AT2G20020

CRS2-associated factor 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0013996
Physical Location & Seq
Forward (+)
8644369 .. 8647291
2923 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G20020.1

Sequence Viewer

Length: 2106 bp
ATGTCGTTAAAACTCAACACTCCTTTTCCAATTTTCGCGCCATCTCTATTTCCTAATCATAACCCAAGAGCACCCAGCGAGATCCGATTCTCTAGATGGGGCAACGCTAATGCCGAACGGTTCGAGCAGCGTCGCCGGAGCCAAGAAGAACTCGAGGCTGAGATCCGTCGGGACCGCCGATTCGACGCCGCTACTAAAATCGTCCATACCCATGATTCCGAAGCAGCAGCTGCTGAGCCTAAAACGTCACCGTTTAGATCAAGAGGCACTCCTTCACTTCCCTCTGCTCGTTCGATTCCGGGTCGAAGATCCAAATACTCCAAACCCGATTCAGGACCCAATAGACCCAAGAACAAACCTAGAGTACCCGATTCGCCGCCGCAACTAGACGCTAAGCCTGAGGTTAAGCTAAGCGAAGATGGATTAACTTACGTCATCAATGGAGCTCCTTTCGAATTCAAGTACAGTTACACGGAGACGCCAAAGGTTAAGCCTTTGAAGCTTCGTGAGCCTGCTTACGCGCCTTTTGGACCTACGACTATGGGAAGGCCATGGACTGGTCGTGCTCCGCTTCCTCAGTCGCAGAAGACGCCGAGAGAATTCGATTCTTTTCGATTGCCTCCTGTGGGGAAGAAAGGGCTGAAGCCGGTGCAGAAACCGGGTCCTTTTCGACCCGGGGTAGGTCCAAGGTATGTTTACTCCAAGGAGGAGATTTTAGGAGAGCCATTGACAAAGGAAGAGGTCAGAGAGCTGGTTACTTCTTGCTTGAAGACAACAAGGCAATTGAATATGGGCAGAGATGGTTTGACGCATAACATGTTGAACAACATACATGATCTATGGAAGCGGCGAAGGGTTTGTAAGATTAAATGCAAAGGAGTTTGTACAGTCGATATGGATAATGTTTGCGAGCAGTTAGAGGAGAAAATTGGTGGGAAGGTGATATATAGAAGAGGAGGTGTGCTTTTCCTATTCCGTGGCAGAAACTATAACCACAGGACAAGACCGCGGTTCCCTCTTATGTTGTGGAAGCCTGTAGCACCTGTTTATCCAAGGCTAATTCAACAAGTGCCTGAGGGTTTAACTCGTCAGGAAGCTACCAATATGCGGAGGAAAGGACGAGAGCTCATGCCCATTTGCAAGCTAGGGAAGAATGGTGTGTATTGTGATCTTGTGAAAAATGTTAAAGAAGCATTTGAAGTTTGTGAATTGGTTCGGATCGATTGTCAAGGGATGAAAGGCAGTGATTTTAGGAAAATCGGTGCCAAACTCAAGGATCTTGTTCCATGTGTGCTCGTATCTTTTGAAAACGAGCAGATTCTTATCTGGAGAGGACGAGAATGGAAATCGTCTCTCACAACTCCAGATAAAAAGGGTGATATCCTTGAAGATATCGAAGTTGATACTGCCTTGCCAGAAGATGACGAACCATCGGTGTCACCAAATCAGAGTCAAACTATGACCCAGAACCCTCCTCTGGATTCTATGGAACTGCAAAATGATCCAGATGGTCACGATTTGAGCCCTTCAACTGTAGATTCCTCGGAAATGGAAGGCACAATCAATTCTTTACAGAGCTGGTCTACAAAAGATGTAACTGAGCCAACGGTAGATAGTTTTCTTCGAGACCTTGAAGAACCTGAAGACGAACCAGAAACATCGGAAGAGATCAGCAAACAAAGCATAGAGAGAGTTCTGATTTTGATGAAACAAGCTGTGGAGAGCGGGACTGCACTTGTGTTAGATGCTGCTGATCTGGACGCAGACACAGTCTTTTCAAAAGCTGTTGCCTTTTCGAGTGTAGCTTCACCAGGACCAGTTTTCCAGCATGGCTTGAGAAAACAACCAACGGTTAAGAAGCAGGAAAGCCAAGAATTCGGGTACGGAGACTTGGAGGCAAAATCAAGTAATGTAGTGGTTTCTAGGAATGCTTCCAAATCAAGTAATGTTGTGGTTTTTGGGAAAAGAGAAGTTGCAGAGAGGGGGGAAAGAGAGGAGAAGGAGGAGGGATCGAAGAAGAAAATGGACGAGTTTGCTGAAGATTACAGAGAAGTGATGCCGCATGGAACATTGAAGGTAGATGAACTAGCTAAACTACTTGCATAA

Protein Analysis

701

Amino Acids

79.05

Weight (kDa)

8.73

Isoelectric Point (pI)

57.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRS1_YhbY PF01985 243 - 326 1.3e-20 CRS1 / YhbY (CRM) domain
CRS1_YhbY PF01985 361 - 444 2.7e-17 CRS1 / YhbY (CRM) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013996)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G20020
fragaria_vesca FvH4_1g05780
malus_domestica MD02G1059500.v1.1 MD15G1193500.v1.1
prunus_persica Prupe.7G223600_v2.0.a1
pyrus_communis pycom02g04850 pycom15g17320
rosa_chinensis RchiOBHm_Chr2g0091431
rosa_laevigata RLG00000016223
rosa_multiflora Rmu_ssc0000333.1_g000008
rosa_roxburghii Rroxscaffold_2G00150090
rosa_rugosa Rorug02G0017800
rosa_samantha Rh2AG062700 Rh2BG062000 Rh2CG063900 Rh2DG062300
rosa_wichuraiana Rw2G005740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1262
AccB7I CCANNNNNTGG 1 cut(s) 557
AccBSI CCGCTC 1 cut(s) 1725
AccI GTMKAC 1 cut(s) 1583
AccII CGCG 3 cut(s) 38, 521, 1009
AclWI GGATC 7 cut(s) 76, 157, 303, 1226, 1284, 1496, 2017
AcsI RAATTY 3 cut(s) 455, 599, 1874
AcuI CTGAAG 3 cut(s) 662, 1662, 2058
AcyI GRCGYC 3 cut(s) 186, 479, 590
AfaI GTAC 4 cut(s) 366, 464, 886, 1883
AfiI CCNNNNNNNGG 6 cut(s) 332, 557, 626, 680, 1107, 1477
AflIII ACRYGT 1 cut(s) 816
AjnI CCWGG 1 cut(s) 1810
Alw21I GWGCWC 5 cut(s) 73, 448, 568, 1128, 1296
Alw26I GTCTC 4 cut(s) 470, 1356, 1620, 1881
AlwI GGATC 7 cut(s) 76, 157, 303, 1226, 1284, 1496, 2017
AlwNI CAGNNNCTG 2 cut(s) 230, 233
Ama87I CYCGRG 2 cut(s) 152, 674
AoxI GGCC 1 cut(s) 548
ApeKI GCWGC 5 cut(s) 127, 224, 227, 230, 1748
ApoI RAATTY 3 cut(s) 455, 599, 1874
ArsI GACNNNNNNTTYG 2 cut(s) 164, 196
Asp700I GAANNNNTTC 1 cut(s) 1212
AspLEI GCGC 2 cut(s) 40, 523
AspS9I GGNCC 6 cut(s) 172, 335, 530, 662, 683, 1814
AsuC2I CCSGG 4 cut(s) 300, 660, 675, 676
AsuHPI GGTGA 5 cut(s) 240, 952, 1388, 1431, 1800
AsuII TTCGAA 1 cut(s) 453
AvaI CYCGRG 2 cut(s) 152, 674
AvaII GGWCC 6 cut(s) 172, 335, 530, 662, 683, 1814
AxyI CCTNAGG 2 cut(s) 399, 1074
BanI GGYRCC 1 cut(s) 1262
BanII GRGCYC 3 cut(s) 448, 1128, 1526
BbsI GAAGAC 3 cut(s) 593, 776, 1650
Bbv12I GWGCWC 5 cut(s) 73, 448, 568, 1128, 1296
BbvI GCAGC 5 cut(s) 139, 217, 236, 239, 1735
BccI CCATC 6 cut(s) 49, 90, 413, 794, 1438, 1502
BciT130I CCWGG 1 cut(s) 1812
BcnI CCSGG 4 cut(s) 300, 660, 675, 676
BcoDI GTCTC 4 cut(s) 470, 1356, 1620, 1881
BfaI CTAG 6 cut(s) 93, 360, 386, 1145, 1923, 2087
BfmI CTRYAG 2 cut(s) 1035, 1533
BlpI GCTNAGC 3 cut(s) 234, 393, 410
Bme1390I CCNGG 5 cut(s) 300, 660, 675, 676, 1812
Bme18I GGWCC 6 cut(s) 172, 335, 530, 662, 683, 1814
BmeT110I CYCGRG 2 cut(s) 152, 674
BmgT120I GGNCC 6 cut(s) 172, 335, 530, 662, 683, 1814
BmiI GGNNCC 6 cut(s) 140, 173, 337, 663, 1013, 1264
BmrFI CCNGG 5 cut(s) 300, 660, 675, 676, 1812
BmsI GCATC 2 cut(s) 1735, 2046
BpiI GAAGAC 3 cut(s) 593, 776, 1650
BpmI CTGGAG 2 cut(s) 1347, 1348
Bpu1102I GCTNAGC 3 cut(s) 234, 393, 410
Bpu14I TTCGAA 1 cut(s) 453
BpuEI CTTGAG 2 cut(s) 1256, 1855
BpuMI CCSGG 4 cut(s) 300, 660, 675, 676
Bsa29I ATCGAT 1 cut(s) 1221
BsaBI GATNNNNATC 1 cut(s) 1322
BsaHI GRCGYC 3 cut(s) 186, 479, 590
BsaI GGTCTC 1 cut(s) 1620
BsaJI CCNNGG 9 cut(s) 551, 674, 675, 686, 702, 976, 1007, 1052, 1542
Bsc4I CCNNNNNNNGG 6 cut(s) 332, 557, 626, 680, 1107, 1477
Bse118I RCCGGY 1 cut(s) 646
Bse1I ACTGG 2 cut(s) 562, 1817
Bse21I CCTNAGG 2 cut(s) 399, 1074
Bse8I GATNNNNATC 1 cut(s) 1322
BseBI CCWGG 1 cut(s) 1812
BseCI ATCGAT 1 cut(s) 1221
BseDI CCNNGG 9 cut(s) 551, 674, 675, 686, 702, 976, 1007, 1052, 1542
BseGI GGATG 1 cut(s) 1239
BseJI GATNNNNATC 1 cut(s) 1322
BseLI CCNNNNNNNGG 6 cut(s) 332, 557, 626, 680, 1107, 1477
BseMII CTCAG 6 cut(s) 150, 225, 390, 590, 1065, 1590
BseNI ACTGG 2 cut(s) 562, 1817
BseRI GAGGAG 6 cut(s) 722, 935, 969, 1464, 2009, 2018
BseXI GCAGC 5 cut(s) 139, 217, 236, 239, 1735
BseYI CCCAGC 1 cut(s) 74
BsgI GTGCAG 2 cut(s) 671, 1716
Bsh1236I CGCG 3 cut(s) 38, 521, 1009
BshFI GGCC 1 cut(s) 550
BshNI GGYRCC 1 cut(s) 1262
BshVI ATCGAT 1 cut(s) 1221
BsiHKAI GWGCWC 5 cut(s) 73, 448, 568, 1128, 1296
BsiHKCI CYCGRG 2 cut(s) 152, 674
BsiSI CCGG 5 cut(s) 136, 299, 647, 659, 675
BslFI GGGAC 2 cut(s) 185, 1741
BslI CCNNNNNNNGG 6 cut(s) 332, 557, 626, 680, 1107, 1477
BsmAI GTCTC 4 cut(s) 470, 1356, 1620, 1881
BsmBI CGTCTC 2 cut(s) 470, 1356
BsmFI GGGAC 2 cut(s) 185, 1741
BsmI GAATGC 1 cut(s) 1933
BsnI GGCC 1 cut(s) 550
Bso31I GGTCTC 1 cut(s) 1620
BsoBI CYCGRG 2 cut(s) 152, 674
Bsp119I TTCGAA 1 cut(s) 453
Bsp1286I GDGCHC 6 cut(s) 73, 448, 568, 1128, 1296, 1526
Bsp1407I TGTACA 1 cut(s) 884
Bsp1720I GCTNAGC 3 cut(s) 234, 393, 410
Bsp19I CCATGG 1 cut(s) 551
BspANI GGCC 1 cut(s) 550
BspCNI CTCAG 6 cut(s) 151, 226, 391, 589, 1066, 1591
BspDI ATCGAT 1 cut(s) 1221
BspFNI CGCG 3 cut(s) 38, 521, 1009
BspLI GGNNCC 6 cut(s) 140, 173, 337, 663, 1013, 1264
BspPI GGATC 7 cut(s) 76, 157, 303, 1226, 1284, 1496, 2017
BspT104I TTCGAA 1 cut(s) 453
BspT107I GGYRCC 1 cut(s) 1262
BspTNI GGTCTC 1 cut(s) 1620
BsrBI CCGCTC 1 cut(s) 1725
BsrFI RCCGGY 1 cut(s) 646
BsrGI TGTACA 1 cut(s) 884
BsrI ACTGG 2 cut(s) 562, 1817
BssAI RCCGGY 1 cut(s) 646
BssECI CCNNGG 9 cut(s) 551, 674, 675, 686, 702, 976, 1007, 1052, 1542
BssNI GRCGYC 3 cut(s) 186, 479, 590
BssT1I CCWWGG 4 cut(s) 551, 686, 702, 1052
Bst2UI CCWGG 1 cut(s) 1812
Bst4CI ACNGT 8 cut(s) 120, 252, 467, 889, 1534, 1609, 1771, 1852
Bst6I CTCTTC 3 cut(s) 732, 946, 1659
BstACI GRCGYC 3 cut(s) 186, 479, 590
BstAPI GCANNNNNTGC 1 cut(s) 230
BstAUI TGTACA 1 cut(s) 884
BstBI TTCGAA 1 cut(s) 453
BstC8I GCNNGC 3 cut(s) 513, 911, 1142
BstDEI CTNAG 8 cut(s) 159, 234, 393, 399, 410, 576, 1074, 1599
BstDSI CCRYGG 3 cut(s) 551, 976, 1007
BstF5I GGATG 1 cut(s) 1239
BstFNI CGCG 3 cut(s) 38, 521, 1009
BstHHI GCGC 2 cut(s) 40, 523
BstMAI GTCTC 4 cut(s) 470, 1356, 1620, 1881
BstMWI GCNNNNNNNGC 5 cut(s) 230, 499, 508, 589, 1680
BstNI CCWGG 1 cut(s) 1812
BstNSI RCATGY 1 cut(s) 820
BstSCI CCNGG 5 cut(s) 298, 658, 673, 674, 1810
BstSFI CTRYAG 2 cut(s) 1035, 1533
BstUI CGCG 3 cut(s) 38, 521, 1009
BstV1I GCAGC 5 cut(s) 139, 217, 236, 239, 1735
BstV2I GAAGAC 3 cut(s) 593, 776, 1650
BstX2I RGATCY 4 cut(s) 81, 162, 308, 1276
BstYI RGATCY 4 cut(s) 81, 162, 308, 1276
Bsu15I ATCGAT 1 cut(s) 1221
Bsu36I CCTNAGG 2 cut(s) 399, 1074
BsuRI GGCC 1 cut(s) 550
BsuTUI ATCGAT 1 cut(s) 1221
BtgI CCRYGG 3 cut(s) 551, 976, 1007
BtsCI GGATG 1 cut(s) 1239
BtsI GCAGTG 1 cut(s) 1249
BtsIMutI CAGTG 1 cut(s) 1249
Cac8I GCNNGC 3 cut(s) 513, 911, 1142
CaiI CAGNNNCTG 2 cut(s) 230, 233
CfoI GCGC 2 cut(s) 40, 523
Cfr10I RCCGGY 1 cut(s) 646
Cfr13I GGNCC 6 cut(s) 172, 335, 530, 662, 683, 1814
Cfr42I CCGCGG 1 cut(s) 1010
Cfr9I CCCGGG 1 cut(s) 674
ClaI ATCGAT 1 cut(s) 1221
CseI GACGC 7 cut(s) 119, 194, 398, 487, 598, 817, 1769
Csp6I GTAC 4 cut(s) 365, 463, 885, 1882
CviAII CATG 8 cut(s) 212, 552, 817, 833, 1129, 1287, 1829, 2063
CviQI GTAC 4 cut(s) 365, 463, 885, 1882
DdeI CTNAG 8 cut(s) 159, 234, 393, 399, 410, 576, 1074, 1599
Eam1104I CTCTTC 3 cut(s) 732, 946, 1659
EarI CTCTTC 3 cut(s) 732, 946, 1659
Ecl136II GAGCTC 2 cut(s) 446, 1126
Eco130I CCWWGG 4 cut(s) 551, 686, 702, 1052
Eco24I GRGCYC 3 cut(s) 448, 1128, 1526
Eco31I GGTCTC 1 cut(s) 1620
Eco32I GATATC 2 cut(s) 1381, 1393
Eco47I GGWCC 6 cut(s) 172, 335, 530, 662, 683, 1814
Eco53kI GAGCTC 2 cut(s) 446, 1126
Eco57I CTGAAG 3 cut(s) 662, 1662, 2058
Eco81I CCTNAGG 2 cut(s) 399, 1074
Eco88I CYCGRG 2 cut(s) 152, 674
EcoICRI GAGCTC 2 cut(s) 446, 1126
EcoO109I RGGNCCY 2 cut(s) 335, 662
EcoRI GAATTC 3 cut(s) 455, 599, 1874
EcoRII CCWGG 1 cut(s) 1810
EcoRV GATATC 2 cut(s) 1381, 1393
EcoT14I CCWWGG 4 cut(s) 551, 686, 702, 1052
EcoT38I GRGCYC 3 cut(s) 448, 1128, 1526
ErhI CCWWGG 4 cut(s) 551, 686, 702, 1052
Esp3I CGTCTC 2 cut(s) 470, 1356
FaeI CATG 8 cut(s) 215, 555, 820, 836, 1132, 1290, 1832, 2066
FaqI GGGAC 2 cut(s) 185, 1741
FatI CATG 8 cut(s) 211, 551, 816, 832, 1128, 1286, 1828, 2062
FauI CCCGC 1 cut(s) 1718
FblI GTMKAC 1 cut(s) 1583
FokI GGATG 1 cut(s) 1246
FriOI GRGCYC 3 cut(s) 448, 1128, 1526
FspBI CTAG 6 cut(s) 93, 360, 386, 1145, 1923, 2087
GlaI GCGC 2 cut(s) 39, 522
GsaI CCCAGC 1 cut(s) 78
GsuI CTGGAG 2 cut(s) 1347, 1348
HaeIII GGCC 1 cut(s) 550
HapII CCGG 5 cut(s) 136, 299, 647, 659, 675
HgaI GACGC 7 cut(s) 119, 194, 398, 487, 598, 817, 1769
HhaI GCGC 2 cut(s) 40, 523
Hin1I GRCGYC 3 cut(s) 186, 479, 590
Hin1II CATG 8 cut(s) 215, 555, 820, 836, 1132, 1290, 1832, 2066
Hin6I GCGC 2 cut(s) 38, 521
HinP1I GCGC 2 cut(s) 38, 521
HindIII AAGCTT 1 cut(s) 500
HpaII CCGG 5 cut(s) 136, 299, 647, 659, 675
HphI GGTGA 5 cut(s) 240, 952, 1388, 1431, 1800
Hpy166II GTNNAC 2 cut(s) 697, 1584
Hpy188I TCNGA 8 cut(s) 86, 220, 746, 1218, 1449, 1546, 1663, 1698
Hpy8I GTNNAC 2 cut(s) 697, 1584
Hpy99I CGWCG 3 cut(s) 135, 171, 188
HpyAV CCTTC 8 cut(s) 282, 540, 846, 931, 1536, 1547, 1993, 2068
HpyCH4III ACNGT 8 cut(s) 120, 252, 467, 889, 1534, 1609, 1771, 1852
HpyCH4IV ACGT 2 cut(s) 245, 432
HpyCH4V TGCA 7 cut(s) 652, 873, 1140, 1495, 1733, 1976, 2102
HpyF10VI GCNNNNNNNGC 5 cut(s) 230, 499, 508, 589, 1680
HpyF3I CTNAG 8 cut(s) 159, 234, 393, 399, 410, 576, 1074, 1599
HpySE526I ACGT 2 cut(s) 245, 432
Hsp92I GRCGYC 3 cut(s) 186, 479, 590
Hsp92II CATG 8 cut(s) 215, 555, 820, 836, 1132, 1290, 1832, 2066
HspAI GCGC 2 cut(s) 38, 521
KspI CCGCGG 1 cut(s) 1010
LmnI GCTCC 4 cut(s) 138, 443, 451, 571
Lsp1109I GCAGC 5 cut(s) 139, 217, 236, 239, 1735
LweI GCATC 2 cut(s) 1735, 2046
MaeI CTAG 6 cut(s) 93, 360, 386, 1145, 1923, 2087
MaeII ACGT 2 cut(s) 245, 432
MaeIII GTNAC 6 cut(s) 246, 467, 754, 1437, 1511, 1594
MbiI CCGCTC 1 cut(s) 1725
MfeI CAATTG 1 cut(s) 782
MflI RGATCY 4 cut(s) 81, 162, 308, 1276
MhlI GDGCHC 6 cut(s) 73, 448, 568, 1128, 1296, 1526
MluCI AATT 9 cut(s) 30, 455, 599, 782, 927, 1059, 1208, 1564, 1874
MlyI GAGTC 1 cut(s) 1459
MroXI GAANNNNTTC 1 cut(s) 1212
MseI TTAA 8 cut(s) 8, 405, 425, 489, 867, 1082, 1185, 1854
MslI CAYNNNNRTG 1 cut(s) 210
MspA1I CMGCKG 2 cut(s) 230, 1009
MspI CCGG 5 cut(s) 136, 299, 647, 659, 675
MspR9I CCNGG 5 cut(s) 300, 660, 675, 676, 1812
MunI CAATTG 1 cut(s) 782
Mva1269I GAATGC 1 cut(s) 1933
MvaI CCWGG 1 cut(s) 1812
MvnI CGCG 3 cut(s) 38, 521, 1009
MwoI GCNNNNNNNGC 5 cut(s) 230, 499, 508, 589, 1680
NciI CCSGG 4 cut(s) 300, 660, 675, 676
NcoI CCATGG 1 cut(s) 551
NlaIII CATG 8 cut(s) 215, 555, 820, 836, 1132, 1290, 1832, 2066
NlaIV GGNNCC 6 cut(s) 140, 173, 337, 663, 1013, 1264
NmeAIII GCCGAG 1 cut(s) 618
NmuCI GTSAC 3 cut(s) 246, 1437, 1511
NspI RCATGY 1 cut(s) 820
NspV TTCGAA 1 cut(s) 453
PaeR7I CTCGAG 1 cut(s) 152
PciI ACATGT 1 cut(s) 816
PcsI WCGNNNNNNNCGW 2 cut(s) 111, 175
PctI GAATGC 1 cut(s) 1933
PdmI GAANNNNTTC 1 cut(s) 1212
PflFI GACNNNGTC 1 cut(s) 1769
PflMI CCANNNNNTGG 1 cut(s) 557
PleI GAGTC 1 cut(s) 1458
PpsI GAGTC 1 cut(s) 1458
PpuMI RGGWCCY 2 cut(s) 335, 662
PscI ACATGT 1 cut(s) 816
Psp124BI GAGCTC 2 cut(s) 448, 1128
Psp5II RGGWCCY 2 cut(s) 335, 662
Psp6I CCWGG 1 cut(s) 1810
PspFI CCCAGC 1 cut(s) 74
PspGI CCWGG 1 cut(s) 1810
PspN4I GGNNCC 6 cut(s) 140, 173, 337, 663, 1013, 1264
PspPI GGNCC 6 cut(s) 172, 335, 530, 662, 683, 1814
PspPPI RGGWCCY 2 cut(s) 335, 662
PspXI VCTCGAGB 1 cut(s) 152
PstNI CAGNNNCTG 2 cut(s) 230, 233
PsuI RGATCY 4 cut(s) 81, 162, 308, 1276
PsyI GACNNNGTC 1 cut(s) 1769
PvuII CAGCTG 1 cut(s) 230
RsaI GTAC 4 cut(s) 366, 464, 886, 1883
RsaNI GTAC 4 cut(s) 365, 463, 885, 1882
RseI CAYNNNNRTG 1 cut(s) 210
SacI GAGCTC 2 cut(s) 448, 1128
SacII CCGCGG 1 cut(s) 1010
SaqAI TTAA 8 cut(s) 8, 405, 425, 489, 867, 1082, 1185, 1854
Sau96I GGNCC 6 cut(s) 172, 335, 530, 662, 683, 1814
SchI GAGTC 1 cut(s) 1459
ScrFI CCNGG 5 cut(s) 300, 660, 675, 676, 1812
SduI GDGCHC 6 cut(s) 73, 448, 568, 1128, 1296, 1526
SfaNI GCATC 2 cut(s) 1735, 2046
SfcI CTRYAG 2 cut(s) 1035, 1533
Sfr274I CTCGAG 1 cut(s) 152
Sfr303I CCGCGG 1 cut(s) 1010
SfuI TTCGAA 1 cut(s) 453
SgrBI CCGCGG 1 cut(s) 1010
SinI GGWCC 6 cut(s) 172, 335, 530, 662, 683, 1814
SlaI CTCGAG 1 cut(s) 152
SmaI CCCGGG 1 cut(s) 676
SmiMI CAYNNNNRTG 1 cut(s) 210
SmlI CTYRAG 3 cut(s) 152, 1271, 1834
SmoI CTYRAG 3 cut(s) 152, 1271, 1834
Sse9I AATT 9 cut(s) 30, 455, 599, 782, 927, 1059, 1208, 1564, 1874
SspMI CTAG 6 cut(s) 93, 360, 386, 1145, 1923, 2087
SstI GAGCTC 2 cut(s) 448, 1128
StyD4I CCNGG 5 cut(s) 298, 658, 673, 674, 1810
StyI CCWWGG 4 cut(s) 551, 686, 702, 1052
TaaI ACNGT 8 cut(s) 120, 252, 467, 889, 1534, 1609, 1771, 1852
TaiI ACGT 2 cut(s) 248, 435
TasI AATT 9 cut(s) 30, 455, 599, 782, 927, 1059, 1208, 1564, 1874
TatI WGTACW 2 cut(s) 462, 884
TauI GCSGC 5 cut(s) 191, 379, 382, 850, 2062
Tru1I TTAA 8 cut(s) 8, 405, 425, 489, 867, 1082, 1185, 1854
Tru9I TTAA 8 cut(s) 8, 405, 425, 489, 867, 1082, 1185, 1854
TscAI CASTG 1 cut(s) 1249
TseFI GTSAC 3 cut(s) 246, 1437, 1511
TseI GCWGC 5 cut(s) 127, 224, 227, 230, 1748
Tsp45I GTSAC 3 cut(s) 246, 1437, 1511
TspDTI ATGAA 3 cut(s) 1250, 1721, 2097
TspGWI ACGGA 4 cut(s) 155, 488, 965, 1899
TspMI CCCGGG 1 cut(s) 674
TspRI CASTG 1 cut(s) 1249
Tth111I GACNNNGTC 1 cut(s) 1769
Van91I CCANNNNNTGG 1 cut(s) 557
VpaK11BI GGWCC 6 cut(s) 172, 335, 530, 662, 683, 1814
XapI RAATTY 3 cut(s) 455, 599, 1874
XbaI TCTAGA 1 cut(s) 92
XceI RCATGY 1 cut(s) 820
XhoI CTCGAG 1 cut(s) 152
XmaI CCCGGG 1 cut(s) 674
XmiI GTMKAC 1 cut(s) 1583
XmnI GAANNNNTTC 1 cut(s) 1212
XspI CTAG 6 cut(s) 93, 360, 386, 1145, 1923, 2087
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.