Rorug02G0275200

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0001863
Physical Location & Seq
Reverse (-)
29463128 .. 29471201
8074 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0275200.1

Sequence Viewer

Length: 1713 bp
ATGGACTTCGACGAGTACGATTACCTGGAGAAGACCGTGCAGGAGAACGACGACCGTGACTCGAAGAAGAAGGCGAAGAAGGAAGCCACCGAGAAGAGCTACAGGAAGAGAGAGGTAGACGACGACGTTTCGGCCGACGAGGATAGGAAGAGCAGCAAGAGGTCCAGAGGCGGCGAGCACGAGAATGGCCGCGATAAGGAAAAGGATAGGGATCGGCACCACCGGAGCAGCGGCAGTGGTAGAGAGAGGGACAGAGACGGCGAGAGGGACCGCGATCGTGATCGCGGCGGCGACCGAGAGAGGGAGAGGAGCAGTAGAGAGAAGGAGAAGGAGAGAGAAAGGAGGGATAGGGATAAGGAGAAGGAGAAGGAGAGAGAGAAAGAGAAGGAGAAAGAAAGAGAGAGGAGGGAGAGGAGAGAGAGGGATGATAGAGAGAGAAGAGAGAAGGATGAGAAGGAGAGGTCTCGGAGGAGTAGGAGCCACACTGATCGTGATAGGGAGAGGGAAGTTAGGGAAAAGGAACGAGAATTCGAGATGAATGAGAGCAGGAGGTTTAAAGACAAAAAAGAAATGGTGGAGCCTGAAGCTGATCCAGAAAGGGATCAGAGAACTGTTTTTGCTTACCAGATGCCACTGAAGGCGACCGAGCGAGATGTTTATGAATTCTTCTCAAAGGCGGGCAAGGTGAGGGATGTACGGCTGATCATGGACAGGAATTCTAGACGATCGAAAGGTGTTGGGTATATTGAATTTTATGATGCAATGCATGTGCCGATGGCTATAGCTCTCTCTGGTCAGCTACTTCTTGGACAACCTGTAATGGTGAAACCTTCAGAGGCAGAAAAGAATCTTGTTCAATCTACTGCTACTGGTGGTACCACAGGCGTAGTAGGACCATATGGTGCAGTTGACAGGAAACTTTATGTAGGGAACTTGCATTTTAACATGACTGAGACTCATCTTAGAGAGATTTTTGAACCATTTGGTCCAGTGGAGCTCGTGCAACTTCCCCTTGATATTGAGACATCACAGTGCAAGGGCTTTGGCTTTGTTCAATTTGCTCATCTGGAACATGCAAAGGCAGCTCAAAGTTTGAATGGGAAGTTGGACATTGCTGGTCGAACTATCAAGGTTTCATCCGTTACAGATCATGTTGGCTCACAAGAGACTGGTGCAAAATCAGCAGATTTTGATGATGATGATGGGGGTGGTTTGTCTTTAAATGCTCAATCTAGAGCATTGCTAATGCAAAAGCTGGACCGCACAGGAACTGCCACAAGTATTGCAGGTTCTCTAGGAGTACCTGGGCTAAATGGGTCAGCACCTAATCAACGAGCTGTTAATTTGCCGATGAATGGTCAAGCCACCCTTTCTGCACCATTTCTTCCAGCAAAAGTGCCTGCAGTAGCAGAACCTATTGGAAACCCCAGTGAATGTCTATTATTGAAGGATATGTTTGATCCAGCCACGGAGACGGAGCCAGATTTTGATGTAGACATAAAAGATGACGTTGAAGATGAATGTTCAAAATATGGTCGCGTGAAGCACATTTATGTGGAGAAAAACAGTGCTGGATTTGTGTATTTGCGGTTTGAAACTGTGGAAGCAGCAGCTGCAGCTCAACGTGCAATGCATCTGAGATGGTTTGCAGGCAGGCAGATTTCAGCCCTTTTCATGCAACCTCAAGTTTACGAGGCCAAGTTTGGAGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000741 GO:0006996 GO:0006997 GO:0007275 GO:0008150 GO:0009553 GO:0009559 GO:0009561 GO:0009987 GO:0010197 GO:0016043 GO:0030154 GO:0032501 GO:0032502 GO:0048229 GO:0048284 GO:0048856 GO:0048869 GO:0071840
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

570

Amino Acids

65.34

Weight (kDa)

6.19

Isoelectric Point (pI)

43.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_1 PF00076 210 - 272 1.6e-10 RNA recognition motif
RRM_1 PF00076 307 - 377 4.8e-19 RNA recognition motif
RBM39linker PF15519 397 - 496 1.6e-21 linker between RRM2 and RRM3 domains in RBM39 protein
RRM_1 PF00076 502 - 554 3.8e-07 RNA recognition motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1277
Acc65I GGTACC 1 cut(s) 875
AccB1I GGYRCC 2 cut(s) 216, 875
AccI GTMKAC 2 cut(s) 117, 1494
AccII CGCG 4 cut(s) 192, 273, 285, 1539
AciI CCGC 9 cut(s) 171, 190, 231, 271, 285, 288, 677, 1261, 1588
AclWI GGATC 4 cut(s) 219, 584, 609, 1454
AcoI YGGCCR 2 cut(s) 132, 187
AcsI RAATTY 4 cut(s) 527, 662, 715, 749
AcuI CTGAAG 3 cut(s) 603, 656, 816
AfaI GTAC 4 cut(s) 17, 696, 877, 1302
AfiI CCNNNNNNNGG 3 cut(s) 196, 301, 1355
AgsI TTSAA 9 cut(s) 749, 857, 977, 1055, 1096, 1447, 1514, 1527, 1595
AjnI CCWGG 2 cut(s) 24, 1303
AjuI GAANNNNNNNTTGG 2 cut(s) 1088, 1120
Alw21I GWGCWC 2 cut(s) 180, 999
Alw26I GTCTC 6 cut(s) 249, 468, 947, 1016, 1160, 1466
AlwI GGATC 4 cut(s) 219, 584, 609, 1454
AlwNI CAGNNNCTG 2 cut(s) 1271, 1613
AoxI GGCC 3 cut(s) 132, 187, 1695
ApeKI GCWGC 7 cut(s) 153, 228, 1082, 1607, 1610, 1613, 1616
ApoI RAATTY 4 cut(s) 527, 662, 715, 749
Asp718I GGTACC 1 cut(s) 875
AspS9I GGNCC 5 cut(s) 162, 268, 893, 986, 1258
AsuHPI GGTGA 2 cut(s) 697, 835
AvaII GGWCC 5 cut(s) 162, 268, 893, 986, 1258
BanI GGYRCC 2 cut(s) 216, 875
BanII GRGCYC 1 cut(s) 999
BauI CACGAG 2 cut(s) 179, 998
BbsI GAAGAC 1 cut(s) 38
Bbv12I GWGCWC 2 cut(s) 180, 999
BbvI GCAGC 7 cut(s) 165, 240, 1094, 1600, 1619, 1622, 1628
BccI CCATC 3 cut(s) 769, 1196, 1635
BceAI ACGGC 2 cut(s) 274, 713
BciT130I CCWGG 2 cut(s) 26, 1305
BclI TGATCA 1 cut(s) 702
BcoDI GTCTC 6 cut(s) 249, 468, 947, 1016, 1160, 1466
BfaI CTAG 3 cut(s) 720, 1233, 1295
BfmI CTRYAG 4 cut(s) 100, 780, 1401, 1614
BfuAI ACCTGC 1 cut(s) 1277
Bme1390I CCNGG 2 cut(s) 26, 1305
Bme18I GGWCC 5 cut(s) 162, 268, 893, 986, 1258
BmgT120I GGNCC 5 cut(s) 162, 268, 893, 986, 1258
BmiI GGNNCC 6 cut(s) 218, 269, 479, 579, 877, 1479
BmrFI CCNGG 2 cut(s) 26, 1305
BmrI ACTGGG 1 cut(s) 1422
BmsI GCATC 3 cut(s) 618, 748, 1642
BmuI ACTGGG 1 cut(s) 1422
BpiI GAAGAC 1 cut(s) 38
BpmI CTGGAG 1 cut(s) 47
BpuEI CTTGAG 1 cut(s) 1668
BsaBI GATNNNNATC 2 cut(s) 210, 279
BsaI GGTCTC 1 cut(s) 468
BsaJI CCNNGG 2 cut(s) 1304, 1467
BsaWI WCCGGW 1 cut(s) 222
Bsc4I CCNNNNNNNGG 3 cut(s) 196, 301, 1355
Bse1I ACTGG 4 cut(s) 874, 989, 1174, 1428
Bse3DI GCAATG 4 cut(s) 768, 1110, 1238, 1635
Bse8I GATNNNNATC 2 cut(s) 210, 279
BseBI CCWGG 2 cut(s) 26, 1305
BseDI CCNNGG 2 cut(s) 1304, 1467
BseGI GGATG 4 cut(s) 430, 454, 697, 1136
BseJI GATNNNNATC 2 cut(s) 210, 279
BseLI CCNNNNNNNGG 3 cut(s) 196, 301, 1355
BseMI GCAATG 4 cut(s) 768, 1110, 1238, 1635
BseMII CTCAG 2 cut(s) 942, 1628
BseNI ACTGG 4 cut(s) 874, 989, 1174, 1428
BseRI GAGGAG 4 cut(s) 322, 418, 427, 484
BseX3I CGGCCG 1 cut(s) 132
BseXI GCAGC 7 cut(s) 165, 240, 1094, 1600, 1619, 1622, 1628
BsgI GTGCAG 3 cut(s) 59, 924, 1359
Bsh1236I CGCG 4 cut(s) 192, 273, 285, 1539
Bsh1285I CGRYCG 6 cut(s) 55, 135, 277, 295, 645, 728
BshFI GGCC 3 cut(s) 134, 189, 1697
BshNI GGYRCC 2 cut(s) 216, 875
BsiEI CGRYCG 6 cut(s) 55, 135, 277, 295, 645, 728
BsiHKAI GWGCWC 2 cut(s) 180, 999
BsiSI CCGG 1 cut(s) 223
BslFI GGGAC 2 cut(s) 263, 281
BslI CCNNNNNNNGG 3 cut(s) 196, 301, 1355
BsmAI GTCTC 6 cut(s) 249, 468, 947, 1016, 1160, 1466
BsmBI CGTCTC 2 cut(s) 249, 1466
BsmFI GGGAC 2 cut(s) 263, 281
BsnI GGCC 3 cut(s) 134, 189, 1697
Bso31I GGTCTC 1 cut(s) 468
Bsp1286I GDGCHC 2 cut(s) 180, 999
BspACI CCGC 9 cut(s) 171, 190, 231, 271, 285, 288, 677, 1261, 1588
BspANI GGCC 3 cut(s) 134, 189, 1697
BspCNI CTCAG 2 cut(s) 943, 1629
BspFNI CGCG 4 cut(s) 192, 273, 285, 1539
BspLI GGNNCC 6 cut(s) 218, 269, 479, 579, 877, 1479
BspMAI CTGCAG 2 cut(s) 1405, 1618
BspMI ACCTGC 1 cut(s) 1277
BspPI GGATC 4 cut(s) 219, 584, 609, 1454
BspQI GCTCTTC 2 cut(s) 89, 143
BspT107I GGYRCC 2 cut(s) 216, 875
BspTNI GGTCTC 1 cut(s) 468
BsrDI GCAATG 4 cut(s) 768, 1110, 1238, 1635
BsrI ACTGG 4 cut(s) 874, 989, 1174, 1428
BssECI CCNNGG 2 cut(s) 1304, 1467
BssSI CACGAG 2 cut(s) 179, 998
Bst2BI CACGAG 2 cut(s) 179, 998
Bst2UI CCWGG 2 cut(s) 26, 1305
Bst4CI ACNGT 6 cut(s) 37, 56, 613, 1032, 1568, 1600
Bst6I CTCTTC 4 cut(s) 89, 101, 143, 433
BstAPI GCANNNNNTGC 1 cut(s) 1613
BstC8I GCNNGC 5 cut(s) 176, 679, 1401, 1651, 1655
BstDEI CTNAG 3 cut(s) 951, 962, 1637
BstDSI CCRYGG 1 cut(s) 1467
BstF5I GGATG 4 cut(s) 430, 454, 697, 1136
BstFNI CGCG 4 cut(s) 192, 273, 285, 1539
BstMAI GTCTC 6 cut(s) 249, 468, 947, 1016, 1160, 1466
BstMCI CGRYCG 6 cut(s) 55, 135, 277, 295, 645, 728
BstMWI GCNNNNNNNGC 5 cut(s) 1082, 1181, 1613, 1616, 1625
BstNI CCWGG 2 cut(s) 26, 1305
BstNSI RCATGY 2 cut(s) 770, 1076
BstSCI CCNGG 2 cut(s) 24, 1303
BstSFI CTRYAG 4 cut(s) 100, 780, 1401, 1614
BstUI CGCG 4 cut(s) 192, 273, 285, 1539
BstV1I GCAGC 7 cut(s) 165, 240, 1094, 1600, 1619, 1622, 1628
BstV2I GAAGAC 1 cut(s) 38
BstZI CGGCCG 1 cut(s) 132
BsuRI GGCC 3 cut(s) 134, 189, 1697
BtgI CCRYGG 1 cut(s) 1467
BtsCI GGATG 4 cut(s) 430, 454, 697, 1136
BtsI GCAGTG 1 cut(s) 241
BtsIMutI CAGTG 7 cut(s) 241, 483, 632, 996, 1037, 1435, 1573
BveI ACCTGC 1 cut(s) 1277
Cac8I GCNNGC 5 cut(s) 176, 679, 1401, 1651, 1655
CaiI CAGNNNCTG 2 cut(s) 1271, 1613
Cfr13I GGNCC 5 cut(s) 162, 268, 893, 986, 1258
Csp6I GTAC 4 cut(s) 16, 695, 876, 1301
CspCI CAANNNNNGTGG 2 cut(s) 1264, 1299
CviAII CATG 6 cut(s) 706, 767, 946, 1073, 1151, 1675
CviQI GTAC 4 cut(s) 16, 695, 876, 1301
DdeI CTNAG 3 cut(s) 951, 962, 1637
DraI TTTAAA 2 cut(s) 556, 1221
EaeI YGGCCR 2 cut(s) 132, 187
EagI CGGCCG 1 cut(s) 132
Eam1104I CTCTTC 4 cut(s) 89, 101, 143, 433
EarI CTCTTC 4 cut(s) 89, 101, 143, 433
Ecl136II GAGCTC 1 cut(s) 997
EclXI CGGCCG 1 cut(s) 132
Eco24I GRGCYC 1 cut(s) 999
Eco31I GGTCTC 1 cut(s) 468
Eco47I GGWCC 5 cut(s) 162, 268, 893, 986, 1258
Eco52I CGGCCG 1 cut(s) 132
Eco53kI GAGCTC 1 cut(s) 997
Eco57I CTGAAG 3 cut(s) 603, 656, 816
EcoICRI GAGCTC 1 cut(s) 997
EcoRI GAATTC 3 cut(s) 527, 662, 715
EcoRII CCWGG 2 cut(s) 24, 1303
EcoT22I ATGCAT 2 cut(s) 768, 1635
EcoT38I GRGCYC 1 cut(s) 999
Esp3I CGTCTC 2 cut(s) 249, 1466
FaeI CATG 6 cut(s) 709, 770, 949, 1076, 1154, 1678
FalI AAGNNNNNCTT 2 cut(s) 1353, 1385
FaqI GGGAC 2 cut(s) 263, 281
FatI CATG 6 cut(s) 705, 766, 945, 1072, 1150, 1674
FauI CCCGC 1 cut(s) 670
FauNDI CATATG 1 cut(s) 898
FbaI TGATCA 1 cut(s) 702
FblI GTMKAC 2 cut(s) 117, 1494
FokI GGATG 4 cut(s) 437, 461, 704, 1123
FriOI GRGCYC 1 cut(s) 999
FspBI CTAG 3 cut(s) 720, 1233, 1295
GsuI CTGGAG 1 cut(s) 47
HaeIII GGCC 3 cut(s) 134, 189, 1697
HapII CCGG 1 cut(s) 223
Hin1II CATG 6 cut(s) 709, 770, 949, 1076, 1154, 1678
HincII GTYRAC 1 cut(s) 910
HindII GTYRAC 1 cut(s) 910
HinfI GANTC 3 cut(s) 59, 847, 955
HpaII CCGG 1 cut(s) 223
HphI GGTGA 2 cut(s) 697, 835
Hpy166II GTNNAC 4 cut(s) 118, 910, 1495, 1690
Hpy188I TCNGA 4 cut(s) 468, 606, 835, 1638
Hpy188III TCNNGA 8 cut(s) 165, 278, 491, 532, 593, 720, 1067, 1233
Hpy8I GTNNAC 4 cut(s) 118, 910, 1495, 1690
Hpy99I CGWCG 5 cut(s) 14, 53, 125, 128, 140
HpyCH4III ACNGT 6 cut(s) 37, 56, 613, 1032, 1568, 1600
HpyCH4IV ACGT 3 cut(s) 126, 1509, 1624
HpyF10VI GCNNNNNNNGC 5 cut(s) 1082, 1181, 1613, 1616, 1625
HpyF3I CTNAG 3 cut(s) 951, 962, 1637
HpySE526I ACGT 3 cut(s) 126, 1509, 1624
Hsp92II CATG 6 cut(s) 709, 770, 949, 1076, 1154, 1678
KpnI GGTACC 1 cut(s) 879
Ksp22I TGATCA 1 cut(s) 702
LguI GCTCTTC 2 cut(s) 89, 143
LmnI GCTCC 6 cut(s) 225, 309, 477, 577, 994, 1477
Lsp1109I GCAGC 7 cut(s) 165, 240, 1094, 1600, 1619, 1622, 1628
LweI GCATC 3 cut(s) 618, 748, 1642
MaeI CTAG 3 cut(s) 720, 1233, 1295
MaeII ACGT 3 cut(s) 126, 1509, 1624
MaeIII GTNAC 2 cut(s) 56, 1141
MhlI GDGCHC 2 cut(s) 180, 999
MluCI AATT 6 cut(s) 527, 662, 715, 749, 1055, 1342
MlyI GAGTC 2 cut(s) 53, 949
MmeI TCCRAC 1 cut(s) 1086
Mph1103I ATGCAT 2 cut(s) 768, 1635
MseI TTAA 4 cut(s) 555, 942, 1220, 1341
MslI CAYNNNNRTG 4 cut(s) 183, 1030, 1551, 1553
MspA1I CMGCKG 2 cut(s) 231, 1613
MspI CCGG 1 cut(s) 223
MspR9I CCNGG 2 cut(s) 26, 1305
MvaI CCWGG 2 cut(s) 26, 1305
MvnI CGCG 4 cut(s) 192, 273, 285, 1539
MwoI GCNNNNNNNGC 5 cut(s) 1082, 1181, 1613, 1616, 1625
NdeI CATATG 1 cut(s) 898
NlaIII CATG 6 cut(s) 709, 770, 949, 1076, 1154, 1678
NlaIV GGNNCC 6 cut(s) 218, 269, 479, 579, 877, 1479
NmuCI GTSAC 1 cut(s) 56
NsiI ATGCAT 2 cut(s) 768, 1635
NspI RCATGY 2 cut(s) 770, 1076
PciSI GCTCTTC 2 cut(s) 89, 143
PcsI WCGNNNNNNNCGW 2 cut(s) 15, 132
PfeI GAWTC 1 cut(s) 847
Ple19I CGATCG 2 cut(s) 277, 728
PleI GAGTC 2 cut(s) 53, 949
PpsI GAGTC 2 cut(s) 53, 949
Psp124BI GAGCTC 1 cut(s) 999
Psp6I CCWGG 2 cut(s) 24, 1303
PspGI CCWGG 2 cut(s) 24, 1303
PspN4I GGNNCC 6 cut(s) 218, 269, 479, 579, 877, 1479
PspPI GGNCC 5 cut(s) 162, 268, 893, 986, 1258
PsrI GAACNNNNNNTAC 2 cut(s) 1273, 1305
PstI CTGCAG 2 cut(s) 1405, 1618
PstNI CAGNNNCTG 2 cut(s) 1271, 1613
PvuI CGATCG 2 cut(s) 277, 728
PvuII CAGCTG 1 cut(s) 1613
RsaI GTAC 4 cut(s) 17, 696, 877, 1302
RsaNI GTAC 4 cut(s) 16, 695, 876, 1301
RseI CAYNNNNRTG 4 cut(s) 183, 1030, 1551, 1553
SacI GAGCTC 1 cut(s) 999
SapI GCTCTTC 2 cut(s) 89, 143
SaqAI TTAA 4 cut(s) 555, 942, 1220, 1341
Sau96I GGNCC 5 cut(s) 162, 268, 893, 986, 1258
SchI GAGTC 2 cut(s) 53, 949
ScrFI CCNGG 2 cut(s) 26, 1305
SduI GDGCHC 2 cut(s) 180, 999
SfaNI GCATC 3 cut(s) 618, 748, 1642
SfcI CTRYAG 4 cut(s) 100, 780, 1401, 1614
SinI GGWCC 5 cut(s) 162, 268, 893, 986, 1258
SmiMI CAYNNNNRTG 4 cut(s) 183, 1030, 1551, 1553
SmlI CTYRAG 1 cut(s) 1683
SmoI CTYRAG 1 cut(s) 1683
Sse9I AATT 6 cut(s) 527, 662, 715, 749, 1055, 1342
SsiI CCGC 9 cut(s) 171, 190, 231, 271, 285, 288, 677, 1261, 1588
SspMI CTAG 3 cut(s) 720, 1233, 1295
SstI GAGCTC 1 cut(s) 999
StyD4I CCNGG 2 cut(s) 24, 1303
TaaI ACNGT 6 cut(s) 37, 56, 613, 1032, 1568, 1600
TaiI ACGT 3 cut(s) 129, 1512, 1627
TaqI TCGA 5 cut(s) 9, 62, 531, 728, 1120
TaqII GACCGA 2 cut(s) 309, 659
TasI AATT 6 cut(s) 527, 662, 715, 749, 1055, 1342
TauI GCSGC 5 cut(s) 174, 192, 234, 288, 291
TfiI GAWTC 1 cut(s) 847
Tru1I TTAA 4 cut(s) 555, 942, 1220, 1341
Tru9I TTAA 4 cut(s) 555, 942, 1220, 1341
TscAI CASTG 7 cut(s) 241, 490, 639, 996, 1037, 1435, 1573
TseFI GTSAC 1 cut(s) 56
TseI GCWGC 7 cut(s) 153, 228, 1082, 1607, 1610, 1613, 1616
Tsp45I GTSAC 1 cut(s) 56
TspDTI ATGAA 6 cut(s) 551, 675, 1125, 1367, 1533, 1663
TspGWI ACGGA 3 cut(s) 1129, 1484, 1490
TspRI CASTG 7 cut(s) 241, 490, 639, 996, 1037, 1435, 1573
VpaK11BI GGWCC 5 cut(s) 162, 268, 893, 986, 1258
XapI RAATTY 4 cut(s) 527, 662, 715, 749
XbaI TCTAGA 2 cut(s) 719, 1232
XceI RCATGY 2 cut(s) 770, 1076
XmiI GTMKAC 2 cut(s) 117, 1494
XspI CTAG 3 cut(s) 720, 1233, 1295
Zsp2I ATGCAT 2 cut(s) 768, 1635
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.