Rroxscaffold_2G00088420

QWRF motif-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0010969
Physical Location & Seq
Reverse (-)
10359039 .. 10361488
2450 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00088420.1

Sequence Viewer

Length: 1713 bp
ATGGATCACCCTCACAAACCCAGAAAACCCAAATCCCGGGAAGTAAGCTCGCGCTTCCTATCTTGCTCTACACCCGAAACCTCCACACTCCCATCTCCAAGCCACCCTCTCTCGCCGGCCCGGCGAAAACTCTGTTCGCCGATATCCGATACCCGAAAACCCAAAACTCATCTCGACGATTCAAGCTCCATCCGAGGAGGAGGACTATGGCCATCTTCAACAACTAAGAACTTCGACTCTCTAGCAGTCCATCTCGGAATCGAGAGACTCAAAGATAGTGTCGACCGTAAAAACTCGACCTCCGTCACAACCCGGAGAGAAGCGAGTGTGTTTAATGAGAAAGACAACGCCAAAGAGAACCATAGACCGGTTCTCGGAGGGTCCGCGAGGTACACCGGGAAATTCAACTTTTCTGGGAAATCGTCGCCGTCGTCGTCTTCGACTTCAGTGTCTTCGAATAAACTATCGGCGGTGGTAGTTCCGGGGAGGTCTTCCGTTGACGGAAGTGGTCTGTATCAGAACTCAACTCGGCGAAGTTTCGATAGTTTCGCCGATGCTCTCGAGTCGGGATCGGAGTGCAGCGACGCCGGTTCCGGCAGGAATATTAATATCGGGTCTCCGAGTTTCGGGGCTCAGAGTTCGACGGCGAGTTCGAGAAAGTCGGGGGTGGAGGTTTCGTCTAAGTACATGAGTGATATTCAGACGAGGCATAATCGGAGATGGACGTCGGATTCTAATCTCCGGAATCCGATTTCGACAGAGAGCTCTCCGAAAATGAACAAGTTTAACCTGAAAAATGTGATCAAGAGGGCTAATTCTTTGACCGGGTCAAAGTTCGGGACGACGCAGTGGGCATTGTCGCCGGGAAGGAGCGCAGCGCAGCCTGTGTCGGCGGAGAGTTTGGGGAAGTCGACGTCATTTTCGAGCTTGAAGCCTCCGACTAGTCCTTCGAAAAGTAAGGGAGTGGAGAAGTTGCTCAATATGGGGTTCGACTTGTTCAAGAGTAAAAAATCTTCTTCATCTACTAGGTCACTGGTGAGGTCTAATTCCGGCTATGGTGTGACCGATACCGGTCATCAACTCCGGATGCTTCAGAACAGATTGATGCAATGGCGGTACGCAAATGCAAGAGCTGAAATTGTGAATGGCAATGTTGCTGATCAAGCACAGTTCAATTTTTTGAGTGCTTGTAATAGTCTTATGAAGTTGCGGCAATCCGTGCTGCATAAGAAACTGAAGCTTCAGAAAGAAAGGCTCGAAATGAAGCTGAACTTTATACTACATTCTCAATTTGAGCCATTGGAAGCTTGGGGAGATATGGAAAGGCAACATATGGGAGCAGTTTCTATGTTGAAAGAGAGCTTACATTCTGTTGTGTGCAGAGTACCTCTCACTGAAGGAGCAAAGGTGGATACTCAATCAGCTGCCATCAATCTTCGAAATGCTTCAGATTACTCAGCCTCCATTAAGTTGATATTGGCTACATTTGCCCCCTCGGCTGAGCAGACTAGTGTGTTGATATCAGAATTAGCAGAGGTGGTTGCGCAAGAAAAGTTGCTTTCTGATGAGTGTCTTGAGCTCTGTAGAACCATATCAGAACTAGAGATGGAAGAGAGGGGATTGAAGTGTAGTATTATTCAATTGGAATCGTGGAAACAAGAGCAAATTCAGCTGCAGCAGCAGCAATCAAGACAAGAAGATATCATGCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
QWRF

Protein Analysis

570

Amino Acids

62.63

Weight (kDa)

9.72

Isoelectric Point (pI)

59.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
QWRF PF04484 213 - 520 4.2e-76 QWRF family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 448
AatII GACGTC 2 cut(s) 728, 917
Acc16I TGCGCA 1 cut(s) 1545
AccI GTMKAC 2 cut(s) 282, 911
AccII CGCG 2 cut(s) 52, 386
AccIII TCCGGA 2 cut(s) 741, 1083
AciI CCGC 5 cut(s) 384, 470, 893, 1114, 1210
AclWI GGATC 2 cut(s) 12, 577
AcoI YGGCCR 1 cut(s) 209
AcsI RAATTY 2 cut(s) 401, 1665
AcuI CTGAAG 6 cut(s) 429, 1076, 1226, 1256, 1416, 1431
AcyI GRCGYC 3 cut(s) 585, 725, 914
AfaI GTAC 4 cut(s) 392, 686, 1118, 1386
AfiI CCNNNNNNNGG 4 cut(s) 36, 367, 374, 626
AgeI ACCGGT 2 cut(s) 367, 1070
AgsI TTSAA 9 cut(s) 183, 219, 406, 931, 1000, 1174, 1354, 1624, 1640
AhlI ACTAGT 2 cut(s) 941, 1508
Alw21I GWGCWC 2 cut(s) 767, 1581
Alw26I GTCTC 2 cut(s) 259, 621
AlwI GGATC 2 cut(s) 12, 577
Ama87I CYCGRG 2 cut(s) 36, 560
Aor13HI TCCGGA 2 cut(s) 741, 1083
AoxI GGCC 2 cut(s) 117, 209
ApeKI GCWGC 9 cut(s) 579, 875, 880, 1222, 1424, 1672, 1675, 1678, 1681
ApoI RAATTY 2 cut(s) 401, 1665
ArsI GACNNNNNNTTYG 8 cut(s) 264, 296, 634, 666, 904, 931, 936, 963
AseI ATTAAT 1 cut(s) 606
AsiGI ACCGGT 2 cut(s) 367, 1070
Asp700I GAANNNNTTC 1 cut(s) 1444
AspLEI GCGC 4 cut(s) 54, 875, 880, 1546
AspS9I GGNCC 2 cut(s) 118, 381
AsuC2I CCSGG 8 cut(s) 37, 38, 121, 313, 397, 483, 826, 864
AsuHPI GGTGA 1 cut(s) 1048
AsuII TTCGAA 3 cut(s) 455, 950, 1438
AvaI CYCGRG 2 cut(s) 36, 560
AvaII GGWCC 1 cut(s) 381
BalI TGGCCA 1 cut(s) 211
BanII GRGCYC 3 cut(s) 634, 767, 1581
BarI GAAGNNNNNNTAC 2 cut(s) 997, 1029
BbsI GAAGAC 3 cut(s) 429, 444, 483
Bbv12I GWGCWC 2 cut(s) 767, 1581
BbvI GCAGC 9 cut(s) 591, 887, 892, 1209, 1411, 1659, 1687, 1690, 1693
BccI CCATC 7 cut(s) 100, 197, 220, 258, 714, 1436, 1600
BceAI ACGGC 2 cut(s) 412, 660
BciVI GTATCC 1 cut(s) 1405
BclI TGATCA 2 cut(s) 801, 1159
BcnI CCSGG 8 cut(s) 37, 38, 121, 313, 397, 483, 826, 864
BcoDI GTCTC 2 cut(s) 259, 621
BcuI ACTAGT 2 cut(s) 941, 1508
BfaI CTAG 5 cut(s) 242, 942, 1026, 1509, 1601
BfmI CTRYAG 2 cut(s) 1582, 1673
BfuI GTATCC 1 cut(s) 1405
BglI GCCNNNNNGGC 2 cut(s) 121, 1496
BlpI GCTNAGC 1 cut(s) 1500
Bme1390I CCNGG 8 cut(s) 37, 38, 121, 313, 397, 483, 826, 864
Bme18I GGWCC 1 cut(s) 381
BmeT110I CYCGRG 2 cut(s) 36, 560
BmgT120I GGNCC 2 cut(s) 118, 381
BmiI GGNNCC 2 cut(s) 382, 592
BmrFI CCNGG 8 cut(s) 37, 38, 121, 313, 397, 483, 826, 864
BmsI GCATC 3 cut(s) 544, 1077, 1095
BoxI GACNNNNGTC 1 cut(s) 302
BpiI GAAGAC 3 cut(s) 429, 444, 483
BplI GAGNNNNNCTC 2 cut(s) 1374, 1406
Bpu1102I GCTNAGC 1 cut(s) 1500
Bpu14I TTCGAA 3 cut(s) 455, 950, 1438
BpuEI CTTGAG 1 cut(s) 1595
BpuMI CCSGG 8 cut(s) 37, 38, 121, 313, 397, 483, 826, 864
BsaBI GATNNNNATC 1 cut(s) 735
BsaHI GRCGYC 3 cut(s) 585, 725, 914
BsaI GGTCTC 1 cut(s) 621
BsaJI CCNNGG 4 cut(s) 36, 193, 482, 1494
BsaWI WCCGGW 4 cut(s) 367, 741, 1070, 1083
BsaXI ACNNNNNCTCC 8 cut(s) 284, 314, 662, 692, 1392, 1422, 1445, 1475
Bsc4I CCNNNNNNNGG 4 cut(s) 36, 367, 374, 626
Bse118I RCCGGY 4 cut(s) 115, 367, 587, 1070
Bse1I ACTGG 1 cut(s) 1038
Bse3DI GCAATG 2 cut(s) 1115, 1156
Bse8I GATNNNNATC 1 cut(s) 735
BseAI TCCGGA 2 cut(s) 741, 1083
BseDI CCNNGG 4 cut(s) 36, 193, 482, 1494
BseGI GGATG 2 cut(s) 189, 1092
BseJI GATNNNNATC 1 cut(s) 735
BseLI CCNNNNNNNGG 4 cut(s) 36, 367, 374, 626
BseMI GCAATG 2 cut(s) 1115, 1156
BseMII CTCAG 3 cut(s) 647, 1470, 1491
BseNI ACTGG 1 cut(s) 1038
BseRI GAGGAG 2 cut(s) 210, 213
BseXI GCAGC 9 cut(s) 591, 887, 892, 1209, 1411, 1659, 1687, 1690, 1693
BsgI GTGCAG 2 cut(s) 598, 1399
Bsh1236I CGCG 2 cut(s) 52, 386
Bsh1285I CGRYCG 1 cut(s) 286
BshFI GGCC 2 cut(s) 119, 211
BshTI ACCGGT 2 cut(s) 367, 1070
BsiEI CGRYCG 1 cut(s) 286
BsiHKAI GWGCWC 2 cut(s) 767, 1581
BsiHKCI CYCGRG 2 cut(s) 36, 560
BslFI GGGAC 1 cut(s) 853
BslI CCNNNNNNNGG 4 cut(s) 36, 367, 374, 626
BsmAI GTCTC 2 cut(s) 259, 621
BsmFI GGGAC 1 cut(s) 853
BsnI GGCC 2 cut(s) 119, 211
Bso31I GGTCTC 1 cut(s) 621
BsoBI CYCGRG 2 cut(s) 36, 560
Bsp119I TTCGAA 3 cut(s) 455, 950, 1438
Bsp1286I GDGCHC 3 cut(s) 634, 767, 1581
Bsp13I TCCGGA 2 cut(s) 741, 1083
Bsp143I GATC 4 cut(s) 4, 569, 801, 1159
Bsp1720I GCTNAGC 1 cut(s) 1500
BspACI CCGC 5 cut(s) 384, 470, 893, 1114, 1210
BspANI GGCC 2 cut(s) 119, 211
BspCNI CTCAG 3 cut(s) 646, 1469, 1492
BspEI TCCGGA 2 cut(s) 741, 1083
BspFNI CGCG 2 cut(s) 52, 386
BspLI GGNNCC 2 cut(s) 382, 592
BspMAI CTGCAG 1 cut(s) 1677
BspPI GGATC 2 cut(s) 12, 577
BspT104I TTCGAA 3 cut(s) 455, 950, 1438
BspTNI GGTCTC 1 cut(s) 621
BsrDI GCAATG 2 cut(s) 1115, 1156
BsrFI RCCGGY 4 cut(s) 115, 367, 587, 1070
BsrI ACTGG 1 cut(s) 1038
BssAI RCCGGY 4 cut(s) 115, 367, 587, 1070
BssECI CCNNGG 4 cut(s) 36, 193, 482, 1494
BssMI GATC 4 cut(s) 4, 569, 801, 1159
BssNI GRCGYC 3 cut(s) 585, 725, 914
Bst4CI ACNGT 2 cut(s) 287, 1170
Bst6I CTCTTC 1 cut(s) 1605
BstACI GRCGYC 3 cut(s) 585, 725, 914
BstAPI GCANNNNNTGC 1 cut(s) 1219
BstBI TTCGAA 3 cut(s) 455, 950, 1438
BstC8I GCNNGC 2 cut(s) 50, 117
BstDEI CTNAG 5 cut(s) 225, 633, 681, 1456, 1500
BstF5I GGATG 2 cut(s) 189, 1092
BstFNI CGCG 2 cut(s) 52, 386
BstHHI GCGC 4 cut(s) 54, 875, 880, 1546
BstKTI GATC 4 cut(s) 7, 572, 804, 1162
BstMAI GTCTC 2 cut(s) 259, 621
BstMBI GATC 4 cut(s) 4, 569, 801, 1159
BstMCI CGRYCG 1 cut(s) 286
BstMWI GCNNNNNNNGC 8 cut(s) 121, 1163, 1219, 1487, 1496, 1669, 1678, 1681
BstPAI GACNNNNGTC 1 cut(s) 302
BstSCI CCNGG 8 cut(s) 35, 36, 119, 311, 395, 481, 824, 862
BstSFI CTRYAG 2 cut(s) 1582, 1673
BstUI CGCG 2 cut(s) 52, 386
BstV1I GCAGC 9 cut(s) 591, 887, 892, 1209, 1411, 1659, 1687, 1690, 1693
BstV2I GAAGAC 3 cut(s) 429, 444, 483
BsuI GTATCC 1 cut(s) 1405
BsuRI GGCC 2 cut(s) 119, 211
BtsCI GGATG 2 cut(s) 189, 1092
BtsI GCAGTG 1 cut(s) 854
BtsIMutI CAGTG 4 cut(s) 453, 854, 1031, 1392
Cac8I GCNNGC 2 cut(s) 50, 117
CfoI GCGC 4 cut(s) 54, 875, 880, 1546
Cfr10I RCCGGY 4 cut(s) 115, 367, 587, 1070
Cfr13I GGNCC 2 cut(s) 118, 381
Cfr9I CCCGGG 1 cut(s) 36
CseI GACGC 2 cut(s) 593, 853
Csp6I GTAC 4 cut(s) 391, 685, 1117, 1385
CspAI ACCGGT 2 cut(s) 367, 1070
CviAII CATG 3 cut(s) 688, 1705, 1710
CviQI GTAC 4 cut(s) 391, 685, 1117, 1385
DdeI CTNAG 5 cut(s) 225, 633, 681, 1456, 1500
DpnI GATC 4 cut(s) 6, 571, 803, 1161
DpnII GATC 4 cut(s) 4, 569, 801, 1159
DrdI GACNNNNNNGTC 1 cut(s) 448
DseDI GACNNNNNNGTC 1 cut(s) 448
EaeI YGGCCR 1 cut(s) 209
Eam1104I CTCTTC 1 cut(s) 1605
EarI CTCTTC 1 cut(s) 1605
EciI GGCGGA 1 cut(s) 908
Ecl136II GAGCTC 2 cut(s) 765, 1579
Eco24I GRGCYC 3 cut(s) 634, 767, 1581
Eco31I GGTCTC 1 cut(s) 621
Eco32I GATATC 3 cut(s) 144, 1521, 1702
Eco47I GGWCC 1 cut(s) 381
Eco53kI GAGCTC 2 cut(s) 765, 1579
Eco57I CTGAAG 6 cut(s) 429, 1076, 1226, 1256, 1416, 1431
Eco88I CYCGRG 2 cut(s) 36, 560
EcoICRI GAGCTC 2 cut(s) 765, 1579
EcoRV GATATC 3 cut(s) 144, 1521, 1702
EcoT38I GRGCYC 3 cut(s) 634, 767, 1581
FaeI CATG 3 cut(s) 691, 1708, 1713
FalI AAGNNNNNCTT 2 cut(s) 1256, 1288
FaqI GGGAC 1 cut(s) 853
FatI CATG 3 cut(s) 687, 1704, 1709
FauNDI CATATG 1 cut(s) 1332
FbaI TGATCA 2 cut(s) 801, 1159
FblI GTMKAC 2 cut(s) 282, 911
FokI GGATG 2 cut(s) 176, 1099
FriOI GRGCYC 3 cut(s) 634, 767, 1581
FspBI CTAG 5 cut(s) 242, 942, 1026, 1509, 1601
FspI TGCGCA 1 cut(s) 1545
GlaI GCGC 4 cut(s) 53, 874, 879, 1545
HaeIII GGCC 2 cut(s) 119, 211
HgaI GACGC 2 cut(s) 593, 853
HhaI GCGC 4 cut(s) 54, 875, 880, 1546
Hin1I GRCGYC 3 cut(s) 585, 725, 914
Hin1II CATG 3 cut(s) 691, 1708, 1713
Hin6I GCGC 4 cut(s) 52, 873, 878, 1544
HinP1I GCGC 4 cut(s) 52, 873, 878, 1544
HincII GTYRAC 3 cut(s) 283, 499, 912
HindII GTYRAC 3 cut(s) 283, 499, 912
HindIII AAGCTT 2 cut(s) 1238, 1305
HinfI GANTC 8 cut(s) 179, 236, 258, 267, 563, 731, 745, 1646
HphI GGTGA 1 cut(s) 1048
Hpy166II GTNNAC 4 cut(s) 283, 393, 499, 912
Hpy8I GTNNAC 4 cut(s) 283, 393, 499, 912
Hpy99I CGWCG 9 cut(s) 179, 427, 433, 436, 587, 646, 730, 847, 916
HpyAV CCTTC 3 cut(s) 861, 957, 1391
HpyCH4III ACNGT 2 cut(s) 287, 1170
HpyCH4IV ACGT 2 cut(s) 725, 914
HpyCH4V TGCA 6 cut(s) 579, 1108, 1127, 1225, 1380, 1675
HpyF10VI GCNNNNNNNGC 8 cut(s) 121, 1163, 1219, 1487, 1496, 1669, 1678, 1681
HpyF3I CTNAG 5 cut(s) 225, 633, 681, 1456, 1500
HpySE526I ACGT 2 cut(s) 725, 914
Hsp92I GRCGYC 3 cut(s) 585, 725, 914
Hsp92II CATG 3 cut(s) 691, 1708, 1713
HspAI GCGC 4 cut(s) 52, 873, 878, 1544
Kpn2I TCCGGA 2 cut(s) 741, 1083
KroI GCCGGC 1 cut(s) 115
KroNI GCCGGC 1 cut(s) 117
Ksp22I TGATCA 2 cut(s) 801, 1159
Kzo9I GATC 4 cut(s) 4, 569, 801, 1159
LmnI GCTCC 4 cut(s) 191, 870, 1337, 1400
Lsp1109I GCAGC 9 cut(s) 591, 887, 892, 1209, 1411, 1659, 1687, 1690, 1693
LweI GCATC 3 cut(s) 544, 1077, 1095
MaeI CTAG 5 cut(s) 242, 942, 1026, 1509, 1601
MaeII ACGT 2 cut(s) 725, 914
MaeIII GTNAC 3 cut(s) 304, 1029, 1060
MalI GATC 4 cut(s) 6, 571, 803, 1161
MboI GATC 4 cut(s) 4, 569, 801, 1159
MboII GAAGA 9 cut(s) 207, 429, 444, 483, 1005, 1008, 1427, 1622, 1709
MfeI CAATTG 1 cut(s) 1640
MhlI GDGCHC 3 cut(s) 634, 767, 1581
MlsI TGGCCA 1 cut(s) 211
MluCI AATT 9 cut(s) 401, 814, 1045, 1137, 1174, 1289, 1526, 1640, 1665
MluNI TGGCCA 1 cut(s) 211
MlyI GAGTC 3 cut(s) 230, 261, 572
MmeI TCCRAC 2 cut(s) 708, 962
Mox20I TGGCCA 1 cut(s) 211
MroI TCCGGA 2 cut(s) 741, 1083
MroNI GCCGGC 1 cut(s) 115
MroXI GAANNNNTTC 1 cut(s) 1444
MscI TGGCCA 1 cut(s) 211
MseI TTAA 4 cut(s) 333, 606, 786, 1467
Msp20I TGGCCA 1 cut(s) 211
MspA1I CMGCKG 2 cut(s) 1424, 1672
MspR9I CCNGG 8 cut(s) 37, 38, 121, 313, 397, 483, 826, 864
MteI GCGCNGCGC 1 cut(s) 876
MunI CAATTG 1 cut(s) 1640
MvnI CGCG 2 cut(s) 52, 386
MwoI GCNNNNNNNGC 8 cut(s) 121, 1163, 1219, 1487, 1496, 1669, 1678, 1681
NaeI GCCGGC 1 cut(s) 117
NciI CCSGG 8 cut(s) 37, 38, 121, 313, 397, 483, 826, 864
NdeI CATATG 1 cut(s) 1332
NdeII GATC 4 cut(s) 4, 569, 801, 1159
NgoMIV GCCGGC 1 cut(s) 115
NlaIII CATG 3 cut(s) 691, 1708, 1713
NlaIV GGNNCC 2 cut(s) 382, 592
NmeAIII GCCGAG 2 cut(s) 508, 1475
NmuCI GTSAC 3 cut(s) 304, 1029, 1060
NsbI TGCGCA 1 cut(s) 1545
NspV TTCGAA 3 cut(s) 455, 950, 1438
PaeR7I CTCGAG 1 cut(s) 560
PcsI WCGNNNNNNNCGW 5 cut(s) 428, 431, 437, 650, 920
PdiI GCCGGC 1 cut(s) 117
PdmI GAANNNNTTC 1 cut(s) 1444
PfeI GAWTC 5 cut(s) 179, 258, 731, 745, 1646
PflFI GACNNNGTC 1 cut(s) 826
PinAI ACCGGT 2 cut(s) 367, 1070
PleI GAGTC 3 cut(s) 230, 261, 571
PpsI GAGTC 3 cut(s) 230, 261, 571
PshAI GACNNNNGTC 1 cut(s) 302
PshBI ATTAAT 1 cut(s) 606
Psp124BI GAGCTC 2 cut(s) 767, 1581
PspN4I GGNNCC 2 cut(s) 382, 592
PspPI GGNCC 2 cut(s) 118, 381
PstI CTGCAG 1 cut(s) 1677
PsyI GACNNNGTC 1 cut(s) 826
PvuII CAGCTG 2 cut(s) 1424, 1672
RsaI GTAC 4 cut(s) 392, 686, 1118, 1386
RsaNI GTAC 4 cut(s) 391, 685, 1117, 1385
SacI GAGCTC 2 cut(s) 767, 1581
SalI GTCGAC 2 cut(s) 281, 910
SaqAI TTAA 4 cut(s) 333, 606, 786, 1467
Sau3AI GATC 4 cut(s) 4, 569, 801, 1159
Sau96I GGNCC 2 cut(s) 118, 381
SchI GAGTC 3 cut(s) 230, 261, 572
ScrFI CCNGG 8 cut(s) 37, 38, 121, 313, 397, 483, 826, 864
SduI GDGCHC 3 cut(s) 634, 767, 1581
SfaNI GCATC 3 cut(s) 544, 1077, 1095
SfcI CTRYAG 2 cut(s) 1582, 1673
Sfr274I CTCGAG 1 cut(s) 560
SfuI TTCGAA 3 cut(s) 455, 950, 1438
SinI GGWCC 1 cut(s) 381
SlaI CTCGAG 1 cut(s) 560
SmaI CCCGGG 1 cut(s) 38
SmlI CTYRAG 2 cut(s) 560, 1574
SmoI CTYRAG 2 cut(s) 560, 1574
SpeI ACTAGT 2 cut(s) 941, 1508
Sse9I AATT 9 cut(s) 401, 814, 1045, 1137, 1174, 1289, 1526, 1640, 1665
SsiI CCGC 5 cut(s) 384, 470, 893, 1114, 1210
SspI AATATT 1 cut(s) 604
SspMI CTAG 5 cut(s) 242, 942, 1026, 1509, 1601
SstI GAGCTC 2 cut(s) 767, 1581
StyD4I CCNGG 8 cut(s) 35, 36, 119, 311, 395, 481, 824, 862
TaaI ACNGT 2 cut(s) 287, 1170
TaiI ACGT 2 cut(s) 728, 917
TaqII GACCGA 1 cut(s) 1079
TasI AATT 9 cut(s) 401, 814, 1045, 1137, 1174, 1289, 1526, 1640, 1665
TatI WGTACW 1 cut(s) 684
TauI GCSGC 1 cut(s) 1213
TfiI GAWTC 5 cut(s) 179, 258, 731, 745, 1646
Tru1I TTAA 4 cut(s) 333, 606, 786, 1467
Tru9I TTAA 4 cut(s) 333, 606, 786, 1467
TscAI CASTG 4 cut(s) 453, 854, 1038, 1399
TseFI GTSAC 3 cut(s) 304, 1029, 1060
TseI GCWGC 9 cut(s) 579, 875, 880, 1222, 1424, 1672, 1675, 1678, 1681
Tsp45I GTSAC 3 cut(s) 304, 1029, 1060
TspDTI ATGAA 4 cut(s) 791, 1008, 1217, 1277
TspGWI ACGGA 4 cut(s) 292, 484, 516, 1207
TspMI CCCGGG 1 cut(s) 36
TspRI CASTG 4 cut(s) 453, 854, 1038, 1399
Tth111I GACNNNGTC 1 cut(s) 826
VpaK11BI GGWCC 1 cut(s) 381
VspI ATTAAT 1 cut(s) 606
XapI RAATTY 2 cut(s) 401, 1665
XcmI CCANNNNNNNNNTGG 1 cut(s) 1305
XhoI CTCGAG 1 cut(s) 560
XmaI CCCGGG 1 cut(s) 36
XmiI GTMKAC 2 cut(s) 282, 911
XmnI GAANNNNTTC 1 cut(s) 1444
XspI CTAG 5 cut(s) 242, 942, 1026, 1509, 1601
ZraI GACGTC 2 cut(s) 726, 915
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.