Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0005883

Physical Location & Seq

Genomic Coordinates Reverse (-)

20616557 .. 20623438

Gene Structure 6882 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1126200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3444 bp

ATGGGAACCAAGGAGCGTGACACTGGTTTTAAAGATGACGAGCCTACTACTATTGGCCACGATAATACTTCGTTGAGCCAGGTTGATTCTTCAACAAGCACACTTCTTTGCGATTCAAATGTAAATATTGAAAGTAATGAAGTTAAAAAGTTAAGTGAGGTTGTCAGCAACATTAACAATACCCCGTATATTTATAGACAAGACGTCGTGAGGAGCAAGAGTGGTATGACTGGGATTGTAACTGAGGTTGCTGGTGATTCAGATTCTGATAGTAGCATCATTGATGAGGAGGATGATGATGAAGATGATTACTACGATGATGATGAGAATGAGGAAGAAGGTGGTAATAACAATAATACTACTCATGGGAATAGTGACAAGAACAAAAATGGTAGTAATGATAAGACTGGTCCCCTTCCCACTGACCAAGTTCGAGTACTTTGGATGGATGAGACAGAGTCAACCCAAAATATTAATGATGTAACAGTTGTTGATCGAGGGTTCTTACATGGGGATTTTGTTGCTGCTGCTTCTGACCCAACTGGGCAAGTTGGTGTTGTGGTGGATGTCAATATCTCTGTTGATCTATTAGCTCCTGATGGGTCAGTCATAAAAGACCTTTCATCCAACGAATTAAAACGTGTGAGGGAGTTCACTGTGGGTGATTATGCGGTCCTTGGACCCTGGCTAGGTAGGATTGATGATGTTTATGATAATGTGACAGTTTTGTTTGATGATGGTTCTGTGTGTAAAGTTATGAAAGCACATCCGATGGACCTGAAACCAGTTTCCAAGAATATGCTTGAAGATGTGCATTTCCCTTACTACCCAGGGCAGCGTGTGAGGGCAAGGTCATCAGTTTTCAAGAATGCTAGGTGGCTATCTGGCTTGTGGAAACCTAATCGTGTGGAAGGTACTGTTACTAAGGTTACAGTTGCTTCAGTATTAATTTATTGGATAGCTTCTGCTGGCTGTGGGCCAGATTCTTCTACTGCACCTGCTAAAGAGCAGGTTCCCAAGAACTTGAAATTACTCTCTTGTTTTACACATGCAACTTGGCAATTGGGTGATTGGTGTCTTCTCCCCTCATCAGTGTCATCATCTTCCATTCCTTTAGACAAGGGATCATCAGAATTAGAACTTAATGATTCTGTAAGCAGTGAGTTAGAACCTAGTCAAATGGGTAGTAAGTGTGATTCAGAAGAGAGTGCGCTAGATGAATCAAATGGAAATCACGAATCCATGGAAATCGATCCAGTATCTGTATTAGATGGAAACAATGGAAATGCTGGAAGGAAGACATCTGTTGAATCTAGTTCCTGTAATAGTTCATTGTCAGTTTCAAAGGAGCCTGTTCATGAAACTTGGCCTCTTCATCGCAAAAAGATCCGCAAAGTTGTGGTTAGGAGAGACAAGAGGGCACGTAAGGAAGAAAATTTTGAAAGATCCTTTTTGATTGTCAATACCAAGACGACAGTTGACGTGGCATGGCAGGATGGCACAACAGAACTTAAACTGGACTCAAAAGATTTAATTCCGCTTGATAGTCCGGGTGATCACGAATTTGTTGCTGAACAGTATGTGGTGGAGAAGGCGACTGATGATGGTGATGATGCTTATGAAGCTAGGCATGTTGGGCTTGTGAAAAGTGTCAATGCCAAGGAGAGAACAGCTTGTGTAAGGTGGTTAAAGCCAGTTTCCAGACCGGAAGATCCTAGAGAGTTTGACAAGGAGGAAGTTGTGAGTGTCTATGAGCTTGAGGGGCATCCGGATTATGATTACTGTTATGGGGATGTTGTTGTCAGATTGTTGCCAGTTTTTGTCTCTGCCCAAACTGCTTCAGGTACAGAATTCGATGAGGAACCAAAGCAACAAAACGGACCAAGAGAGCTTAGTTCTGCTTCTACAAAAAAAGATCTATCTAGTGATGAAGCTTGTTTGGACTTCTCAGATCTTTCTTGGGTTGGAAATATAACTGGTCTTAAGAATGGGGATATTGAAGTTACATGGGCTGACGGGATGGTTTCTATGGTTGGACCTCAAGCAATATATGTTGTTGGCCGAGCTGATGATGATGACTCTATTGATGCTGGAAGTGATGTTAGTGATGCTGCTAGTTGGGAAACGGTTAATGATGATGAGACGCCTGCACTATCTGCCCCTGCGAGTACAGAAGAGGAAGTCCGGCTACAAAATTCCTTTGGTATGAATTTTGAAGCAGAAGAAAGTGGAGAGAACAATTCTGGAAGCAACCCGGCATTGTCTGTTCCCTTGGCTGCACTCAGGTTCGTTACCAGACTGGCGCATGGTATATTCTCAAGGGGACAAAGGAAATTGGATTCAAATTCTTCGGATAATGAAGGTGAGGGCGAAGGTGAAGTTGAGCCTCGGGAAGTGGAAATTTCTCAGGGAAGAGATCATGGTGAAGATTCCAGCTCTCAGAATTCTAATGTAGTGGATCCTTATGGTCTAGAAATAAATAAGGGAGAAGAAGAGAAAAATGTTACCCCACTGGCTGCAGAAATGTTGGATGAAGCAGAAGTTCTATGCAACTTAAAGACTGAAGAATCAAATTCTATAGAATGCACTAAGGATGATGCCTGCAGTTTCAAACGCTTTGATATTGCCAAAGATCCTTTGGATCATCATTTCCTTTGTGCAGCTGGGCAGAATACCAGTGGAAGAAAGTGGTTGAAGAAGGTTCAGCAAGATTGGAACATCCTTCAAAATAACCTTCCTGATGGGATTTGTGTACGAGTGTATGAAGACCGAATGGATCTCTTGAGGGCAGTTATAGTTGGGGCTTATGGGACACCTTACCAAGATGGCCTCTTCTTTTTTGATTTTCACCTCCCTCCGGAGTACCCTGATGTTCCTCCGACAGCATACTATCATTCTGGTGGCTGGCGGATAAATCCAAATCTATATGAAGAAGGCAAAGTGTGTCTTAGTCTTTTGAATACTTGGACTGGCAGAGGGAATGAAGTATGGGATCCAAAGTCTTCTAGCATCCTTCAAGTTCTTGTTTCACTCCAGGGGTTGGTGCTAAATTCTAAGCCATATTTTAATGAAGCTGGCTATGATAAGCAGGTCGGGACGGCTGAAGGAGAGAAAAATTCATTGTCCTACAATGAGAATACTTTCTTACTGAACTGCAAGACAATGATGTATCTTATGCGGAGGCCACCAAAGGACTTTGAAGAGCTAATCAATGACCATTTCCGGAGGCGTGGTTATTATATCTTGAAGGCCTGTGATGCATATATGAAAGGCTATTTGATTGGCTCTCTCACAAAAGATGCCTCTCTTAGTGATAAGAGCGATGTAAATTCAACTTCGGTTGGTTTCAAGTTGATGTTAGCTAAGATTGTGCCAAAGCTTTTCTCGGCGCTGAGTGCGGTTGGAGCTAATTGTCAAGAATTTATGCATCTGCAACAATCGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006464 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019899 GO:0031625 GO:0032446 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044389 GO:0044424 GO:0044444 GO:0044464 GO:0061630 GO:0061659 GO:0070647 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04120 map04120

Pfam Domains

Protein Families

UQ_con

Protein Analysis

1148

Amino Acids

126.66

Weight (kDa)

4.56

Isoelectric Point (pI)

39.92

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SH3-A_UBE2O	PF23048	64 - 164	5.3e-13	UBE2O, N-terminal SH3-A domain
tSH3-B_UBE2O	PF23046	220 - 350	4.1e-45	UBE2O, tandem tSH3-B domain
SH3-B_UBE2O	PF23043	519 - 591	4.9e-20	UBE2O, SH3-B domain
SH3-C_UBE2O	PF23044	652 - 692	5.7e-07	UBE2O, ubiquitin binding SH3-C domain
UQ_con	PF00179	901 - 1031	9.1e-20	Ubiquitin-conjugating enzyme

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0005883)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G16920 AT2G16920
fragaria_vesca	FvH4_2g15490 FvH4_2g15490 FvH4_2g15490 FvH4_2g15490 FvH4_2g15490 FvH4_2g15490 FvH4_2g15490
malus_domestica	MD05G1123200.v1.1 MD10G1126200.v1.1
prunus_persica	Prupe.8G167100_v2.0.a1 Prupe.8G167100_v2.0.a1 Prupe.8G167100_v2.0.a1
pyrus_communis	pycom05g11760 pycom10g10840
rosa_chinensis	RchiOBHm_Chr6g0278491
rosa_laevigata	RLG00000013224
rosa_multiflora	Rmu_sc0000258.1_g000006
rosa_roxburghii	Rroxscaffold_7G00189850 Rroxscaffold_7G00190340
rosa_rugosa	Rorug06G0117200 Rorug06G0117200
rosa_samantha	Rh6AG226500 Rh6BG230700 Rh6CG233800 Rh6DG225100
rosa_wichuraiana	Rw6G019770

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1006
AatII	GACGTC	1 cut(s)	207
Acc36I	ACCTGC	3 cut(s)	1000, 1006, 3079
AccB7I	CCANNNNNTGG	1 cut(s)	3040
AccIII	TCCGGA	3 cut(s)	1768, 2857, 3222
AciI	CCGC	6 cut(s)	671, 1390, 1538, 2908, 3178, 3398
AcoI	YGGCCR	2 cut(s)	55, 2059
AcuI	CTGAAG	4 cut(s)	924, 1824, 2583, 3123
AcyI	GRCGYC	2 cut(s)	204, 2144
AfaI	GTAC	6 cut(s)	438, 916, 1846, 2170, 2754, 2864
AfiI	CCNNNNNNNGG	3 cut(s)	689, 2857, 3040
AflII	CTTAAG	1 cut(s)	1982
AflIII	ACRYGT	1 cut(s)	640
AhdI	GACNNNNNGTC	1 cut(s)	203
AjiI	CACGTC	1 cut(s)	1483
AjnI	CCWGG	4 cut(s)	78, 683, 829, 3033
Alw26I	GTCTC	4 cut(s)	446, 1404, 1828, 2135
AlwNI	CAGNNNCTG	2 cut(s)	266, 1262
Ama87I	CYCGRG	1 cut(s)	2388
Aor13HI	TCCGGA	3 cut(s)	1768, 2857, 3222
AoxI	GGCC	7 cut(s)	55, 977, 1367, 2059, 2827, 3182, 3249
ApeKI	GCWGC	7 cut(s)	524, 527, 835, 2111, 2276, 2516, 2660
AseI	ATTAAT	2 cut(s)	474, 947
Asp700I	GAANNNNTTC	1 cut(s)	3140
AspLEI	GCGC	3 cut(s)	1213, 2305, 3391
AspS9I	GGNCC	7 cut(s)	410, 673, 680, 775, 977, 1880, 2036
AsuC2I	CCSGG	2 cut(s)	1551, 2255
AsuHPI	GGTGA	9 cut(s)	266, 674, 1079, 1565, 1619, 2375, 2387, 2435, 2840
AvaI	CYCGRG	1 cut(s)	2388
AvaII	GGWCC	6 cut(s)	410, 673, 680, 775, 1880, 2036
BaeGI	GKGCMC	1 cut(s)	1423
BalI	TGGCCA	1 cut(s)	57
BamHI	GGATCC	2 cut(s)	2458, 2992
BarI	GAAGNNNNNNTAC	2 cut(s)	2172, 2204
BbsI	GAAGAC	4 cut(s)	1070, 1304, 2772, 2994
BbvI	GCAGC	7 cut(s)	511, 514, 847, 2098, 2263, 2503, 2672
BceAI	ACGGC	1 cut(s)	3114
BcgI	CGANNNNNNTGC	2 cut(s)	1550, 1584
BciT130I	CCWGG	4 cut(s)	80, 685, 831, 3035
BclI	TGATCA	1 cut(s)	1555
BcnI	CCSGG	2 cut(s)	1551, 2255
BcoDI	GTCTC	4 cut(s)	446, 1404, 1828, 2135
BfmI	CTRYAG	3 cut(s)	2517, 2577, 2602
BfoI	RGCGCY	1 cut(s)	3392
BfrI	CTTAAG	1 cut(s)	1982
BfuAI	ACCTGC	3 cut(s)	1000, 1006, 3079
BglII	AGATCT	2 cut(s)	1915, 1951
BisI	GCNGC	7 cut(s)	525, 528, 836, 2112, 2277, 2517, 2661
BlsI	GCNGC	7 cut(s)	526, 529, 837, 2113, 2278, 2518, 2662
BmcAI	AGTACT	1 cut(s)	438
Bme1390I	CCNGG	6 cut(s)	80, 685, 831, 1551, 2255, 3035
Bme18I	GGWCC	6 cut(s)	410, 673, 680, 775, 1880, 2036
BmeRI	GACNNNNNGTC	1 cut(s)	203
BmeT110I	CYCGRG	1 cut(s)	2388
BmgBI	CACGTC	1 cut(s)	1483
BmgT120I	GGNCC	7 cut(s)	410, 673, 680, 775, 977, 1880, 2036
BmiI	GGNNCC	8 cut(s)	7, 412, 682, 1014, 1350, 1863, 2460, 2994
BmrFI	CCNGG	6 cut(s)	80, 685, 831, 1551, 2255, 3035
BmrI	ACTGGG	2 cut(s)	240, 552
BmuI	ACTGGG	2 cut(s)	240, 552
BpiI	GAAGAC	4 cut(s)	1070, 1304, 2772, 2994
BpmI	CTGGAG	1 cut(s)	3017
BpuEI	CTTGAG	4 cut(s)	1778, 2025, 2302, 2803
BpuMI	CCSGG	2 cut(s)	1551, 2255
Bsa29I	ATCGAT	1 cut(s)	1251
BsaAI	YACGTR	1 cut(s)	1424
BsaHI	GRCGYC	2 cut(s)	204, 2144
BsaWI	WCCGGW	4 cut(s)	1705, 1768, 2857, 3222
BsaXI	ACNNNNNCTCC	4 cut(s)	205, 235, 3217, 3247
Bsc4I	CCNNNNNNNGG	3 cut(s)	689, 2857, 3040
BseAI	TCCGGA	3 cut(s)	1768, 2857, 3222
BseBI	CCWGG	4 cut(s)	80, 685, 831, 3035
BseCI	ATCGAT	1 cut(s)	1251
BseLI	CCNNNNNNNGG	3 cut(s)	689, 2857, 3040
BseMII	CTCAG	6 cut(s)	234, 1962, 2296, 2420, 2453, 3383
BseRI	GAGGAG	2 cut(s)	226, 302
BseSI	GKGCMC	1 cut(s)	1423
BseXI	GCAGC	7 cut(s)	511, 514, 847, 2098, 2263, 2503, 2672
BseYI	CCCAGC	1 cut(s)	2663
BsgI	GTGCAG	4 cut(s)	978, 2133, 2262, 2679
BshFI	GGCC	7 cut(s)	57, 979, 1369, 2061, 2829, 3184, 3251
BshVI	ATCGAT	1 cut(s)	1251
BsiHKCI	CYCGRG	1 cut(s)	2388
BsiSI	CCGG	7 cut(s)	1550, 1706, 1769, 2185, 2255, 2858, 3223
BslFI	GGGAC	4 cut(s)	396, 2337, 2824, 3109
BslI	CCNNNNNNNGG	3 cut(s)	689, 2857, 3040
BsmAI	GTCTC	4 cut(s)	446, 1404, 1828, 2135
BsmBI	CGTCTC	1 cut(s)	2135
BsmFI	GGGAC	4 cut(s)	396, 2337, 2824, 3109
BsmI	GAATGC	2 cut(s)	874, 2588
BsnI	GGCC	7 cut(s)	57, 979, 1369, 2061, 2829, 3184, 3251
BsoBI	CYCGRG	1 cut(s)	2388
Bsp1286I	GDGCHC	1 cut(s)	1423
Bsp13I	TCCGGA	3 cut(s)	1768, 2857, 3222
Bsp19I	CCATGG	1 cut(s)	1242
BspACI	CCGC	6 cut(s)	671, 1390, 1538, 2908, 3178, 3398
BspANI	GGCC	7 cut(s)	57, 979, 1369, 2061, 2829, 3184, 3251
BspCNI	CTCAG	6 cut(s)	235, 1961, 2295, 2419, 2452, 3384
BspDI	ATCGAT	1 cut(s)	1251
BspEI	TCCGGA	3 cut(s)	1768, 2857, 3222
BspHI	TCATGA	1 cut(s)	1357
BspLI	GGNNCC	8 cut(s)	7, 412, 682, 1014, 1350, 1863, 2460, 2994
BspMAI	CTGCAG	2 cut(s)	2521, 2606
BspMI	ACCTGC	3 cut(s)	1000, 1006, 3079
BspQI	GCTCTTC	1 cut(s)	3195
BspTI	CTTAAG	1 cut(s)	1982
BssNI	GRCGYC	2 cut(s)	204, 2144
BssT1I	CCWWGG	5 cut(s)	9, 676, 1242, 1659, 2271
Bst2UI	CCWGG	4 cut(s)	80, 685, 831, 3035
Bst4CI	ACNGT	9 cut(s)	487, 658, 724, 919, 934, 1477, 1578, 1784, 2128
Bst6I	CTCTTC	7 cut(s)	1197, 1377, 2169, 2407, 2487, 2837, 3195
BstACI	GRCGYC	2 cut(s)	204, 2144
BstAFI	CTTAAG	1 cut(s)	1982
BstAPI	GCANNNNNTGC	1 cut(s)	2156
BstBAI	YACGTR	1 cut(s)	1424
BstC8I	GCNNGC	5 cut(s)	970, 2148, 2602, 2906, 3076
BstDSI	CCRYGG	1 cut(s)	1242
BstH2I	RGCGCY	1 cut(s)	3392
BstHHI	GCGC	3 cut(s)	1213, 2305, 3391
BstMAI	GTCTC	4 cut(s)	446, 1404, 1828, 2135
BstMWI	GCNNNNNNNGC	8 cut(s)	1622, 1636, 1762, 1835, 2156, 3257, 3395, 3404
BstNI	CCWGG	4 cut(s)	80, 685, 831, 3035
BstNSI	RCATGY	2 cut(s)	1052, 1634
BstSCI	CCNGG	6 cut(s)	78, 683, 829, 1549, 2253, 3033
BstSFI	CTRYAG	3 cut(s)	2517, 2577, 2602
BstSLI	GKGCMC	1 cut(s)	1423
BstV1I	GCAGC	7 cut(s)	511, 514, 847, 2098, 2263, 2503, 2672
BstV2I	GAAGAC	4 cut(s)	1070, 1304, 2772, 2994
BstX2I	RGATCY	9 cut(s)	1386, 1445, 1711, 1915, 1951, 2458, 2632, 2776, 2992
BstYI	RGATCY	9 cut(s)	1386, 1445, 1711, 1915, 1951, 2458, 2632, 2776, 2992
Bsu15I	ATCGAT	1 cut(s)	1251
BsuRI	GGCC	7 cut(s)	57, 979, 1369, 2061, 2829, 3184, 3251
BsuTUI	ATCGAT	1 cut(s)	1251
BtgI	CCRYGG	1 cut(s)	1242
BtgZI	GCGATG	2 cut(s)	1361, 3336
BtrI	CACGTC	1 cut(s)	1483
BtsI	GCAGTG	1 cut(s)	1165
BtsIMutI	CAGTG	7 cut(s)	21, 420, 654, 1098, 1165, 2510, 2683
BveI	ACCTGC	3 cut(s)	1000, 1006, 3079
Cac8I	GCNNGC	5 cut(s)	970, 2148, 2602, 2906, 3076
CaiI	CAGNNNCTG	2 cut(s)	266, 1262
CciI	TCATGA	1 cut(s)	1357
CfoI	GCGC	3 cut(s)	1213, 2305, 3391
Cfr13I	GGNCC	7 cut(s)	410, 673, 680, 775, 977, 1880, 2036
ClaI	ATCGAT	1 cut(s)	1251
CseI	GACGC	1 cut(s)	2152
Csp6I	GTAC	6 cut(s)	437, 915, 1845, 2169, 2753, 2863
CviQI	GTAC	6 cut(s)	437, 915, 1845, 2169, 2753, 2863
DraI	TTTAAA	1 cut(s)	31
DriI	GACNNNNNGTC	1 cut(s)	203
EaeI	YGGCCR	2 cut(s)	55, 2059
Eam1104I	CTCTTC	7 cut(s)	1197, 1377, 2169, 2407, 2487, 2837, 3195
Eam1105I	GACNNNNNGTC	1 cut(s)	203
EarI	CTCTTC	7 cut(s)	1197, 1377, 2169, 2407, 2487, 2837, 3195
EciI	GGCGGA	1 cut(s)	2923
Eco130I	CCWWGG	5 cut(s)	9, 676, 1242, 1659, 2271
Eco147I	AGGCCT	1 cut(s)	3251
Eco47I	GGWCC	6 cut(s)	410, 673, 680, 775, 1880, 2036
Eco57I	CTGAAG	4 cut(s)	924, 1824, 2583, 3123
Eco88I	CYCGRG	1 cut(s)	2388
EcoRI	GAATTC	2 cut(s)	1850, 2443
EcoRII	CCWGG	4 cut(s)	78, 683, 829, 3033
EcoT14I	CCWWGG	5 cut(s)	9, 676, 1242, 1659, 2271
EcoT22I	ATGCAT	2 cut(s)	3262, 3429
ErhI	CCWWGG	5 cut(s)	9, 676, 1242, 1659, 2271
Esp3I	CGTCTC	1 cut(s)	2135
FalI	AAGNNNNNCTT	2 cut(s)	2931, 2963
FaqI	GGGAC	4 cut(s)	396, 2337, 2824, 3109
FbaI	TGATCA	1 cut(s)	1555
Fnu4HI	GCNGC	7 cut(s)	525, 528, 836, 2112, 2277, 2517, 2661
Fsp4HI	GCNGC	7 cut(s)	525, 528, 836, 2112, 2277, 2517, 2661
GlaI	GCGC	3 cut(s)	1212, 2304, 3390
GluI	GCNGC	7 cut(s)	525, 528, 836, 2112, 2277, 2517, 2661
GsaI	CCCAGC	1 cut(s)	2667
GsuI	CTGGAG	1 cut(s)	3017
HaeII	RGCGCY	1 cut(s)	3392
HaeIII	GGCC	7 cut(s)	57, 979, 1369, 2061, 2829, 3184, 3251
HapII	CCGG	7 cut(s)	1550, 1706, 1769, 2185, 2255, 2858, 3223
HgaI	GACGC	1 cut(s)	2152
HhaI	GCGC	3 cut(s)	1213, 2305, 3391
Hin1I	GRCGYC	2 cut(s)	204, 2144
Hin6I	GCGC	3 cut(s)	1211, 2303, 3389
HinP1I	GCGC	3 cut(s)	1211, 2303, 3389
HincII	GTYRAC	2 cut(s)	462, 1480
HindII	GTYRAC	2 cut(s)	462, 1480
HindIII	AAGCTT	2 cut(s)	1932, 3377
HpaII	CCGG	7 cut(s)	1550, 1706, 1769, 2185, 2255, 2858, 3223
HphI	GGTGA	9 cut(s)	266, 674, 1079, 1565, 1619, 2375, 2387, 2435, 2840
Hpy166II	GTNNAC	4 cut(s)	462, 654, 1480, 2753
Hpy8I	GTNNAC	4 cut(s)	462, 654, 1480, 2753
Hpy99I	CGWCG	1 cut(s)	209
HpyCH4III	ACNGT	9 cut(s)	487, 658, 724, 919, 934, 1477, 1578, 1784, 2128
HpyCH4IV	ACGT	4 cut(s)	204, 640, 1423, 1482
HpyF10VI	GCNNNNNNNGC	8 cut(s)	1622, 1636, 1762, 1835, 2156, 3257, 3395, 3404
HpySE526I	ACGT	4 cut(s)	204, 640, 1423, 1482
Hsp92I	GRCGYC	2 cut(s)	204, 2144
HspAI	GCGC	3 cut(s)	1211, 2303, 3389
Kpn2I	TCCGGA	3 cut(s)	1768, 2857, 3222
Ksp22I	TGATCA	1 cut(s)	1555
LguI	GCTCTTC	1 cut(s)	3195
LmnI	GCTCC	5 cut(s)	13, 213, 598, 1348, 3404
Lsp1109I	GCAGC	7 cut(s)	511, 514, 847, 2098, 2263, 2503, 2672
MaeII	ACGT	4 cut(s)	204, 640, 1423, 1482
MfeI	CAATTG	1 cut(s)	1061
MflI	RGATCY	9 cut(s)	1386, 1445, 1711, 1915, 1951, 2458, 2632, 2776, 2992
MhlI	GDGCHC	1 cut(s)	1423
MlsI	TGGCCA	1 cut(s)	57
MluNI	TGGCCA	1 cut(s)	57
MlyI	GAGTC	3 cut(s)	467, 1514, 2072
MmeI	TCCRAC	6 cut(s)	651, 1945, 2014, 2508, 2903, 3382
Mox20I	TGGCCA	1 cut(s)	57
Mph1103I	ATGCAT	2 cut(s)	3262, 3429
MroI	TCCGGA	3 cut(s)	1768, 2857, 3222
MroXI	GAANNNNTTC	1 cut(s)	3140
MscI	TGGCCA	1 cut(s)	57
MslI	CAYNNNNRTG	1 cut(s)	2898
Msp20I	TGGCCA	1 cut(s)	57
MspA1I	CMGCKG	1 cut(s)	2663
MspCI	CTTAAG	1 cut(s)	1982
MspI	CCGG	7 cut(s)	1550, 1706, 1769, 2185, 2255, 2858, 3223
MspR9I	CCNGG	6 cut(s)	80, 685, 831, 1551, 2255, 3035
MunI	CAATTG	1 cut(s)	1061
Mva1269I	GAATGC	2 cut(s)	874, 2588
MvaI	CCWGG	4 cut(s)	80, 685, 831, 3035
MwoI	GCNNNNNNNGC	8 cut(s)	1622, 1636, 1762, 1835, 2156, 3257, 3395, 3404
NciI	CCSGG	2 cut(s)	1551, 2255
NcoI	CCATGG	1 cut(s)	1242
NlaIV	GGNNCC	8 cut(s)	7, 412, 682, 1014, 1350, 1863, 2460, 2994
NmeAIII	GCCGAG	2 cut(s)	2087, 3365
NmuCI	GTSAC	3 cut(s)	17, 374, 718
NsiI	ATGCAT	2 cut(s)	3262, 3429
NspI	RCATGY	2 cut(s)	1052, 1634
PagI	TCATGA	1 cut(s)	1357
PaqCI	CACCTGC	1 cut(s)	1006
PasI	CCCWGGG	1 cut(s)	830
PceI	AGGCCT	1 cut(s)	3251
PciSI	GCTCTTC	1 cut(s)	3195
PctI	GAATGC	2 cut(s)	874, 2588
PdmI	GAANNNNTTC	1 cut(s)	3140
PflFI	GACNNNGTC	1 cut(s)	457
PflMI	CCANNNNNTGG	1 cut(s)	3040
PkrI	GCNGC	7 cut(s)	526, 529, 837, 2113, 2278, 2518, 2662
PleI	GAGTC	3 cut(s)	466, 1514, 2072
PpsI	GAGTC	3 cut(s)	466, 1514, 2072
Ppu21I	YACGTR	1 cut(s)	1424
PshBI	ATTAAT	2 cut(s)	474, 947
Psp6I	CCWGG	4 cut(s)	78, 683, 829, 3033
PspFI	CCCAGC	1 cut(s)	2663
PspGI	CCWGG	4 cut(s)	78, 683, 829, 3033
PspN4I	GGNNCC	8 cut(s)	7, 412, 682, 1014, 1350, 1863, 2460, 2994
PspPI	GGNCC	7 cut(s)	410, 673, 680, 775, 977, 1880, 2036
PstI	CTGCAG	2 cut(s)	2521, 2606
PstNI	CAGNNNCTG	2 cut(s)	266, 1262
PsuI	RGATCY	9 cut(s)	1386, 1445, 1711, 1915, 1951, 2458, 2632, 2776, 2992
PsyI	GACNNNGTC	1 cut(s)	457
PvuII	CAGCTG	1 cut(s)	2663
RsaI	GTAC	6 cut(s)	438, 916, 1846, 2170, 2754, 2864
RsaNI	GTAC	6 cut(s)	437, 915, 1845, 2169, 2753, 2863
RseI	CAYNNNNRTG	1 cut(s)	2898
SapI	GCTCTTC	1 cut(s)	3195
SatI	GCNGC	7 cut(s)	525, 528, 836, 2112, 2277, 2517, 2661
Sau96I	GGNCC	7 cut(s)	410, 673, 680, 775, 977, 1880, 2036
ScaI	AGTACT	1 cut(s)	438
SchI	GAGTC	3 cut(s)	467, 1514, 2072
ScrFI	CCNGG	6 cut(s)	80, 685, 831, 1551, 2255, 3035
SduI	GDGCHC	1 cut(s)	1423
SfcI	CTRYAG	3 cut(s)	2517, 2577, 2602
SinI	GGWCC	6 cut(s)	410, 673, 680, 775, 1880, 2036
SmiMI	CAYNNNNRTG	1 cut(s)	2898
SmlI	CTYRAG	5 cut(s)	1757, 1982, 2040, 2317, 2782
SmoI	CTYRAG	5 cut(s)	1757, 1982, 2040, 2317, 2782
SseBI	AGGCCT	1 cut(s)	3251
SsiI	CCGC	6 cut(s)	671, 1390, 1538, 2908, 3178, 3398
SspI	AATATT	2 cut(s)	127, 472
StuI	AGGCCT	1 cut(s)	3251
StyD4I	CCNGG	6 cut(s)	78, 683, 829, 1549, 2253, 3033
StyI	CCWWGG	5 cut(s)	9, 676, 1242, 1659, 2271
TaaI	ACNGT	9 cut(s)	487, 658, 724, 919, 934, 1477, 1578, 1784, 2128
TaiI	ACGT	4 cut(s)	207, 643, 1426, 1485
TaqI	TCGA	4 cut(s)	433, 496, 1251, 1854
TaqII	GACCGA	1 cut(s)	2784
TatI	WGTACW	2 cut(s)	436, 2168
TscAI	CASTG	7 cut(s)	28, 427, 661, 1098, 1165, 2517, 2683
TseFI	GTSAC	3 cut(s)	17, 374, 718
TseI	GCWGC	7 cut(s)	524, 527, 835, 2111, 2276, 2516, 2660
Tsp45I	GTSAC	3 cut(s)	17, 374, 718
TspGWI	ACGGA	1 cut(s)	1893
TspRI	CASTG	7 cut(s)	28, 427, 661, 1098, 1165, 2517, 2683
Tth111I	GACNNNGTC	1 cut(s)	457
Van91I	CCANNNNNTGG	1 cut(s)	3040
Vha464I	CTTAAG	1 cut(s)	1982
VpaK11BI	GGWCC	6 cut(s)	410, 673, 680, 775, 1880, 2036
VspI	ATTAAT	2 cut(s)	474, 947
XbaI	TCTAGA	1 cut(s)	2470
XceI	RCATGY	2 cut(s)	1052, 1634
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2635
XmnI	GAANNNNTTC	1 cut(s)	3140
ZraI	GACGTC	1 cut(s)	205
ZrmI	AGTACT	1 cut(s)	438
Zsp2I	ATGCAT	2 cut(s)	3262, 3429

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.