Rw2G046320

QWRF motif-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0010969
Physical Location & Seq
Forward (+)
73615182 .. 73616653
1472 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G046320.1

Sequence Viewer

Length: 804 bp
ATGAACAAGTTTAACCTGAAAAATGTGATCAAGAGGGCTAATTCGTTGACCGGGTCAAAGTTCGGGACGACGCAGTGGGCATTGTCGCCGGGAAGGAGCGTGGCGCAGCCGGGGTCGGCGGAGAGTTTGGGGAAGTCGACGTCGTTTTCGAGCTTGAAGCCTCCGACGAGTCCTTCAAAAGTAAGGGAAACATTGATGCAATGGCGGTACGCAAATGCAAGAGCTGAAACTGTGAATGGCAATGTTGCTGATCAAGCACAGGTCAATTTTTTGAGTGCTTGTAATAGTCTTATGAAGTTGCGGCAATCCGTGCTGCATAAGAAACTGAAGCTTCAGAAAGAAAGGCTCGAAATGAAGCTGAACTTTACACTACATTCTCAATTTGAGCCATTGGAAGCTTGGGGAGATATGGAAAGGCAACATATGGGAGCAGTTTCTATGTTGAAAGAGAGCTTACATTCTGTTGTGTGCAGAGTGCCTCTCACTGAAGGTGCAAAGGTGGATACTCAATCAGCCGCCATCAATCTTCGAAATGCTTCAGATTACTCAGCCTCCATTAAGTTGATATTGGCTACATTTGCCCCCTCGGCTGAGCAGACTAGTGTGTTGCTCTCAGAATTAGCGGAGGTTGTTGCGCAAGAAAAGTTGCTTTCTGATGAGTGTCTTGAGCTCTGTAGAACCATATCAGAACTAGAGATGGAAGAGAGGGGATTGAAGTGTAGTATTATTCAATTGGAATCATGGAAACAAGAGCAAATTCAGCTGCAGCAGCAGGAATCAAGACAACAAGAGATCATGCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
QWRF

Protein Analysis

267

Amino Acids

29.83

Weight (kDa)

8.55

Isoelectric Point (pI)

43.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
QWRF PF04484 65 - 217 2e-51 QWRF family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 143
Acc16I TGCGCA 1 cut(s) 636
AccI GTMKAC 1 cut(s) 137
AciI CCGC 5 cut(s) 119, 205, 301, 516, 623
AcsI RAATTY 1 cut(s) 756
AcuI CTGAAG 4 cut(s) 317, 347, 507, 522
AcyI GRCGYC 1 cut(s) 140
AfaI GTAC 1 cut(s) 209
AgsI TTSAA 5 cut(s) 157, 177, 445, 715, 731
AhlI ACTAGT 1 cut(s) 599
AluBI AGCT 8 cut(s) 153, 224, 331, 358, 398, 453, 670, 763
AluI AGCT 8 cut(s) 153, 224, 331, 358, 398, 453, 670, 763
Alw21I GWGCWC 1 cut(s) 672
ApeKI GCWGC 5 cut(s) 106, 313, 763, 766, 769
ApoI RAATTY 1 cut(s) 756
ArsI GACNNNNNNTTYG 2 cut(s) 130, 162
Asp700I GAANNNNTTC 1 cut(s) 535
AspLEI GCGC 2 cut(s) 106, 637
AsuC2I CCSGG 3 cut(s) 52, 90, 111
AsuII TTCGAA 1 cut(s) 529
BanII GRGCYC 1 cut(s) 672
Bbv12I GWGCWC 1 cut(s) 672
BbvI GCAGC 5 cut(s) 118, 300, 750, 778, 781
BccI CCATC 2 cut(s) 527, 691
BciVI GTATCC 1 cut(s) 496
BclI TGATCA 2 cut(s) 27, 250
BcnI CCSGG 3 cut(s) 52, 90, 111
BcuI ACTAGT 1 cut(s) 599
BfaI CTAG 2 cut(s) 600, 692
BfmI CTRYAG 2 cut(s) 673, 764
BfuI GTATCC 1 cut(s) 496
BglI GCCNNNNNGGC 1 cut(s) 587
BisI GCNGC 7 cut(s) 107, 302, 314, 516, 764, 767, 770
BlpI GCTNAGC 1 cut(s) 591
BlsI GCNGC 7 cut(s) 108, 303, 315, 517, 765, 768, 771
Bme1390I CCNGG 3 cut(s) 52, 90, 111
BmrFI CCNGG 3 cut(s) 52, 90, 111
BmsI GCATC 1 cut(s) 186
BplI GAGNNNNNCTC 2 cut(s) 465, 497
Bpu1102I GCTNAGC 1 cut(s) 591
Bpu14I TTCGAA 1 cut(s) 529
BpuEI CTTGAG 1 cut(s) 686
BpuMI CCSGG 3 cut(s) 52, 90, 111
BsaHI GRCGYC 1 cut(s) 140
BsaJI CCNNGG 2 cut(s) 110, 585
BsaXI ACNNNNNCTCC 2 cut(s) 536, 566
Bse3DI GCAATG 2 cut(s) 206, 247
BseDI CCNNGG 2 cut(s) 110, 585
BseMI GCAATG 2 cut(s) 206, 247
BseMII CTCAG 3 cut(s) 561, 582, 627
BseXI GCAGC 5 cut(s) 118, 300, 750, 778, 781
BsgI GTGCAG 1 cut(s) 490
BsiHKAI GWGCWC 1 cut(s) 672
BsiSI CCGG 3 cut(s) 51, 89, 110
BslFI GGGAC 1 cut(s) 79
BsmFI GGGAC 1 cut(s) 79
Bsp119I TTCGAA 1 cut(s) 529
Bsp1286I GDGCHC 1 cut(s) 672
Bsp143I GATC 3 cut(s) 27, 250, 792
Bsp1720I GCTNAGC 1 cut(s) 591
BspACI CCGC 5 cut(s) 119, 205, 301, 516, 623
BspCNI CTCAG 3 cut(s) 560, 583, 626
BspMAI CTGCAG 1 cut(s) 768
BspT104I TTCGAA 1 cut(s) 529
BsrDI GCAATG 2 cut(s) 206, 247
BssECI CCNNGG 2 cut(s) 110, 585
BssMI GATC 3 cut(s) 27, 250, 792
BssNI GRCGYC 1 cut(s) 140
Bst4CI ACNGT 1 cut(s) 232
Bst6I CTCTTC 1 cut(s) 696
BstACI GRCGYC 1 cut(s) 140
BstAPI GCANNNNNTGC 1 cut(s) 310
BstBI TTCGAA 1 cut(s) 529
BstDEI CTNAG 3 cut(s) 547, 591, 613
BstHHI GCGC 2 cut(s) 106, 637
BstKTI GATC 3 cut(s) 30, 253, 795
BstMBI GATC 3 cut(s) 27, 250, 792
BstMWI GCNNNNNNNGC 6 cut(s) 254, 310, 578, 587, 760, 769
BstSCI CCNGG 3 cut(s) 50, 88, 109
BstSFI CTRYAG 2 cut(s) 673, 764
BstV1I GCAGC 5 cut(s) 118, 300, 750, 778, 781
BsuI GTATCC 1 cut(s) 496
BtsI GCAGTG 1 cut(s) 80
BtsIMutI CAGTG 2 cut(s) 80, 483
CfoI GCGC 2 cut(s) 106, 637
CseI GACGC 1 cut(s) 79
Csp6I GTAC 1 cut(s) 208
CviAII CATG 3 cut(s) 741, 796, 801
CviQI GTAC 1 cut(s) 208
DdeI CTNAG 3 cut(s) 547, 591, 613
DpnI GATC 3 cut(s) 29, 252, 794
DpnII GATC 3 cut(s) 27, 250, 792
Eam1104I CTCTTC 1 cut(s) 696
EarI CTCTTC 1 cut(s) 696
EciI GGCGGA 1 cut(s) 134
Ecl136II GAGCTC 1 cut(s) 670
Eco24I GRGCYC 1 cut(s) 672
Eco53kI GAGCTC 1 cut(s) 670
Eco57I CTGAAG 4 cut(s) 317, 347, 507, 522
EcoICRI GAGCTC 1 cut(s) 670
EcoT38I GRGCYC 1 cut(s) 672
FaeI CATG 3 cut(s) 744, 799, 804
FalI AAGNNNNNCTT 2 cut(s) 347, 379
FaqI GGGAC 1 cut(s) 79
FatI CATG 3 cut(s) 740, 795, 800
FauNDI CATATG 1 cut(s) 423
FbaI TGATCA 2 cut(s) 27, 250
FblI GTMKAC 1 cut(s) 137
Fnu4HI GCNGC 7 cut(s) 107, 302, 314, 516, 764, 767, 770
FriOI GRGCYC 1 cut(s) 672
Fsp4HI GCNGC 7 cut(s) 107, 302, 314, 516, 764, 767, 770
FspBI CTAG 2 cut(s) 600, 692
FspI TGCGCA 1 cut(s) 636
GlaI GCGC 2 cut(s) 105, 636
GluI GCNGC 7 cut(s) 107, 302, 314, 516, 764, 767, 770
HapII CCGG 3 cut(s) 51, 89, 110
HgaI GACGC 1 cut(s) 79
HhaI GCGC 2 cut(s) 106, 637
Hin1I GRCGYC 1 cut(s) 140
Hin1II CATG 3 cut(s) 744, 799, 804
Hin6I GCGC 2 cut(s) 104, 635
HinP1I GCGC 2 cut(s) 104, 635
HincII GTYRAC 2 cut(s) 48, 138
HindII GTYRAC 2 cut(s) 48, 138
HindIII AAGCTT 2 cut(s) 329, 396
HinfI GANTC 3 cut(s) 169, 737, 776
HpaII CCGG 3 cut(s) 51, 89, 110
Hpy166II GTNNAC 2 cut(s) 48, 138
Hpy188I TCNGA 6 cut(s) 165, 336, 541, 616, 655, 688
Hpy188III TCNNGA 4 cut(s) 31, 64, 665, 780
Hpy8I GTNNAC 2 cut(s) 48, 138
Hpy99I CGWCG 4 cut(s) 73, 142, 145, 169
HpyAV CCTTC 3 cut(s) 87, 183, 482
HpyCH4III ACNGT 1 cut(s) 232
HpyCH4IV ACGT 1 cut(s) 140
HpyCH4V TGCA 6 cut(s) 199, 218, 316, 471, 494, 766
HpyF10VI GCNNNNNNNGC 6 cut(s) 254, 310, 578, 587, 760, 769
HpyF3I CTNAG 3 cut(s) 547, 591, 613
HpySE526I ACGT 1 cut(s) 140
Hsp92I GRCGYC 1 cut(s) 140
Hsp92II CATG 3 cut(s) 744, 799, 804
HspAI GCGC 2 cut(s) 104, 635
Ksp22I TGATCA 2 cut(s) 27, 250
Kzo9I GATC 3 cut(s) 27, 250, 792
LmnI GCTCC 2 cut(s) 96, 428
LpnPI CCDG 6 cut(s) 29, 64, 102, 123, 245, 758
Lsp1109I GCAGC 5 cut(s) 118, 300, 750, 778, 781
LweI GCATC 1 cut(s) 186
MaeI CTAG 2 cut(s) 600, 692
MaeII ACGT 1 cut(s) 140
MalI GATC 3 cut(s) 29, 252, 794
MboI GATC 3 cut(s) 27, 250, 792
MboII GAAGA 2 cut(s) 518, 713
MfeI CAATTG 1 cut(s) 731
MhlI GDGCHC 1 cut(s) 672
MluCI AATT 6 cut(s) 40, 265, 380, 617, 731, 756
MlyI GAGTC 1 cut(s) 178
MmeI TCCRAC 1 cut(s) 188
MnlI CCTC 7 cut(s) 27, 171, 489, 562, 595, 619, 699
MroXI GAANNNNTTC 1 cut(s) 535
MseI TTAA 2 cut(s) 12, 558
MspA1I CMGCKG 1 cut(s) 763
MspI CCGG 3 cut(s) 51, 89, 110
MspR9I CCNGG 3 cut(s) 52, 90, 111
MunI CAATTG 1 cut(s) 731
MwoI GCNNNNNNNGC 6 cut(s) 254, 310, 578, 587, 760, 769
NciI CCSGG 3 cut(s) 52, 90, 111
NdeI CATATG 1 cut(s) 423
NdeII GATC 3 cut(s) 27, 250, 792
NlaIII CATG 3 cut(s) 744, 799, 804
NmeAIII GCCGAG 1 cut(s) 566
NsbI TGCGCA 1 cut(s) 636
NspV TTCGAA 1 cut(s) 529
PcsI WCGNNNNNNNCGW 1 cut(s) 146
PdmI GAANNNNTTC 1 cut(s) 535
PfeI GAWTC 2 cut(s) 737, 776
PflFI GACNNNGTC 1 cut(s) 52
PkrI GCNGC 7 cut(s) 108, 303, 315, 517, 765, 768, 771
PleI GAGTC 1 cut(s) 177
PpsI GAGTC 1 cut(s) 177
Psp124BI GAGCTC 1 cut(s) 672
PstI CTGCAG 1 cut(s) 768
PsyI GACNNNGTC 1 cut(s) 52
PvuII CAGCTG 1 cut(s) 763
RsaI GTAC 1 cut(s) 209
RsaNI GTAC 1 cut(s) 208
SacI GAGCTC 1 cut(s) 672
SalI GTCGAC 1 cut(s) 136
SaqAI TTAA 2 cut(s) 12, 558
SatI GCNGC 7 cut(s) 107, 302, 314, 516, 764, 767, 770
Sau3AI GATC 3 cut(s) 27, 250, 792
SchI GAGTC 1 cut(s) 178
ScrFI CCNGG 3 cut(s) 52, 90, 111
SduI GDGCHC 1 cut(s) 672
SfaNI GCATC 1 cut(s) 186
SfcI CTRYAG 2 cut(s) 673, 764
SfuI TTCGAA 1 cut(s) 529
SmlI CTYRAG 1 cut(s) 665
SmoI CTYRAG 1 cut(s) 665
SpeI ACTAGT 1 cut(s) 599
Sse9I AATT 6 cut(s) 40, 265, 380, 617, 731, 756
SsiI CCGC 5 cut(s) 119, 205, 301, 516, 623
SspMI CTAG 2 cut(s) 600, 692
SstI GAGCTC 1 cut(s) 672
StyD4I CCNGG 3 cut(s) 50, 88, 109
TaaI ACNGT 1 cut(s) 232
TaiI ACGT 1 cut(s) 143
TaqI TCGA 4 cut(s) 137, 149, 348, 529
TasI AATT 6 cut(s) 40, 265, 380, 617, 731, 756
TauI GCSGC 2 cut(s) 304, 518
TfiI GAWTC 2 cut(s) 737, 776
Tru1I TTAA 2 cut(s) 12, 558
Tru9I TTAA 2 cut(s) 12, 558
TscAI CASTG 2 cut(s) 80, 490
TseI GCWGC 5 cut(s) 106, 313, 763, 766, 769
TspDTI ATGAA 3 cut(s) 17, 308, 368
TspGWI ACGGA 1 cut(s) 298
TspRI CASTG 2 cut(s) 80, 490
Tth111I GACNNNGTC 1 cut(s) 52
XapI RAATTY 1 cut(s) 756
XcmI CCANNNNNNNNNTGG 1 cut(s) 396
XmiI GTMKAC 1 cut(s) 137
XmnI GAANNNNTTC 1 cut(s) 535
XspI CTAG 2 cut(s) 600, 692
ZraI GACGTC 1 cut(s) 141
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.