Rmu_ssc0000333.1_g000008

CRS2-associated factor 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000333.1
OG0013996
Physical Location & Seq
Forward (+)
31383 .. 34400
3018 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_ssc0000333.1_g000008.1.cds

Sequence Viewer

Length: 2496 bp
atggcgctaaatttgccgacttcgtgccccattttcgcgccaccggtgaacccgaacccggcccacaacccgctccagacccgcccgcccacggaggtccgattcgcgcggtggaacaacgccaacgccgagaaattcaaccagcgccgccgcgcccagcaagaaatcgaggacgacgtccgccgcgagcgccgcttcgactccgccaccagaatcgccaccgtcgccgccgacgactcctcctcctccgacgccgaaccaacattcaaatccataggcacgccttcgtctccctcaagcccttcaattcccggcagaaaatccaagtactccaagaaccctaaccctagctcgcacccggcgtttcgccgcgtcatcagacccacgaagctctcgagtatcgccagagagaagccggaaatcgaccggaaagctaacatctccgtcggcgacgacgggctttcgtacgtcatcgacggcgcgccgtttgagttcaagtacagctacaccgagaccccgaagcagaagcccattaagctccgggagccgccctacacgccgttcggccccaccacgatgggccggccgtggacgggtcgggccccattgcctccgagcaagaagaaactgaaggagttcgattcgttccagctaccgccgccgcataagaaaggggttaagccggtgcagtcgccgggtccgtatttacccgggtcgggtcccaagtatgtcaagtccagggaggagatattgggtgagccgttgaccgatcaggaagtcaaagacttggttaatagctgtgttaagaccagaaggcaactcaatatgggtagagatggattgacacacaacatgctggataatattcatgctcattggaagcggcggagagtgtgcaagataaaatgtaaaggggtgtgtacagtcgacatggaaaatgtgtgccagcaattagaggaaagaactggagggaaaatcatttatagaagaggtggggtgatttttcttttccgggggagaaactacaactataaaactcggccgcggtttcctctcatgctttggagacctatcactcctgtgtatcctagattgattcagagggctcctgagggactaacagttgaagaagcaactgaaatgcgcaagaaggggaggaatctgataccaatacgcaagctaggaaaaaatggagtatactctgacctggtagacaatgtcagagaggcctttgaagagtgtgaactagtgcgaatagattgtcaagggatgaatgggagtgactaccgtaaaataggtgctaaactaaaggatcttgtcccttgtgtgctaatttcctttgaacgcgagaacattcttgtgtggagaggacgggaatggaagtcttctttcctaaatccggagagtaatcttaaggaagtcaaagaatctgatgttgatgattcaccttctattgcaccttccttggaaggtgaagaagcatcgacgttatgtgcttttacagcttcagtcaatgacgaaaatcctcaaatgatcgacactagtatcacctcttcaagttctgaagttgtgggtgctgagggaactgaggatccatccccttcaccatatattgagccatgtgccacaattgatacagtttccactgtgggaggcacttatgaaactgtaaccatctcggacatcaaaggctttagaaatgatgaagcagaattggagatgaaggcctatagcagttcaaccataccacaggataccaggtatgctcatgatgaggcagaaacgatttccatctcagacatcaagggctttagaaatgatgaagcagaattggaaatgaaggcctatagcagttcaaccttaccgcaggatgctgatgatgaggccgaaacaatttccagtacatctggaactgaaaacatattggatagtacatgccatactgatgaagcatcagcaactacatcagtggggaccgggaccatgccagtgactgttgaaagcaccgaaacgaagttggattccctcatggttgcccctggtagtaatgagactccacaagatgcttgtatagactcgcaaaatctcaatgagcaggcaaggttgagtgcagcctgtaaagagaaggtgctatcactcttgaacgaagctgttgggagtgggagtgcacttattttagacgattcgtcgttggatgctgacatcatatatcagagggcagttgctctcgccaagtctgctccacctgggccagtgtttaagcatagacctaggagagttgcagctcaaatgagtaagaagctagtagtaagaaaacagaagcaagaagccactgagttggaagtgaaagagataacagtttatgcaaagaagggaagtgagaagaaggattccaaagttcacagaacaaaaacaagagattttggtgaacgtcttgatagcattgtaccacagggaagcctgagagttgatgaacttgctaagctattagcctga

Protein Analysis

831

Amino Acids

92.19

Weight (kDa)

7.8

Isoelectric Point (pI)

52.36

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013996)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G20020
fragaria_vesca FvH4_1g05780
malus_domestica MD02G1059500.v1.1 MD15G1193500.v1.1
prunus_persica Prupe.7G223600_v2.0.a1
pyrus_communis pycom02g04850 pycom15g17320
rosa_chinensis RchiOBHm_Chr2g0091431
rosa_laevigata RLG00000016223
rosa_multiflora Rmu_ssc0000333.1_g000008
rosa_roxburghii Rroxscaffold_2G00150090
rosa_rugosa Rorug02G0017800
rosa_samantha Rh2AG062700 Rh2BG062000 Rh2CG063900 Rh2DG062300
rosa_wichuraiana Rw2G005740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 180
Acc16I TGCGCA 1 cut(s) 1145
AccBSI CCGCTC 1 cut(s) 73
AccI GTMKAC 3 cut(s) 927, 1197, 1212
AccII CGCG 9 cut(s) 38, 107, 109, 153, 186, 372, 482, 1045, 1347
AccIII TCCGGA 1 cut(s) 1399
AclWI GGATC 3 cut(s) 1320, 1586, 1599
AcoI YGGCCR 2 cut(s) 584, 1040
AcsI RAATTY 2 cut(s) 10, 134
AcuI CTGAAG 3 cut(s) 650, 1491, 1584
AcyI GRCGYC 2 cut(s) 177, 252
AfaI GTAC 7 cut(s) 329, 467, 500, 922, 1903, 1933, 2448
AfiI CCNNNNNNNGG 5 cut(s) 58, 91, 593, 716, 1399
AflII CTTAAG 1 cut(s) 1412
AgeI ACCGGT 1 cut(s) 43
AhdI GACNNNNNGTC 1 cut(s) 2185
AhlI ACTAGT 2 cut(s) 1246, 1541
AjnI CCWGG 5 cut(s) 737, 1206, 1757, 2038, 2245
AleI CACNNNNGTG 1 cut(s) 1079
AloI GAACNNNNNNTCC 2 cut(s) 1576, 1608
Alw21I GWGCWC 1 cut(s) 2170
Alw26I GTCTC 4 cut(s) 294, 506, 1060, 2045
Alw44I GTGCAC 1 cut(s) 2166
AlwI GGATC 3 cut(s) 1320, 1586, 1599
AlwNI CAGNNNCTG 1 cut(s) 1994
Ama87I CYCGRG 2 cut(s) 394, 710
Aor13HI TCCGGA 1 cut(s) 1399
ApaI GGGCCC 1 cut(s) 604
ApaLI GTGCAC 1 cut(s) 2166
ApeKI GCWGC 2 cut(s) 2111, 2282
ApoI RAATTY 2 cut(s) 10, 134
ArsI GACNNNNNNTTYG 2 cut(s) 1246, 1278
AscI GGCGCGCC 1 cut(s) 480
AsiGI ACCGGT 1 cut(s) 43
Asp700I GAANNNNTTC 1 cut(s) 635
AspA2I CCTAGG 1 cut(s) 2270
AspLEI GCGC 9 cut(s) 7, 40, 109, 147, 155, 192, 482, 484, 1146
AsuC2I CCSGG 9 cut(s) 59, 312, 359, 542, 696, 711, 712, 1013, 1978
AsuHPI GGTGA 8 cut(s) 58, 767, 1009, 1437, 1484, 1540, 1596, 2438
AvaI CYCGRG 2 cut(s) 394, 710
AvaII GGWCC 5 cut(s) 97, 698, 719, 1974, 1980
AvrII CCTAGG 1 cut(s) 2270
AxyI CCTNAGG 1 cut(s) 1110
BaeGI GKGCMC 3 cut(s) 29, 604, 2170
BaeI ACNNNNGTAYC 2 cut(s) 1528, 1561
BamHI GGATCC 1 cut(s) 1591
BanII GRGCYC 2 cut(s) 604, 1108
BarI GAAGNNNNNNTAC 2 cut(s) 1537, 1569
BbsI GAAGAC 1 cut(s) 1377
Bbv12I GWGCWC 1 cut(s) 2170
BbvCI CCTCAGC 1 cut(s) 1578
BbvI GCAGC 2 cut(s) 2123, 2294
BccI CCATC 5 cut(s) 571, 830, 1603, 1682, 1799
BceAI ACGGC 5 cut(s) 469, 493, 544, 571, 745
BciT130I CCWGG 5 cut(s) 739, 1208, 1759, 2040, 2247
BciVI GTATCC 2 cut(s) 1095, 1747
BcnI CCSGG 9 cut(s) 59, 312, 359, 542, 696, 711, 712, 1013, 1978
BcoDI GTCTC 4 cut(s) 294, 506, 1060, 2045
BcuI ACTAGT 2 cut(s) 1246, 1541
BfaI CTAG 7 cut(s) 348, 1089, 1181, 1247, 1542, 2271, 2303
BfmI CTRYAG 2 cut(s) 1729, 1846
BfoI RGCGCY 3 cut(s) 8, 148, 193
BfrI CTTAAG 1 cut(s) 1412
BfuI GTATCC 2 cut(s) 1095, 1747
BlnI CCTAGG 1 cut(s) 2270
BlpI GCTNAGC 1 cut(s) 2481
BmcAI AGTACT 1 cut(s) 329
Bme18I GGWCC 5 cut(s) 97, 698, 719, 1974, 1980
BmeRI GACNNNNNGTC 1 cut(s) 2185
BmeT110I CYCGRG 2 cut(s) 394, 710
BmsI GCATC 5 cut(s) 1490, 1861, 1961, 2053, 2185
BpiI GAAGAC 1 cut(s) 1377
BpmI CTGGAG 2 cut(s) 59, 987
Bpu10I CCTNAGC 1 cut(s) 1578
Bpu1102I GCTNAGC 1 cut(s) 2481
BpuEI CTTGAG 1 cut(s) 280
BpuMI CCSGG 9 cut(s) 59, 312, 359, 542, 696, 711, 712, 1013, 1978
BsaBI GATNNNNATC 1 cut(s) 1790
BsaHI GRCGYC 2 cut(s) 177, 252
BsaI GGTCTC 2 cut(s) 506, 1060
BsaWI WCCGGW 3 cut(s) 43, 426, 1399
BsaXI ACNNNNNCTCC 6 cut(s) 224, 227, 254, 257, 2149, 2179
Bsc4I CCNNNNNNNGG 5 cut(s) 58, 91, 593, 716, 1399
Bse118I RCCGGY 3 cut(s) 43, 582, 682
Bse1I ACTGG 4 cut(s) 970, 1899, 1988, 2252
Bse21I CCTNAGG 1 cut(s) 1110
Bse3DI GCAATG 1 cut(s) 605
Bse8I GATNNNNATC 1 cut(s) 1790
BseAI TCCGGA 1 cut(s) 1399
BseBI CCWGG 5 cut(s) 739, 1208, 1759, 2040, 2247
BseGI GGATG 4 cut(s) 1275, 1595, 1876, 2200
BseJI GATNNNNATC 1 cut(s) 1790
BseLI CCNNNNNNNGG 5 cut(s) 58, 91, 593, 716, 1399
BseMI GCAATG 1 cut(s) 605
BseMII CTCAG 6 cut(s) 1101, 1569, 1578, 1809, 2325, 2453
BseNI ACTGG 4 cut(s) 970, 1899, 1988, 2252
BsePI GCGCGC 1 cut(s) 480
BseRI GAGGAG 4 cut(s) 229, 232, 235, 758
BseSI GKGCMC 3 cut(s) 29, 604, 2170
BseX3I CGGCCG 2 cut(s) 584, 1040
BseXI GCAGC 2 cut(s) 2123, 2294
BseYI CCCAGC 1 cut(s) 156
BsgI GTGCAG 2 cut(s) 707, 2130
Bsh1236I CGCG 9 cut(s) 38, 107, 109, 153, 186, 372, 482, 1045, 1347
Bsh1285I CGRYCG 3 cut(s) 427, 587, 1043
BshTI ACCGGT 1 cut(s) 43
BsiEI CGRYCG 3 cut(s) 427, 587, 1043
BsiHKAI GWGCWC 1 cut(s) 2170
BsiHKCI CYCGRG 2 cut(s) 394, 710
BsiWI CGTACG 1 cut(s) 465
BslFI GGGAC 5 cut(s) 705, 1128, 1304, 1987, 1993
BslI CCNNNNNNNGG 5 cut(s) 58, 91, 593, 716, 1399
BsmAI GTCTC 4 cut(s) 294, 506, 1060, 2045
BsmBI CGTCTC 1 cut(s) 294
BsmFI GGGAC 5 cut(s) 705, 1128, 1304, 1987, 1993
Bso31I GGTCTC 2 cut(s) 506, 1060
BsoBI CYCGRG 2 cut(s) 394, 710
Bsp120I GGGCCC 1 cut(s) 600
Bsp1286I GDGCHC 4 cut(s) 29, 604, 1108, 2170
Bsp13I TCCGGA 1 cut(s) 1399
Bsp1407I TGTACA 1 cut(s) 920
Bsp143I GATC 4 cut(s) 769, 1312, 1533, 1591
Bsp1720I GCTNAGC 1 cut(s) 2481
BspCNI CTCAG 6 cut(s) 1102, 1570, 1579, 1808, 2326, 2454
BspEI TCCGGA 1 cut(s) 1399
BspFNI CGCG 9 cut(s) 38, 107, 109, 153, 186, 372, 482, 1045, 1347
BspHI TCATGA 1 cut(s) 1768
BspPI GGATC 3 cut(s) 1320, 1586, 1599
BspTI CTTAAG 1 cut(s) 1412
BspTNI GGTCTC 2 cut(s) 506, 1060
BsrBI CCGCTC 1 cut(s) 73
BsrDI GCAATG 1 cut(s) 605
BsrFI RCCGGY 3 cut(s) 43, 582, 682
BsrGI TGTACA 1 cut(s) 920
BsrI ACTGG 4 cut(s) 970, 1899, 1988, 2252
BssAI RCCGGY 3 cut(s) 43, 582, 682
BssHII GCGCGC 1 cut(s) 480
BssMI GATC 4 cut(s) 769, 1312, 1533, 1591
BssNAI GTATAC 1 cut(s) 1198
BssNI GRCGYC 2 cut(s) 177, 252
BssT1I CCWWGG 2 cut(s) 1464, 2270
Bst1107I GTATAC 1 cut(s) 1198
Bst2UI CCWGG 5 cut(s) 739, 1208, 1759, 2040, 2247
Bst4CI ACNGT 9 cut(s) 223, 925, 1123, 1289, 1639, 1648, 1669, 1996, 2359
Bst6I CTCTTC 3 cut(s) 982, 1230, 1558
BstACI GRCGYC 2 cut(s) 177, 252
BstAFI CTTAAG 1 cut(s) 1412
BstAUI TGTACA 1 cut(s) 920
BstC8I GCNNGC 9 cut(s) 86, 188, 281, 353, 482, 584, 947, 1178, 2097
BstDEI CTNAG 7 cut(s) 1110, 1578, 1587, 1795, 2334, 2462, 2481
BstDSI CCRYGG 3 cut(s) 90, 587, 1043
BstF5I GGATG 4 cut(s) 1275, 1595, 1876, 2200
BstFNI CGCG 9 cut(s) 38, 107, 109, 153, 186, 372, 482, 1045, 1347
BstH2I RGCGCY 3 cut(s) 8, 148, 193
BstHHI GCGC 9 cut(s) 7, 40, 109, 147, 155, 192, 482, 484, 1146
BstKTI GATC 4 cut(s) 772, 1315, 1536, 1594
BstMAI GTCTC 4 cut(s) 294, 506, 1060, 2045
BstMBI GATC 4 cut(s) 769, 1312, 1533, 1591
BstMCI CGRYCG 3 cut(s) 427, 587, 1043
BstNI CCWGG 5 cut(s) 739, 1208, 1759, 2040, 2247
BstNSI RCATGY 2 cut(s) 856, 1938
BstSFI CTRYAG 2 cut(s) 1729, 1846
BstSLI GKGCMC 3 cut(s) 29, 604, 2170
BstUI CGCG 9 cut(s) 38, 107, 109, 153, 186, 372, 482, 1045, 1347
BstV1I GCAGC 2 cut(s) 2123, 2294
BstV2I GAAGAC 1 cut(s) 1377
BstX2I RGATCY 2 cut(s) 1312, 1591
BstXI CCANNNNNNTGG 2 cut(s) 577, 2338
BstYI RGATCY 2 cut(s) 1312, 1591
BstZ17I GTATAC 1 cut(s) 1198
BstZI CGGCCG 2 cut(s) 584, 1040
Bsu36I CCTNAGG 1 cut(s) 1110
BsuI GTATCC 2 cut(s) 1095, 1747
BtgI CCRYGG 3 cut(s) 90, 587, 1043
BtsCI GGATG 4 cut(s) 1275, 1595, 1876, 2200
BtsIMutI CAGTG 5 cut(s) 1644, 1974, 1995, 2259, 2331
Cac8I GCNNGC 9 cut(s) 86, 188, 281, 353, 482, 584, 947, 1178, 2097
CaiI CAGNNNCTG 1 cut(s) 1994
CciI TCATGA 1 cut(s) 1768
CfoI GCGC 9 cut(s) 7, 40, 109, 147, 155, 192, 482, 484, 1146
Cfr10I RCCGGY 3 cut(s) 43, 582, 682
Cfr42I CCGCGG 1 cut(s) 1046
Cfr9I CCCGGG 1 cut(s) 710
CseI GACGC 2 cut(s) 260, 361
CsiI ACCWGGT 2 cut(s) 1206, 1757
Csp6I GTAC 7 cut(s) 328, 466, 499, 921, 1902, 1932, 2447
CspAI ACCGGT 1 cut(s) 43
CviAII CATG 9 cut(s) 853, 869, 931, 1057, 1620, 1769, 1935, 1984, 2029
CviQI GTAC 7 cut(s) 328, 466, 499, 921, 1902, 1932, 2447
DdeI CTNAG 7 cut(s) 1110, 1578, 1587, 1795, 2334, 2462, 2481
DpnI GATC 4 cut(s) 771, 1314, 1535, 1593
DpnII GATC 4 cut(s) 769, 1312, 1533, 1591
DriI GACNNNNNGTC 1 cut(s) 2185
EaeI YGGCCR 2 cut(s) 584, 1040
EagI CGGCCG 2 cut(s) 584, 1040
Eam1104I CTCTTC 3 cut(s) 982, 1230, 1558
Eam1105I GACNNNNNGTC 1 cut(s) 2185
EarI CTCTTC 3 cut(s) 982, 1230, 1558
EciI GGCGGA 3 cut(s) 170, 193, 901
EclXI CGGCCG 2 cut(s) 584, 1040
Eco130I CCWWGG 2 cut(s) 1464, 2270
Eco147I AGGCCT 3 cut(s) 1229, 1727, 1844
Eco24I GRGCYC 2 cut(s) 604, 1108
Eco31I GGTCTC 2 cut(s) 506, 1060
Eco47I GGWCC 5 cut(s) 97, 698, 719, 1974, 1980
Eco52I CGGCCG 2 cut(s) 584, 1040
Eco57I CTGAAG 3 cut(s) 650, 1491, 1584
Eco81I CCTNAGG 1 cut(s) 1110
Eco88I CYCGRG 2 cut(s) 394, 710
EcoO109I RGGNCCY 2 cut(s) 601, 719
EcoRII CCWGG 5 cut(s) 737, 1206, 1757, 2038, 2245
EcoT14I CCWWGG 2 cut(s) 1464, 2270
EcoT38I GRGCYC 2 cut(s) 604, 1108
ErhI CCWWGG 2 cut(s) 1464, 2270
Esp3I CGTCTC 1 cut(s) 294
FaeI CATG 9 cut(s) 856, 872, 934, 1060, 1623, 1772, 1938, 1987, 2032
FaqI GGGAC 5 cut(s) 705, 1128, 1304, 1987, 1993
FatI CATG 9 cut(s) 852, 868, 930, 1056, 1619, 1768, 1934, 1983, 2028
FauI CCCGC 3 cut(s) 78, 89, 93
FblI GTMKAC 3 cut(s) 927, 1197, 1212
FokI GGATG 4 cut(s) 1282, 1582, 1883, 2207
FriOI GRGCYC 2 cut(s) 604, 1108
FseI GGCCGGCC 1 cut(s) 586
FspBI CTAG 7 cut(s) 348, 1089, 1181, 1247, 1542, 2271, 2303
FspI TGCGCA 1 cut(s) 1145
GlaI GCGC 9 cut(s) 6, 39, 108, 146, 154, 191, 481, 483, 1145
GsaI CCCAGC 1 cut(s) 160
GsuI CTGGAG 2 cut(s) 59, 987
HaeII RGCGCY 3 cut(s) 8, 148, 193
HgaI GACGC 2 cut(s) 260, 361
HhaI GCGC 9 cut(s) 7, 40, 109, 147, 155, 192, 482, 484, 1146
Hin1I GRCGYC 2 cut(s) 177, 252
Hin1II CATG 9 cut(s) 856, 872, 934, 1060, 1623, 1772, 1938, 1987, 2032
Hin6I GCGC 9 cut(s) 5, 38, 107, 145, 153, 190, 480, 482, 1144
HinP1I GCGC 9 cut(s) 5, 38, 107, 145, 153, 190, 480, 482, 1144
HincII GTYRAC 2 cut(s) 765, 928
HindII GTYRAC 2 cut(s) 765, 928
HphI GGTGA 8 cut(s) 58, 767, 1009, 1437, 1484, 1540, 1596, 2438
Hpy188III TCNNGA 9 cut(s) 76, 394, 773, 1109, 1400, 1769, 1908, 2140, 2435
HpyCH4III ACNGT 9 cut(s) 223, 925, 1123, 1289, 1639, 1648, 1669, 1996, 2359
HpyCH4IV ACGT 4 cut(s) 177, 468, 1487, 2431
HpyCH4V TGCA 7 cut(s) 688, 897, 1457, 2111, 2168, 2282, 2366
HpyF3I CTNAG 7 cut(s) 1110, 1578, 1587, 1795, 2334, 2462, 2481
HpySE526I ACGT 4 cut(s) 177, 468, 1487, 2431
Hsp92I GRCGYC 2 cut(s) 177, 252
Hsp92II CATG 9 cut(s) 856, 872, 934, 1060, 1623, 1772, 1938, 1987, 2032
HspAI GCGC 9 cut(s) 5, 38, 107, 145, 153, 190, 480, 482, 1144
KflI GGGWCCC 1 cut(s) 719
Kpn2I TCCGGA 1 cut(s) 1399
KroI GCCGGC 1 cut(s) 582
KroNI GCCGGC 1 cut(s) 584
KspI CCGCGG 1 cut(s) 1046
Kzo9I GATC 4 cut(s) 769, 1312, 1533, 1591
LmnI GCTCC 5 cut(s) 78, 543, 544, 1111, 2245
Lsp1109I GCAGC 2 cut(s) 2123, 2294
LweI GCATC 5 cut(s) 1490, 1861, 1961, 2053, 2185
MabI ACCWGGT 2 cut(s) 1206, 1757
MaeI CTAG 7 cut(s) 348, 1089, 1181, 1247, 1542, 2271, 2303
MaeII ACGT 4 cut(s) 177, 468, 1487, 2431
MaeIII GTNAC 3 cut(s) 1280, 1669, 1990
MalI GATC 4 cut(s) 771, 1314, 1535, 1593
MbiI CCGCTC 1 cut(s) 73
MboI GATC 4 cut(s) 769, 1312, 1533, 1591
MboII GAAGA 8 cut(s) 634, 999, 1139, 1247, 1377, 1487, 1545, 2395
MfeI CAATTG 1 cut(s) 1629
MflI RGATCY 2 cut(s) 1312, 1591
MhlI GDGCHC 4 cut(s) 29, 604, 1108, 2170
MluCI AATT 9 cut(s) 10, 134, 306, 950, 1332, 1629, 1712, 1829, 1893
MlyI GAGTC 4 cut(s) 194, 230, 2047, 2069
MmeI TCCRAC 4 cut(s) 273, 1998, 2172, 2319
MroI TCCGGA 1 cut(s) 1399
MroNI GCCGGC 1 cut(s) 582
MroXI GAANNNNTTC 1 cut(s) 635
MseI TTAA 6 cut(s) 534, 678, 792, 804, 1413, 2259
MslI CAYNNNNRTG 5 cut(s) 575, 1079, 1358, 1944, 1988
MspA1I CMGCKG 1 cut(s) 1045
MspCI CTTAAG 1 cut(s) 1412
MunI CAATTG 1 cut(s) 1629
MvaI CCWGG 5 cut(s) 739, 1208, 1759, 2040, 2247
MvnI CGCG 9 cut(s) 38, 107, 109, 153, 186, 372, 482, 1045, 1347
NaeI GCCGGC 1 cut(s) 584
NciI CCSGG 9 cut(s) 59, 312, 359, 542, 696, 711, 712, 1013, 1978
NdeII GATC 4 cut(s) 769, 1312, 1533, 1591
NgoMIV GCCGGC 1 cut(s) 582
NlaIII CATG 9 cut(s) 856, 872, 934, 1060, 1623, 1772, 1938, 1987, 2032
NmeAIII GCCGAG 2 cut(s) 154, 1018
NmuCI GTSAC 2 cut(s) 1280, 1990
NsbI TGCGCA 1 cut(s) 1145
NspI RCATGY 2 cut(s) 856, 1938
OliI CACNNNNGTG 1 cut(s) 1079
PaeR7I CTCGAG 1 cut(s) 394
PagI TCATGA 1 cut(s) 1768
PalAI GGCGCGCC 1 cut(s) 480
PauI GCGCGC 1 cut(s) 480
PceI AGGCCT 3 cut(s) 1229, 1727, 1844
PcsI WCGNNNNNNNCGW 7 cut(s) 126, 174, 183, 231, 359, 392, 698
PdiI GCCGGC 1 cut(s) 584
PdmI GAANNNNTTC 1 cut(s) 635
Pfl23II CGTACG 1 cut(s) 465
PflFI GACNNNGTC 2 cut(s) 176, 1217
PfoI TCCNGGA 1 cut(s) 540
PinAI ACCGGT 1 cut(s) 43
PleI GAGTC 4 cut(s) 194, 230, 2047, 2069
PpsI GAGTC 4 cut(s) 194, 230, 2047, 2069
PpuMI RGGWCCY 1 cut(s) 719
Psp5II RGGWCCY 1 cut(s) 719
Psp6I CCWGG 5 cut(s) 737, 1206, 1757, 2038, 2245
PspFI CCCAGC 1 cut(s) 156
PspGI CCWGG 5 cut(s) 737, 1206, 1757, 2038, 2245
PspLI CGTACG 1 cut(s) 465
PspOMI GGGCCC 1 cut(s) 600
PspPPI RGGWCCY 1 cut(s) 719
PstNI CAGNNNCTG 1 cut(s) 1994
PsuI RGATCY 2 cut(s) 1312, 1591
PsyI GACNNNGTC 2 cut(s) 176, 1217
PteI GCGCGC 1 cut(s) 480
RigI GGCCGGCC 1 cut(s) 586
RsaI GTAC 7 cut(s) 329, 467, 500, 922, 1903, 1933, 2448
RsaNI GTAC 7 cut(s) 328, 466, 499, 921, 1902, 1932, 2447
RseI CAYNNNNRTG 5 cut(s) 575, 1079, 1358, 1944, 1988
SacII CCGCGG 1 cut(s) 1046
SalI GTCGAC 1 cut(s) 926
SaqAI TTAA 6 cut(s) 534, 678, 792, 804, 1413, 2259
Sau3AI GATC 4 cut(s) 769, 1312, 1533, 1591
ScaI AGTACT 1 cut(s) 329
SchI GAGTC 4 cut(s) 194, 230, 2047, 2069
SduI GDGCHC 4 cut(s) 29, 604, 1108, 2170
SexAI ACCWGGT 2 cut(s) 1206, 1757
SfaNI GCATC 5 cut(s) 1490, 1861, 1961, 2053, 2185
SfcI CTRYAG 2 cut(s) 1729, 1846
Sfr274I CTCGAG 1 cut(s) 394
Sfr303I CCGCGG 1 cut(s) 1046
SgrAI CRCCGGYG 1 cut(s) 43
SgrBI CCGCGG 1 cut(s) 1046
SgsI GGCGCGCC 1 cut(s) 480
SinI GGWCC 5 cut(s) 97, 698, 719, 1974, 1980
SlaI CTCGAG 1 cut(s) 394
SmaI CCCGGG 1 cut(s) 712
SmiMI CAYNNNNRTG 5 cut(s) 575, 1079, 1358, 1944, 1988
SmlI CTYRAG 3 cut(s) 295, 394, 1412
SmoI CTYRAG 3 cut(s) 295, 394, 1412
SpeI ACTAGT 2 cut(s) 1246, 1541
Sse9I AATT 9 cut(s) 10, 134, 306, 950, 1332, 1629, 1712, 1829, 1893
SseBI AGGCCT 3 cut(s) 1229, 1727, 1844
SspI AATATT 1 cut(s) 865
SspMI CTAG 7 cut(s) 348, 1089, 1181, 1247, 1542, 2271, 2303
StuI AGGCCT 3 cut(s) 1229, 1727, 1844
StyI CCWWGG 2 cut(s) 1464, 2270
TaaI ACNGT 9 cut(s) 223, 925, 1123, 1289, 1639, 1648, 1669, 1996, 2359
TaiI ACGT 4 cut(s) 180, 471, 1490, 2434
TaqI TCGA 9 cut(s) 168, 198, 395, 423, 474, 639, 927, 1484, 1536
TaqII GACCGA 1 cut(s) 782
TasI AATT 9 cut(s) 10, 134, 306, 950, 1332, 1629, 1712, 1829, 1893
TatI WGTACW 5 cut(s) 327, 498, 920, 1901, 1931
Tru1I TTAA 6 cut(s) 534, 678, 792, 804, 1413, 2259
Tru9I TTAA 6 cut(s) 534, 678, 792, 804, 1413, 2259
TscAI CASTG 5 cut(s) 1651, 1974, 1995, 2259, 2338
TseFI GTSAC 2 cut(s) 1280, 1990
TseI GCWGC 2 cut(s) 2111, 2282
Tsp45I GTSAC 2 cut(s) 1280, 1990
TspDTI ATGAA 9 cut(s) 857, 1286, 1677, 1719, 1736, 1836, 1853, 1962, 2487
TspGWI ACGGA 3 cut(s) 107, 433, 690
TspMI CCCGGG 1 cut(s) 710
TspRI CASTG 5 cut(s) 1651, 1974, 1995, 2259, 2338
Tth111I GACNNNGTC 2 cut(s) 176, 1217
Vha464I CTTAAG 1 cut(s) 1412
VneI GTGCAC 1 cut(s) 2166
VpaK11BI GGWCC 5 cut(s) 97, 698, 719, 1974, 1980
XapI RAATTY 2 cut(s) 10, 134
XceI RCATGY 2 cut(s) 856, 1938
XhoI CTCGAG 1 cut(s) 394
XmaI CCCGGG 1 cut(s) 710
XmaJI CCTAGG 1 cut(s) 2270
XmiI GTMKAC 3 cut(s) 927, 1197, 1212
XmnI GAANNNNTTC 1 cut(s) 635
XspI CTAG 7 cut(s) 348, 1089, 1181, 1247, 1542, 2271, 2303
ZraI GACGTC 1 cut(s) 178
ZrmI AGTACT 1 cut(s) 329
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.