Rorug02G0493800

QWRF motif-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0010969
Physical Location & Seq
Forward (+)
62277399 .. 62277983
585 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0493800.1

Sequence Viewer

Length: 585 bp
ATGCTAATAGAAGGGGTGAATCCAGATCAACTGACCTTTGTTGGGGTGTTGTCTGCATGCAGTCACAGTGGGCTGGTTGATTGGGGATTAAAATTGTTTAAGAGCATGACTCGAGTTAACCTTATTGAACCTCTAGCTGAACACTATGCTTGCATGGTAGACTTGCTTGGCCGTGCAGGGAGGTTAGAGGAAGCCTTTGAAATGGTGAGGGATATGAAGGTCAAGGCAACTGCAAGAATGTGGGGTGCATTACTTGGAGCTTCCAGGATACACCGGAATCTAAAGTTTGCCAATTATGCTACCAAGAAACTTTTAGAACTCGAACCTGATAAAACTTCAAATTTTGTGCTATTGTCAAACATGAATGCTGAGGTAGGCAGATGGGATGAGGTTGAAATAGTCAGCATGTTGATTAACCAACGTAAAATGGAGAAGCAACCCGGCCGCAGTTGGATTGAAGTCAGGAATCATGCTTTTCTCTTTGATGATCCAGTGCAACCCAGAACAGCAGAGATATGCAGTGTACTGAACTCCCTAGCCAGAGAGATGAAAAACACTAGTTCCTTCATGACATCTTACAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
QWRF

Protein Analysis

194

Amino Acids

22.04

Weight (kDa)

7.74

Isoelectric Point (pI)

37.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
E_motif PF20431 91 - 153 6.5e-17 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 159
AciI CCGC 1 cut(s) 445
AclWI GGATC 1 cut(s) 482
AcoI YGGCCR 2 cut(s) 169, 442
AcsI RAATTY 1 cut(s) 340
AfaI GTAC 1 cut(s) 525
AfiI CCNNNNNNNGG 1 cut(s) 42
AgsI TTSAA 5 cut(s) 128, 200, 339, 395, 458
AhlI ACTAGT 1 cut(s) 557
AjnI CCWGG 1 cut(s) 263
AluBI AGCT 2 cut(s) 137, 260
AluI AGCT 2 cut(s) 137, 260
AlwI GGATC 1 cut(s) 482
Ama87I CYCGRG 1 cut(s) 111
AoxI GGCC 2 cut(s) 169, 442
ApoI RAATTY 1 cut(s) 340
AsuC2I CCSGG 1 cut(s) 441
AsuHPI GGTGA 2 cut(s) 28, 217
AvaI CYCGRG 1 cut(s) 111
BbvCI CCTCAGC 1 cut(s) 369
BccI CCATC 1 cut(s) 375
BceAI ACGGC 1 cut(s) 156
BciT130I CCWGG 1 cut(s) 265
BciVI GTATCC 1 cut(s) 261
BcnI CCSGG 1 cut(s) 441
BcuI ACTAGT 1 cut(s) 557
BfaI CTAG 3 cut(s) 134, 536, 558
BfuI GTATCC 1 cut(s) 261
BisI GCNGC 1 cut(s) 445
BlsI GCNGC 1 cut(s) 446
Bme1390I CCNGG 2 cut(s) 265, 441
BmeT110I CYCGRG 1 cut(s) 111
BmrFI CCNGG 2 cut(s) 265, 441
BplI GAGNNNNNCTC 2 cut(s) 94, 126
Bpu10I CCTNAGC 1 cut(s) 369
BpuMI CCSGG 1 cut(s) 441
BsaWI WCCGGW 1 cut(s) 273
Bsc4I CCNNNNNNNGG 1 cut(s) 42
Bse1I ACTGG 1 cut(s) 491
BseBI CCWGG 1 cut(s) 265
BseGI GGATG 1 cut(s) 391
BseLI CCNNNNNNNGG 1 cut(s) 42
BseMII CTCAG 1 cut(s) 360
BseNI ACTGG 1 cut(s) 491
BseX3I CGGCCG 1 cut(s) 442
BsgI GTGCAG 1 cut(s) 195
Bsh1285I CGRYCG 1 cut(s) 445
BshFI GGCC 2 cut(s) 171, 444
BsiEI CGRYCG 1 cut(s) 445
BsiHKCI CYCGRG 1 cut(s) 111
BsiSI CCGG 2 cut(s) 274, 441
BslI CCNNNNNNNGG 1 cut(s) 42
BsmI GAATGC 1 cut(s) 370
BsnI GGCC 2 cut(s) 171, 444
BsoBI CYCGRG 1 cut(s) 111
Bsp143I GATC 2 cut(s) 25, 487
BspACI CCGC 1 cut(s) 445
BspANI GGCC 2 cut(s) 171, 444
BspCNI CTCAG 1 cut(s) 361
BspHI TCATGA 1 cut(s) 567
BspPI GGATC 1 cut(s) 482
BsrI ACTGG 1 cut(s) 491
BssMI GATC 2 cut(s) 25, 487
Bst2UI CCWGG 1 cut(s) 265
Bst4CI ACNGT 1 cut(s) 68
BstC8I GCNNGC 2 cut(s) 58, 151
BstDEI CTNAG 1 cut(s) 369
BstF5I GGATG 1 cut(s) 391
BstKTI GATC 2 cut(s) 28, 490
BstMBI GATC 2 cut(s) 25, 487
BstMCI CGRYCG 1 cut(s) 445
BstMWI GCNNNNNNNGC 1 cut(s) 296
BstNI CCWGG 1 cut(s) 265
BstNSI RCATGY 2 cut(s) 60, 409
BstSCI CCNGG 2 cut(s) 263, 439
BstZI CGGCCG 1 cut(s) 442
BsuI GTATCC 1 cut(s) 261
BsuRI GGCC 2 cut(s) 171, 444
BtsCI GGATG 1 cut(s) 391
BtsI GCAGTG 1 cut(s) 526
BtsIMutI CAGTG 3 cut(s) 73, 498, 526
Cac8I GCNNGC 2 cut(s) 58, 151
CciI TCATGA 1 cut(s) 567
Csp6I GTAC 1 cut(s) 524
CviAII CATG 7 cut(s) 57, 106, 154, 361, 406, 470, 568
CviJI RGCY 7 cut(s) 73, 137, 171, 194, 260, 444, 539
CviKI_1 RGCY 7 cut(s) 73, 137, 171, 194, 260, 444, 539
CviQI GTAC 1 cut(s) 524
DdeI CTNAG 1 cut(s) 369
DpnI GATC 2 cut(s) 27, 489
DpnII GATC 2 cut(s) 25, 487
EaeI YGGCCR 2 cut(s) 169, 442
EagI CGGCCG 1 cut(s) 442
EclXI CGGCCG 1 cut(s) 442
Eco52I CGGCCG 1 cut(s) 442
Eco88I CYCGRG 1 cut(s) 111
EcoRII CCWGG 1 cut(s) 263
FaeI CATG 7 cut(s) 60, 109, 157, 364, 409, 473, 571
FatI CATG 7 cut(s) 56, 105, 153, 360, 405, 469, 567
FblI GTMKAC 1 cut(s) 159
Fnu4HI GCNGC 1 cut(s) 445
FokI GGATG 1 cut(s) 398
Fsp4HI GCNGC 1 cut(s) 445
FspBI CTAG 3 cut(s) 134, 536, 558
GluI GCNGC 1 cut(s) 445
HaeIII GGCC 2 cut(s) 171, 444
HapII CCGG 2 cut(s) 274, 441
Hin1II CATG 7 cut(s) 60, 109, 157, 364, 409, 473, 571
HincII GTYRAC 1 cut(s) 118
HindII GTYRAC 1 cut(s) 118
HinfI GANTC 4 cut(s) 19, 109, 277, 466
HpaI GTTAAC 1 cut(s) 118
HpaII CCGG 2 cut(s) 274, 441
HphI GGTGA 2 cut(s) 28, 217
Hpy166II GTNNAC 3 cut(s) 118, 160, 524
Hpy188III TCNNGA 3 cut(s) 23, 463, 568
Hpy8I GTNNAC 3 cut(s) 118, 160, 524
HpyAV CCTTC 3 cut(s) 5, 211, 574
HpyCH4III ACNGT 1 cut(s) 68
HpyCH4IV ACGT 1 cut(s) 421
HpyCH4V TGCA 8 cut(s) 56, 60, 153, 176, 233, 248, 496, 519
HpyF10VI GCNNNNNNNGC 1 cut(s) 296
HpyF3I CTNAG 1 cut(s) 369
HpySE526I ACGT 1 cut(s) 421
Hsp92II CATG 7 cut(s) 60, 109, 157, 364, 409, 473, 571
KspAI GTTAAC 1 cut(s) 118
Kzo9I GATC 2 cut(s) 25, 487
LmnI GCTCC 1 cut(s) 257
MaeI CTAG 3 cut(s) 134, 536, 558
MaeII ACGT 1 cut(s) 421
MaeIII GTNAC 1 cut(s) 62
MalI GATC 2 cut(s) 27, 489
MboI GATC 2 cut(s) 25, 487
MluCI AATT 3 cut(s) 92, 292, 340
MlyI GAGTC 1 cut(s) 103
MmeI TCCRAC 1 cut(s) 431
MnlI CCTC 6 cut(s) 141, 174, 181, 201, 364, 382
MseI TTAA 4 cut(s) 89, 99, 117, 414
MspI CCGG 2 cut(s) 274, 441
MspR9I CCNGG 2 cut(s) 265, 441
Mva1269I GAATGC 1 cut(s) 370
MvaI CCWGG 1 cut(s) 265
MwoI GCNNNNNNNGC 1 cut(s) 296
NciI CCSGG 1 cut(s) 441
NdeII GATC 2 cut(s) 25, 487
NlaIII CATG 7 cut(s) 60, 109, 157, 364, 409, 473, 571
NmuCI GTSAC 1 cut(s) 62
NspI RCATGY 2 cut(s) 60, 409
PaeI GCATGC 1 cut(s) 60
PaeR7I CTCGAG 1 cut(s) 111
PagI TCATGA 1 cut(s) 567
PctI GAATGC 1 cut(s) 370
PfeI GAWTC 3 cut(s) 19, 277, 466
PfoI TCCNGGA 1 cut(s) 263
PkrI GCNGC 1 cut(s) 446
PleI GAGTC 1 cut(s) 103
PpsI GAGTC 1 cut(s) 103
Psp6I CCWGG 1 cut(s) 263
PspGI CCWGG 1 cut(s) 263
PspXI VCTCGAGB 1 cut(s) 111
RsaI GTAC 1 cut(s) 525
RsaNI GTAC 1 cut(s) 524
SaqAI TTAA 4 cut(s) 89, 99, 117, 414
SatI GCNGC 1 cut(s) 445
Sau3AI GATC 2 cut(s) 25, 487
SchI GAGTC 1 cut(s) 103
ScrFI CCNGG 2 cut(s) 265, 441
Sfr274I CTCGAG 1 cut(s) 111
SlaI CTCGAG 1 cut(s) 111
SmlI CTYRAG 1 cut(s) 111
SmoI CTYRAG 1 cut(s) 111
SpeI ACTAGT 1 cut(s) 557
SphI GCATGC 1 cut(s) 60
Sse9I AATT 3 cut(s) 92, 292, 340
SsiI CCGC 1 cut(s) 445
SspMI CTAG 3 cut(s) 134, 536, 558
StyD4I CCNGG 2 cut(s) 263, 439
TaaI ACNGT 1 cut(s) 68
TaiI ACGT 1 cut(s) 424
TaqI TCGA 2 cut(s) 112, 321
TasI AATT 3 cut(s) 92, 292, 340
TatI WGTACW 1 cut(s) 523
TauI GCSGC 1 cut(s) 447
TfiI GAWTC 3 cut(s) 19, 277, 466
Tru1I TTAA 4 cut(s) 89, 99, 117, 414
Tru9I TTAA 4 cut(s) 89, 99, 117, 414
TscAI CASTG 3 cut(s) 73, 498, 526
TseFI GTSAC 1 cut(s) 62
Tsp45I GTSAC 1 cut(s) 62
TspDTI ATGAA 4 cut(s) 230, 377, 556, 563
TspRI CASTG 3 cut(s) 73, 498, 526
XapI RAATTY 1 cut(s) 340
XceI RCATGY 2 cut(s) 60, 409
XhoI CTCGAG 1 cut(s) 111
XmiI GTMKAC 1 cut(s) 159
XspI CTAG 3 cut(s) 134, 536, 558
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.