Rorug02G0017800

CRS2-associated factor 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0013996
Physical Location & Seq
Reverse (-)
1471672 .. 1473767
2096 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0017800.1

Sequence Viewer

Length: 858 bp
ATGAAGCTTTTCACTATGCTTATGGCTTTGCTATTTCAGTCCTTTTTGTTGCAAGCTCAGGTGAGCTTACCACCACCTCCCATTCCTAGCCCTGATCCTTCCCTGGGGTTAGTACCACTAGGTGTTCCTTATTATTATAGTTATATCCCACCTCCTCCTCCCGTTGTTCTTCCACCACCACCACAACCGCCACCATCAGGACCACCTCCCATTCCCAGCCCTGATCCTTCCCAGGGGTTAGTACCACCAGGTGTTCCTTATTATTATAGCTATATCCCACCTCCTCCTCCCGTTGTTCTTCCCCCACCGCCACCACCTCCCCTTGTTCTTCCCCCAGCGCCACAACCGCCACCATCAGGTCCTAATCCGGCTGATAGAGCTCTTTGTACTGTGCAATGTAGTGCTGATTGTGCCTCGATCTTTGGTCCATCGATACAACCCAATAAATTTCGGCGCACCCCCAGAACTCCCCCCAGCAATCCCACAACTCCTAGCACACCCACAACTCCTGGCACTCCCACAACTCCCAGTACTCCCTCAGCTCCGGGCACGCCTGCAACTCCGGGCACTCCTACAACTCCTGGTACTCCGGCAGCTCCGGGCACACCTGCAACTCCGGGCACTCCAACTCCTGGTACTCCCGCAGCTCCGAGTACGCCTGCAACTCCGGGCACTCCAAACGCTCCTGGTACTCCCACAGCTCCGAGCACGCCTGCAACTCCGGGCACTCCAAACATTCCCAGTCCTCCCGGTTGGAAACGATTCGAAATACCAAGGGATATTCCTAAGGCAGCTTGTATACCTGTGTGTATTGGAATATGCATGAATATAAGCCCCGCACCCCCACGCATGTTCTAG

Protein Analysis

285

Amino Acids

28.98

Weight (kDa)

8.98

Isoelectric Point (pI)

78.4

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013996)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G20020
fragaria_vesca FvH4_1g05780
malus_domestica MD02G1059500.v1.1 MD15G1193500.v1.1
prunus_persica Prupe.7G223600_v2.0.a1
pyrus_communis pycom02g04850 pycom15g17320
rosa_chinensis RchiOBHm_Chr2g0091431
rosa_laevigata RLG00000016223
rosa_multiflora Rmu_ssc0000333.1_g000008
rosa_roxburghii Rroxscaffold_2G00150090
rosa_rugosa Rorug02G0017800
rosa_samantha Rh2AG062700 Rh2BG062000 Rh2CG063900 Rh2DG062300
rosa_wichuraiana Rw2G005740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 616
Acc36I ACCTGC 1 cut(s) 616
AccB7I CCANNNNNTGG 1 cut(s) 632
AccI GTMKAC 1 cut(s) 799
AciI CCGC 5 cut(s) 188, 308, 347, 642, 837
AclWI GGATC 2 cut(s) 89, 218
AcsI RAATTY 1 cut(s) 446
AdeI CACNNNGTG 2 cut(s) 122, 251
AfaI GTAC 8 cut(s) 114, 243, 388, 532, 586, 637, 655, 691
AfiI CCNNNNNNNGG 5 cut(s) 104, 197, 233, 356, 632
AjnI CCWGG 7 cut(s) 102, 231, 247, 508, 580, 631, 685
Alw21I GWGCWC 2 cut(s) 382, 710
AlwI GGATC 2 cut(s) 89, 218
ApeKI GCWGC 3 cut(s) 593, 644, 791
ApoI RAATTY 1 cut(s) 446
Asp700I GAANNNNTTC 2 cut(s) 8, 761
AspLEI GCGC 2 cut(s) 340, 456
AspS9I GGNCC 3 cut(s) 200, 359, 425
AsuC2I CCSGG 7 cut(s) 546, 564, 600, 618, 669, 723, 750
AsuHPI GGTGA 1 cut(s) 73
AsuII TTCGAA 1 cut(s) 765
AvaII GGWCC 3 cut(s) 200, 359, 425
AxyI CCTNAGG 1 cut(s) 786
BaeGI GKGCMC 6 cut(s) 551, 569, 605, 623, 674, 728
BanII GRGCYC 1 cut(s) 382
Bbv12I GWGCWC 2 cut(s) 382, 710
BbvCI CCTCAGC 1 cut(s) 538
BbvI GCAGC 3 cut(s) 605, 656, 803
BccI CCATC 3 cut(s) 202, 361, 436
BciT130I CCWGG 7 cut(s) 104, 233, 249, 510, 582, 633, 687
BcnI CCSGG 7 cut(s) 546, 564, 600, 618, 669, 723, 750
BfaI CTAG 4 cut(s) 87, 119, 492, 856
BfoI RGCGCY 1 cut(s) 341
BfuAI ACCTGC 1 cut(s) 616
BisI GCNGC 3 cut(s) 594, 645, 792
BlsI GCNGC 3 cut(s) 595, 646, 793
BmcAI AGTACT 1 cut(s) 532
Bme18I GGWCC 3 cut(s) 200, 359, 425
BmgT120I GGNCC 3 cut(s) 200, 359, 425
BmrI ACTGGG 2 cut(s) 522, 735
BmuI ACTGGG 2 cut(s) 522, 735
Bpu10I CCTNAGC 2 cut(s) 57, 538
Bpu14I TTCGAA 1 cut(s) 765
BpuMI CCSGG 7 cut(s) 546, 564, 600, 618, 669, 723, 750
Bsa29I ATCGAT 1 cut(s) 431
BsaJI CCNNGG 5 cut(s) 102, 103, 231, 232, 773
BsaXI ACNNNNNCTCC 2 cut(s) 613, 643
Bsc4I CCNNNNNNNGG 5 cut(s) 104, 197, 233, 356, 632
Bse1I ACTGG 2 cut(s) 528, 741
Bse21I CCTNAGG 1 cut(s) 786
Bse3DI GCAATG 1 cut(s) 401
BseBI CCWGG 7 cut(s) 104, 233, 249, 510, 582, 633, 687
BseCI ATCGAT 1 cut(s) 431
BseDI CCNNGG 5 cut(s) 102, 103, 231, 232, 773
BseLI CCNNNNNNNGG 5 cut(s) 104, 197, 233, 356, 632
BseMI GCAATG 1 cut(s) 401
BseMII CTCAG 2 cut(s) 71, 552
BseNI ACTGG 2 cut(s) 528, 741
BseRI GAGGAG 4 cut(s) 144, 147, 273, 276
BseSI GKGCMC 6 cut(s) 551, 569, 605, 623, 674, 728
BseXI GCAGC 3 cut(s) 605, 656, 803
BseYI CCCAGC 3 cut(s) 215, 334, 473
BshVI ATCGAT 1 cut(s) 431
BsiHKAI GWGCWC 2 cut(s) 382, 710
BsiSI CCGG 9 cut(s) 368, 545, 563, 590, 599, 617, 668, 722, 750
BslI CCNNNNNNNGG 5 cut(s) 104, 197, 233, 356, 632
Bsp119I TTCGAA 1 cut(s) 765
Bsp1286I GDGCHC 8 cut(s) 382, 551, 569, 605, 623, 674, 710, 728
Bsp143I GATC 3 cut(s) 94, 223, 417
BspACI CCGC 5 cut(s) 188, 308, 347, 642, 837
BspCNI CTCAG 2 cut(s) 70, 551
BspDI ATCGAT 1 cut(s) 431
BspMI ACCTGC 1 cut(s) 616
BspPI GGATC 2 cut(s) 89, 218
BspT104I TTCGAA 1 cut(s) 765
BsrDI GCAATG 1 cut(s) 401
BsrI ACTGG 2 cut(s) 528, 741
BssECI CCNNGG 5 cut(s) 102, 103, 231, 232, 773
BssMI GATC 3 cut(s) 94, 223, 417
BssNAI GTATAC 1 cut(s) 800
BssT1I CCWWGG 1 cut(s) 773
Bst1107I GTATAC 1 cut(s) 800
Bst2UI CCWGG 7 cut(s) 104, 233, 249, 510, 582, 633, 687
Bst4CI ACNGT 1 cut(s) 391
BstBI TTCGAA 1 cut(s) 765
BstC8I GCNNGC 6 cut(s) 54, 551, 555, 660, 710, 714
BstDEI CTNAG 3 cut(s) 57, 538, 786
BstH2I RGCGCY 1 cut(s) 341
BstHHI GCGC 2 cut(s) 340, 456
BstKTI GATC 3 cut(s) 97, 226, 420
BstMBI GATC 3 cut(s) 94, 223, 417
BstMWI GCNNNNNNNGC 3 cut(s) 346, 377, 410
BstNI CCWGG 7 cut(s) 104, 233, 249, 510, 582, 633, 687
BstNSI RCATGY 1 cut(s) 853
BstSLI GKGCMC 6 cut(s) 551, 569, 605, 623, 674, 728
BstV1I GCAGC 3 cut(s) 605, 656, 803
BstZ17I GTATAC 1 cut(s) 800
Bsu15I ATCGAT 1 cut(s) 431
Bsu36I CCTNAGG 1 cut(s) 786
BsuTUI ATCGAT 1 cut(s) 431
BveI ACCTGC 1 cut(s) 616
Cac8I GCNNGC 6 cut(s) 54, 551, 555, 660, 710, 714
CfoI GCGC 2 cut(s) 340, 456
Cfr13I GGNCC 3 cut(s) 200, 359, 425
ClaI ATCGAT 1 cut(s) 431
CsiI ACCWGGT 1 cut(s) 247
Csp6I GTAC 8 cut(s) 113, 242, 387, 531, 585, 636, 654, 690
CviAII CATG 2 cut(s) 823, 850
CviQI GTAC 8 cut(s) 113, 242, 387, 531, 585, 636, 654, 690
DdeI CTNAG 3 cut(s) 57, 538, 786
DpnI GATC 3 cut(s) 96, 225, 419
DpnII GATC 3 cut(s) 94, 223, 417
DraIII CACNNNGTG 2 cut(s) 122, 251
Ecl136II GAGCTC 1 cut(s) 380
Eco130I CCWWGG 1 cut(s) 773
Eco24I GRGCYC 1 cut(s) 382
Eco47I GGWCC 3 cut(s) 200, 359, 425
Eco53kI GAGCTC 1 cut(s) 380
Eco81I CCTNAGG 1 cut(s) 786
EcoICRI GAGCTC 1 cut(s) 380
EcoO109I RGGNCCY 1 cut(s) 359
EcoRII CCWGG 7 cut(s) 102, 231, 247, 508, 580, 631, 685
EcoT14I CCWWGG 1 cut(s) 773
EcoT22I ATGCAT 1 cut(s) 824
EcoT38I GRGCYC 1 cut(s) 382
ErhI CCWWGG 1 cut(s) 773
FaeI CATG 2 cut(s) 826, 853
FatI CATG 2 cut(s) 822, 849
FauI CCCGC 2 cut(s) 649, 844
FblI GTMKAC 1 cut(s) 799
Fnu4HI GCNGC 3 cut(s) 594, 645, 792
FriOI GRGCYC 1 cut(s) 382
Fsp4HI GCNGC 3 cut(s) 594, 645, 792
FspBI CTAG 4 cut(s) 87, 119, 492, 856
GlaI GCGC 2 cut(s) 339, 455
GluI GCNGC 3 cut(s) 594, 645, 792
GsaI CCCAGC 3 cut(s) 219, 338, 477
HaeII RGCGCY 1 cut(s) 341
HapII CCGG 9 cut(s) 368, 545, 563, 590, 599, 617, 668, 722, 750
HhaI GCGC 2 cut(s) 340, 456
Hin1II CATG 2 cut(s) 826, 853
Hin6I GCGC 2 cut(s) 338, 454
HinP1I GCGC 2 cut(s) 338, 454
HindIII AAGCTT 1 cut(s) 5
HinfI GANTC 1 cut(s) 762
HpaII CCGG 9 cut(s) 368, 545, 563, 590, 599, 617, 668, 722, 750
HphI GGTGA 1 cut(s) 73
Hpy166II GTNNAC 1 cut(s) 800
Hpy188I TCNGA 2 cut(s) 651, 705
Hpy188III TCNNGA 1 cut(s) 198
Hpy8I GTNNAC 1 cut(s) 800
HpyAV CCTTC 2 cut(s) 108, 237
HpyCH4III ACNGT 1 cut(s) 391
HpyCH4V TGCA 7 cut(s) 52, 394, 557, 611, 662, 716, 822
HpyF10VI GCNNNNNNNGC 3 cut(s) 346, 377, 410
HpyF3I CTNAG 3 cut(s) 57, 538, 786
Hsp92II CATG 2 cut(s) 826, 853
HspAI GCGC 2 cut(s) 338, 454
Kzo9I GATC 3 cut(s) 94, 223, 417
LmnI GCTCC 5 cut(s) 547, 601, 652, 688, 706
Lsp1109I GCAGC 3 cut(s) 605, 656, 803
MabI ACCWGGT 1 cut(s) 247
MaeI CTAG 4 cut(s) 87, 119, 492, 856
MalI GATC 3 cut(s) 96, 225, 419
MboI GATC 3 cut(s) 94, 223, 417
MboII GAAGA 3 cut(s) 161, 290, 320
MhlI GDGCHC 8 cut(s) 382, 551, 569, 605, 623, 674, 710, 728
MluCI AATT 1 cut(s) 446
MmeI TCCRAC 2 cut(s) 650, 734
Mph1103I ATGCAT 1 cut(s) 824
MroXI GAANNNNTTC 2 cut(s) 8, 761
MspI CCGG 9 cut(s) 368, 545, 563, 590, 599, 617, 668, 722, 750
MvaI CCWGG 7 cut(s) 104, 233, 249, 510, 582, 633, 687
MwoI GCNNNNNNNGC 3 cut(s) 346, 377, 410
NciI CCSGG 7 cut(s) 546, 564, 600, 618, 669, 723, 750
NdeII GATC 3 cut(s) 94, 223, 417
NlaIII CATG 2 cut(s) 826, 853
NsiI ATGCAT 1 cut(s) 824
NspI RCATGY 1 cut(s) 853
NspV TTCGAA 1 cut(s) 765
PaqCI CACCTGC 1 cut(s) 616
PasI CCCWGGG 2 cut(s) 103, 232
PdmI GAANNNNTTC 2 cut(s) 8, 761
PfeI GAWTC 1 cut(s) 762
PflMI CCANNNNNTGG 1 cut(s) 632
PkrI GCNGC 3 cut(s) 595, 646, 793
PpuMI RGGWCCY 1 cut(s) 359
Psp124BI GAGCTC 1 cut(s) 382
Psp5II RGGWCCY 1 cut(s) 359
Psp6I CCWGG 7 cut(s) 102, 231, 247, 508, 580, 631, 685
PspFI CCCAGC 3 cut(s) 215, 334, 473
PspGI CCWGG 7 cut(s) 102, 231, 247, 508, 580, 631, 685
PspPI GGNCC 3 cut(s) 200, 359, 425
PspPPI RGGWCCY 1 cut(s) 359
RsaI GTAC 8 cut(s) 114, 243, 388, 532, 586, 637, 655, 691
RsaNI GTAC 8 cut(s) 113, 242, 387, 531, 585, 636, 654, 690
SacI GAGCTC 1 cut(s) 382
SatI GCNGC 3 cut(s) 594, 645, 792
Sau3AI GATC 3 cut(s) 94, 223, 417
Sau96I GGNCC 3 cut(s) 200, 359, 425
ScaI AGTACT 1 cut(s) 532
SduI GDGCHC 8 cut(s) 382, 551, 569, 605, 623, 674, 710, 728
SexAI ACCWGGT 1 cut(s) 247
SfuI TTCGAA 1 cut(s) 765
SinI GGWCC 3 cut(s) 200, 359, 425
Sse9I AATT 1 cut(s) 446
SsiI CCGC 5 cut(s) 188, 308, 347, 642, 837
SspMI CTAG 4 cut(s) 87, 119, 492, 856
SstI GAGCTC 1 cut(s) 382
StyI CCWWGG 1 cut(s) 773
TaaI ACNGT 1 cut(s) 391
TaqI TCGA 3 cut(s) 416, 431, 765
TasI AATT 1 cut(s) 446
TatI WGTACW 2 cut(s) 386, 530
TfiI GAWTC 1 cut(s) 762
TseI GCWGC 3 cut(s) 593, 644, 791
TspDTI ATGAA 2 cut(s) 17, 839
Van91I CCANNNNNTGG 1 cut(s) 632
VpaK11BI GGWCC 3 cut(s) 200, 359, 425
XapI RAATTY 1 cut(s) 446
XceI RCATGY 1 cut(s) 853
XmiI GTMKAC 1 cut(s) 799
XmnI GAANNNNTTC 2 cut(s) 8, 761
XspI CTAG 4 cut(s) 87, 119, 492, 856
ZrmI AGTACT 1 cut(s) 532
Zsp2I ATGCAT 1 cut(s) 824
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.