Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0013996

Physical Location & Seq

Genomic Coordinates Reverse (-)

15247180 .. 15250363

Gene Structure 3184 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1193500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2325 bp

ATGGCGCTAAATTTGCCCACTTCCTGTCCCATTTTCGCGCCACTATTGAACCCGAGCCCGACCCACAACAACCCGAATCAGCACCGCCCTCCAACGGAGGTCCGGTTCGCTCGGTGGAACAACGCCAACGCCGAGAAATTCAACCAGCGCCGGAGGGCCCAGCAAGAAATCGAGGACGACATTCGCCGGGAGCGCAGATTCGAGTCCGCCACCAGAATTGCCACTCTTTCCGACTCTGCCACTGACACCACCGCCGCCGCGGCCAACGCCACCACAAGTAGTGAAACATATAAATCCATCGGCACGCCGTCGTATCCCTCGAGCCCTTCAATCCCAGGCAAGAAATCCAAGTACTCTAAAAACCCTAACCCTACCGATTCGCACCCCGCATTCCGCCGAGTCATACGACCCACGAAGCTCTCGAAAATCCCGAGGGAGAAAAAACCGGCAGTCGACAGAAAAGCCGACATTTCCATCGGCGACGATGGGCTTTCTTACGTCATCGACGGAGCCCCGTTTGAATTCAAGTACAGCTACACCGAGACCCCTAAAATCAAGCCCATTAAGCTCCGGGAACCGCCGTTCACGCCATTCGGGCCCACCACGATGGACCGGCCATGGACGGGTCGGGCCCCATTGCCGGCGAGCAAGAAGAAATTGAAGGAATTCGACTCGTTCCAGCTGCCTCCGCCGCACAAGAAGGGGGTTAAGCCGGTGCAATCTCCGGGTCCGTATTTGCCCGGATCGGGTCCTAAGTATGTGAAGTCCAGGGAGGAGATTTTGGGAGATCCCCTGACGGTTGAGGAAGTTAAGGAGTTAGTTAAAGGGTGCATCAAAACCAAGAGGCAGTTGAATATGGGTAGAGATGGTTTGACTCACAACATGTTGGATAACATACATGCTCACTGGAAGCGCCGGAGAGTGTGCAAGATTAAATGCAAAGGAGTTTGTACAGTCGACATGGAGAATGTGTGCGAGCAATTAGAGGAAAGAACTGGAGGGAAGATCATTTACAGGAAAGGTGGAGTGATATACCTTTTCCGAGGCAGAAACTACAACTATAAAACTCGTCCTAAGTTTCCTCTAATGCTTTGGAGACCTATCACTCCTGTATATCCTAGACTGATTCAGCAGGCTCCTGAGGGTCTAACAGTAGAAGCAGCATCTGAGATGCGTAAGAAGGGACGGAATCTGATACCAATATGCAAGCTAGGGAAAAATGGTGTATACTCTGAATTGGTAAAAAATGTCAGAGAGGCCTTTGAAGAGTGTGAGCTAGTGCGAATAAATTGTCAGGGGATGAACGCGAGTGACTACCGAAAAATAGGTGGTAAACTAAAGGATCTTGTCCCTTGTGTGTTAATGTCATTTGAACTTGAGCACATTCTTTTGTGGAGAGGACGGGAATGGAAGTCTTCTCTTCCAAATCCAGAGAATGATCTCAAGGAAGTCAAAGAGTCTGATGTAGATTGTTCAACTTCAATTGCATCAACATCGTGTGCTTCTGAAGTTGTGGGTGCAGAGGGAAGTGAGGATCTCTCGCCATCACAATATGTTGGGCCACGTGCCACAGTTGATGGGGTTTCTACCGTTGGAGGAACCTCTGAAACTGAACCCATTTCAGATGTTGAAGGCTATATAAACAATGAATCAGAAGCTAAAATGACTGCTGATAACAGCTCAACCATACCAGACAATATCCACTATGCTGCTGATAAGTCAAAAACTATGCCCCATACATCTGAAATGGAGCCCATGTTGGCTAATGCAGGCTGTGATGATGAAGCATCACCAACCGCAGTAATGGGCTCTGAGGCCATCGCAATGCCTATTGGAAACTCTGAAACAAAGTTGGAGTCCATCACGGCAGGTTCTGGAAGCAACGAGAATCCAGAAGATGGTTCATTAGGCACAGAAATTCTTAGTGAGCCAGCAAAGCTGAGTGCACCTTGTATAGAGAATGTGCTGTTACTCTTGAATGAAGCTGTTGATAGTGGGAGTGCACTTATTTTAGACGAGTCATCGCTGAATGCCGACATTATTTATCAACGGGCAGTTGCCTTCGCACAGTCTGCTCCCCCTGGCCCCGTGTTTAAGCATCAACGACCCAAGAAGGTAGCAGTTCTAAAGAGAGTAAAGGTTGTGAAGCAAGATGCCGGGGATCCAGAAGTGAAAGAGATAACAGTTTCTGCGGAGAGAGAAAGTGAGAAGATTCAGAAGGGTAGTAAAGTTAAAAGAATAAGAGATTTTGGTGAAAATCTAGTAGATAACGTTGTGCCACAGGGAAGTTTGAGAGTTGATGAACTTGCTAAGCTATTAGCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000373 GO:0000375 GO:0000377 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008380 GO:0009987 GO:0010467 GO:0016070 GO:0034641 GO:0043170 GO:0044237 GO:0044238 GO:0046483 GO:0071704 GO:0090304 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CRS1_YhbY

Protein Analysis

775

Amino Acids

85.37

Weight (kDa)

8.72

Isoelectric Point (pI)

47.99

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CRS1_YhbY	PF01985	265 - 348	4.4e-21	CRS1 / YhbY (CRM) domain
CRS1_YhbY	PF01985	383 - 466	7.1e-16	CRS1 / YhbY (CRM) domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013996)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G20020
fragaria_vesca	FvH4_1g05780
malus_domestica	MD02G1059500.v1.1 MD15G1193500.v1.1
prunus_persica	Prupe.7G223600_v2.0.a1
pyrus_communis	pycom02g04850 pycom15g17320
rosa_chinensis	RchiOBHm_Chr2g0091431
rosa_laevigata	RLG00000016223
rosa_multiflora	Rmu_ssc0000333.1_g000008
rosa_roxburghii	Rroxscaffold_2G00150090
rosa_rugosa	Rorug02G0017800
rosa_samantha	Rh2AG062700 Rh2BG062000 Rh2CG063900 Rh2DG062300
rosa_wichuraiana	Rw2G005740

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1859
AccB7I	CCANNNNNTGG	1 cut(s)	1898
AccI	GTMKAC	3 cut(s)	453, 957, 1227
AccII	CGCG	3 cut(s)	38, 260, 1307
AclI	AACGTT	1 cut(s)	2271
AclWI	GGATC	6 cut(s)	751, 782, 1350, 1542, 2156, 2169
AcoI	YGGCCR	2 cut(s)	261, 614
AcsI	RAATTY	5 cut(s)	10, 137, 521, 665, 1917
AcuI	CTGAAG	1 cut(s)	1527
AcvI	CACGTG	1 cut(s)	1565
AfaI	GTAC	3 cut(s)	353, 530, 952
AfiI	CCNNNNNNNGG	5 cut(s)	94, 623, 640, 746, 1898
AflIII	ACRYGT	1 cut(s)	882
AjnI	CCWGG	3 cut(s)	334, 767, 2080
AloI	GAACNNNNNNTCC	4 cut(s)	89, 121, 566, 598
Alw21I	GWGCWC	3 cut(s)	1383, 1948, 2005
Alw26I	GTCTC	2 cut(s)	536, 1090
Alw44I	GTGCAC	2 cut(s)	1944, 2001
AlwI	GGATC	6 cut(s)	751, 782, 1350, 1542, 2156, 2169
AlwNI	CAGNNNCTG	3 cut(s)	1166, 1874, 2189
Ama87I	CYCGRG	3 cut(s)	52, 319, 430
AoxI	GGCC	9 cut(s)	156, 261, 596, 614, 630, 1257, 1559, 1815, 2083
ApaI	GGGCCC	3 cut(s)	160, 600, 634
ApaLI	GTGCAC	2 cut(s)	1944, 2001
ApeKI	GCWGC	3 cut(s)	682, 1160, 1709
ApoI	RAATTY	5 cut(s)	10, 137, 521, 665, 1917
ArsI	GACNNNNNNTTYG	4 cut(s)	1276, 1308, 1841, 1873
Asp700I	GAANNNNTTC	1 cut(s)	665
AspLEI	GCGC	5 cut(s)	7, 40, 150, 195, 915
AsuC2I	CCSGG	5 cut(s)	188, 572, 726, 741, 2158
AsuHPI	GGTGA	2 cut(s)	1782, 2264
AvaI	CYCGRG	3 cut(s)	52, 319, 430
AvaII	GGWCC	4 cut(s)	100, 610, 728, 749
AxyI	CCTNAGG	1 cut(s)	1140
BaeGI	GKGCMC	5 cut(s)	160, 600, 634, 1948, 2005
BamHI	GGATCC	1 cut(s)	2161
BanII	GRGCYC	8 cut(s)	59, 160, 326, 514, 600, 634, 1755, 1811
BarI	GAAGNNNNNNTAC	2 cut(s)	995, 1027
BbrPI	CACGTG	1 cut(s)	1565
BbsI	GAAGAC	1 cut(s)	1407
Bbv12I	GWGCWC	3 cut(s)	1383, 1948, 2005
BbvI	GCAGC	3 cut(s)	669, 1172, 1696
BceAI	ACGGC	3 cut(s)	292, 565, 1881
BcgI	CGANNNNNNTGC	2 cut(s)	1476, 1510
BciT130I	CCWGG	3 cut(s)	336, 769, 2082
BciVI	GTATCC	1 cut(s)	324
BcnI	CCSGG	5 cut(s)	188, 572, 726, 741, 2158
BcoDI	GTCTC	2 cut(s)	536, 1090
BfaI	CTAG	4 cut(s)	1119, 1211, 1277, 2261
BfoI	RGCGCY	3 cut(s)	8, 151, 916
BfuAI	ACCTGC	1 cut(s)	1859
BfuI	GTATCC	1 cut(s)	324
BglI	GCCNNNNNGGC	2 cut(s)	260, 595
BisI	GCNGC	7 cut(s)	255, 258, 261, 683, 692, 1161, 1710
BlpI	GCTNAGC	1 cut(s)	2310
BlsI	GCNGC	7 cut(s)	256, 259, 262, 684, 693, 1162, 1711
BmcAI	AGTACT	1 cut(s)	353
Bme1390I	CCNGG	8 cut(s)	188, 336, 572, 726, 741, 769, 2082, 2158
Bme18I	GGWCC	4 cut(s)	100, 610, 728, 749
BmeT110I	CYCGRG	3 cut(s)	52, 319, 430
BmrFI	CCNGG	8 cut(s)	188, 336, 572, 726, 741, 769, 2082, 2158
BmsI	GCATC	7 cut(s)	840, 1161, 1172, 1496, 1796, 2107, 2143
BpiI	GAAGAC	1 cut(s)	1407
BplI	GAGNNNNNCTC	2 cut(s)	1523, 1555
BpmI	CTGGAG	1 cut(s)	1017
Bpu1102I	GCTNAGC	1 cut(s)	2310
BpuEI	CTTGAG	2 cut(s)	1397, 1427
BpuMI	CCSGG	5 cut(s)	188, 572, 726, 741, 2158
BsaAI	YACGTR	1 cut(s)	1565
BsaI	GGTCTC	2 cut(s)	536, 1090
BsaJI	CCNNGG	8 cut(s)	258, 334, 431, 617, 768, 1042, 2080, 2157
BsaWI	WCCGGW	1 cut(s)	102
BsaXI	ACNNNNNCTCC	6 cut(s)	89, 119, 956, 986, 1742, 1772
Bsc4I	CCNNNNNNNGG	5 cut(s)	94, 623, 640, 746, 1898
Bse118I	RCCGGY	4 cut(s)	445, 612, 640, 712
Bse1I	ACTGG	2 cut(s)	911, 1000
Bse21I	CCTNAGG	1 cut(s)	1140
Bse3DI	GCAATG	2 cut(s)	635, 1830
BseBI	CCWGG	3 cut(s)	336, 769, 2082
BseDI	CCNNGG	8 cut(s)	258, 334, 431, 617, 768, 1042, 2080, 2157
BseGI	GGATG	1 cut(s)	1305
BseLI	CCNNNNNNNGG	5 cut(s)	94, 623, 640, 746, 1898
BseMI	GCAATG	2 cut(s)	635, 1830
BseMII	CTCAG	4 cut(s)	1131, 1158, 1803, 1931
BseNI	ACTGG	2 cut(s)	911, 1000
BseRI	GAGGAG	1 cut(s)	788
BseSI	GKGCMC	5 cut(s)	160, 600, 634, 1948, 2005
BseXI	GCAGC	3 cut(s)	669, 1172, 1696
BseYI	CCCAGC	1 cut(s)	159
BsgI	GTGCAG	1 cut(s)	1539
Bsh1236I	CGCG	3 cut(s)	38, 260, 1307
BshFI	GGCC	9 cut(s)	158, 263, 598, 616, 632, 1259, 1561, 1817, 2085
BsiHKAI	GWGCWC	3 cut(s)	1383, 1948, 2005
BsiHKCI	CYCGRG	3 cut(s)	52, 319, 430
BslFI	GGGAC	3 cut(s)	12, 1197, 1334
BslI	CCNNNNNNNGG	5 cut(s)	94, 623, 640, 746, 1898
BsmAI	GTCTC	2 cut(s)	536, 1090
BsmFI	GGGAC	3 cut(s)	12, 1197, 1334
BsmI	GAATGC	2 cut(s)	389, 2035
BsnI	GGCC	9 cut(s)	158, 263, 598, 616, 632, 1259, 1561, 1817, 2085
Bso31I	GGTCTC	2 cut(s)	536, 1090
BsoBI	CYCGRG	3 cut(s)	52, 319, 430
Bsp120I	GGGCCC	3 cut(s)	156, 596, 630
Bsp1407I	TGTACA	1 cut(s)	950
Bsp143I	GATC	7 cut(s)	743, 787, 1005, 1342, 1438, 1534, 2161
Bsp1720I	GCTNAGC	1 cut(s)	2310
Bsp19I	CCATGG	1 cut(s)	617
BspANI	GGCC	9 cut(s)	158, 263, 598, 616, 632, 1259, 1561, 1817, 2085
BspCNI	CTCAG	4 cut(s)	1132, 1159, 1804, 1932
BspFNI	CGCG	3 cut(s)	38, 260, 1307
BspMI	ACCTGC	1 cut(s)	1859
BspPI	GGATC	6 cut(s)	751, 782, 1350, 1542, 2156, 2169
BspTNI	GGTCTC	2 cut(s)	536, 1090
BsrDI	GCAATG	2 cut(s)	635, 1830
BsrFI	RCCGGY	4 cut(s)	445, 612, 640, 712
BsrGI	TGTACA	1 cut(s)	950
BsrI	ACTGG	2 cut(s)	911, 1000
BssAI	RCCGGY	4 cut(s)	445, 612, 640, 712
BssECI	CCNNGG	8 cut(s)	258, 334, 431, 617, 768, 1042, 2080, 2157
BssMI	GATC	7 cut(s)	743, 787, 1005, 1342, 1438, 1534, 2161
BssNAI	GTATAC	1 cut(s)	1228
BssT1I	CCWWGG	1 cut(s)	617
Bst1107I	GTATAC	1 cut(s)	1228
Bst2UI	CCWGG	3 cut(s)	336, 769, 2082
Bst4CI	ACNGT	7 cut(s)	799, 955, 1153, 1573, 1591, 2070, 2185
Bst6I	CTCTTC	2 cut(s)	1260, 1425
BstAPI	GCANNNNNTGC	1 cut(s)	2072
BstAUI	TGTACA	1 cut(s)	950
BstBAI	YACGTR	1 cut(s)	1565
BstC8I	GCNNGC	8 cut(s)	305, 642, 646, 977, 1134, 1208, 1771, 1932
BstDEI	CTNAG	8 cut(s)	753, 1074, 1140, 1167, 1812, 1922, 1940, 2310
BstDSI	CCRYGG	2 cut(s)	258, 617
BstF5I	GGATG	1 cut(s)	1305
BstFNI	CGCG	3 cut(s)	38, 260, 1307
BstH2I	RGCGCY	3 cut(s)	8, 151, 916
BstHHI	GCGC	5 cut(s)	7, 40, 150, 195, 915
BstKTI	GATC	7 cut(s)	746, 790, 1008, 1345, 1441, 1537, 2164
BstMAI	GTCTC	2 cut(s)	536, 1090
BstMBI	GATC	7 cut(s)	743, 787, 1005, 1342, 1438, 1534, 2161
BstNI	CCWGG	3 cut(s)	336, 769, 2082
BstNSI	RCATGY	2 cut(s)	886, 902
BstSCI	CCNGG	8 cut(s)	186, 334, 570, 724, 739, 767, 2080, 2156
BstSLI	GKGCMC	5 cut(s)	160, 600, 634, 1948, 2005
BstUI	CGCG	3 cut(s)	38, 260, 1307
BstV1I	GCAGC	3 cut(s)	669, 1172, 1696
BstV2I	GAAGAC	1 cut(s)	1407
BstX2I	RGATCY	4 cut(s)	787, 1342, 1534, 2161
BstXI	CCANNNNNNTGG	1 cut(s)	607
BstYI	RGATCY	4 cut(s)	787, 1342, 1534, 2161
BstZ17I	GTATAC	1 cut(s)	1228
Bsu36I	CCTNAGG	1 cut(s)	1140
BsuI	GTATCC	1 cut(s)	324
BsuRI	GGCC	9 cut(s)	158, 263, 598, 616, 632, 1259, 1561, 1817, 2085
BtgI	CCRYGG	2 cut(s)	258, 617
BtgZI	GCGATG	2 cut(s)	1804, 2007
BtsCI	GGATG	1 cut(s)	1305
BtsIMutI	CAGTG	2 cut(s)	240, 904
BveI	ACCTGC	1 cut(s)	1859
Cac8I	GCNNGC	8 cut(s)	305, 642, 646, 977, 1134, 1208, 1771, 1932
CaiI	CAGNNNCTG	3 cut(s)	1166, 1874, 2189
CfoI	GCGC	5 cut(s)	7, 40, 150, 195, 915
Cfr10I	RCCGGY	4 cut(s)	445, 612, 640, 712
Cfr42I	CCGCGG	1 cut(s)	261
Csp6I	GTAC	3 cut(s)	352, 529, 951
CspCI	CAANNNNNGTGG	2 cut(s)	199, 234
CviAII	CATG	6 cut(s)	618, 883, 899, 961, 1756, 2322
CviQI	GTAC	3 cut(s)	352, 529, 951
DdeI	CTNAG	8 cut(s)	753, 1074, 1140, 1167, 1812, 1922, 1940, 2310
DpnI	GATC	7 cut(s)	745, 789, 1007, 1344, 1440, 1536, 2163
DpnII	GATC	7 cut(s)	743, 787, 1005, 1342, 1438, 1534, 2161
EaeI	YGGCCR	2 cut(s)	261, 614
Eam1104I	CTCTTC	2 cut(s)	1260, 1425
EarI	CTCTTC	2 cut(s)	1260, 1425
EciI	GGCGGA	3 cut(s)	196, 383, 678
Eco130I	CCWWGG	1 cut(s)	617
Eco147I	AGGCCT	1 cut(s)	1259
Eco24I	GRGCYC	8 cut(s)	59, 160, 326, 514, 600, 634, 1755, 1811
Eco31I	GGTCTC	2 cut(s)	536, 1090
Eco47I	GGWCC	4 cut(s)	100, 610, 728, 749
Eco57I	CTGAAG	1 cut(s)	1527
Eco72I	CACGTG	1 cut(s)	1565
Eco81I	CCTNAGG	1 cut(s)	1140
Eco88I	CYCGRG	3 cut(s)	52, 319, 430
EcoO109I	RGGNCCY	3 cut(s)	156, 631, 749
EcoRI	GAATTC	2 cut(s)	521, 665
EcoRII	CCWGG	3 cut(s)	334, 767, 2080
EcoT14I	CCWWGG	1 cut(s)	617
EcoT38I	GRGCYC	8 cut(s)	59, 160, 326, 514, 600, 634, 1755, 1811
ErhI	CCWWGG	1 cut(s)	617
FaeI	CATG	6 cut(s)	621, 886, 902, 964, 1759, 2325
FaqI	GGGAC	3 cut(s)	12, 1197, 1334
FatI	CATG	6 cut(s)	617, 882, 898, 960, 1755, 2321
FauI	CCCGC	1 cut(s)	394
FblI	GTMKAC	3 cut(s)	453, 957, 1227
Fnu4HI	GCNGC	7 cut(s)	255, 258, 261, 683, 692, 1161, 1710
FokI	GGATG	1 cut(s)	1312
FriOI	GRGCYC	8 cut(s)	59, 160, 326, 514, 600, 634, 1755, 1811
Fsp4HI	GCNGC	7 cut(s)	255, 258, 261, 683, 692, 1161, 1710
FspBI	CTAG	4 cut(s)	1119, 1211, 1277, 2261
GlaI	GCGC	5 cut(s)	6, 39, 149, 194, 914
GluI	GCNGC	7 cut(s)	255, 258, 261, 683, 692, 1161, 1710
GsaI	CCCAGC	1 cut(s)	163
GsuI	CTGGAG	1 cut(s)	1017
HaeII	RGCGCY	3 cut(s)	8, 151, 916
HaeIII	GGCC	9 cut(s)	158, 263, 598, 616, 632, 1259, 1561, 1817, 2085
HhaI	GCGC	5 cut(s)	7, 40, 150, 195, 915
Hin1II	CATG	6 cut(s)	621, 886, 902, 964, 1759, 2325
Hin6I	GCGC	5 cut(s)	5, 38, 148, 193, 913
HinP1I	GCGC	5 cut(s)	5, 38, 148, 193, 913
HincII	GTYRAC	2 cut(s)	454, 958
HindII	GTYRAC	2 cut(s)	454, 958
HphI	GGTGA	2 cut(s)	1782, 2264
Hpy166II	GTNNAC	7 cut(s)	454, 585, 958, 1228, 1334, 1946, 2003
Hpy188III	TCNNGA	8 cut(s)	421, 430, 1139, 1430, 1875, 1892, 1975, 2165
Hpy8I	GTNNAC	7 cut(s)	454, 585, 958, 1228, 1334, 1946, 2003
Hpy99I	CGWCG	3 cut(s)	313, 485, 509
HpyAV	CCTTC	8 cut(s)	336, 655, 694, 1174, 1625, 2071, 2107, 2212
HpyCH4III	ACNGT	7 cut(s)	799, 955, 1153, 1573, 1591, 2070, 2185
HpyCH4IV	ACGT	3 cut(s)	498, 1564, 2271
HpyF3I	CTNAG	8 cut(s)	753, 1074, 1140, 1167, 1812, 1922, 1940, 2310
HpySE526I	ACGT	3 cut(s)	498, 1564, 2271
Hsp92II	CATG	6 cut(s)	621, 886, 902, 964, 1759, 2325
HspAI	GCGC	5 cut(s)	5, 38, 148, 193, 913
KroI	GCCGGC	1 cut(s)	640
KroNI	GCCGGC	1 cut(s)	642
KspI	CCGCGG	1 cut(s)	261
Kzo9I	GATC	7 cut(s)	743, 787, 1005, 1342, 1438, 1534, 2161
LmnI	GCTCC	6 cut(s)	190, 509, 573, 1141, 1750, 2080
Lsp1109I	GCAGC	3 cut(s)	669, 1172, 1696
LweI	GCATC	7 cut(s)	840, 1161, 1172, 1496, 1796, 2107, 2143
MaeI	CTAG	4 cut(s)	1119, 1211, 1277, 2261
MaeII	ACGT	3 cut(s)	498, 1564, 2271
MaeIII	GTNAC	2 cut(s)	1310, 1968
MalI	GATC	7 cut(s)	745, 789, 1007, 1344, 1440, 1536, 2163
MboI	GATC	7 cut(s)	743, 787, 1005, 1342, 1438, 1534, 2161
MboII	GAAGA	7 cut(s)	664, 1015, 1277, 1407, 1412, 1907, 2221
MfeI	CAATTG	1 cut(s)	1482
MflI	RGATCY	4 cut(s)	787, 1342, 1534, 2161
MlyI	GAGTC	8 cut(s)	212, 227, 408, 665, 868, 1466, 1865, 2027
MmeI	TCCRAC	5 cut(s)	116, 255, 867, 1573, 1833
MroNI	GCCGGC	1 cut(s)	640
MroXI	GAANNNNTTC	1 cut(s)	665
MseI	TTAA	8 cut(s)	564, 708, 810, 822, 933, 1361, 2094, 2232
MslI	CAYNNNNRTG	2 cut(s)	605, 1823
MspA1I	CMGCKG	2 cut(s)	260, 682
MspR9I	CCNGG	8 cut(s)	188, 336, 572, 726, 741, 769, 2082, 2158
MunI	CAATTG	1 cut(s)	1482
Mva1269I	GAATGC	2 cut(s)	389, 2035
MvaI	CCWGG	3 cut(s)	336, 769, 2082
MvnI	CGCG	3 cut(s)	38, 260, 1307
NaeI	GCCGGC	1 cut(s)	642
NciI	CCSGG	5 cut(s)	188, 572, 726, 741, 2158
NcoI	CCATGG	1 cut(s)	617
NdeII	GATC	7 cut(s)	743, 787, 1005, 1342, 1438, 1534, 2161
NgoMIV	GCCGGC	1 cut(s)	640
NlaIII	CATG	6 cut(s)	621, 886, 902, 964, 1759, 2325
NmeAIII	GCCGAG	2 cut(s)	157, 422
NmuCI	GTSAC	1 cut(s)	1310
NspI	RCATGY	2 cut(s)	886, 902
PaeR7I	CTCGAG	1 cut(s)	319
PceI	AGGCCT	1 cut(s)	1259
PciI	ACATGT	1 cut(s)	882
PcsI	WCGNNNNNNNCGW	4 cut(s)	129, 317, 419, 428
PctI	GAATGC	2 cut(s)	389, 2035
PdiI	GCCGGC	1 cut(s)	642
PdmI	GAANNNNTTC	1 cut(s)	665
PfeI	GAWTC	8 cut(s)	76, 198, 377, 1126, 1189, 1649, 1888, 2212
PflMI	CCANNNNNTGG	1 cut(s)	1898
PfoI	TCCNGGA	1 cut(s)	570
PkrI	GCNGC	7 cut(s)	256, 259, 262, 684, 693, 1162, 1711
PleI	GAGTC	8 cut(s)	211, 227, 407, 665, 868, 1465, 1864, 2026
PmaCI	CACGTG	1 cut(s)	1565
PmlI	CACGTG	1 cut(s)	1565
PpsI	GAGTC	8 cut(s)	211, 227, 407, 665, 868, 1465, 1864, 2026
Ppu21I	YACGTR	1 cut(s)	1565
PpuMI	RGGWCCY	1 cut(s)	749
PscI	ACATGT	1 cut(s)	882
Psp1406I	AACGTT	1 cut(s)	2271
Psp5II	RGGWCCY	1 cut(s)	749
Psp6I	CCWGG	3 cut(s)	334, 767, 2080
PspCI	CACGTG	1 cut(s)	1565
PspFI	CCCAGC	1 cut(s)	159
PspGI	CCWGG	3 cut(s)	334, 767, 2080
PspOMI	GGGCCC	3 cut(s)	156, 596, 630
PspPPI	RGGWCCY	1 cut(s)	749
PspXI	VCTCGAGB	1 cut(s)	319
PstNI	CAGNNNCTG	3 cut(s)	1166, 1874, 2189
PsuI	RGATCY	4 cut(s)	787, 1342, 1534, 2161
PvuII	CAGCTG	1 cut(s)	682
RsaI	GTAC	3 cut(s)	353, 530, 952
RsaNI	GTAC	3 cut(s)	352, 529, 951
RseI	CAYNNNNRTG	2 cut(s)	605, 1823
SacII	CCGCGG	1 cut(s)	261
SalI	GTCGAC	2 cut(s)	452, 956
SaqAI	TTAA	8 cut(s)	564, 708, 810, 822, 933, 1361, 2094, 2232
SatI	GCNGC	7 cut(s)	255, 258, 261, 683, 692, 1161, 1710
Sau3AI	GATC	7 cut(s)	743, 787, 1005, 1342, 1438, 1534, 2161
ScaI	AGTACT	1 cut(s)	353
SchI	GAGTC	8 cut(s)	212, 227, 408, 665, 868, 1466, 1865, 2027
ScrFI	CCNGG	8 cut(s)	188, 336, 572, 726, 741, 769, 2082, 2158
SfaNI	GCATC	7 cut(s)	840, 1161, 1172, 1496, 1796, 2107, 2143
Sfr274I	CTCGAG	1 cut(s)	319
Sfr303I	CCGCGG	1 cut(s)	261
SgrBI	CCGCGG	1 cut(s)	261
SinI	GGWCC	4 cut(s)	100, 610, 728, 749
SlaI	CTCGAG	1 cut(s)	319
SmiMI	CAYNNNNRTG	2 cut(s)	605, 1823
SmlI	CTYRAG	3 cut(s)	319, 1376, 1442
SmoI	CTYRAG	3 cut(s)	319, 1376, 1442
SseBI	AGGCCT	1 cut(s)	1259
SspMI	CTAG	4 cut(s)	1119, 1211, 1277, 2261
StuI	AGGCCT	1 cut(s)	1259
StyD4I	CCNGG	8 cut(s)	186, 334, 570, 724, 739, 767, 2080, 2156
StyI	CCWWGG	1 cut(s)	617
TaaI	ACNGT	7 cut(s)	799, 955, 1153, 1573, 1591, 2070, 2185
TaiI	ACGT	3 cut(s)	501, 1567, 2274
TaqI	TCGA	8 cut(s)	171, 201, 320, 422, 453, 504, 669, 957
TatI	WGTACW	3 cut(s)	351, 528, 950
TauI	GCSGC	4 cut(s)	257, 260, 263, 694
TfiI	GAWTC	8 cut(s)	76, 198, 377, 1126, 1189, 1649, 1888, 2212
Tru1I	TTAA	8 cut(s)	564, 708, 810, 822, 933, 1361, 2094, 2232
Tru9I	TTAA	8 cut(s)	564, 708, 810, 822, 933, 1361, 2094, 2232
TscAI	CASTG	2 cut(s)	247, 911
TseFI	GTSAC	1 cut(s)	1310
TseI	GCWGC	3 cut(s)	682, 1160, 1709
Tsp45I	GTSAC	1 cut(s)	1310
TspDTI	ATGAA	6 cut(s)	1316, 1662, 1797, 1893, 1995, 2316
TspGWI	ACGGA	4 cut(s)	110, 522, 720, 1201
TspRI	CASTG	2 cut(s)	247, 911
Van91I	CCANNNNNTGG	1 cut(s)	1898
VneI	GTGCAC	2 cut(s)	1944, 2001
VpaK11BI	GGWCC	4 cut(s)	100, 610, 728, 749
XapI	RAATTY	5 cut(s)	10, 137, 521, 665, 1917
XceI	RCATGY	2 cut(s)	886, 902
XhoI	CTCGAG	1 cut(s)	319
XmiI	GTMKAC	3 cut(s)	453, 957, 1227
XmnI	GAANNNNTTC	1 cut(s)	665
XspI	CTAG	4 cut(s)	1119, 1211, 1277, 2261
ZrmI	AGTACT	1 cut(s)	353

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.