AT2G25620

protein phosphatase 2C 22

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0008007
Physical Location & Seq
Reverse (-)
10902716 .. 10905287
2572 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G25620.1

Sequence Viewer

Length: 1179 bp
ATGGAAGAAACTAGAGGAATTTCTGATCCAGAGAATGGGAGTTCGAGTTACGGCGGTAAACCGCCGAATCCACTCTCCTTCTCTTCTTCCTCCGCCGCTGCTGCCGTTTACAGGCAAACCTTCGACGGCGAGCGATCGTTGGCGCCGTGTAATAAGAGGTCACTGGTTCGACACTCATCTCTCGTGAAGACAATGGTGTCAGATATATCTGTTGAGAATGAGTTTACTATAGAGAAGAACAAGTCTGAGTTTGTTCCTGCTACGCGTTCTGGAGCTTGGTCTGATATTGGCTCCAGGTCAAGCATGGAAGATGCTTATCTATGCGTTGATAATTTCATGGATAGCTTTGGCCTTCTGAATTCTGAGGCTGGTCCAAGTGCCTTCTATGGGGTATTTGATGGACATGGTGGGAAGCATGCCGCTGAGTTTGCATGTCACCATATACCGAGGTACATTGTTGAAGATCAAGAGTTTCCTAGTGAAATCAATAAGGTGTTATCTTCAGCATTTCTTCAAACAGACACTGCCTTCTTAGAGGCGTGTTCATTGGATGGGAGCCTTGCTTCAGGAACTACTGCTTTGGCAGCTATTCTTTTTGGAAGGTCGTTGGTGGTAGCAAACGCTGGAGATTGCAGAGCAGTCTTATCCCGTCAGGGAAAAGCCATTGAAATGTCAAGAGACCACAAACCCATGAGCAGTAAGGAAAGGAGACGCATTGAAGCATCGGGTGGACATGTATTCGATGGCTATCTAAATGGACAACTTAATGTGGCTCGAGCGCTAGGTGACTTTCATATGGAAGGCATGAAGAAGAAGAAAGATGGTTCTGATTGTGGACCTCTAATTGCAGAGCCTGAGCTCATGACAACAAAACTAACAGAAGAGGATGAGTTCCTTATAATTGGGTGTGACGGGGTTTGGGATGTGTTCATGAGCCAGAATGCTGTAGATTTTGCCAGAAGGAGACTACAGGAGCACAATGACCCGGTCATGTGTAGTAAGGAGCTGGTTGAGGAAGCTTTGAAGAGGAAGAGTGCTGATAATGTGACGGCAGTGGTTGTGTGTCTTCAGCCACAGCCACCACCGAACTTGGTAGCGCCGAGGTTGAGAGTTCACAGAAGCTTCTCGGCGGAGGGTTTAAAAGATTTACAGAGCTACTTGGATGGCTTGGGAAACTAA

Protein Analysis

392

Amino Acids

42.67

Weight (kDa)

5.39

Isoelectric Point (pI)

61.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 97 - 349 7.6e-70 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 899
AasI GACNNNNNNGTC 1 cut(s) 196
AccB1I GGYRCC 1 cut(s) 142
AccB7I CCANNNNNTGG 1 cut(s) 35
AccII CGCG 1 cut(s) 265
AciI CCGC 6 cut(s) 54, 62, 93, 96, 420, 1130
AclWI GGATC 1 cut(s) 20
AcsI RAATTY 2 cut(s) 18, 358
AcuI CTGAAG 3 cut(s) 486, 549, 1052
AcyI GRCGYC 1 cut(s) 143
AfaI GTAC 1 cut(s) 452
AfeI AGCGCT 1 cut(s) 780
AfiI CCNNNNNNNGG 3 cut(s) 35, 111, 387
AflIII ACRYGT 2 cut(s) 263, 733
AgsI TTSAA 5 cut(s) 461, 515, 668, 719, 1024
AjnI CCWGG 1 cut(s) 293
AluBI AGCT 8 cut(s) 275, 345, 587, 859, 1006, 1019, 1122, 1155
AluI AGCT 8 cut(s) 275, 345, 587, 859, 1006, 1019, 1122, 1155
Alw21I GWGCWC 2 cut(s) 861, 978
Alw26I GTCTC 3 cut(s) 672, 703, 958
AlwI GGATC 1 cut(s) 20
AlwNI CAGNNNCTG 2 cut(s) 524, 854
Ama87I CYCGRG 1 cut(s) 774
Aor51HI AGCGCT 1 cut(s) 780
AoxI GGCC 1 cut(s) 349
ApeKI GCWGC 3 cut(s) 98, 101, 584
ApoI RAATTY 2 cut(s) 18, 358
AspLEI GCGC 3 cut(s) 145, 781, 1099
AspS9I GGNCC 2 cut(s) 371, 836
AsuC2I CCSGG 1 cut(s) 986
AsuHPI GGTGA 2 cut(s) 428, 797
AvaI CYCGRG 1 cut(s) 774
AvaII GGWCC 2 cut(s) 371, 836
BanI GGYRCC 1 cut(s) 142
BanII GRGCYC 1 cut(s) 861
BauI CACGAG 1 cut(s) 182
BbsI GAAGAC 2 cut(s) 194, 1058
Bbv12I GWGCWC 2 cut(s) 861, 978
BbvI GCAGC 3 cut(s) 85, 88, 596
BccI CCATC 5 cut(s) 392, 545, 737, 815, 1157
BceAI ACGGC 5 cut(s) 67, 89, 130, 142, 1065
BciT130I CCWGG 1 cut(s) 295
BcnI CCSGG 1 cut(s) 986
BcoDI GTCTC 3 cut(s) 672, 703, 958
BfaI CTAG 3 cut(s) 12, 477, 782
BfmI CTRYAG 3 cut(s) 228, 945, 968
BfoI RGCGCY 3 cut(s) 146, 782, 1100
BisI GCNGC 5 cut(s) 96, 99, 102, 420, 585
BlsI GCNGC 5 cut(s) 97, 100, 103, 421, 586
Bme1390I CCNGG 2 cut(s) 295, 986
Bme18I GGWCC 2 cut(s) 371, 836
BmeT110I CYCGRG 1 cut(s) 774
BmgT120I GGNCC 2 cut(s) 371, 836
BmiI GGNNCC 3 cut(s) 144, 292, 557
BmrFI CCNGG 2 cut(s) 295, 986
BmsI GCATC 2 cut(s) 301, 731
BpiI GAAGAC 2 cut(s) 194, 1058
BpmI CTGGAG 3 cut(s) 277, 291, 645
Bpu10I CCTNAGC 1 cut(s) 855
BpuMI CCSGG 1 cut(s) 986
BsaBI GATNNNNATC 2 cut(s) 315, 747
BsaHI GRCGYC 1 cut(s) 143
BsaI GGTCTC 1 cut(s) 672
BsaJI CCNNGG 2 cut(s) 446, 1100
BsaXI ACNNNNNCTCC 2 cut(s) 31, 61
Bsc4I CCNNNNNNNGG 3 cut(s) 35, 111, 387
Bse1I ACTGG 1 cut(s) 168
Bse8I GATNNNNATC 2 cut(s) 315, 747
BseBI CCWGG 1 cut(s) 295
BseDI CCNNGG 2 cut(s) 446, 1100
BseGI GGATG 4 cut(s) 556, 892, 928, 1168
BseJI GATNNNNATC 2 cut(s) 315, 747
BseLI CCNNNNNNNGG 3 cut(s) 35, 111, 387
BseMII CTCAG 4 cut(s) 237, 354, 414, 846
BseNI ACTGG 1 cut(s) 168
BseXI GCAGC 3 cut(s) 85, 88, 596
Bsh1236I CGCG 1 cut(s) 265
Bsh1285I CGRYCG 1 cut(s) 137
BshFI GGCC 1 cut(s) 351
BshNI GGYRCC 1 cut(s) 142
BsiEI CGRYCG 1 cut(s) 137
BsiHKAI GWGCWC 2 cut(s) 861, 978
BsiHKCI CYCGRG 1 cut(s) 774
BsiSI CCGG 1 cut(s) 986
BslI CCNNNNNNNGG 3 cut(s) 35, 111, 387
BsmAI GTCTC 3 cut(s) 672, 703, 958
BsmBI CGTCTC 1 cut(s) 703
BsmI GAATGC 1 cut(s) 946
BsnI GGCC 1 cut(s) 351
Bso31I GGTCTC 1 cut(s) 672
BsoBI CYCGRG 1 cut(s) 774
Bsp1286I GDGCHC 2 cut(s) 861, 978
Bsp143I GATC 3 cut(s) 25, 134, 463
BspACI CCGC 6 cut(s) 54, 62, 93, 96, 420, 1130
BspANI GGCC 1 cut(s) 351
BspCNI CTCAG 4 cut(s) 238, 355, 415, 847
BspFNI CGCG 1 cut(s) 265
BspHI TCATGA 2 cut(s) 861, 930
BspLI GGNNCC 3 cut(s) 144, 292, 557
BspPI GGATC 1 cut(s) 20
BspT107I GGYRCC 1 cut(s) 142
BspTNI GGTCTC 1 cut(s) 672
BsrI ACTGG 1 cut(s) 168
BssECI CCNNGG 2 cut(s) 446, 1100
BssMI GATC 3 cut(s) 25, 134, 463
BssNI GRCGYC 1 cut(s) 143
BssSI CACGAG 1 cut(s) 182
Bst2BI CACGAG 1 cut(s) 182
Bst2UI CCWGG 1 cut(s) 295
Bst6I CTCTTC 4 cut(s) 88, 876, 1019, 1025
BstACI GRCGYC 1 cut(s) 143
BstC8I GCNNGC 2 cut(s) 131, 417
BstDEI CTNAG 5 cut(s) 246, 363, 423, 532, 855
BstF5I GGATG 4 cut(s) 556, 892, 928, 1168
BstFNI CGCG 1 cut(s) 265
BstH2I RGCGCY 3 cut(s) 146, 782, 1100
BstHHI GCGC 3 cut(s) 145, 781, 1099
BstKTI GATC 3 cut(s) 28, 137, 466
BstMAI GTCTC 3 cut(s) 672, 703, 958
BstMBI GATC 3 cut(s) 25, 134, 463
BstMCI CGRYCG 1 cut(s) 137
BstMWI GCNNNNNNNGC 3 cut(s) 101, 428, 584
BstNI CCWGG 1 cut(s) 295
BstNSI RCATGY 3 cut(s) 419, 435, 737
BstSCI CCNGG 2 cut(s) 293, 984
BstSFI CTRYAG 3 cut(s) 228, 945, 968
BstUI CGCG 1 cut(s) 265
BstV1I GCAGC 3 cut(s) 85, 88, 596
BstV2I GAAGAC 2 cut(s) 194, 1058
BsuRI GGCC 1 cut(s) 351
BtsCI GGATG 4 cut(s) 556, 892, 928, 1168
BtsI GCAGTG 2 cut(s) 522, 1059
BtsIMutI CAGTG 3 cut(s) 161, 522, 1059
Cac8I GCNNGC 2 cut(s) 131, 417
CaiI CAGNNNCTG 2 cut(s) 524, 854
CciI TCATGA 2 cut(s) 861, 930
CfoI GCGC 3 cut(s) 145, 781, 1099
Cfr13I GGNCC 2 cut(s) 371, 836
CseI GACGC 1 cut(s) 720
Csp6I GTAC 1 cut(s) 451
CspCI CAANNNNNGTGG 2 cut(s) 1071, 1106
CviQI GTAC 1 cut(s) 451
DdeI CTNAG 5 cut(s) 246, 363, 423, 532, 855
DinI GGCGCC 1 cut(s) 144
DpnI GATC 3 cut(s) 27, 136, 465
DpnII GATC 3 cut(s) 25, 134, 463
DraI TTTAAA 1 cut(s) 1140
DrdI GACNNNNNNGTC 1 cut(s) 196
DseDI GACNNNNNNGTC 1 cut(s) 196
Eam1104I CTCTTC 4 cut(s) 88, 876, 1019, 1025
EarI CTCTTC 4 cut(s) 88, 876, 1019, 1025
EciI GGCGGA 2 cut(s) 82, 1145
Ecl136II GAGCTC 1 cut(s) 859
Eco24I GRGCYC 1 cut(s) 861
Eco31I GGTCTC 1 cut(s) 672
Eco47I GGWCC 2 cut(s) 371, 836
Eco47III AGCGCT 1 cut(s) 780
Eco53kI GAGCTC 1 cut(s) 859
Eco57I CTGAAG 3 cut(s) 486, 549, 1052
Eco88I CYCGRG 1 cut(s) 774
EcoICRI GAGCTC 1 cut(s) 859
EcoRI GAATTC 1 cut(s) 358
EcoRII CCWGG 1 cut(s) 293
EcoT38I GRGCYC 1 cut(s) 861
EgeI GGCGCC 1 cut(s) 144
EheI GGCGCC 1 cut(s) 144
Esp3I CGTCTC 1 cut(s) 703
FauNDI CATATG 1 cut(s) 795
Fnu4HI GCNGC 5 cut(s) 96, 99, 102, 420, 585
FokI GGATG 4 cut(s) 563, 899, 935, 1175
FriOI GRGCYC 1 cut(s) 861
Fsp4HI GCNGC 5 cut(s) 96, 99, 102, 420, 585
FspBI CTAG 3 cut(s) 12, 477, 782
GlaI GCGC 3 cut(s) 144, 780, 1098
GluI GCNGC 5 cut(s) 96, 99, 102, 420, 585
GsuI CTGGAG 3 cut(s) 277, 291, 645
HaeII RGCGCY 3 cut(s) 146, 782, 1100
HaeIII GGCC 1 cut(s) 351
HapII CCGG 1 cut(s) 986
HgaI GACGC 1 cut(s) 720
HhaI GCGC 3 cut(s) 145, 781, 1099
Hin1I GRCGYC 1 cut(s) 143
Hin6I GCGC 3 cut(s) 143, 779, 1097
HinP1I GCGC 3 cut(s) 143, 779, 1097
HindIII AAGCTT 2 cut(s) 1017, 1120
HinfI GANTC 1 cut(s) 67
HpaII CCGG 1 cut(s) 986
HphI GGTGA 2 cut(s) 428, 797
Hpy166II GTNNAC 6 cut(s) 59, 109, 225, 731, 836, 1114
Hpy188I TCNGA 7 cut(s) 25, 202, 247, 283, 357, 364, 829
Hpy188III TCNNGA 8 cut(s) 29, 184, 270, 467, 567, 675, 862, 931
Hpy8I GTNNAC 6 cut(s) 59, 109, 225, 731, 836, 1114
Hpy99I CGWCG 1 cut(s) 128
HpyAV CCTTC 8 cut(s) 88, 130, 362, 391, 538, 594, 794, 954
HpyCH4V TGCA 3 cut(s) 431, 633, 848
HpyF10VI GCNNNNNNNGC 3 cut(s) 101, 428, 584
HpyF3I CTNAG 5 cut(s) 246, 363, 423, 532, 855
Hsp92I GRCGYC 1 cut(s) 143
HspAI GCGC 3 cut(s) 143, 779, 1097
KasI GGCGCC 1 cut(s) 142
Kzo9I GATC 3 cut(s) 25, 134, 463
LmnI GCTCC 5 cut(s) 272, 296, 555, 973, 1003
Lsp1109I GCAGC 3 cut(s) 85, 88, 596
LweI GCATC 2 cut(s) 301, 731
MaeI CTAG 3 cut(s) 12, 477, 782
MaeIII GTNAC 6 cut(s) 47, 159, 434, 785, 908, 1045
MalI GATC 3 cut(s) 27, 136, 465
MboI GATC 3 cut(s) 25, 134, 463
MhlI GDGCHC 2 cut(s) 861, 978
MluCI AATT 5 cut(s) 18, 331, 358, 843, 900
MluI ACGCGT 1 cut(s) 263
Mly113I GGCGCC 1 cut(s) 143
MseI TTAA 2 cut(s) 765, 1139
MslI CAYNNNNRTG 1 cut(s) 668
MspA1I CMGCKG 2 cut(s) 98, 422
MspI CCGG 1 cut(s) 986
MspR9I CCNGG 2 cut(s) 295, 986
Mva1269I GAATGC 1 cut(s) 946
MvaI CCWGG 1 cut(s) 295
MvnI CGCG 1 cut(s) 265
MwoI GCNNNNNNNGC 3 cut(s) 101, 428, 584
NarI GGCGCC 1 cut(s) 143
NciI CCSGG 1 cut(s) 986
NdeI CATATG 1 cut(s) 795
NdeII GATC 3 cut(s) 25, 134, 463
NlaIV GGNNCC 3 cut(s) 144, 292, 557
NmeAIII GCCGAG 2 cut(s) 1106, 1125
NmuCI GTSAC 5 cut(s) 159, 434, 785, 908, 1045
NspI RCATGY 3 cut(s) 419, 435, 737
PaeI GCATGC 1 cut(s) 419
PaeR7I CTCGAG 1 cut(s) 774
PagI TCATGA 2 cut(s) 861, 930
PciI ACATGT 1 cut(s) 733
PcsI WCGNNNNNNNCGW 1 cut(s) 143
PctI GAATGC 1 cut(s) 946
PfeI GAWTC 1 cut(s) 67
PflFI GACNNNGTC 1 cut(s) 986
PflMI CCANNNNNTGG 1 cut(s) 35
PkrI GCNGC 5 cut(s) 97, 100, 103, 421, 586
Ple19I CGATCG 1 cut(s) 137
PluTI GGCGCC 1 cut(s) 146
PscI ACATGT 1 cut(s) 733
PsiI TTATAA 1 cut(s) 899
Psp124BI GAGCTC 1 cut(s) 861
Psp6I CCWGG 1 cut(s) 293
PspGI CCWGG 1 cut(s) 293
PspN4I GGNNCC 3 cut(s) 144, 292, 557
PspPI GGNCC 2 cut(s) 371, 836
PspXI VCTCGAGB 1 cut(s) 774
PstNI CAGNNNCTG 2 cut(s) 524, 854
PsyI GACNNNGTC 1 cut(s) 986
PvuI CGATCG 1 cut(s) 137
RsaI GTAC 1 cut(s) 452
RsaNI GTAC 1 cut(s) 451
RseI CAYNNNNRTG 1 cut(s) 668
SacI GAGCTC 1 cut(s) 861
SaqAI TTAA 2 cut(s) 765, 1139
SatI GCNGC 5 cut(s) 96, 99, 102, 420, 585
Sau3AI GATC 3 cut(s) 25, 134, 463
Sau96I GGNCC 2 cut(s) 371, 836
ScrFI CCNGG 2 cut(s) 295, 986
SduI GDGCHC 2 cut(s) 861, 978
SfaNI GCATC 2 cut(s) 301, 731
SfcI CTRYAG 3 cut(s) 228, 945, 968
SfoI GGCGCC 1 cut(s) 144
Sfr274I CTCGAG 1 cut(s) 774
SinI GGWCC 2 cut(s) 371, 836
SlaI CTCGAG 1 cut(s) 774
SmiMI CAYNNNNRTG 1 cut(s) 668
SmlI CTYRAG 1 cut(s) 774
SmoI CTYRAG 1 cut(s) 774
SphI GCATGC 1 cut(s) 419
Sse9I AATT 5 cut(s) 18, 331, 358, 843, 900
SsiI CCGC 6 cut(s) 54, 62, 93, 96, 420, 1130
SspDI GGCGCC 1 cut(s) 142
SspMI CTAG 3 cut(s) 12, 477, 782
SstI GAGCTC 1 cut(s) 861
StyD4I CCNGG 2 cut(s) 293, 984
TaqI TCGA 5 cut(s) 44, 123, 169, 741, 775
TasI AATT 5 cut(s) 18, 331, 358, 843, 900
TauI GCSGC 2 cut(s) 98, 422
TfiI GAWTC 1 cut(s) 67
Tru1I TTAA 2 cut(s) 765, 1139
Tru9I TTAA 2 cut(s) 765, 1139
TscAI CASTG 3 cut(s) 168, 529, 1059
TseFI GTSAC 5 cut(s) 159, 434, 785, 908, 1045
TseI GCWGC 3 cut(s) 98, 101, 584
Tsp45I GTSAC 5 cut(s) 159, 434, 785, 908, 1045
TspDTI ATGAA 5 cut(s) 325, 534, 782, 821, 919
TspRI CASTG 3 cut(s) 168, 529, 1059
Tth111I GACNNNGTC 1 cut(s) 986
Van91I CCANNNNNTGG 1 cut(s) 35
VpaK11BI GGWCC 2 cut(s) 371, 836
XapI RAATTY 2 cut(s) 18, 358
XceI RCATGY 3 cut(s) 419, 435, 737
XcmI CCANNNNNNNNNTGG 1 cut(s) 301
XhoI CTCGAG 1 cut(s) 774
XspI CTAG 3 cut(s) 12, 477, 782
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.