MD17G1083000.v1.1

QWRF motif-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0010969
Physical Location & Seq
Reverse (-)
6909539 .. 6911561
2023 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1083000.v1.1.491

Sequence Viewer

Length: 1650 bp
ATGAAGAACGATCAAGTGGTGACAGACCAATCCCACAAACCCAGAAGAGCCAAATCCCGCGAAGTAAGCTCGCGGTTTCTATCATCGCCAACCACCACAGAAACCACCACGCTCCCGTCGCCGAACCATCCCATCTCGCCGGTACGGCGCAAACCTGTTTCACCTTTCTCCGATGCCCGAAAGCCCAAAACGCAGCTCGACGATTCGGGCCCCACCCGCGGACTATGGCCCTCTTCAAACTCGTCGCACAAGAAGCTCGACACTCTCGCCGACCAGCTCGGAAACGACAGGCGCGAAGATCGCGTCCAACGTAAGGATTCAATCTTTGACAGAGGAAGGAGCTGCAGAGAGTTTGGTTCGTTGGATAATCCCAAAGAGAAAGGGAGAGAAAGCGCCAAAGAACGTCACATACCGTTTCTTGGCGGGTCGATGAGATACACTGGAAAGTTCAGGTTTTCCAGAAATTCTAACTCTTCTTCTTCTTCTACTTCGTCGTCTTCCAGCAAGTTTTCAAGCAGTTCGAATGTGGCAGCTCCTCGGAGATTTTCAGTGGACGAAAATGCTCTGTATCAGAAACAATCTCGGAGAAATTCGGATGGTTTCCCGGATACCCTTGATTCGGAATCAGAGTGCAGTGACGGCTCTTCAGGGATGATTATGGGGTCTCCGAATGTTGGTTCAGCTGCTCGGAAATCGGGTGTAGAGGTTTCTTCCAAGTACATGAATGAGATACCTTTGAGGCATCGAAGAGGGACGTCGGATTCTAACATCGCAAATCCGATATCGGGAGATAAGTCTCCGAAGCCGAACAAATTCACCATTAAAAATGTGATGAGGAGGGCTCATTCTCTGACGGCGACAACACAGTGGGCGTTGTCGCCGGGAAGAACAGGGTCGCCGACGATGTCTGTGGAGAATAAGGAAGTACCAATGTCATTTTCGAGCTTGAAGCCCCCTACAAGTCCCTCGAAAACCAAAGGGGTGGAGAAGTTGTTCAACTTGGGGTTGGACTTGTTCAAGAGTAAAAAATCTTCTTCTTCTAATGTCTTGCTGGTAAGGTCTAATTCGGTTTCTTCGGGCTCAAGTATGACAGAGATGGGTCATCAACTCCGGTTGCTTCATAATCGATATTTGCAGTGGCGGTTTGCAAATGCTAGAGCTGATGTTGTGAATCAGAACATAGCAGATCAAGCACAGGTCAATGTTTTATATGCTTGGGATGCTCTGATGAAGTTACGGCATTCCGTGCTACAAAAGAAAGTGAAGTTACAAAAAGAAAGGCTTGACATGAAGCTGAACTTCATACTCTATTCTCAACTTAAGCCATTGGATTCTTGGGGAGATATGGAACGGCAACACATGGCAGCAGCTTGCATGATGAAAGAGTGCTTGCATTCTGTTGTCTGCAGGGTGCCTCTCGTTGAAGGTGCAGAGGCGGACACTCAATCTGCTTCTGTTGCTCTTCGACATGCTTCGGATCTCACCACTTCCATTAAGTTGTTGCTTTCTAATTTCTCACCATTGGCAGAGCAAGCTGCTCCGTTACTGTCGGAATTAGCGGAGGTGGTTGCACAAGAAAAATTGCTTTTAGAGGAGTGTCTTGAGCTTTTCAGAAACATTTCCACACTAGAGGTAGTAAACGGACGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
QWRF

Protein Analysis

550

Amino Acids

60.88

Weight (kDa)

9.84

Isoelectric Point (pI)

59.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
QWRF PF04484 224 - 531 4.9e-72 QWRF family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 758
AccB1I GGYRCC 1 cut(s) 1411
AccII CGCG 5 cut(s) 60, 73, 219, 294, 303
AciI CCGC 8 cut(s) 58, 73, 217, 219, 423, 1141, 1436, 1559
AclWI GGATC 1 cut(s) 1485
AcsI RAATTY 3 cut(s) 463, 589, 812
AcuI CTGAAG 1 cut(s) 630
AcyI GRCGYC 1 cut(s) 755
AfaI GTAC 3 cut(s) 144, 719, 927
AfiI CCNNNNNNNGG 6 cut(s) 218, 313, 419, 619, 674, 785
AflII CTTAAG 1 cut(s) 1319
AgsI TTSAA 7 cut(s) 237, 321, 513, 949, 997, 1018, 1424
AjuI GAANNNNNNNTTGG 2 cut(s) 922, 954
AloI GAACNNNNNNTCC 2 cut(s) 977, 1009
Alw26I GTCTC 2 cut(s) 669, 801
AlwI GGATC 1 cut(s) 1485
AoxI GGCC 2 cut(s) 208, 227
ApaI GGGCCC 1 cut(s) 212
ApeKI GCWGC 7 cut(s) 193, 342, 530, 683, 1364, 1367, 1535
ApoI RAATTY 3 cut(s) 463, 589, 812
ArsI GACNNNNNNTTYG 2 cut(s) 288, 320
Asp700I GAANNNNTTC 3 cut(s) 812, 992, 1618
AspLEI GCGC 3 cut(s) 150, 294, 395
AspS9I GGNCC 3 cut(s) 208, 209, 228
AsuC2I CCSGG 2 cut(s) 605, 882
AsuHPI GGTGA 5 cut(s) 31, 153, 808, 1474, 1509
AsuII TTCGAA 1 cut(s) 521
BaeGI GKGCMC 1 cut(s) 212
BanI GGYRCC 1 cut(s) 1411
BanII GRGCYC 3 cut(s) 212, 844, 1082
BarI GAAGNNNNNNTAC 2 cut(s) 1015, 1047
BbsI GAAGAC 1 cut(s) 489
BbvI GCAGC 7 cut(s) 205, 329, 542, 670, 1376, 1379, 1522
BccI CCATC 4 cut(s) 135, 140, 590, 1090
BceAI ACGGC 5 cut(s) 161, 655, 870, 1253, 1367
BciVI GTATCC 1 cut(s) 601
BcnI CCSGG 2 cut(s) 605, 882
BcoDI GTCTC 2 cut(s) 669, 801
BfaI CTAG 2 cut(s) 1155, 1628
BfmI CTRYAG 2 cut(s) 343, 1405
BfoI RGCGCY 1 cut(s) 396
BfrI CTTAAG 1 cut(s) 1319
BfuI GTATCC 1 cut(s) 601
BglI GCCNNNNNGGC 1 cut(s) 145
BisI GCNGC 7 cut(s) 194, 343, 531, 684, 1365, 1368, 1536
BlsI GCNGC 7 cut(s) 195, 344, 532, 685, 1366, 1369, 1537
Bme1390I CCNGG 2 cut(s) 605, 882
BmgT120I GGNCC 3 cut(s) 208, 209, 228
BmiI GGNNCC 3 cut(s) 210, 211, 1413
BmrFI CCNGG 2 cut(s) 605, 882
BmsI GCATC 3 cut(s) 163, 751, 1210
BpiI GAAGAC 1 cut(s) 489
BplI GAGNNNNNCTC 2 cut(s) 826, 858
Bpu14I TTCGAA 1 cut(s) 521
BpuEI CTTGAG 2 cut(s) 1066, 1622
BpuMI CCSGG 2 cut(s) 605, 882
Bsa29I ATCGAT 1 cut(s) 1126
BsaHI GRCGYC 1 cut(s) 755
BsaI GGTCTC 1 cut(s) 669
BsaJI CCNNGG 2 cut(s) 217, 536
BsaWI WCCGGW 1 cut(s) 1110
BsaXI ACNNNNNCTCC 2 cut(s) 977, 1007
Bsc4I CCNNNNNNNGG 6 cut(s) 218, 313, 419, 619, 674, 785
Bse118I RCCGGY 1 cut(s) 139
Bse1I ACTGG 1 cut(s) 445
BseCI ATCGAT 1 cut(s) 1126
BseDI CCNNGG 2 cut(s) 217, 536
BseGI GGATG 4 cut(s) 127, 601, 657, 1225
BseLI CCNNNNNNNGG 6 cut(s) 218, 313, 419, 619, 674, 785
BseNI ACTGG 1 cut(s) 445
BseRI GAGGAG 3 cut(s) 525, 850, 1607
BseSI GKGCMC 1 cut(s) 212
BseXI GCAGC 7 cut(s) 205, 329, 542, 670, 1376, 1379, 1522
BsgI GTGCAG 2 cut(s) 652, 1449
Bsh1236I CGCG 5 cut(s) 60, 73, 219, 294, 303
BshFI GGCC 2 cut(s) 210, 229
BshNI GGYRCC 1 cut(s) 1411
BshVI ATCGAT 1 cut(s) 1126
BsiSI CCGG 4 cut(s) 140, 605, 881, 1111
BslFI GGGAC 2 cut(s) 766, 948
BslI CCNNNNNNNGG 6 cut(s) 218, 313, 419, 619, 674, 785
BsmAI GTCTC 2 cut(s) 669, 801
BsmFI GGGAC 2 cut(s) 766, 948
BsmI GAATGC 2 cut(s) 1240, 1393
BsnI GGCC 2 cut(s) 210, 229
Bso31I GGTCTC 1 cut(s) 669
Bsp119I TTCGAA 1 cut(s) 521
Bsp120I GGGCCC 1 cut(s) 208
Bsp1286I GDGCHC 3 cut(s) 212, 844, 1082
Bsp143I GATC 4 cut(s) 10, 298, 1186, 1477
BspACI CCGC 8 cut(s) 58, 73, 217, 219, 423, 1141, 1436, 1559
BspANI GGCC 2 cut(s) 210, 229
BspDI ATCGAT 1 cut(s) 1126
BspFNI CGCG 5 cut(s) 60, 73, 219, 294, 303
BspLI GGNNCC 3 cut(s) 210, 211, 1413
BspMAI CTGCAG 2 cut(s) 347, 1409
BspPI GGATC 1 cut(s) 1485
BspQI GCTCTTC 3 cut(s) 40, 649, 1467
BspT104I TTCGAA 1 cut(s) 521
BspT107I GGYRCC 1 cut(s) 1411
BspTI CTTAAG 1 cut(s) 1319
BspTNI GGTCTC 1 cut(s) 669
BsrFI RCCGGY 1 cut(s) 139
BsrI ACTGG 1 cut(s) 445
BssAI RCCGGY 1 cut(s) 139
BssECI CCNNGG 2 cut(s) 217, 536
BssMI GATC 4 cut(s) 10, 298, 1186, 1477
BssNI GRCGYC 1 cut(s) 755
Bst4CI ACNGT 3 cut(s) 414, 867, 1548
Bst6I CTCTTC 6 cut(s) 40, 238, 478, 649, 742, 1467
BstACI GRCGYC 1 cut(s) 755
BstAFI CTTAAG 1 cut(s) 1319
BstAPI GCANNNNNTGC 1 cut(s) 1246
BstBI TTCGAA 1 cut(s) 521
BstC8I GCNNGC 4 cut(s) 71, 1372, 1391, 1533
BstDSI CCRYGG 1 cut(s) 217
BstF5I GGATG 4 cut(s) 127, 601, 657, 1225
BstFNI CGCG 5 cut(s) 60, 73, 219, 294, 303
BstH2I RGCGCY 1 cut(s) 396
BstHHI GCGC 3 cut(s) 150, 294, 395
BstKTI GATC 4 cut(s) 13, 301, 1189, 1480
BstMAI GTCTC 2 cut(s) 669, 801
BstMBI GATC 4 cut(s) 10, 298, 1186, 1477
BstNSI RCATGY 1 cut(s) 1472
BstSCI CCNGG 2 cut(s) 603, 880
BstSFI CTRYAG 2 cut(s) 343, 1405
BstSLI GKGCMC 1 cut(s) 212
BstUI CGCG 5 cut(s) 60, 73, 219, 294, 303
BstV1I GCAGC 7 cut(s) 205, 329, 542, 670, 1376, 1379, 1522
BstV2I GAAGAC 1 cut(s) 489
BstX2I RGATCY 1 cut(s) 1477
BstXI CCANNNNNNTGG 1 cut(s) 982
BstYI RGATCY 1 cut(s) 1477
Bsu15I ATCGAT 1 cut(s) 1126
BsuI GTATCC 1 cut(s) 601
BsuRI GGCC 2 cut(s) 210, 229
BsuTUI ATCGAT 1 cut(s) 1126
BtgI CCRYGG 1 cut(s) 217
BtgZI GCGATG 2 cut(s) 69, 754
BtsCI GGATG 4 cut(s) 127, 601, 657, 1225
BtsI GCAGTG 2 cut(s) 640, 1142
BtsIMutI CAGTG 5 cut(s) 438, 555, 640, 872, 1142
Cac8I GCNNGC 4 cut(s) 71, 1372, 1391, 1533
CfoI GCGC 3 cut(s) 150, 294, 395
Cfr10I RCCGGY 1 cut(s) 139
Cfr13I GGNCC 3 cut(s) 208, 209, 228
Cfr42I CCGCGG 1 cut(s) 220
ClaI ATCGAT 1 cut(s) 1126
CseI GACGC 1 cut(s) 292
Csp6I GTAC 3 cut(s) 143, 718, 926
CviAII CATG 5 cut(s) 721, 1288, 1360, 1375, 1469
CviQI GTAC 3 cut(s) 143, 718, 926
DpnI GATC 4 cut(s) 12, 300, 1188, 1479
DpnII GATC 4 cut(s) 10, 298, 1186, 1477
Eam1104I CTCTTC 6 cut(s) 40, 238, 478, 649, 742, 1467
EarI CTCTTC 6 cut(s) 40, 238, 478, 649, 742, 1467
EciI GGCGGA 1 cut(s) 1451
Eco24I GRGCYC 3 cut(s) 212, 844, 1082
Eco31I GGTCTC 1 cut(s) 669
Eco32I GATATC 1 cut(s) 783
Eco57I CTGAAG 1 cut(s) 630
EcoO109I RGGNCCY 1 cut(s) 209
EcoRV GATATC 1 cut(s) 783
EcoT38I GRGCYC 3 cut(s) 212, 844, 1082
FaeI CATG 5 cut(s) 724, 1291, 1363, 1378, 1472
FalI AAGNNNNNCTT 4 cut(s) 1266, 1298, 1283, 1315
FaqI GGGAC 2 cut(s) 766, 948
FatI CATG 5 cut(s) 720, 1287, 1359, 1374, 1468
FauI CCCGC 3 cut(s) 65, 224, 416
Fnu4HI GCNGC 7 cut(s) 194, 343, 531, 684, 1365, 1368, 1536
FokI GGATG 4 cut(s) 114, 608, 664, 1232
FriOI GRGCYC 3 cut(s) 212, 844, 1082
Fsp4HI GCNGC 7 cut(s) 194, 343, 531, 684, 1365, 1368, 1536
FspBI CTAG 2 cut(s) 1155, 1628
GlaI GCGC 3 cut(s) 149, 293, 394
GluI GCNGC 7 cut(s) 194, 343, 531, 684, 1365, 1368, 1536
HaeII RGCGCY 1 cut(s) 396
HaeIII GGCC 2 cut(s) 210, 229
HapII CCGG 4 cut(s) 140, 605, 881, 1111
HgaI GACGC 1 cut(s) 292
HhaI GCGC 3 cut(s) 150, 294, 395
Hin1I GRCGYC 1 cut(s) 755
Hin1II CATG 5 cut(s) 724, 1291, 1363, 1378, 1472
Hin6I GCGC 3 cut(s) 148, 292, 393
HinP1I GCGC 3 cut(s) 148, 292, 393
HinfI GANTC 7 cut(s) 203, 317, 617, 623, 761, 1171, 1331
HpaII CCGG 4 cut(s) 140, 605, 881, 1111
HphI GGTGA 5 cut(s) 31, 153, 808, 1474, 1509
Hpy166II GTNNAC 2 cut(s) 553, 1639
Hpy188III TCNNGA 4 cut(s) 459, 786, 1018, 1601
Hpy8I GTNNAC 2 cut(s) 553, 1639
Hpy99I CGWCG 6 cut(s) 121, 203, 247, 496, 760, 904
HpyAV CCTTC 2 cut(s) 330, 1418
HpyCH4III ACNGT 3 cut(s) 414, 867, 1548
HpyCH4IV ACGT 3 cut(s) 310, 403, 755
HpyCH4V TGCA 9 cut(s) 345, 633, 1135, 1148, 1374, 1393, 1407, 1430, 1571
HpySE526I ACGT 3 cut(s) 310, 403, 755
Hsp92I GRCGYC 1 cut(s) 755
Hsp92II CATG 5 cut(s) 724, 1291, 1363, 1378, 1472
HspAI GCGC 3 cut(s) 148, 292, 393
KspI CCGCGG 1 cut(s) 220
Kzo9I GATC 4 cut(s) 10, 298, 1186, 1477
LguI GCTCTTC 3 cut(s) 40, 649, 1467
LmnI GCTCC 4 cut(s) 117, 339, 538, 1543
Lsp1109I GCAGC 7 cut(s) 205, 329, 542, 670, 1376, 1379, 1522
LweI GCATC 3 cut(s) 163, 751, 1210
MaeI CTAG 2 cut(s) 1155, 1628
MaeII ACGT 3 cut(s) 310, 403, 755
MaeIII GTNAC 6 cut(s) 19, 404, 635, 1233, 1266, 1542
MalI GATC 4 cut(s) 12, 300, 1188, 1479
MboI GATC 4 cut(s) 10, 298, 1186, 1477
MflI RGATCY 1 cut(s) 1477
MhlI GDGCHC 3 cut(s) 212, 844, 1082
MluCI AATT 7 cut(s) 463, 589, 812, 1063, 1510, 1553, 1580
MmeI TCCRAC 5 cut(s) 331, 342, 738, 987, 1530
MroXI GAANNNNTTC 3 cut(s) 812, 992, 1618
MseI TTAA 3 cut(s) 822, 1320, 1494
MspA1I CMGCKG 2 cut(s) 219, 683
MspCI CTTAAG 1 cut(s) 1319
MspI CCGG 4 cut(s) 140, 605, 881, 1111
MspR9I CCNGG 2 cut(s) 605, 882
Mva1269I GAATGC 2 cut(s) 1240, 1393
MvnI CGCG 5 cut(s) 60, 73, 219, 294, 303
NciI CCSGG 2 cut(s) 605, 882
NdeII GATC 4 cut(s) 10, 298, 1186, 1477
NlaIII CATG 5 cut(s) 724, 1291, 1363, 1378, 1472
NlaIV GGNNCC 3 cut(s) 210, 211, 1413
NmuCI GTSAC 3 cut(s) 19, 404, 635
NspI RCATGY 1 cut(s) 1472
NspV TTCGAA 1 cut(s) 521
PciSI GCTCTTC 3 cut(s) 40, 649, 1467
PcsI WCGNNNNNNNCGW 2 cut(s) 291, 307
PctI GAATGC 2 cut(s) 1240, 1393
PdmI GAANNNNTTC 3 cut(s) 812, 992, 1618
PfeI GAWTC 7 cut(s) 203, 317, 617, 623, 761, 1171, 1331
PflFI GACNNNGTC 1 cut(s) 904
PfoI TCCNGGA 1 cut(s) 603
PkrI GCNGC 7 cut(s) 195, 344, 532, 685, 1366, 1369, 1537
PspN4I GGNNCC 3 cut(s) 210, 211, 1413
PspOMI GGGCCC 1 cut(s) 208
PspPI GGNCC 3 cut(s) 208, 209, 228
PsrI GAACNNNNNNTAC 2 cut(s) 393, 425
PstI CTGCAG 2 cut(s) 347, 1409
PsuI RGATCY 1 cut(s) 1477
PsyI GACNNNGTC 1 cut(s) 904
PvuII CAGCTG 1 cut(s) 683
RsaI GTAC 3 cut(s) 144, 719, 927
RsaNI GTAC 3 cut(s) 143, 718, 926
SacII CCGCGG 1 cut(s) 220
SapI GCTCTTC 3 cut(s) 40, 649, 1467
SaqAI TTAA 3 cut(s) 822, 1320, 1494
SatI GCNGC 7 cut(s) 194, 343, 531, 684, 1365, 1368, 1536
Sau3AI GATC 4 cut(s) 10, 298, 1186, 1477
Sau96I GGNCC 3 cut(s) 208, 209, 228
ScrFI CCNGG 2 cut(s) 605, 882
SduI GDGCHC 3 cut(s) 212, 844, 1082
SfaNI GCATC 3 cut(s) 163, 751, 1210
SfcI CTRYAG 2 cut(s) 343, 1405
Sfr303I CCGCGG 1 cut(s) 220
SfuI TTCGAA 1 cut(s) 521
SgrBI CCGCGG 1 cut(s) 220
SmlI CTYRAG 3 cut(s) 1081, 1319, 1601
SmoI CTYRAG 3 cut(s) 1081, 1319, 1601
Sse9I AATT 7 cut(s) 463, 589, 812, 1063, 1510, 1553, 1580
SsiI CCGC 8 cut(s) 58, 73, 217, 219, 423, 1141, 1436, 1559
SspMI CTAG 2 cut(s) 1155, 1628
StyD4I CCNGG 2 cut(s) 603, 880
TaaI ACNGT 3 cut(s) 414, 867, 1548
TaiI ACGT 3 cut(s) 313, 406, 758
TaqI TCGA 9 cut(s) 198, 258, 428, 521, 745, 941, 968, 1126, 1465
TasI AATT 7 cut(s) 463, 589, 812, 1063, 1510, 1553, 1580
TatI WGTACW 1 cut(s) 717
TfiI GAWTC 7 cut(s) 203, 317, 617, 623, 761, 1171, 1331
Tru1I TTAA 3 cut(s) 822, 1320, 1494
Tru9I TTAA 3 cut(s) 822, 1320, 1494
TscAI CASTG 5 cut(s) 445, 555, 640, 872, 1142
TseFI GTSAC 3 cut(s) 19, 404, 635
TseI GCWGC 7 cut(s) 193, 342, 530, 683, 1364, 1367, 1535
Tsp45I GTSAC 3 cut(s) 19, 404, 635
TspDTI ATGAA 7 cut(s) 17, 737, 1109, 1244, 1291, 1304, 1394
TspGWI ACGGA 2 cut(s) 1234, 1530
TspRI CASTG 5 cut(s) 445, 555, 640, 872, 1142
Tth111I GACNNNGTC 1 cut(s) 904
Vha464I CTTAAG 1 cut(s) 1319
XapI RAATTY 3 cut(s) 463, 589, 812
XceI RCATGY 1 cut(s) 1472
XcmI CCANNNNNNNNNTGG 1 cut(s) 1332
XmnI GAANNNNTTC 3 cut(s) 812, 992, 1618
XspI CTAG 2 cut(s) 1155, 1628
ZraI GACGTC 1 cut(s) 756
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.