RchiOBHm_Chr2g0091431

CRS2-associated factor 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0013996
Physical Location & Seq
Reverse (-)
5089169 .. 5092719
3551 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ46661

Sequence Viewer

Length: 2481 bp
ATGGCGCTAAATTTGCCGACTTCGTGCCCCATTTTCGCGCCACCGGTGAACCCGAACCCGGCCCACAACCCACTCCAGACCCGCCCGCCCACGGAGGTCCGATTCGCGAGGTGGAACAACGCCAACGCCGAGAAATTCAACCAGCGCCGCCGCGCCCAGCAAGAAATCGAGGACGACGTCCGCCGCGAGCGCCGCTTCGACTCCGCCACCAGAATCGCCACCGTCGCCGCCGCCGACTCCTCCACCTCCTCCGACGCCGAACCAACATTCAAATCCATAGGCACGCCTTCGTCTCCCTCAAGCCCTTCAATTCCCGGCAGAAAATCCAAGTACTCCAAGAACCCTAGCCCTAGCTCGCACCCGGCGTTTCGGCGAGTCATCAGACCCACGAAGCTCTCGAGTATCGCCAGAGAGAAGCCGGAAATCGACCGGAAAGCTAACATCTCCGTCGGCGACGACGGGCTTTCGTACGTCATCGACGGCGCGCCGTTTGAGTTCAAGTACAGCTACACCGAGACCCCGAAGCAGAAGCCCATTAAGCTCCGGGAGCCGCCCTACGCGCCGTTCGGCCCCACCACGATGGGCCGGCCGTGGACGGGTCGGGCCCCATTGCCTCCGAGCAAGAAGAAACTGAAGGAGTTCGATTCGTTCCAGCTACCGCCGCCGCATAAGAAAGGGGTTAAGCCGGTGCAGTCGCCGGGTCCGTATTTACCCGGGTCGGGTCCCAAGTATGTCAAGTCCAGGGAGGAGATATTGGGTGAGCCGTTGACCGATCAGGAAGTCAAAGACTTGGTTAATAGCTGTGTTAAGACCAGAAGGCAACTCAATATGGGTAGAGATGGATTGACACACAACATGCTGGATAATATTCATGCTCATTGGAAGCGGCGGAGAGTGTGCAAGATAAAATGTAAAGGGGTGTGTACAGTCGACATGGAAAATGTGTGCCAGCAATTAGAGGAAAGAACTGGAGGGAAAATCATTTATAGAAGAGGTGGGGTGATTTTTCTTTTCCGGGGGAGAAACTACAACTATAAAACTCGGCCGCGGTTTCCTCTCATGCTTTGGAGACCTATCACTCCTGTGTATCCTAGATTGATTCAGAGGGCTCCTGAGGGACTAACAGTTGAAGAAGCAACTGAAATGCGCAAGAAGGGGAGGAATCTGATACCAATACGCAAGCTAGGAAAAAATGGAGTATACTCTGACCTGGTAGACAATGTCAGAGAGGCCTTTGAAGAGTGTGAACTAGTGCGAATAGATTGTCAAGGGATGAATGGGAGTGACTACCGTAAAATAGGTGCTAAACTAAAGGATCTTGTCCCTTGTGTGCTAATTTCCTTTGAACGCGAGAACATTCTTGTGTGGAGAGGACAGGAATGGAAGTCTTCTTTCCTAAATCCGGAGAGTAATCTTAAGGAAGTCAAAGAATCTGATGTTGATGATTCATCTTCTATTGCACCTTCCTTGGAAGGTGAAGAAGCATTGACGTTATGTGCTTTTACAGCTTCAGTCAATGATGAAAATCCTCAAATGATCGACACTAGTATCACCTCTTCAAGTTCTGAAGTTGTGGGTGCTGAGGGAACTGAGGATCCATCCCCTTCACCATATATTGAGCCATGTGCCACAATTGATACAGTTTCCACTGTGGGAGGCACTTATGAAACTGTAACCATCTCGGACATCAAAGGCTTTAGAAATGATGAAGCAGAATTGGAGATGAAGGCCTATAGCAGTTCAACCATACCACAGGATACCAGGGATGCTCATGATGAGGCAGAAACGATTTCCATCTCAGACATCAAGGGCTTTAGAAATAATGAAGCAGAATTGGAAATGAAGGCCTATATCAGTTCCTGCGGCAATGCTGATGATGAGACCAAAACAATTTCCAGTACATCTGGAACTGAAAACATATTGGATAGTACGTGCCATACTGATGAAGCATCAGCAACTACATCAGTGGGGACCGGGACCATGCCAGTGACTGTTGAAAGCACTGAAACGAAGTTGGTTGCCCCTGGTAGTAATGAGACTCCACAAGATGCTTGTATAGACTCGCAAAATCTCAATGAGCAAGCAAGGTTGAGTGCAGCCTGTAAAGAGAAGGTGCTATCACTCTTGAACGAAGCTGTTGGGAGTGGGAGTGCACTTATTTTAGACGATTCGTCGTTGGATGCTGACATCATATATCAGAGGGCAGTTGCTCTCGCCAATTCTGCTCCACCTGGGCCAGTGTTTAAGCATCGACCTAGGAGAGTTGCAGCTCAAATGAGTAAGAAGCTAGTAGTAAGAAAACAGAAGCAAGAAGCCACTGAGTTGGAAGTGAAAGAGATAACAGTTTATGCGAAGAAGGGAAGTGAGAAGAAGGATTCCAAAGTTCACAGAACAAAAACAAGAGATTTTGGTGAACCTCTAGATAGCATTGTACCACAGGGAAGCCTGAGAGTTGATGAACTTGCTAAGCTATTAGCATGA

Protein Analysis

826

Amino Acids

91.39

Weight (kDa)

8.18

Isoelectric Point (pI)

52.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRS1_YhbY PF01985 256 - 339 1.1e-20 CRS1 / YhbY (CRM) domain
CRS1_YhbY PF01985 374 - 457 1.4e-16 CRS1 / YhbY (CRM) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013996)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G20020
fragaria_vesca FvH4_1g05780
malus_domestica MD02G1059500.v1.1 MD15G1193500.v1.1
prunus_persica Prupe.7G223600_v2.0.a1
pyrus_communis pycom02g04850 pycom15g17320
rosa_chinensis RchiOBHm_Chr2g0091431
rosa_laevigata RLG00000016223
rosa_multiflora Rmu_ssc0000333.1_g000008
rosa_roxburghii Rroxscaffold_2G00150090
rosa_rugosa Rorug02G0017800
rosa_samantha Rh2AG062700 Rh2BG062000 Rh2CG063900 Rh2DG062300
rosa_wichuraiana Rw2G005740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 180
Acc16I TGCGCA 1 cut(s) 1148
AccI GTMKAC 3 cut(s) 930, 1200, 1215
AccII CGCG 8 cut(s) 38, 107, 153, 186, 485, 560, 1048, 1350
AccIII TCCGGA 1 cut(s) 1402
AclWI GGATC 3 cut(s) 1323, 1589, 1602
AcoI YGGCCR 2 cut(s) 587, 1043
AcsI RAATTY 2 cut(s) 10, 134
AcuI CTGAAG 3 cut(s) 653, 1494, 1587
AcyI GRCGYC 2 cut(s) 177, 255
AfaI GTAC 7 cut(s) 332, 470, 503, 925, 1900, 1930, 2433
AfiI CCNNNNNNNGG 5 cut(s) 58, 91, 596, 719, 1402
AflII CTTAAG 1 cut(s) 1415
AgeI ACCGGT 1 cut(s) 43
AhdI GACNNNNNGTC 1 cut(s) 2170
AhlI ACTAGT 2 cut(s) 1249, 1544
AjnI CCWGG 5 cut(s) 740, 1209, 1760, 2023, 2230
AleI CACNNNNGTG 1 cut(s) 1082
AloI GAACNNNNNNTCC 2 cut(s) 1579, 1611
Alw21I GWGCWC 1 cut(s) 2155
Alw26I GTCTC 5 cut(s) 297, 509, 1063, 1874, 2030
Alw44I GTGCAC 1 cut(s) 2151
AlwI GGATC 3 cut(s) 1323, 1589, 1602
AlwNI CAGNNNCTG 2 cut(s) 1860, 1991
Ama87I CYCGRG 2 cut(s) 397, 713
Aor13HI TCCGGA 1 cut(s) 1402
ApaI GGGCCC 1 cut(s) 607
ApaLI GTGCAC 1 cut(s) 2151
ApeKI GCWGC 2 cut(s) 2096, 2267
ApoI RAATTY 2 cut(s) 10, 134
ArsI GACNNNNNNTTYG 2 cut(s) 1249, 1281
AscI GGCGCGCC 1 cut(s) 483
AsiGI ACCGGT 1 cut(s) 43
Asp700I GAANNNNTTC 1 cut(s) 638
AspA2I CCTAGG 1 cut(s) 2255
AspLEI GCGC 9 cut(s) 7, 40, 147, 155, 192, 485, 487, 562, 1149
AsuC2I CCSGG 9 cut(s) 59, 315, 362, 545, 699, 714, 715, 1016, 1975
AsuHPI GGTGA 7 cut(s) 58, 770, 1012, 1487, 1543, 1599, 2423
AvaI CYCGRG 2 cut(s) 397, 713
AvaII GGWCC 5 cut(s) 97, 701, 722, 1971, 1977
AvrII CCTAGG 1 cut(s) 2255
AxyI CCTNAGG 1 cut(s) 1113
BaeGI GKGCMC 3 cut(s) 29, 607, 2155
BaeI ACNNNNGTAYC 2 cut(s) 1531, 1564
BamHI GGATCC 1 cut(s) 1594
BanII GRGCYC 2 cut(s) 607, 1111
BarI GAAGNNNNNNTAC 2 cut(s) 1540, 1572
BbsI GAAGAC 1 cut(s) 1380
Bbv12I GWGCWC 1 cut(s) 2155
BbvCI CCTCAGC 1 cut(s) 1581
BbvI GCAGC 2 cut(s) 2108, 2279
BccI CCATC 5 cut(s) 574, 833, 1606, 1685, 1802
BceAI ACGGC 5 cut(s) 472, 496, 547, 574, 748
BciT130I CCWGG 5 cut(s) 742, 1211, 1762, 2025, 2232
BciVI GTATCC 2 cut(s) 1098, 1750
BcnI CCSGG 9 cut(s) 59, 315, 362, 545, 699, 714, 715, 1016, 1975
BcoDI GTCTC 5 cut(s) 297, 509, 1063, 1874, 2030
BcuI ACTAGT 2 cut(s) 1249, 1544
BfaI CTAG 9 cut(s) 345, 351, 1092, 1184, 1250, 1545, 2256, 2288, 2420
BfmI CTRYAG 1 cut(s) 1732
BfoI RGCGCY 3 cut(s) 8, 148, 193
BfrI CTTAAG 1 cut(s) 1415
BfuI GTATCC 2 cut(s) 1098, 1750
BlnI CCTAGG 1 cut(s) 2255
BlpI GCTNAGC 1 cut(s) 2466
BmcAI AGTACT 1 cut(s) 332
Bme18I GGWCC 5 cut(s) 97, 701, 722, 1971, 1977
BmeRI GACNNNNNGTC 1 cut(s) 2170
BmeT110I CYCGRG 2 cut(s) 397, 713
BmsI GCATC 5 cut(s) 1756, 1958, 2038, 2170, 2257
BpiI GAAGAC 1 cut(s) 1380
BpmI CTGGAG 2 cut(s) 59, 990
Bpu10I CCTNAGC 1 cut(s) 1581
Bpu1102I GCTNAGC 1 cut(s) 2466
BpuEI CTTGAG 1 cut(s) 283
BpuMI CCSGG 9 cut(s) 59, 315, 362, 545, 699, 714, 715, 1016, 1975
BsaAI YACGTR 1 cut(s) 1932
BsaBI GATNNNNATC 2 cut(s) 1524, 1793
BsaHI GRCGYC 2 cut(s) 177, 255
BsaI GGTCTC 3 cut(s) 509, 1063, 1874
BsaWI WCCGGW 3 cut(s) 43, 429, 1402
BsaXI ACNNNNNCTCC 2 cut(s) 2134, 2164
Bsc4I CCNNNNNNNGG 5 cut(s) 58, 91, 596, 719, 1402
Bse118I RCCGGY 3 cut(s) 43, 585, 685
Bse1I ACTGG 4 cut(s) 973, 1896, 1985, 2237
Bse21I CCTNAGG 1 cut(s) 1113
Bse3DI GCAATG 2 cut(s) 608, 1873
Bse8I GATNNNNATC 2 cut(s) 1524, 1793
BseAI TCCGGA 1 cut(s) 1402
BseBI CCWGG 5 cut(s) 742, 1211, 1762, 2025, 2232
BseGI GGATG 4 cut(s) 1278, 1598, 1771, 2185
BseJI GATNNNNATC 2 cut(s) 1524, 1793
BseLI CCNNNNNNNGG 5 cut(s) 58, 91, 596, 719, 1402
BseMI GCAATG 2 cut(s) 608, 1873
BseMII CTCAG 6 cut(s) 1104, 1572, 1581, 1812, 2310, 2438
BseNI ACTGG 4 cut(s) 973, 1896, 1985, 2237
BsePI GCGCGC 1 cut(s) 483
BseRI GAGGAG 3 cut(s) 229, 238, 761
BseSI GKGCMC 3 cut(s) 29, 607, 2155
BseX3I CGGCCG 2 cut(s) 587, 1043
BseXI GCAGC 2 cut(s) 2108, 2279
BseYI CCCAGC 1 cut(s) 156
BsgI GTGCAG 2 cut(s) 710, 2115
Bsh1236I CGCG 8 cut(s) 38, 107, 153, 186, 485, 560, 1048, 1350
Bsh1285I CGRYCG 3 cut(s) 430, 590, 1046
BshTI ACCGGT 1 cut(s) 43
BsiEI CGRYCG 3 cut(s) 430, 590, 1046
BsiHKAI GWGCWC 1 cut(s) 2155
BsiHKCI CYCGRG 2 cut(s) 397, 713
BsiWI CGTACG 1 cut(s) 468
BslFI GGGAC 5 cut(s) 708, 1131, 1307, 1984, 1990
BslI CCNNNNNNNGG 5 cut(s) 58, 91, 596, 719, 1402
BsmAI GTCTC 5 cut(s) 297, 509, 1063, 1874, 2030
BsmBI CGTCTC 1 cut(s) 297
BsmFI GGGAC 5 cut(s) 708, 1131, 1307, 1984, 1990
Bso31I GGTCTC 3 cut(s) 509, 1063, 1874
BsoBI CYCGRG 2 cut(s) 397, 713
Bsp120I GGGCCC 1 cut(s) 603
Bsp1286I GDGCHC 4 cut(s) 29, 607, 1111, 2155
Bsp13I TCCGGA 1 cut(s) 1402
Bsp1407I TGTACA 1 cut(s) 923
Bsp143I GATC 4 cut(s) 772, 1315, 1536, 1594
Bsp1720I GCTNAGC 1 cut(s) 2466
Bsp68I TCGCGA 1 cut(s) 107
BspCNI CTCAG 6 cut(s) 1105, 1573, 1582, 1811, 2311, 2439
BspEI TCCGGA 1 cut(s) 1402
BspFNI CGCG 8 cut(s) 38, 107, 153, 186, 485, 560, 1048, 1350
BspHI TCATGA 1 cut(s) 1771
BspPI GGATC 3 cut(s) 1323, 1589, 1602
BspTI CTTAAG 1 cut(s) 1415
BspTNI GGTCTC 3 cut(s) 509, 1063, 1874
BsrDI GCAATG 2 cut(s) 608, 1873
BsrFI RCCGGY 3 cut(s) 43, 585, 685
BsrGI TGTACA 1 cut(s) 923
BsrI ACTGG 4 cut(s) 973, 1896, 1985, 2237
BssAI RCCGGY 3 cut(s) 43, 585, 685
BssHII GCGCGC 1 cut(s) 483
BssMI GATC 4 cut(s) 772, 1315, 1536, 1594
BssNAI GTATAC 1 cut(s) 1201
BssNI GRCGYC 2 cut(s) 177, 255
BssT1I CCWWGG 2 cut(s) 1467, 2255
Bst1107I GTATAC 1 cut(s) 1201
Bst2UI CCWGG 5 cut(s) 742, 1211, 1762, 2025, 2232
Bst4CI ACNGT 9 cut(s) 223, 928, 1126, 1292, 1642, 1651, 1672, 1993, 2344
Bst6I CTCTTC 3 cut(s) 985, 1233, 1561
BstACI GRCGYC 2 cut(s) 177, 255
BstAFI CTTAAG 1 cut(s) 1415
BstAUI TGTACA 1 cut(s) 923
BstBAI YACGTR 1 cut(s) 1932
BstC8I GCNNGC 9 cut(s) 86, 188, 284, 356, 485, 587, 950, 1181, 2082
BstDEI CTNAG 7 cut(s) 1113, 1581, 1590, 1798, 2319, 2447, 2466
BstDSI CCRYGG 3 cut(s) 90, 590, 1046
BstF5I GGATG 4 cut(s) 1278, 1598, 1771, 2185
BstFNI CGCG 8 cut(s) 38, 107, 153, 186, 485, 560, 1048, 1350
BstH2I RGCGCY 3 cut(s) 8, 148, 193
BstHHI GCGC 9 cut(s) 7, 40, 147, 155, 192, 485, 487, 562, 1149
BstKTI GATC 4 cut(s) 775, 1318, 1539, 1597
BstMAI GTCTC 5 cut(s) 297, 509, 1063, 1874, 2030
BstMBI GATC 4 cut(s) 772, 1315, 1536, 1594
BstMCI CGRYCG 3 cut(s) 430, 590, 1046
BstNI CCWGG 5 cut(s) 742, 1211, 1762, 2025, 2232
BstNSI RCATGY 1 cut(s) 859
BstSFI CTRYAG 1 cut(s) 1732
BstSLI GKGCMC 3 cut(s) 29, 607, 2155
BstUI CGCG 8 cut(s) 38, 107, 153, 186, 485, 560, 1048, 1350
BstV1I GCAGC 2 cut(s) 2108, 2279
BstV2I GAAGAC 1 cut(s) 1380
BstX2I RGATCY 2 cut(s) 1315, 1594
BstXI CCANNNNNNTGG 2 cut(s) 580, 2323
BstYI RGATCY 2 cut(s) 1315, 1594
BstZ17I GTATAC 1 cut(s) 1201
BstZI CGGCCG 2 cut(s) 587, 1043
Bsu36I CCTNAGG 1 cut(s) 1113
BsuI GTATCC 2 cut(s) 1098, 1750
BtgI CCRYGG 3 cut(s) 90, 590, 1046
BtsCI GGATG 4 cut(s) 1278, 1598, 1771, 2185
BtsIMutI CAGTG 6 cut(s) 1647, 1971, 1992, 2001, 2244, 2316
BtuMI TCGCGA 1 cut(s) 107
Cac8I GCNNGC 9 cut(s) 86, 188, 284, 356, 485, 587, 950, 1181, 2082
CaiI CAGNNNCTG 2 cut(s) 1860, 1991
CciI TCATGA 1 cut(s) 1771
CfoI GCGC 9 cut(s) 7, 40, 147, 155, 192, 485, 487, 562, 1149
Cfr10I RCCGGY 3 cut(s) 43, 585, 685
Cfr42I CCGCGG 1 cut(s) 1049
Cfr9I CCCGGG 1 cut(s) 713
CseI GACGC 1 cut(s) 263
CsiI ACCWGGT 1 cut(s) 1209
Csp6I GTAC 7 cut(s) 331, 469, 502, 924, 1899, 1929, 2432
CspAI ACCGGT 1 cut(s) 43
CviAII CATG 8 cut(s) 856, 872, 934, 1060, 1623, 1772, 1981, 2478
CviQI GTAC 7 cut(s) 331, 469, 502, 924, 1899, 1929, 2432
DdeI CTNAG 7 cut(s) 1113, 1581, 1590, 1798, 2319, 2447, 2466
DpnI GATC 4 cut(s) 774, 1317, 1538, 1596
DpnII GATC 4 cut(s) 772, 1315, 1536, 1594
DriI GACNNNNNGTC 1 cut(s) 2170
EaeI YGGCCR 2 cut(s) 587, 1043
EagI CGGCCG 2 cut(s) 587, 1043
Eam1104I CTCTTC 3 cut(s) 985, 1233, 1561
Eam1105I GACNNNNNGTC 1 cut(s) 2170
EarI CTCTTC 3 cut(s) 985, 1233, 1561
EciI GGCGGA 3 cut(s) 170, 193, 904
EclXI CGGCCG 2 cut(s) 587, 1043
Eco130I CCWWGG 2 cut(s) 1467, 2255
Eco147I AGGCCT 3 cut(s) 1232, 1730, 1847
Eco24I GRGCYC 2 cut(s) 607, 1111
Eco31I GGTCTC 3 cut(s) 509, 1063, 1874
Eco47I GGWCC 5 cut(s) 97, 701, 722, 1971, 1977
Eco52I CGGCCG 2 cut(s) 587, 1043
Eco57I CTGAAG 3 cut(s) 653, 1494, 1587
Eco81I CCTNAGG 1 cut(s) 1113
Eco88I CYCGRG 2 cut(s) 397, 713
EcoO109I RGGNCCY 2 cut(s) 604, 722
EcoRII CCWGG 5 cut(s) 740, 1209, 1760, 2023, 2230
EcoT14I CCWWGG 2 cut(s) 1467, 2255
EcoT38I GRGCYC 2 cut(s) 607, 1111
ErhI CCWWGG 2 cut(s) 1467, 2255
Esp3I CGTCTC 1 cut(s) 297
FaeI CATG 8 cut(s) 859, 875, 937, 1063, 1626, 1775, 1984, 2481
FaqI GGGAC 5 cut(s) 708, 1131, 1307, 1984, 1990
FatI CATG 8 cut(s) 855, 871, 933, 1059, 1622, 1771, 1980, 2477
FauI CCCGC 2 cut(s) 89, 93
FblI GTMKAC 3 cut(s) 930, 1200, 1215
FokI GGATG 4 cut(s) 1285, 1585, 1778, 2192
FriOI GRGCYC 2 cut(s) 607, 1111
FseI GGCCGGCC 1 cut(s) 589
FspBI CTAG 9 cut(s) 345, 351, 1092, 1184, 1250, 1545, 2256, 2288, 2420
FspI TGCGCA 1 cut(s) 1148
GlaI GCGC 9 cut(s) 6, 39, 146, 154, 191, 484, 486, 561, 1148
GsaI CCCAGC 1 cut(s) 160
GsuI CTGGAG 2 cut(s) 59, 990
HaeII RGCGCY 3 cut(s) 8, 148, 193
HgaI GACGC 1 cut(s) 263
HhaI GCGC 9 cut(s) 7, 40, 147, 155, 192, 485, 487, 562, 1149
Hin1I GRCGYC 2 cut(s) 177, 255
Hin1II CATG 8 cut(s) 859, 875, 937, 1063, 1626, 1775, 1984, 2481
Hin6I GCGC 9 cut(s) 5, 38, 145, 153, 190, 483, 485, 560, 1147
HinP1I GCGC 9 cut(s) 5, 38, 145, 153, 190, 483, 485, 560, 1147
HincII GTYRAC 2 cut(s) 768, 931
HindII GTYRAC 2 cut(s) 768, 931
HphI GGTGA 7 cut(s) 58, 770, 1012, 1487, 1543, 1599, 2423
Hpy99I CGWCG 8 cut(s) 179, 227, 257, 452, 458, 461, 482, 2176
HpyCH4III ACNGT 9 cut(s) 223, 928, 1126, 1292, 1642, 1651, 1672, 1993, 2344
HpyCH4IV ACGT 4 cut(s) 177, 471, 1490, 1931
HpyCH4V TGCA 6 cut(s) 691, 900, 1460, 2096, 2153, 2267
HpyF3I CTNAG 7 cut(s) 1113, 1581, 1590, 1798, 2319, 2447, 2466
HpySE526I ACGT 4 cut(s) 177, 471, 1490, 1931
Hsp92I GRCGYC 2 cut(s) 177, 255
Hsp92II CATG 8 cut(s) 859, 875, 937, 1063, 1626, 1775, 1984, 2481
HspAI GCGC 9 cut(s) 5, 38, 145, 153, 190, 483, 485, 560, 1147
KflI GGGWCCC 1 cut(s) 722
Kpn2I TCCGGA 1 cut(s) 1402
KroI GCCGGC 1 cut(s) 585
KroNI GCCGGC 1 cut(s) 587
KspI CCGCGG 1 cut(s) 1049
Kzo9I GATC 4 cut(s) 772, 1315, 1536, 1594
LmnI GCTCC 4 cut(s) 546, 547, 1114, 2230
Lsp1109I GCAGC 2 cut(s) 2108, 2279
LweI GCATC 5 cut(s) 1756, 1958, 2038, 2170, 2257
MabI ACCWGGT 1 cut(s) 1209
MaeI CTAG 9 cut(s) 345, 351, 1092, 1184, 1250, 1545, 2256, 2288, 2420
MaeII ACGT 4 cut(s) 177, 471, 1490, 1931
MaeIII GTNAC 3 cut(s) 1283, 1672, 1987
MalI GATC 4 cut(s) 774, 1317, 1538, 1596
MboI GATC 4 cut(s) 772, 1315, 1536, 1594
MfeI CAATTG 1 cut(s) 1632
MflI RGATCY 2 cut(s) 1315, 1594
MhlI GDGCHC 4 cut(s) 29, 607, 1111, 2155
MlyI GAGTC 5 cut(s) 194, 230, 384, 2032, 2054
MmeI TCCRAC 3 cut(s) 276, 2157, 2304
MroI TCCGGA 1 cut(s) 1402
MroNI GCCGGC 1 cut(s) 585
MroXI GAANNNNTTC 1 cut(s) 638
MseI TTAA 6 cut(s) 537, 681, 795, 807, 1416, 2244
MslI CAYNNNNRTG 5 cut(s) 578, 1082, 1361, 1941, 1985
MspA1I CMGCKG 1 cut(s) 1048
MspCI CTTAAG 1 cut(s) 1415
MunI CAATTG 1 cut(s) 1632
MvaI CCWGG 5 cut(s) 742, 1211, 1762, 2025, 2232
MvnI CGCG 8 cut(s) 38, 107, 153, 186, 485, 560, 1048, 1350
NaeI GCCGGC 1 cut(s) 587
NciI CCSGG 9 cut(s) 59, 315, 362, 545, 699, 714, 715, 1016, 1975
NdeII GATC 4 cut(s) 772, 1315, 1536, 1594
NgoMIV GCCGGC 1 cut(s) 585
NlaIII CATG 8 cut(s) 859, 875, 937, 1063, 1626, 1775, 1984, 2481
NmeAIII GCCGAG 2 cut(s) 154, 1021
NmuCI GTSAC 2 cut(s) 1283, 1987
NruI TCGCGA 1 cut(s) 107
NsbI TGCGCA 1 cut(s) 1148
NspI RCATGY 1 cut(s) 859
OliI CACNNNNGTG 1 cut(s) 1082
PaeR7I CTCGAG 1 cut(s) 397
PagI TCATGA 1 cut(s) 1771
PalAI GGCGCGCC 1 cut(s) 483
PauI GCGCGC 1 cut(s) 483
PceI AGGCCT 3 cut(s) 1232, 1730, 1847
PcsI WCGNNNNNNNCGW 7 cut(s) 126, 174, 183, 231, 362, 395, 701
PdiI GCCGGC 1 cut(s) 587
PdmI GAANNNNTTC 1 cut(s) 638
PfeI GAWTC 9 cut(s) 102, 213, 644, 1099, 1162, 1430, 1445, 2168, 2375
Pfl23II CGTACG 1 cut(s) 468
PflFI GACNNNGTC 2 cut(s) 176, 1220
PfoI TCCNGGA 1 cut(s) 543
PinAI ACCGGT 1 cut(s) 43
PleI GAGTC 5 cut(s) 194, 230, 383, 2032, 2054
PpsI GAGTC 5 cut(s) 194, 230, 383, 2032, 2054
Ppu21I YACGTR 1 cut(s) 1932
PpuMI RGGWCCY 1 cut(s) 722
Psp5II RGGWCCY 1 cut(s) 722
Psp6I CCWGG 5 cut(s) 740, 1209, 1760, 2023, 2230
PspFI CCCAGC 1 cut(s) 156
PspGI CCWGG 5 cut(s) 740, 1209, 1760, 2023, 2230
PspLI CGTACG 1 cut(s) 468
PspOMI GGGCCC 1 cut(s) 603
PspPPI RGGWCCY 1 cut(s) 722
PstNI CAGNNNCTG 2 cut(s) 1860, 1991
PsuI RGATCY 2 cut(s) 1315, 1594
PsyI GACNNNGTC 2 cut(s) 176, 1220
PteI GCGCGC 1 cut(s) 483
RigI GGCCGGCC 1 cut(s) 589
RruI TCGCGA 1 cut(s) 107
RsaI GTAC 7 cut(s) 332, 470, 503, 925, 1900, 1930, 2433
RsaNI GTAC 7 cut(s) 331, 469, 502, 924, 1899, 1929, 2432
RseI CAYNNNNRTG 5 cut(s) 578, 1082, 1361, 1941, 1985
SacII CCGCGG 1 cut(s) 1049
SalI GTCGAC 1 cut(s) 929
SaqAI TTAA 6 cut(s) 537, 681, 795, 807, 1416, 2244
Sau3AI GATC 4 cut(s) 772, 1315, 1536, 1594
ScaI AGTACT 1 cut(s) 332
SchI GAGTC 5 cut(s) 194, 230, 384, 2032, 2054
SduI GDGCHC 4 cut(s) 29, 607, 1111, 2155
SexAI ACCWGGT 1 cut(s) 1209
SfaNI GCATC 5 cut(s) 1756, 1958, 2038, 2170, 2257
SfcI CTRYAG 1 cut(s) 1732
Sfr274I CTCGAG 1 cut(s) 397
Sfr303I CCGCGG 1 cut(s) 1049
SgrAI CRCCGGYG 1 cut(s) 43
SgrBI CCGCGG 1 cut(s) 1049
SgsI GGCGCGCC 1 cut(s) 483
SinI GGWCC 5 cut(s) 97, 701, 722, 1971, 1977
SlaI CTCGAG 1 cut(s) 397
SmaI CCCGGG 1 cut(s) 715
SmiMI CAYNNNNRTG 5 cut(s) 578, 1082, 1361, 1941, 1985
SmlI CTYRAG 3 cut(s) 298, 397, 1415
SmoI CTYRAG 3 cut(s) 298, 397, 1415
SpeI ACTAGT 2 cut(s) 1249, 1544
SseBI AGGCCT 3 cut(s) 1232, 1730, 1847
SspI AATATT 1 cut(s) 868
SspMI CTAG 9 cut(s) 345, 351, 1092, 1184, 1250, 1545, 2256, 2288, 2420
StuI AGGCCT 3 cut(s) 1232, 1730, 1847
StyI CCWWGG 2 cut(s) 1467, 2255
TaaI ACNGT 9 cut(s) 223, 928, 1126, 1292, 1642, 1651, 1672, 1993, 2344
TaiI ACGT 4 cut(s) 180, 474, 1493, 1934
TaqI TCGA 9 cut(s) 168, 198, 398, 426, 477, 642, 930, 1539, 2251
TaqII GACCGA 1 cut(s) 785
TatI WGTACW 4 cut(s) 330, 501, 923, 1898
TfiI GAWTC 9 cut(s) 102, 213, 644, 1099, 1162, 1430, 1445, 2168, 2375
Tru1I TTAA 6 cut(s) 537, 681, 795, 807, 1416, 2244
Tru9I TTAA 6 cut(s) 537, 681, 795, 807, 1416, 2244
TscAI CASTG 6 cut(s) 1654, 1971, 1992, 2008, 2244, 2323
TseFI GTSAC 2 cut(s) 1283, 1987
TseI GCWGC 2 cut(s) 2096, 2267
Tsp45I GTSAC 2 cut(s) 1283, 1987
TspGWI ACGGA 3 cut(s) 107, 436, 693
TspMI CCCGGG 1 cut(s) 713
TspRI CASTG 6 cut(s) 1654, 1971, 1992, 2008, 2244, 2323
Tth111I GACNNNGTC 2 cut(s) 176, 1220
Vha464I CTTAAG 1 cut(s) 1415
VneI GTGCAC 1 cut(s) 2151
VpaK11BI GGWCC 5 cut(s) 97, 701, 722, 1971, 1977
XapI RAATTY 2 cut(s) 10, 134
XbaI TCTAGA 1 cut(s) 2419
XceI RCATGY 1 cut(s) 859
XhoI CTCGAG 1 cut(s) 397
XmaI CCCGGG 1 cut(s) 713
XmaJI CCTAGG 1 cut(s) 2255
XmiI GTMKAC 3 cut(s) 930, 1200, 1215
XmnI GAANNNNTTC 1 cut(s) 638
XspI CTAG 9 cut(s) 345, 351, 1092, 1184, 1250, 1545, 2256, 2288, 2420
ZraI GACGTC 1 cut(s) 178
ZrmI AGTACT 1 cut(s) 332
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.