Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr6B

Orthogroup ID

OG0005883

Physical Location & Seq

Genomic Coordinates Reverse (-)

43512205 .. 43520002

Gene Structure 7798 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh6BG230700.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3570 bp

ATGACTTCAACTCCTCCGCCCTCTCCTGCATTATATTACAAGACAAACCAAAACAGCTCTGCGACTTCCCTCCCTCTCAAAGAACCAAGCTTTCTTCTTCTTCTTCTTCTTCGTCTTCTTCCTTCACCTACCGCACCATCTCCATCTCCATCTCTTCCAACTCCTTCAGATTTTGGTATGGGAAGCAAGCAGCATGACAGTGTTTTTAAAGACGATGAACCTACTACTGTAGACCATGATAATAGTTCTTTGATCCGGGTTGAGTTTTCAACTGATTCAAATGTGAATAATGAATTTAAAAAGTTGAGTGAGGTTGTCGGTGGCATGAATAATACTCCATACATATATAGACAAGATGTTGTGAGGAGCAAGAATGGTATGATTGGGGTTGTGACTGAAGTTGCTGGTGATTTGGATTCTGATAGTGGCATGTCTGATGATGAGGATGAGGATGAGGATGAAATAGATGACGACGACGACGACCACAACAGTGAGAATCATGAAAATGACAAAGATGGTGGTGATGACAGTAATACTAATGAAAATGGTGACAAGAACCAGAATGGTGGTAATGATAAGACTGGTCCTCTTCCTGCGGACCAGGTTCAAGTGCTTTGGATAGATGAAACTGAGTCAACCCAGAATATCAATGATATAACAGTTGTTGATCGAGGATTCTTGCATGGAGATTTTGTTGCTGCTGCTTCTGAGCCAACCGGGCAAGTTGGTGTCGTGGTTGATGTCAATATCTCTGTTGATCTGAAAGCTCCTGACGGGTCCATCGTAAAAGATGTGTCATCCAATGACTTAAAACGTGTGAGGGAGTTCACTGTTGGTGATTATGTGGTCCTTGGTACCTGGCTGGGTAGAATTGAGGATGTTTTTGATAATGTGACGGTGATGATTGATGATGGTTCCCTATGTAGAATTTTGAGAGCAGATCCGATGGATCTTAAACCACTTTCCAAAAATATGCTTGAAGATGTGCATTTCCCTTATTACCCAGGCCAGCGTGTGAAGGCAAGGTCATCATCGGTTTTCAAGAATTCTTTGTGGCTATCCGGCTCATGGAAGGCTAATCGTCTGGAAGGTACTGTTACTAAAGTTACAGTGGGATCAGTGTTTATTTATTGGATAGCTTCTGCTGGCAGTGGCCCAGATTCTTCAACTGCCCCTGCTAAAGAGCAGGTCCCCAGGAAGTTGAAACTATTGTCTTGTTTCACACATGCAAATTGGCAGTTGGGCGACTGGTGTCTCTTTCCATCATCAGGGTCGTCATCTTCCACTGCCGTAGATAAGGGTTCAAAATTAGAACTTCATCAATTAGATTCTACTCAAATGGGTAATGTGTGTGATTCGGAAGAGAGTTCACTAGAAGAACCAAATGGAAATAGTGCATCCATGGATACTGACCCGGTGTCTGTATTAGATGGAAACAATGGAAATGCTGGAAGTAATACTTCCATTGAATCTAGTTCCAGCGGTAGTTCATTGTCAGTCACAAAGGTGCCTGTTTCTCTTCATCGCAAAAAGCTGCGTAAACCTGTGGTTAAAAGAGACAAGAAGGCACGAAAGGAGGAAAATTTTGAAAGATCTTTTTTGATTGCCAATTCCAGGACAACAGTTGATGTGGTATGGCAGGATGGTTCAACAGAACGGAAGCTGGCTTCAACGAATTTAATTCCTCTTGATAGTCCTGGTGATCATGAATTTGTTGCTGAACAATATGTGGTGGAGAAGGCATCTGATGATGGTGACAATGCTTTTGAAGCTAGGCGTGTTGGACTCGTGAAAAGCGTCAATGCCAAGGAGCGAACAGCTTGTGTACAGTGGTTGAAGGCAGTTTCCAGAGCTGAAGATCCTAGAGAGTTTGACAAGGAGGAAGTTGTTAGTGTATATGAGCTTGAGGGTCACCCAGATTATGATTACTGTTATGGGGATGTTGTTGTCAGATTGTTGCCAGTTTCTGTTTCTGCACAAACTGCTTCGGTATCGGACTTAATTGAGGAGCCAAAGCAGCAAAATCAACCAAGTGAGCTTACATCAGAAGTTGTTGATGAAGCTTCTTCTGTTGATAAATCTTGTATGGACTTCTCAGATCTTTCTTGGGTTGGGAATATAACTGGTCTTAAGAATGGTGATATTGAAGTTACATGGGCTGACGGGATGGTTTCTACGGTTGGACCTCAAGCAATTTATGTTGTTGGTCGAGCTGATGATGATGACTCAATTGCTGGGAGCGAGGTTAGTGATGCTGCTAGTTGGGAAACAGTTGATGATGAGGAGGTGCATGCCCTATTTCCCCTCGAGAGTATAACAGAGGAAGTGGAGATACAGAGTGCCTTTAACATGAATTCTGAAGCAGAGGTGGGTGGAGAAGATAATTCTGGAAGGAACCCTGCATTGTCTGTTCCCTTGGCTGCATTTCGCTTTGTTACCAGACTGGCAAGTGGAATATTTGCTCGGGGACAAAAGAACTTGGATCCAATTTTAGATACTAAAGGTGAAGGTGATTTTGAGCCTCGGGAAGTTGAAATCTTTCAGGGAGATCATTGTGAAGATTCCAGCTCTCAGAAATCTAAAGTTGTTGATATTAGTGTGGAGACCAATCATAAAAATGAAGAGCATGTTTCCTCGGAGACAACAAAAATATTGGATGCAGCAGAAATCTTGTGCAACTTAAGGACTGAAGGATTGGATGCTAAAGAATGTAGCAAGGGTGATGTTTGCAGTTTCAAACGCTTTGATATAGTCAAAGATCCTTTGGACCATCATTATCTTGGTGCAGCAGGGCAGGATAGCGGTGGTAAGAAGTGGTTCAAGAAGATTCAGCAAGATTGGGGCATCCTTCAAAATAACCTGCCTGATGGCATCTACGTACGCGTTTATGAAGATCGAATGGATCTCTTGAGGGCAGTTATAGTTGGGGCTTATGGGACACCTTACCAAGATGGCCTCTTCTTTTTCGATTTTCATCTCCCTCCGGAGTACCCTGATGTCCCTCCGTCAGCATATTATCATTCTGGTGGCTGGCGGATAAATCCAAATCTATACGAAGAAGGCAAAGTCTGTCTTAGTCTGTTAAATACTTGGACTGGTAGAGGAAATGAAGTTTGGGATCCAAAGTCTTCTAGCATCCTTCAAGTTCTAGTTTCACTCCAGGGATTGGTGCTAAATTCTAAGCCCTATTTCAACGAAGCTGGCTATGATAAGCAAATTGGAACAGCTGAAGGGGAGAAAAATTCGTTGTCCTACAATGAGAATACTTTCTTGCTGAATTGCAAGACGATAATGTCTCTTATGCGGAGGCCTCCCAAGGACTTTGAAGAGCTTGTTCAAGACCATTTCAGGAGGCGTGGTTATTATATCCTCAAGGCTTGCGATGCATATATGAAAGGCTACTTGATAGGTTCGCTCACTAAAGATGCCTCTGTTAGTGATAGCAGTGATGCAAATTCAACCTCTGTTGGTTTCAAGTTGATGTTAGCTAAGATTGTGCCAAAGCTGTTCTTGGCTTTGAGTGAGGTTGGAGCCGATTGTCATGAATTTAAGCATCTGCAACAGTCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006464 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019899 GO:0031625 GO:0032446 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044389 GO:0044424 GO:0044444 GO:0044464 GO:0061630 GO:0061659 GO:0070647 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04120 map04120

Pfam Domains

Protein Families

UQ_con

Protein Analysis

1189

Amino Acids

130.62

Weight (kDa)

4.59

Isoelectric Point (pI)

39.61

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
tSH3-B_UBE2O	PF23046	278 - 409	2.3e-42	UBE2O, tandem tSH3-B domain
SH3-B_UBE2O	PF23043	568 - 640	3.8e-20	UBE2O, SH3-B domain
SH3-C_UBE2O	PF23044	699 - 741	1.7e-06	UBE2O, ubiquitin binding SH3-C domain
UQ_con	PF00179	943 - 1075	9e-20	Ubiquitin-conjugating enzyme

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0005883)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G16920 AT2G16920
fragaria_vesca	FvH4_2g15490 FvH4_2g15490 FvH4_2g15490 FvH4_2g15490 FvH4_2g15490 FvH4_2g15490 FvH4_2g15490
malus_domestica	MD05G1123200.v1.1 MD10G1126200.v1.1
prunus_persica	Prupe.8G167100_v2.0.a1 Prupe.8G167100_v2.0.a1 Prupe.8G167100_v2.0.a1
pyrus_communis	pycom05g11760 pycom10g10840
rosa_chinensis	RchiOBHm_Chr6g0278491
rosa_laevigata	RLG00000013224
rosa_multiflora	Rmu_sc0000258.1_g000006
rosa_roxburghii	Rroxscaffold_7G00189850 Rroxscaffold_7G00190340
rosa_rugosa	Rorug06G0117200 Rorug06G0117200
rosa_samantha	Rh6AG226500 Rh6BG230700 Rh6CG233800 Rh6DG225100
rosa_wichuraiana	Rw6G019770

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	3292
Acc36I	ACCTGC	2 cut(s)	1177, 2868
Acc65I	GGTACC	1 cut(s)	854
AccB1I	GGYRCC	2 cut(s)	854, 1507
AccB7I	CCANNNNNTGG	1 cut(s)	3166
AccI	GTMKAC	1 cut(s)	231
AccII	CGCG	1 cut(s)	2883
AccIII	TCCGGA	1 cut(s)	2983
AciI	CCGC	7 cut(s)	17, 132, 596, 1482, 2802, 3034, 3304
AcuI	CTGAAG	6 cut(s)	150, 417, 1875, 2379, 2709, 3249
AfaI	GTAC	5 cut(s)	856, 1093, 1827, 2880, 2990
AfiI	CCNNNNNNNGG	4 cut(s)	1068, 1268, 1614, 3166
AflII	CTTAAG	2 cut(s)	2129, 2680
AflIII	ACRYGT	2 cut(s)	814, 2881
AhdI	GACNNNNNGTC	1 cut(s)	1417
AjnI	CCWGG	7 cut(s)	600, 857, 1003, 1193, 1613, 1696, 3159
AjuI	GAANNNNNNNTTGG	2 cut(s)	3097, 3129
AleI	CACNNNNGTG	2 cut(s)	489, 1505
Alw26I	GTCTC	5 cut(s)	1259, 1551, 2597, 2633, 3300
AlwNI	CAGNNNCTG	1 cut(s)	1967
Ama87I	CYCGRG	3 cut(s)	2306, 2463, 2523
Aor13HI	TCCGGA	1 cut(s)	2983
AoxI	GGCC	4 cut(s)	1006, 1153, 2953, 3308
ApeKI	GCWGC	9 cut(s)	190, 698, 701, 1534, 2017, 2255, 2420, 2660, 2786
ArsI	GACNNNNNNTTYG	2 cut(s)	1748, 1780
Asp700I	GAANNNNTTC	3 cut(s)	2538, 2816, 3266
Asp718I	GGTACC	1 cut(s)	854
AspS9I	GGNCC	8 cut(s)	584, 598, 777, 847, 1154, 1189, 2183, 2767
AsuC2I	CCSGG	3 cut(s)	257, 718, 1415
AvaI	CYCGRG	3 cut(s)	2306, 2463, 2523
AvaII	GGWCC	7 cut(s)	584, 598, 777, 847, 1189, 2183, 2767
BamHI	GGATCC	2 cut(s)	2482, 3118
BanI	GGYRCC	2 cut(s)	854, 1507
BarI	GAAGNNNNNNTAC	2 cut(s)	2316, 2348
BauI	CACGAG	1 cut(s)	1787
BbsI	GAAGAC	2 cut(s)	107, 3120
BbvI	GCAGC	9 cut(s)	202, 685, 688, 1521, 2029, 2242, 2407, 2672, 2798
BceAI	ACGGC	1 cut(s)	1274
BciT130I	CCWGG	7 cut(s)	602, 859, 1005, 1195, 1615, 1698, 3161
BciVI	GTATCC	1 cut(s)	1399
BclI	TGATCA	1 cut(s)	1702
BcnI	CCSGG	3 cut(s)	257, 718, 1415
BcoDI	GTCTC	5 cut(s)	1259, 1551, 2597, 2633, 3300
BfaI	CTAG	7 cut(s)	1373, 1473, 1773, 1863, 2259, 3132, 3149
BfmI	CTRYAG	1 cut(s)	228
BfrI	CTTAAG	2 cut(s)	2129, 2680
BfuAI	ACCTGC	2 cut(s)	1177, 2868
BfuI	GTATCC	1 cut(s)	1399
BglI	GCCNNNNNGGC	1 cut(s)	718
BglII	AGATCT	2 cut(s)	1592, 2098
BisI	GCNGC	9 cut(s)	191, 699, 702, 1535, 2018, 2256, 2421, 2661, 2787
BlsI	GCNGC	9 cut(s)	192, 700, 703, 1536, 2019, 2257, 2422, 2662, 2788
Bme18I	GGWCC	7 cut(s)	584, 598, 777, 847, 1189, 2183, 2767
BmeRI	GACNNNNNGTC	1 cut(s)	1417
BmeT110I	CYCGRG	3 cut(s)	2306, 2463, 2523
BmgT120I	GGNCC	8 cut(s)	584, 598, 777, 847, 1154, 1189, 2183, 2767
BoxI	GACNNNNGTC	1 cut(s)	1251
BpiI	GAAGAC	2 cut(s)	107, 3120
BpmI	CTGGAG	1 cut(s)	3143
BpuEI	CTTGAG	4 cut(s)	1925, 2172, 2929, 3356
BpuMI	CCSGG	3 cut(s)	257, 718, 1415
BsaAI	YACGTR	1 cut(s)	2878
BsaBI	GATNNNNATC	1 cut(s)	2973
BsaI	GGTCTC	1 cut(s)	2597
BsaWI	WCCGGW	1 cut(s)	2983
BsaXI	ACNNNNNCTCC	5 cut(s)	25, 678, 708, 3343, 3373
Bsc4I	CCNNNNNNNGG	4 cut(s)	1068, 1268, 1614, 3166
Bse1I	ACTGG	6 cut(s)	586, 1253, 1961, 2128, 2448, 3100
Bse8I	GATNNNNATC	1 cut(s)	2973
BseAI	TCCGGA	1 cut(s)	2983
BseBI	CCWGG	7 cut(s)	602, 859, 1005, 1195, 1615, 1698, 3161
BseJI	GATNNNNATC	1 cut(s)	2973
BseLI	CCNNNNNNNGG	4 cut(s)	1068, 1268, 1614, 3166
BseMII	CTCAG	4 cut(s)	621, 699, 2109, 2585
BseNI	ACTGG	6 cut(s)	586, 1253, 1961, 2128, 2448, 3100
BseRI	GAGGAG	3 cut(s)	379, 2021, 2297
BseXI	GCAGC	9 cut(s)	202, 685, 688, 1521, 2029, 2242, 2407, 2672, 2798
BseYI	CCCAGC	2 cut(s)	862, 2234
BsgI	GTGCAG	2 cut(s)	1959, 2805
Bsh1236I	CGCG	1 cut(s)	2883
BshFI	GGCC	4 cut(s)	1008, 1155, 2955, 3310
BshNI	GGYRCC	2 cut(s)	854, 1507
BsiHKCI	CYCGRG	3 cut(s)	2306, 2463, 2523
BsiSI	CCGG	5 cut(s)	256, 717, 1062, 1415, 2984
BsiWI	CGTACG	1 cut(s)	2878
BslFI	GGGAC	4 cut(s)	1175, 2481, 2950, 2984
BslI	CCNNNNNNNGG	4 cut(s)	1068, 1268, 1614, 3166
BsmAI	GTCTC	5 cut(s)	1259, 1551, 2597, 2633, 3300
BsmFI	GGGAC	4 cut(s)	1175, 2481, 2950, 2984
BsnI	GGCC	4 cut(s)	1008, 1155, 2955, 3310
Bso31I	GGTCTC	1 cut(s)	2597
BsoBI	CYCGRG	3 cut(s)	2306, 2463, 2523
Bsp13I	TCCGGA	1 cut(s)	2983
Bsp1407I	TGTACA	1 cut(s)	1825
Bsp19I	CCATGG	1 cut(s)	1401
BspACI	CCGC	7 cut(s)	17, 132, 596, 1482, 2802, 3034, 3304
BspANI	GGCC	4 cut(s)	1008, 1155, 2955, 3310
BspCNI	CTCAG	4 cut(s)	622, 700, 2108, 2584
BspEI	TCCGGA	1 cut(s)	2983
BspFNI	CGCG	1 cut(s)	2883
BspHI	TCATGA	3 cut(s)	499, 1705, 3541
BspMI	ACCTGC	2 cut(s)	1177, 2868
BspQI	GCTCTTC	2 cut(s)	2616, 3321
BspT107I	GGYRCC	2 cut(s)	854, 1507
BspTI	CTTAAG	2 cut(s)	2129, 2680
BspTNI	GGTCTC	1 cut(s)	2597
BsrGI	TGTACA	1 cut(s)	1825
BsrI	ACTGG	6 cut(s)	586, 1253, 1961, 2128, 2448, 3100
BssSI	CACGAG	1 cut(s)	1787
BssT1I	CCWWGG	5 cut(s)	850, 1401, 1806, 2415, 3315
Bst2BI	CACGAG	1 cut(s)	1787
Bst2UI	CCWGG	7 cut(s)	602, 859, 1005, 1195, 1615, 1698, 3161
Bst6I	CTCTTC	7 cut(s)	159, 594, 1356, 1524, 2616, 2963, 3321
BstAFI	CTTAAG	2 cut(s)	2129, 2680
BstAPI	GCANNNNNTGC	1 cut(s)	1982
BstAUI	TGTACA	1 cut(s)	1825
BstBAI	YACGTR	1 cut(s)	2878
BstC8I	GCNNGC	8 cut(s)	188, 1010, 1147, 1665, 2292, 3032, 3202, 3379
BstDEI	CTNAG	7 cut(s)	630, 708, 2095, 2571, 3074, 3180, 3489
BstDSI	CCRYGG	1 cut(s)	1401
BstEII	GGTNACC	1 cut(s)	1910
BstFNI	CGCG	1 cut(s)	2883
BstMAI	GTCTC	5 cut(s)	1259, 1551, 2597, 2633, 3300
BstMWI	GCNNNNNNNGC	5 cut(s)	718, 1769, 1982, 2017, 3383
BstNI	CCWGG	7 cut(s)	602, 859, 1005, 1195, 1615, 1698, 3161
BstNSI	RCATGY	4 cut(s)	433, 1229, 2294, 2630
BstPAI	GACNNNNGTC	1 cut(s)	1251
BstPI	GGTNACC	1 cut(s)	1910
BstSFI	CTRYAG	1 cut(s)	228
BstSNI	TACGTA	1 cut(s)	2878
BstUI	CGCG	1 cut(s)	2883
BstV1I	GCAGC	9 cut(s)	202, 685, 688, 1521, 2029, 2242, 2407, 2672, 2798
BstV2I	GAAGAC	2 cut(s)	107, 3120
BstX2I	RGATCY	9 cut(s)	940, 949, 1592, 1858, 2098, 2482, 2758, 2902, 3118
BstXI	CCANNNNNNTGG	1 cut(s)	566
BstYI	RGATCY	9 cut(s)	940, 949, 1592, 1858, 2098, 2482, 2758, 2902, 3118
BsuI	GTATCC	1 cut(s)	1399
BsuRI	GGCC	4 cut(s)	1008, 1155, 2955, 3310
BtgI	CCRYGG	1 cut(s)	1401
BtgZI	GCGATG	2 cut(s)	1508, 3396
BtsI	GCAGTG	3 cut(s)	1156, 1284, 3451
BtsIMutI	CAGTG	9 cut(s)	205, 496, 828, 1116, 1125, 1156, 1284, 1835, 3451
BveI	ACCTGC	2 cut(s)	1177, 2868
Cac8I	GCNNGC	8 cut(s)	188, 1010, 1147, 1665, 2292, 3032, 3202, 3379
CaiI	CAGNNNCTG	1 cut(s)	1967
CciI	TCATGA	3 cut(s)	499, 1705, 3541
Cfr13I	GGNCC	8 cut(s)	584, 598, 777, 847, 1154, 1189, 2183, 2767
CseI	GACGC	1 cut(s)	1786
CsiI	ACCWGGT	1 cut(s)	600
Csp6I	GTAC	5 cut(s)	855, 1092, 1826, 2879, 2989
CspCI	CAANNNNNGTGG	2 cut(s)	499, 534
CviQI	GTAC	5 cut(s)	855, 1092, 1826, 2879, 2989
DdeI	CTNAG	7 cut(s)	630, 708, 2095, 2571, 3074, 3180, 3489
DraI	TTTAAA	2 cut(s)	208, 298
DrdI	GACNNNNNNGTC	1 cut(s)	3292
DriI	GACNNNNNGTC	1 cut(s)	1417
DseDI	GACNNNNNNGTC	1 cut(s)	3292
Eam1104I	CTCTTC	7 cut(s)	159, 594, 1356, 1524, 2616, 2963, 3321
Eam1105I	GACNNNNNGTC	1 cut(s)	1417
EarI	CTCTTC	7 cut(s)	159, 594, 1356, 1524, 2616, 2963, 3321
EciI	GGCGGA	2 cut(s)	6, 3049
Eco105I	TACGTA	1 cut(s)	2878
Eco130I	CCWWGG	5 cut(s)	850, 1401, 1806, 2415, 3315
Eco147I	AGGCCT	1 cut(s)	3310
Eco31I	GGTCTC	1 cut(s)	2597
Eco47I	GGWCC	7 cut(s)	584, 598, 777, 847, 1189, 2183, 2767
Eco57I	CTGAAG	6 cut(s)	150, 417, 1875, 2379, 2709, 3249
Eco88I	CYCGRG	3 cut(s)	2306, 2463, 2523
Eco91I	GGTNACC	1 cut(s)	1910
EcoO109I	RGGNCCY	1 cut(s)	1189
EcoO65I	GGTNACC	1 cut(s)	1910
EcoRI	GAATTC	2 cut(s)	1045, 2353
EcoRII	CCWGG	7 cut(s)	600, 857, 1003, 1193, 1613, 1696, 3159
EcoT14I	CCWWGG	5 cut(s)	850, 1401, 1806, 2415, 3315
EcoT22I	ATGCAT	1 cut(s)	3388
ErhI	CCWWGG	5 cut(s)	850, 1401, 1806, 2415, 3315
FalI	AAGNNNNNCTT	6 cut(s)	1444, 1476, 3057, 3089, 3494, 3526
FaqI	GGGAC	4 cut(s)	1175, 2481, 2950, 2984
FbaI	TGATCA	1 cut(s)	1702
FblI	GTMKAC	1 cut(s)	231
Fnu4HI	GCNGC	9 cut(s)	191, 699, 702, 1535, 2018, 2256, 2421, 2661, 2787
Fsp4HI	GCNGC	9 cut(s)	191, 699, 702, 1535, 2018, 2256, 2421, 2661, 2787
FspBI	CTAG	7 cut(s)	1373, 1473, 1773, 1863, 2259, 3132, 3149
GluI	GCNGC	9 cut(s)	191, 699, 702, 1535, 2018, 2256, 2421, 2661, 2787
GsaI	CCCAGC	2 cut(s)	866, 2238
GsuI	CTGGAG	1 cut(s)	3143
HaeIII	GGCC	4 cut(s)	1008, 1155, 2955, 3310
HapII	CCGG	5 cut(s)	256, 717, 1062, 1415, 2984
HgaI	GACGC	1 cut(s)	1786
HincII	GTYRAC	1 cut(s)	636
HindII	GTYRAC	1 cut(s)	636
HindIII	AAGCTT	2 cut(s)	88, 2061
HpaII	CCGG	5 cut(s)	256, 717, 1062, 1415, 2984
Hpy166II	GTNNAC	6 cut(s)	232, 636, 828, 1370, 1541, 1826
Hpy8I	GTNNAC	6 cut(s)	232, 636, 828, 1370, 1541, 1826
Hpy99I	CGWCG	3 cut(s)	476, 479, 482
HpyCH4IV	ACGT	2 cut(s)	814, 2877
HpyF10VI	GCNNNNNNNGC	5 cut(s)	718, 1769, 1982, 2017, 3383
HpyF3I	CTNAG	7 cut(s)	630, 708, 2095, 2571, 3074, 3180, 3489
HpySE526I	ACGT	2 cut(s)	814, 2877
Kpn2I	TCCGGA	1 cut(s)	2983
KpnI	GGTACC	1 cut(s)	858
Ksp22I	TGATCA	1 cut(s)	1702
LguI	GCTCTTC	2 cut(s)	2616, 3321
LmnI	GCTCC	6 cut(s)	366, 772, 1810, 2008, 2238, 3530
Lsp1109I	GCAGC	9 cut(s)	202, 685, 688, 1521, 2029, 2242, 2407, 2672, 2798
MabI	ACCWGGT	1 cut(s)	600
MaeI	CTAG	7 cut(s)	1373, 1473, 1773, 1863, 2259, 3132, 3149
MaeII	ACGT	2 cut(s)	814, 2877
MfeI	CAATTG	1 cut(s)	2229
MflI	RGATCY	9 cut(s)	940, 949, 1592, 1858, 2098, 2482, 2758, 2902, 3118
MluI	ACGCGT	1 cut(s)	2881
MlyI	GAGTC	3 cut(s)	641, 1779, 2219
MmeI	TCCRAC	4 cut(s)	182, 1762, 2161, 3508
Mph1103I	ATGCAT	1 cut(s)	3388
MroI	TCCGGA	1 cut(s)	2983
MroXI	GAANNNNTTC	3 cut(s)	2538, 2816, 3266
MslI	CAYNNNNRTG	6 cut(s)	198, 489, 504, 1505, 2616, 3024
MspA1I	CMGCKG	2 cut(s)	1482, 3227
MspCI	CTTAAG	2 cut(s)	2129, 2680
MspI	CCGG	5 cut(s)	256, 717, 1062, 1415, 2984
MunI	CAATTG	1 cut(s)	2229
MvaI	CCWGG	7 cut(s)	602, 859, 1005, 1195, 1615, 1698, 3161
MvnI	CGCG	1 cut(s)	2883
MwoI	GCNNNNNNNGC	5 cut(s)	718, 1769, 1982, 2017, 3383
NciI	CCSGG	3 cut(s)	257, 718, 1415
NcoI	CCATGG	1 cut(s)	1401
NmuCI	GTSAC	6 cut(s)	391, 548, 892, 1498, 1754, 1910
NsiI	ATGCAT	1 cut(s)	3388
NspI	RCATGY	4 cut(s)	433, 1229, 2294, 2630
OliI	CACNNNNGTG	2 cut(s)	489, 1505
PaeI	GCATGC	1 cut(s)	2294
PaeR7I	CTCGAG	1 cut(s)	2306
PagI	TCATGA	3 cut(s)	499, 1705, 3541
PceI	AGGCCT	1 cut(s)	3310
PciSI	GCTCTTC	2 cut(s)	2616, 3321
PcsI	WCGNNNNNNNCGW	3 cut(s)	477, 780, 1794
PdmI	GAANNNNTTC	3 cut(s)	2538, 2816, 3266
Pfl23II	CGTACG	1 cut(s)	2878
PflMI	CCANNNNNTGG	1 cut(s)	3166
PfoI	TCCNGGA	1 cut(s)	1613
PkrI	GCNGC	9 cut(s)	192, 700, 703, 1536, 2019, 2257, 2422, 2662, 2788
PleI	GAGTC	3 cut(s)	640, 1779, 2219
PpsI	GAGTC	3 cut(s)	640, 1779, 2219
Ppu21I	YACGTR	1 cut(s)	2878
PpuMI	RGGWCCY	1 cut(s)	1189
PshAI	GACNNNNGTC	1 cut(s)	1251
Psp5II	RGGWCCY	1 cut(s)	1189
Psp6I	CCWGG	7 cut(s)	600, 857, 1003, 1193, 1613, 1696, 3159
PspEI	GGTNACC	1 cut(s)	1910
PspFI	CCCAGC	2 cut(s)	862, 2234
PspGI	CCWGG	7 cut(s)	600, 857, 1003, 1193, 1613, 1696, 3159
PspLI	CGTACG	1 cut(s)	2878
PspPI	GGNCC	8 cut(s)	584, 598, 777, 847, 1154, 1189, 2183, 2767
PspPPI	RGGWCCY	1 cut(s)	1189
PstNI	CAGNNNCTG	1 cut(s)	1967
PsuI	RGATCY	9 cut(s)	940, 949, 1592, 1858, 2098, 2482, 2758, 2902, 3118
PvuII	CAGCTG	1 cut(s)	3227
RsaI	GTAC	5 cut(s)	856, 1093, 1827, 2880, 2990
RsaNI	GTAC	5 cut(s)	855, 1092, 1826, 2879, 2989
RseI	CAYNNNNRTG	6 cut(s)	198, 489, 504, 1505, 2616, 3024
SapI	GCTCTTC	2 cut(s)	2616, 3321
SatI	GCNGC	9 cut(s)	191, 699, 702, 1535, 2018, 2256, 2421, 2661, 2787
Sau96I	GGNCC	8 cut(s)	584, 598, 777, 847, 1154, 1189, 2183, 2767
SchI	GAGTC	3 cut(s)	641, 1779, 2219
SexAI	ACCWGGT	1 cut(s)	600
SfcI	CTRYAG	1 cut(s)	228
Sfr274I	CTCGAG	1 cut(s)	2306
SinI	GGWCC	7 cut(s)	584, 598, 777, 847, 1189, 2183, 2767
SlaI	CTCGAG	1 cut(s)	2306
SmiMI	CAYNNNNRTG	6 cut(s)	198, 489, 504, 1505, 2616, 3024
SmlI	CTYRAG	7 cut(s)	1904, 2129, 2187, 2306, 2680, 2908, 3371
SmoI	CTYRAG	7 cut(s)	1904, 2129, 2187, 2306, 2680, 2908, 3371
SnaBI	TACGTA	1 cut(s)	2878
SphI	GCATGC	1 cut(s)	2294
SseBI	AGGCCT	1 cut(s)	3310
SsiI	CCGC	7 cut(s)	17, 132, 596, 1482, 2802, 3034, 3304
SspI	AATATT	2 cut(s)	2457, 2652
SspMI	CTAG	7 cut(s)	1373, 1473, 1773, 1863, 2259, 3132, 3149
StuI	AGGCCT	1 cut(s)	3310
StyI	CCWWGG	5 cut(s)	850, 1401, 1806, 2415, 3315
TaiI	ACGT	2 cut(s)	817, 2880
TaqI	TCGA	5 cut(s)	670, 2209, 2307, 2896, 2967
TatI	WGTACW	1 cut(s)	1825
TscAI	CASTG	9 cut(s)	205, 496, 835, 1116, 1125, 1156, 1291, 1835, 3451
TseFI	GTSAC	6 cut(s)	391, 548, 892, 1498, 1754, 1910
TseI	GCWGC	9 cut(s)	190, 698, 701, 1534, 2017, 2255, 2420, 2660, 2786
Tsp45I	GTSAC	6 cut(s)	391, 548, 892, 1498, 1754, 1910
TspGWI	ACGGA	2 cut(s)	1672, 2994
TspRI	CASTG	9 cut(s)	205, 496, 835, 1116, 1125, 1156, 1291, 1835, 3451
Van91I	CCANNNNNTGG	1 cut(s)	3166
Vha464I	CTTAAG	2 cut(s)	2129, 2680
VpaK11BI	GGWCC	7 cut(s)	584, 598, 777, 847, 1189, 2183, 2767
XceI	RCATGY	4 cut(s)	433, 1229, 2294, 2630
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2777
XhoI	CTCGAG	1 cut(s)	2306
XmiI	GTMKAC	1 cut(s)	231
XmnI	GAANNNNTTC	3 cut(s)	2538, 2816, 3266
XspI	CTAG	7 cut(s)	1373, 1473, 1773, 1863, 2259, 3132, 3149
Zsp2I	ATGCAT	1 cut(s)	3388

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.