Rw2G005740

CRS2-associated factor 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0013996
Physical Location & Seq
Forward (+)
5480091 .. 5483406
3316 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G005740.1

Sequence Viewer

Length: 2484 bp
ATGAAAGTAGAAGCACGAAATGGCGCTAAATTTGCCGACTTCGTGCCCCATTTTCGCGCCACCGGTGAACCCGAACCGGCCCACAACCCGCTCCAGACCCGCCCGCCCACGGAGGTCCGATTCGCGAGGTGGAACAACGCCAACGCCGAGAAATTCAACCAGCGCCGCCGCGCCCAGCAAGAAATCGAGGACGACGTCCGCCGCAAGCGCCGTTTCGAATCCGCCACCAGAATCGCCACCGTCGCCGCCGACGAATCCTCCTCCTCCGACGCCGAACCAACATTCAAATCCATAGGCACGCCTTCGTCTCCCTCAAGCCCTTCAATTCCCGGCAGAAAATCCAAGTACTCCAAGAACCCTAACCCTAGCTCGCACCCGGCGTTTCGCCGCGTCATCAGACCCACGAAGCTCTCGAGTATCGCCAGAGAGAAGCCGGAAATCGACCGGAAAGCTAACATCTCCGTCGGCGACGACGGGCTTTCGTACGTCATCGACGGCGCGCCGTTTGAGTTCAAGTACAGCTACACCGAGACCCCGAAGCAGAAGCCCATTAAGCTCCGGGAGCCGCCCTACGCGCCGTTCGGCCCCACCACGATGGGCCGGCCGTGGACGGGTCGGGCCCCATTGCCTCCGAGCAAGAAGAAACTGAAGGAGTTCGATTCGTTCCAGCTACCGCCGCCGCATAAGAAAGGGGTTAAGCCGGTGCAGTCGCCGGGTCCGTATTTACCCGGGTCGGGTCCCAAGTATGTCAAGTCCAGGGAGGAGATATTGGGTGAGCCGTTGACCGATCAGGAAGTCAAAGACTTGGTTAATAGCTGTGTTAAGACCAGAAGGCAGCTCAATATGGGTAGAGATGGATTGACACACAACATGCTGGATAATATTCATGCTCATTGGAAGCGGCGGAGAGTGTGCAAGATAAAATGTAAAGGGGTGTGTACAGTCGACATGGAAAATGTGTGCCAGCAATTAGAGGAAAGAACTGGAGGGAAAATCATTTATAGAAGAGGTGGGGTGATTTTTCTTTTCCGGGGGAGAAACTACAACTATAAAACTCGGCCGCGGTTTCCTCTCATGCTTTGGAGACCTATCACTCCTGTGTATCCTAGATTGATTCAGAGGGCTCCTGAGGGACTAACAGTTGAAGAAGCAACTGAAATGCGCAAGAAGGGGAGGAATCTGATACCAATACGCAAGCTAGGAAAAAATGGAGTATACTCTGACCTGGTAGACAATGTCAGAGAGGCCTTTGAAGAGTGTGAACTAGTGCGAATAGATTGTCAAGGGATGAATGGGAGTGACTACCGTAAAATAGGTGCTAAACTAAAGGATCTTGTCCCTTGTGTGCTAATTTCCTTTGAACGCGAGAACATTCTTGTGTGGAGAGGACGGGAATGGAAGTCTTCTTTCCTACATCCAGAGAGTAATCTTAAGGAAGTCAAAGAATCTGATGTTGATGATTCACCTTCTATTGCACCTTCCTTGGAAGGTGAAGAAGCATCGACGTTATGTGCTTTTACAGCTTCAGTCAATGACGAAAATCCTCAAATGTTCGACACTAGTATCACCTCTTCAAGTTCTGAAGTTGTGGGTGCTGAGGGAAATGGGGATCCATCCCCTTCACCATATATTGAGCCATGTGCCACAATTGATACAGTTTCCACTGTGGGAGGCACTTATGAAACTGTAACCATCTCAGACATCAAAGGCTTTAGAAATGATGAAGCAGAATTGGAGAGGAAGGCCTATAGCAGTTCAACCATACCACAGGATACCAGGTATGCTCATGATGAGGCAGAAACGATTTCCATCTCAGACATCAAGGGCTTTAGAAATGATGAAGCAGAATTGGAAATGAAGGCCTATAGCAGTTCAACCTTACCGCAGGATGCTGATGATGAGGCCGAAACAATTTCCAGTACATCTGGAACTGAAAACATAGTGGATAGTACATGCCATACTGATGAAGCATCAGCAACTACATCAGTGGGGACCGGGACCATGCCAGTGACTGTTGAAAGCACTGAAACGAAGTTGGATTCCCTCATGGTTGCCCCTGGTAACTCGCAAAATCTCAATGAGCAGGCAAGGTTGAGTGCAGCCTGTAAAGAGAAGGTGCTATCACTCTTGAACGAAGCTGTTGGGAGTGGGAGTGCACTTATTTTAGACGATTCGTCGTTGGATGCTGACATCATATATCAGAGGGCAGTTGCTCTCGCCAAGTCTGCTCCACCTGGGCCAGTGTTTAAGCATCGACCTAGGAGAGTTGCAGCTCAAATGAGTAAGAAGCTAGTAGTAAGAAAACAGAAGCAAGAAGCCACTGAGTTGGAAGTGAAAGAGATAACAGTTTATGCGAAGAAGGGAAGTGAGAAGAAGGATTCCAAAGTTCACAGAACAAAAACAAGAGATTTTGGTGAACCTCTTGATAGCATTGTACCACAGGGAAGCCTGAGAGTTGATGAACTTGCTAAGCTATTAGCCTGA

Protein Analysis

827

Amino Acids

91.89

Weight (kDa)

8.63

Isoelectric Point (pI)

49.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRS1_YhbY PF01985 261 - 344 1.1e-20 CRS1 / YhbY (CRM) domain
CRS1_YhbY PF01985 379 - 462 1.4e-16 CRS1 / YhbY (CRM) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013996)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G20020
fragaria_vesca FvH4_1g05780
malus_domestica MD02G1059500.v1.1 MD15G1193500.v1.1
prunus_persica Prupe.7G223600_v2.0.a1
pyrus_communis pycom02g04850 pycom15g17320
rosa_chinensis RchiOBHm_Chr2g0091431
rosa_laevigata RLG00000016223
rosa_multiflora Rmu_ssc0000333.1_g000008
rosa_roxburghii Rroxscaffold_2G00150090
rosa_rugosa Rorug02G0017800
rosa_samantha Rh2AG062700 Rh2BG062000 Rh2CG063900 Rh2DG062300
rosa_wichuraiana Rw2G005740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 198
Acc16I TGCGCA 1 cut(s) 1163
AccBSI CCGCTC 1 cut(s) 91
AccI GTMKAC 3 cut(s) 945, 1215, 1230
AccII CGCG 8 cut(s) 57, 125, 171, 390, 500, 575, 1063, 1365
AclWI GGATC 3 cut(s) 1338, 1604, 1617
AcoI YGGCCR 2 cut(s) 602, 1058
AcsI RAATTY 2 cut(s) 29, 152
AcuI CTGAAG 3 cut(s) 668, 1509, 1602
AcyI GRCGYC 2 cut(s) 195, 270
AfaI GTAC 7 cut(s) 347, 485, 518, 940, 1921, 1951, 2436
AfiI CCNNNNNNNGG 3 cut(s) 109, 611, 734
AflII CTTAAG 1 cut(s) 1430
AgeI ACCGGT 1 cut(s) 62
AhdI GACNNNNNGTC 1 cut(s) 2173
AhlI ACTAGT 2 cut(s) 1264, 1559
AjnI CCWGG 5 cut(s) 755, 1224, 1775, 2056, 2233
AleI CACNNNNGTG 1 cut(s) 1097
Alw21I GWGCWC 1 cut(s) 2158
Alw26I GTCTC 3 cut(s) 312, 524, 1078
Alw44I GTGCAC 1 cut(s) 2154
AlwI GGATC 3 cut(s) 1338, 1604, 1617
AlwNI CAGNNNCTG 1 cut(s) 2012
Ama87I CYCGRG 2 cut(s) 412, 728
ApaI GGGCCC 1 cut(s) 622
ApaLI GTGCAC 1 cut(s) 2154
ApeKI GCWGC 3 cut(s) 835, 2099, 2270
ApoI RAATTY 2 cut(s) 29, 152
ArsI GACNNNNNNTTYG 2 cut(s) 1264, 1296
AscI GGCGCGCC 1 cut(s) 498
AsiGI ACCGGT 1 cut(s) 62
Asp700I GAANNNNTTC 1 cut(s) 653
AspA2I CCTAGG 1 cut(s) 2258
AspLEI GCGC 9 cut(s) 26, 59, 165, 173, 210, 500, 502, 577, 1164
AsuC2I CCSGG 8 cut(s) 330, 377, 560, 714, 729, 730, 1031, 1996
AsuHPI GGTGA 8 cut(s) 77, 785, 1027, 1455, 1502, 1558, 1614, 2426
AsuII TTCGAA 1 cut(s) 216
AvaI CYCGRG 2 cut(s) 412, 728
AvaII GGWCC 5 cut(s) 115, 716, 737, 1992, 1998
AvrII CCTAGG 1 cut(s) 2258
AxyI CCTNAGG 1 cut(s) 1128
BaeGI GKGCMC 3 cut(s) 48, 622, 2158
BaeI ACNNNNGTAYC 2 cut(s) 1546, 1579
BamHI GGATCC 1 cut(s) 1609
BanII GRGCYC 2 cut(s) 622, 1126
BarI GAAGNNNNNNTAC 2 cut(s) 1555, 1587
BbsI GAAGAC 1 cut(s) 1395
Bbv12I GWGCWC 1 cut(s) 2158
BbvCI CCTCAGC 1 cut(s) 1596
BbvI GCAGC 3 cut(s) 847, 2111, 2282
BccI CCATC 5 cut(s) 589, 848, 1621, 1700, 1817
BceAI ACGGC 6 cut(s) 195, 487, 511, 562, 589, 763
BciT130I CCWGG 5 cut(s) 757, 1226, 1777, 2058, 2235
BciVI GTATCC 2 cut(s) 1113, 1765
BcnI CCSGG 8 cut(s) 330, 377, 560, 714, 729, 730, 1031, 1996
BcoDI GTCTC 3 cut(s) 312, 524, 1078
BcuI ACTAGT 2 cut(s) 1264, 1559
BfaI CTAG 7 cut(s) 366, 1107, 1199, 1265, 1560, 2259, 2291
BfmI CTRYAG 2 cut(s) 1747, 1864
BfoI RGCGCY 3 cut(s) 27, 166, 211
BfrI CTTAAG 1 cut(s) 1430
BfuI GTATCC 2 cut(s) 1113, 1765
BlnI CCTAGG 1 cut(s) 2258
BlpI GCTNAGC 1 cut(s) 2469
BmcAI AGTACT 1 cut(s) 347
Bme18I GGWCC 5 cut(s) 115, 716, 737, 1992, 1998
BmeRI GACNNNNNGTC 1 cut(s) 2173
BmeT110I CYCGRG 2 cut(s) 412, 728
BmsI GCATC 5 cut(s) 1508, 1879, 1979, 2173, 2260
BpiI GAAGAC 1 cut(s) 1395
BpmI CTGGAG 2 cut(s) 77, 1005
Bpu10I CCTNAGC 1 cut(s) 1596
Bpu1102I GCTNAGC 1 cut(s) 2469
Bpu14I TTCGAA 1 cut(s) 216
BpuEI CTTGAG 1 cut(s) 298
BpuMI CCSGG 8 cut(s) 330, 377, 560, 714, 729, 730, 1031, 1996
BsaBI GATNNNNATC 1 cut(s) 1808
BsaHI GRCGYC 2 cut(s) 195, 270
BsaI GGTCTC 2 cut(s) 524, 1078
BsaWI WCCGGW 2 cut(s) 62, 444
BsaXI ACNNNNNCTCC 4 cut(s) 242, 272, 2137, 2167
Bsc4I CCNNNNNNNGG 3 cut(s) 109, 611, 734
Bse118I RCCGGY 4 cut(s) 62, 76, 600, 700
Bse1I ACTGG 4 cut(s) 988, 1917, 2006, 2240
Bse21I CCTNAGG 1 cut(s) 1128
Bse3DI GCAATG 1 cut(s) 623
Bse8I GATNNNNATC 1 cut(s) 1808
BseBI CCWGG 5 cut(s) 757, 1226, 1777, 2058, 2235
BseGI GGATG 5 cut(s) 1293, 1414, 1613, 1894, 2188
BseJI GATNNNNATC 1 cut(s) 1808
BseLI CCNNNNNNNGG 3 cut(s) 109, 611, 734
BseMI GCAATG 1 cut(s) 623
BseMII CTCAG 6 cut(s) 1119, 1587, 1710, 1827, 2313, 2441
BseNI ACTGG 4 cut(s) 988, 1917, 2006, 2240
BsePI GCGCGC 1 cut(s) 498
BseRI GAGGAG 3 cut(s) 250, 253, 776
BseSI GKGCMC 3 cut(s) 48, 622, 2158
BseX3I CGGCCG 2 cut(s) 602, 1058
BseXI GCAGC 3 cut(s) 847, 2111, 2282
BseYI CCCAGC 1 cut(s) 174
BsgI GTGCAG 2 cut(s) 725, 2118
Bsh1236I CGCG 8 cut(s) 57, 125, 171, 390, 500, 575, 1063, 1365
Bsh1285I CGRYCG 3 cut(s) 445, 605, 1061
BshTI ACCGGT 1 cut(s) 62
BsiEI CGRYCG 3 cut(s) 445, 605, 1061
BsiHKAI GWGCWC 1 cut(s) 2158
BsiHKCI CYCGRG 2 cut(s) 412, 728
BsiWI CGTACG 1 cut(s) 483
BslFI GGGAC 5 cut(s) 723, 1146, 1322, 2005, 2011
BslI CCNNNNNNNGG 3 cut(s) 109, 611, 734
BsmAI GTCTC 3 cut(s) 312, 524, 1078
BsmBI CGTCTC 1 cut(s) 312
BsmFI GGGAC 5 cut(s) 723, 1146, 1322, 2005, 2011
Bso31I GGTCTC 2 cut(s) 524, 1078
BsoBI CYCGRG 2 cut(s) 412, 728
Bsp119I TTCGAA 1 cut(s) 216
Bsp120I GGGCCC 1 cut(s) 618
Bsp1286I GDGCHC 4 cut(s) 48, 622, 1126, 2158
Bsp1407I TGTACA 1 cut(s) 938
Bsp143I GATC 3 cut(s) 787, 1330, 1609
Bsp1720I GCTNAGC 1 cut(s) 2469
Bsp68I TCGCGA 1 cut(s) 125
BspCNI CTCAG 6 cut(s) 1120, 1588, 1709, 1826, 2314, 2442
BspFNI CGCG 8 cut(s) 57, 125, 171, 390, 500, 575, 1063, 1365
BspHI TCATGA 1 cut(s) 1786
BspPI GGATC 3 cut(s) 1338, 1604, 1617
BspT104I TTCGAA 1 cut(s) 216
BspTI CTTAAG 1 cut(s) 1430
BspTNI GGTCTC 2 cut(s) 524, 1078
BsrBI CCGCTC 1 cut(s) 91
BsrDI GCAATG 1 cut(s) 623
BsrFI RCCGGY 4 cut(s) 62, 76, 600, 700
BsrGI TGTACA 1 cut(s) 938
BsrI ACTGG 4 cut(s) 988, 1917, 2006, 2240
BssAI RCCGGY 4 cut(s) 62, 76, 600, 700
BssHII GCGCGC 1 cut(s) 498
BssMI GATC 3 cut(s) 787, 1330, 1609
BssNAI GTATAC 1 cut(s) 1216
BssNI GRCGYC 2 cut(s) 195, 270
BssT1I CCWWGG 2 cut(s) 1482, 2258
Bst1107I GTATAC 1 cut(s) 1216
Bst2UI CCWGG 5 cut(s) 757, 1226, 1777, 2058, 2235
Bst4CI ACNGT 9 cut(s) 241, 943, 1141, 1307, 1657, 1666, 1687, 2014, 2347
Bst6I CTCTTC 3 cut(s) 1000, 1248, 1576
BstACI GRCGYC 2 cut(s) 195, 270
BstAFI CTTAAG 1 cut(s) 1430
BstAUI TGTACA 1 cut(s) 938
BstBI TTCGAA 1 cut(s) 216
BstC8I GCNNGC 9 cut(s) 104, 206, 299, 371, 500, 602, 965, 1196, 2085
BstDEI CTNAG 7 cut(s) 1128, 1596, 1696, 1813, 2322, 2450, 2469
BstDSI CCRYGG 3 cut(s) 108, 605, 1061
BstF5I GGATG 5 cut(s) 1293, 1414, 1613, 1894, 2188
BstFNI CGCG 8 cut(s) 57, 125, 171, 390, 500, 575, 1063, 1365
BstH2I RGCGCY 3 cut(s) 27, 166, 211
BstHHI GCGC 9 cut(s) 26, 59, 165, 173, 210, 500, 502, 577, 1164
BstKTI GATC 3 cut(s) 790, 1333, 1612
BstMAI GTCTC 3 cut(s) 312, 524, 1078
BstMBI GATC 3 cut(s) 787, 1330, 1609
BstMCI CGRYCG 3 cut(s) 445, 605, 1061
BstMWI GCNNNNNNNGC 9 cut(s) 32, 207, 242, 553, 562, 574, 676, 1520, 2225
BstNI CCWGG 5 cut(s) 757, 1226, 1777, 2058, 2235
BstNSI RCATGY 2 cut(s) 874, 1956
BstSFI CTRYAG 2 cut(s) 1747, 1864
BstSLI GKGCMC 3 cut(s) 48, 622, 2158
BstUI CGCG 8 cut(s) 57, 125, 171, 390, 500, 575, 1063, 1365
BstV1I GCAGC 3 cut(s) 847, 2111, 2282
BstV2I GAAGAC 1 cut(s) 1395
BstX2I RGATCY 2 cut(s) 1330, 1609
BstXI CCANNNNNNTGG 2 cut(s) 595, 2326
BstYI RGATCY 2 cut(s) 1330, 1609
BstZ17I GTATAC 1 cut(s) 1216
BstZI CGGCCG 2 cut(s) 602, 1058
Bsu36I CCTNAGG 1 cut(s) 1128
BsuI GTATCC 2 cut(s) 1113, 1765
BtgI CCRYGG 3 cut(s) 108, 605, 1061
BtsCI GGATG 5 cut(s) 1293, 1414, 1613, 1894, 2188
BtsIMutI CAGTG 6 cut(s) 1662, 1992, 2013, 2022, 2247, 2319
BtuMI TCGCGA 1 cut(s) 125
Cac8I GCNNGC 9 cut(s) 104, 206, 299, 371, 500, 602, 965, 1196, 2085
CaiI CAGNNNCTG 1 cut(s) 2012
CciI TCATGA 1 cut(s) 1786
CfoI GCGC 9 cut(s) 26, 59, 165, 173, 210, 500, 502, 577, 1164
Cfr10I RCCGGY 4 cut(s) 62, 76, 600, 700
Cfr42I CCGCGG 1 cut(s) 1064
Cfr9I CCCGGG 1 cut(s) 728
CseI GACGC 2 cut(s) 278, 379
CsiI ACCWGGT 2 cut(s) 1224, 1775
Csp6I GTAC 7 cut(s) 346, 484, 517, 939, 1920, 1950, 2435
CspAI ACCGGT 1 cut(s) 62
CviAII CATG 9 cut(s) 871, 887, 949, 1075, 1638, 1787, 1953, 2002, 2047
CviQI GTAC 7 cut(s) 346, 484, 517, 939, 1920, 1950, 2435
DdeI CTNAG 7 cut(s) 1128, 1596, 1696, 1813, 2322, 2450, 2469
DpnI GATC 3 cut(s) 789, 1332, 1611
DpnII GATC 3 cut(s) 787, 1330, 1609
DriI GACNNNNNGTC 1 cut(s) 2173
EaeI YGGCCR 2 cut(s) 602, 1058
EagI CGGCCG 2 cut(s) 602, 1058
Eam1104I CTCTTC 3 cut(s) 1000, 1248, 1576
Eam1105I GACNNNNNGTC 1 cut(s) 2173
EarI CTCTTC 3 cut(s) 1000, 1248, 1576
EciI GGCGGA 3 cut(s) 188, 211, 919
EclXI CGGCCG 2 cut(s) 602, 1058
Eco130I CCWWGG 2 cut(s) 1482, 2258
Eco147I AGGCCT 3 cut(s) 1247, 1745, 1862
Eco24I GRGCYC 2 cut(s) 622, 1126
Eco31I GGTCTC 2 cut(s) 524, 1078
Eco47I GGWCC 5 cut(s) 115, 716, 737, 1992, 1998
Eco52I CGGCCG 2 cut(s) 602, 1058
Eco57I CTGAAG 3 cut(s) 668, 1509, 1602
Eco81I CCTNAGG 1 cut(s) 1128
Eco88I CYCGRG 2 cut(s) 412, 728
EcoO109I RGGNCCY 2 cut(s) 619, 737
EcoRII CCWGG 5 cut(s) 755, 1224, 1775, 2056, 2233
EcoT14I CCWWGG 2 cut(s) 1482, 2258
EcoT38I GRGCYC 2 cut(s) 622, 1126
ErhI CCWWGG 2 cut(s) 1482, 2258
Esp3I CGTCTC 1 cut(s) 312
FaeI CATG 9 cut(s) 874, 890, 952, 1078, 1641, 1790, 1956, 2005, 2050
FaqI GGGAC 5 cut(s) 723, 1146, 1322, 2005, 2011
FatI CATG 9 cut(s) 870, 886, 948, 1074, 1637, 1786, 1952, 2001, 2046
FauI CCCGC 3 cut(s) 96, 107, 111
FblI GTMKAC 3 cut(s) 945, 1215, 1230
FokI GGATG 5 cut(s) 1300, 1401, 1600, 1901, 2195
FriOI GRGCYC 2 cut(s) 622, 1126
FseI GGCCGGCC 1 cut(s) 604
FspBI CTAG 7 cut(s) 366, 1107, 1199, 1265, 1560, 2259, 2291
FspI TGCGCA 1 cut(s) 1163
GlaI GCGC 9 cut(s) 25, 58, 164, 172, 209, 499, 501, 576, 1163
GsaI CCCAGC 1 cut(s) 178
GsuI CTGGAG 2 cut(s) 77, 1005
HaeII RGCGCY 3 cut(s) 27, 166, 211
HgaI GACGC 2 cut(s) 278, 379
HhaI GCGC 9 cut(s) 26, 59, 165, 173, 210, 500, 502, 577, 1164
Hin1I GRCGYC 2 cut(s) 195, 270
Hin1II CATG 9 cut(s) 874, 890, 952, 1078, 1641, 1790, 1956, 2005, 2050
Hin6I GCGC 9 cut(s) 24, 57, 163, 171, 208, 498, 500, 575, 1162
HinP1I GCGC 9 cut(s) 24, 57, 163, 171, 208, 498, 500, 575, 1162
HincII GTYRAC 2 cut(s) 783, 946
HindII GTYRAC 2 cut(s) 783, 946
HphI GGTGA 8 cut(s) 77, 785, 1027, 1455, 1502, 1558, 1614, 2426
HpyCH4III ACNGT 9 cut(s) 241, 943, 1141, 1307, 1657, 1666, 1687, 2014, 2347
HpyCH4IV ACGT 3 cut(s) 195, 486, 1505
HpyCH4V TGCA 6 cut(s) 706, 915, 1475, 2099, 2156, 2270
HpyF10VI GCNNNNNNNGC 9 cut(s) 32, 207, 242, 553, 562, 574, 676, 1520, 2225
HpyF3I CTNAG 7 cut(s) 1128, 1596, 1696, 1813, 2322, 2450, 2469
HpySE526I ACGT 3 cut(s) 195, 486, 1505
Hsp92I GRCGYC 2 cut(s) 195, 270
Hsp92II CATG 9 cut(s) 874, 890, 952, 1078, 1641, 1790, 1956, 2005, 2050
HspAI GCGC 9 cut(s) 24, 57, 163, 171, 208, 498, 500, 575, 1162
KflI GGGWCCC 1 cut(s) 737
KroI GCCGGC 1 cut(s) 600
KroNI GCCGGC 1 cut(s) 602
KspI CCGCGG 1 cut(s) 1064
Kzo9I GATC 3 cut(s) 787, 1330, 1609
LmnI GCTCC 5 cut(s) 96, 561, 562, 1129, 2233
Lsp1109I GCAGC 3 cut(s) 847, 2111, 2282
LweI GCATC 5 cut(s) 1508, 1879, 1979, 2173, 2260
MabI ACCWGGT 2 cut(s) 1224, 1775
MaeI CTAG 7 cut(s) 366, 1107, 1199, 1265, 1560, 2259, 2291
MaeII ACGT 3 cut(s) 195, 486, 1505
MaeIII GTNAC 4 cut(s) 1298, 1687, 2008, 2060
MalI GATC 3 cut(s) 789, 1332, 1611
MbiI CCGCTC 1 cut(s) 91
MboI GATC 3 cut(s) 787, 1330, 1609
MboII GAAGA 9 cut(s) 652, 1017, 1157, 1265, 1395, 1505, 1563, 2368, 2383
MfeI CAATTG 1 cut(s) 1647
MflI RGATCY 2 cut(s) 1330, 1609
MhlI GDGCHC 4 cut(s) 48, 622, 1126, 2158
MluCI AATT 9 cut(s) 29, 152, 324, 968, 1350, 1647, 1730, 1847, 1911
MmeI TCCRAC 4 cut(s) 291, 2016, 2160, 2307
MroNI GCCGGC 1 cut(s) 600
MroXI GAANNNNTTC 1 cut(s) 653
MseI TTAA 6 cut(s) 552, 696, 810, 822, 1431, 2247
MslI CAYNNNNRTG 5 cut(s) 593, 1097, 1376, 1962, 2006
MspA1I CMGCKG 1 cut(s) 1063
MspCI CTTAAG 1 cut(s) 1430
MunI CAATTG 1 cut(s) 1647
MvaI CCWGG 5 cut(s) 757, 1226, 1777, 2058, 2235
MvnI CGCG 8 cut(s) 57, 125, 171, 390, 500, 575, 1063, 1365
MwoI GCNNNNNNNGC 9 cut(s) 32, 207, 242, 553, 562, 574, 676, 1520, 2225
NaeI GCCGGC 1 cut(s) 602
NciI CCSGG 8 cut(s) 330, 377, 560, 714, 729, 730, 1031, 1996
NdeII GATC 3 cut(s) 787, 1330, 1609
NgoMIV GCCGGC 1 cut(s) 600
NlaIII CATG 9 cut(s) 874, 890, 952, 1078, 1641, 1790, 1956, 2005, 2050
NmeAIII GCCGAG 2 cut(s) 172, 1036
NmuCI GTSAC 2 cut(s) 1298, 2008
NruI TCGCGA 1 cut(s) 125
NsbI TGCGCA 1 cut(s) 1163
NspI RCATGY 2 cut(s) 874, 1956
NspV TTCGAA 1 cut(s) 216
OliI CACNNNNGTG 1 cut(s) 1097
PaeR7I CTCGAG 1 cut(s) 412
PagI TCATGA 1 cut(s) 1786
PalAI GGCGCGCC 1 cut(s) 498
PauI GCGCGC 1 cut(s) 498
PceI AGGCCT 3 cut(s) 1247, 1745, 1862
PcsI WCGNNNNNNNCGW 6 cut(s) 144, 192, 249, 377, 410, 716
PdiI GCCGGC 1 cut(s) 602
PdmI GAANNNNTTC 1 cut(s) 653
Pfl23II CGTACG 1 cut(s) 483
PflFI GACNNNGTC 2 cut(s) 194, 1235
PfoI TCCNGGA 1 cut(s) 558
PinAI ACCGGT 1 cut(s) 62
PpuMI RGGWCCY 1 cut(s) 737
Psp5II RGGWCCY 1 cut(s) 737
Psp6I CCWGG 5 cut(s) 755, 1224, 1775, 2056, 2233
PspFI CCCAGC 1 cut(s) 174
PspGI CCWGG 5 cut(s) 755, 1224, 1775, 2056, 2233
PspLI CGTACG 1 cut(s) 483
PspOMI GGGCCC 1 cut(s) 618
PspPPI RGGWCCY 1 cut(s) 737
PstNI CAGNNNCTG 1 cut(s) 2012
PsuI RGATCY 2 cut(s) 1330, 1609
PsyI GACNNNGTC 2 cut(s) 194, 1235
PteI GCGCGC 1 cut(s) 498
RigI GGCCGGCC 1 cut(s) 604
RruI TCGCGA 1 cut(s) 125
RsaI GTAC 7 cut(s) 347, 485, 518, 940, 1921, 1951, 2436
RsaNI GTAC 7 cut(s) 346, 484, 517, 939, 1920, 1950, 2435
RseI CAYNNNNRTG 5 cut(s) 593, 1097, 1376, 1962, 2006
SacII CCGCGG 1 cut(s) 1064
SalI GTCGAC 1 cut(s) 944
SaqAI TTAA 6 cut(s) 552, 696, 810, 822, 1431, 2247
Sau3AI GATC 3 cut(s) 787, 1330, 1609
ScaI AGTACT 1 cut(s) 347
SduI GDGCHC 4 cut(s) 48, 622, 1126, 2158
SexAI ACCWGGT 2 cut(s) 1224, 1775
SfaNI GCATC 5 cut(s) 1508, 1879, 1979, 2173, 2260
SfcI CTRYAG 2 cut(s) 1747, 1864
Sfr274I CTCGAG 1 cut(s) 412
Sfr303I CCGCGG 1 cut(s) 1064
SfuI TTCGAA 1 cut(s) 216
SgrAI CRCCGGYG 1 cut(s) 62
SgrBI CCGCGG 1 cut(s) 1064
SgsI GGCGCGCC 1 cut(s) 498
SinI GGWCC 5 cut(s) 115, 716, 737, 1992, 1998
SlaI CTCGAG 1 cut(s) 412
SmaI CCCGGG 1 cut(s) 730
SmiMI CAYNNNNRTG 5 cut(s) 593, 1097, 1376, 1962, 2006
SmlI CTYRAG 3 cut(s) 313, 412, 1430
SmoI CTYRAG 3 cut(s) 313, 412, 1430
SpeI ACTAGT 2 cut(s) 1264, 1559
Sse9I AATT 9 cut(s) 29, 152, 324, 968, 1350, 1647, 1730, 1847, 1911
SseBI AGGCCT 3 cut(s) 1247, 1745, 1862
SspI AATATT 1 cut(s) 883
SspMI CTAG 7 cut(s) 366, 1107, 1199, 1265, 1560, 2259, 2291
StuI AGGCCT 3 cut(s) 1247, 1745, 1862
StyI CCWWGG 2 cut(s) 1482, 2258
TaaI ACNGT 9 cut(s) 241, 943, 1141, 1307, 1657, 1666, 1687, 2014, 2347
TaiI ACGT 3 cut(s) 198, 489, 1508
TaqII GACCGA 1 cut(s) 800
TasI AATT 9 cut(s) 29, 152, 324, 968, 1350, 1647, 1730, 1847, 1911
TatI WGTACW 5 cut(s) 345, 516, 938, 1919, 1949
Tru1I TTAA 6 cut(s) 552, 696, 810, 822, 1431, 2247
Tru9I TTAA 6 cut(s) 552, 696, 810, 822, 1431, 2247
TscAI CASTG 6 cut(s) 1669, 1992, 2013, 2029, 2247, 2326
TseFI GTSAC 2 cut(s) 1298, 2008
TseI GCWGC 3 cut(s) 835, 2099, 2270
Tsp45I GTSAC 2 cut(s) 1298, 2008
TspDTI ATGAA 9 cut(s) 17, 875, 1304, 1695, 1737, 1854, 1871, 1980, 2475
TspGWI ACGGA 3 cut(s) 125, 451, 708
TspMI CCCGGG 1 cut(s) 728
TspRI CASTG 6 cut(s) 1669, 1992, 2013, 2029, 2247, 2326
Tth111I GACNNNGTC 2 cut(s) 194, 1235
Vha464I CTTAAG 1 cut(s) 1430
VneI GTGCAC 1 cut(s) 2154
VpaK11BI GGWCC 5 cut(s) 115, 716, 737, 1992, 1998
XapI RAATTY 2 cut(s) 29, 152
XceI RCATGY 2 cut(s) 874, 1956
XhoI CTCGAG 1 cut(s) 412
XmaI CCCGGG 1 cut(s) 728
XmaJI CCTAGG 1 cut(s) 2258
XmiI GTMKAC 3 cut(s) 945, 1215, 1230
XmnI GAANNNNTTC 1 cut(s) 653
XspI CTAG 7 cut(s) 366, 1107, 1199, 1265, 1560, 2259, 2291
ZraI GACGTC 1 cut(s) 196
ZrmI AGTACT 1 cut(s) 347
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.