MD02G1059500.v1.1

CRS2-associated factor 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0013996
Physical Location & Seq
Reverse (-)
4777245 .. 4780403
3159 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1059500.v1.1.491

Sequence Viewer

Length: 2334 bp
ATGGCGCTGAATTTGCCCACTTCCTGTCCGATTTTCGCGCCACTATTGAACCCGAGCCCCACCCACAACGCGGCTCAGAACCGCCTGCCAACGGAGGTCCGATTAGCGCGGTGGAACAACGCCAACGCTGAGAAATTCAACCAGCGCCGGAGGGCCCAGCAAGAAATCGAGGACGATATTCGCCGGGAGCGTCGATTCGAGTCCGCCACCAGAATTGCCACTCTTTCCGACTCTGCCACTGACACCACCACCGCCGCTGCCAACGCCACGACGAGTAGCGAAACATTTAAATCCATCGGCACGCCGTCGTATCCCTCGAGCCCTTCAATCCCAGGCAAGAAATCCAAGTACTCCAAAAACCCTAACCCTAACGATTCGCACCCGGCATTTCGCCGAATCATAAGACCCACGAAGGTCTCGAAAATTTTGAGGGAGAAAAAGCCGGCAGTCGACAGAAAAGCCGACATTTCCATCCGCGACGATGGGCTTTCTTACGTCATCGACGGAGCCCCTTTTGAATTCAAGTACAGCTACACCGAGACTCCTAAAATCAAGCCCATTAAGCTCCGGGAACCGCCGTTCTTACCATTTGGGCCCACCACGATGGACCGGCCATGGACGGGTCGGGCCCCGTTGCCAGCAAGCAAGAAGAAACTGAAGGAATTCGACTCTTTCCAGCTGCCTCCGCCGCACAAGAAGGGGGTTAAGCCGGTGCAATCTCCGGGTCCGTATTTGGCAGGGTCGGGTCCTAAGTATGTGAAGTCCAGGGAGGAGATTTTGGGGGAGCCGCTGACAGTAGAGGAAGTTAAGGAATTAGTTAAAGGGTGCATCAAAACCAAGAGGCAGTTGAATATGGGTAGAGATGGTTTGACCCACAACATGTTGGATAACATACATGCTCACTGGAAGCGCCGGAGAGTGTGCAAGATTAAATGCAAAGGTGTTTGTACAGTCGATATGGAGAATGTGTGCGAGCAATTAGAGGAAAGAACTGGAGGGAAGATCATTTACAGGAAAGGTGGAGTGATATACCTTTTCCGAGGCAGAAACTATAACTATAAAACTCGGCCTAAGTTTCCTCTCATGCTTTGGAGACCTATTACTCCTGTGTATCCTAGACTGGTTCAGCGGGCTCCCGAGGGTCTAACAGTAGAAGAAGCATCTGAGATGCGTAAGAAGGGGCGGAATCTGATACCAATATGCAAGCTAGGGAAAAATGGTGTATACTCTGAATTGGTAAAAAATGTCAGAGAGGCCTTTGAAGAGTGTGAGCTAGTGCGAATAAATTGTCAGGGGATGAACGGAAGTGACTACCGAAAAATAGGTGCTAAACTAAAGGATCTTGTCCCTTGTGTGTTAATGTCATTTGAACTTGAGCACATTCTTTTGTGGAGGGGACGGGAATGGAAGTCTTCTCTTCTAAATCCAGAGAATGATCTCAAGGAAGTCAACGGATCTGATGTTGATGATTCAACTTCAATTGCATCAACATCATGTACTTCTGAAGTTGTGGGTGCAGAAGGAAGTGAGGATCTCTCGCCCTCACTATATGTTGAGCCACGTGTCACAGTTGATAGGGTTGATAGGGCTTCTACTGTTGGAGGAACCTCTGAAACCAAACCCATTTCAGATGTTGAAGGCTATATAAACAATAAATCAGAAGCTAAAATGAATTCCGATAACAGCTCAACCATACCAGACAATATCCACTATGCTGATGATAAGTCAAAAACTACGCCCCATACATCTGAAATTGAGCCCATGTTGGCTAACGCAGGCTGTGCTGATGAAGCATCACCAACTGCAGTCATGGGGTCTGGGACTATGGCAGTGCCTGTTGAAAGTGCTGAAACAAAGTTGGATTCCATCACGGCAGGTCCTGGAAGTGACGAGAATCCTCAAGATGCTTCGGCAGGCTCACAAATTCTTAGTGTGCCAGCAAAAGTGAGTGCACCTTGTACAGAAAATGTGCTGTTACTCTTGAATGAAGCTATTGGTAGAGGGAGTGCACTTATTTTAGACGAGTCATCCCTGGATGCCGATATTATTTATCAACGGTCAGTTGCCTTAGCGCAGTCTGCTCCCCCTGGCCCGGTGTTTAAGCATCAACGACCCAAGAAAGTAGCAGTTTTAAAGAGAGTAAAGGTTGTGAAACAAGATGGCGGGGATTCAGAAGTGAAAGAGATAACAGTTTCTGTGAAGAGAGGAAGTGAGAAGATTCAGAAGAAGGGTAGTAAAGTTCAAAGGACAAGAGATTTTGGTGAAAATCTACTAGATAATGTTGTACCACAGGGAAGTTTGAGAGTTGATGAACTTGCTAAGCTATTAGCATGA

Protein Analysis

778

Amino Acids

85.77

Weight (kDa)

9.18

Isoelectric Point (pI)

47.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRS1_YhbY PF01985 264 - 347 4.5e-21 CRS1 / YhbY (CRM) domain
CRS1_YhbY PF01985 382 - 465 3.7e-17 CRS1 / YhbY (CRM) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013996)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G20020
fragaria_vesca FvH4_1g05780
malus_domestica MD02G1059500.v1.1 MD15G1193500.v1.1
prunus_persica Prupe.7G223600_v2.0.a1
pyrus_communis pycom02g04850 pycom15g17320
rosa_chinensis RchiOBHm_Chr2g0091431
rosa_laevigata RLG00000016223
rosa_multiflora Rmu_ssc0000333.1_g000008
rosa_roxburghii Rroxscaffold_2G00150090
rosa_rugosa Rorug02G0017800
rosa_samantha Rh2AG062700 Rh2BG062000 Rh2CG063900 Rh2DG062300
rosa_wichuraiana Rw2G005740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1865
AccI GTMKAC 2 cut(s) 450, 1224
AccII CGCG 4 cut(s) 38, 71, 109, 477
AclWI GGATC 3 cut(s) 1347, 1462, 1539
AcoI YGGCCR 1 cut(s) 611
AcsI RAATTY 7 cut(s) 10, 134, 423, 518, 662, 1672, 1923
AcuI CTGAAG 2 cut(s) 677, 1524
AcvI CACGTG 1 cut(s) 1562
AfaI GTAC 6 cut(s) 350, 527, 949, 1498, 1960, 2286
AfiI CCNNNNNNNGG 4 cut(s) 70, 91, 620, 2092
AflIII ACRYGT 2 cut(s) 879, 1561
AjnI CCWGG 5 cut(s) 331, 764, 1879, 2031, 2086
AloI GAACNNNNNNTCC 2 cut(s) 563, 595
AluBI AGCT 9 cut(s) 531, 565, 679, 1207, 1273, 1664, 1686, 1991, 2323
AluI AGCT 9 cut(s) 531, 565, 679, 1207, 1273, 1664, 1686, 1991, 2323
Alw21I GWGCWC 3 cut(s) 1380, 1954, 2011
Alw26I GTCTC 3 cut(s) 421, 533, 1087
Alw44I GTGCAC 2 cut(s) 1950, 2007
AlwI GGATC 3 cut(s) 1347, 1462, 1539
AlwNI CAGNNNCTG 3 cut(s) 1835, 1880, 2195
Ama87I CYCGRG 3 cut(s) 52, 316, 1136
AoxI GGCC 7 cut(s) 153, 593, 611, 627, 1067, 1254, 2089
ApaI GGGCCC 3 cut(s) 157, 597, 631
ApaLI GTGCAC 2 cut(s) 1950, 2007
ApeKI GCWGC 2 cut(s) 257, 679
ApoI RAATTY 7 cut(s) 10, 134, 423, 518, 662, 1672, 1923
ArsI GACNNNNNNTTYG 2 cut(s) 1273, 1305
Asp700I GAANNNNTTC 1 cut(s) 662
AspLEI GCGC 6 cut(s) 7, 40, 109, 147, 912, 2074
AsuC2I CCSGG 5 cut(s) 185, 383, 569, 723, 2093
AsuHPI GGTGA 2 cut(s) 1788, 2273
AvaI CYCGRG 3 cut(s) 52, 316, 1136
AvaII GGWCC 5 cut(s) 97, 607, 725, 746, 1877
BaeGI GKGCMC 5 cut(s) 157, 597, 631, 1954, 2011
BanII GRGCYC 8 cut(s) 59, 157, 323, 511, 597, 631, 1135, 1761
BarI GAAGNNNNNNTAC 2 cut(s) 992, 1024
BbrPI CACGTG 1 cut(s) 1562
BbsI GAAGAC 1 cut(s) 1404
Bbv12I GWGCWC 3 cut(s) 1380, 1954, 2011
BbvI GCAGC 2 cut(s) 244, 666
BccI CCATC 7 cut(s) 302, 476, 479, 598, 857, 1874, 2153
BceAI ACGGC 3 cut(s) 289, 562, 1887
BciT130I CCWGG 5 cut(s) 333, 766, 1881, 2033, 2088
BciVI GTATCC 2 cut(s) 321, 1122
BcnI CCSGG 5 cut(s) 185, 383, 569, 723, 2093
BcoDI GTCTC 3 cut(s) 421, 533, 1087
BfaI CTAG 4 cut(s) 1116, 1208, 1274, 2273
BfmI CTRYAG 1 cut(s) 1803
BfoI RGCGCY 3 cut(s) 8, 148, 913
BfuAI ACCTGC 1 cut(s) 1865
BfuI GTATCC 2 cut(s) 321, 1122
BisI GCNGC 6 cut(s) 72, 255, 258, 680, 689, 788
BlpI GCTNAGC 1 cut(s) 2319
BlsI GCNGC 6 cut(s) 73, 256, 259, 681, 690, 789
BmcAI AGTACT 1 cut(s) 350
Bme18I GGWCC 5 cut(s) 97, 607, 725, 746, 1877
BmeT110I CYCGRG 3 cut(s) 52, 316, 1136
BmsI GCATC 8 cut(s) 837, 1158, 1169, 1493, 1802, 1894, 2026, 2113
BpiI GAAGAC 1 cut(s) 1404
BplI GAGNNNNNCTC 2 cut(s) 1520, 1552
BpmI CTGGAG 1 cut(s) 1014
Bpu10I CCTNAGC 1 cut(s) 2068
Bpu1102I GCTNAGC 1 cut(s) 2319
BpuEI CTTGAG 3 cut(s) 1394, 1424, 1884
BpuMI CCSGG 5 cut(s) 185, 383, 569, 723, 2093
BsaAI YACGTR 1 cut(s) 1562
BsaI GGTCTC 2 cut(s) 421, 1087
BsaJI CCNNGG 7 cut(s) 331, 614, 765, 1039, 1137, 2031, 2086
BsaXI ACNNNNNCTCC 4 cut(s) 526, 556, 953, 983
Bsc4I CCNNNNNNNGG 4 cut(s) 70, 91, 620, 2092
Bse118I RCCGGY 3 cut(s) 442, 609, 709
Bse1I ACTGG 3 cut(s) 908, 997, 1125
BseBI CCWGG 5 cut(s) 333, 766, 1881, 2033, 2088
BseDI CCNNGG 7 cut(s) 331, 614, 765, 1039, 1137, 2031, 2086
BseGI GGATG 4 cut(s) 471, 1302, 2027, 2041
BseLI CCNNNNNNNGG 4 cut(s) 70, 91, 620, 2092
BseMII CTCAG 3 cut(s) 89, 120, 1155
BseNI ACTGG 3 cut(s) 908, 997, 1125
BseRI GAGGAG 1 cut(s) 785
BseSI GKGCMC 5 cut(s) 157, 597, 631, 1954, 2011
BseXI GCAGC 2 cut(s) 244, 666
BseYI CCCAGC 1 cut(s) 156
BsgI GTGCAG 1 cut(s) 1536
Bsh1236I CGCG 4 cut(s) 38, 71, 109, 477
BshFI GGCC 7 cut(s) 155, 595, 613, 629, 1069, 1256, 2091
BsiHKAI GWGCWC 3 cut(s) 1380, 1954, 2011
BsiHKCI CYCGRG 3 cut(s) 52, 316, 1136
BslFI GGGAC 3 cut(s) 1331, 1410, 1834
BslI CCNNNNNNNGG 4 cut(s) 70, 91, 620, 2092
BsmAI GTCTC 3 cut(s) 421, 533, 1087
BsmFI GGGAC 3 cut(s) 1331, 1410, 1834
BsnI GGCC 7 cut(s) 155, 595, 613, 629, 1069, 1256, 2091
Bso31I GGTCTC 2 cut(s) 421, 1087
BsoBI CYCGRG 3 cut(s) 52, 316, 1136
Bsp120I GGGCCC 3 cut(s) 153, 593, 627
Bsp1407I TGTACA 2 cut(s) 947, 1958
Bsp143I GATC 5 cut(s) 1002, 1339, 1435, 1454, 1531
Bsp1720I GCTNAGC 1 cut(s) 2319
Bsp19I CCATGG 1 cut(s) 614
BspANI GGCC 7 cut(s) 155, 595, 613, 629, 1069, 1256, 2091
BspCNI CTCAG 3 cut(s) 88, 121, 1156
BspFNI CGCG 4 cut(s) 38, 71, 109, 477
BspMAI CTGCAG 1 cut(s) 1807
BspMI ACCTGC 1 cut(s) 1865
BspPI GGATC 3 cut(s) 1347, 1462, 1539
BspTNI GGTCTC 2 cut(s) 421, 1087
BsrFI RCCGGY 3 cut(s) 442, 609, 709
BsrGI TGTACA 2 cut(s) 947, 1958
BsrI ACTGG 3 cut(s) 908, 997, 1125
BssAI RCCGGY 3 cut(s) 442, 609, 709
BssECI CCNNGG 7 cut(s) 331, 614, 765, 1039, 1137, 2031, 2086
BssMI GATC 5 cut(s) 1002, 1339, 1435, 1454, 1531
BssNAI GTATAC 1 cut(s) 1225
BssT1I CCWWGG 1 cut(s) 614
Bst1107I GTATAC 1 cut(s) 1225
Bst2UI CCWGG 5 cut(s) 333, 766, 1881, 2033, 2088
Bst4CI ACNGT 7 cut(s) 796, 952, 1150, 1570, 1597, 2058, 2191
Bst6I CTCTTC 3 cut(s) 1257, 1422, 2195
BstAPI GCANNNNNTGC 1 cut(s) 1781
BstAUI TGTACA 2 cut(s) 947, 1958
BstBAI YACGTR 1 cut(s) 1562
BstDEI CTNAG 8 cut(s) 75, 129, 750, 1071, 1164, 1928, 2068, 2319
BstDSI CCRYGG 1 cut(s) 614
BstF5I GGATG 4 cut(s) 471, 1302, 2027, 2041
BstFNI CGCG 4 cut(s) 38, 71, 109, 477
BstH2I RGCGCY 3 cut(s) 8, 148, 913
BstHHI GCGC 6 cut(s) 7, 40, 109, 147, 912, 2074
BstKTI GATC 5 cut(s) 1005, 1342, 1438, 1457, 1534
BstMAI GTCTC 3 cut(s) 421, 533, 1087
BstMBI GATC 5 cut(s) 1002, 1339, 1435, 1454, 1531
BstMWI GCNNNNNNNGC 8 cut(s) 13, 263, 562, 685, 688, 1781, 1790, 2078
BstNI CCWGG 5 cut(s) 333, 766, 1881, 2033, 2088
BstNSI RCATGY 2 cut(s) 883, 899
BstSFI CTRYAG 1 cut(s) 1803
BstSLI GKGCMC 5 cut(s) 157, 597, 631, 1954, 2011
BstUI CGCG 4 cut(s) 38, 71, 109, 477
BstV1I GCAGC 2 cut(s) 244, 666
BstV2I GAAGAC 1 cut(s) 1404
BstX2I RGATCY 3 cut(s) 1339, 1454, 1531
BstXI CCANNNNNNTGG 1 cut(s) 604
BstYI RGATCY 3 cut(s) 1339, 1454, 1531
BstZ17I GTATAC 1 cut(s) 1225
BsuI GTATCC 2 cut(s) 321, 1122
BsuRI GGCC 7 cut(s) 155, 595, 613, 629, 1069, 1256, 2091
BtgI CCRYGG 1 cut(s) 614
BtsCI GGATG 4 cut(s) 471, 1302, 2027, 2041
BtsI GCAGTG 1 cut(s) 1836
BtsIMutI CAGTG 3 cut(s) 237, 901, 1836
BveI ACCTGC 1 cut(s) 1865
CaiI CAGNNNCTG 3 cut(s) 1835, 1880, 2195
CfoI GCGC 6 cut(s) 7, 40, 109, 147, 912, 2074
Cfr10I RCCGGY 3 cut(s) 442, 609, 709
CseI GACGC 1 cut(s) 179
Csp6I GTAC 6 cut(s) 349, 526, 948, 1497, 1959, 2285
CspCI CAANNNNNGTGG 2 cut(s) 196, 231
CviAII CATG 8 cut(s) 615, 880, 896, 1084, 1494, 1762, 1810, 2331
CviQI GTAC 6 cut(s) 349, 526, 948, 1497, 1959, 2285
DdeI CTNAG 8 cut(s) 75, 129, 750, 1071, 1164, 1928, 2068, 2319
DpnI GATC 5 cut(s) 1004, 1341, 1437, 1456, 1533
DpnII GATC 5 cut(s) 1002, 1339, 1435, 1454, 1531
DraI TTTAAA 2 cut(s) 289, 2133
EaeI YGGCCR 1 cut(s) 611
Eam1104I CTCTTC 3 cut(s) 1257, 1422, 2195
EarI CTCTTC 3 cut(s) 1257, 1422, 2195
EciI GGCGGA 3 cut(s) 193, 675, 1198
Eco130I CCWWGG 1 cut(s) 614
Eco147I AGGCCT 1 cut(s) 1256
Eco24I GRGCYC 8 cut(s) 59, 157, 323, 511, 597, 631, 1135, 1761
Eco31I GGTCTC 2 cut(s) 421, 1087
Eco47I GGWCC 5 cut(s) 97, 607, 725, 746, 1877
Eco57I CTGAAG 2 cut(s) 677, 1524
Eco72I CACGTG 1 cut(s) 1562
Eco88I CYCGRG 3 cut(s) 52, 316, 1136
EcoO109I RGGNCCY 4 cut(s) 153, 628, 746, 1877
EcoRI GAATTC 3 cut(s) 518, 662, 1672
EcoRII CCWGG 5 cut(s) 331, 764, 1879, 2031, 2086
EcoT14I CCWWGG 1 cut(s) 614
EcoT38I GRGCYC 8 cut(s) 59, 157, 323, 511, 597, 631, 1135, 1761
ErhI CCWWGG 1 cut(s) 614
FaeI CATG 8 cut(s) 618, 883, 899, 1087, 1497, 1765, 1813, 2334
FaqI GGGAC 3 cut(s) 1331, 1410, 1834
FatI CATG 8 cut(s) 614, 879, 895, 1083, 1493, 1761, 1809, 2330
FauI CCCGC 2 cut(s) 1122, 2156
FblI GTMKAC 2 cut(s) 450, 1224
Fnu4HI GCNGC 6 cut(s) 72, 255, 258, 680, 689, 788
FokI GGATG 4 cut(s) 458, 1309, 2014, 2048
FriOI GRGCYC 8 cut(s) 59, 157, 323, 511, 597, 631, 1135, 1761
Fsp4HI GCNGC 6 cut(s) 72, 255, 258, 680, 689, 788
FspBI CTAG 4 cut(s) 1116, 1208, 1274, 2273
GlaI GCGC 6 cut(s) 6, 39, 108, 146, 911, 2073
GluI GCNGC 6 cut(s) 72, 255, 258, 680, 689, 788
GsaI CCCAGC 1 cut(s) 160
GsuI CTGGAG 1 cut(s) 1014
HaeII RGCGCY 3 cut(s) 8, 148, 913
HaeIII GGCC 7 cut(s) 155, 595, 613, 629, 1069, 1256, 2091
HgaI GACGC 1 cut(s) 179
HhaI GCGC 6 cut(s) 7, 40, 109, 147, 912, 2074
Hin1II CATG 8 cut(s) 618, 883, 899, 1087, 1497, 1765, 1813, 2334
Hin6I GCGC 6 cut(s) 5, 38, 107, 145, 910, 2072
HinP1I GCGC 6 cut(s) 5, 38, 107, 145, 910, 2072
HincII GTYRAC 2 cut(s) 451, 1450
HindII GTYRAC 2 cut(s) 451, 1450
HphI GGTGA 2 cut(s) 1788, 2273
Hpy166II GTNNAC 5 cut(s) 451, 1225, 1450, 1952, 2009
Hpy188III TCNNGA 5 cut(s) 418, 1136, 1427, 1901, 1981
Hpy8I GTNNAC 5 cut(s) 451, 1225, 1450, 1952, 2009
Hpy99I CGWCG 5 cut(s) 195, 274, 310, 482, 506
HpyAV CCTTC 8 cut(s) 333, 406, 652, 691, 1171, 1514, 1631, 2221
HpyCH4III ACNGT 7 cut(s) 796, 952, 1150, 1570, 1597, 2058, 2191
HpyCH4IV ACGT 2 cut(s) 495, 1561
HpyF10VI GCNNNNNNNGC 8 cut(s) 13, 263, 562, 685, 688, 1781, 1790, 2078
HpyF3I CTNAG 8 cut(s) 75, 129, 750, 1071, 1164, 1928, 2068, 2319
HpySE526I ACGT 2 cut(s) 495, 1561
Hsp92II CATG 8 cut(s) 618, 883, 899, 1087, 1497, 1765, 1813, 2334
HspAI GCGC 6 cut(s) 5, 38, 107, 145, 910, 2072
KroI GCCGGC 1 cut(s) 442
KroNI GCCGGC 1 cut(s) 444
Kzo9I GATC 5 cut(s) 1002, 1339, 1435, 1454, 1531
LmnI GCTCC 6 cut(s) 187, 506, 570, 784, 1138, 2086
Lsp1109I GCAGC 2 cut(s) 244, 666
LweI GCATC 8 cut(s) 837, 1158, 1169, 1493, 1802, 1894, 2026, 2113
MaeI CTAG 4 cut(s) 1116, 1208, 1274, 2273
MaeII ACGT 2 cut(s) 495, 1561
MaeIII GTNAC 4 cut(s) 1307, 1564, 1886, 1974
MalI GATC 5 cut(s) 1004, 1341, 1437, 1456, 1533
MboI GATC 5 cut(s) 1002, 1339, 1435, 1454, 1531
MboII GAAGA 9 cut(s) 661, 1012, 1166, 1274, 1404, 1409, 2212, 2227, 2236
MfeI CAATTG 1 cut(s) 1479
MflI RGATCY 3 cut(s) 1339, 1454, 1531
MlyI GAGTC 5 cut(s) 209, 224, 535, 662, 2033
MmeI TCCRAC 4 cut(s) 252, 864, 1579, 1839
MroNI GCCGGC 1 cut(s) 442
MroXI GAANNNNTTC 1 cut(s) 662
MseI TTAA 9 cut(s) 288, 561, 705, 807, 819, 930, 1358, 2100, 2132
MslI CAYNNNNRTG 1 cut(s) 602
MspA1I CMGCKG 4 cut(s) 257, 679, 790, 1129
MunI CAATTG 1 cut(s) 1479
MvaI CCWGG 5 cut(s) 333, 766, 1881, 2033, 2088
MvnI CGCG 4 cut(s) 38, 71, 109, 477
MwoI GCNNNNNNNGC 8 cut(s) 13, 263, 562, 685, 688, 1781, 1790, 2078
NaeI GCCGGC 1 cut(s) 444
NciI CCSGG 5 cut(s) 185, 383, 569, 723, 2093
NcoI CCATGG 1 cut(s) 614
NdeII GATC 5 cut(s) 1002, 1339, 1435, 1454, 1531
NgoMIV GCCGGC 1 cut(s) 442
NlaIII CATG 8 cut(s) 618, 883, 899, 1087, 1497, 1765, 1813, 2334
NmeAIII GCCGAG 1 cut(s) 1045
NmuCI GTSAC 3 cut(s) 1307, 1564, 1886
NspI RCATGY 2 cut(s) 883, 899
PaeR7I CTCGAG 1 cut(s) 316
PceI AGGCCT 1 cut(s) 1256
PciI ACATGT 1 cut(s) 879
PcsI WCGNNNNNNNCGW 3 cut(s) 187, 314, 416
PdiI GCCGGC 1 cut(s) 444
PdmI GAANNNNTTC 1 cut(s) 662
PfeI GAWTC 9 cut(s) 195, 374, 396, 1186, 1469, 1862, 1894, 2168, 2218
PfoI TCCNGGA 2 cut(s) 567, 1879
PkrI GCNGC 6 cut(s) 73, 256, 259, 681, 690, 789
PleI GAGTC 5 cut(s) 208, 224, 535, 662, 2032
PmaCI CACGTG 1 cut(s) 1562
PmlI CACGTG 1 cut(s) 1562
PpsI GAGTC 5 cut(s) 208, 224, 535, 662, 2032
Ppu21I YACGTR 1 cut(s) 1562
PpuMI RGGWCCY 2 cut(s) 746, 1877
PscI ACATGT 1 cut(s) 879
Psp5II RGGWCCY 2 cut(s) 746, 1877
Psp6I CCWGG 5 cut(s) 331, 764, 1879, 2031, 2086
PspCI CACGTG 1 cut(s) 1562
PspFI CCCAGC 1 cut(s) 156
PspGI CCWGG 5 cut(s) 331, 764, 1879, 2031, 2086
PspOMI GGGCCC 3 cut(s) 153, 593, 627
PspPPI RGGWCCY 2 cut(s) 746, 1877
PspXI VCTCGAGB 1 cut(s) 316
PstI CTGCAG 1 cut(s) 1807
PstNI CAGNNNCTG 3 cut(s) 1835, 1880, 2195
PsuI RGATCY 3 cut(s) 1339, 1454, 1531
PvuII CAGCTG 1 cut(s) 679
RsaI GTAC 6 cut(s) 350, 527, 949, 1498, 1960, 2286
RsaNI GTAC 6 cut(s) 349, 526, 948, 1497, 1959, 2285
RseI CAYNNNNRTG 1 cut(s) 602
SalI GTCGAC 1 cut(s) 449
SaqAI TTAA 9 cut(s) 288, 561, 705, 807, 819, 930, 1358, 2100, 2132
SatI GCNGC 6 cut(s) 72, 255, 258, 680, 689, 788
Sau3AI GATC 5 cut(s) 1002, 1339, 1435, 1454, 1531
ScaI AGTACT 1 cut(s) 350
SchI GAGTC 5 cut(s) 209, 224, 535, 662, 2033
SfaNI GCATC 8 cut(s) 837, 1158, 1169, 1493, 1802, 1894, 2026, 2113
SfcI CTRYAG 1 cut(s) 1803
Sfr274I CTCGAG 1 cut(s) 316
SinI GGWCC 5 cut(s) 97, 607, 725, 746, 1877
SlaI CTCGAG 1 cut(s) 316
SmiI ATTTAAAT 1 cut(s) 289
SmiMI CAYNNNNRTG 1 cut(s) 602
SmlI CTYRAG 4 cut(s) 316, 1373, 1439, 1899
SmoI CTYRAG 4 cut(s) 316, 1373, 1439, 1899
SseBI AGGCCT 1 cut(s) 1256
SspMI CTAG 4 cut(s) 1116, 1208, 1274, 2273
StuI AGGCCT 1 cut(s) 1256
StyI CCWWGG 1 cut(s) 614
SwaI ATTTAAAT 1 cut(s) 289
TaaI ACNGT 7 cut(s) 796, 952, 1150, 1570, 1597, 2058, 2191
TaiI ACGT 2 cut(s) 498, 1564
TaqI TCGA 9 cut(s) 168, 193, 198, 317, 419, 450, 501, 666, 954
TatI WGTACW 5 cut(s) 348, 525, 947, 1496, 1958
TauI GCSGC 4 cut(s) 74, 257, 691, 790
TfiI GAWTC 9 cut(s) 195, 374, 396, 1186, 1469, 1862, 1894, 2168, 2218
Tru1I TTAA 9 cut(s) 288, 561, 705, 807, 819, 930, 1358, 2100, 2132
Tru9I TTAA 9 cut(s) 288, 561, 705, 807, 819, 930, 1358, 2100, 2132
TscAI CASTG 3 cut(s) 244, 908, 1836
TseFI GTSAC 3 cut(s) 1307, 1564, 1886
TseI GCWGC 2 cut(s) 257, 679
Tsp45I GTSAC 3 cut(s) 1307, 1564, 1886
TspDTI ATGAA 5 cut(s) 1313, 1685, 1803, 2001, 2325
TspGWI ACGGA 5 cut(s) 107, 519, 717, 1317, 1467
TspRI CASTG 3 cut(s) 244, 908, 1836
VneI GTGCAC 2 cut(s) 1950, 2007
VpaK11BI GGWCC 5 cut(s) 97, 607, 725, 746, 1877
XapI RAATTY 7 cut(s) 10, 134, 423, 518, 662, 1672, 1923
XceI RCATGY 2 cut(s) 883, 899
XhoI CTCGAG 1 cut(s) 316
XmiI GTMKAC 2 cut(s) 450, 1224
XmnI GAANNNNTTC 1 cut(s) 662
XspI CTAG 4 cut(s) 1116, 1208, 1274, 2273
ZrmI AGTACT 1 cut(s) 350
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.