AT2G20815

QWRF motif-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0010969
Physical Location & Seq
Forward (+)
8959255 .. 8962149
2895 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G20815.2

Sequence Viewer

Length: 1449 bp
ATGAAGAGCTGTGAGCATGAACTACTCAAAACAAGAAGAGGAAAGTCCCGCGAGGTTAGTTCACGTTTTCTCTCTTCGCCGTCTGCATCTTCTTCTCCTAACCGCAGAAACTCCACTTCTAATTCATCGAGAGATGATCAGAACAACAACGGGGTAAAAGGTCATCTAGGCTTGAAAAAGCATGATAGGATGTCAGATGGCACACGGGTTTGTTTCGGGTTACCAAACCAAAGTAGCATAGAAGTTGACACAAAAGAAAATCGTATGCCTTCACCGTGGATCAATGATGAGGATAACGTCATACTTCCTGGCAGATTTTCTGTTGATGAATGTGCATTGTACAGAGCATCATCAAGGAGAAACTCATGCTCTCTGCTATATGAGTCCTTCAACGATGAAACAGACTCAGAGTTGAGTGATGTGTCCTGTGCTTCAAGTCTTAGTACAAATCGTTCATCCTGGAACCATAAACCCGGTATCAAGGTCTCTTCTAAGTATCTGCATGATTTAACAGCTAAACCGAGCAAAGGCAACAACAAGACCAAACTAAGGTCACAAGATGATTCACAGAGAACTAATAGCTCTAAGGGCATTGAGAATAGATTGCAGAGGAATAATTCAGTGTCCAGGTATGGAAGTTCGATGTCTCAGTGGGCTCTTTCACCAGGAAGGTCATTGGACACTCAGGCGGTGACAGTTCCAAGTTCAAAACTGAAGCCTCCAAGAGGAAAAGGCGTGGGTAAACTAATCAACCTTGGTTTCGATTTCTTCAGGAGCAAGAACAAGTCATCGCCCTTTACTTCACCACTAAAACCAAAGACATGTGACACAGAATCTGCTCATCAGCTGAAGCTTATGAATAACCGGTTGCTTCAATGGAGGTTTGTCAATGCTAGAGCCTGTGATGTAAACAAGAACGTAGCCTCCCAAGAAAAGAATCAACTACTCTGTGCATGGGATACTCTTATCAAGTTAAATAATTTGGTTCTGCAAGAGAGAATCAAACTGCAGAAGAAAAACCTGGAGATGAAGCTGAATTATGTGTTTCTTTCTCAAGTTAAGCATCTTGAAGCTTGGGAGGACATGGAAATACAACACCTTTCATCTCTGTCCATAATCAGAGATTCCCTGCATTCTGTTCTTTCCAGGCTTCCCCTCAAAGAAGGCGCGAAGGTGAACTTGGAATCAGCTGTCAGTATAATTAAGAATGCAGAAGCAGTCACTGATGCTATCATTTCAACTGTGGATGATTATGCTCCCACGATGGAGGGTATTGTTCCACTTGCTTCCCAACTAGCGGAAGTGGTTGTACAAGAGAAGTTGATGCTCGAGAAATGCCATGACCTTTTGAGAATGATTTCCGAGTTAGAGATGCAGGAGAGGAGCTTGAAATGCTGCTTCCTGATTCAACACAAACAGACTTTCGATACAAATCTTCTTAAACACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
QWRF

Protein Analysis

482

Amino Acids

54.3

Weight (kDa)

9.35

Isoelectric Point (pI)

51.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
QWRF PF04484 118 - 416 3.3e-79 QWRF family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 51, 1169
AciI CCGC 4 cut(s) 49, 103, 689, 1298
AclWI GGATC 1 cut(s) 287
AcuI CTGAAG 3 cut(s) 734, 754, 869
AfaI GTAC 3 cut(s) 341, 445, 1311
AfiI CCNNNNNNNGG 4 cut(s) 527, 549, 725, 1297
AflIII ACRYGT 1 cut(s) 821
AgeI ACCGGT 1 cut(s) 864
AgsI TTSAA 9 cut(s) 175, 391, 435, 708, 875, 1070, 1239, 1390, 1409
AjnI CCWGG 6 cut(s) 307, 458, 626, 664, 1020, 1145
AjuI GAANNNNNNNTTGG 2 cut(s) 1163, 1195
AloI GAACNNNNNNTCC 2 cut(s) 908, 940
AluBI AGCT 9 cut(s) 9, 515, 582, 847, 853, 1033, 1073, 1190, 1386
AluI AGCT 9 cut(s) 9, 515, 582, 847, 853, 1033, 1073, 1190, 1386
Alw26I GTCTC 2 cut(s) 490, 651
AlwI GGATC 1 cut(s) 287
AlwNI CAGNNNCTG 2 cut(s) 836, 1223
Ama87I CYCGRG 1 cut(s) 1328
ApeKI GCWGC 1 cut(s) 1395
AsiGI ACCGGT 1 cut(s) 864
Asp700I GAANNNNTTC 1 cut(s) 1357
AspLEI GCGC 1 cut(s) 1169
AsuC2I CCSGG 1 cut(s) 474
AsuHPI GGTGA 5 cut(s) 264, 654, 703, 795, 1186
AvaI CYCGRG 1 cut(s) 1328
BanII GRGCYC 1 cut(s) 658
BarI GAAGNNNNNNTAC 2 cut(s) 1293, 1325
BbvI GCAGC 1 cut(s) 1382
BccI CCATC 2 cut(s) 191, 1258
BceAI ACGGC 1 cut(s) 64
BciT130I CCWGG 6 cut(s) 309, 460, 628, 666, 1022, 1147
BciVI GTATCC 1 cut(s) 952
BclI TGATCA 1 cut(s) 136
BcnI CCSGG 1 cut(s) 474
BcoDI GTCTC 2 cut(s) 490, 651
BfaI CTAG 4 cut(s) 167, 894, 1295, 1447
BfmI CTRYAG 1 cut(s) 1007
BfuI GTATCC 1 cut(s) 952
BisI GCNGC 1 cut(s) 1396
BlsI GCNGC 1 cut(s) 1397
Bme1390I CCNGG 7 cut(s) 309, 460, 474, 628, 666, 1022, 1147
BmeT110I CYCGRG 1 cut(s) 1328
BmiI GGNNCC 1 cut(s) 464
BmrFI CCNGG 7 cut(s) 309, 460, 474, 628, 666, 1022, 1147
BmsI GCATC 6 cut(s) 95, 356, 1072, 1216, 1314, 1362
BpmI CTGGAG 1 cut(s) 1043
BpuEI CTTGAG 1 cut(s) 1038
BpuMI CCSGG 1 cut(s) 474
BsaBI GATNNNNATC 1 cut(s) 1431
BsaI GGTCTC 1 cut(s) 490
BsaJI CCNNGG 2 cut(s) 275, 754
BsaWI WCCGGW 1 cut(s) 864
BsaXI ACNNNNNCTCC 2 cut(s) 908, 938
Bsc4I CCNNNNNNNGG 4 cut(s) 527, 549, 725, 1297
Bse118I RCCGGY 1 cut(s) 864
Bse8I GATNNNNATC 1 cut(s) 1431
BseBI CCWGG 6 cut(s) 309, 460, 628, 666, 1022, 1147
BseDI CCNNGG 2 cut(s) 275, 754
BseGI GGATG 3 cut(s) 195, 455, 1252
BseJI GATNNNNATC 1 cut(s) 1431
BseLI CCNNNNNNNGG 4 cut(s) 527, 549, 725, 1297
BseMII CTCAG 3 cut(s) 420, 662, 698
BseRI GAGGAG 1 cut(s) 1396
BseXI GCAGC 1 cut(s) 1382
Bsh1236I CGCG 2 cut(s) 51, 1169
BshTI ACCGGT 1 cut(s) 864
BsiHKCI CYCGRG 1 cut(s) 1328
BsiSI CCGG 2 cut(s) 474, 865
BslFI GGGAC 1 cut(s) 31
BslI CCNNNNNNNGG 4 cut(s) 527, 549, 725, 1297
BsmAI GTCTC 2 cut(s) 490, 651
BsmFI GGGAC 1 cut(s) 31
BsmI GAATGC 2 cut(s) 1132, 1213
Bso31I GGTCTC 1 cut(s) 490
BsoBI CYCGRG 1 cut(s) 1328
Bsp1286I GDGCHC 1 cut(s) 658
Bsp1407I TGTACA 2 cut(s) 339, 1309
Bsp143I GATC 2 cut(s) 136, 279
BspACI CCGC 4 cut(s) 49, 103, 689, 1298
BspCNI CTCAG 3 cut(s) 419, 661, 697
BspFNI CGCG 2 cut(s) 51, 1169
BspLI GGNNCC 1 cut(s) 464
BspMAI CTGCAG 1 cut(s) 1011
BspPI GGATC 1 cut(s) 287
BspTNI GGTCTC 1 cut(s) 490
BsrFI RCCGGY 1 cut(s) 864
BsrGI TGTACA 2 cut(s) 339, 1309
BssAI RCCGGY 1 cut(s) 864
BssECI CCNNGG 2 cut(s) 275, 754
BssMI GATC 2 cut(s) 136, 279
BssT1I CCWWGG 1 cut(s) 754
Bst2UI CCWGG 6 cut(s) 309, 460, 628, 666, 1022, 1147
Bst4CI ACNGT 3 cut(s) 276, 697, 1243
Bst6I CTCTTC 3 cut(s) 31, 79, 493
BstAUI TGTACA 2 cut(s) 339, 1309
BstDEI CTNAG 7 cut(s) 406, 440, 492, 548, 585, 648, 684
BstDSI CCRYGG 1 cut(s) 275
BstEII GGTNACC 1 cut(s) 219
BstENI CCTNNNNNAGG 1 cut(s) 723
BstF5I GGATG 3 cut(s) 195, 455, 1252
BstFNI CGCG 2 cut(s) 51, 1169
BstHHI GCGC 1 cut(s) 1169
BstKTI GATC 2 cut(s) 139, 282
BstMAI GTCTC 2 cut(s) 490, 651
BstMBI GATC 2 cut(s) 136, 279
BstMWI GCNNNNNNNGC 2 cut(s) 588, 1392
BstNI CCWGG 6 cut(s) 309, 460, 628, 666, 1022, 1147
BstNSI RCATGY 1 cut(s) 825
BstPI GGTNACC 1 cut(s) 219
BstSCI CCNGG 7 cut(s) 307, 458, 472, 626, 664, 1020, 1145
BstSFI CTRYAG 1 cut(s) 1007
BstUI CGCG 2 cut(s) 51, 1169
BstV1I GCAGC 1 cut(s) 1382
BsuI GTATCC 1 cut(s) 952
BtgI CCRYGG 1 cut(s) 275
BtgZI GCGATG 1 cut(s) 774
BtsCI GGATG 3 cut(s) 195, 455, 1252
BtsIMutI CAGTG 3 cut(s) 627, 656, 1221
CaiI CAGNNNCTG 2 cut(s) 836, 1223
CfoI GCGC 1 cut(s) 1169
Cfr10I RCCGGY 1 cut(s) 864
Csp6I GTAC 3 cut(s) 340, 444, 1310
CspAI ACCGGT 1 cut(s) 864
CviAII CATG 8 cut(s) 17, 182, 366, 503, 822, 954, 1084, 1340
CviQI GTAC 3 cut(s) 340, 444, 1310
DdeI CTNAG 7 cut(s) 406, 440, 492, 548, 585, 648, 684
DpnI GATC 2 cut(s) 138, 281
DpnII GATC 2 cut(s) 136, 279
Eam1104I CTCTTC 3 cut(s) 31, 79, 493
EarI CTCTTC 3 cut(s) 31, 79, 493
Eco130I CCWWGG 1 cut(s) 754
Eco24I GRGCYC 1 cut(s) 658
Eco31I GGTCTC 1 cut(s) 490
Eco57I CTGAAG 3 cut(s) 734, 754, 869
Eco88I CYCGRG 1 cut(s) 1328
Eco91I GGTNACC 1 cut(s) 219
EcoNI CCTNNNNNAGG 1 cut(s) 723
EcoO65I GGTNACC 1 cut(s) 219
EcoRII CCWGG 6 cut(s) 307, 458, 626, 664, 1020, 1145
EcoT14I CCWWGG 1 cut(s) 754
EcoT38I GRGCYC 1 cut(s) 658
ErhI CCWWGG 1 cut(s) 754
FaeI CATG 8 cut(s) 20, 185, 369, 506, 825, 957, 1087, 1343
FalI AAGNNNNNCTT 2 cut(s) 1163, 1195
FaqI GGGAC 1 cut(s) 31
FatI CATG 8 cut(s) 16, 181, 365, 502, 821, 953, 1083, 1339
FauI CCCGC 1 cut(s) 56
FbaI TGATCA 1 cut(s) 136
Fnu4HI GCNGC 1 cut(s) 1396
FokI GGATG 3 cut(s) 202, 442, 1259
FriOI GRGCYC 1 cut(s) 658
Fsp4HI GCNGC 1 cut(s) 1396
FspBI CTAG 4 cut(s) 167, 894, 1295, 1447
GlaI GCGC 1 cut(s) 1168
GluI GCNGC 1 cut(s) 1396
GsuI CTGGAG 1 cut(s) 1043
HapII CCGG 2 cut(s) 474, 865
HhaI GCGC 1 cut(s) 1169
Hin1II CATG 8 cut(s) 20, 185, 369, 506, 825, 957, 1087, 1343
Hin6I GCGC 1 cut(s) 1167
HinP1I GCGC 1 cut(s) 1167
HincII GTYRAC 1 cut(s) 247
HindII GTYRAC 1 cut(s) 247
HindIII AAGCTT 2 cut(s) 851, 1071
HinfI GANTC 9 cut(s) 383, 404, 563, 833, 937, 999, 1124, 1184, 1405
HpaII CCGG 2 cut(s) 474, 865
HphI GGTGA 5 cut(s) 264, 654, 703, 795, 1186
Hpy166II GTNNAC 5 cut(s) 62, 247, 743, 910, 1177
Hpy188I TCNGA 5 cut(s) 141, 196, 409, 1121, 1363
Hpy188III TCNNGA 5 cut(s) 129, 772, 1067, 1330, 1402
Hpy8I GTNNAC 5 cut(s) 62, 247, 743, 910, 1177
HpyAV CCTTC 5 cut(s) 279, 397, 663, 1157, 1165
HpyCH4III ACNGT 3 cut(s) 276, 697, 1243
HpyCH4IV ACGT 3 cut(s) 64, 297, 918
HpyF10VI GCNNNNNNNGC 2 cut(s) 588, 1392
HpyF3I CTNAG 7 cut(s) 406, 440, 492, 548, 585, 648, 684
HpySE526I ACGT 3 cut(s) 64, 297, 918
Hsp92II CATG 8 cut(s) 20, 185, 369, 506, 825, 957, 1087, 1343
HspAI GCGC 1 cut(s) 1167
Ksp22I TGATCA 1 cut(s) 136
Kzo9I GATC 2 cut(s) 136, 279
LmnI GCTCC 3 cut(s) 774, 1261, 1383
Lsp1109I GCAGC 1 cut(s) 1382
LweI GCATC 6 cut(s) 95, 356, 1072, 1216, 1314, 1362
MaeI CTAG 4 cut(s) 167, 894, 1295, 1447
MaeII ACGT 3 cut(s) 64, 297, 918
MaeIII GTNAC 5 cut(s) 219, 552, 691, 824, 1219
MalI GATC 2 cut(s) 138, 281
MboI GATC 2 cut(s) 136, 279
MboII GAAGA 9 cut(s) 16, 48, 66, 81, 84, 480, 760, 1024, 1427
MhlI GDGCHC 1 cut(s) 658
MluCI AATT 5 cut(s) 121, 616, 979, 1036, 1200
MlyI GAGTC 2 cut(s) 392, 398
MroXI GAANNNNTTC 1 cut(s) 1357
MseI TTAA 5 cut(s) 509, 974, 1059, 1203, 1440
MspA1I CMGCKG 2 cut(s) 847, 1190
MspI CCGG 2 cut(s) 474, 865
MspR9I CCNGG 7 cut(s) 309, 460, 474, 628, 666, 1022, 1147
Mva1269I GAATGC 2 cut(s) 1132, 1213
MvaI CCWGG 6 cut(s) 309, 460, 628, 666, 1022, 1147
MvnI CGCG 2 cut(s) 51, 1169
MwoI GCNNNNNNNGC 2 cut(s) 588, 1392
NciI CCSGG 1 cut(s) 474
NdeII GATC 2 cut(s) 136, 279
NlaIII CATG 8 cut(s) 20, 185, 369, 506, 825, 957, 1087, 1343
NlaIV GGNNCC 1 cut(s) 464
NmuCI GTSAC 4 cut(s) 552, 691, 824, 1219
NspI RCATGY 1 cut(s) 825
PaeR7I CTCGAG 1 cut(s) 1328
PciI ACATGT 1 cut(s) 821
PctI GAATGC 2 cut(s) 1132, 1213
PdmI GAANNNNTTC 1 cut(s) 1357
PfeI GAWTC 7 cut(s) 563, 833, 937, 999, 1124, 1184, 1405
PfoI TCCNGGA 1 cut(s) 458
PinAI ACCGGT 1 cut(s) 864
PkrI GCNGC 1 cut(s) 1397
PleI GAGTC 2 cut(s) 391, 398
PpsI GAGTC 2 cut(s) 391, 398
PscI ACATGT 1 cut(s) 821
Psp6I CCWGG 6 cut(s) 307, 458, 626, 664, 1020, 1145
PspEI GGTNACC 1 cut(s) 219
PspGI CCWGG 6 cut(s) 307, 458, 626, 664, 1020, 1145
PspN4I GGNNCC 1 cut(s) 464
PsrI GAACNNNNNNTAC 2 cut(s) 436, 468
PstI CTGCAG 1 cut(s) 1011
PstNI CAGNNNCTG 2 cut(s) 836, 1223
PvuII CAGCTG 2 cut(s) 847, 1190
RsaI GTAC 3 cut(s) 341, 445, 1311
RsaNI GTAC 3 cut(s) 340, 444, 1310
SaqAI TTAA 5 cut(s) 509, 974, 1059, 1203, 1440
SatI GCNGC 1 cut(s) 1396
Sau3AI GATC 2 cut(s) 136, 279
SchI GAGTC 2 cut(s) 392, 398
ScrFI CCNGG 7 cut(s) 309, 460, 474, 628, 666, 1022, 1147
SduI GDGCHC 1 cut(s) 658
SfaNI GCATC 6 cut(s) 95, 356, 1072, 1216, 1314, 1362
SfcI CTRYAG 1 cut(s) 1007
Sfr274I CTCGAG 1 cut(s) 1328
SlaI CTCGAG 1 cut(s) 1328
SmlI CTYRAG 2 cut(s) 1053, 1328
SmoI CTYRAG 2 cut(s) 1053, 1328
Sse9I AATT 5 cut(s) 121, 616, 979, 1036, 1200
SsiI CCGC 4 cut(s) 49, 103, 689, 1298
SspMI CTAG 4 cut(s) 167, 894, 1295, 1447
StyD4I CCNGG 7 cut(s) 307, 458, 472, 626, 664, 1020, 1145
StyI CCWWGG 1 cut(s) 754
TaaI ACNGT 3 cut(s) 276, 697, 1243
TaiI ACGT 3 cut(s) 67, 300, 921
TaqI TCGA 5 cut(s) 128, 641, 762, 1329, 1425
TasI AATT 5 cut(s) 121, 616, 979, 1036, 1200
TatI WGTACW 3 cut(s) 339, 443, 1309
TfiI GAWTC 7 cut(s) 563, 833, 937, 999, 1124, 1184, 1405
Tru1I TTAA 5 cut(s) 509, 974, 1059, 1203, 1440
Tru9I TTAA 5 cut(s) 509, 974, 1059, 1203, 1440
TscAI CASTG 3 cut(s) 627, 656, 1228
TseFI GTSAC 4 cut(s) 552, 691, 824, 1219
TseI GCWGC 1 cut(s) 1395
Tsp45I GTSAC 4 cut(s) 552, 691, 824, 1219
TspDTI ATGAA 9 cut(s) 17, 33, 114, 342, 411, 444, 872, 1043, 1092
TspRI CASTG 3 cut(s) 627, 656, 1228
XagI CCTNNNNNAGG 1 cut(s) 723
XceI RCATGY 1 cut(s) 825
XhoI CTCGAG 1 cut(s) 1328
XmnI GAANNNNTTC 1 cut(s) 1357
XspI CTAG 4 cut(s) 167, 894, 1295, 1447
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.