pycom02g04850

CRS2-associated factor 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0013996
Physical Location & Seq
Reverse (-)
3155560 .. 3158446
2887 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g04850.2

Sequence Viewer

Length: 2322 bp
ATGGCGCTAAATTTGCCCACTTCCTGTCCCATTTTCGCGCCACTATTGAACCCGAGCCCTACCCACAACCCGGCCCAGCACCGCCCGCCAACGGAGGTCCGATTCGCGCGGTGGAACAACGCCAACGCCGAGAAATTCAACCAGCGCCGGAGGGCCCAGCAAGAAATCGAGGACGATATTCGCCGGGAGCGCCGATTCGAGTCTGCCACCAGAATTGCCACTCTTTCCGACTCTGCCACTGACACCGCCGCCGCCGCCAACGCCACCACGAGTAGTGAAACATTTAAATCCATCGGCACGCCGTCGTATCCCTCGAGCCCTTCAATCCCAGGCAAGAAATCCAAGTACTCCAAAAACCCTAACCCTAACGATTCGCACCCGGCATTTCGCCGAATCATAAGACCCACGAAGGTCTCGAAAATTTTGAGGGAGAAAAAGCCGGCAGTCGACAGAAAAGCCGACATTTCCATCGGCGACGACGGCCTTTCTTACGTCATCGACGGAGCCCCTTTTGAATTCAAGTACAGCTACACCGAGACTCCTAAACTCAAGCCCATTAAGCTCCGGGAACCGCCGTTCGTGCCATTTGGGCCCACCACGATGGACCGGCCATGGACGGGTCGGGCCCCCTTGCCAGCAAGCAAGAAGAAACTGAAGGAATTCGACTCTTTCCAGCTGCCTCCGCCGCACAAGAAGGGGGTCAAGCCGGTGCAATCTCCGGGTCCATATTTGGCCGGGTCGGGTCCTAAGTATGTGAAGTCCAGAGAAGAGATTTTGGGGGAGCCGCTGACAGTTGAGGAAGTTAAGGAATTAGTTAAAGGGTGCATCAAAACCAAGAGGCAGTTGAATATGGGTAGAGATGGTTTGACCCACAACATGTTGGATAACATACATGCTCACTGGAAGCGCCGGAGAGTGTGCAAGATTAAATGCAAAGGTGTTTGTACAGTCGATATGGAGAACGTGTGCGAGCAATTAGAGGAAAGAACTGGAGGGAAGATCATTTACAGGAAAGGTGGAGTGATATACCTTTTCCGAGGCAGAAACTACAACTATAAAACTCGTCCTAAGTTTCCTCTCATGCTTTGGAGACCTATCACTCCTGTATATCCTAGACTGGTTCAGCGGGCTCCCGAGGGTCTAACAGTAGAAGAGGCATCTGAGATGCGTAAGAAGGGACGGAATCTGATACCAATATGCAAGCTTGGGAAAAATGGTGTGTACTCTGAATTGGTAAAAAATGTCAGAGAGGCCTTCGAAGAGTGTGAGCTAGTTCGAATAAATTGTCAGGGGATGAATGGGAGTGACTACCGAAAAATAGGTGCTAAACTTAAGGATCTTGTCCCTTGTGTGTTAATGTCATTTGAACTTGAGCACATTCTTTTGTGGAGAGGACGGGAATGGAAGTCTTCTCTTCCAAATCTAGGGAGTGATCTCAAGGAAGTCAACGAATCTTATGTTGATGATTCAACTTCAATTGCATCAACATCATGTACTTCTGAAGTTGCGGGGGCAGAGGGAAGTGAGGATCTCTCGCCATCACTATATGTTGAGCCACATGCCACAGTTGATGGGGCTTCTACCGTTGGAGGAACCTCTGAAAACAAACCCATTTCAGATGTTGAAGGCTATATAAACAATGAATCAGAAGCTAAAATGAATGCCAGTAACAGCTCAACATTGCAAGACAATATCCACTGTGCTGATGATAAGTCAAAAACTACGCCGCTTACATCTGAAATTGATCCCATGTTGGCTAAAGCAGGCTGTGCTGATGAAGCATCACCAACTGCAGTAATGGGGTCTGGGACCATGGCAGTGCCTGTTGGAAGCGCCGAAACCAGGTTGGATTCCATAACGGTAGGTCCTGGAAGTGACGAGAATCCTCAAGAAGCTTCAGTAGGCTCACAAATTCATAGTGAGCCAGCAAAATTGAGTGCACCTTGTACAGAAAATGTGCTGTTACTCTTGAATGAAGCTGTTGGTAGTGGGAGTGCACTTATTTTAGATGAGTCATCCCTGGATGCCGATATTATTTATCAACGGTCAGTTGCCTTAGCGCAGTCTGCTCCCCCTGGCCCGGTGTTTAAGCATCAACGACCCAAGAAAGTAGCAGTTTTAAAGAGAGTAAAGGTTGTGAAACAAGATGCTGGGGATTCAGAAGTGAAAGAGATAACAGTTTCTGTGAAGAGAGGAAGTGAGAAGATTCAGAAGAAGGGTAGTAAAGTTAAAATGACAAGAGATTTTGGTGAAAATATACTAGATAATGTTGTACCACAGGGAAGTTTGAGAGTTGATGAACTTGCTAAGCTATTAGCATGA

Protein Analysis

774

Amino Acids

84.98

Weight (kDa)

9.05

Isoelectric Point (pI)

49.68

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013996)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G20020
fragaria_vesca FvH4_1g05780
malus_domestica MD02G1059500.v1.1 MD15G1193500.v1.1
prunus_persica Prupe.7G223600_v2.0.a1
pyrus_communis pycom02g04850 pycom15g17320
rosa_chinensis RchiOBHm_Chr2g0091431
rosa_laevigata RLG00000016223
rosa_multiflora Rmu_ssc0000333.1_g000008
rosa_roxburghii Rroxscaffold_2G00150090
rosa_rugosa Rorug02G0017800
rosa_samantha Rh2AG062700 Rh2BG062000 Rh2CG063900 Rh2DG062300
rosa_wichuraiana Rw2G005740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 447
AccII CGCG 3 cut(s) 38, 107, 109
AclWI GGATC 3 cut(s) 1344, 1536, 1739
AcoI YGGCCR 2 cut(s) 608, 732
AcsI RAATTY 6 cut(s) 10, 134, 420, 515, 659, 1911
AcuI CTGAAG 3 cut(s) 674, 1521, 1881
AfaI GTAC 7 cut(s) 347, 524, 946, 1223, 1495, 1948, 2274
AfiI CCNNNNNNNGG 6 cut(s) 70, 91, 617, 1424, 1842, 2080
AflII CTTAAG 1 cut(s) 1331
AflIII ACRYGT 2 cut(s) 876, 963
AjnI CCWGG 5 cut(s) 328, 1841, 1867, 2019, 2074
AloI GAACNNNNNNTCC 2 cut(s) 560, 592
Alw21I GWGCWC 3 cut(s) 1377, 1942, 1999
Alw26I GTCTC 3 cut(s) 418, 530, 1084
Alw44I GTGCAC 2 cut(s) 1938, 1995
AlwI GGATC 3 cut(s) 1344, 1536, 1739
AlwNI CAGNNNCTG 2 cut(s) 1823, 2183
Ama87I CYCGRG 3 cut(s) 52, 313, 1133
AoxI GGCC 9 cut(s) 72, 153, 481, 590, 608, 624, 732, 1251, 2077
ApaI GGGCCC 3 cut(s) 157, 594, 628
ApaLI GTGCAC 2 cut(s) 1938, 1995
ApeKI GCWGC 1 cut(s) 676
ApoI RAATTY 6 cut(s) 10, 134, 420, 515, 659, 1911
ArsI GACNNNNNNTTYG 2 cut(s) 1270, 1302
Asp700I GAANNNNTTC 1 cut(s) 659
AspLEI GCGC 8 cut(s) 7, 40, 109, 147, 192, 909, 1835, 2062
AsuC2I CCSGG 7 cut(s) 71, 185, 380, 566, 720, 736, 2081
AsuHPI GGTGA 2 cut(s) 1776, 2261
AsuII TTCGAA 2 cut(s) 1257, 1276
AvaI CYCGRG 3 cut(s) 52, 313, 1133
AvaII GGWCC 6 cut(s) 97, 604, 722, 743, 1809, 1865
BaeGI GKGCMC 5 cut(s) 157, 594, 628, 1942, 1999
BanII GRGCYC 7 cut(s) 59, 157, 320, 508, 594, 628, 1132
BarI GAAGNNNNNNTAC 2 cut(s) 989, 1021
BauI CACGAG 1 cut(s) 268
BbsI GAAGAC 1 cut(s) 1401
Bbv12I GWGCWC 3 cut(s) 1377, 1942, 1999
BbvI GCAGC 1 cut(s) 663
BccI CCATC 6 cut(s) 299, 476, 595, 854, 1546, 1565
BceAI ACGGC 3 cut(s) 286, 496, 559
BciT130I CCWGG 5 cut(s) 330, 1843, 1869, 2021, 2076
BciVI GTATCC 1 cut(s) 318
BcnI CCSGG 7 cut(s) 71, 185, 380, 566, 720, 736, 2081
BcoDI GTCTC 3 cut(s) 418, 530, 1084
BfaI CTAG 4 cut(s) 1113, 1271, 1424, 2261
BfmI CTRYAG 1 cut(s) 1791
BfoI RGCGCY 5 cut(s) 8, 148, 193, 910, 1836
BfrI CTTAAG 1 cut(s) 1331
BfuI GTATCC 1 cut(s) 318
BglI GCCNNNNNGGC 1 cut(s) 589
BisI GCNGC 7 cut(s) 249, 252, 255, 677, 686, 785, 1727
BlpI GCTNAGC 1 cut(s) 2307
BlsI GCNGC 7 cut(s) 250, 253, 256, 678, 687, 786, 1728
BmcAI AGTACT 1 cut(s) 347
Bme18I GGWCC 6 cut(s) 97, 604, 722, 743, 1809, 1865
BmeT110I CYCGRG 3 cut(s) 52, 313, 1133
BmsI GCATC 8 cut(s) 834, 1155, 1166, 1490, 1790, 2014, 2101, 2137
BpiI GAAGAC 1 cut(s) 1401
BplI GAGNNNNNCTC 2 cut(s) 1517, 1549
BpmI CTGGAG 1 cut(s) 1011
Bpu10I CCTNAGC 1 cut(s) 2056
Bpu1102I GCTNAGC 1 cut(s) 2307
Bpu14I TTCGAA 2 cut(s) 1257, 1276
BpuEI CTTGAG 4 cut(s) 533, 1391, 1421, 1872
BpuMI CCSGG 7 cut(s) 71, 185, 380, 566, 720, 736, 2081
BsaI GGTCTC 2 cut(s) 418, 1084
BsaJI CCNNGG 7 cut(s) 328, 611, 1036, 1134, 1812, 2019, 2074
BsaXI ACNNNNNCTCC 4 cut(s) 523, 553, 950, 980
Bsc4I CCNNNNNNNGG 6 cut(s) 70, 91, 617, 1424, 1842, 2080
Bse118I RCCGGY 3 cut(s) 439, 606, 706
Bse1I ACTGG 4 cut(s) 905, 994, 1122, 1665
Bse3DI GCAATG 1 cut(s) 1679
BseBI CCWGG 5 cut(s) 330, 1843, 1869, 2021, 2076
BseDI CCNNGG 7 cut(s) 328, 611, 1036, 1134, 1812, 2019, 2074
BseGI GGATG 3 cut(s) 1299, 2015, 2029
BseLI CCNNNNNNNGG 6 cut(s) 70, 91, 617, 1424, 1842, 2080
BseMI GCAATG 1 cut(s) 1679
BseMII CTCAG 1 cut(s) 1152
BseNI ACTGG 4 cut(s) 905, 994, 1122, 1665
BseSI GKGCMC 5 cut(s) 157, 594, 628, 1942, 1999
BseXI GCAGC 1 cut(s) 663
BseYI CCCAGC 3 cut(s) 75, 156, 2150
Bsh1236I CGCG 3 cut(s) 38, 107, 109
BshFI GGCC 9 cut(s) 74, 155, 483, 592, 610, 626, 734, 1253, 2079
BsiHKAI GWGCWC 3 cut(s) 1377, 1942, 1999
BsiHKCI CYCGRG 3 cut(s) 52, 313, 1133
BslFI GGGAC 4 cut(s) 12, 1191, 1328, 1822
BslI CCNNNNNNNGG 6 cut(s) 70, 91, 617, 1424, 1842, 2080
BsmAI GTCTC 3 cut(s) 418, 530, 1084
BsmFI GGGAC 4 cut(s) 12, 1191, 1328, 1822
BsmI GAATGC 1 cut(s) 1666
BsnI GGCC 9 cut(s) 74, 155, 483, 592, 610, 626, 734, 1253, 2079
Bso31I GGTCTC 2 cut(s) 418, 1084
BsoBI CYCGRG 3 cut(s) 52, 313, 1133
Bsp119I TTCGAA 2 cut(s) 1257, 1276
Bsp120I GGGCCC 3 cut(s) 153, 590, 624
Bsp1407I TGTACA 2 cut(s) 944, 1946
Bsp143I GATC 5 cut(s) 999, 1336, 1432, 1528, 1744
Bsp1720I GCTNAGC 1 cut(s) 2307
Bsp19I CCATGG 2 cut(s) 611, 1812
BspANI GGCC 9 cut(s) 74, 155, 483, 592, 610, 626, 734, 1253, 2079
BspCNI CTCAG 1 cut(s) 1153
BspFNI CGCG 3 cut(s) 38, 107, 109
BspMAI CTGCAG 1 cut(s) 1795
BspPI GGATC 3 cut(s) 1344, 1536, 1739
BspT104I TTCGAA 2 cut(s) 1257, 1276
BspTI CTTAAG 1 cut(s) 1331
BspTNI GGTCTC 2 cut(s) 418, 1084
BsrDI GCAATG 1 cut(s) 1679
BsrFI RCCGGY 3 cut(s) 439, 606, 706
BsrGI TGTACA 2 cut(s) 944, 1946
BsrI ACTGG 4 cut(s) 905, 994, 1122, 1665
BssAI RCCGGY 3 cut(s) 439, 606, 706
BssECI CCNNGG 7 cut(s) 328, 611, 1036, 1134, 1812, 2019, 2074
BssMI GATC 5 cut(s) 999, 1336, 1432, 1528, 1744
BssSI CACGAG 1 cut(s) 268
BssT1I CCWWGG 2 cut(s) 611, 1812
Bst2BI CACGAG 1 cut(s) 268
Bst2UI CCWGG 5 cut(s) 330, 1843, 1869, 2021, 2076
Bst4CI ACNGT 9 cut(s) 793, 949, 1147, 1567, 1585, 1700, 1861, 2046, 2179
Bst6I CTCTTC 5 cut(s) 762, 1146, 1254, 1419, 2183
BstAFI CTTAAG 1 cut(s) 1331
BstAPI GCANNNNNTGC 1 cut(s) 1769
BstAUI TGTACA 2 cut(s) 944, 1946
BstBI TTCGAA 2 cut(s) 1257, 1276
BstDEI CTNAG 5 cut(s) 747, 1068, 1161, 2056, 2307
BstDSI CCRYGG 2 cut(s) 611, 1812
BstF5I GGATG 3 cut(s) 1299, 2015, 2029
BstFNI CGCG 3 cut(s) 38, 107, 109
BstH2I RGCGCY 5 cut(s) 8, 148, 193, 910, 1836
BstHHI GCGC 8 cut(s) 7, 40, 109, 147, 192, 909, 1835, 2062
BstKTI GATC 5 cut(s) 1002, 1339, 1435, 1531, 1747
BstMAI GTCTC 3 cut(s) 418, 530, 1084
BstMBI GATC 5 cut(s) 999, 1336, 1432, 1528, 1744
BstNI CCWGG 5 cut(s) 330, 1843, 1869, 2021, 2076
BstNSI RCATGY 3 cut(s) 880, 896, 1562
BstSFI CTRYAG 1 cut(s) 1791
BstSLI GKGCMC 5 cut(s) 157, 594, 628, 1942, 1999
BstUI CGCG 3 cut(s) 38, 107, 109
BstV1I GCAGC 1 cut(s) 663
BstV2I GAAGAC 1 cut(s) 1401
BstX2I RGATCY 2 cut(s) 1336, 1528
BstXI CCANNNNNNTGG 1 cut(s) 601
BstYI RGATCY 2 cut(s) 1336, 1528
BsuI GTATCC 1 cut(s) 318
BsuRI GGCC 9 cut(s) 74, 155, 483, 592, 610, 626, 734, 1253, 2079
BtgI CCRYGG 2 cut(s) 611, 1812
BtsCI GGATG 3 cut(s) 1299, 2015, 2029
BtsI GCAGTG 1 cut(s) 1824
BtsIMutI CAGTG 4 cut(s) 237, 898, 1696, 1824
CaiI CAGNNNCTG 2 cut(s) 1823, 2183
CfoI GCGC 8 cut(s) 7, 40, 109, 147, 192, 909, 1835, 2062
Cfr10I RCCGGY 3 cut(s) 439, 606, 706
CsiI ACCWGGT 1 cut(s) 1841
Csp6I GTAC 7 cut(s) 346, 523, 945, 1222, 1494, 1947, 2273
CspCI CAANNNNNGTGG 2 cut(s) 196, 231
CviAII CATG 9 cut(s) 612, 877, 893, 1081, 1491, 1559, 1750, 1813, 2319
CviQI GTAC 7 cut(s) 346, 523, 945, 1222, 1494, 1947, 2273
DdeI CTNAG 5 cut(s) 747, 1068, 1161, 2056, 2307
DpnI GATC 5 cut(s) 1001, 1338, 1434, 1530, 1746
DpnII GATC 5 cut(s) 999, 1336, 1432, 1528, 1744
DraI TTTAAA 2 cut(s) 286, 2121
EaeI YGGCCR 2 cut(s) 608, 732
Eam1104I CTCTTC 5 cut(s) 762, 1146, 1254, 1419, 2183
EarI CTCTTC 5 cut(s) 762, 1146, 1254, 1419, 2183
EciI GGCGGA 1 cut(s) 672
Eco130I CCWWGG 2 cut(s) 611, 1812
Eco147I AGGCCT 1 cut(s) 1253
Eco24I GRGCYC 7 cut(s) 59, 157, 320, 508, 594, 628, 1132
Eco31I GGTCTC 2 cut(s) 418, 1084
Eco47I GGWCC 6 cut(s) 97, 604, 722, 743, 1809, 1865
Eco57I CTGAAG 3 cut(s) 674, 1521, 1881
Eco88I CYCGRG 3 cut(s) 52, 313, 1133
EcoO109I RGGNCCY 4 cut(s) 153, 625, 743, 1865
EcoRI GAATTC 2 cut(s) 515, 659
EcoRII CCWGG 5 cut(s) 328, 1841, 1867, 2019, 2074
EcoT14I CCWWGG 2 cut(s) 611, 1812
EcoT38I GRGCYC 7 cut(s) 59, 157, 320, 508, 594, 628, 1132
ErhI CCWWGG 2 cut(s) 611, 1812
FaeI CATG 9 cut(s) 615, 880, 896, 1084, 1494, 1562, 1753, 1816, 2322
FaqI GGGAC 4 cut(s) 12, 1191, 1328, 1822
FatI CATG 9 cut(s) 611, 876, 892, 1080, 1490, 1558, 1749, 1812, 2318
FauI CCCGC 3 cut(s) 93, 1119, 1501
FblI GTMKAC 1 cut(s) 447
Fnu4HI GCNGC 7 cut(s) 249, 252, 255, 677, 686, 785, 1727
FokI GGATG 3 cut(s) 1306, 2002, 2036
FriOI GRGCYC 7 cut(s) 59, 157, 320, 508, 594, 628, 1132
Fsp4HI GCNGC 7 cut(s) 249, 252, 255, 677, 686, 785, 1727
FspBI CTAG 4 cut(s) 1113, 1271, 1424, 2261
GlaI GCGC 8 cut(s) 6, 39, 108, 146, 191, 908, 1834, 2061
GluI GCNGC 7 cut(s) 249, 252, 255, 677, 686, 785, 1727
GsaI CCCAGC 3 cut(s) 79, 160, 2154
GsuI CTGGAG 1 cut(s) 1011
HaeII RGCGCY 5 cut(s) 8, 148, 193, 910, 1836
HaeIII GGCC 9 cut(s) 74, 155, 483, 592, 610, 626, 734, 1253, 2079
HhaI GCGC 8 cut(s) 7, 40, 109, 147, 192, 909, 1835, 2062
Hin1II CATG 9 cut(s) 615, 880, 896, 1084, 1494, 1562, 1753, 1816, 2322
Hin6I GCGC 8 cut(s) 5, 38, 107, 145, 190, 907, 1833, 2060
HinP1I GCGC 8 cut(s) 5, 38, 107, 145, 190, 907, 1833, 2060
HincII GTYRAC 2 cut(s) 448, 1447
HindII GTYRAC 2 cut(s) 448, 1447
HindIII AAGCTT 2 cut(s) 1202, 1893
HphI GGTGA 2 cut(s) 1776, 2261
Hpy166II GTNNAC 5 cut(s) 448, 1222, 1447, 1940, 1997
Hpy188III TCNNGA 5 cut(s) 415, 762, 1133, 1889, 1969
Hpy8I GTNNAC 5 cut(s) 448, 1222, 1447, 1940, 1997
Hpy99I CGWCG 4 cut(s) 307, 479, 482, 503
HpyAV CCTTC 8 cut(s) 330, 403, 649, 688, 1168, 1264, 1619, 2209
HpyCH4III ACNGT 9 cut(s) 793, 949, 1147, 1567, 1585, 1700, 1861, 2046, 2179
HpyCH4IV ACGT 2 cut(s) 492, 963
HpyF3I CTNAG 5 cut(s) 747, 1068, 1161, 2056, 2307
HpySE526I ACGT 2 cut(s) 492, 963
Hsp92II CATG 9 cut(s) 615, 880, 896, 1084, 1494, 1562, 1753, 1816, 2322
HspAI GCGC 8 cut(s) 5, 38, 107, 145, 190, 907, 1833, 2060
KroI GCCGGC 1 cut(s) 439
KroNI GCCGGC 1 cut(s) 441
Kzo9I GATC 5 cut(s) 999, 1336, 1432, 1528, 1744
LmnI GCTCC 6 cut(s) 187, 503, 567, 781, 1135, 2074
Lsp1109I GCAGC 1 cut(s) 663
LweI GCATC 8 cut(s) 834, 1155, 1166, 1490, 1790, 2014, 2101, 2137
MabI ACCWGGT 1 cut(s) 1841
MaeI CTAG 4 cut(s) 1113, 1271, 1424, 2261
MaeII ACGT 2 cut(s) 492, 963
MaeIII GTNAC 4 cut(s) 1304, 1667, 1874, 1962
MalI GATC 5 cut(s) 1001, 1338, 1434, 1530, 1746
MboI GATC 5 cut(s) 999, 1336, 1432, 1528, 1744
MfeI CAATTG 1 cut(s) 1476
MflI RGATCY 2 cut(s) 1336, 1528
MlyI GAGTC 5 cut(s) 209, 224, 532, 659, 2021
MmeI TCCRAC 5 cut(s) 252, 861, 1567, 1807, 1827
MroNI GCCGGC 1 cut(s) 439
MroXI GAANNNNTTC 1 cut(s) 659
MslI CAYNNNNRTG 2 cut(s) 599, 1817
MspA1I CMGCKG 3 cut(s) 676, 787, 1126
MspCI CTTAAG 1 cut(s) 1331
MunI CAATTG 1 cut(s) 1476
Mva1269I GAATGC 1 cut(s) 1666
MvaI CCWGG 5 cut(s) 330, 1843, 1869, 2021, 2076
MvnI CGCG 3 cut(s) 38, 107, 109
NaeI GCCGGC 1 cut(s) 441
NciI CCSGG 7 cut(s) 71, 185, 380, 566, 720, 736, 2081
NcoI CCATGG 2 cut(s) 611, 1812
NdeII GATC 5 cut(s) 999, 1336, 1432, 1528, 1744
NgoMIV GCCGGC 1 cut(s) 439
NlaIII CATG 9 cut(s) 615, 880, 896, 1084, 1494, 1562, 1753, 1816, 2322
NmeAIII GCCGAG 1 cut(s) 154
NmuCI GTSAC 2 cut(s) 1304, 1874
NspI RCATGY 3 cut(s) 880, 896, 1562
NspV TTCGAA 2 cut(s) 1257, 1276
PaeR7I CTCGAG 1 cut(s) 313
PceI AGGCCT 1 cut(s) 1253
PciI ACATGT 1 cut(s) 876
PcsI WCGNNNNNNNCGW 3 cut(s) 126, 311, 413
PctI GAATGC 1 cut(s) 1666
PdiI GCCGGC 1 cut(s) 441
PdmI GAANNNNTTC 1 cut(s) 659
PfoI TCCNGGA 2 cut(s) 564, 1867
PkrI GCNGC 7 cut(s) 250, 253, 256, 678, 687, 786, 1728
PleI GAGTC 5 cut(s) 208, 224, 532, 659, 2020
PpsI GAGTC 5 cut(s) 208, 224, 532, 659, 2020
PpuMI RGGWCCY 2 cut(s) 743, 1865
PscI ACATGT 1 cut(s) 876
Psp5II RGGWCCY 2 cut(s) 743, 1865
Psp6I CCWGG 5 cut(s) 328, 1841, 1867, 2019, 2074
PspFI CCCAGC 3 cut(s) 75, 156, 2150
PspGI CCWGG 5 cut(s) 328, 1841, 1867, 2019, 2074
PspOMI GGGCCC 3 cut(s) 153, 590, 624
PspPPI RGGWCCY 2 cut(s) 743, 1865
PspXI VCTCGAGB 1 cut(s) 313
PstI CTGCAG 1 cut(s) 1795
PstNI CAGNNNCTG 2 cut(s) 1823, 2183
PsuI RGATCY 2 cut(s) 1336, 1528
PvuII CAGCTG 1 cut(s) 676
RsaI GTAC 7 cut(s) 347, 524, 946, 1223, 1495, 1948, 2274
RsaNI GTAC 7 cut(s) 346, 523, 945, 1222, 1494, 1947, 2273
RseI CAYNNNNRTG 2 cut(s) 599, 1817
SalI GTCGAC 1 cut(s) 446
SatI GCNGC 7 cut(s) 249, 252, 255, 677, 686, 785, 1727
Sau3AI GATC 5 cut(s) 999, 1336, 1432, 1528, 1744
ScaI AGTACT 1 cut(s) 347
SchI GAGTC 5 cut(s) 209, 224, 532, 659, 2021
SexAI ACCWGGT 1 cut(s) 1841
SfaNI GCATC 8 cut(s) 834, 1155, 1166, 1490, 1790, 2014, 2101, 2137
SfcI CTRYAG 1 cut(s) 1791
Sfr274I CTCGAG 1 cut(s) 313
SfuI TTCGAA 2 cut(s) 1257, 1276
SinI GGWCC 6 cut(s) 97, 604, 722, 743, 1809, 1865
SlaI CTCGAG 1 cut(s) 313
SmiI ATTTAAAT 1 cut(s) 286
SmiMI CAYNNNNRTG 2 cut(s) 599, 1817
SmlI CTYRAG 6 cut(s) 313, 548, 1331, 1370, 1436, 1887
SmoI CTYRAG 6 cut(s) 313, 548, 1331, 1370, 1436, 1887
SseBI AGGCCT 1 cut(s) 1253
SspMI CTAG 4 cut(s) 1113, 1271, 1424, 2261
StuI AGGCCT 1 cut(s) 1253
StyI CCWWGG 2 cut(s) 611, 1812
SwaI ATTTAAAT 1 cut(s) 286
TaaI ACNGT 9 cut(s) 793, 949, 1147, 1567, 1585, 1700, 1861, 2046, 2179
TaiI ACGT 2 cut(s) 495, 966
TatI WGTACW 6 cut(s) 345, 522, 944, 1221, 1493, 1946
TauI GCSGC 6 cut(s) 251, 254, 257, 688, 787, 1729
TscAI CASTG 4 cut(s) 244, 905, 1703, 1824
TseFI GTSAC 2 cut(s) 1304, 1874
TseI GCWGC 1 cut(s) 676
Tsp45I GTSAC 2 cut(s) 1304, 1874
TspDTI ATGAA 7 cut(s) 1310, 1656, 1673, 1791, 1904, 1989, 2313
TspGWI ACGGA 3 cut(s) 107, 516, 1195
TspRI CASTG 4 cut(s) 244, 905, 1703, 1824
Vha464I CTTAAG 1 cut(s) 1331
VneI GTGCAC 2 cut(s) 1938, 1995
VpaK11BI GGWCC 6 cut(s) 97, 604, 722, 743, 1809, 1865
XapI RAATTY 6 cut(s) 10, 134, 420, 515, 659, 1911
XceI RCATGY 3 cut(s) 880, 896, 1562
XhoI CTCGAG 1 cut(s) 313
XmiI GTMKAC 1 cut(s) 447
XmnI GAANNNNTTC 1 cut(s) 659
XspI CTAG 4 cut(s) 1113, 1271, 1424, 2261
ZrmI AGTACT 1 cut(s) 347
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.