AT2G20585

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0001863
Physical Location & Seq
Forward (+)
8865016 .. 8866791
1776 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G20585.2

Sequence Viewer

Length: 303 bp
ATGGCCACGTCCGCCGTCCTCTCCGGTGCCAGATCGATGCTTCGAGCTGCTTCCTCACGCAGCGCCGCTGCTTCTACTGGCCGCTTCGCCTCTCAAGCGAAATCCGCTCCACCATTGTTTAGAGCCACTGCCAGAAGAAGCCCACTGCTTTCTCCTCTCCGAAATCCTGTGGAACTGAGCTTCTGTGTGGAGTCATTGTTACCATATCACTCGGCTACAGCTTCAGCGCTAATGACTTCAAAGCTTTCTATCTCTGGCCAAACCTATGGCTGGCTCTCTGACGCTTGCAATGATGATGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000741 GO:0006996 GO:0006997 GO:0007275 GO:0008150 GO:0009553 GO:0009559 GO:0009561 GO:0009987 GO:0010197 GO:0016043 GO:0030154 GO:0032501 GO:0032502 GO:0048229 GO:0048284 GO:0048856 GO:0048869 GO:0071840
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

100

Amino Acids

10.45

Weight (kDa)

10.04

Isoelectric Point (pI)

62.7

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 26
AccBSI CCGCTC 1 cut(s) 107
AciI CCGC 4 cut(s) 12, 66, 82, 105
AcoI YGGCCR 3 cut(s) 3, 79, 256
AcuI CTGAAG 1 cut(s) 207
AfeI AGCGCT 1 cut(s) 228
AfiI CCNNNNNNNGG 1 cut(s) 270
AgsI TTSAA 1 cut(s) 240
AjiI CACGTC 1 cut(s) 9
AluBI AGCT 4 cut(s) 47, 180, 221, 244
AluI AGCT 4 cut(s) 47, 180, 221, 244
Aor51HI AGCGCT 1 cut(s) 228
AoxI GGCC 3 cut(s) 3, 79, 256
ApeKI GCWGC 3 cut(s) 47, 60, 68
AspLEI GCGC 2 cut(s) 65, 229
BalI TGGCCA 2 cut(s) 5, 258
BanI GGYRCC 1 cut(s) 26
BbvI GCAGC 3 cut(s) 34, 55, 72
BfmI CTRYAG 1 cut(s) 216
BfoI RGCGCY 2 cut(s) 66, 230
BisI GCNGC 5 cut(s) 48, 61, 66, 69, 82
BlsI GCNGC 5 cut(s) 49, 62, 67, 70, 83
BmgBI CACGTC 1 cut(s) 9
BmiI GGNNCC 1 cut(s) 28
BmsI GCATC 1 cut(s) 27
BpuEI CTTGAG 1 cut(s) 78
Bsa29I ATCGAT 1 cut(s) 35
BsaWI WCCGGW 1 cut(s) 23
BsaXI ACNNNNNCTCC 2 cut(s) 182, 212
Bsc4I CCNNNNNNNGG 1 cut(s) 270
Bse1I ACTGG 1 cut(s) 82
Bse3DI GCAATG 1 cut(s) 295
BseCI ATCGAT 1 cut(s) 35
BseLI CCNNNNNNNGG 1 cut(s) 270
BseMI GCAATG 1 cut(s) 295
BseMII CTCAG 1 cut(s) 167
BseNI ACTGG 1 cut(s) 82
BseRI GAGGAG 1 cut(s) 144
BseXI GCAGC 3 cut(s) 34, 55, 72
BshFI GGCC 3 cut(s) 5, 81, 258
BshNI GGYRCC 1 cut(s) 26
BshVI ATCGAT 1 cut(s) 35
BsiSI CCGG 1 cut(s) 24
BslI CCNNNNNNNGG 1 cut(s) 270
BsnI GGCC 3 cut(s) 5, 81, 258
Bsp143I GATC 1 cut(s) 32
BspACI CCGC 4 cut(s) 12, 66, 82, 105
BspANI GGCC 3 cut(s) 5, 81, 258
BspCNI CTCAG 1 cut(s) 168
BspDI ATCGAT 1 cut(s) 35
BspLI GGNNCC 1 cut(s) 28
BspT107I GGYRCC 1 cut(s) 26
BsrBI CCGCTC 1 cut(s) 107
BsrDI GCAATG 1 cut(s) 295
BsrI ACTGG 1 cut(s) 82
BssMI GATC 1 cut(s) 32
BstC8I GCNNGC 2 cut(s) 272, 286
BstDEI CTNAG 1 cut(s) 176
BstH2I RGCGCY 2 cut(s) 66, 230
BstHHI GCGC 2 cut(s) 65, 229
BstKTI GATC 1 cut(s) 35
BstMBI GATC 1 cut(s) 32
BstMWI GCNNNNNNNGC 3 cut(s) 11, 95, 104
BstSFI CTRYAG 1 cut(s) 216
BstV1I GCAGC 3 cut(s) 34, 55, 72
BstXI CCANNNNNNTGG 1 cut(s) 266
Bsu15I ATCGAT 1 cut(s) 35
BsuRI GGCC 3 cut(s) 5, 81, 258
BsuTUI ATCGAT 1 cut(s) 35
BtrI CACGTC 1 cut(s) 9
BtsI GCAGTG 2 cut(s) 126, 143
BtsIMutI CAGTG 2 cut(s) 126, 143
Cac8I GCNNGC 2 cut(s) 272, 286
CfoI GCGC 2 cut(s) 65, 229
ClaI ATCGAT 1 cut(s) 35
CseI GACGC 1 cut(s) 290
DdeI CTNAG 1 cut(s) 176
DpnI GATC 1 cut(s) 34
DpnII GATC 1 cut(s) 32
EaeI YGGCCR 3 cut(s) 3, 79, 256
Eco47III AGCGCT 1 cut(s) 228
Eco57I CTGAAG 1 cut(s) 207
FaiI YATR 2 cut(s) 205, 267
Fnu4HI GCNGC 5 cut(s) 48, 61, 66, 69, 82
Fsp4HI GCNGC 5 cut(s) 48, 61, 66, 69, 82
GlaI GCGC 2 cut(s) 64, 228
GluI GCNGC 5 cut(s) 48, 61, 66, 69, 82
HaeII RGCGCY 2 cut(s) 66, 230
HaeIII GGCC 3 cut(s) 5, 81, 258
HapII CCGG 1 cut(s) 24
HgaI GACGC 1 cut(s) 290
HhaI GCGC 2 cut(s) 65, 229
Hin6I GCGC 2 cut(s) 63, 227
HinP1I GCGC 2 cut(s) 63, 227
HindIII AAGCTT 1 cut(s) 242
HinfI GANTC 1 cut(s) 191
HpaII CCGG 1 cut(s) 24
Hpy188I TCNGA 2 cut(s) 161, 280
HpyCH4IV ACGT 1 cut(s) 8
HpyCH4V TGCA 1 cut(s) 288
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 95, 104
HpyF3I CTNAG 1 cut(s) 176
HpySE526I ACGT 1 cut(s) 8
HspAI GCGC 2 cut(s) 63, 227
Kzo9I GATC 1 cut(s) 32
LmnI GCTCC 1 cut(s) 112
LpnPI CCDG 7 cut(s) 37, 43, 63, 145, 180, 240, 256
Lsp1109I GCAGC 3 cut(s) 34, 55, 72
LweI GCATC 1 cut(s) 27
MaeII ACGT 1 cut(s) 8
MaeIII GTNAC 1 cut(s) 198
MalI GATC 1 cut(s) 34
MbiI CCGCTC 1 cut(s) 107
MboI GATC 1 cut(s) 32
MboII GAAGA 1 cut(s) 147
MlsI TGGCCA 2 cut(s) 5, 258
MluNI TGGCCA 2 cut(s) 5, 258
MlyI GAGTC 1 cut(s) 200
MnlI CCTC 4 cut(s) 29, 64, 100, 165
Mox20I TGGCCA 2 cut(s) 5, 258
MscI TGGCCA 2 cut(s) 5, 258
Msp20I TGGCCA 2 cut(s) 5, 258
MspA1I CMGCKG 1 cut(s) 68
MspI CCGG 1 cut(s) 24
MwoI GCNNNNNNNGC 3 cut(s) 11, 95, 104
NdeII GATC 1 cut(s) 32
NlaIV GGNNCC 1 cut(s) 28
NmeAIII GCCGAG 1 cut(s) 191
PkrI GCNGC 5 cut(s) 49, 62, 67, 70, 83
PleI GAGTC 1 cut(s) 199
PpsI GAGTC 1 cut(s) 199
PspN4I GGNNCC 1 cut(s) 28
SatI GCNGC 5 cut(s) 48, 61, 66, 69, 82
Sau3AI GATC 1 cut(s) 32
SchI GAGTC 1 cut(s) 200
SetI ASST 6 cut(s) 11, 49, 182, 223, 246, 266
SfaNI GCATC 1 cut(s) 27
SfcI CTRYAG 1 cut(s) 216
SmlI CTYRAG 1 cut(s) 93
SmoI CTYRAG 1 cut(s) 93
SsiI CCGC 4 cut(s) 12, 66, 82, 105
TaiI ACGT 1 cut(s) 11
TaqI TCGA 2 cut(s) 35, 43
TauI GCSGC 2 cut(s) 68, 84
TscAI CASTG 2 cut(s) 133, 150
TseI GCWGC 3 cut(s) 47, 60, 68
TspRI CASTG 2 cut(s) 133, 150
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.