FvH4_1g05780

CRS2-associated factor 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0013996
Physical Location & Seq
Reverse (-)
3046315 .. 3049624
3310 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g05780.t1

Sequence Viewer

Length: 2415 bp
ATGGCGCTAAATTTGCCGACTTCGTGCCCCATTTTCGCTCCACCGGTGAACCCGAACCCGGCCCACAACCCGATACATACCCGCCCGCCCACGGAGGTCCGATTCGCGCGGTGGAACAACGCCAACGCCGAGAAATTCAACCAGCGCCGCCGCGCCCAGCAGGAAATCGAGGACGACTTCCGCCGCGAGCGCCGCTTCGACTCCGCCACCAGAATCGCCACCGTCTCCGTCGCCGGCTCCTCCACCTCCGACGCCGAGACCGCATTCAAGTCCATCGGCACGCCTTCGTCTCCGTCACGGCCTTCAATTCCGGGCAAGAAATCAAAGTACTCCGAAAACCCTAACCCTAACCCTAGCTCGCACCCGGCGTTTCGCCGAGTCATCAAGCCCACGAAGCTCTCGAGCATCACCAGAGAGAAGCCGGAAGTCGACCGGAAAGCCAACATCTCAATCGGCGACGACGGGCTGTCCTACGTCATCGACGGCGCGCCGTTTGAGTTCAAGTACAGCTACACCGAGACGCCGAAGCAGAAGCCGATTAAGCTCCGGGAGCCGCCGTACGCGCCGTTCGGCCCAACCACGATGGGCCGGCCGTGGACGGGTCGGGCCCCATTGCCGGCGAGCAAGAAGAAGATGAAGGAATTCGACTCGTTCCAGCTGCCGCCGCCGCACAAGAAAGGGGTGAGGCCGGTGCAGTCGCCGGGCCCGTATTTGCCCGGGTCGGGTCCTAAGTATGTCAAGTCCAGGGAGGAGATATTGGGCGACCCGTTGACCGATCAGGAAGTCAAAGACTTGGTTAATGGCTGCATCAAGACCAGGAGGCAACTCAATATGGGTAGAGATGGTTTGACACACAACATGCTGGATAATATTCATGCTCATTGGAAGCGGCGCAGAGTGTGCAAGATTAAATGTAAAGGAGTGTGTACAGTCGACATGGAAAATGTGTGCCAGCAATTAGAGGAAAGAACTGGAGGGAAAATTATTTATAGAAGAGGTGGTGTGATTTTTCTTTTCCGGGGGAGGAATTACAACTATAAGACTCGGCCGCGGTTTCCTCTCATGCTTTGGAGACCAATCACTCCTGTGTATCCTAGATTGATTCAGCGGGCTCCTGAGGGTCTAACGGTTGAAGAAGCAACTGAAATGCGCAAGAAGGGGAGGGATCTGATACCAATACGCAAGCTAGGAAAAAATGGAGTGTACTCTGACCTGGTAGACAATGTCAGAGAGGCCTTTGAAGAGTGTGAACTAGTGCGAATTGATTGTCAAGGGATGAATGGCAGTGACTACCGAAAAATAGGTGCTAAATTAAAGGATCTTGTCCCTTGTGTGCTAATTTCCTTTGAACGCGAGAGCATTCTTATGTGGAGAGGACGGGAATGGAAGTCATCTCTCGTAAATCCGGAGAGTAATCTTAAGGAAGTCAAAGAGTCTAATGTTGATGATTCACCTTCAATTGCACTTTCCTTGGAAGGTGAAGATGCATCGACAGTATGTGCTTTTACAGGTTCAGTCAAGGACGCAAATCCTGAAATGATCGACACTAGTATATCCTCTTCAATTGCTGAAGTTGTGGGTGCAGAGGGAACTGAGGATCCATCCCCTTCACCATATATTGAGCCACCTGCCATAATTGACACAGTTTCCGACGTGGGAAGCACTTGTGAAACTGTAACCATATCGGACATCAAAGGCTTTAGAGATGATGAAGCAGAACTGAATATGAAGGCCTATAGCAGTTTGGTCATACCAGAAGATACCAGCTACGCAGATGATGAGTCAGAAACAATTTCCAGTACATCTGGAACTGAAGACATATTGGATAATACACGCCATGCTGATGAAGCTTCACCAACTACATCAGTGGGGACTGGGGCCATTCTAGTAACTGTTGAAAACACTGAAACAAAGTTGAATACCCTCATGGAGTCCCCTGGTAGTAACAAGACTCCGCAAGATGCTTCTGTAGCTTCACAAAATCTCAATGAGCGAGCAAAGTTGTGTGCAGCGTGCAAAGAGAAAGTGTTGTCACTCTTGAATGAAGCTGTTGGGAGTGGGAGTGCACTTATTTTAGATGATTCGTCGTTGGATGCTGACATCATATATCAGAGAGCAGTTGATCTCGCCAAGTCTGCTCCGCCTGGGCCAGTGTTTAAGCATCGATCTAGTAGAGGTTCAGCTCAAATGAGGAAGAAGCTAGTAGTAAGAAAACAGAAGCAAGAAGCCACGGAGTTGGAAGTGAAAGAGATTACAGTTTATGATATGCAAAGAAATTTGGGCGAGAAGAAGAGAAGTGAGAGGAAGGATTCCAAAGTTCACAGAACAAGAACAAGAGATTTTGGTGAACCTCTAGATAGCATTGTACCACAAGGAAGCCTGAGAGTCGATGAACTTGCTAAGCTATTAGCATGA

Protein Analysis

805

Amino Acids

89.3

Weight (kDa)

8.7

Isoelectric Point (pI)

48.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRS1_YhbY PF01985 257 - 340 2.6e-20 CRS1 / YhbY (CRM) domain
CRS1_YhbY PF01985 375 - 458 1.2e-16 CRS1 / YhbY (CRM) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013996)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G20020
fragaria_vesca FvH4_1g05780
malus_domestica MD02G1059500.v1.1 MD15G1193500.v1.1
prunus_persica Prupe.7G223600_v2.0.a1
pyrus_communis pycom02g04850 pycom15g17320
rosa_chinensis RchiOBHm_Chr2g0091431
rosa_laevigata RLG00000016223
rosa_multiflora Rmu_ssc0000333.1_g000008
rosa_roxburghii Rroxscaffold_2G00150090
rosa_rugosa Rorug02G0017800
rosa_samantha Rh2AG062700 Rh2BG062000 Rh2CG063900 Rh2DG062300
rosa_wichuraiana Rw2G005740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1636
Acc16I TGCGCA 1 cut(s) 1151
Acc36I ACCTGC 1 cut(s) 1636
AccI GTMKAC 3 cut(s) 429, 933, 1218
AccII CGCG 8 cut(s) 107, 109, 153, 186, 488, 563, 1051, 1353
AccIII TCCGGA 1 cut(s) 1405
AclWI GGATC 4 cut(s) 1173, 1326, 1592, 1605
AcoI YGGCCR 2 cut(s) 590, 1046
AcsI RAATTY 4 cut(s) 10, 134, 641, 2275
AcuI CTGAAG 2 cut(s) 1590, 1833
AcyI GRCGYC 2 cut(s) 252, 521
AfaI GTAC 7 cut(s) 329, 506, 560, 928, 1205, 1801, 2367
AfiI CCNNNNNNNGG 5 cut(s) 58, 91, 599, 616, 722
AflII CTTAAG 1 cut(s) 1418
AgeI ACCGGT 1 cut(s) 43
AhdI GACNNNNNGTC 1 cut(s) 466
AhlI ACTAGT 2 cut(s) 1252, 1547
AjiI CACGTC 1 cut(s) 1654
AjnI CCWGG 5 cut(s) 743, 815, 1212, 1936, 2143
AleI CACNNNNGTG 1 cut(s) 1085
AloI GAACNNNNNNTCC 2 cut(s) 1582, 1614
Alw21I GWGCWC 1 cut(s) 2068
Alw26I GTCTC 5 cut(s) 229, 251, 294, 512, 1066
Alw44I GTGCAC 1 cut(s) 2064
AlwI GGATC 4 cut(s) 1173, 1326, 1592, 1605
Ama87I CYCGRG 2 cut(s) 400, 716
Aor13HI TCCGGA 1 cut(s) 1405
ApaI GGGCCC 2 cut(s) 610, 707
ApaLI GTGCAC 1 cut(s) 2064
ApeKI GCWGC 3 cut(s) 658, 804, 2009
ApoI RAATTY 4 cut(s) 10, 134, 641, 2275
ArsI GACNNNNNNTTYG 2 cut(s) 1252, 1284
AscI GGCGCGCC 1 cut(s) 486
AsiGI ACCGGT 1 cut(s) 43
Asp700I GAANNNNTTC 1 cut(s) 641
AsuC2I CCSGG 8 cut(s) 59, 312, 365, 548, 702, 717, 718, 1019
AsuHPI GGTGA 8 cut(s) 58, 400, 694, 1443, 1490, 1602, 1845, 2357
AvaI CYCGRG 2 cut(s) 400, 716
AvaII GGWCC 2 cut(s) 97, 725
AxyI CCTNAGG 1 cut(s) 1116
BaeGI GKGCMC 4 cut(s) 29, 610, 707, 2068
BamHI GGATCC 1 cut(s) 1597
BanII GRGCYC 3 cut(s) 610, 707, 1114
BarI GAAGNNNNNNTAC 2 cut(s) 1543, 1575
BbsI GAAGAC 1 cut(s) 1821
Bbv12I GWGCWC 1 cut(s) 2068
BbvI GCAGC 3 cut(s) 645, 791, 2021
BccI CCATC 4 cut(s) 281, 577, 836, 1609
BceAI ACGGC 6 cut(s) 314, 475, 499, 541, 550, 577
BcgI CGANNNNNNTGC 2 cut(s) 2378, 2412
BciT130I CCWGG 5 cut(s) 745, 817, 1214, 1938, 2145
BciVI GTATCC 1 cut(s) 1101
BcnI CCSGG 8 cut(s) 59, 312, 365, 548, 702, 717, 718, 1019
BcoDI GTCTC 5 cut(s) 229, 251, 294, 512, 1066
BcuI ACTAGT 2 cut(s) 1252, 1547
BfaI CTAG 9 cut(s) 354, 1095, 1187, 1253, 1548, 1886, 2169, 2201, 2354
BfmI CTRYAG 2 cut(s) 1735, 1968
BfoI RGCGCY 3 cut(s) 8, 148, 193
BfrI CTTAAG 1 cut(s) 1418
BfuAI ACCTGC 1 cut(s) 1636
BfuI GTATCC 1 cut(s) 1101
BlpI GCTNAGC 1 cut(s) 2400
BmcAI AGTACT 1 cut(s) 329
Bme18I GGWCC 2 cut(s) 97, 725
BmeRI GACNNNNNGTC 1 cut(s) 466
BmeT110I CYCGRG 2 cut(s) 400, 716
BmgBI CACGTC 1 cut(s) 1654
BmiI GGNNCC 9 cut(s) 238, 552, 608, 609, 705, 726, 1113, 1599, 1879
BmrI ACTGGG 1 cut(s) 1884
BmsI GCATC 7 cut(s) 414, 816, 1474, 1496, 1951, 2083, 2170
BmuI ACTGGG 1 cut(s) 1884
BpiI GAAGAC 1 cut(s) 1821
BpmI CTGGAG 1 cut(s) 993
Bpu1102I GCTNAGC 1 cut(s) 2400
BpuMI CCSGG 8 cut(s) 59, 312, 365, 548, 702, 717, 718, 1019
Bsa29I ATCGAT 1 cut(s) 2164
BsaHI GRCGYC 2 cut(s) 252, 521
BsaI GGTCTC 2 cut(s) 251, 1066
BsaWI WCCGGW 3 cut(s) 43, 432, 1405
BsaXI ACNNNNNCTCC 4 cut(s) 542, 572, 2047, 2077
Bsc4I CCNNNNNNNGG 5 cut(s) 58, 91, 599, 616, 722
Bse118I RCCGGY 5 cut(s) 43, 233, 588, 616, 688
Bse1I ACTGG 4 cut(s) 976, 1797, 1879, 2150
Bse21I CCTNAGG 1 cut(s) 1116
Bse3DI GCAATG 1 cut(s) 611
BseAI TCCGGA 1 cut(s) 1405
BseBI CCWGG 5 cut(s) 745, 817, 1214, 1938, 2145
BseCI ATCGAT 1 cut(s) 2164
BseGI GGATG 3 cut(s) 1281, 1601, 2098
BseLI CCNNNNNNNGG 5 cut(s) 58, 91, 599, 616, 722
BseMI GCAATG 1 cut(s) 611
BseMII CTCAG 3 cut(s) 1107, 1584, 2372
BseNI ACTGG 4 cut(s) 976, 1797, 1879, 2150
BsePI GCGCGC 1 cut(s) 486
BseRI GAGGAG 2 cut(s) 229, 764
BseSI GKGCMC 4 cut(s) 29, 610, 707, 2068
BseX3I CGGCCG 2 cut(s) 590, 1046
BseXI GCAGC 3 cut(s) 645, 791, 2021
BseYI CCCAGC 1 cut(s) 156
BsgI GTGCAG 3 cut(s) 713, 1602, 2028
Bsh1236I CGCG 8 cut(s) 107, 109, 153, 186, 488, 563, 1051, 1353
Bsh1285I CGRYCG 3 cut(s) 433, 593, 1049
BshTI ACCGGT 1 cut(s) 43
BshVI ATCGAT 1 cut(s) 2164
BsiEI CGRYCG 3 cut(s) 433, 593, 1049
BsiHKAI GWGCWC 1 cut(s) 2068
BsiHKCI CYCGRG 2 cut(s) 400, 716
BsiWI CGTACG 1 cut(s) 558
BslFI GGGAC 3 cut(s) 1310, 1885, 1918
BslI CCNNNNNNNGG 5 cut(s) 58, 91, 599, 616, 722
BsmAI GTCTC 5 cut(s) 229, 251, 294, 512, 1066
BsmBI CGTCTC 3 cut(s) 229, 294, 512
BsmFI GGGAC 3 cut(s) 1310, 1885, 1918
BsmI GAATGC 2 cut(s) 263, 1359
Bso31I GGTCTC 2 cut(s) 251, 1066
BsoBI CYCGRG 2 cut(s) 400, 716
Bsp120I GGGCCC 2 cut(s) 606, 703
Bsp1286I GDGCHC 5 cut(s) 29, 610, 707, 1114, 2068
Bsp13I TCCGGA 1 cut(s) 1405
Bsp1407I TGTACA 1 cut(s) 926
Bsp143I GATC 7 cut(s) 775, 1165, 1318, 1539, 1597, 2122, 2165
Bsp1720I GCTNAGC 1 cut(s) 2400
BspCNI CTCAG 3 cut(s) 1108, 1585, 2373
BspDI ATCGAT 1 cut(s) 2164
BspEI TCCGGA 1 cut(s) 1405
BspFNI CGCG 8 cut(s) 107, 109, 153, 186, 488, 563, 1051, 1353
BspLI GGNNCC 9 cut(s) 238, 552, 608, 609, 705, 726, 1113, 1599, 1879
BspMI ACCTGC 1 cut(s) 1636
BspPI GGATC 4 cut(s) 1173, 1326, 1592, 1605
BspTI CTTAAG 1 cut(s) 1418
BspTNI GGTCTC 2 cut(s) 251, 1066
BsrDI GCAATG 1 cut(s) 611
BsrFI RCCGGY 5 cut(s) 43, 233, 588, 616, 688
BsrGI TGTACA 1 cut(s) 926
BsrI ACTGG 4 cut(s) 976, 1797, 1879, 2150
BssAI RCCGGY 5 cut(s) 43, 233, 588, 616, 688
BssHII GCGCGC 1 cut(s) 486
BssMI GATC 7 cut(s) 775, 1165, 1318, 1539, 1597, 2122, 2165
BssNI GRCGYC 2 cut(s) 252, 521
BssT1I CCWWGG 1 cut(s) 1470
Bst2UI CCWGG 5 cut(s) 745, 817, 1214, 1938, 2145
Bst4CI ACNGT 8 cut(s) 223, 931, 1129, 1495, 1645, 1675, 1894, 2257
Bst6I CTCTTC 4 cut(s) 988, 1236, 1564, 2285
BstACI GRCGYC 2 cut(s) 252, 521
BstAFI CTTAAG 1 cut(s) 1418
BstAPI GCANNNNNTGC 1 cut(s) 900
BstAUI TGTACA 1 cut(s) 926
BstDEI CTNAG 5 cut(s) 729, 1116, 1593, 2381, 2400
BstDSI CCRYGG 4 cut(s) 90, 593, 1049, 2229
BstF5I GGATG 3 cut(s) 1281, 1601, 2098
BstFNI CGCG 8 cut(s) 107, 109, 153, 186, 488, 563, 1051, 1353
BstH2I RGCGCY 3 cut(s) 8, 148, 193
BstKTI GATC 7 cut(s) 778, 1168, 1321, 1542, 1600, 2125, 2168
BstMAI GTCTC 5 cut(s) 229, 251, 294, 512, 1066
BstMBI GATC 7 cut(s) 775, 1165, 1318, 1539, 1597, 2122, 2165
BstMCI CGRYCG 3 cut(s) 433, 593, 1049
BstNI CCWGG 5 cut(s) 745, 817, 1214, 1938, 2145
BstNSI RCATGY 1 cut(s) 862
BstSFI CTRYAG 2 cut(s) 1735, 1968
BstSLI GKGCMC 4 cut(s) 29, 610, 707, 2068
BstUI CGCG 8 cut(s) 107, 109, 153, 186, 488, 563, 1051, 1353
BstV1I GCAGC 3 cut(s) 645, 791, 2021
BstV2I GAAGAC 1 cut(s) 1821
BstX2I RGATCY 3 cut(s) 1165, 1318, 1597
BstXI CCANNNNNNTGG 1 cut(s) 2236
BstYI RGATCY 3 cut(s) 1165, 1318, 1597
BstZI CGGCCG 2 cut(s) 590, 1046
Bsu15I ATCGAT 1 cut(s) 2164
Bsu36I CCTNAGG 1 cut(s) 1116
BsuI GTATCC 1 cut(s) 1101
BsuTUI ATCGAT 1 cut(s) 2164
BtgI CCRYGG 4 cut(s) 90, 593, 1049, 2229
BtrI CACGTC 1 cut(s) 1654
BtsCI GGATG 3 cut(s) 1281, 1601, 2098
BtsI GCAGTG 1 cut(s) 1291
BtsIMutI CAGTG 4 cut(s) 1291, 1872, 1902, 2157
BveI ACCTGC 1 cut(s) 1636
Cfr10I RCCGGY 5 cut(s) 43, 233, 588, 616, 688
Cfr42I CCGCGG 1 cut(s) 1052
Cfr9I CCCGGG 1 cut(s) 716
ClaI ATCGAT 1 cut(s) 2164
CseI GACGC 3 cut(s) 260, 529, 1532
CsiI ACCWGGT 1 cut(s) 1212
Csp6I GTAC 7 cut(s) 328, 505, 559, 927, 1204, 1800, 2366
CspAI ACCGGT 1 cut(s) 43
CviAII CATG 7 cut(s) 859, 875, 937, 1063, 1838, 1927, 2412
CviQI GTAC 7 cut(s) 328, 505, 559, 927, 1204, 1800, 2366
DdeI CTNAG 5 cut(s) 729, 1116, 1593, 2381, 2400
DpnI GATC 7 cut(s) 777, 1167, 1320, 1541, 1599, 2124, 2167
DpnII GATC 7 cut(s) 775, 1165, 1318, 1539, 1597, 2122, 2165
DriI GACNNNNNGTC 1 cut(s) 466
EaeI YGGCCR 2 cut(s) 590, 1046
EagI CGGCCG 2 cut(s) 590, 1046
Eam1104I CTCTTC 4 cut(s) 988, 1236, 1564, 2285
Eam1105I GACNNNNNGTC 1 cut(s) 466
EarI CTCTTC 4 cut(s) 988, 1236, 1564, 2285
EciI GGCGGA 3 cut(s) 170, 193, 2130
EclXI CGGCCG 2 cut(s) 590, 1046
Eco130I CCWWGG 1 cut(s) 1470
Eco147I AGGCCT 2 cut(s) 1235, 1733
Eco24I GRGCYC 3 cut(s) 610, 707, 1114
Eco31I GGTCTC 2 cut(s) 251, 1066
Eco47I GGWCC 2 cut(s) 97, 725
Eco52I CGGCCG 2 cut(s) 590, 1046
Eco57I CTGAAG 2 cut(s) 1590, 1833
Eco81I CCTNAGG 1 cut(s) 1116
Eco88I CYCGRG 2 cut(s) 400, 716
EcoO109I RGGNCCY 2 cut(s) 607, 725
EcoRI GAATTC 1 cut(s) 641
EcoRII CCWGG 5 cut(s) 743, 815, 1212, 1936, 2143
EcoT14I CCWWGG 1 cut(s) 1470
EcoT22I ATGCAT 1 cut(s) 1489
EcoT38I GRGCYC 3 cut(s) 610, 707, 1114
ErhI CCWWGG 1 cut(s) 1470
Esp3I CGTCTC 3 cut(s) 229, 294, 512
FaeI CATG 7 cut(s) 862, 878, 940, 1066, 1841, 1930, 2415
FaqI GGGAC 3 cut(s) 1310, 1885, 1918
FatI CATG 7 cut(s) 858, 874, 936, 1062, 1837, 1926, 2411
FauI CCCGC 3 cut(s) 89, 93, 1101
FblI GTMKAC 3 cut(s) 429, 933, 1218
FokI GGATG 3 cut(s) 1288, 1588, 2105
FriOI GRGCYC 3 cut(s) 610, 707, 1114
FseI GGCCGGCC 1 cut(s) 592
FspBI CTAG 9 cut(s) 354, 1095, 1187, 1253, 1548, 1886, 2169, 2201, 2354
FspI TGCGCA 1 cut(s) 1151
GsaI CCCAGC 1 cut(s) 160
GsuI CTGGAG 1 cut(s) 993
HaeII RGCGCY 3 cut(s) 8, 148, 193
HgaI GACGC 3 cut(s) 260, 529, 1532
Hin1I GRCGYC 2 cut(s) 252, 521
Hin1II CATG 7 cut(s) 862, 878, 940, 1066, 1841, 1930, 2415
HincII GTYRAC 3 cut(s) 430, 771, 934
HindII GTYRAC 3 cut(s) 430, 771, 934
HindIII AAGCTT 1 cut(s) 1848
HphI GGTGA 8 cut(s) 58, 400, 694, 1443, 1490, 1602, 1845, 2357
Hpy188III TCNNGA 9 cut(s) 400, 779, 811, 1115, 1406, 1532, 1806, 2038, 2354
Hpy99I CGWCG 7 cut(s) 233, 254, 461, 464, 485, 1655, 2089
HpyAV CCTTC 9 cut(s) 294, 312, 631, 1150, 1464, 1469, 1617, 1723, 2299
HpyCH4III ACNGT 8 cut(s) 223, 931, 1129, 1495, 1645, 1675, 1894, 2257
HpyCH4IV ACGT 2 cut(s) 474, 1653
HpyF3I CTNAG 5 cut(s) 729, 1116, 1593, 2381, 2400
HpySE526I ACGT 2 cut(s) 474, 1653
Hsp92I GRCGYC 2 cut(s) 252, 521
Hsp92II CATG 7 cut(s) 862, 878, 940, 1066, 1841, 1930, 2415
Kpn2I TCCGGA 1 cut(s) 1405
KroI GCCGGC 3 cut(s) 233, 588, 616
KroNI GCCGGC 3 cut(s) 235, 590, 618
KspI CCGCGG 1 cut(s) 1052
Kzo9I GATC 7 cut(s) 775, 1165, 1318, 1539, 1597, 2122, 2165
LmnI GCTCC 6 cut(s) 43, 242, 549, 550, 1117, 2143
Lsp1109I GCAGC 3 cut(s) 645, 791, 2021
LweI GCATC 7 cut(s) 414, 816, 1474, 1496, 1951, 2083, 2170
MabI ACCWGGT 1 cut(s) 1212
MaeI CTAG 9 cut(s) 354, 1095, 1187, 1253, 1548, 1886, 2169, 2201, 2354
MaeII ACGT 2 cut(s) 474, 1653
MaeIII GTNAC 6 cut(s) 294, 1286, 1675, 1888, 1943, 2031
MalI GATC 7 cut(s) 777, 1167, 1320, 1541, 1599, 2124, 2167
MboI GATC 7 cut(s) 775, 1165, 1318, 1539, 1597, 2122, 2165
MfeI CAATTG 2 cut(s) 1458, 1563
MflI RGATCY 3 cut(s) 1165, 1318, 1597
MhlI GDGCHC 5 cut(s) 29, 610, 707, 1114, 2068
MlyI GAGTC 9 cut(s) 194, 387, 641, 1036, 1442, 1790, 1940, 1945, 2394
MmeI TCCRAC 4 cut(s) 273, 1674, 2070, 2217
Mph1103I ATGCAT 1 cut(s) 1489
MroI TCCGGA 1 cut(s) 1405
MroNI GCCGGC 3 cut(s) 233, 588, 616
MroXI GAANNNNTTC 1 cut(s) 641
MseI TTAA 6 cut(s) 540, 798, 909, 1313, 1419, 2157
MslI CAYNNNNRTG 3 cut(s) 1085, 1364, 1842
MspA1I CMGCKG 3 cut(s) 658, 1051, 1108
MspCI CTTAAG 1 cut(s) 1418
MunI CAATTG 2 cut(s) 1458, 1563
Mva1269I GAATGC 2 cut(s) 263, 1359
MvaI CCWGG 5 cut(s) 745, 817, 1214, 1938, 2145
MvnI CGCG 8 cut(s) 107, 109, 153, 186, 488, 563, 1051, 1353
NaeI GCCGGC 3 cut(s) 235, 590, 618
NciI CCSGG 8 cut(s) 59, 312, 365, 548, 702, 717, 718, 1019
NdeII GATC 7 cut(s) 775, 1165, 1318, 1539, 1597, 2122, 2165
NgoMIV GCCGGC 3 cut(s) 233, 588, 616
NlaIII CATG 7 cut(s) 862, 878, 940, 1066, 1841, 1930, 2415
NlaIV GGNNCC 9 cut(s) 238, 552, 608, 609, 705, 726, 1113, 1599, 1879
NmeAIII GCCGAG 4 cut(s) 154, 280, 401, 1024
NmuCI GTSAC 3 cut(s) 294, 1286, 2031
NsbI TGCGCA 1 cut(s) 1151
NsiI ATGCAT 1 cut(s) 1489
NspI RCATGY 1 cut(s) 862
OliI CACNNNNGTG 1 cut(s) 1085
PaeR7I CTCGAG 1 cut(s) 400
PalAI GGCGCGCC 1 cut(s) 486
PaqCI CACCTGC 1 cut(s) 1636
PauI GCGCGC 1 cut(s) 486
PceI AGGCCT 2 cut(s) 1235, 1733
PcsI WCGNNNNNNNCGW 4 cut(s) 126, 365, 398, 704
PctI GAATGC 2 cut(s) 263, 1359
PdiI GCCGGC 3 cut(s) 235, 590, 618
PdmI GAANNNNTTC 1 cut(s) 641
PfeI GAWTC 6 cut(s) 102, 213, 1102, 1448, 2081, 2309
Pfl23II CGTACG 1 cut(s) 558
PflFI GACNNNGTC 1 cut(s) 1223
PfoI TCCNGGA 1 cut(s) 546
PinAI ACCGGT 1 cut(s) 43
PleI GAGTC 9 cut(s) 194, 386, 641, 1036, 1441, 1789, 1939, 1945, 2393
PpsI GAGTC 9 cut(s) 194, 386, 641, 1036, 1441, 1789, 1939, 1945, 2393
PpuMI RGGWCCY 1 cut(s) 725
Psp5II RGGWCCY 1 cut(s) 725
Psp6I CCWGG 5 cut(s) 743, 815, 1212, 1936, 2143
PspFI CCCAGC 1 cut(s) 156
PspGI CCWGG 5 cut(s) 743, 815, 1212, 1936, 2143
PspLI CGTACG 1 cut(s) 558
PspN4I GGNNCC 9 cut(s) 238, 552, 608, 609, 705, 726, 1113, 1599, 1879
PspOMI GGGCCC 2 cut(s) 606, 703
PspPPI RGGWCCY 1 cut(s) 725
PsrI GAACNNNNNNTAC 2 cut(s) 551, 583
PsuI RGATCY 3 cut(s) 1165, 1318, 1597
PsyI GACNNNGTC 1 cut(s) 1223
PteI GCGCGC 1 cut(s) 486
PvuII CAGCTG 1 cut(s) 658
RigI GGCCGGCC 1 cut(s) 592
RsaI GTAC 7 cut(s) 329, 506, 560, 928, 1205, 1801, 2367
RsaNI GTAC 7 cut(s) 328, 505, 559, 927, 1204, 1800, 2366
RseI CAYNNNNRTG 3 cut(s) 1085, 1364, 1842
SacII CCGCGG 1 cut(s) 1052
SalI GTCGAC 2 cut(s) 428, 932
SaqAI TTAA 6 cut(s) 540, 798, 909, 1313, 1419, 2157
Sau3AI GATC 7 cut(s) 775, 1165, 1318, 1539, 1597, 2122, 2165
ScaI AGTACT 1 cut(s) 329
SchI GAGTC 9 cut(s) 194, 387, 641, 1036, 1442, 1790, 1940, 1945, 2394
SduI GDGCHC 5 cut(s) 29, 610, 707, 1114, 2068
SexAI ACCWGGT 1 cut(s) 1212
SfaNI GCATC 7 cut(s) 414, 816, 1474, 1496, 1951, 2083, 2170
SfcI CTRYAG 2 cut(s) 1735, 1968
Sfr274I CTCGAG 1 cut(s) 400
Sfr303I CCGCGG 1 cut(s) 1052
SgrAI CRCCGGYG 1 cut(s) 43
SgrBI CCGCGG 1 cut(s) 1052
SgsI GGCGCGCC 1 cut(s) 486
SinI GGWCC 2 cut(s) 97, 725
SlaI CTCGAG 1 cut(s) 400
SmaI CCCGGG 1 cut(s) 718
SmiMI CAYNNNNRTG 3 cut(s) 1085, 1364, 1842
SmlI CTYRAG 2 cut(s) 400, 1418
SmoI CTYRAG 2 cut(s) 400, 1418
SpeI ACTAGT 2 cut(s) 1252, 1547
SseBI AGGCCT 2 cut(s) 1235, 1733
SspI AATATT 1 cut(s) 871
SspMI CTAG 9 cut(s) 354, 1095, 1187, 1253, 1548, 1886, 2169, 2201, 2354
StuI AGGCCT 2 cut(s) 1235, 1733
StyI CCWWGG 1 cut(s) 1470
TaaI ACNGT 8 cut(s) 223, 931, 1129, 1495, 1645, 1675, 1894, 2257
TaiI ACGT 2 cut(s) 477, 1656
TaqII GACCGA 1 cut(s) 788
TatI WGTACW 5 cut(s) 327, 504, 926, 1203, 1799
TfiI GAWTC 6 cut(s) 102, 213, 1102, 1448, 2081, 2309
Tru1I TTAA 6 cut(s) 540, 798, 909, 1313, 1419, 2157
Tru9I TTAA 6 cut(s) 540, 798, 909, 1313, 1419, 2157
TscAI CASTG 4 cut(s) 1291, 1872, 1909, 2157
TseFI GTSAC 3 cut(s) 294, 1286, 2031
TseI GCWGC 3 cut(s) 658, 804, 2009
Tsp45I GTSAC 3 cut(s) 294, 1286, 2031
TspDTI ATGAA 8 cut(s) 650, 863, 1292, 1725, 1742, 1860, 2058, 2406
TspGWI ACGGA 4 cut(s) 107, 217, 282, 2246
TspMI CCCGGG 1 cut(s) 716
TspRI CASTG 4 cut(s) 1291, 1872, 1909, 2157
Tth111I GACNNNGTC 1 cut(s) 1223
Vha464I CTTAAG 1 cut(s) 1418
VneI GTGCAC 1 cut(s) 2064
VpaK11BI GGWCC 2 cut(s) 97, 725
XapI RAATTY 4 cut(s) 10, 134, 641, 2275
XbaI TCTAGA 1 cut(s) 2353
XceI RCATGY 1 cut(s) 862
XhoI CTCGAG 1 cut(s) 400
XmaI CCCGGG 1 cut(s) 716
XmiI GTMKAC 3 cut(s) 429, 933, 1218
XmnI GAANNNNTTC 1 cut(s) 641
XspI CTAG 9 cut(s) 354, 1095, 1187, 1253, 1548, 1886, 2169, 2201, 2354
ZrmI AGTACT 1 cut(s) 329
Zsp2I ATGCAT 1 cut(s) 1489
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.