(s) 459, 537, 1924, 2903, 3009, 3318
DraI TTTAAA 2 cut(s) 31, 121
DrdI GACNNNNNNGTC 1 cut(s) 3121
DseDI GACNNNNNNGTC 1 cut(s) 3121
Eam1104I CTCTTC 6 cut(s) 423, 1185, 1353, 2445, 2792, 3150
EarI CTCTTC 6 cut(s) 423, 1185, 1353, 2445, 2792, 3150
EciI GGCGGA 1 cut(s) 2878
Ecl136II GAGCTC 1 cut(s) 1732
Eco130I CCWWGG 5 cut(s) 679, 1230, 1635, 2244, 3144
Eco147I AGGCCT 1 cut(s) 3139
Eco24I GRGCYC 3 cut(s) 609, 1617, 1734
Eco47I GGWCC 5 cut(s) 413, 676, 1018, 2012, 2596
Eco53kI GAGCTC 1 cut(s) 1732
Eco57I CTGAAG 5 cut(s) 240, 1704, 2208, 2538, 3078
Eco88I CYCGRG 4 cut(s) 1733, 2135, 2292, 2352
Eco91I GGTNACC 1 cut(s) 1739
EcoICRI GAGCTC 1 cut(s) 1732
EcoO109I RGGNCCY 1 cut(s) 1018
EcoO65I GGTNACC 1 cut(s) 1739
EcoRI GAATTC 2 cut(s) 874, 2182
EcoRII CCWGG 8 cut(s) 78, 429, 686, 832, 1022, 1442, 1525, 2988
EcoT14I CCWWGG 5 cut(s) 679, 1230, 1635, 2244, 3144
EcoT38I GRGCYC 3 cut(s) 609, 1617, 1734
ErhI CCWWGG 5 cut(s) 679, 1230, 1635, 2244, 3144
FalI AAGNNNNNCTT 6 cut(s) 1273, 1305, 2886, 2918, 3323, 3355
FaqI GGGAC 3 cut(s) 1004, 2779, 2813
FbaI TGATCA 1 cut(s) 1531
FblI GTMKAC 1 cut(s) 54
FriOI GRGCYC 3 cut(s) 609, 1617, 1734
FspBI CTAG 7 cut(s) 1202, 1302, 1602, 1692, 2088, 2961, 2978
GlaI GCGC 1 cut(s) 3185
GsaI CCCAGC 2 cut(s) 695, 2067
GsuI CTGGAG 2 cut(s) 2796, 2972
HaeIII GGCC 5 cut(s) 607, 837, 984, 2784, 3139
HapII CCGG 1 cut(s) 546
HgaI GACGC 1 cut(s) 1615
HhaI GCGC 1 cut(s) 3186
Hin6I GCGC 1 cut(s) 3184
HinP1I GCGC 1 cut(s) 3184
HincII GTYRAC 1 cut(s) 465
HindII GTYRAC 1 cut(s) 465
HindIII AAGCTT 1 cut(s) 1890
HpaII CCGG 1 cut(s) 546
Hpy166II GTNNAC 6 cut(s) 55, 465, 657, 1199, 1370, 1655
Hpy8I GTNNAC 6 cut(s) 55, 465, 657, 1199, 1370, 1655
Hpy99I CGWCG 1 cut(s) 311
HpyCH4IV ACGT 2 cut(s) 643, 2710
HpyF10VI GCNNNNNNNGC 6 cut(s) 547, 1598, 1612, 1811, 1846, 3212
HpyF3I CTNAG 6 cut(s) 459, 537, 1924, 2903, 3009, 3318
HpySE526I ACGT 2 cut(s) 643, 2710
HspAI GCGC 1 cut(s) 3184
KpnI GGTACC 1 cut(s) 687
Ksp22I TGATCA 1 cut(s) 1531
LguI GCTCTTC 2 cut(s) 2445, 3150
LmnI GCTCC 5 cut(s) 189, 601, 1639, 1837, 3359
MabI ACCWGGT 1 cut(s) 429
MaeI CTAG 7 cut(s) 1202, 1302, 1602, 1692, 2088, 2961, 2978
MaeII ACGT 2 cut(s) 643, 2710
MaeIII GTNAC 9 cut(s) 214, 377, 721, 925, 934, 1327, 1739, 1978, 2263
MfeI CAATTG 1 cut(s) 2058
MflI RGATCY 9 cut(s) 769, 778, 1421, 1687, 1927, 2311, 2587, 2731, 2947
MhlI GDGCHC 3 cut(s) 609, 1617, 1734
MlyI GAGTC 2 cut(s) 470, 2048
MmeI TCCRAC 2 cut(s) 1990, 3337
MroXI GAANNNNTTC 2 cut(s) 2367, 3095
MslI CAYNNNNRTG 6 cut(s) 21, 318, 333, 1334, 2445, 2853
MspA1I CMGCKG 2 cut(s) 1311, 3056
MspCI CTTAAG 2 cut(s) 1958, 2509
MspI CCGG 1 cut(s) 546
MspR9I CCNGG 9 cut(s) 80, 431, 547, 688, 834, 1024, 1444, 1527, 2990
MunI CAATTG 1 cut(s) 2058
MvaI CCWGG 8 cut(s) 80, 431, 688, 834, 1024, 1444, 1527, 2990
MvnI CGCG 1 cut(s) 3186
MwoI GCNNNNNNNGC 6 cut(s) 547, 1598, 1612, 1811, 1846, 3212
NciI CCSGG 1 cut(s) 547
NcoI CCATGG 1 cut(s) 1230
NlaIV GGNNCC 9 cut(s) 607, 685, 745, 1020, 1338, 1839, 2225, 2313, 2949
NmuCI GTSAC 5 cut(s) 214, 377, 721, 1327, 1739
NspI RCATGY 4 cut(s) 256, 1058, 2123, 2459
NspV TTCGAA 1 cut(s) 101
OliI CACNNNNGTG 2 cut(s) 318, 1334
PaeI GCATGC 1 cut(s) 2123
PaeR7I CTCGAG 3 cut(s) 1733, 2135, 2292
PagI TCATGA 3 cut(s) 328, 1534, 3370
PceI AGGCCT 1 cut(s) 3139
PciSI GCTCTTC 2 cut(s) 2445, 3150
PcsI WCGNNNNNNNCGW 2 cut(s) 306, 1623
PdmI GAANNNNTTC 2 cut(s) 2367, 3095
PflMI CCANNNNNTGG 1 cut(s) 2995
PleI GAGTC 2 cut(s) 469, 2048
PpsI GAGTC 2 cut(s) 469, 2048
Ppu21I YACGTR 1 cut(s) 2711
PpuMI RGGWCCY 1 cut(s) 1018
PshAI GACNNNNGTC 1 cut(s) 1080
Psp124BI GAGCTC 1 cut(s) 1734
Psp5II RGGWCCY 1 cut(s) 1018
Psp6I CCWGG 8 cut(s) 78, 429, 686, 832, 1022, 1442, 1525, 2988
PspEI GGTNACC 1 cut(s) 1739
PspFI CCCAGC 2 cut(s) 691, 2063
PspGI CCWGG 8 cut(s) 78, 429, 686, 832, 1022, 1442, 1525, 2988
PspN4I GGNNCC 9 cut(s) 607, 685, 745, 1020, 1338, 1839, 2225, 2313, 2949
PspOMI GGGCCC 1 cut(s) 605
PspPI GGNCC 8 cut(s) 413, 605, 606, 676, 983, 1018, 2012, 2596
PspPPI RGGWCCY 1 cut(s) 1018
PspXI VCTCGAGB 2 cut(s) 1733, 2292
PstI CTGCAG 2 cut(s) 427, 1228
PstNI CAGNNNCTG 2 cut(s) 1250, 1796
PsuI RGATCY 9 cut(s) 769, 778, 1421, 1687, 1927, 2311, 2587, 2731, 2947
PvuII CAGCTG 1 cut(s) 3056
RsaI GTAC 5 cut(s) 685, 922, 1656, 2709, 2819
RsaNI GTAC 5 cut(s) 684, 921, 1655, 2708, 2818
RseI CAYNNNNRTG 6 cut(s) 21, 318, 333, 1334, 2445, 2853
SacI GAGCTC 1 cut(s) 1734
SapI GCTCTTC 2 cut(s) 2445, 3150
Sau96I GGNCC 8 cut(s) 413, 605, 606, 676, 983, 1018, 2012, 2596
SchI GAGTC 2 cut(s) 470, 2048
ScrFI CCNGG 9 cut(s) 80, 431, 547, 688, 834, 1024, 1444, 1527, 2990
SduI GDGCHC 3 cut(s) 609, 1617, 1734
SexAI ACCWGGT 1 cut(s) 429
SfcI CTRYAG 3 cut(s) 51, 423, 1224
Sfr274I CTCGAG 3 cut(s) 1733, 2135, 2292
SfuI TTCGAA 1 cut(s) 101
SinI GGWCC 5 cut(s) 413, 676, 1018, 2012, 2596
SlaI CTCGAG 3 cut(s) 1733, 2135, 2292
SmiMI CAYNNNNRTG 6 cut(s) 21, 318, 333, 1334, 2445, 2853
SmlI CTYRAG 8 cut(s) 1733, 1958, 2016, 2135, 2292, 2509, 2737, 3200
SmoI CTYRAG 8 cut(s) 1733, 1958, 2016, 2135, 2292, 2509, 2737, 3200
SphI GCATGC 1 cut(s) 2123
SseBI AGGCCT 1 cut(s) 3139
SsiI CCGC 5 cut(s) 187, 1311, 2863, 3133, 3186
SspI AATATT 1 cut(s) 2481
SspMI CTAG 7 cut(s) 1202, 1302, 1602, 1692, 2088, 2961, 2978
SstI GAGCTC 1 cut(s) 1734
StuI AGGCCT 1 cut(s) 3139
StyD4I CCNGG 9 cut(s) 78, 429, 545, 686, 832, 1022, 1442, 1525, 2988
StyI CCWWGG 5 cut(s) 679, 1230, 1635, 2244, 3144
TaiI ACGT 2 cut(s) 646, 2713
TatI WGTACW 1 cut(s) 1654
TscAI CASTG 9 cut(s) 28, 664, 732, 945, 954, 1120, 1251, 1664, 3280
TseFI GTSAC 5 cut(s) 214, 377, 721, 1327, 1739
Tsp45I GTSAC 5 cut(s) 214, 377, 721, 1327, 1739
TspGWI ACGGA 2 cut(s) 1501, 2823
TspRI CASTG 9 cut(s) 28, 664, 732, 945, 954, 1120, 1251, 1664, 3280
Van91I CCANNNNNTGG 1 cut(s) 2995
Vha464I CTTAAG 2 cut(s) 1958, 2509
VpaK11BI GGWCC 5 cut(s) 413, 676, 1018, 2012, 2596
XceI RCATGY 4 cut(s) 256, 1058, 2123, 2459
XcmI CCANNNNNNNNNTGG 1 cut(s) 2606
XhoI CTCGAG 3 cut(s) 1733, 2135, 2292
XmiI GTMKAC 1 cut(s) 54
XmnI GAANNNNTTC 2 cut(s) 2367, 3095
XspI CTAG 7 cut(s) 1202, 1302, 1602, 1692, 2088, 2961, 2978
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.