MD09G1092900.v1.1

QWRF motif-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0010969
Physical Location & Seq
Reverse (-)
6803235 .. 6807730
4496 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1092900.v1.1.491

Sequence Viewer

Length: 1668 bp
ATGTGGTGGTTTGTTTTTGTCTCATCTGTTTCCCAAACGTACGCAGAGAAAATCAAATTCTCAAATCCTCCATTTCTCACACACACAAGTCTGGTAACAGACCAATTCCACAAACCCAGAAGACCCAAATACCGGGAAGTAAGCTCTCGGTTTCTATCGTCGCCAACCACCGCAGAAACCACCACGCTCCCATCACCGAACCACCCCATCTCGCCGGTCCGCCGCAAACCCAAAATCGACGATTCGGGCCCCAACCGCGGACTATGGCCCTCCTCAATGTCGTCGCACAAGAAACTCGACACTCTCGCCGACCACCTCGGAAACGACGGGCGAGAAGATCGAATCGAACGTAAAGGTTCAATCTTTGACAGACAGAGGAGCTACAGAGAGTTTGGTTTGTTGGATAATCCGAAAGAAAACGCCAAAGAAAATCACAGACCCATTTTTGGTGGGTCAATGAGATACACTGGAAAATTCAGTTTTCCCGGAAAATCTAACTCGTCGTCTTCTTCTACTTCGTCGTCTTCGAGCAAGTTTTCGAGCAATTCAAATGTGGCTGCTCCTCGGAGATTTTCAGTGGACGAAAATGCTCTGCATCAGAAGTCGTCTCGCCGAATTTCGGATGCTCTTGATTCGGGATCGGAGTGCAGTGACGGCTATTCAGGGATGACCATGGGGTCTCCAAATGTCGGTCAGAGTGCTCGAAAATCGGGTGTGGAGGTTTCTTCTAAGTACATGAATGAGATTACCTTAAGGCATCGGAGACGGACATCGGATTCTAACATCGCAAATCCGATATCGGGAGACAAGTCTCCGAAGCTGAACAAATTCACCATTAAAAATGTGATCAGGAGGGCTCATTCGCTGACAGCGACCACACAGTGGGCGCTTTCACCGGGAAGAACAGGGTCGCCGACGATGTCTGTGGAGAATAAGGGAGTTCAATTGTCGTCTTCGAGCATGAAGCCTCCTATGAGTCCCTCAAAAACCAAAGGGGTGGAGAAGTTGCTCAACTTGGGGTTGGACTTGTTCAAGACTAAAAAATCTTCTTCTTCGCCGTCTAGTGTGTTGGTGAGGTATGGTTCAGTTTCTTCGGGTTCAAGTATGACAGAGATGGGTCATCAGCTTCGGTTGCTTCATAATCGAATCTTGCAGTGGCGGTTTGCGAATGCTAGAGCTGATGTTGTGAATCAGAACATAGCAAATCAAGCACAGATCAATGTTTTATATGCTTGGGACGGTTTGATGAAGTTGCAACATTCCGTGCTACAAAAGAAAGTGAAGCTACATAAAGAAAAGCTTGACATGAAGCTGAACTTTATACTTTATTCCCAACTTAAGCCGTTGGCGTCTTGGGGAGATATGGAAAGGCAACATACTGCAGCAGTGTGCAGGATGAAAGAGTGCTTGCATTCTGTTGTTTGCAGGGTGCCCCTCGTTGAAGATGCAGAGGTGGACACTCAATCTGCTTCCACTGCTCTTCGACATGCTTCGGATCTCACAGCTTCCATTAAGTTGTTGCTTGCTGGTTTTTCGCCATTGGCTGAGCAAGCTGCTCCTTTACTATCGCAACTAGCGGAGGTGGTTGCACAAGAAAAATTGCTTTTAGAGGAGTGTATTGAGCTTTTCCGAAACATATCCACGCTAGAGGTAATAAACGGACGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
QWRF

Protein Analysis

556

Amino Acids

61.63

Weight (kDa)

9.92

Isoelectric Point (pI)

55.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
QWRF PF04484 229 - 537 9.3e-80 QWRF family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 676
AccB1I GGYRCC 1 cut(s) 1429
AccB7I CCANNNNNTGG 1 cut(s) 882
AccII CGCG 1 cut(s) 258
AciI CCGC 7 cut(s) 171, 220, 223, 256, 258, 1159, 1577
AclWI GGATC 2 cut(s) 646, 1503
AcsI RAATTY 4 cut(s) 56, 473, 615, 827
AcyI GRCGYC 1 cut(s) 1349
AdeI CACNNNGTG 1 cut(s) 882
AfaI GTAC 2 cut(s) 41, 734
AfiI CCNNNNNNNGG 7 cut(s) 132, 257, 446, 619, 689, 800, 882
AflII CTTAAG 2 cut(s) 751, 1337
AgsI TTSAA 6 cut(s) 360, 549, 944, 1033, 1101, 1442
Alw21I GWGCWC 1 cut(s) 703
Alw26I GTCTC 6 cut(s) 25, 612, 684, 757, 798, 816
AlwI GGATC 2 cut(s) 646, 1503
AoxI GGCC 2 cut(s) 247, 266
ApaI GGGCCC 1 cut(s) 251
ApeKI GCWGC 3 cut(s) 557, 1382, 1553
ApoI RAATTY 4 cut(s) 56, 473, 615, 827
Asp700I GAANNNNTTC 1 cut(s) 827
AspLEI GCGC 1 cut(s) 889
AspS9I GGNCC 4 cut(s) 217, 247, 248, 267
AsuC2I CCSGG 3 cut(s) 134, 486, 897
AsuHPI GGTGA 4 cut(s) 186, 823, 885, 1084
AvaII GGWCC 1 cut(s) 217
BaeGI GKGCMC 2 cut(s) 251, 1434
BanI GGYRCC 1 cut(s) 1429
BanII GRGCYC 2 cut(s) 251, 859
BbsI GAAGAC 4 cut(s) 127, 498, 516, 945
Bbv12I GWGCWC 1 cut(s) 703
BbvI GCAGC 3 cut(s) 544, 1394, 1540
BccI CCATC 3 cut(s) 199, 215, 1108
BceAI ACGGC 3 cut(s) 670, 1042, 1327
BclI TGATCA 1 cut(s) 846
BcnI CCSGG 3 cut(s) 134, 486, 897
BcoDI GTCTC 6 cut(s) 25, 612, 684, 757, 798, 816
BfaI CTAG 4 cut(s) 1062, 1173, 1574, 1646
BfmI CTRYAG 2 cut(s) 382, 1380
BfoI RGCGCY 1 cut(s) 890
BfrI CTTAAG 2 cut(s) 751, 1337
BisI GCNGC 4 cut(s) 223, 558, 1383, 1554
BlpI GCTNAGC 1 cut(s) 1545
BlsI GCNGC 4 cut(s) 224, 559, 1384, 1555
Bme1390I CCNGG 3 cut(s) 134, 486, 897
Bme18I GGWCC 1 cut(s) 217
BmgT120I GGNCC 4 cut(s) 217, 247, 248, 267
BmiI GGNNCC 3 cut(s) 249, 250, 1431
BmrFI CCNGG 3 cut(s) 134, 486, 897
BmsI GCATC 4 cut(s) 604, 613, 766, 1435
BpiI GAAGAC 4 cut(s) 127, 498, 516, 945
Bpu1102I GCTNAGC 1 cut(s) 1545
BpuMI CCSGG 3 cut(s) 134, 486, 897
BsaHI GRCGYC 1 cut(s) 1349
BsaI GGTCTC 1 cut(s) 684
BsaJI CCNNGG 4 cut(s) 256, 316, 563, 672
Bsc4I CCNNNNNNNGG 7 cut(s) 132, 257, 446, 619, 689, 800, 882
Bse118I RCCGGY 1 cut(s) 214
Bse1I ACTGG 1 cut(s) 472
BseDI CCNNGG 4 cut(s) 256, 316, 563, 672
BseGI GGATG 3 cut(s) 628, 672, 1401
BseLI CCNNNNNNNGG 7 cut(s) 132, 257, 446, 619, 689, 800, 882
BseMII CTCAG 1 cut(s) 1536
BseNI ACTGG 1 cut(s) 472
BseRI GAGGAG 4 cut(s) 262, 391, 552, 1625
BseSI GKGCMC 2 cut(s) 251, 1434
BseXI GCAGC 3 cut(s) 544, 1394, 1540
BsgI GTGCAG 2 cut(s) 667, 1411
Bsh1236I CGCG 1 cut(s) 258
BshFI GGCC 2 cut(s) 249, 268
BshNI GGYRCC 1 cut(s) 1429
BsiHKAI GWGCWC 1 cut(s) 703
BsiSI CCGG 4 cut(s) 133, 215, 486, 896
BsiWI CGTACG 1 cut(s) 39
BslFI GGGAC 2 cut(s) 963, 1250
BslI CCNNNNNNNGG 7 cut(s) 132, 257, 446, 619, 689, 800, 882
BsmAI GTCTC 6 cut(s) 25, 612, 684, 757, 798, 816
BsmBI CGTCTC 2 cut(s) 612, 757
BsmFI GGGAC 2 cut(s) 963, 1250
BsmI GAATGC 2 cut(s) 1174, 1411
BsnI GGCC 2 cut(s) 249, 268
Bso31I GGTCTC 1 cut(s) 684
Bsp120I GGGCCC 1 cut(s) 247
Bsp1286I GDGCHC 4 cut(s) 251, 703, 859, 1434
Bsp143I GATC 5 cut(s) 337, 638, 846, 1215, 1495
Bsp1720I GCTNAGC 1 cut(s) 1545
Bsp19I CCATGG 1 cut(s) 672
BspACI CCGC 7 cut(s) 171, 220, 223, 256, 258, 1159, 1577
BspANI GGCC 2 cut(s) 249, 268
BspCNI CTCAG 1 cut(s) 1537
BspFNI CGCG 1 cut(s) 258
BspLI GGNNCC 3 cut(s) 249, 250, 1431
BspMAI CTGCAG 1 cut(s) 1384
BspPI GGATC 2 cut(s) 646, 1503
BspQI GCTCTTC 1 cut(s) 1485
BspT107I GGYRCC 1 cut(s) 1429
BspTI CTTAAG 2 cut(s) 751, 1337
BspTNI GGTCTC 1 cut(s) 684
BsrFI RCCGGY 1 cut(s) 214
BsrI ACTGG 1 cut(s) 472
BssAI RCCGGY 1 cut(s) 214
BssECI CCNNGG 4 cut(s) 256, 316, 563, 672
BssMI GATC 5 cut(s) 337, 638, 846, 1215, 1495
BssNI GRCGYC 1 cut(s) 1349
BssT1I CCWWGG 1 cut(s) 672
Bst4CI ACNGT 2 cut(s) 882, 1241
Bst6I CTCTTC 1 cut(s) 1485
BstACI GRCGYC 1 cut(s) 1349
BstAFI CTTAAG 2 cut(s) 751, 1337
BstC8I GCNNGC 3 cut(s) 1409, 1524, 1551
BstDEI CTNAG 2 cut(s) 729, 1545
BstDSI CCRYGG 2 cut(s) 256, 672
BstF5I GGATG 3 cut(s) 628, 672, 1401
BstFNI CGCG 1 cut(s) 258
BstH2I RGCGCY 1 cut(s) 890
BstHHI GCGC 1 cut(s) 889
BstKTI GATC 5 cut(s) 340, 641, 849, 1218, 1498
BstMAI GTCTC 6 cut(s) 25, 612, 684, 757, 798, 816
BstMBI GATC 5 cut(s) 337, 638, 846, 1215, 1495
BstMWI GCNNNNNNNGC 6 cut(s) 255, 654, 1132, 1208, 1475, 1550
BstNSI RCATGY 1 cut(s) 1490
BstSCI CCNGG 3 cut(s) 132, 484, 895
BstSFI CTRYAG 2 cut(s) 382, 1380
BstSLI GKGCMC 2 cut(s) 251, 1434
BstUI CGCG 1 cut(s) 258
BstV1I GCAGC 3 cut(s) 544, 1394, 1540
BstV2I GAAGAC 4 cut(s) 127, 498, 516, 945
BstX2I RGATCY 1 cut(s) 1495
BstXI CCANNNNNNTGG 1 cut(s) 997
BstYI RGATCY 1 cut(s) 1495
BsuRI GGCC 2 cut(s) 249, 268
BtgI CCRYGG 2 cut(s) 256, 672
BtgZI GCGATG 1 cut(s) 769
BtsCI GGATG 3 cut(s) 628, 672, 1401
BtsI GCAGTG 4 cut(s) 655, 1160, 1392, 1473
BtsIMutI CAGTG 7 cut(s) 465, 582, 655, 887, 1160, 1392, 1473
Cac8I GCNNGC 3 cut(s) 1409, 1524, 1551
CfoI GCGC 1 cut(s) 889
Cfr10I RCCGGY 1 cut(s) 214
Cfr13I GGNCC 4 cut(s) 217, 247, 248, 267
Cfr42I CCGCGG 1 cut(s) 259
CpoI CGGWCCG 1 cut(s) 217
CseI GACGC 1 cut(s) 1338
Csp6I GTAC 2 cut(s) 40, 733
CspI CGGWCCG 1 cut(s) 217
CviAII CATG 5 cut(s) 673, 736, 961, 1306, 1487
CviQI GTAC 2 cut(s) 40, 733
DdeI CTNAG 2 cut(s) 729, 1545
DpnI GATC 5 cut(s) 339, 640, 848, 1217, 1497
DpnII GATC 5 cut(s) 337, 638, 846, 1215, 1495
DraIII CACNNNGTG 1 cut(s) 882
DrdI GACNNNNNNGTC 1 cut(s) 676
DseDI GACNNNNNNGTC 1 cut(s) 676
Eam1104I CTCTTC 1 cut(s) 1485
EarI CTCTTC 1 cut(s) 1485
EciI GGCGGA 1 cut(s) 209
Eco130I CCWWGG 1 cut(s) 672
Eco24I GRGCYC 2 cut(s) 251, 859
Eco31I GGTCTC 1 cut(s) 684
Eco32I GATATC 1 cut(s) 798
Eco47I GGWCC 1 cut(s) 217
EcoO109I RGGNCCY 1 cut(s) 248
EcoRV GATATC 1 cut(s) 798
EcoT14I CCWWGG 1 cut(s) 672
EcoT38I GRGCYC 2 cut(s) 251, 859
ErhI CCWWGG 1 cut(s) 672
Esp3I CGTCTC 2 cut(s) 612, 757
FaeI CATG 5 cut(s) 676, 739, 964, 1309, 1490
FalI AAGNNNNNCTT 4 cut(s) 1284, 1316, 1301, 1333
FaqI GGGAC 2 cut(s) 963, 1250
FatI CATG 5 cut(s) 672, 735, 960, 1305, 1486
FbaI TGATCA 1 cut(s) 846
Fnu4HI GCNGC 4 cut(s) 223, 558, 1383, 1554
FokI GGATG 3 cut(s) 635, 679, 1408
FriOI GRGCYC 2 cut(s) 251, 859
Fsp4HI GCNGC 4 cut(s) 223, 558, 1383, 1554
FspBI CTAG 4 cut(s) 1062, 1173, 1574, 1646
GlaI GCGC 1 cut(s) 888
GluI GCNGC 4 cut(s) 223, 558, 1383, 1554
HaeII RGCGCY 1 cut(s) 890
HaeIII GGCC 2 cut(s) 249, 268
HapII CCGG 4 cut(s) 133, 215, 486, 896
HgaI GACGC 1 cut(s) 1338
HhaI GCGC 1 cut(s) 889
Hin1I GRCGYC 1 cut(s) 1349
Hin1II CATG 5 cut(s) 676, 739, 964, 1309, 1490
Hin6I GCGC 1 cut(s) 887
HinP1I GCGC 1 cut(s) 887
HindIII AAGCTT 1 cut(s) 1298
HinfI GANTC 7 cut(s) 242, 342, 632, 776, 976, 1146, 1189
HpaII CCGG 4 cut(s) 133, 215, 486, 896
HphI GGTGA 4 cut(s) 186, 823, 885, 1084
Hpy166II GTNNAC 2 cut(s) 580, 1456
Hpy188III TCNNGA 5 cut(s) 629, 636, 801, 850, 1033
Hpy8I GTNNAC 2 cut(s) 580, 1456
Hpy99I CGWCG 7 cut(s) 163, 242, 286, 329, 505, 523, 919
HpyCH4III ACNGT 2 cut(s) 882, 1241
HpyCH4IV ACGT 2 cut(s) 38, 349
HpyF10VI GCNNNNNNNGC 6 cut(s) 255, 654, 1132, 1208, 1475, 1550
HpyF3I CTNAG 2 cut(s) 729, 1545
HpySE526I ACGT 2 cut(s) 38, 349
Hsp92I GRCGYC 1 cut(s) 1349
Hsp92II CATG 5 cut(s) 676, 739, 964, 1309, 1490
HspAI GCGC 1 cut(s) 887
Ksp22I TGATCA 1 cut(s) 846
KspI CCGCGG 1 cut(s) 259
Kzo9I GATC 5 cut(s) 337, 638, 846, 1215, 1495
LguI GCTCTTC 1 cut(s) 1485
LmnI GCTCC 4 cut(s) 192, 378, 565, 1561
Lsp1109I GCAGC 3 cut(s) 544, 1394, 1540
LweI GCATC 4 cut(s) 604, 613, 766, 1435
MaeI CTAG 4 cut(s) 1062, 1173, 1574, 1646
MaeII ACGT 2 cut(s) 38, 349
MaeIII GTNAC 2 cut(s) 94, 650
MalI GATC 5 cut(s) 339, 640, 848, 1217, 1497
MboI GATC 5 cut(s) 337, 638, 846, 1215, 1495
MfeI CAATTG 1 cut(s) 944
MflI RGATCY 1 cut(s) 1495
MhlI GDGCHC 4 cut(s) 251, 703, 859, 1434
MluCI AATT 8 cut(s) 56, 104, 473, 544, 615, 827, 944, 1598
MlyI GAGTC 1 cut(s) 985
MmeI TCCRAC 2 cut(s) 381, 1002
MroXI GAANNNNTTC 1 cut(s) 827
MseI TTAA 4 cut(s) 752, 837, 1338, 1512
MspA1I CMGCKG 1 cut(s) 258
MspCI CTTAAG 2 cut(s) 751, 1337
MspI CCGG 4 cut(s) 133, 215, 486, 896
MspR9I CCNGG 3 cut(s) 134, 486, 897
MunI CAATTG 1 cut(s) 944
Mva1269I GAATGC 2 cut(s) 1174, 1411
MvnI CGCG 1 cut(s) 258
MwoI GCNNNNNNNGC 6 cut(s) 255, 654, 1132, 1208, 1475, 1550
NciI CCSGG 3 cut(s) 134, 486, 897
NcoI CCATGG 1 cut(s) 672
NdeII GATC 5 cut(s) 337, 638, 846, 1215, 1495
NlaIII CATG 5 cut(s) 676, 739, 964, 1309, 1490
NlaIV GGNNCC 3 cut(s) 249, 250, 1431
NmuCI GTSAC 1 cut(s) 650
NspI RCATGY 1 cut(s) 1490
PciSI GCTCTTC 1 cut(s) 1485
PcsI WCGNNNNNNNCGW 3 cut(s) 346, 524, 869
PctI GAATGC 2 cut(s) 1174, 1411
PdmI GAANNNNTTC 1 cut(s) 827
PfeI GAWTC 6 cut(s) 242, 342, 632, 776, 1146, 1189
Pfl23II CGTACG 1 cut(s) 39
PflFI GACNNNGTC 1 cut(s) 919
PflMI CCANNNNNTGG 1 cut(s) 882
PfoI TCCNGGA 1 cut(s) 484
PkrI GCNGC 4 cut(s) 224, 559, 1384, 1555
PleI GAGTC 1 cut(s) 984
PpsI GAGTC 1 cut(s) 984
PspLI CGTACG 1 cut(s) 39
PspN4I GGNNCC 3 cut(s) 249, 250, 1431
PspOMI GGGCCC 1 cut(s) 247
PspPI GGNCC 4 cut(s) 217, 247, 248, 267
PstI CTGCAG 1 cut(s) 1384
PsuI RGATCY 1 cut(s) 1495
PsyI GACNNNGTC 1 cut(s) 919
RsaI GTAC 2 cut(s) 41, 734
RsaNI GTAC 2 cut(s) 40, 733
Rsr2I CGGWCCG 1 cut(s) 217
RsrII CGGWCCG 1 cut(s) 217
SacII CCGCGG 1 cut(s) 259
SapI GCTCTTC 1 cut(s) 1485
SaqAI TTAA 4 cut(s) 752, 837, 1338, 1512
SatI GCNGC 4 cut(s) 223, 558, 1383, 1554
Sau3AI GATC 5 cut(s) 337, 638, 846, 1215, 1495
Sau96I GGNCC 4 cut(s) 217, 247, 248, 267
SchI GAGTC 1 cut(s) 985
ScrFI CCNGG 3 cut(s) 134, 486, 897
SduI GDGCHC 4 cut(s) 251, 703, 859, 1434
SfaNI GCATC 4 cut(s) 604, 613, 766, 1435
SfcI CTRYAG 2 cut(s) 382, 1380
Sfr303I CCGCGG 1 cut(s) 259
SgrBI CCGCGG 1 cut(s) 259
SinI GGWCC 1 cut(s) 217
SmlI CTYRAG 2 cut(s) 751, 1337
SmoI CTYRAG 2 cut(s) 751, 1337
Sse9I AATT 8 cut(s) 56, 104, 473, 544, 615, 827, 944, 1598
SsiI CCGC 7 cut(s) 171, 220, 223, 256, 258, 1159, 1577
SspMI CTAG 4 cut(s) 1062, 1173, 1574, 1646
StyD4I CCNGG 3 cut(s) 132, 484, 895
StyI CCWWGG 1 cut(s) 672
TaaI ACNGT 2 cut(s) 882, 1241
TaiI ACGT 2 cut(s) 41, 352
TaqII GACCGA 1 cut(s) 680
TasI AATT 8 cut(s) 56, 104, 473, 544, 615, 827, 944, 1598
TatI WGTACW 1 cut(s) 732
TauI GCSGC 1 cut(s) 225
TfiI GAWTC 6 cut(s) 242, 342, 632, 776, 1146, 1189
Tru1I TTAA 4 cut(s) 752, 837, 1338, 1512
Tru9I TTAA 4 cut(s) 752, 837, 1338, 1512
TscAI CASTG 7 cut(s) 472, 582, 655, 887, 1160, 1392, 1480
TseFI GTSAC 1 cut(s) 650
TseI GCWGC 3 cut(s) 557, 1382, 1553
Tsp45I GTSAC 1 cut(s) 650
TspDTI ATGAA 6 cut(s) 752, 977, 1127, 1262, 1322, 1412
TspGWI ACGGA 2 cut(s) 781, 1252
TspRI CASTG 7 cut(s) 472, 582, 655, 887, 1160, 1392, 1480
Tth111I GACNNNGTC 1 cut(s) 919
Van91I CCANNNNNTGG 1 cut(s) 882
Vha464I CTTAAG 2 cut(s) 751, 1337
VpaK11BI GGWCC 1 cut(s) 217
XapI RAATTY 4 cut(s) 56, 473, 615, 827
XceI RCATGY 1 cut(s) 1490
XmnI GAANNNNTTC 1 cut(s) 827
XspI CTAG 4 cut(s) 1062, 1173, 1574, 1646
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.