RchiOBHm_Chr2g0161991

QWRF motif-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0010969
Physical Location & Seq
Forward (+)
77796314 .. 77798314
2001 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ53030

Sequence Viewer

Length: 1557 bp
ATGGATCACCCTCACAAACCCAGAAAGCCCAAATCCCGGGAAGTAAGCTCCCGCTTCCTATCTTCCCCCGCACCCGAAACCTCCACACTCCCATCTCCAAGCCACCCTCTCTCGCCGGCCCGGAGAAAACTCGGTTCGCCGATATCCGATGCCCGCAAACCCAAAACTCATCTCGACGATTCAAGCTCCATCCGAGGAGGAGGACTATGGCCATCTTCAACAACCAAGAACTTCGACTCTCTAGCAGTCCATCTCGGAATCGAGAGACTCAAAGACAGTACCGACCGTAAAAACTCGACCTCCGTCACAAGCCGGAGAGAAGCGAGTGTGTTTAATGAGAAAGACAACGCCAAAGAGAACCATAGACCGGTTCTCGGAGGGTCGGCGAGGTACACCGGGAAATTCAAATTTTCCGGGAAATCGTCGCCGTCGCCGTCGTCGTCTTCGACTTCAGTGTCTTCGAATAAACTATCGGCGGTGGTAGTTCCGGGGAGATTTTCCGTTGACGGAAGTGGTCTGTATCAGAACTCAACTCGGCGAAGTTTCGACAGTTTCGCCGATACTCTCGAGTCGGGATCGGAGTGCAGCGACGCCGGTTCAGGCAGGAATATTAATATCGGGTCGCCGAGTTTCGGGGCTCAGAGCTCGACGGCGAGTTCGAGAAAGTCGGGGGTGGAGGTTTCGTCTAAGTACATGAGTGATATTCAGACGAGGCATCATCGGAGATGGACGTCGGATTCTAATCTCCGGAATCCGATTTCGGCAGAGAGCTCTCCGAAAATGAACAAGTTTAACCTGAAAAATGTGATCAAGAGGGCTAATTCGTTGACCGGGTCAAAGTTCGGGACGACGCAGTGGGCATTGTCGCCGGGAAGGAGCGTGGCGCAGCCGGGGTCGGCGGAGAGTTTGGGGAAGTCGACGTCATTTTCGAGCTTGAAGCCTCCGACTAGTCCTTCGAAAAGTAAGGGAGTCGAGAAGTTGCTCAATATGGGGTTCGACTTGTTCAAGAGTAAAAAATCTTCATCATCTACTAGGTCACTGGTGAGGTCTAATTCCGGGTATGGTGTGACCGATACCGGTCATCAACTCCGGATGCTTCAGAACAGATTGATGCAATGGCGGTACGCAAATGCAAGAGTTGAAATTGTGAACGGCAATGTTGCTGATCAAGCACAGGTCAATTTTTTGAGTGCTTGTAATAGTCTTATGAAGTTGCGGCAATCCGTGCTGCATAAGAAACTGAAGCTTCAGAAAGAAAGGCTCGAAATGAAGCTGAACTTTACACTACATTCTCAATTTGAGCCATTGGAAGCTTGGGGAGATATGGAAAGGCAACATATGGGAGCAGTTTCTATGTTGAAAGAGAGCTTACATTCTGTTGTGTGCAGAGTGCCTCTAACTGAAGGTGCAAAGGCTGAGCAGACTAGTGTGTTGCTCTCAGAATTAGCAGAGGTTGTTGCGCAAGAAAAGTTGCTTTCTGATGAGTGTCTTGAGCTCTGTAGAACCATATCAGAACTAGAGGTAAGCTTTATTAGTTTTTTTTTCCAACATATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
QWRF

Protein Analysis

518

Amino Acids

56.74

Weight (kDa)

9.97

Isoelectric Point (pI)

56.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
QWRF PF04484 215 - 476 5.2e-60 QWRF family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 454
AatII GACGTC 2 cut(s) 734, 923
Acc16I TGCGCA 1 cut(s) 1461
AccI GTMKAC 1 cut(s) 917
AccIII TCCGGA 2 cut(s) 747, 1089
AciI CCGC 7 cut(s) 52, 69, 154, 476, 899, 1120, 1216
AclWI GGATC 2 cut(s) 12, 583
AcoI YGGCCR 1 cut(s) 209
AcsI RAATTY 2 cut(s) 401, 407
AcuI CTGAAG 5 cut(s) 435, 1082, 1232, 1262, 1422
AcyI GRCGYC 3 cut(s) 591, 731, 920
AfaI GTAC 4 cut(s) 280, 392, 692, 1124
AfiI CCNNNNNNNGG 4 cut(s) 36, 367, 374, 632
AgeI ACCGGT 2 cut(s) 367, 1076
AgsI TTSAA 7 cut(s) 183, 219, 406, 937, 1006, 1142, 1360
AhlI ACTAGT 2 cut(s) 947, 1424
Alw21I GWGCWC 3 cut(s) 647, 773, 1497
Alw26I GTCTC 1 cut(s) 259
AlwI GGATC 2 cut(s) 12, 583
Ama87I CYCGRG 2 cut(s) 36, 566
Aor13HI TCCGGA 2 cut(s) 747, 1089
AoxI GGCC 2 cut(s) 117, 209
ApeKI GCWGC 3 cut(s) 585, 886, 1228
ApoI RAATTY 2 cut(s) 401, 407
ArsI GACNNNNNNTTYG 6 cut(s) 640, 672, 910, 937, 942, 969
AseI ATTAAT 1 cut(s) 612
AsiGI ACCGGT 2 cut(s) 367, 1076
AspLEI GCGC 2 cut(s) 886, 1462
AspS9I GGNCC 1 cut(s) 118
AsuHPI GGTGA 1 cut(s) 1054
AsuII TTCGAA 2 cut(s) 461, 956
AvaI CYCGRG 2 cut(s) 36, 566
BalI TGGCCA 1 cut(s) 211
BanII GRGCYC 4 cut(s) 640, 647, 773, 1497
BarI GAAGNNNNNNTAC 2 cut(s) 1003, 1035
BbsI GAAGAC 2 cut(s) 435, 450
Bbv12I GWGCWC 3 cut(s) 647, 773, 1497
BbvI GCAGC 3 cut(s) 597, 898, 1215
BccI CCATC 5 cut(s) 100, 197, 220, 258, 720
BceAI ACGGC 4 cut(s) 412, 418, 666, 1168
BclI TGATCA 2 cut(s) 807, 1165
BcoDI GTCTC 1 cut(s) 259
BcuI ACTAGT 2 cut(s) 947, 1424
BfaI CTAG 5 cut(s) 242, 948, 1032, 1425, 1517
BfmI CTRYAG 1 cut(s) 1498
BisI GCNGC 4 cut(s) 586, 887, 1217, 1229
BlpI GCTNAGC 1 cut(s) 1416
BlsI GCNGC 4 cut(s) 587, 888, 1218, 1230
BmeT110I CYCGRG 2 cut(s) 36, 566
BmgT120I GGNCC 1 cut(s) 118
BmsI GCATC 4 cut(s) 139, 724, 1083, 1101
BoxI GACNNNNGTC 1 cut(s) 302
BpiI GAAGAC 2 cut(s) 435, 450
Bpu1102I GCTNAGC 1 cut(s) 1416
Bpu14I TTCGAA 2 cut(s) 461, 956
BpuEI CTTGAG 1 cut(s) 1511
BsaBI GATNNNNATC 1 cut(s) 741
BsaHI GRCGYC 3 cut(s) 591, 731, 920
BsaJI CCNNGG 4 cut(s) 36, 193, 488, 890
BsaWI WCCGGW 4 cut(s) 367, 747, 1076, 1089
BsaXI ACNNNNNCTCC 4 cut(s) 284, 314, 668, 698
Bsc4I CCNNNNNNNGG 4 cut(s) 36, 367, 374, 632
Bse118I RCCGGY 4 cut(s) 115, 367, 593, 1076
Bse1I ACTGG 1 cut(s) 1044
Bse3DI GCAATG 2 cut(s) 1121, 1162
Bse8I GATNNNNATC 1 cut(s) 741
BseAI TCCGGA 2 cut(s) 747, 1089
BseDI CCNNGG 4 cut(s) 36, 193, 488, 890
BseGI GGATG 2 cut(s) 189, 1098
BseJI GATNNNNATC 1 cut(s) 741
BseLI CCNNNNNNNGG 4 cut(s) 36, 367, 374, 632
BseMI GCAATG 2 cut(s) 1121, 1162
BseMII CTCAG 3 cut(s) 653, 1407, 1452
BseNI ACTGG 1 cut(s) 1044
BseRI GAGGAG 2 cut(s) 210, 213
BseXI GCAGC 3 cut(s) 597, 898, 1215
BsgI GTGCAG 2 cut(s) 604, 1405
Bsh1285I CGRYCG 1 cut(s) 286
BshFI GGCC 2 cut(s) 119, 211
BshTI ACCGGT 2 cut(s) 367, 1076
BsiEI CGRYCG 1 cut(s) 286
BsiHKAI GWGCWC 3 cut(s) 647, 773, 1497
BsiHKCI CYCGRG 2 cut(s) 36, 566
BslFI GGGAC 1 cut(s) 859
BslI CCNNNNNNNGG 4 cut(s) 36, 367, 374, 632
BsmAI GTCTC 1 cut(s) 259
BsmFI GGGAC 1 cut(s) 859
BsnI GGCC 2 cut(s) 119, 211
BsoBI CYCGRG 2 cut(s) 36, 566
Bsp119I TTCGAA 2 cut(s) 461, 956
Bsp1286I GDGCHC 4 cut(s) 640, 647, 773, 1497
Bsp13I TCCGGA 2 cut(s) 747, 1089
Bsp143I GATC 4 cut(s) 4, 575, 807, 1165
Bsp1720I GCTNAGC 1 cut(s) 1416
BspACI CCGC 7 cut(s) 52, 69, 154, 476, 899, 1120, 1216
BspANI GGCC 2 cut(s) 119, 211
BspCNI CTCAG 3 cut(s) 652, 1408, 1451
BspEI TCCGGA 2 cut(s) 747, 1089
BspPI GGATC 2 cut(s) 12, 583
BspT104I TTCGAA 2 cut(s) 461, 956
BsrDI GCAATG 2 cut(s) 1121, 1162
BsrFI RCCGGY 4 cut(s) 115, 367, 593, 1076
BsrI ACTGG 1 cut(s) 1044
BssAI RCCGGY 4 cut(s) 115, 367, 593, 1076
BssECI CCNNGG 4 cut(s) 36, 193, 488, 890
BssMI GATC 4 cut(s) 4, 575, 807, 1165
BssNI GRCGYC 3 cut(s) 591, 731, 920
Bst4CI ACNGT 3 cut(s) 278, 287, 551
BstACI GRCGYC 3 cut(s) 591, 731, 920
BstAPI GCANNNNNTGC 1 cut(s) 1225
BstBI TTCGAA 2 cut(s) 461, 956
BstC8I GCNNGC 2 cut(s) 117, 154
BstDEI CTNAG 4 cut(s) 639, 687, 1416, 1438
BstF5I GGATG 2 cut(s) 189, 1098
BstHHI GCGC 2 cut(s) 886, 1462
BstKTI GATC 4 cut(s) 7, 578, 810, 1168
BstMAI GTCTC 1 cut(s) 259
BstMBI GATC 4 cut(s) 4, 575, 807, 1165
BstMCI CGRYCG 1 cut(s) 286
BstMWI GCNNNNNNNGC 2 cut(s) 1169, 1225
BstPAI GACNNNNGTC 1 cut(s) 302
BstSFI CTRYAG 1 cut(s) 1498
BstV1I GCAGC 3 cut(s) 597, 898, 1215
BstV2I GAAGAC 2 cut(s) 435, 450
BsuRI GGCC 2 cut(s) 119, 211
BtsCI GGATG 2 cut(s) 189, 1098
BtsI GCAGTG 1 cut(s) 860
BtsIMutI CAGTG 3 cut(s) 459, 860, 1037
Cac8I GCNNGC 2 cut(s) 117, 154
CfoI GCGC 2 cut(s) 886, 1462
Cfr10I RCCGGY 4 cut(s) 115, 367, 593, 1076
Cfr13I GGNCC 1 cut(s) 118
Cfr9I CCCGGG 1 cut(s) 36
CseI GACGC 2 cut(s) 599, 859
Csp6I GTAC 4 cut(s) 279, 391, 691, 1123
CspAI ACCGGT 2 cut(s) 367, 1076
CviAII CATG 1 cut(s) 694
CviQI GTAC 4 cut(s) 279, 391, 691, 1123
DdeI CTNAG 4 cut(s) 639, 687, 1416, 1438
DpnI GATC 4 cut(s) 6, 577, 809, 1167
DpnII GATC 4 cut(s) 4, 575, 807, 1165
DrdI GACNNNNNNGTC 1 cut(s) 454
DseDI GACNNNNNNGTC 1 cut(s) 454
EaeI YGGCCR 1 cut(s) 209
EciI GGCGGA 1 cut(s) 914
Ecl136II GAGCTC 3 cut(s) 645, 771, 1495
Eco24I GRGCYC 4 cut(s) 640, 647, 773, 1497
Eco32I GATATC 1 cut(s) 144
Eco53kI GAGCTC 3 cut(s) 645, 771, 1495
Eco57I CTGAAG 5 cut(s) 435, 1082, 1232, 1262, 1422
Eco88I CYCGRG 2 cut(s) 36, 566
EcoICRI GAGCTC 3 cut(s) 645, 771, 1495
EcoRV GATATC 1 cut(s) 144
EcoT38I GRGCYC 4 cut(s) 640, 647, 773, 1497
FaeI CATG 1 cut(s) 697
FalI AAGNNNNNCTT 2 cut(s) 1262, 1294
FaqI GGGAC 1 cut(s) 859
FatI CATG 1 cut(s) 693
FauI CCCGC 3 cut(s) 59, 76, 161
FauNDI CATATG 1 cut(s) 1338
FbaI TGATCA 2 cut(s) 807, 1165
FblI GTMKAC 1 cut(s) 917
Fnu4HI GCNGC 4 cut(s) 586, 887, 1217, 1229
FokI GGATG 2 cut(s) 176, 1105
FriOI GRGCYC 4 cut(s) 640, 647, 773, 1497
Fsp4HI GCNGC 4 cut(s) 586, 887, 1217, 1229
FspBI CTAG 5 cut(s) 242, 948, 1032, 1425, 1517
FspI TGCGCA 1 cut(s) 1461
GlaI GCGC 2 cut(s) 885, 1461
GluI GCNGC 4 cut(s) 586, 887, 1217, 1229
HaeIII GGCC 2 cut(s) 119, 211
HgaI GACGC 2 cut(s) 599, 859
HhaI GCGC 2 cut(s) 886, 1462
Hin1I GRCGYC 3 cut(s) 591, 731, 920
Hin1II CATG 1 cut(s) 697
Hin6I GCGC 2 cut(s) 884, 1460
HinP1I GCGC 2 cut(s) 884, 1460
HincII GTYRAC 3 cut(s) 505, 828, 918
HindII GTYRAC 3 cut(s) 505, 828, 918
HindIII AAGCTT 3 cut(s) 1244, 1311, 1525
HinfI GANTC 8 cut(s) 179, 236, 258, 267, 569, 737, 751, 969
HphI GGTGA 1 cut(s) 1054
Hpy166II GTNNAC 5 cut(s) 393, 505, 828, 918, 1150
Hpy8I GTNNAC 5 cut(s) 393, 505, 828, 918, 1150
HpyAV CCTTC 3 cut(s) 867, 963, 1397
HpyCH4III ACNGT 3 cut(s) 278, 287, 551
HpyCH4IV ACGT 2 cut(s) 731, 920
HpyCH4V TGCA 6 cut(s) 585, 1114, 1133, 1231, 1386, 1409
HpyF10VI GCNNNNNNNGC 2 cut(s) 1169, 1225
HpyF3I CTNAG 4 cut(s) 639, 687, 1416, 1438
HpySE526I ACGT 2 cut(s) 731, 920
Hsp92I GRCGYC 3 cut(s) 591, 731, 920
Hsp92II CATG 1 cut(s) 697
HspAI GCGC 2 cut(s) 884, 1460
Kpn2I TCCGGA 2 cut(s) 747, 1089
KroI GCCGGC 1 cut(s) 115
KroNI GCCGGC 1 cut(s) 117
Ksp22I TGATCA 2 cut(s) 807, 1165
Kzo9I GATC 4 cut(s) 4, 575, 807, 1165
LmnI GCTCC 4 cut(s) 53, 191, 876, 1343
Lsp1109I GCAGC 3 cut(s) 597, 898, 1215
LweI GCATC 4 cut(s) 139, 724, 1083, 1101
MaeI CTAG 5 cut(s) 242, 948, 1032, 1425, 1517
MaeII ACGT 2 cut(s) 731, 920
MaeIII GTNAC 3 cut(s) 304, 1035, 1066
MalI GATC 4 cut(s) 6, 577, 809, 1167
MboI GATC 4 cut(s) 4, 575, 807, 1165
MboII GAAGA 5 cut(s) 54, 207, 435, 450, 1011
MhlI GDGCHC 4 cut(s) 640, 647, 773, 1497
MlsI TGGCCA 1 cut(s) 211
MluCI AATT 8 cut(s) 401, 407, 820, 1051, 1143, 1180, 1295, 1442
MluNI TGGCCA 1 cut(s) 211
MlyI GAGTC 4 cut(s) 230, 261, 578, 978
MmeI TCCRAC 2 cut(s) 714, 968
Mox20I TGGCCA 1 cut(s) 211
MroI TCCGGA 2 cut(s) 747, 1089
MroNI GCCGGC 1 cut(s) 115
MscI TGGCCA 1 cut(s) 211
MseI TTAA 3 cut(s) 333, 612, 792
Msp20I TGGCCA 1 cut(s) 211
MwoI GCNNNNNNNGC 2 cut(s) 1169, 1225
NaeI GCCGGC 1 cut(s) 117
NdeI CATATG 1 cut(s) 1338
NdeII GATC 4 cut(s) 4, 575, 807, 1165
NgoMIV GCCGGC 1 cut(s) 115
NlaIII CATG 1 cut(s) 697
NmeAIII GCCGAG 2 cut(s) 514, 651
NmuCI GTSAC 3 cut(s) 304, 1035, 1066
NsbI TGCGCA 1 cut(s) 1461
NspV TTCGAA 2 cut(s) 461, 956
PaeR7I CTCGAG 1 cut(s) 566
PcsI WCGNNNNNNNCGW 5 cut(s) 431, 437, 443, 656, 926
PdiI GCCGGC 1 cut(s) 117
PfeI GAWTC 4 cut(s) 179, 258, 737, 751
PflFI GACNNNGTC 1 cut(s) 832
PfoI TCCNGGA 1 cut(s) 413
PinAI ACCGGT 2 cut(s) 367, 1076
PkrI GCNGC 4 cut(s) 587, 888, 1218, 1230
PleI GAGTC 4 cut(s) 230, 261, 577, 977
PpsI GAGTC 4 cut(s) 230, 261, 577, 977
PshAI GACNNNNGTC 1 cut(s) 302
PshBI ATTAAT 1 cut(s) 612
Psp124BI GAGCTC 3 cut(s) 647, 773, 1497
PspPI GGNCC 1 cut(s) 118
PsyI GACNNNGTC 1 cut(s) 832
RsaI GTAC 4 cut(s) 280, 392, 692, 1124
RsaNI GTAC 4 cut(s) 279, 391, 691, 1123
SacI GAGCTC 3 cut(s) 647, 773, 1497
SalI GTCGAC 1 cut(s) 916
SaqAI TTAA 3 cut(s) 333, 612, 792
SatI GCNGC 4 cut(s) 586, 887, 1217, 1229
Sau3AI GATC 4 cut(s) 4, 575, 807, 1165
Sau96I GGNCC 1 cut(s) 118
SchI GAGTC 4 cut(s) 230, 261, 578, 978
SduI GDGCHC 4 cut(s) 640, 647, 773, 1497
SfaNI GCATC 4 cut(s) 139, 724, 1083, 1101
SfcI CTRYAG 1 cut(s) 1498
Sfr274I CTCGAG 1 cut(s) 566
SfuI TTCGAA 2 cut(s) 461, 956
SlaI CTCGAG 1 cut(s) 566
SmaI CCCGGG 1 cut(s) 38
SmlI CTYRAG 2 cut(s) 566, 1490
SmoI CTYRAG 2 cut(s) 566, 1490
SpeI ACTAGT 2 cut(s) 947, 1424
Sse9I AATT 8 cut(s) 401, 407, 820, 1051, 1143, 1180, 1295, 1442
SsiI CCGC 7 cut(s) 52, 69, 154, 476, 899, 1120, 1216
SspI AATATT 1 cut(s) 610
SspMI CTAG 5 cut(s) 242, 948, 1032, 1425, 1517
SstI GAGCTC 3 cut(s) 647, 773, 1497
TaaI ACNGT 3 cut(s) 278, 287, 551
TaiI ACGT 2 cut(s) 734, 923
TaqII GACCGA 1 cut(s) 1085
TasI AATT 8 cut(s) 401, 407, 820, 1051, 1143, 1180, 1295, 1442
TatI WGTACW 1 cut(s) 690
TauI GCSGC 1 cut(s) 1219
TfiI GAWTC 4 cut(s) 179, 258, 737, 751
Tru1I TTAA 3 cut(s) 333, 612, 792
Tru9I TTAA 3 cut(s) 333, 612, 792
TscAI CASTG 3 cut(s) 459, 860, 1044
TseFI GTSAC 3 cut(s) 304, 1035, 1066
TseI GCWGC 3 cut(s) 585, 886, 1228
Tsp45I GTSAC 3 cut(s) 304, 1035, 1066
TspDTI ATGAA 4 cut(s) 797, 1011, 1223, 1283
TspGWI ACGGA 4 cut(s) 292, 490, 522, 1213
TspMI CCCGGG 1 cut(s) 36
TspRI CASTG 3 cut(s) 459, 860, 1044
Tth111I GACNNNGTC 1 cut(s) 832
VspI ATTAAT 1 cut(s) 612
XapI RAATTY 2 cut(s) 401, 407
XcmI CCANNNNNNNNNTGG 1 cut(s) 1311
XhoI CTCGAG 1 cut(s) 566
XmaI CCCGGG 1 cut(s) 36
XmiI GTMKAC 1 cut(s) 917
XspI CTAG 5 cut(s) 242, 948, 1032, 1425, 1517
ZraI GACGTC 2 cut(s) 732, 921
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.